Full text data of CLTB
CLTB
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Clathrin light chain B; Lcb
Clathrin light chain B; Lcb
UniProt
P09497
ID CLCB_HUMAN Reviewed; 229 AA.
AC P09497; Q53Y37; Q6FHW1;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUL-1989, sequence version 1.
DT 22-JAN-2014, entry version 124.
DE RecName: Full=Clathrin light chain B;
DE Short=Lcb;
GN Name=CLTB;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RX PubMed=3267234;
RA Jackson A.P., Parham P.;
RT "Structure of human clathrin light chains. Conservation of light chain
RT polymorphism in three mammalian species.";
RL J. Biol. Chem. 263:16688-16695(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
RA Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
RA Korn B., Zuo D., Hu Y., LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
RC TISSUE=Lung, and Retinal pigment epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP INTERACTION WITH HIP1 AND HIP1R.
RX PubMed=11889126; DOI=10.1074/jbc.M112310200;
RA Legendre-Guillemin V., Metzler M., Charbonneau M., Gan L., Chopra V.,
RA Philie J., Hayden M.R., McPherson P.S.;
RT "HIP1 and HIP12 display differential binding to F-actin, AP2, and
RT clathrin. Identification of a novel interaction with clathrin light
RT chain.";
RL J. Biol. Chem. 277:19897-19904(2002).
RN [7]
RP INTERACTION WITH HIP1R.
RX PubMed=15533940; DOI=10.1074/jbc.M408454200;
RA Chen C.-Y., Brodsky F.M.;
RT "Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein
RT (Hip1R) bind the conserved sequence of clathrin light chains and
RT thereby influence clathrin assembly in vitro and actin distribution in
RT vivo.";
RL J. Biol. Chem. 280:6109-6117(2005).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Clathrin is the major protein of the polyhedral coat of
CC coated pits and vesicles.
CC -!- SUBUNIT: Clathrin coats are formed from molecules containing 3
CC heavy chains and 3 light chains. Interacts (via N-terminus) with
CC HIP1. Interacts with HIP1R.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Peripheral
CC membrane protein; Cytoplasmic side. Membrane, coated pit;
CC Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic
CC face of coated pits and vesicles.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Brain;
CC IsoId=P09497-1; Sequence=Displayed;
CC Name=Non-brain;
CC IsoId=P09497-2; Sequence=VSP_001098;
CC -!- SIMILARITY: Belongs to the clathrin light chain family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M20469; AAA51818.1; -; mRNA.
DR EMBL; M20470; AAA59506.1; -; mRNA.
DR EMBL; BT007028; AAP35675.1; -; mRNA.
DR EMBL; CR536577; CAG38814.1; -; mRNA.
DR EMBL; CH471195; EAW85079.1; -; Genomic_DNA.
DR EMBL; BC006332; AAH06332.1; -; mRNA.
DR EMBL; BC006457; AAH06457.1; -; mRNA.
DR PIR; B31775; B31775.
DR RefSeq; NP_001825.1; NM_001834.3.
DR RefSeq; NP_009028.1; NM_007097.3.
DR UniGene; Hs.484241; -.
DR ProteinModelPortal; P09497; -.
DR IntAct; P09497; 3.
DR MINT; MINT-1390805; -.
DR STRING; 9606.ENSP00000309415; -.
DR PhosphoSite; P09497; -.
DR DMDM; 116505; -.
DR OGP; P09497; -.
DR PaxDb; P09497; -.
DR PRIDE; P09497; -.
DR DNASU; 1212; -.
DR Ensembl; ENST00000310418; ENSP00000309415; ENSG00000175416.
DR Ensembl; ENST00000345807; ENSP00000310812; ENSG00000175416.
DR GeneID; 1212; -.
DR KEGG; hsa:1212; -.
DR UCSC; uc003meh.4; human.
DR CTD; 1212; -.
DR GeneCards; GC05M175819; -.
DR HGNC; HGNC:2091; CLTB.
DR HPA; CAB003692; -.
DR MIM; 118970; gene.
DR neXtProt; NX_P09497; -.
DR PharmGKB; PA26617; -.
DR eggNOG; NOG312397; -.
DR HOGENOM; HOG000008147; -.
DR HOVERGEN; HBG003386; -.
DR InParanoid; P09497; -.
DR KO; K04645; -.
DR OMA; VMEQEWR; -.
DR OrthoDB; EOG79KPH4; -.
DR PhylomeDB; P09497; -.
