Full text data of DCTN6
DCTN6
(WS3)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Dynactin subunit 6 (Dynactin subunit p27; Protein WS-3)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Dynactin subunit 6 (Dynactin subunit p27; Protein WS-3)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
O00399
ID DCTN6_HUMAN Reviewed; 190 AA.
AC O00399; B2RAC1;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUL-1997, sequence version 1.
DT 22-JAN-2014, entry version 111.
DE RecName: Full=Dynactin subunit 6;
DE AltName: Full=Dynactin subunit p27;
DE AltName: Full=Protein WS-3;
GN Name=DCTN6; Synonyms=WS3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skin;
RX PubMed=9168138; DOI=10.1016/S0378-1119(96)00863-3;
RA Ichikawa K., Yamabe Y., Imamura O., Kuromitsu J., Sugawara K.,
RA Suzuki N., Shimamoto A., Matsumoto T., Tokutake Y., Kitao S.,
RA Kataoka H., Satoh M., Sugimoto M., Goto M., Sugawara M., Furuichi Y.;
RT "Cloning and characterization of a novel gene, WS-3, in human
RT chromosome 8p11-p12.";
RL Gene 189:277-287(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Substantia nigra;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), SUBUNIT, SUBCELLULAR LOCATION,
RP PHOSPHORYLATION AT THR-186, AND INTERACTION WITH PLK1.
RX PubMed=23455152; DOI=10.1038/emboj.2013.30;
RA Yeh T.Y., Kowalska A.K., Scipioni B.R., Cheong F.K., Zheng M.,
RA Derewenda U., Derewenda Z.S., Schroer T.A.;
RT "Dynactin helps target Polo-like kinase 1 to kinetochores via its
RT left-handed beta-helical p27 subunit.";
RL EMBO J. 32:1023-1035(2013).
CC -!- SUBUNIT: Member of the pointed-end complex of the dynactin
CC shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits
CC and ACTR10. Within the complex DCTN6 forms a heterodimer with
CC DCTN5. Interacts with PLK1.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (By similarity).
CC Chromosome, centromere, kinetochore.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- PTM: Phosphorylation at Thr-186 by CDK1 during mitotic
CC prometaphase creates a binding site for PLK1 that facilitates its
CC recruitment to kinetochores.
CC -!- SIMILARITY: Belongs to the dynactin subunits 5/6 family. Dynactin
CC subunit 6 subfamily.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; D84145; BAA19984.1; -; mRNA.
DR EMBL; CR456851; CAG33132.1; -; mRNA.
DR EMBL; AK314128; BAG36818.1; -; mRNA.
DR EMBL; CH471080; EAW63466.1; -; Genomic_DNA.
DR EMBL; BC013175; AAH13175.1; -; mRNA.
DR RefSeq; NP_006562.1; NM_006571.3.
DR UniGene; Hs.158427; -.
DR UniGene; Hs.721134; -.
DR PDB; 3TV0; X-ray; 2.15 A; A/B=1-190.
DR PDBsum; 3TV0; -.
DR ProteinModelPortal; O00399; -.
DR SMR; O00399; 9-157.
DR IntAct; O00399; 7.
DR STRING; 9606.ENSP00000221114; -.
DR PhosphoSite; O00399; -.
DR PaxDb; O00399; -.
DR PRIDE; O00399; -.
DR DNASU; 10671; -.
DR Ensembl; ENST00000221114; ENSP00000221114; ENSG00000104671.
DR GeneID; 10671; -.
DR KEGG; hsa:10671; -.
DR UCSC; uc003xhy.3; human.
DR CTD; 10671; -.
DR GeneCards; GC08P030013; -.
DR HGNC; HGNC:16964; DCTN6.
DR HPA; HPA024049; -.
DR HPA; HPA024558; -.
DR MIM; 612963; gene.
DR neXtProt; NX_O00399; -.
DR PharmGKB; PA134927975; -.
