Full text data of ANXA4
ANXA4
(ANX4)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Annexin A4 (35-beta calcimedin; Annexin IV; Annexin-4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; P32.5; PP4-X; Placental anticoagulant protein II; PAP-II; Protein II)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Annexin A4 (35-beta calcimedin; Annexin IV; Annexin-4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; Lipocortin IV; P32.5; PP4-X; Placental anticoagulant protein II; PAP-II; Protein II)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
hRBCD
IPI00221225
IPI00221225 ANXA4 protein ANXA4 protein membrane n/a n/a n/a n/a n/a n/a n/a n/a 5 n/a n/a n/a n/a n/a n/a n/a n/a 2 n/a n/a likely membrane bound and cytoskeleton n/a found at its expected molecular weight found at molecular weight
IPI00221225 ANXA4 protein ANXA4 protein membrane n/a n/a n/a n/a n/a n/a n/a n/a 5 n/a n/a n/a n/a n/a n/a n/a n/a 2 n/a n/a likely membrane bound and cytoskeleton n/a found at its expected molecular weight found at molecular weight
UniProt
P09525
ID ANXA4_HUMAN Reviewed; 319 AA.
AC P09525; Q96F33; Q9BWK1;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 4.
DT 22-JAN-2014, entry version 151.
DE RecName: Full=Annexin A4;
DE AltName: Full=35-beta calcimedin;
DE AltName: Full=Annexin IV;
DE AltName: Full=Annexin-4;
DE AltName: Full=Carbohydrate-binding protein p33/p41;
DE AltName: Full=Chromobindin-4;
DE AltName: Full=Endonexin I;
DE AltName: Full=Lipocortin IV;
DE AltName: Full=P32.5;
DE AltName: Full=PP4-X;
DE AltName: Full=Placental anticoagulant protein II;
DE Short=PAP-II;
DE AltName: Full=Protein II;
GN Name=ANXA4; Synonyms=ANX4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2970257;
RA Grundmann U., Amann E., Abel K.-J., Kuepper H.A.;
RT "Isolation and expression of cDNA coding for a new member of the
RT phospholipase A2 inhibitor family.";
RL Behring Inst. Mitt. 82:59-67(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1346776; DOI=10.1016/0888-7543(92)90379-7;
RA Tait J.F., Smith C., Frankenberry D.A., Miao C.H., Adler D.A.,
RA Disteche C.M.;
RT "Chromosomal mapping of the human annexin IV (ANX4) gene.";
RL Genomics 12:313-318(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9084877;
RA Satoh A., Takayama E., Kojima K., Ogawa H., Katsura Y., Kina T.,
RA Matsumoto I.;
RT "Characterization of human p33/41 (annexin IV), a Ca2+ dependent
RT carbohydrate-binding protein with monoclonal anti-annexin IV
RT antibodies, AS11 and AS17.";
RL Biol. Pharm. Bull. 20:224-229(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PROTEIN SEQUENCE OF 2-15; 28-72; 100-144 AND 281-319.
RX PubMed=2975506; DOI=10.1021/bi00417a011;
RA Tait J.F., Sakata M., McMullen B.A., Miao C.H., Funakoshi T.,
RA Hendrickson L.E., Fujikawa K.;
RT "Placental anticoagulant proteins: isolation and comparative
RT characterization four members of the lipocortin family.";
RL Biochemistry 27:6268-6276(1988).
RN [7]
RP PROTEIN SEQUENCE OF 27-56; 99-124 AND 280-308.
RC TISSUE=Placenta;
RX PubMed=2974032;
RA Ahn N.G., Teller D.C., Bienkowski M.J., McMullen B.A., Lipkin E.W.,
RA de Haen C.;
RT "Sedimentation equilibrium analysis of five lipocortin-related
RT phospholipase A2 inhibitors from human placenta. Evidence against a
RT mechanistically relevant association between enzyme and inhibitor.";
RL J. Biol. Chem. 263:18657-18663(1988).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-213; LYS-293 AND LYS-300,
RP AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX PubMed=2254922; DOI=10.1016/S0022-2836(05)80310-9;
RA Freemont P.S., Driessen H.P.C., Verbi W., Crumpton M.J.;
RT "Crystallization and preliminary X-ray crystallographic studies of
RT human placental annexin IV.";
RL J. Mol. Biol. 216:219-221(1990).
