Full text data of BIN3
BIN3
[Confidence: low (only semi-automatic identification from reviews)]
Bridging integrator 3
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Bridging integrator 3
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q9NQY0
ID BIN3_HUMAN Reviewed; 253 AA.
AC Q9NQY0; Q9BVG2; Q9NVY9;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 91.
DE RecName: Full=Bridging integrator 3;
GN Name=BIN3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=11274158; DOI=10.1074/jbc.M101096200;
RA Routhier E.L., Burn T.C., Abbaszade I., Summers M., Albright C.F.,
RA Prendergast G.C.;
RT "Human BIN3 complements the F-actin localization defects caused by
RT loss of Hob3p, the fission yeast homolog of Rvs161p.";
RL J. Biol. Chem. 276:21670-21677(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Involved in cytokinesis and septation where it has a
CC role in the localization of F-actin.
CC -!- INTERACTION:
CC Q6XZF7:DNMBP; NbExp=2; IntAct=EBI-2653038, EBI-2483419;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NQY0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NQY0-2; Sequence=VSP_013465;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed except in brain.
CC -!- SIMILARITY: Contains 1 BAR domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH01223.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF271732; AAF76218.1; -; mRNA.
DR EMBL; AK001289; BAA91603.1; -; mRNA.
DR EMBL; AK023980; BAB14751.1; -; mRNA.
DR EMBL; BC001223; AAH01223.1; ALT_INIT; mRNA.
DR EMBL; BC009824; AAH09824.1; -; mRNA.
DR RefSeq; NP_061158.1; NM_018688.4.
DR UniGene; Hs.433722; -.
DR ProteinModelPortal; Q9NQY0; -.
DR SMR; Q9NQY0; 24-219.
DR IntAct; Q9NQY0; 1.
DR STRING; 9606.ENSP00000276416; -.
DR PhosphoSite; Q9NQY0; -.
DR DMDM; 30912742; -.
DR PaxDb; Q9NQY0; -.
DR PRIDE; Q9NQY0; -.
DR DNASU; 55909; -.
DR Ensembl; ENST00000276416; ENSP00000276416; ENSG00000147439.
DR Ensembl; ENST00000519513; ENSP00000430423; ENSG00000147439.
DR GeneID; 55909; -.
DR KEGG; hsa:55909; -.
DR UCSC; uc003xcl.3; human.
DR CTD; 55909; -.
DR GeneCards; GC08M022477; -.
DR HGNC; HGNC:1054; BIN3.
DR HPA; HPA023769; -.
DR MIM; 606396; gene.
DR neXtProt; NX_Q9NQY0; -.
DR PharmGKB; PA25357; -.
DR eggNOG; NOG264615; -.
DR HOGENOM; HOG000007254; -.
DR HOVERGEN; HBG001358; -.
DR InParanoid; Q9NQY0; -.
DR OMA; KFAEEGY; -.
DR OrthoDB; EOG78WKSG; -.
DR PhylomeDB; Q9NQY0; -.
DR GeneWiki; BIN3; -.
DR GenomeRNAi; 55909; -.
DR NextBio; 61298; -.
DR PRO; PR:Q9NQY0; -.
DR ArrayExpress; Q9NQY0; -.
DR Bgee; Q9NQY0; -.
DR CleanEx; HS_BIN3; -.
DR Genevestigator; Q9NQY0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0008093; F:cytoskeletal adaptor activity; NAS:UniProtKB.
DR GO; GO:0007015; P:actin filament organization; NAS:UniProtKB.
DR GO; GO:0000917; P:barrier septum assembly; IEA:UniProtKB-KW.
DR GO; GO:0000910; P:cytokinesis; NAS:UniProtKB.
DR GO; GO:0008104; P:protein localization; IMP:UniProtKB.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:UniProtKB.
DR Gene3D; 1.20.1270.60; -; 1.
DR InterPro; IPR027267; AH/BAR-dom.
DR InterPro; IPR004148; BAR_dom.
DR Pfam; PF03114; BAR; 1.
DR SMART; SM00721; BAR; 1.
DR PROSITE; PS51021; BAR; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Coiled coil;
KW Complete proteome; Cytoplasm; Cytoskeleton; Reference proteome;
KW Septation.
FT CHAIN 1 253 Bridging integrator 3.
FT /FTId=PRO_0000192955.
FT DOMAIN 9 232 BAR.
FT COILED 18 51 Potential.
FT COILED 120 152 Potential.
FT COILED 231 247 Potential.
FT VAR_SEQ 1 54 Missing (in isoform 2).
FT /FTId=VSP_013465.
FT CONFLICT 122 122 N -> I (in Ref. 2; BAA91603).
