Full text data of ARL8B
ARL8B
(ARL10C, GIE1)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
ADP-ribosylation factor-like protein 8B (ADP-ribosylation factor-like protein 10C; Novel small G protein indispensable for equal chromosome segregation 1)
ADP-ribosylation factor-like protein 8B (ADP-ribosylation factor-like protein 10C; Novel small G protein indispensable for equal chromosome segregation 1)
UniProt
Q9NVJ2
ID ARL8B_HUMAN Reviewed; 186 AA.
AC Q9NVJ2;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 117.
DE RecName: Full=ADP-ribosylation factor-like protein 8B;
DE AltName: Full=ADP-ribosylation factor-like protein 10C;
DE AltName: Full=Novel small G protein indispensable for equal chromosome segregation 1;
GN Name=ARL8B; Synonyms=ARL10C, GIE1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS
RP OF THR-34; 49-MET--ARG-58; TRP-70; 74-GLY--TYR-85 AND ASN-130,
RP SUBCELLULAR LOCATION, AND INTERACTION WITH TUBULIN.
RC TISSUE=Brain;
RX PubMed=15331635; DOI=10.1242/jcs.01347;
RA Okai T., Araki Y., Tada M., Tateno T., Kontani K., Katada T.;
RT "Novel small GTPase subfamily capable of associating with tubulin is
RT required for chromosome segregation.";
RL J. Cell Sci. 117:4705-4715(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryonic carcinoma, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 69-77; 147-155 AND 166-182, AND MASS SPECTROMETRY.
RC TISSUE=B-cell lymphoma;
RA Bienvenut W.V.;
RL Submitted (JUN-2005) to UniProtKB.
RN [6]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION,
RP ACETYLATION AT MET-1, AND MUTAGENESIS OF LEU-2; 5-ILE--PHE-12 AND
RP GLN-75.
RX PubMed=16537643; DOI=10.1242/jcs.02958;
RA Hofmann I., Munro S.;
RT "An N-terminally acetylated Arf-like GTPase is localised to lysosomes
RT and affects their motility.";
RL J. Cell Sci. 119:1494-1503(2006).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
RC TISSUE=Placenta;
RX PubMed=17897319; DOI=10.1111/j.1600-0854.2007.00643.x;
RA Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B.,
RA Schaefer H., Elsaesser H.-P., Mann M., Hasilik A.;
RT "Integral and associated lysosomal membrane proteins.";
RL Traffic 8:1676-1686(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-184 IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "GTP-like conformation of GDP-bound ARL10C GTPase.";
RL Submitted (AUG-2005) to the PDB data bank.
CC -!- FUNCTION: May play a role in lysosome motility (PubMed:16537643).
CC May play a role in chromosome segregation (PubMed:15331635).
CC -!- SUBUNIT: Interacts with tubulin.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane. Lysosome membrane.
CC Cytoplasm, cytoskeleton, spindle. Note=According to
CC PubMed:15331635, it localizes with microtubules at the spindle
CC mid-zone during mitosis.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB118751; BAD23992.1; -; mRNA.
DR EMBL; AK001564; BAA91759.1; -; mRNA.
DR EMBL; CR457264; CAG33545.1; -; mRNA.
DR EMBL; BC013131; AAH13131.1; -; mRNA.
DR EMBL; BC063125; AAH63125.1; -; mRNA.
DR RefSeq; NP_060654.1; NM_018184.2.
DR UniGene; Hs.250009; -.
DR PDB; 2AL7; X-ray; 1.85 A; A=18-184.
DR PDBsum; 2AL7; -.
DR ProteinModelPortal; Q9NVJ2; -.
DR SMR; Q9NVJ2; 18-182.
DR IntAct; Q9NVJ2; 7.
DR MINT; MINT-1429448; -.
DR STRING; 9606.ENSP00000256496; -.
DR PhosphoSite; Q9NVJ2; -.
DR DMDM; 74752996; -.
DR PaxDb; Q9NVJ2; -.
DR PRIDE; Q9NVJ2; -.
DR Ensembl; ENST00000256496; ENSP00000256496; ENSG00000134108.
DR GeneID; 55207; -.
DR KEGG; hsa:55207; -.