CC -!- FUNCTION: Calcium/phospholipid-binding protein which promotes
CC membrane fusion and is involved in exocytosis (By similarity).
CC -!- DOMAIN: A pair of annexin repeats may form one binding site for
CC calcium and phospholipid.
CC -!- MISCELLANEOUS: Seems to bind one calcium ion with high affinity.
CC -!- SIMILARITY: Belongs to the annexin family.
CC -!- SIMILARITY: Contains 4 annexin repeats.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC41689.1; Type=Erroneous initiation;
CC Sequence=AAH00182.1; Type=Erroneous initiation;
CC Sequence=AAH11659.1; Type=Erroneous initiation;
CC Sequence=BAA11227.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M19383; AAC41689.1; ALT_INIT; mRNA.
DR EMBL; M82809; AAA51740.1; -; mRNA.
DR EMBL; D78152; BAA11227.1; ALT_INIT; mRNA.
DR EMBL; AC019206; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC092431; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC112787; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC000182; AAH00182.1; ALT_INIT; mRNA.
DR EMBL; BC011659; AAH11659.1; ALT_INIT; mRNA.
DR PIR; A42077; A42077.
DR RefSeq; NP_001144.1; NM_001153.3.
DR RefSeq; XP_005264334.1; XM_005264277.1.
DR RefSeq; XP_005264335.1; XM_005264278.1.
DR UniGene; Hs.422986; -.
DR PDB; 2ZOC; X-ray; 2.00 A; A/B=1-319.
DR PDBsum; 2ZOC; -.
DR ProteinModelPortal; P09525; -.
DR SMR; P09525; 1-319.
DR IntAct; P09525; 4.
DR MINT; MINT-4528752; -.
DR STRING; 9606.ENSP00000377833; -.
DR PhosphoSite; P09525; -.
DR DMDM; 1703319; -.
DR DOSAC-COBS-2DPAGE; P09525; -.
DR OGP; P09525; -.
DR REPRODUCTION-2DPAGE; IPI00793199; -.
DR REPRODUCTION-2DPAGE; P09525; -.
DR SWISS-2DPAGE; P09525; -.
DR PaxDb; P09525; -.
DR PRIDE; P09525; -.
DR DNASU; 307; -.
DR Ensembl; ENST00000394295; ENSP00000377833; ENSG00000196975.
DR GeneID; 307; -.
DR KEGG; hsa:307; -.
DR UCSC; uc010yqo.2; human.
DR CTD; 307; -.
DR GeneCards; GC02P069872; -.
DR HGNC; HGNC:542; ANXA4.
DR HPA; CAB005076; -.
DR HPA; CAB017560; -.
DR HPA; HPA007393; -.
DR MIM; 106491; gene.
DR neXtProt; NX_P09525; -.
DR PharmGKB; PA24832; -.
DR eggNOG; NOG267770; -.
DR HOVERGEN; HBG061815; -.
DR InParanoid; P09525; -.
DR KO; K17093; -.
DR EvolutionaryTrace; P09525; -.
DR GeneWiki; ANXA4; -.
DR GenomeRNAi; 307; -.
DR NextBio; 1239; -.
DR PRO; PR:P09525; -.
DR ArrayExpress; P09525; -.
DR Bgee; P09525; -.
DR CleanEx; HS_ANXA4; -.
DR Genevestigator; P09525; -.
DR GO; GO:0005737; C:cytoplasm; TAS:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; NAS:UniProtKB.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IDA:UniProtKB.
DR GO; GO:0004859; F:phospholipase inhibitor activity; NAS:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; TAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; TAS:UniProtKB.
DR Gene3D; 1.10.220.10; -; 4.
DR InterPro; IPR001464; Annexin.
DR InterPro; IPR018502; Annexin_repeat.
DR InterPro; IPR018252; Annexin_repeat_CS.