SQ SEQUENCE 253 AA; 29665 MW; 254CC7113749C584 CRC64;
MSWIPFKIGQ PKKQIVPKTV ERDFEREYGK LQQLEEQTRR LQKDMKKSTD ADLAMSKSAV
KISLDLLSNP LCEQDQDLLN MVTALDTAMK RMDAFNQEKV NQIQKTVIEP LKKFGSVFPS
LNMAVKRREQ ALQDYRRLQA KVEKYEEKEK TGPVLAKLHQ AREELRPVRE DFEAKNRQLL
EEMPRFYGSR LDYFQPSFES LIRAQVVYYS EMHKIFGDLS HQLDQPGHSD EQRERENEAK
LSELRALSIV ADD
//
ID BIN3_HUMAN Reviewed; 253 AA.
AC Q9NQY0; Q9BVG2; Q9NVY9;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 91.
DE RecName: Full=Bridging integrator 3;
GN Name=BIN3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RX PubMed=11274158; DOI=10.1074/jbc.M101096200;
RA Routhier E.L., Burn T.C., Abbaszade I., Summers M., Albright C.F.,
RA Prendergast G.C.;
RT "Human BIN3 complements the F-actin localization defects caused by
RT loss of Hob3p, the fission yeast homolog of Rvs161p.";
RL J. Biol. Chem. 276:21670-21677(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Lung, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Involved in cytokinesis and septation where it has a
CC role in the localization of F-actin.
CC -!- INTERACTION:
CC Q6XZF7:DNMBP; NbExp=2; IntAct=EBI-2653038, EBI-2483419;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NQY0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NQY0-2; Sequence=VSP_013465;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed except in brain.
CC -!- SIMILARITY: Contains 1 BAR domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH01223.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF271732; AAF76218.1; -; mRNA.
DR EMBL; AK001289; BAA91603.1; -; mRNA.
DR EMBL; AK023980; BAB14751.1; -; mRNA.
DR EMBL; BC001223; AAH01223.1; ALT_INIT; mRNA.
DR EMBL; BC009824; AAH09824.1; -; mRNA.
DR RefSeq; NP_061158.1; NM_018688.4.
DR UniGene; Hs.433722; -.
DR ProteinModelPortal; Q9NQY0; -.
DR SMR; Q9NQY0; 24-219.
DR IntAct; Q9NQY0; 1.
DR STRING; 9606.ENSP00000276416; -.
DR PhosphoSite; Q9NQY0; -.
DR DMDM; 30912742; -.
DR PaxDb; Q9NQY0; -.
DR PRIDE; Q9NQY0; -.
DR DNASU; 55909; -.
DR Ensembl; ENST00000276416; ENSP00000276416; ENSG00000147439.
DR Ensembl; ENST00000519513; ENSP00000430423; ENSG00000147439.
DR GeneID; 55909; -.
DR KEGG; hsa:55909; -.
DR UCSC; uc003xcl.3; human.
DR CTD; 55909; -.
DR GeneCards; GC08M022477; -.
DR HGNC; HGNC:1054; BIN3.
DR HPA; HPA023769; -.
DR MIM; 606396; gene.
DR neXtProt; NX_Q9NQY0; -.
DR PharmGKB; PA25357; -.
DR eggNOG; NOG264615; -.
DR HOGENOM; HOG000007254; -.
DR HOVERGEN; HBG001358; -.
DR InParanoid; Q9NQY0; -.
DR OMA; KFAEEGY; -.
DR OrthoDB; EOG78WKSG; -.
DR PhylomeDB; Q9NQY0; -.
DR GeneWiki; BIN3; -.
DR GenomeRNAi; 55909; -.
DR NextBio; 61298; -.
DR PRO; PR:Q9NQY0; -.
DR ArrayExpress; Q9NQY0; -.
DR Bgee; Q9NQY0; -.
DR CleanEx; HS_BIN3; -.
DR Genevestigator; Q9NQY0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0008093; F:cytoskeletal adaptor activity; NAS:UniProtKB.
DR GO; GO:0007015; P:actin filament organization; NAS:UniProtKB.
DR GO; GO:0000917; P:barrier septum assembly; IEA:UniProtKB-KW.
DR GO; GO:0000910; P:cytokinesis; NAS:UniProtKB.
DR GO; GO:0008104; P:protein localization; IMP:UniProtKB.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:UniProtKB.
DR Gene3D; 1.20.1270.60; -; 1.
DR InterPro; IPR027267; AH/BAR-dom.
DR InterPro; IPR004148; BAR_dom.
DR Pfam; PF03114; BAR; 1.
DR SMART; SM00721; BAR; 1.
DR PROSITE; PS51021; BAR; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell cycle; Cell division; Coiled coil;
KW Complete proteome; Cytoplasm; Cytoskeleton; Reference proteome;
KW Septation.
FT CHAIN 1 253 Bridging integrator 3.
FT /FTId=PRO_0000192955.
FT DOMAIN 9 232 BAR.
FT COILED 18 51 Potential.
FT COILED 120 152 Potential.
FT COILED 231 247 Potential.