DR Reactome; REACT_11123; Membrane Trafficking.
DR ChiTaRS; CLTB; human.
DR GenomeRNAi; 1212; -.
DR NextBio; 4993; -.
DR PMAP-CutDB; P09497; -.
DR PRO; PR:P09497; -.
DR ArrayExpress; P09497; -.
DR Bgee; P09497; -.
DR CleanEx; HS_CLTB; -.
DR Genevestigator; P09497; -.
DR GO; GO:0060170; C:cilium membrane; IEA:Ensembl.
DR GO; GO:0030118; C:clathrin coat; ISS:UniProtKB.
DR GO; GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
DR GO; GO:0030130; C:clathrin coat of trans-Golgi network vesicle; IEA:InterPro.
DR GO; GO:0005802; C:trans-Golgi network; IEA:Ensembl.
DR GO; GO:0042277; F:peptide binding; IEA:Ensembl.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; NAS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR InterPro; IPR000996; Clathrin_L-chain.
DR PANTHER; PTHR10639; PTHR10639; 1.
DR Pfam; PF01086; Clathrin_lg_ch; 1.
DR PROSITE; PS00224; CLATHRIN_LIGHT_CHN_1; 1.
DR PROSITE; PS00581; CLATHRIN_LIGHT_CHN_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Coated pit; Complete proteome;
KW Cytoplasmic vesicle; Disulfide bond; Membrane; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1 229 Clathrin light chain B.
FT /FTId=PRO_0000205771.
FT REGION 93 155 Involved in binding clathrin heavy chain.
FT MOD_RES 11 11 Phosphoserine (By similarity).
FT MOD_RES 13 13 Phosphoserine (By similarity).
FT DISULFID 199 209 By similarity.
FT VAR_SEQ 156 173 Missing (in isoform Non-brain).
FT /FTId=VSP_001098.
FT CONFLICT 5 5 F -> I (in Ref. 3; CAG38814).
SQ SEQUENCE 229 AA; 25190 MW; 07DBA19D44648F6F CRC64;
MADDFGFFSS SESGAPEAAE EDPAAAFLAQ QESEIAGIEN DEGFGAPAGS HAAPAQPGPT
SGAGSEDMGT TVNGDVFQEA NGPADGYAAI AQADRLTQEP ESIRKWREEQ RKRLQELDAA
SKVTEQEWRE KAKKDLEEWN QRQSEQVEKN KINNRIADKA FYQQPDADII GYVASEEAFV
KESKEETPGT EWEKVAQLCD FNPKSSKQCK DVSRLRSVLM SLKQTPLSR
//
ID CLCB_HUMAN Reviewed; 229 AA.
AC P09497; Q53Y37; Q6FHW1;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUL-1989, sequence version 1.
DT 22-JAN-2014, entry version 124.
DE RecName: Full=Clathrin light chain B;
DE Short=Lcb;
GN Name=CLTB;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RX PubMed=3267234;
RA Jackson A.P., Parham P.;
RT "Structure of human clathrin light chains. Conservation of light chain
RT polymorphism in three mammalian species.";
RL J. Biol. Chem. 263:16688-16695(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
RA Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
RA Korn B., Zuo D., Hu Y., LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
RC TISSUE=Lung, and Retinal pigment epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP INTERACTION WITH HIP1 AND HIP1R.
RX PubMed=11889126; DOI=10.1074/jbc.M112310200;
RA Legendre-Guillemin V., Metzler M., Charbonneau M., Gan L., Chopra V.,
RA Philie J., Hayden M.R., McPherson P.S.;
RT "HIP1 and HIP12 display differential binding to F-actin, AP2, and
RT clathrin. Identification of a novel interaction with clathrin light
RT chain.";
RL J. Biol. Chem. 277:19897-19904(2002).
RN [7]
RP INTERACTION WITH HIP1R.
RX PubMed=15533940; DOI=10.1074/jbc.M408454200;
RA Chen C.-Y., Brodsky F.M.;
RT "Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein
RT (Hip1R) bind the conserved sequence of clathrin light chains and
RT thereby influence clathrin assembly in vitro and actin distribution in
RT vivo.";
RL J. Biol. Chem. 280:6109-6117(2005).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Clathrin is the major protein of the polyhedral coat of
CC coated pits and vesicles.