DR eggNOG; NOG149720; -.
DR HOGENOM; HOG000046876; -.
DR HOVERGEN; HBG051326; -.
DR InParanoid; O00399; -.
DR KO; K10428; -.
DR OMA; TIVHPKA; -.
DR OrthoDB; EOG7353Z2; -.
DR PhylomeDB; O00399; -.
DR Reactome; REACT_6900; Immune System.
DR GenomeRNAi; 10671; -.
DR NextBio; 40579; -.
DR PRO; PR:O00399; -.
DR ArrayExpress; O00399; -.
DR Bgee; O00399; -.
DR Genevestigator; O00399; -.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005869; C:dynactin complex; IEA:Ensembl.
DR GO; GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
DR InterPro; IPR027777; DCTN6.
DR InterPro; IPR001451; Hexapep_transf.
DR InterPro; IPR011004; Trimer_LpxA-like.
DR PANTHER; PTHR13072; PTHR13072; 1.
DR Pfam; PF00132; Hexapep; 2.
DR SUPFAM; SSF51161; SSF51161; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromosome; Complete proteome; Cytoplasm;
KW Cytoskeleton; Kinetochore; Phosphoprotein; Reference proteome.
FT CHAIN 1 190 Dynactin subunit 6.
FT /FTId=PRO_0000079830.
FT MOD_RES 186 186 Phosphothreonine; by CDK1.
FT STRAND 9 11
FT STRAND 21 29
FT STRAND 40 46
FT STRAND 48 50
FT STRAND 61 64
FT STRAND 82 84
FT STRAND 99 101
FT STRAND 117 119
FT STRAND 135 137
FT STRAND 141 145
FT STRAND 150 153
SQ SEQUENCE 190 AA; 20747 MW; 7D0B12C0029C77B7 CRC64;
MAEKTQKSVK IAPGAVVCVE SEIRGDVTIG PRTVIHPKAR IIAEAGPIVI GEGNLIEEQA
LIINAYPDNI TPDTEDPEPK PMIIGTNNVF EVGCYSQAMK MGDNNVIESK AYVGRNVILT
SGCIIGACCN LNTFEVIPEN TVIYGADCLR RVQTERPQPQ TLQLDFLMKI LPNYHHLKKT
MKGSSTPVKN
//
ID DCTN6_HUMAN Reviewed; 190 AA.
AC O00399; B2RAC1;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUL-1997, sequence version 1.
DT 22-JAN-2014, entry version 111.
DE RecName: Full=Dynactin subunit 6;
DE AltName: Full=Dynactin subunit p27;
DE AltName: Full=Protein WS-3;
GN Name=DCTN6; Synonyms=WS3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skin;
RX PubMed=9168138; DOI=10.1016/S0378-1119(96)00863-3;
RA Ichikawa K., Yamabe Y., Imamura O., Kuromitsu J., Sugawara K.,
RA Suzuki N., Shimamoto A., Matsumoto T., Tokutake Y., Kitao S.,
RA Kataoka H., Satoh M., Sugimoto M., Goto M., Sugawara M., Furuichi Y.;
RT "Cloning and characterization of a novel gene, WS-3, in human
RT chromosome 8p11-p12.";
RL Gene 189:277-287(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Substantia nigra;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), SUBUNIT, SUBCELLULAR LOCATION,
RP PHOSPHORYLATION AT THR-186, AND INTERACTION WITH PLK1.
RX PubMed=23455152; DOI=10.1038/emboj.2013.30;
RA Yeh T.Y., Kowalska A.K., Scipioni B.R., Cheong F.K., Zheng M.,
RA Derewenda U., Derewenda Z.S., Schroer T.A.;
RT "Dynactin helps target Polo-like kinase 1 to kinetochores via its
RT left-handed beta-helical p27 subunit.";
RL EMBO J. 32:1023-1035(2013).
