Full text data of CLIC3
CLIC3
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Chloride intracellular channel protein 3
Chloride intracellular channel protein 3
hRBCD
IPI00000692
IPI00000692 Chloride intracellular channel protein 3 Chloride intracellular channel protein 3 membrane n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 1 n/a n/a integral membrane protein n/a expected molecular weight found in band between 98-188 kDa
IPI00000692 Chloride intracellular channel protein 3 Chloride intracellular channel protein 3 membrane n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 1 n/a n/a integral membrane protein n/a expected molecular weight found in band between 98-188 kDa
UniProt
O95833
ID CLIC3_HUMAN Reviewed; 236 AA.
AC O95833; Q5SPZ7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
read moreDT 13-APR-2004, sequence version 2.
DT 22-JAN-2014, entry version 126.
DE RecName: Full=Chloride intracellular channel protein 3;
GN Name=CLIC3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E.,
RA Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S.,
RA Babbage A.K., Babbage S., Bagguley C.L., Bailey J., Banerjee R.,
RA Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W.,
RA Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G.,
RA Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M.,
RA Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W.,
RA Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A.,
RA Frankland J.A., French L., Fricker D.G., Garner P., Garnett J.,
RA Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C.,
RA Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M.,
RA Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S.,
RA McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J.,
RA Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R.,
RA Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M.,
RA Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M.,
RA Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A.,
RA Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W.,
RA Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S.,
RA Rogers J., Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 5-236, SUBCELLULAR LOCATION, FUNCTION,
RP AND TISSUE SPECIFICITY.
RC TISSUE=Fetal brain;
RX PubMed=9880541; DOI=10.1074/jbc.274.3.1621;
RA Qian Z., Okuhara D., Abe M.K., Rosner M.R.;
RT "Molecular cloning and characterization of a mitogen-activated protein
RT kinase-associated intracellular chloride channel.";
RL J. Biol. Chem. 274:1621-1627(1999).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17027078; DOI=10.1016/j.placenta.2006.08.002;
RA Money T.T., King R.G., Wong M.H., Stevenson J.L., Kalionis B.,
RA Erwich J.J.H.M., Huisman M.A., Timmer A., Hiden U., Desoye G.,
RA Gude N.M.;
RT "Expression and cellular localisation of chloride intracellular
RT channel 3 in human placenta and fetal membranes.";
RL Placenta 28:429-436(2007).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-230, AND DISULFIDE BOND.
RX PubMed=20146363; DOI=10.1002/prot.22675;
RA Littler D.R., Brown L.J., Breit S.N., Perrakis A., Curmi P.M.;
RT "Structure of human CLIC3 at 2 A resolution.";
RL Proteins 78:1594-1600(2010).
CC -!- FUNCTION: Can insert into membranes and form chloride ion
CC channels. May participate in cellular growth control.
CC -!- SUBUNIT: Associated with the C-terminal of ERK7.
CC -!- SUBCELLULAR LOCATION: Nucleus. Membrane; Single-pass membrane
CC protein. Cytoplasm. Note=Predominantly nuclear. Some protein was
CC found in the cytoplasm. Exists both as soluble cytoplasmic protein
CC and as membrane protein with probably a single transmembrane
CC domain (By similarity).
CC -!- TISSUE SPECIFICITY: Detected in placenta (at protein level).
CC Widely expressed. High expression is found in placenta followed by
CC lung and heart. Low expression in skeletal muscle, kidney and
CC pancreas.
CC -!- DOMAIN: Members of this family may change from a globular, soluble
CC state to a state where the N-terminal domain is inserted into the
CC membrane and functions as chloride channel. A conformation change
CC of the N-terminal domain is thought to expose hydrophobic surfaces
CC that trigger membrane insertion (By similarity).
CC -!- SIMILARITY: Belongs to the chloride channel CLIC family.
CC -!- SIMILARITY: Contains 1 GST C-terminal domain.
CC -!- SIMILARITY: Contains 1 GST N-terminal domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD16450.1; Type=Frameshift; Positions=28;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AL807752; CAI12764.1; -; Genomic_DNA.
DR EMBL; CH471090; EAW88329.1; -; Genomic_DNA.
DR EMBL; BC007012; AAH07012.2; -; mRNA.