DR InterPro; IPR002391; AnnexinIV.
DR PANTHER; PTHR10502:SF28; PTHR10502:SF28; 1.
DR Pfam; PF00191; Annexin; 4.
DR PRINTS; PR00196; ANNEXIN.
DR SMART; SM00335; ANX; 4.
DR PROSITE; PS00223; ANNEXIN; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Annexin; Calcium;
KW Calcium/phospholipid-binding; Complete proteome;
KW Direct protein sequencing; Phosphoprotein; Polymorphism;
KW Reference proteome; Repeat.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 319 Annexin A4.
FT /FTId=PRO_0000067482.
FT REPEAT 23 83 Annexin 1.
FT REPEAT 95 155 Annexin 2.
FT REPEAT 179 239 Annexin 3.
FT REPEAT 254 314 Annexin 4.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 7 7 Phosphothreonine (By similarity).
FT MOD_RES 213 213 N6-acetyllysine.
FT MOD_RES 293 293 N6-acetyllysine.
FT MOD_RES 300 300 N6-acetyllysine.
FT VARIANT 85 85 T -> M (in dbSNP:rs2228203).
FT /FTId=VAR_055500.
FT CONFLICT 96 96 R -> Q (in Ref. 1; AAC41689).
FT HELIX 16 26
FT STRAND 28 31
FT HELIX 34 41
FT HELIX 46 59
FT HELIX 64 71
FT HELIX 74 84
FT HELIX 87 99
FT STRAND 100 103
FT HELIX 106 115
FT HELIX 118 132
FT HELIX 136 143
FT HELIX 146 155
FT TURN 156 158
FT HELIX 168 181
FT TURN 182 184
FT STRAND 185 187
FT HELIX 190 199
FT HELIX 202 216
FT HELIX 220 227
FT HELIX 230 244
FT HELIX 246 258
FT STRAND 259 262
FT HELIX 265 275
FT TURN 276 279
FT HELIX 280 291
FT HELIX 295 302
FT HELIX 305 315
SQ SEQUENCE 319 AA; 35883 MW; 3799FE80A93AB215 CRC64;
MATKGGTVKA ASGFNAMEDA QTLRKAMKGL GTDEDAIISV LAYRNTAQRQ EIRTAYKSTI
GRDLIDDLKS ELSGNFEQVI VGMMTPTVLY DVQELRRAMK GAGTDEGCLI EILASRTPEE
IRRISQTYQQ QYGRSLEDDI RSDTSFMFQR VLVSLSAGGR DEGNYLDDAL VRQDAQDLYE
AGEKKWGTDE VKFLTVLCSR NRNHLLHVFD EYKRISQKDI EQSIKSETSG SFEDALLAIV
KCMRNKSAYF AEKLYKSMKG LGTDDNTLIR VMVSRAEIDM LDIRAHFKRL YGKSLYSFIK
GDTSGDYRKV LLVLCGGDD
//
ID ANXA4_HUMAN Reviewed; 319 AA.
AC P09525; Q96F33; Q9BWK1;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 4.
DT 22-JAN-2014, entry version 151.
DE RecName: Full=Annexin A4;
DE AltName: Full=35-beta calcimedin;
DE AltName: Full=Annexin IV;
DE AltName: Full=Annexin-4;
DE AltName: Full=Carbohydrate-binding protein p33/p41;
DE AltName: Full=Chromobindin-4;
DE AltName: Full=Endonexin I;
DE AltName: Full=Lipocortin IV;
DE AltName: Full=P32.5;
DE AltName: Full=PP4-X;
DE AltName: Full=Placental anticoagulant protein II;
DE Short=PAP-II;
DE AltName: Full=Protein II;
GN Name=ANXA4; Synonyms=ANX4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2970257;
RA Grundmann U., Amann E., Abel K.-J., Kuepper H.A.;
RT "Isolation and expression of cDNA coding for a new member of the
RT phospholipase A2 inhibitor family.";
RL Behring Inst. Mitt. 82:59-67(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1346776; DOI=10.1016/0888-7543(92)90379-7;
RA Tait J.F., Smith C., Frankenberry D.A., Miao C.H., Adler D.A.,
RA Disteche C.M.;
RT "Chromosomal mapping of the human annexin IV (ANX4) gene.";
RL Genomics 12:313-318(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9084877;
RA Satoh A., Takayama E., Kojima K., Ogawa H., Katsura Y., Kina T.,
RA Matsumoto I.;
RT "Characterization of human p33/41 (annexin IV), a Ca2+ dependent
RT carbohydrate-binding protein with monoclonal anti-annexin IV
RT antibodies, AS11 and AS17.";
RL Biol. Pharm. Bull. 20:224-229(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PROTEIN SEQUENCE OF 2-15; 28-72; 100-144 AND 281-319.