CC -!- SUBUNIT: Clathrin coats are formed from molecules containing 3
CC heavy chains and 3 light chains. Interacts (via N-terminus) with
CC HIP1. Interacts with HIP1R.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Peripheral
CC membrane protein; Cytoplasmic side. Membrane, coated pit;
CC Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic
CC face of coated pits and vesicles.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Brain;
CC IsoId=P09497-1; Sequence=Displayed;
CC Name=Non-brain;
CC IsoId=P09497-2; Sequence=VSP_001098;
CC -!- SIMILARITY: Belongs to the clathrin light chain family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M20469; AAA51818.1; -; mRNA.
DR EMBL; M20470; AAA59506.1; -; mRNA.
DR EMBL; BT007028; AAP35675.1; -; mRNA.
DR EMBL; CR536577; CAG38814.1; -; mRNA.
DR EMBL; CH471195; EAW85079.1; -; Genomic_DNA.
DR EMBL; BC006332; AAH06332.1; -; mRNA.
DR EMBL; BC006457; AAH06457.1; -; mRNA.
DR PIR; B31775; B31775.
DR RefSeq; NP_001825.1; NM_001834.3.
DR RefSeq; NP_009028.1; NM_007097.3.
DR UniGene; Hs.484241; -.
DR ProteinModelPortal; P09497; -.
DR IntAct; P09497; 3.
DR MINT; MINT-1390805; -.
DR STRING; 9606.ENSP00000309415; -.
DR PhosphoSite; P09497; -.
DR DMDM; 116505; -.
DR OGP; P09497; -.
DR PaxDb; P09497; -.
DR PRIDE; P09497; -.
DR DNASU; 1212; -.
DR Ensembl; ENST00000310418; ENSP00000309415; ENSG00000175416.
DR Ensembl; ENST00000345807; ENSP00000310812; ENSG00000175416.
DR GeneID; 1212; -.
DR KEGG; hsa:1212; -.
DR UCSC; uc003meh.4; human.
DR CTD; 1212; -.
DR GeneCards; GC05M175819; -.
DR HGNC; HGNC:2091; CLTB.
DR HPA; CAB003692; -.
DR MIM; 118970; gene.
DR neXtProt; NX_P09497; -.
DR PharmGKB; PA26617; -.
DR eggNOG; NOG312397; -.
DR HOGENOM; HOG000008147; -.
DR HOVERGEN; HBG003386; -.
DR InParanoid; P09497; -.
DR KO; K04645; -.
DR OMA; VMEQEWR; -.
DR OrthoDB; EOG79KPH4; -.
DR PhylomeDB; P09497; -.
DR Reactome; REACT_11123; Membrane Trafficking.
DR ChiTaRS; CLTB; human.
DR GenomeRNAi; 1212; -.
DR NextBio; 4993; -.
DR PMAP-CutDB; P09497; -.
DR PRO; PR:P09497; -.
DR ArrayExpress; P09497; -.
DR Bgee; P09497; -.
DR CleanEx; HS_CLTB; -.
DR Genevestigator; P09497; -.
DR GO; GO:0060170; C:cilium membrane; IEA:Ensembl.
DR GO; GO:0030118; C:clathrin coat; ISS:UniProtKB.
DR GO; GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
DR GO; GO:0030130; C:clathrin coat of trans-Golgi network vesicle; IEA:InterPro.
DR GO; GO:0005802; C:trans-Golgi network; IEA:Ensembl.
DR GO; GO:0042277; F:peptide binding; IEA:Ensembl.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; NAS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR InterPro; IPR000996; Clathrin_L-chain.
DR PANTHER; PTHR10639; PTHR10639; 1.
DR Pfam; PF01086; Clathrin_lg_ch; 1.
DR PROSITE; PS00224; CLATHRIN_LIGHT_CHN_1; 1.
DR PROSITE; PS00581; CLATHRIN_LIGHT_CHN_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Coated pit; Complete proteome;
KW Cytoplasmic vesicle; Disulfide bond; Membrane; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1 229 Clathrin light chain B.
FT /FTId=PRO_0000205771.
FT REGION 93 155 Involved in binding clathrin heavy chain.
FT MOD_RES 11 11 Phosphoserine (By similarity).
FT MOD_RES 13 13 Phosphoserine (By similarity).
FT DISULFID 199 209 By similarity.
FT VAR_SEQ 156 173 Missing (in isoform Non-brain).
FT /FTId=VSP_001098.
FT CONFLICT 5 5 F -> I (in Ref. 3; CAG38814).