DR EMBL; AF102166; AAD16450.1; ALT_FRAME; mRNA.
DR RefSeq; NP_004660.2; NM_004669.2.
DR UniGene; Hs.64746; -.
DR PDB; 3FY7; X-ray; 1.95 A; A/B=1-230.
DR PDB; 3KJY; X-ray; 1.95 A; A/B=1-230.
DR PDBsum; 3FY7; -.
DR PDBsum; 3KJY; -.
DR ProteinModelPortal; O95833; -.
DR SMR; O95833; 3-230.
DR STRING; 9606.ENSP00000360678; -.
DR TCDB; 1.A.12.1.7; the intracellular chloride channel (clic) family.
DR PhosphoSite; O95833; -.
DR PaxDb; O95833; -.
DR PeptideAtlas; O95833; -.
DR PRIDE; O95833; -.
DR DNASU; 9022; -.
DR Ensembl; ENST00000494426; ENSP00000419378; ENSG00000169583.
DR GeneID; 9022; -.
DR KEGG; hsa:9022; -.
DR UCSC; uc004ckj.1; human.
DR CTD; 9022; -.
DR GeneCards; GC09M139889; -.
DR HGNC; HGNC:2064; CLIC3.
DR HPA; HPA005963; -.
DR MIM; 606533; gene.
DR neXtProt; NX_O95833; -.
DR PharmGKB; PA26590; -.
DR eggNOG; NOG325572; -.
DR HOGENOM; HOG000231548; -.
DR HOVERGEN; HBG050994; -.
DR InParanoid; O95833; -.
DR KO; K05023; -.
DR OMA; PSCQRLF; -.
DR OrthoDB; EOG7X3QR3; -.
DR PhylomeDB; O95833; -.
DR EvolutionaryTrace; O95833; -.
DR GeneWiki; CLIC3; -.
DR GenomeRNAi; 9022; -.
DR NextBio; 33801; -.
DR PRO; PR:O95833; -.
DR ArrayExpress; O95833; -.
DR Bgee; O95833; -.
DR CleanEx; HS_CLIC3; -.
DR Genevestigator; O95833; -.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005254; F:chloride channel activity; IMP:UniProtKB.
DR GO; GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR Gene3D; 1.20.1050.10; -; 1.
DR Gene3D; 3.40.30.10; -; 1.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR002946; Int_Cl_channel.
DR InterPro; IPR012336; Thioredoxin-like_fold.
DR PANTHER; PTHR11260:SF1; PTHR11260:SF1; 1.
DR Pfam; PF13417; GST_N_3; 1.
DR PRINTS; PR01263; INTCLCHANNEL.
DR SUPFAM; SSF47616; SSF47616; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloride; Chloride channel; Complete proteome;
KW Cytoplasm; Disulfide bond; Ion channel; Ion transport; Membrane;
KW Nucleus; Polymorphism; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1 236 Chloride intracellular channel protein 3.
FT /FTId=PRO_0000144207.
FT TRANSMEM 24 44 Helical; (Potential).
FT DOMAIN 12 90 GST N-terminal.
FT DOMAIN 91 235 GST C-terminal.
FT REGION 1 88 Required for insertion into the membrane
FT (By similarity).
FT DISULFID 22 25 In soluble form.
FT VARIANT 38 38 P -> H (in dbSNP:rs2292923).
FT /FTId=VAR_020424.
FT STRAND 6 12
FT STRAND 16 19
FT HELIX 23 35
FT STRAND 40 44
FT STRAND 64 67
FT STRAND 70 72
FT HELIX 75 85
FT TURN 88 90
FT HELIX 99 103
FT TURN 104 107
FT HELIX 108 117
FT HELIX 121 123
FT HELIX 124 143
FT HELIX 147 152
FT STRAND 162 168
FT HELIX 171 191
FT HELIX 200 210
FT HELIX 213 216
FT HELIX 222 229
SQ SEQUENCE 236 AA; 26648 MW; CA20E66195950886 CRC64;
MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP DVLKDFAPGS
QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE SNTAGNDVFH KFSAFIKNPV
PAQDEALYQQ LLRALARLDS YLRAPLEHEL AGEPQLRESR RRFLDGDRLT LADCSLLPKL
HIVDTVCAHF RQAPIPAELR GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR
//
ID CLIC3_HUMAN Reviewed; 236 AA.