RX PubMed=2975506; DOI=10.1021/bi00417a011;
RA Tait J.F., Sakata M., McMullen B.A., Miao C.H., Funakoshi T.,
RA Hendrickson L.E., Fujikawa K.;
RT "Placental anticoagulant proteins: isolation and comparative
RT characterization four members of the lipocortin family.";
RL Biochemistry 27:6268-6276(1988).
RN [7]
RP PROTEIN SEQUENCE OF 27-56; 99-124 AND 280-308.
RC TISSUE=Placenta;
RX PubMed=2974032;
RA Ahn N.G., Teller D.C., Bienkowski M.J., McMullen B.A., Lipkin E.W.,
RA de Haen C.;
RT "Sedimentation equilibrium analysis of five lipocortin-related
RT phospholipase A2 inhibitors from human placenta. Evidence against a
RT mechanistically relevant association between enzyme and inhibitor.";
RL J. Biol. Chem. 263:18657-18663(1988).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-213; LYS-293 AND LYS-300,
RP AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX PubMed=2254922; DOI=10.1016/S0022-2836(05)80310-9;
RA Freemont P.S., Driessen H.P.C., Verbi W., Crumpton M.J.;
RT "Crystallization and preliminary X-ray crystallographic studies of
RT human placental annexin IV.";
RL J. Mol. Biol. 216:219-221(1990).
CC -!- FUNCTION: Calcium/phospholipid-binding protein which promotes
CC membrane fusion and is involved in exocytosis (By similarity).
CC -!- DOMAIN: A pair of annexin repeats may form one binding site for
CC calcium and phospholipid.
CC -!- MISCELLANEOUS: Seems to bind one calcium ion with high affinity.
CC -!- SIMILARITY: Belongs to the annexin family.
CC -!- SIMILARITY: Contains 4 annexin repeats.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC41689.1; Type=Erroneous initiation;
CC Sequence=AAH00182.1; Type=Erroneous initiation;
CC Sequence=AAH11659.1; Type=Erroneous initiation;
CC Sequence=BAA11227.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M19383; AAC41689.1; ALT_INIT; mRNA.
DR EMBL; M82809; AAA51740.1; -; mRNA.
DR EMBL; D78152; BAA11227.1; ALT_INIT; mRNA.
DR EMBL; AC019206; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC092431; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC112787; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC000182; AAH00182.1; ALT_INIT; mRNA.
DR EMBL; BC011659; AAH11659.1; ALT_INIT; mRNA.
DR PIR; A42077; A42077.
DR RefSeq; NP_001144.1; NM_001153.3.
DR RefSeq; XP_005264334.1; XM_005264277.1.
DR RefSeq; XP_005264335.1; XM_005264278.1.
DR UniGene; Hs.422986; -.
DR PDB; 2ZOC; X-ray; 2.00 A; A/B=1-319.
DR PDBsum; 2ZOC; -.
DR ProteinModelPortal; P09525; -.
DR SMR; P09525; 1-319.
DR IntAct; P09525; 4.
DR MINT; MINT-4528752; -.
DR STRING; 9606.ENSP00000377833; -.
DR PhosphoSite; P09525; -.
DR DMDM; 1703319; -.
DR DOSAC-COBS-2DPAGE; P09525; -.
DR OGP; P09525; -.
DR REPRODUCTION-2DPAGE; IPI00793199; -.
