Full text data of ARF4
ARF4
(ARF2)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
ADP-ribosylation factor 4
Note: presumably soluble (membrane word is not in UniProt keywords or features)
ADP-ribosylation factor 4
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
P18085
ID ARF4_HUMAN Reviewed; 180 AA.
AC P18085; B2R7J7; P21371;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 3.
DT 22-JAN-2014, entry version 148.
DE RecName: Full=ADP-ribosylation factor 4;
GN Name=ARF4; Synonyms=ARF2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2107548; DOI=10.1073/pnas.87.6.2206;
RA Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J.,
RA Vaughan M.;
RT "Selective amplification of an mRNA and related pseudogene for a human
RT ADP-ribosylation factor, a guanine nucleotide-dependent protein
RT activator of cholera toxin.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1899243;
RA Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C.;
RT "Human ADP-ribosylation factors. A functionally conserved family of
RT GTP-binding proteins.";
RL J. Biol. Chem. 266:2606-2614(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10524252; DOI=10.1016/S0378-1119(99)00290-5;
RA Lebeda R.A., Haun R.S.;
RT "Cloning and characterization of the human ADP-ribosylation factor 4
RT gene.";
RL Gene 237:209-214(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction
RT sequenced by the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow, Placenta, Skin, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "Structure of human ADP-ribosylation factor 4.";
RL Submitted (APR-2005) to the PDB data bank.
CC -!- FUNCTION: GTP-binding protein that functions as an allosteric
CC activator of the cholera toxin catalytic subunit, an ADP-
CC ribosyltransferase. Involved in protein trafficking; may modulate
CC vesicle budding and uncoating within the Golgi apparatus.
CC -!- INTERACTION:
CC Q96PM5:RCHY1; NbExp=3; IntAct=EBI-1237085, EBI-947779;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC -!- CAUTION: Was originally thought to be ARF2.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M36341; AAA53081.1; -; mRNA.
DR EMBL; AF104238; AAD54674.1; -; Genomic_DNA.
DR EMBL; AF104233; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104234; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104235; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104236; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104237; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF493883; AAM12597.1; -; mRNA.
DR EMBL; AK313008; BAG35844.1; -; mRNA.
DR EMBL; BC003364; AAH03364.1; -; mRNA.
DR EMBL; BC008753; AAH08753.1; -; mRNA.
DR EMBL; BC016325; AAH16325.1; -; mRNA.
DR EMBL; BC022866; AAH22866.1; -; mRNA.
DR PIR; B38622; B38622.
DR RefSeq; NP_001651.1; NM_001660.3.
DR UniGene; Hs.652183; -.
DR PDB; 1Z6X; X-ray; 2.70 A; A/B=2-179.
DR PDBsum; 1Z6X; -.
DR ProteinModelPortal; P18085; -.
DR SMR; P18085; 4-178.
DR IntAct; P18085; 7.
DR MINT; MINT-2801449; -.
DR STRING; 9606.ENSP00000306010; -.
DR PhosphoSite; P18085; -.
DR DMDM; 114123; -.
DR PaxDb; P18085; -.
DR PRIDE; P18085; -.
DR DNASU; 378; -.
DR Ensembl; ENST00000303436; ENSP00000306010; ENSG00000168374.
DR GeneID; 378; -.
DR KEGG; hsa:378; -.
DR UCSC; uc003dix.4; human.
DR CTD; 378; -.
DR GeneCards; GC03M057532; -.
DR HGNC; HGNC:655; ARF4.
DR MIM; 601177; gene.
DR neXtProt; NX_P18085; -.
DR PharmGKB; PA24937; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000163691; -.
DR HOVERGEN; HBG002073; -.
DR InParanoid; P18085; -.
DR KO; K07939; -.
DR OMA; GADELQK; -.
DR OrthoDB; EOG77WWDV; -.
DR PhylomeDB; P18085; -.
DR ChiTaRS; ARF4; human.
DR EvolutionaryTrace; P18085; -.
DR GeneWiki; ARF4; -.
DR GenomeRNAi; 378; -.
DR NextBio; 1583; -.
DR PRO; PR:P18085; -.
DR ArrayExpress; P18085; -.
DR Bgee; P18085; -.
DR CleanEx; HS_ARF4; -.
DR Genevestigator; P18085; -.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0007420; P:brain development; IEA:Ensembl.