AC O95833; Q5SPZ7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
read moreDT 13-APR-2004, sequence version 2.
DT 22-JAN-2014, entry version 126.
DE RecName: Full=Chloride intracellular channel protein 3;
GN Name=CLIC3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E.,
RA Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S.,
RA Babbage A.K., Babbage S., Bagguley C.L., Bailey J., Banerjee R.,
RA Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W.,
RA Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G.,
RA Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M.,
RA Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W.,
RA Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A.,
RA Frankland J.A., French L., Fricker D.G., Garner P., Garnett J.,
RA Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C.,
RA Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M.,
RA Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S.,
RA McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J.,
RA Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R.,
RA Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M.,
RA Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M.,
RA Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A.,
RA Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W.,
RA Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S.,
RA Rogers J., Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 5-236, SUBCELLULAR LOCATION, FUNCTION,
RP AND TISSUE SPECIFICITY.
RC TISSUE=Fetal brain;
RX PubMed=9880541; DOI=10.1074/jbc.274.3.1621;
RA Qian Z., Okuhara D., Abe M.K., Rosner M.R.;
RT "Molecular cloning and characterization of a mitogen-activated protein
RT kinase-associated intracellular chloride channel.";
RL J. Biol. Chem. 274:1621-1627(1999).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=17027078; DOI=10.1016/j.placenta.2006.08.002;
RA Money T.T., King R.G., Wong M.H., Stevenson J.L., Kalionis B.,
RA Erwich J.J.H.M., Huisman M.A., Timmer A., Hiden U., Desoye G.,
RA Gude N.M.;
RT "Expression and cellular localisation of chloride intracellular
RT channel 3 in human placenta and fetal membranes.";
RL Placenta 28:429-436(2007).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-230, AND DISULFIDE BOND.
RX PubMed=20146363; DOI=10.1002/prot.22675;
RA Littler D.R., Brown L.J., Breit S.N., Perrakis A., Curmi P.M.;
RT "Structure of human CLIC3 at 2 A resolution.";
RL Proteins 78:1594-1600(2010).
CC -!- FUNCTION: Can insert into membranes and form chloride ion
CC channels. May participate in cellular growth control.
CC -!- SUBUNIT: Associated with the C-terminal of ERK7.
CC -!- SUBCELLULAR LOCATION: Nucleus. Membrane; Single-pass membrane
CC protein. Cytoplasm. Note=Predominantly nuclear. Some protein was
CC found in the cytoplasm. Exists both as soluble cytoplasmic protein
CC and as membrane protein with probably a single transmembrane
CC domain (By similarity).
CC -!- TISSUE SPECIFICITY: Detected in placenta (at protein level).
CC Widely expressed. High expression is found in placenta followed by
CC lung and heart. Low expression in skeletal muscle, kidney and
CC pancreas.
CC -!- DOMAIN: Members of this family may change from a globular, soluble
CC state to a state where the N-terminal domain is inserted into the
CC membrane and functions as chloride channel. A conformation change
CC of the N-terminal domain is thought to expose hydrophobic surfaces
CC that trigger membrane insertion (By similarity).
CC -!- SIMILARITY: Belongs to the chloride channel CLIC family.
CC -!- SIMILARITY: Contains 1 GST C-terminal domain.
CC -!- SIMILARITY: Contains 1 GST N-terminal domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD16450.1; Type=Frameshift; Positions=28;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AL807752; CAI12764.1; -; Genomic_DNA.
DR EMBL; CH471090; EAW88329.1; -; Genomic_DNA.
DR EMBL; BC007012; AAH07012.2; -; mRNA.
DR EMBL; AF102166; AAD16450.1; ALT_FRAME; mRNA.
DR RefSeq; NP_004660.2; NM_004669.2.
DR UniGene; Hs.64746; -.
DR PDB; 3FY7; X-ray; 1.95 A; A/B=1-230.
DR PDB; 3KJY; X-ray; 1.95 A; A/B=1-230.
DR PDBsum; 3FY7; -.