FT VAR_SEQ 1 54 Missing (in isoform 2).
FT /FTId=VSP_013465.
FT CONFLICT 122 122 N -> I (in Ref. 2; BAA91603).
SQ SEQUENCE 253 AA; 29665 MW; 254CC7113749C584 CRC64;
MSWIPFKIGQ PKKQIVPKTV ERDFEREYGK LQQLEEQTRR LQKDMKKSTD ADLAMSKSAV
KISLDLLSNP LCEQDQDLLN MVTALDTAMK RMDAFNQEKV NQIQKTVIEP LKKFGSVFPS
LNMAVKRREQ ALQDYRRLQA KVEKYEEKEK TGPVLAKLHQ AREELRPVRE DFEAKNRQLL
EEMPRFYGSR LDYFQPSFES LIRAQVVYYS EMHKIFGDLS HQLDQPGHSD EQRERENEAK
LSELRALSIV ADD
//
MIM
606396
*RECORD*
*FIELD* NO
606396
*FIELD* TI
*606396 BRIDGING INTEGRATOR 3; BIN3
*FIELD* TX
DESCRIPTION
Bin/amphiphysin/Rvs (BAR) domain adaptor proteins, such as BIN3, are
read morenamed for their unique N-terminal region and appear to integrate signal
transduction pathways that regulate membrane dynamics, F-actin
cytoskeleton, and nuclear processes.
CLONING
Using yeast Rvs161 as a probe in an EST database search, Routhier et al.
(2001) identified a cDNA encoding BIN3. The deduced 253-amino acid
protein, like its yeast counterpart, is composed solely of a BAR domain.
Northern blot analysis revealed expression of a 2.2-kb BIN3 transcript
in all adult and embryonic tissues tested except brain. In contrast, the
BAR family members AMPH (600418) and BIN2 (605936) are expressed
primarily in brain and hematopoietic tissues, respectively. Like BIN3,
BIN1 (601248) is widely expressed; however, BIN1 expression is decreased
in tumor cells, while BIN3 expression is not.
GENE FUNCTION
By functional analysis, Routhier et al. (2001) showed that BIN3 rescued
the actin localization defects of hob3+, a mutant S. pombe homolog of
BIN3, but not the osmosensitivity of mutant Rvs161. These results
suggested that some but not all roles of BIN3 have been preserved during
evolution.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the BIN3
gene to chromosome 8 (TMAP stSG28683).
*FIELD* RF
1. Routhier, E. L.; Burn, T. C.; Abbaszade, I.; Summers, M.; Albright,
C. F.; Prendergast, G. C.: Human BIN3 complements the F-actin localization
defects caused by loss of Hob3p, the fission yeast homolog of Rvs161p. J.
Biol. Chem. 276: 21670-21677, 2001.
*FIELD* CD
Paul J. Converse: 10/17/2001
*FIELD* ED
mgross: 10/18/2001
mgross: 10/17/2001
*RECORD*
*FIELD* NO
606396
*FIELD* TI
*606396 BRIDGING INTEGRATOR 3; BIN3
*FIELD* TX
DESCRIPTION
Bin/amphiphysin/Rvs (BAR) domain adaptor proteins, such as BIN3, are
read morenamed for their unique N-terminal region and appear to integrate signal
transduction pathways that regulate membrane dynamics, F-actin
cytoskeleton, and nuclear processes.
CLONING
Using yeast Rvs161 as a probe in an EST database search, Routhier et al.
(2001) identified a cDNA encoding BIN3. The deduced 253-amino acid
protein, like its yeast counterpart, is composed solely of a BAR domain.
Northern blot analysis revealed expression of a 2.2-kb BIN3 transcript
in all adult and embryonic tissues tested except brain. In contrast, the
BAR family members AMPH (600418) and BIN2 (605936) are expressed
primarily in brain and hematopoietic tissues, respectively. Like BIN3,
BIN1 (601248) is widely expressed; however, BIN1 expression is decreased
in tumor cells, while BIN3 expression is not.
GENE FUNCTION
By functional analysis, Routhier et al. (2001) showed that BIN3 rescued
the actin localization defects of hob3+, a mutant S. pombe homolog of
BIN3, but not the osmosensitivity of mutant Rvs161. These results
suggested that some but not all roles of BIN3 have been preserved during
evolution.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the BIN3
gene to chromosome 8 (TMAP stSG28683).
*FIELD* RF
1. Routhier, E. L.; Burn, T. C.; Abbaszade, I.; Summers, M.; Albright,
C. F.; Prendergast, G. C.: Human BIN3 complements the F-actin localization
defects caused by loss of Hob3p, the fission yeast homolog of Rvs161p. J.
Biol. Chem. 276: 21670-21677, 2001.
*FIELD* CD
Paul J. Converse: 10/17/2001
*FIELD* ED
mgross: 10/18/2001
mgross: 10/17/2001