Full text data of CNPY2
CNPY2
(MSAP, TMEM4, ZSIG9)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Protein canopy homolog 2 (MIR-interacting saposin-like protein; Putative secreted protein Zsig9; Transmembrane protein 4; Flags: Precursor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Protein canopy homolog 2 (MIR-interacting saposin-like protein; Putative secreted protein Zsig9; Transmembrane protein 4; Flags: Precursor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q9Y2B0
ID CNPY2_HUMAN Reviewed; 182 AA.
AC Q9Y2B0; B2R7B9; Q9UHE9;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1999, sequence version 1.
DT 22-JAN-2014, entry version 103.
DE RecName: Full=Protein canopy homolog 2;
DE AltName: Full=MIR-interacting saposin-like protein;
DE AltName: Full=Putative secreted protein Zsig9;
DE AltName: Full=Transmembrane protein 4;
DE Flags: Precursor;
GN Name=CNPY2; Synonyms=MSAP, TMEM4, ZSIG9; ORFNames=UNQ1943/PRO4426;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Gastric adenocarcinoma;
RX PubMed=10072769; DOI=10.1016/S0378-1119(99)00004-9;
RA Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S.;
RT "Selection of cDNAs encoding putative type II membrane proteins on the
RT cell surface from a human full-length cDNA bank.";
RL Gene 228:161-167(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYLIP, AND
RP TISSUE SPECIFICITY.
RX PubMed=12826659; DOI=10.1074/jbc.M306271200;
RA Bornhauser B.C., Olsson P.-A., Lindholm D.;
RT "MSAP is a novel MIR-interacting protein that enhances neurite
RT outgrowth and increases myosin regulatory light chain.";
RL J. Biol. Chem. 278:35412-35420(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J.,
RA O'Hara P.;
RT "Homo sapiens putative secreted protein.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Eye, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Positive regulator of neurite outgrowth by stabilizing
CC myosin regulatory light chain (MRLC). It prevents MIR-mediated
CC MRLC ubiquitination and its subsequent proteasomal degradation.
CC -!- SUBUNIT: Interacts with MYLIP/MIR.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum (Potential).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y2B0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y2B0-2; Sequence=VSP_010884, VSP_010885;
CC -!- TISSUE SPECIFICITY: Expressed in different tissues. Highest levels
CC are detected in adult placenta, liver and pancreas.
CC -!- SIMILARITY: Belongs to the canopy family.
CC -!- SIMILARITY: Contains 1 saposin B-type domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB015631; BAA76498.1; -; mRNA.
DR EMBL; AY032624; AAK38148.1; -; mRNA.
DR EMBL; AF186113; AAF01431.1; -; mRNA.
DR EMBL; AY359102; AAQ89460.1; -; mRNA.
DR EMBL; AK312921; BAG35766.1; -; mRNA.
DR EMBL; CH471054; EAW96926.1; -; Genomic_DNA.
DR EMBL; BC001027; AAH01027.1; -; mRNA.
DR EMBL; BC065015; AAH65015.1; -; mRNA.
DR RefSeq; NP_001177920.1; NM_001190991.1.
DR RefSeq; NP_055070.1; NM_014255.5.
DR UniGene; Hs.8752; -.
DR ProteinModelPortal; Q9Y2B0; -.
DR DIP; DIP-50705N; -.
DR IntAct; Q9Y2B0; 1.
DR MINT; MINT-3084309; -.
DR STRING; 9606.ENSP00000273308; -.
DR PhosphoSite; Q9Y2B0; -.
DR DMDM; 50401205; -.
DR PaxDb; Q9Y2B0; -.
DR PeptideAtlas; Q9Y2B0; -.
DR PRIDE; Q9Y2B0; -.
DR DNASU; 10330; -.
DR Ensembl; ENST00000273308; ENSP00000273308; ENSG00000257727.
DR GeneID; 10330; -.
DR KEGG; hsa:10330; -.
DR UCSC; uc001sku.2; human.
DR CTD; 10330; -.
DR GeneCards; GC12M056704; -.
DR HGNC; HGNC:13529; CNPY2.
DR HPA; HPA038465; -.
DR HPA; HPA038466; -.
DR MIM; 605861; gene.
DR neXtProt; NX_Q9Y2B0; -.