DR PDBsum; 3KJY; -.
DR ProteinModelPortal; O95833; -.
DR SMR; O95833; 3-230.
DR STRING; 9606.ENSP00000360678; -.
DR TCDB; 1.A.12.1.7; the intracellular chloride channel (clic) family.
DR PhosphoSite; O95833; -.
DR PaxDb; O95833; -.
DR PeptideAtlas; O95833; -.
DR PRIDE; O95833; -.
DR DNASU; 9022; -.
DR Ensembl; ENST00000494426; ENSP00000419378; ENSG00000169583.
DR GeneID; 9022; -.
DR KEGG; hsa:9022; -.
DR UCSC; uc004ckj.1; human.
DR CTD; 9022; -.
DR GeneCards; GC09M139889; -.
DR HGNC; HGNC:2064; CLIC3.
DR HPA; HPA005963; -.
DR MIM; 606533; gene.
DR neXtProt; NX_O95833; -.
DR PharmGKB; PA26590; -.
DR eggNOG; NOG325572; -.
DR HOGENOM; HOG000231548; -.
DR HOVERGEN; HBG050994; -.
DR InParanoid; O95833; -.
DR KO; K05023; -.
DR OMA; PSCQRLF; -.
DR OrthoDB; EOG7X3QR3; -.
DR PhylomeDB; O95833; -.
DR EvolutionaryTrace; O95833; -.
DR GeneWiki; CLIC3; -.
DR GenomeRNAi; 9022; -.
DR NextBio; 33801; -.
DR PRO; PR:O95833; -.
DR ArrayExpress; O95833; -.
DR Bgee; O95833; -.
DR CleanEx; HS_CLIC3; -.
DR Genevestigator; O95833; -.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005254; F:chloride channel activity; IMP:UniProtKB.
DR GO; GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR Gene3D; 1.20.1050.10; -; 1.
DR Gene3D; 3.40.30.10; -; 1.
DR InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR InterPro; IPR004045; Glutathione_S-Trfase_N.
DR InterPro; IPR002946; Int_Cl_channel.
DR InterPro; IPR012336; Thioredoxin-like_fold.
DR PANTHER; PTHR11260:SF1; PTHR11260:SF1; 1.
DR Pfam; PF13417; GST_N_3; 1.
DR PRINTS; PR01263; INTCLCHANNEL.
DR SUPFAM; SSF47616; SSF47616; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS50405; GST_CTER; 1.
DR PROSITE; PS50404; GST_NTER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloride; Chloride channel; Complete proteome;
KW Cytoplasm; Disulfide bond; Ion channel; Ion transport; Membrane;
KW Nucleus; Polymorphism; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1 236 Chloride intracellular channel protein 3.
FT /FTId=PRO_0000144207.
FT TRANSMEM 24 44 Helical; (Potential).
FT DOMAIN 12 90 GST N-terminal.
FT DOMAIN 91 235 GST C-terminal.
FT REGION 1 88 Required for insertion into the membrane
FT (By similarity).
FT DISULFID 22 25 In soluble form.
FT VARIANT 38 38 P -> H (in dbSNP:rs2292923).
FT /FTId=VAR_020424.
FT STRAND 6 12
FT STRAND 16 19
FT HELIX 23 35
FT STRAND 40 44
FT STRAND 64 67
FT STRAND 70 72
FT HELIX 75 85
FT TURN 88 90
FT HELIX 99 103
FT TURN 104 107
FT HELIX 108 117
FT HELIX 121 123
FT HELIX 124 143
FT HELIX 147 152
FT STRAND 162 168
FT HELIX 171 191
FT HELIX 200 210
FT HELIX 213 216
FT HELIX 222 229
SQ SEQUENCE 236 AA; 26648 MW; CA20E66195950886 CRC64;
MAETKLQLFV KASEDGESVG HCPSCQRLFM VLLLKGVPFT LTTVDTRRSP DVLKDFAPGS
QLPILLYDSD AKTDTLQIED FLEETLGPPD FPSLAPRYRE SNTAGNDVFH KFSAFIKNPV
PAQDEALYQQ LLRALARLDS YLRAPLEHEL AGEPQLRESR RRFLDGDRLT LADCSLLPKL
HIVDTVCAHF RQAPIPAELR GVRRYLDSAM QEKEFKYTCP HSAEILAAYR PAVHPR
//
MIM
606533
*RECORD*
*FIELD* NO
606533
*FIELD* TI
*606533 CHLORIDE INTRACELLULAR CHANNEL 3; CLIC3
*FIELD* TX
Most chloride channels are localized to the plasma membrane. Members of
read morethe chloride intracellular channel (CLIC) family are expressed in the
nucleus as well as the plasma membrane, but they lack the
membrane-spanning domains characteristic of channel proteins.