SQ SEQUENCE 229 AA; 25190 MW; 07DBA19D44648F6F CRC64;
MADDFGFFSS SESGAPEAAE EDPAAAFLAQ QESEIAGIEN DEGFGAPAGS HAAPAQPGPT
SGAGSEDMGT TVNGDVFQEA NGPADGYAAI AQADRLTQEP ESIRKWREEQ RKRLQELDAA
SKVTEQEWRE KAKKDLEEWN QRQSEQVEKN KINNRIADKA FYQQPDADII GYVASEEAFV
KESKEETPGT EWEKVAQLCD FNPKSSKQCK DVSRLRSVLM SLKQTPLSR
//
MIM
118970
*RECORD*
*FIELD* NO
118970
*FIELD* TI
*118970 CLATHRIN, LIGHT POLYPEPTIDE B; CLTB
;;LCB
*FIELD* TX
DESCRIPTION
Clathrin is the main structural component of the lattice covering the
read morecytoplasmic face of the coated pits and coated vesicles in which
specific macromolecules are entrapped in the process of
receptor-mediated endocytosis. Clathrin is a large, soluble protein
composed of heavy chains, which have molecular masses of about 192 kD,
and light chains, which have molecular masses of about 32 to 38 kD. Two
major classes of clathrin light chains, referred to as LCA (CLTA;
118960) and LCB (CTLB), have been identified (summary by Kirchhausen et
al., 1987). For additional information on clathrin, see 118960.
CLONING
Jackson et al. (1987) identified 4 distinct forms of bovine clathrin
light chains. This molecular variability derived from tissue-specific
splicing of Lca and Lcb genes.
Brodsky et al. (1987) identified that part of the light chain sequence
that mediates heavy chain binding and is the region of strongest
homology with intermediate filament proteins. Sequence analysis shows an
overall homology of 60% between LCA and LCB and the presence of
brain-specific insertion sequences.
Jackson and Parham (1988) compared cDNAs encoding the brain and nonbrain
forms of human LCA and LCB with their homologs in cow and rat. The
significant differences that distinguish LCA from LCB and the brain from
the nonbrain forms show remarkable preservation in all 3 species. Each
clathrin triskelion consists of 3 heavy chains and 3 light chains. In
the brain, tissue-specific mRNA splicing yields larger forms of LCA and
LCB, containing additional insertion sequences of 30 and 18 amino acids,
respectively.
MAPPING
By Southern blot analysis carried out on genomic DNA extracted from a
panel of mouse-human somatic cell hybrids and by isotopic in situ
hybridization, Ponnambalam et al. (1994) assigned the CLTB gene to human
chromosome 4q2-q3. However, Gross (2011) mapped the CLTB gene to
chromosome 5q35.2 based on an alignment of the CLTB sequence (GenBank
GENBANK BC006457) with the genomic sequence (GRCh37).
*FIELD* RF
1. Brodsky, F. M.; Galloway, C. J.; Blank, G. S.; Jackson, A. P.;
Seow, H.-F.; Drickamer, K.; Parham, P.: Localization of clathrin
light-chain sequences mediating heavy-chain binding and coated vesicle
diversity. Nature 326: 203-205, 1987.
2. Gross, M. B.: Personal Communication. Baltimore, Md. 4/20/2011.
3. Jackson, A. P.; Parham, P.: Structure of human clathrin light
chains: conservation of light chain polymorphism in three mammalian
species. J. Biol. Chem. 263: 16688-16695, 1988.
4. Jackson, A. P.; Seow, H.-F.; Holmes, N.; Drickamer, K.; Parham,
P.: Clathrin light chains contain brain-specific insertion sequences
and a region of homology with intermediate filaments. Nature 326:
154-159, 1987.
5. Kirchhausen, T.; Scarmato, P.; Harrison, S. C.; Monroe, J. J.;
Chow, E. P.; Mattaliano, R. J.; Ramachandran, K. L.; Smart, J. E.;
Ahn, A. H.; Brosius, J.: Clathrin light chains LCA and LCB are similar,
polymorphic, and share repeated heptad motifs. Science 236: 320-324,
1987.
6. Ponnambalam, S.; Jackson, A. P.; LeBeau, M. M.; Pravtcheva, D.;
Ruddle, F. H.; Alibert, C.; Parham, P.: Chromosomal location and
some structural features of human clathrin light-chain genes (CLTA
and CLTB). Genomics 24: 440-444, 1994.