DR PharmGKB; PA162382584; -.
DR eggNOG; NOG280279; -.
DR HOGENOM; HOG000233996; -.
DR HOVERGEN; HBG052514; -.
DR InParanoid; Q9Y2B0; -.
DR PhylomeDB; Q9Y2B0; -.
DR ChiTaRS; CNPY2; human.
DR GenomeRNAi; 10330; -.
DR NextBio; 39165; -.
DR PRO; PR:Q9Y2B0; -.
DR ArrayExpress; Q9Y2B0; -.
DR Bgee; Q9Y2B0; -.
DR CleanEx; HS_CNPY2; -.
DR Genevestigator; Q9Y2B0; -.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR GO; GO:0010629; P:negative regulation of gene expression; ISS:BHF-UCL.
DR GO; GO:0045716; P:positive regulation of low-density lipoprotein particle receptor biosynthetic process; ISS:BHF-UCL.
DR GO; GO:0010988; P:regulation of low-density lipoprotein particle clearance; ISS:BHF-UCL.
DR InterPro; IPR021852; DUF3456.
DR InterPro; IPR008139; SaposinB.
DR Pfam; PF11938; DUF3456; 1.
DR PROSITE; PS00014; ER_TARGET; 1.
DR PROSITE; PS50015; SAP_B; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Complete proteome; Disulfide bond;
KW Endoplasmic reticulum; Phosphoprotein; Reference proteome; Signal.
FT SIGNAL 1 20 Potential.
FT CHAIN 21 182 Protein canopy homolog 2.
FT /FTId=PRO_0000031666.
FT DOMAIN 24 175 Saposin B-type.
FT MOTIF 179 182 Prevents secretion from ER (Potential).
FT MOD_RES 115 115 Phosphoserine (By similarity).
FT DISULFID 28 171 By similarity.
FT DISULFID 31 164 By similarity.
FT DISULFID 86 137 By similarity.
FT VAR_SEQ 70 84 PYARSEAHLTELLEE -> TVTVPPNKVAHSGFG (in
FT isoform 2).
FT /FTId=VSP_010884.
FT VAR_SEQ 85 182 Missing (in isoform 2).
FT /FTId=VSP_010885.
SQ SEQUENCE 182 AA; 20652 MW; BE726D302490733F CRC64;
MKGWGWLALL LGALLGTAWA RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP
DGSQSVVEVP YARSEAHLTE LLEEICDRMK EYGEQIDPST HRKNYVRVVG RNGESSELDL
QGIRIDSDIS GTLKFACESI VEEYEDELIE FFSREADNVK DKLCSKRTDL CDHALHISHD
EL
//
ID CNPY2_HUMAN Reviewed; 182 AA.
AC Q9Y2B0; B2R7B9; Q9UHE9;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1999, sequence version 1.
DT 22-JAN-2014, entry version 103.
DE RecName: Full=Protein canopy homolog 2;
DE AltName: Full=MIR-interacting saposin-like protein;
DE AltName: Full=Putative secreted protein Zsig9;
DE AltName: Full=Transmembrane protein 4;
DE Flags: Precursor;
GN Name=CNPY2; Synonyms=MSAP, TMEM4, ZSIG9; ORFNames=UNQ1943/PRO4426;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Gastric adenocarcinoma;
RX PubMed=10072769; DOI=10.1016/S0378-1119(99)00004-9;
RA Yokoyama-Kobayashi M., Yamaguchi T., Sekine S., Kato S.;
RT "Selection of cDNAs encoding putative type II membrane proteins on the
RT cell surface from a human full-length cDNA bank.";
RL Gene 228:161-167(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH MYLIP, AND
RP TISSUE SPECIFICITY.
RX PubMed=12826659; DOI=10.1074/jbc.M306271200;
RA Bornhauser B.C., Olsson P.-A., Lindholm D.;
RT "MSAP is a novel MIR-interacting protein that enhances neurite
RT outgrowth and increases myosin regulatory light chain.";
RL J. Biol. Chem. 278:35412-35420(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J.,
RA O'Hara P.;
RT "Homo sapiens putative secreted protein.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Eye, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Positive regulator of neurite outgrowth by stabilizing
CC myosin regulatory light chain (MRLC). It prevents MIR-mediated
CC MRLC ubiquitination and its subsequent proteasomal degradation.