DR REPRODUCTION-2DPAGE; P09525; -.
DR SWISS-2DPAGE; P09525; -.
DR PaxDb; P09525; -.
DR PRIDE; P09525; -.
DR DNASU; 307; -.
DR Ensembl; ENST00000394295; ENSP00000377833; ENSG00000196975.
DR GeneID; 307; -.
DR KEGG; hsa:307; -.
DR UCSC; uc010yqo.2; human.
DR CTD; 307; -.
DR GeneCards; GC02P069872; -.
DR HGNC; HGNC:542; ANXA4.
DR HPA; CAB005076; -.
DR HPA; CAB017560; -.
DR HPA; HPA007393; -.
DR MIM; 106491; gene.
DR neXtProt; NX_P09525; -.
DR PharmGKB; PA24832; -.
DR eggNOG; NOG267770; -.
DR HOVERGEN; HBG061815; -.
DR InParanoid; P09525; -.
DR KO; K17093; -.
DR EvolutionaryTrace; P09525; -.
DR GeneWiki; ANXA4; -.
DR GenomeRNAi; 307; -.
DR NextBio; 1239; -.
DR PRO; PR:P09525; -.
DR ArrayExpress; P09525; -.
DR Bgee; P09525; -.
DR CleanEx; HS_ANXA4; -.
DR Genevestigator; P09525; -.
DR GO; GO:0005737; C:cytoplasm; TAS:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; NAS:UniProtKB.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IDA:UniProtKB.
DR GO; GO:0004859; F:phospholipase inhibitor activity; NAS:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; TAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; TAS:UniProtKB.
DR Gene3D; 1.10.220.10; -; 4.
DR InterPro; IPR001464; Annexin.
DR InterPro; IPR018502; Annexin_repeat.
DR InterPro; IPR018252; Annexin_repeat_CS.
DR InterPro; IPR002391; AnnexinIV.
DR PANTHER; PTHR10502:SF28; PTHR10502:SF28; 1.
DR Pfam; PF00191; Annexin; 4.
DR PRINTS; PR00196; ANNEXIN.
DR SMART; SM00335; ANX; 4.
DR PROSITE; PS00223; ANNEXIN; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Annexin; Calcium;
KW Calcium/phospholipid-binding; Complete proteome;
KW Direct protein sequencing; Phosphoprotein; Polymorphism;
KW Reference proteome; Repeat.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 319 Annexin A4.
FT /FTId=PRO_0000067482.
FT REPEAT 23 83 Annexin 1.
FT REPEAT 95 155 Annexin 2.
FT REPEAT 179 239 Annexin 3.
FT REPEAT 254 314 Annexin 4.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 7 7 Phosphothreonine (By similarity).
FT MOD_RES 213 213 N6-acetyllysine.
FT MOD_RES 293 293 N6-acetyllysine.
FT MOD_RES 300 300 N6-acetyllysine.
FT VARIANT 85 85 T -> M (in dbSNP:rs2228203).
FT /FTId=VAR_055500.
FT CONFLICT 96 96 R -> Q (in Ref. 1; AAC41689).