DR UCSC; uc003bqg.3; human.
DR CTD; 55207; -.
DR GeneCards; GC03P005163; -.
DR HGNC; HGNC:25564; ARL8B.
DR HPA; HPA038759; -.
DR HPA; HPA040515; -.
DR HPA; HPA045924; -.
DR neXtProt; NX_Q9NVJ2; -.
DR PharmGKB; PA134959531; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000163692; -.
DR HOVERGEN; HBG105214; -.
DR InParanoid; Q9NVJ2; -.
DR KO; K07955; -.
DR OMA; SIQDREI; -.
DR OrthoDB; EOG7F24V2; -.
DR PhylomeDB; Q9NVJ2; -.
DR ChiTaRS; ARL8B; human.
DR EvolutionaryTrace; Q9NVJ2; -.
DR GeneWiki; ARL8B; -.
DR GenomeRNAi; 55207; -.
DR NextBio; 59110; -.
DR PRO; PR:Q9NVJ2; -.
DR ArrayExpress; Q9NVJ2; -.
DR Bgee; Q9NVJ2; -.
DR CleanEx; HS_ARL8B; -.
DR Genevestigator; Q9NVJ2; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030496; C:midbody; IDA:UniProtKB.
DR GO; GO:0051233; C:spindle midzone; IDA:UniProtKB.
DR GO; GO:0043014; F:alpha-tubulin binding; IDA:UniProtKB.
DR GO; GO:0048487; F:beta-tubulin binding; IDA:UniProtKB.
DR GO; GO:0019003; F:GDP binding; IDA:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB.
DR GO; GO:0003924; F:GTPase activity; NAS:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IMP:UniProtKB.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR024156; Small_GTPase_ARF.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00177; ARF; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell cycle; Cell division;
KW Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW Endosome; GTP-binding; Lysosome; Membrane; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1 186 ADP-ribosylation factor-like protein 8B.
FT /FTId=PRO_0000232921.
FT INTRAMEM 1 19 Note=Mediates targeting to membranes.
FT NP_BIND 29 35 GTP.
FT NP_BIND 71 75 GTP (By similarity).
FT NP_BIND 130 133 GTP.
FT MOD_RES 1 1 N-acetylmethionine.
FT MUTAGEN 2 2 L->A: Diffuse cytoplasmic distribution
FT and loss of localization to lysosomes. No
FT effect on acetylation.
FT MUTAGEN 2 2 L->F: No effect on localization and
FT acetylation.
FT MUTAGEN 5 12 ISRLLDWF->ASRALDWA: Diffuse cytoplasmic
FT distribution and loss of localization to
FT lysosomes. No effect on acetylation.
FT MUTAGEN 34 34 T->N: Preferentially binds GDP. Alters
FT chromosome segregation.
FT MUTAGEN 49 58 Missing: Alters chromosome segregation.
FT MUTAGEN 70 70 W->R: Preferentially binds GTP.
FT MUTAGEN 74 85 Missing: Alters chromosome segregation.
FT MUTAGEN 75 75 Q->L: Prevents GTP hydrolysis. No effect
FT on localization. Alters lysosomes
FT cellular distribution and motility.
FT MUTAGEN 130 130 N->I: Loss of GTP/GDP-binding. Affects
FT chromosome segregation.
FT STRAND 19 26
FT HELIX 33 40
FT STRAND 55 62
FT STRAND 65 72
FT HELIX 76 81
FT HELIX 82 86
FT STRAND 90 97
FT HELIX 101 103
FT HELIX 104 115
FT HELIX 118 120
FT STRAND 125 130
FT HELIX 140 146
FT HELIX 149 151
FT STRAND 157 161
FT TURN 164 167
FT HELIX 170 178
SQ SEQUENCE 186 AA; 21539 MW; 5030B01AFD749223 CRC64;
MLALISRLLD WFRSLFWKEE MELTLVGLQY SGKTTFVNVI ASGQFSEDMI PTVGFNMRKV
TKGNVTIKIW DIGGQPRFRS MWERYCRGVN AIVYMIDAAD REKIEASRNE LHNLLDKPQL
QGIPVLVLGN KRDLPNALDE KQLIEKMNLS AIQDREICCY SISCKEKDNI DITLQWLIQH
SKSRRS
//
ID ARL8B_HUMAN Reviewed; 186 AA.