DR GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0048678; P:response to axon injury; IEA:Ensembl.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR024156; Small_GTPase_ARF.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00177; ARF; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; ER-Golgi transport; Golgi apparatus;
KW GTP-binding; Lipoprotein; Myristate; Nucleotide-binding; Polymorphism;
KW Protein transport; Reference proteome; Transport.
FT INIT_MET 1 1 Removed (Potential).
FT CHAIN 2 180 ADP-ribosylation factor 4.
FT /FTId=PRO_0000207391.
FT NP_BIND 24 31 GTP.
FT NP_BIND 67 71 GTP.
FT NP_BIND 126 129 GTP.
FT LIPID 2 2 N-myristoyl glycine (Potential).
FT VARIANT 68 68 V -> A (in dbSNP:rs11550597).
FT /FTId=VAR_048317.
FT HELIX 5 8
FT TURN 9 12
FT STRAND 14 16
FT STRAND 18 25
FT HELIX 30 37
FT STRAND 42 48
FT STRAND 51 57
FT STRAND 59 67
FT STRAND 69 71
FT HELIX 77 80
FT STRAND 83 95
FT HELIX 97 99
FT HELIX 100 112
FT HELIX 114 116
FT STRAND 120 126
FT HELIX 136 143
FT HELIX 145 147
FT STRAND 153 157
FT HELIX 160 162
FT HELIX 166 177
SQ SEQUENCE 180 AA; 20511 MW; 3A43EB1F37A81BD9 CRC64;
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI QEVADELQKM LLVDELRDAV
LLLFANKQDL PNAMAISEMT DKLGLQSLRN RTWYVQATCA TQGTGLYEGL DWLSNELSKR
//
ID ARF4_HUMAN Reviewed; 180 AA.
AC P18085; B2R7J7; P21371;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 3.
DT 22-JAN-2014, entry version 148.
DE RecName: Full=ADP-ribosylation factor 4;
GN Name=ARF4; Synonyms=ARF2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2107548; DOI=10.1073/pnas.87.6.2206;
RA Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J.,
RA Vaughan M.;
RT "Selective amplification of an mRNA and related pseudogene for a human
RT ADP-ribosylation factor, a guanine nucleotide-dependent protein
RT activator of cholera toxin.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:2206-2210(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1899243;
RA Kahn R.A., Kern F.G., Clark J., Gelmann E.P., Rulka C.;
RT "Human ADP-ribosylation factors. A functionally conserved family of
RT GTP-binding proteins.";
RL J. Biol. Chem. 266:2606-2614(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10524252; DOI=10.1016/S0378-1119(99)00290-5;
RA Lebeda R.A., Haun R.S.;
RT "Cloning and characterization of the human ADP-ribosylation factor 4
RT gene.";
RL Gene 237:209-214(1999).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction
RT sequenced by the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow, Placenta, Skin, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "Structure of human ADP-ribosylation factor 4.";
RL Submitted (APR-2005) to the PDB data bank.
CC -!- FUNCTION: GTP-binding protein that functions as an allosteric
CC activator of the cholera toxin catalytic subunit, an ADP-
CC ribosyltransferase. Involved in protein trafficking; may modulate
CC vesicle budding and uncoating within the Golgi apparatus.
CC -!- INTERACTION:
CC Q96PM5:RCHY1; NbExp=3; IntAct=EBI-1237085, EBI-947779;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC -!- CAUTION: Was originally thought to be ARF2.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M36341; AAA53081.1; -; mRNA.
DR EMBL; AF104238; AAD54674.1; -; Genomic_DNA.
DR EMBL; AF104233; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104234; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104235; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104236; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF104237; AAD54674.1; JOINED; Genomic_DNA.
DR EMBL; AF493883; AAM12597.1; -; mRNA.
DR EMBL; AK313008; BAG35844.1; -; mRNA.
DR EMBL; BC003364; AAH03364.1; -; mRNA.
DR EMBL; BC008753; AAH08753.1; -; mRNA.
DR EMBL; BC016325; AAH16325.1; -; mRNA.
DR EMBL; BC022866; AAH22866.1; -; mRNA.
DR PIR; B38622; B38622.
DR RefSeq; NP_001651.1; NM_001660.3.
DR UniGene; Hs.652183; -.
DR PDB; 1Z6X; X-ray; 2.70 A; A/B=2-179.