FT HELIX 16 26
FT STRAND 28 31
FT HELIX 34 41
FT HELIX 46 59
FT HELIX 64 71
FT HELIX 74 84
FT HELIX 87 99
FT STRAND 100 103
FT HELIX 106 115
FT HELIX 118 132
FT HELIX 136 143
FT HELIX 146 155
FT TURN 156 158
FT HELIX 168 181
FT TURN 182 184
FT STRAND 185 187
FT HELIX 190 199
FT HELIX 202 216
FT HELIX 220 227
FT HELIX 230 244
FT HELIX 246 258
FT STRAND 259 262
FT HELIX 265 275
FT TURN 276 279
FT HELIX 280 291
FT HELIX 295 302
FT HELIX 305 315
SQ SEQUENCE 319 AA; 35883 MW; 3799FE80A93AB215 CRC64;
MATKGGTVKA ASGFNAMEDA QTLRKAMKGL GTDEDAIISV LAYRNTAQRQ EIRTAYKSTI
GRDLIDDLKS ELSGNFEQVI VGMMTPTVLY DVQELRRAMK GAGTDEGCLI EILASRTPEE
IRRISQTYQQ QYGRSLEDDI RSDTSFMFQR VLVSLSAGGR DEGNYLDDAL VRQDAQDLYE
AGEKKWGTDE VKFLTVLCSR NRNHLLHVFD EYKRISQKDI EQSIKSETSG SFEDALLAIV
KCMRNKSAYF AEKLYKSMKG LGTDDNTLIR VMVSRAEIDM LDIRAHFKRL YGKSLYSFIK
GDTSGDYRKV LLVLCGGDD
//
MIM
106491
*RECORD*
*FIELD* NO
106491
*FIELD* TI
*106491 ANNEXIN A4; ANXA4
;;ANNEXIN IV; ANX4;;
PLACENTAL ANTICOAGULANT PROTEIN II;;
read moreZYMOGEN GRANULE MEMBRANE-ASSOCIATED PROTEIN, 36-KD; ZAP36
*FIELD* TX
CLONING
The annexins, or lipocortins, are a family of calcium-dependent
phospholipid-binding proteins whose normal function is uncertain.
Annexin IV, otherwise known as placental anticoagulant protein II, was 1
of the 4 annexins isolated from human placenta on the basis of their in
vitro anticoagulant activity. Grundmann et al. (1988) reported a cDNA
clone for annexin IV. Hauptmann et al. (1989) showed that annexin IV has
45 to 59% identity with other members of the annexin family.
Fukuoka et al. (2002) found that rat Anxa4, which they called Zap36, was
ubiquitously expressed in epithelial tissues. In dog and rat pancreas,
Zap36 associated with zymogen granule membranes.
GENE STRUCTURE
By genomic Southern blotting with a cDNA probe, Tait et al. (1992)
determined that the ANXA4 gene spans 18 to 56 kb. DNA sequence analysis
demonstrated a single intron with exon-intron boundaries in exactly the
same position as in the mouse annexin I and annexin II genes.
MAPPING
By PCR analysis of somatic cell hybrids and in situ hybridization with a
cDNA probe, Tait et al. (1992) mapped the human ANXA4 gene to chromosome
2p13. Barrow et al. (1994) demonstrated that the Anxa4 gene is located
on mouse chromosome 6 in a region of conserved synteny with human
chromosome 2.
*FIELD* RF
1. Barrow, L. L.; Simin, K.; Jones, J. M.; Lee, D. C.; Meisler, M.
H.: Conserved linkage of early growth response 4, annexin 4, and
transforming growth factor alpha on mouse chromosome 6. Genomics 19:
388-390, 1994.
2. Fukuoka, S.-I.; Kern, H.; Kazuki-Sugino, R.; Ikeda, Y.: Cloning
and characterization of ZAP36, an annexin-like, zymogen granule membrane
associated protein, in exocrine pancreas. Biochim. Biophys. Acta 1575:
148-152, 2002.
3. Grundmann, U.; Amann, E.; Abel, K.-J.; Kupper, H. A.: Isolation
and expression of cDNA coding for a new member of the phospholipase
A2 inhibitor family. Behring Inst. Mitt. 82: 59-67, 1988.
4. Hauptmann, R.; Maurer-Fogy, I.; Krystek, E.; Bodo, G.; Andree,
H.; Reutelingsperger, C. P. M.: Vascular anticoagulant beta, a novel
human Ca(2+)/phospholipid binding protein that inhibits coagulation
and phospholipase A-2 activity: its molecular cloning, expression
and comparison with VAC-alpha. Europ. J. Biochem. 185: 63-71, 1989.
5. Tait, J. F.; Smith, C.; Frankenberry, D. A.; Miao, C. H.; Adler,
D. A.; Disteche, C. M.: Chromosomal mapping of the human annexin
IV (ANX4) gene. Genomics 12: 313-318, 1992.