*FIELD* CN
Matthew B. Gross - updated: 04/20/2011
*FIELD* CD
Victor A. McKusick: 7/7/1987
*FIELD* ED
mgross: 04/20/2011
terry: 4/23/1999
carol: 1/18/1995
supermim: 3/16/1992
supermim: 3/20/1990
ddp: 10/26/1989
marie: 3/25/1988
carol: 7/7/1987
*RECORD*
*FIELD* NO
118970
*FIELD* TI
*118970 CLATHRIN, LIGHT POLYPEPTIDE B; CLTB
;;LCB
*FIELD* TX
DESCRIPTION
Clathrin is the main structural component of the lattice covering the
read morecytoplasmic face of the coated pits and coated vesicles in which
specific macromolecules are entrapped in the process of
receptor-mediated endocytosis. Clathrin is a large, soluble protein
composed of heavy chains, which have molecular masses of about 192 kD,
and light chains, which have molecular masses of about 32 to 38 kD. Two
major classes of clathrin light chains, referred to as LCA (CLTA;
118960) and LCB (CTLB), have been identified (summary by Kirchhausen et
al., 1987). For additional information on clathrin, see 118960.
CLONING
Jackson et al. (1987) identified 4 distinct forms of bovine clathrin
light chains. This molecular variability derived from tissue-specific
splicing of Lca and Lcb genes.
Brodsky et al. (1987) identified that part of the light chain sequence
that mediates heavy chain binding and is the region of strongest
homology with intermediate filament proteins. Sequence analysis shows an
overall homology of 60% between LCA and LCB and the presence of
brain-specific insertion sequences.
Jackson and Parham (1988) compared cDNAs encoding the brain and nonbrain
forms of human LCA and LCB with their homologs in cow and rat. The
significant differences that distinguish LCA from LCB and the brain from
the nonbrain forms show remarkable preservation in all 3 species. Each
clathrin triskelion consists of 3 heavy chains and 3 light chains. In
the brain, tissue-specific mRNA splicing yields larger forms of LCA and
LCB, containing additional insertion sequences of 30 and 18 amino acids,
respectively.
MAPPING
By Southern blot analysis carried out on genomic DNA extracted from a
panel of mouse-human somatic cell hybrids and by isotopic in situ
hybridization, Ponnambalam et al. (1994) assigned the CLTB gene to human
chromosome 4q2-q3. However, Gross (2011) mapped the CLTB gene to
chromosome 5q35.2 based on an alignment of the CLTB sequence (GenBank
GENBANK BC006457) with the genomic sequence (GRCh37).
*FIELD* RF
1. Brodsky, F. M.; Galloway, C. J.; Blank, G. S.; Jackson, A. P.;
Seow, H.-F.; Drickamer, K.; Parham, P.: Localization of clathrin
light-chain sequences mediating heavy-chain binding and coated vesicle
diversity. Nature 326: 203-205, 1987.
2. Gross, M. B.: Personal Communication. Baltimore, Md. 4/20/2011.
3. Jackson, A. P.; Parham, P.: Structure of human clathrin light
chains: conservation of light chain polymorphism in three mammalian
species. J. Biol. Chem. 263: 16688-16695, 1988.
4. Jackson, A. P.; Seow, H.-F.; Holmes, N.; Drickamer, K.; Parham,
P.: Clathrin light chains contain brain-specific insertion sequences
and a region of homology with intermediate filaments. Nature 326:
154-159, 1987.
5. Kirchhausen, T.; Scarmato, P.; Harrison, S. C.; Monroe, J. J.;
Chow, E. P.; Mattaliano, R. J.; Ramachandran, K. L.; Smart, J. E.;
Ahn, A. H.; Brosius, J.: Clathrin light chains LCA and LCB are similar,
polymorphic, and share repeated heptad motifs. Science 236: 320-324,
1987.
6. Ponnambalam, S.; Jackson, A. P.; LeBeau, M. M.; Pravtcheva, D.;
Ruddle, F. H.; Alibert, C.; Parham, P.: Chromosomal location and
some structural features of human clathrin light-chain genes (CLTA
and CLTB). Genomics 24: 440-444, 1994.
*FIELD* CN
Matthew B. Gross - updated: 04/20/2011
*FIELD* CD
Victor A. McKusick: 7/7/1987
*FIELD* ED
mgross: 04/20/2011
terry: 4/23/1999
carol: 1/18/1995
supermim: 3/16/1992
supermim: 3/20/1990
ddp: 10/26/1989
marie: 3/25/1988
carol: 7/7/1987