DR PDBsum; 1Z6X; -.
DR ProteinModelPortal; P18085; -.
DR SMR; P18085; 4-178.
DR IntAct; P18085; 7.
DR MINT; MINT-2801449; -.
DR STRING; 9606.ENSP00000306010; -.
DR PhosphoSite; P18085; -.
DR DMDM; 114123; -.
DR PaxDb; P18085; -.
DR PRIDE; P18085; -.
DR DNASU; 378; -.
DR Ensembl; ENST00000303436; ENSP00000306010; ENSG00000168374.
DR GeneID; 378; -.
DR KEGG; hsa:378; -.
DR UCSC; uc003dix.4; human.
DR CTD; 378; -.
DR GeneCards; GC03M057532; -.
DR HGNC; HGNC:655; ARF4.
DR MIM; 601177; gene.
DR neXtProt; NX_P18085; -.
DR PharmGKB; PA24937; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000163691; -.
DR HOVERGEN; HBG002073; -.
DR InParanoid; P18085; -.
DR KO; K07939; -.
DR OMA; GADELQK; -.
DR OrthoDB; EOG77WWDV; -.
DR PhylomeDB; P18085; -.
DR ChiTaRS; ARF4; human.
DR EvolutionaryTrace; P18085; -.
DR GeneWiki; ARF4; -.
DR GenomeRNAi; 378; -.
DR NextBio; 1583; -.
DR PRO; PR:P18085; -.
DR ArrayExpress; P18085; -.
DR Bgee; P18085; -.
DR CleanEx; HS_ARF4; -.
DR Genevestigator; P18085; -.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:Ensembl.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0007420; P:brain development; IEA:Ensembl.
DR GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0048678; P:response to axon injury; IEA:Ensembl.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR024156; Small_GTPase_ARF.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00177; ARF; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; ER-Golgi transport; Golgi apparatus;
KW GTP-binding; Lipoprotein; Myristate; Nucleotide-binding; Polymorphism;
KW Protein transport; Reference proteome; Transport.
FT INIT_MET 1 1 Removed (Potential).
FT CHAIN 2 180 ADP-ribosylation factor 4.
FT /FTId=PRO_0000207391.
FT NP_BIND 24 31 GTP.
FT NP_BIND 67 71 GTP.
FT NP_BIND 126 129 GTP.
FT LIPID 2 2 N-myristoyl glycine (Potential).
FT VARIANT 68 68 V -> A (in dbSNP:rs11550597).
FT /FTId=VAR_048317.
FT HELIX 5 8
FT TURN 9 12
FT STRAND 14 16
FT STRAND 18 25
FT HELIX 30 37
FT STRAND 42 48
FT STRAND 51 57
FT STRAND 59 67
FT STRAND 69 71
FT HELIX 77 80
FT STRAND 83 95
FT HELIX 97 99
FT HELIX 100 112
FT HELIX 114 116
FT STRAND 120 126
FT HELIX 136 143
FT HELIX 145 147
FT STRAND 153 157
FT HELIX 160 162
FT HELIX 166 177
SQ SEQUENCE 180 AA; 20511 MW; 3A43EB1F37A81BD9 CRC64;
MGLTISSLFS RLFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
ICFTVWDVGG QDRIRPLWKH YFQNTQGLIF VVDSNDRERI QEVADELQKM LLVDELRDAV
LLLFANKQDL PNAMAISEMT DKLGLQSLRN RTWYVQATCA TQGTGLYEGL DWLSNELSKR
//
MIM
601177
*RECORD*
*FIELD* NO
601177
*FIELD* TI
*601177 ADP-RIBOSYLATION FACTOR 4; ARF4
;;ADP-RIBOSYLATION FACTOR 2, FORMERLY; ARF2, FORMERLY
read more*FIELD* TX
CLONING
Monaco et al. (1990) used degenerate PCR with genomic DNA to identify a
member of the human ADP-ribosylation factor (ARF) gene family. The gene,
designated ARF4, encodes a predicted 180-amino acid protein that is
approximately 80% identical to the other human ARFs (see, for example,
103180) and 73% identical to yeast ARF. By DNA sequence analysis, the
authors stated that ARF4 is the most divergent member of the human ARFs.
A processed pseudogene that is 94% identical at the nucleotide level was
also identified. Northern blot analysis detected a 1.8-kb mRNA in IMR-32
neuroblastoma cells.