AC Q9NVJ2;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 117.
DE RecName: Full=ADP-ribosylation factor-like protein 8B;
DE AltName: Full=ADP-ribosylation factor-like protein 10C;
DE AltName: Full=Novel small G protein indispensable for equal chromosome segregation 1;
GN Name=ARL8B; Synonyms=ARL10C, GIE1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS
RP OF THR-34; 49-MET--ARG-58; TRP-70; 74-GLY--TYR-85 AND ASN-130,
RP SUBCELLULAR LOCATION, AND INTERACTION WITH TUBULIN.
RC TISSUE=Brain;
RX PubMed=15331635; DOI=10.1242/jcs.01347;
RA Okai T., Araki Y., Tada M., Tateno T., Kontani K., Katada T.;
RT "Novel small GTPase subfamily capable of associating with tubulin is
RT required for chromosome segregation.";
RL J. Cell Sci. 117:4705-4715(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryonic carcinoma, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 69-77; 147-155 AND 166-182, AND MASS SPECTROMETRY.
RC TISSUE=B-cell lymphoma;
RA Bienvenut W.V.;
RL Submitted (JUN-2005) to UniProtKB.
RN [6]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION,
RP ACETYLATION AT MET-1, AND MUTAGENESIS OF LEU-2; 5-ILE--PHE-12 AND
RP GLN-75.
RX PubMed=16537643; DOI=10.1242/jcs.02958;
RA Hofmann I., Munro S.;
RT "An N-terminally acetylated Arf-like GTPase is localised to lysosomes
RT and affects their motility.";
RL J. Cell Sci. 119:1494-1503(2006).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
RC TISSUE=Placenta;
RX PubMed=17897319; DOI=10.1111/j.1600-0854.2007.00643.x;
RA Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B.,
RA Schaefer H., Elsaesser H.-P., Mann M., Hasilik A.;
RT "Integral and associated lysosomal membrane proteins.";
RL Traffic 8:1676-1686(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-184 IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "GTP-like conformation of GDP-bound ARL10C GTPase.";
RL Submitted (AUG-2005) to the PDB data bank.
CC -!- FUNCTION: May play a role in lysosome motility (PubMed:16537643).
CC May play a role in chromosome segregation (PubMed:15331635).
CC -!- SUBUNIT: Interacts with tubulin.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane. Lysosome membrane.
CC Cytoplasm, cytoskeleton, spindle. Note=According to
CC PubMed:15331635, it localizes with microtubules at the spindle
CC mid-zone during mitosis.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Arf family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB118751; BAD23992.1; -; mRNA.
DR EMBL; AK001564; BAA91759.1; -; mRNA.
DR EMBL; CR457264; CAG33545.1; -; mRNA.
DR EMBL; BC013131; AAH13131.1; -; mRNA.
DR EMBL; BC063125; AAH63125.1; -; mRNA.
DR RefSeq; NP_060654.1; NM_018184.2.
DR UniGene; Hs.250009; -.
DR PDB; 2AL7; X-ray; 1.85 A; A=18-184.
DR PDBsum; 2AL7; -.
DR ProteinModelPortal; Q9NVJ2; -.
DR SMR; Q9NVJ2; 18-182.
DR IntAct; Q9NVJ2; 7.
DR MINT; MINT-1429448; -.
DR STRING; 9606.ENSP00000256496; -.
DR PhosphoSite; Q9NVJ2; -.
DR DMDM; 74752996; -.
DR PaxDb; Q9NVJ2; -.
DR PRIDE; Q9NVJ2; -.
DR Ensembl; ENST00000256496; ENSP00000256496; ENSG00000134108.
DR GeneID; 55207; -.
DR KEGG; hsa:55207; -.
DR UCSC; uc003bqg.3; human.
DR CTD; 55207; -.
DR GeneCards; GC03P005163; -.
DR HGNC; HGNC:25564; ARL8B.
DR HPA; HPA038759; -.
DR HPA; HPA040515; -.
DR HPA; HPA045924; -.
DR neXtProt; NX_Q9NVJ2; -.