CC -!- SUBUNIT: Interacts with MYLIP/MIR.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum (Potential).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y2B0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y2B0-2; Sequence=VSP_010884, VSP_010885;
CC -!- TISSUE SPECIFICITY: Expressed in different tissues. Highest levels
CC are detected in adult placenta, liver and pancreas.
CC -!- SIMILARITY: Belongs to the canopy family.
CC -!- SIMILARITY: Contains 1 saposin B-type domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB015631; BAA76498.1; -; mRNA.
DR EMBL; AY032624; AAK38148.1; -; mRNA.
DR EMBL; AF186113; AAF01431.1; -; mRNA.
DR EMBL; AY359102; AAQ89460.1; -; mRNA.
DR EMBL; AK312921; BAG35766.1; -; mRNA.
DR EMBL; CH471054; EAW96926.1; -; Genomic_DNA.
DR EMBL; BC001027; AAH01027.1; -; mRNA.
DR EMBL; BC065015; AAH65015.1; -; mRNA.
DR RefSeq; NP_001177920.1; NM_001190991.1.
DR RefSeq; NP_055070.1; NM_014255.5.
DR UniGene; Hs.8752; -.
DR ProteinModelPortal; Q9Y2B0; -.
DR DIP; DIP-50705N; -.
DR IntAct; Q9Y2B0; 1.
DR MINT; MINT-3084309; -.
DR STRING; 9606.ENSP00000273308; -.
DR PhosphoSite; Q9Y2B0; -.
DR DMDM; 50401205; -.
DR PaxDb; Q9Y2B0; -.
DR PeptideAtlas; Q9Y2B0; -.
DR PRIDE; Q9Y2B0; -.
DR DNASU; 10330; -.
DR Ensembl; ENST00000273308; ENSP00000273308; ENSG00000257727.
DR GeneID; 10330; -.
DR KEGG; hsa:10330; -.
DR UCSC; uc001sku.2; human.
DR CTD; 10330; -.
DR GeneCards; GC12M056704; -.
DR HGNC; HGNC:13529; CNPY2.
DR HPA; HPA038465; -.
DR HPA; HPA038466; -.
DR MIM; 605861; gene.
DR neXtProt; NX_Q9Y2B0; -.
DR PharmGKB; PA162382584; -.
DR eggNOG; NOG280279; -.
DR HOGENOM; HOG000233996; -.
DR HOVERGEN; HBG052514; -.
DR InParanoid; Q9Y2B0; -.
DR PhylomeDB; Q9Y2B0; -.
DR ChiTaRS; CNPY2; human.
DR GenomeRNAi; 10330; -.
DR NextBio; 39165; -.
DR PRO; PR:Q9Y2B0; -.
DR ArrayExpress; Q9Y2B0; -.
DR Bgee; Q9Y2B0; -.
DR CleanEx; HS_CNPY2; -.
DR Genevestigator; Q9Y2B0; -.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR GO; GO:0010629; P:negative regulation of gene expression; ISS:BHF-UCL.
DR GO; GO:0045716; P:positive regulation of low-density lipoprotein particle receptor biosynthetic process; ISS:BHF-UCL.
DR GO; GO:0010988; P:regulation of low-density lipoprotein particle clearance; ISS:BHF-UCL.
DR InterPro; IPR021852; DUF3456.
DR InterPro; IPR008139; SaposinB.
DR Pfam; PF11938; DUF3456; 1.
DR PROSITE; PS00014; ER_TARGET; 1.
DR PROSITE; PS50015; SAP_B; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Complete proteome; Disulfide bond;
KW Endoplasmic reticulum; Phosphoprotein; Reference proteome; Signal.
FT SIGNAL 1 20 Potential.
FT CHAIN 21 182 Protein canopy homolog 2.
FT /FTId=PRO_0000031666.
FT DOMAIN 24 175 Saposin B-type.
FT MOTIF 179 182 Prevents secretion from ER (Potential).
FT MOD_RES 115 115 Phosphoserine (By similarity).
FT DISULFID 28 171 By similarity.
FT DISULFID 31 164 By similarity.
FT DISULFID 86 137 By similarity.