MAPPING
By fluorescence in situ hybridization, Vorobieva et al. (1995) mapped
the ARF4 gene, which they called ARF2, to chromosome 3p21.2-p21.1.
Kim (1998) mapped the ARF4 gene to 3p14.1 by PCR analysis of somatic
cell hybrid DNA and radiation hybrid analysis.
*FIELD* RF
1. Kim, H.-S.: Human ADP-ribosylation factor 4 (ARF4) gene map position
3p14.1. Chromosome Res. 6: 663 only, 1998.
2. Monaco, L.; Murtagh, J. J.; Newman, K. B.; Tsai, S.-C.; Moss, J.;
Vaughan, M.: Selective amplification of an mRNA and related pseudogene
for a human ADP-ribosylation factor, a guanine nucleotide-dependent
protein activator of cholera toxin. Proc. Nat. Acad. Sci. 87: 2206-2210,
1990.
3. Vorobieva, N.; Protopopov, A.; Protopopova, M.; Kashuba, V.; Allikmets,
R. L.; Modi, W.; Zabarovsky, E. R.; Klein, G.; Kisselev, L.; Graphodatsky,
A.: Localization of human ARF2 and NCK genes and 13 other NotI-linking
clones to chromosome 3 by fluorescence in situ hybridization. Cytogenet.
Cell Genet. 68: 91-94, 1995.
*FIELD* CN
Joanna S. Amberger - updated: 1/22/2004
*FIELD* CD
Alan F. Scott: 4/4/1996
*FIELD* ED
carol: 06/25/2007
carol: 1/23/2004
joanna: 1/22/2004
dkim: 6/26/1998
mark: 4/4/1996
terry: 4/4/1996
mark: 4/4/1996
*RECORD*
*FIELD* NO
601177
*FIELD* TI
*601177 ADP-RIBOSYLATION FACTOR 4; ARF4
;;ADP-RIBOSYLATION FACTOR 2, FORMERLY; ARF2, FORMERLY
read more*FIELD* TX
CLONING
Monaco et al. (1990) used degenerate PCR with genomic DNA to identify a
member of the human ADP-ribosylation factor (ARF) gene family. The gene,
designated ARF4, encodes a predicted 180-amino acid protein that is
approximately 80% identical to the other human ARFs (see, for example,
103180) and 73% identical to yeast ARF. By DNA sequence analysis, the
authors stated that ARF4 is the most divergent member of the human ARFs.
A processed pseudogene that is 94% identical at the nucleotide level was
also identified. Northern blot analysis detected a 1.8-kb mRNA in IMR-32
neuroblastoma cells.
MAPPING
By fluorescence in situ hybridization, Vorobieva et al. (1995) mapped
the ARF4 gene, which they called ARF2, to chromosome 3p21.2-p21.1.
Kim (1998) mapped the ARF4 gene to 3p14.1 by PCR analysis of somatic
cell hybrid DNA and radiation hybrid analysis.
*FIELD* RF
1. Kim, H.-S.: Human ADP-ribosylation factor 4 (ARF4) gene map position
3p14.1. Chromosome Res. 6: 663 only, 1998.
2. Monaco, L.; Murtagh, J. J.; Newman, K. B.; Tsai, S.-C.; Moss, J.;
Vaughan, M.: Selective amplification of an mRNA and related pseudogene
for a human ADP-ribosylation factor, a guanine nucleotide-dependent
protein activator of cholera toxin. Proc. Nat. Acad. Sci. 87: 2206-2210,
1990.
3. Vorobieva, N.; Protopopov, A.; Protopopova, M.; Kashuba, V.; Allikmets,
R. L.; Modi, W.; Zabarovsky, E. R.; Klein, G.; Kisselev, L.; Graphodatsky,
A.: Localization of human ARF2 and NCK genes and 13 other NotI-linking
clones to chromosome 3 by fluorescence in situ hybridization. Cytogenet.
Cell Genet. 68: 91-94, 1995.
*FIELD* CN
Joanna S. Amberger - updated: 1/22/2004
*FIELD* CD
Alan F. Scott: 4/4/1996
*FIELD* ED
carol: 06/25/2007
carol: 1/23/2004
joanna: 1/22/2004
dkim: 6/26/1998
mark: 4/4/1996
terry: 4/4/1996
mark: 4/4/1996