DR PharmGKB; PA134959531; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000163692; -.
DR HOVERGEN; HBG105214; -.
DR InParanoid; Q9NVJ2; -.
DR KO; K07955; -.
DR OMA; SIQDREI; -.
DR OrthoDB; EOG7F24V2; -.
DR PhylomeDB; Q9NVJ2; -.
DR ChiTaRS; ARL8B; human.
DR EvolutionaryTrace; Q9NVJ2; -.
DR GeneWiki; ARL8B; -.
DR GenomeRNAi; 55207; -.
DR NextBio; 59110; -.
DR PRO; PR:Q9NVJ2; -.
DR ArrayExpress; Q9NVJ2; -.
DR Bgee; Q9NVJ2; -.
DR CleanEx; HS_ARL8B; -.
DR Genevestigator; Q9NVJ2; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030496; C:midbody; IDA:UniProtKB.
DR GO; GO:0051233; C:spindle midzone; IDA:UniProtKB.
DR GO; GO:0043014; F:alpha-tubulin binding; IDA:UniProtKB.
DR GO; GO:0048487; F:beta-tubulin binding; IDA:UniProtKB.
DR GO; GO:0019003; F:GDP binding; IDA:UniProtKB.
DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB.
DR GO; GO:0003924; F:GTPase activity; NAS:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IMP:UniProtKB.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR024156; Small_GTPase_ARF.
DR InterPro; IPR006689; Small_GTPase_ARF/SAR.
DR Pfam; PF00025; Arf; 1.
DR PRINTS; PR00328; SAR1GTPBP.
DR SMART; SM00177; ARF; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51417; ARF; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell cycle; Cell division;
KW Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW Endosome; GTP-binding; Lysosome; Membrane; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1 186 ADP-ribosylation factor-like protein 8B.
FT /FTId=PRO_0000232921.
FT INTRAMEM 1 19 Note=Mediates targeting to membranes.
FT NP_BIND 29 35 GTP.
FT NP_BIND 71 75 GTP (By similarity).
FT NP_BIND 130 133 GTP.
FT MOD_RES 1 1 N-acetylmethionine.
FT MUTAGEN 2 2 L->A: Diffuse cytoplasmic distribution
FT and loss of localization to lysosomes. No
FT effect on acetylation.
FT MUTAGEN 2 2 L->F: No effect on localization and
FT acetylation.
FT MUTAGEN 5 12 ISRLLDWF->ASRALDWA: Diffuse cytoplasmic
FT distribution and loss of localization to
FT lysosomes. No effect on acetylation.
FT MUTAGEN 34 34 T->N: Preferentially binds GDP. Alters
FT chromosome segregation.
FT MUTAGEN 49 58 Missing: Alters chromosome segregation.
FT MUTAGEN 70 70 W->R: Preferentially binds GTP.
FT MUTAGEN 74 85 Missing: Alters chromosome segregation.
FT MUTAGEN 75 75 Q->L: Prevents GTP hydrolysis. No effect
FT on localization. Alters lysosomes
FT cellular distribution and motility.
FT MUTAGEN 130 130 N->I: Loss of GTP/GDP-binding. Affects
FT chromosome segregation.
FT STRAND 19 26
FT HELIX 33 40
FT STRAND 55 62
FT STRAND 65 72
FT HELIX 76 81
FT HELIX 82 86
FT STRAND 90 97
FT HELIX 101 103
FT HELIX 104 115
FT HELIX 118 120
FT STRAND 125 130
FT HELIX 140 146
FT HELIX 149 151
FT STRAND 157 161
FT TURN 164 167
FT HELIX 170 178
SQ SEQUENCE 186 AA; 21539 MW; 5030B01AFD749223 CRC64;
MLALISRLLD WFRSLFWKEE MELTLVGLQY SGKTTFVNVI ASGQFSEDMI PTVGFNMRKV
TKGNVTIKIW DIGGQPRFRS MWERYCRGVN AIVYMIDAAD REKIEASRNE LHNLLDKPQL
QGIPVLVLGN KRDLPNALDE KQLIEKMNLS AIQDREICCY SISCKEKDNI DITLQWLIQH
SKSRRS
//