FT VAR_SEQ 70 84 PYARSEAHLTELLEE -> TVTVPPNKVAHSGFG (in
FT isoform 2).
FT /FTId=VSP_010884.
FT VAR_SEQ 85 182 Missing (in isoform 2).
FT /FTId=VSP_010885.
SQ SEQUENCE 182 AA; 20652 MW; BE726D302490733F CRC64;
MKGWGWLALL LGALLGTAWA RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP
DGSQSVVEVP YARSEAHLTE LLEEICDRMK EYGEQIDPST HRKNYVRVVG RNGESSELDL
QGIRIDSDIS GTLKFACESI VEEYEDELIE FFSREADNVK DKLCSKRTDL CDHALHISHD
EL
//
MIM
605861
*RECORD*
*FIELD* NO
605861
*FIELD* TI
*605861 CANOPY 2, ZEBRAFISH, HOMOLOG OF; CNPY2
;;TRANSMEMBRANE PROTEIN 4; TMEM4;;
MIR-INTERACTING SAPOSIN-LIKE PROTEIN; MSAP
read more*FIELD* TX
CLONING
Instead of an N-terminal cleavable hydrophobic signal sequence (SS),
which is found in secreted or type I membrane proteins, type II membrane
proteins retain an uncleaved SS, called a signal anchor (SA), in the
membrane. With a type I SA, the N terminus is located in the cytosol,
whereas with a type II SA the N terminus is located outside the
membrane. Yokoyama-Kobayashi et al. (1995) developed an SS detection
system, called the fibrin sheet method, based on the detection of
secreted protein fused to a urokinase plasminogen activator protein
having fibrinolytic activity. Yokoyama-Kobayashi et al. (1999) adopted
this system, together with cell-fractionation analysis, to test for
fibrinolytic activity on the surface of cells transfected with fused
genes. They isolated a cDNA encoding TMEM4, which they termed HP10390,
from a gastric adenocarcinoma cDNA library. TMEM4 encodes a predicted
182-amino acid type II transmembrane protein.
Using MIR (MYLIP; 610082) as bait in a yeast 2-hybrid screen of a HeLa
cell cDNA library, followed by screening a fetal brain cDNA library,
Bornhauser et al. (2003) cloned TMEM4, which they designated MSAP. The
first 3 to 20 amino acids of MSAP constitute the SA region, which is
followed by a saposin (see PSAP; 176801)-like domain interrupted by 2
stretches of 30 and 40 amino acids. Northern blot analysis detected MSAP
expression in all adult and fetal tissues examined, with highest levels
in adult placenta, liver, and pancreas. Western blot analysis of
placenta and brain detected MSAP at an apparent molecular mass of 21 kD.
Immunofluorescence microscopy showed colocalization of Msap with Mir in
COS-7 cells. In cultured rodent primary hippocampal neurons, the 2
proteins localized around the nucleus and extended into the neurites.
GENE FUNCTION
Bornhauser et al. (2003) found that MIR and MSAP interacted, but only if
both proteins were full length. In rodent neuronal precursor cell lines,
MSAP overexpression increased the number of cells with neurites, and
downregulation of MSAP by RNA silencing inhibited neurite outgrowth.
MSAP stimulated neurite outgrowth in the presence or absence of NGF
(NGFB; 162030). Coexpression of MIR with MSAP reduced the stimulatory
effect of MSAP on neurite outgrowth. MIR overexpression also resulted in
ubiquitination of myosin regulatory light chain B (MRLC2; 609211), and
this ubiquitination could be counteracted by MSAP overexpression.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the CNPY2
gene to chromosome 12 (TMAP R22439).
*FIELD* RF
1. Bornhauser, B. C.; Olsson, P.-A.; Lindholm, D.: MSAP is a novel
MIR-interacting protein that enhances neurite outgrowth and increases
myosin regulatory light chain. J. Biol. Chem. 278: 35412-35420,
2003.
2. Yokoyama-Kobayashi, M.; Sugano, S.; Kato, T.; Kato, S.: A signal
sequence detection system using secreted protease activity as an indicator. Gene 163:
193-196, 1995.
3. Yokoyama-Kobayashi, M.; Yamaguchi, T.; Sekine, S.; Kato, S.: Selection
of cDNAs encoding putative type II membrane proteins on the cell surface
from a human full-length cDNA bank. Gene 228: 161-167, 1999.