CLONING
Using a yeast 2-hybrid screen of a fetal brain cDNA library with the C
terminus of rat Erk7 as bait, followed by EST database searching, Qian
et al. (1999) isolated a cDNA encoding CLIC3. Sequence analysis
predicted that the 208-amino acid protein is approximately 49% and 61%
identical to CLIC1 (602872) and CLIC2 (300138), respectively. CLIC3
contains a conserved transmembrane domain, N-terminal and central
phosphorylation sites, and an N-terminal N-myristoylation site.
Immunoprecipitation analysis confirmed the interaction of Erk7 and CLIC3
in transfected cells. Northern blot analysis revealed wide expression of
a 1.15-kb transcript, with highest levels in placenta and abundant
levels in heart and lung. Immunocytochemical analysis demonstrated
nuclear colocalization of Erk7 and CLIC3, with some cytoplasmic
expression of CLIC3. Further functional analysis suggested that CLIC3
mediates chloride transport across the plasma membrane. Based on
sequence homology, Qian et al. (1999) proposed that CLIC3, like CLIC1,
is a nuclear chloride transporter.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the CLIC3
gene to chromosome 9 (TMAP stSG28803).
*FIELD* RF
1. Qian, Z.; Okuhara, D.; Abe, M. K.; Rosner, M. R.: Molecular cloning
and characterization of a mitogen-activated protein kinase-associated
intracellular chloride channel. J. Biol. Chem. 274: 1621-1627, 1999.
*FIELD* CD
Paul J. Converse: 12/6/2001
*FIELD* ED
mgross: 12/06/2001
*RECORD*
*FIELD* NO
606533
*FIELD* TI
*606533 CHLORIDE INTRACELLULAR CHANNEL 3; CLIC3
*FIELD* TX
Most chloride channels are localized to the plasma membrane. Members of
read morethe chloride intracellular channel (CLIC) family are expressed in the
nucleus as well as the plasma membrane, but they lack the
membrane-spanning domains characteristic of channel proteins.
CLONING
Using a yeast 2-hybrid screen of a fetal brain cDNA library with the C
terminus of rat Erk7 as bait, followed by EST database searching, Qian
et al. (1999) isolated a cDNA encoding CLIC3. Sequence analysis
predicted that the 208-amino acid protein is approximately 49% and 61%
identical to CLIC1 (602872) and CLIC2 (300138), respectively. CLIC3
contains a conserved transmembrane domain, N-terminal and central
phosphorylation sites, and an N-terminal N-myristoylation site.
Immunoprecipitation analysis confirmed the interaction of Erk7 and CLIC3
in transfected cells. Northern blot analysis revealed wide expression of
a 1.15-kb transcript, with highest levels in placenta and abundant
levels in heart and lung. Immunocytochemical analysis demonstrated
nuclear colocalization of Erk7 and CLIC3, with some cytoplasmic
expression of CLIC3. Further functional analysis suggested that CLIC3
mediates chloride transport across the plasma membrane. Based on
sequence homology, Qian et al. (1999) proposed that CLIC3, like CLIC1,
is a nuclear chloride transporter.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the CLIC3
gene to chromosome 9 (TMAP stSG28803).
*FIELD* RF
1. Qian, Z.; Okuhara, D.; Abe, M. K.; Rosner, M. R.: Molecular cloning
and characterization of a mitogen-activated protein kinase-associated
intracellular chloride channel. J. Biol. Chem. 274: 1621-1627, 1999.
*FIELD* CD
Paul J. Converse: 12/6/2001
*FIELD* ED
mgross: 12/06/2001