*FIELD* CN
Patricia A. Hartz - updated: 6/7/2005
*FIELD* CD
Victor A. McKusick: 2/1/1992
*FIELD* ED
wwang: 06/22/2005
mgross: 6/21/2005
terry: 6/7/2005
mgross: 9/17/1999
mark: 12/29/1996
joanna: 2/5/1996
mimadm: 3/11/1994
carol: 2/15/1994
supermim: 3/16/1992
carol: 2/26/1992
carol: 2/18/1992
carol: 2/1/1992
*RECORD*
*FIELD* NO
106491
*FIELD* TI
*106491 ANNEXIN A4; ANXA4
;;ANNEXIN IV; ANX4;;
PLACENTAL ANTICOAGULANT PROTEIN II;;
read moreZYMOGEN GRANULE MEMBRANE-ASSOCIATED PROTEIN, 36-KD; ZAP36
*FIELD* TX
CLONING
The annexins, or lipocortins, are a family of calcium-dependent
phospholipid-binding proteins whose normal function is uncertain.
Annexin IV, otherwise known as placental anticoagulant protein II, was 1
of the 4 annexins isolated from human placenta on the basis of their in
vitro anticoagulant activity. Grundmann et al. (1988) reported a cDNA
clone for annexin IV. Hauptmann et al. (1989) showed that annexin IV has
45 to 59% identity with other members of the annexin family.
Fukuoka et al. (2002) found that rat Anxa4, which they called Zap36, was
ubiquitously expressed in epithelial tissues. In dog and rat pancreas,
Zap36 associated with zymogen granule membranes.
GENE STRUCTURE
By genomic Southern blotting with a cDNA probe, Tait et al. (1992)
determined that the ANXA4 gene spans 18 to 56 kb. DNA sequence analysis
demonstrated a single intron with exon-intron boundaries in exactly the
same position as in the mouse annexin I and annexin II genes.
MAPPING
By PCR analysis of somatic cell hybrids and in situ hybridization with a
cDNA probe, Tait et al. (1992) mapped the human ANXA4 gene to chromosome
2p13. Barrow et al. (1994) demonstrated that the Anxa4 gene is located
on mouse chromosome 6 in a region of conserved synteny with human
chromosome 2.
*FIELD* RF
1. Barrow, L. L.; Simin, K.; Jones, J. M.; Lee, D. C.; Meisler, M.
H.: Conserved linkage of early growth response 4, annexin 4, and
transforming growth factor alpha on mouse chromosome 6. Genomics 19:
388-390, 1994.
2. Fukuoka, S.-I.; Kern, H.; Kazuki-Sugino, R.; Ikeda, Y.: Cloning
and characterization of ZAP36, an annexin-like, zymogen granule membrane
associated protein, in exocrine pancreas. Biochim. Biophys. Acta 1575:
148-152, 2002.
3. Grundmann, U.; Amann, E.; Abel, K.-J.; Kupper, H. A.: Isolation
and expression of cDNA coding for a new member of the phospholipase
A2 inhibitor family. Behring Inst. Mitt. 82: 59-67, 1988.
4. Hauptmann, R.; Maurer-Fogy, I.; Krystek, E.; Bodo, G.; Andree,
H.; Reutelingsperger, C. P. M.: Vascular anticoagulant beta, a novel
human Ca(2+)/phospholipid binding protein that inhibits coagulation
and phospholipase A-2 activity: its molecular cloning, expression
and comparison with VAC-alpha. Europ. J. Biochem. 185: 63-71, 1989.
5. Tait, J. F.; Smith, C.; Frankenberry, D. A.; Miao, C. H.; Adler,
D. A.; Disteche, C. M.: Chromosomal mapping of the human annexin
IV (ANX4) gene. Genomics 12: 313-318, 1992.
*FIELD* CN
Patricia A. Hartz - updated: 6/7/2005
*FIELD* CD
Victor A. McKusick: 2/1/1992
*FIELD* ED
wwang: 06/22/2005
mgross: 6/21/2005
terry: 6/7/2005
mgross: 9/17/1999
mark: 12/29/1996
joanna: 2/5/1996
mimadm: 3/11/1994
carol: 2/15/1994
supermim: 3/16/1992
carol: 2/26/1992
carol: 2/18/1992
carol: 2/1/1992