*FIELD* CN
Patricia A. Hartz - updated: 4/21/2006
*FIELD* CD
Paul J. Converse: 4/20/2001
*FIELD* ED
alopez: 04/02/2009
mgross: 4/25/2006
terry: 4/21/2006
mgross: 4/20/2001
*RECORD*
*FIELD* NO
605861
*FIELD* TI
*605861 CANOPY 2, ZEBRAFISH, HOMOLOG OF; CNPY2
;;TRANSMEMBRANE PROTEIN 4; TMEM4;;
MIR-INTERACTING SAPOSIN-LIKE PROTEIN; MSAP
read more*FIELD* TX
CLONING
Instead of an N-terminal cleavable hydrophobic signal sequence (SS),
which is found in secreted or type I membrane proteins, type II membrane
proteins retain an uncleaved SS, called a signal anchor (SA), in the
membrane. With a type I SA, the N terminus is located in the cytosol,
whereas with a type II SA the N terminus is located outside the
membrane. Yokoyama-Kobayashi et al. (1995) developed an SS detection
system, called the fibrin sheet method, based on the detection of
secreted protein fused to a urokinase plasminogen activator protein
having fibrinolytic activity. Yokoyama-Kobayashi et al. (1999) adopted
this system, together with cell-fractionation analysis, to test for
fibrinolytic activity on the surface of cells transfected with fused
genes. They isolated a cDNA encoding TMEM4, which they termed HP10390,
from a gastric adenocarcinoma cDNA library. TMEM4 encodes a predicted
182-amino acid type II transmembrane protein.
Using MIR (MYLIP; 610082) as bait in a yeast 2-hybrid screen of a HeLa
cell cDNA library, followed by screening a fetal brain cDNA library,
Bornhauser et al. (2003) cloned TMEM4, which they designated MSAP. The
first 3 to 20 amino acids of MSAP constitute the SA region, which is
followed by a saposin (see PSAP; 176801)-like domain interrupted by 2
stretches of 30 and 40 amino acids. Northern blot analysis detected MSAP
expression in all adult and fetal tissues examined, with highest levels
in adult placenta, liver, and pancreas. Western blot analysis of
placenta and brain detected MSAP at an apparent molecular mass of 21 kD.
Immunofluorescence microscopy showed colocalization of Msap with Mir in
COS-7 cells. In cultured rodent primary hippocampal neurons, the 2
proteins localized around the nucleus and extended into the neurites.
GENE FUNCTION
Bornhauser et al. (2003) found that MIR and MSAP interacted, but only if
both proteins were full length. In rodent neuronal precursor cell lines,
MSAP overexpression increased the number of cells with neurites, and
downregulation of MSAP by RNA silencing inhibited neurite outgrowth.
MSAP stimulated neurite outgrowth in the presence or absence of NGF
(NGFB; 162030). Coexpression of MIR with MSAP reduced the stimulatory
effect of MSAP on neurite outgrowth. MIR overexpression also resulted in
ubiquitination of myosin regulatory light chain B (MRLC2; 609211), and
this ubiquitination could be counteracted by MSAP overexpression.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the CNPY2
gene to chromosome 12 (TMAP R22439).
*FIELD* RF
1. Bornhauser, B. C.; Olsson, P.-A.; Lindholm, D.: MSAP is a novel
MIR-interacting protein that enhances neurite outgrowth and increases
myosin regulatory light chain. J. Biol. Chem. 278: 35412-35420,
2003.
2. Yokoyama-Kobayashi, M.; Sugano, S.; Kato, T.; Kato, S.: A signal
sequence detection system using secreted protease activity as an indicator. Gene 163:
193-196, 1995.
3. Yokoyama-Kobayashi, M.; Yamaguchi, T.; Sekine, S.; Kato, S.: Selection
of cDNAs encoding putative type II membrane proteins on the cell surface
from a human full-length cDNA bank. Gene 228: 161-167, 1999.
*FIELD* CN
Patricia A. Hartz - updated: 4/21/2006
*FIELD* CD
Paul J. Converse: 4/20/2001
*FIELD* ED
alopez: 04/02/2009
mgross: 4/25/2006
terry: 4/21/2006
mgross: 4/20/2001