CC -!- SUBUNIT: Member of the pointed-end complex of the dynactin
CC shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits
CC and ACTR10. Within the complex DCTN6 forms a heterodimer with
CC DCTN5. Interacts with PLK1.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (By similarity).
CC Chromosome, centromere, kinetochore.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- PTM: Phosphorylation at Thr-186 by CDK1 during mitotic
CC prometaphase creates a binding site for PLK1 that facilitates its
CC recruitment to kinetochores.
CC -!- SIMILARITY: Belongs to the dynactin subunits 5/6 family. Dynactin
CC subunit 6 subfamily.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; D84145; BAA19984.1; -; mRNA.
DR EMBL; CR456851; CAG33132.1; -; mRNA.
DR EMBL; AK314128; BAG36818.1; -; mRNA.
DR EMBL; CH471080; EAW63466.1; -; Genomic_DNA.
DR EMBL; BC013175; AAH13175.1; -; mRNA.
DR RefSeq; NP_006562.1; NM_006571.3.
DR UniGene; Hs.158427; -.
DR UniGene; Hs.721134; -.
DR PDB; 3TV0; X-ray; 2.15 A; A/B=1-190.
DR PDBsum; 3TV0; -.
DR ProteinModelPortal; O00399; -.
DR SMR; O00399; 9-157.
DR IntAct; O00399; 7.
DR STRING; 9606.ENSP00000221114; -.
DR PhosphoSite; O00399; -.
DR PaxDb; O00399; -.
DR PRIDE; O00399; -.
DR DNASU; 10671; -.
DR Ensembl; ENST00000221114; ENSP00000221114; ENSG00000104671.
DR GeneID; 10671; -.
DR KEGG; hsa:10671; -.
DR UCSC; uc003xhy.3; human.
DR CTD; 10671; -.
DR GeneCards; GC08P030013; -.
DR HGNC; HGNC:16964; DCTN6.
DR HPA; HPA024049; -.
DR HPA; HPA024558; -.
DR MIM; 612963; gene.
DR neXtProt; NX_O00399; -.
DR PharmGKB; PA134927975; -.
DR eggNOG; NOG149720; -.
DR HOGENOM; HOG000046876; -.
DR HOVERGEN; HBG051326; -.
DR InParanoid; O00399; -.
DR KO; K10428; -.
DR OMA; TIVHPKA; -.
DR OrthoDB; EOG7353Z2; -.
DR PhylomeDB; O00399; -.
DR Reactome; REACT_6900; Immune System.
DR GenomeRNAi; 10671; -.
DR NextBio; 40579; -.
DR PRO; PR:O00399; -.
DR ArrayExpress; O00399; -.
DR Bgee; O00399; -.
DR Genevestigator; O00399; -.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005869; C:dynactin complex; IEA:Ensembl.
DR GO; GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
DR InterPro; IPR027777; DCTN6.
DR InterPro; IPR001451; Hexapep_transf.
DR InterPro; IPR011004; Trimer_LpxA-like.
DR PANTHER; PTHR13072; PTHR13072; 1.
DR Pfam; PF00132; Hexapep; 2.
DR SUPFAM; SSF51161; SSF51161; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromosome; Complete proteome; Cytoplasm;
KW Cytoskeleton; Kinetochore; Phosphoprotein; Reference proteome.
FT CHAIN 1 190 Dynactin subunit 6.
FT /FTId=PRO_0000079830.
FT MOD_RES 186 186 Phosphothreonine; by CDK1.
FT STRAND 9 11
FT STRAND 21 29
FT STRAND 40 46
FT STRAND 48 50
FT STRAND 61 64
FT STRAND 82 84
FT STRAND 99 101
FT STRAND 117 119
FT STRAND 135 137
FT STRAND 141 145
FT STRAND 150 153
SQ SEQUENCE 190 AA; 20747 MW; 7D0B12C0029C77B7 CRC64;
MAEKTQKSVK IAPGAVVCVE SEIRGDVTIG PRTVIHPKAR IIAEAGPIVI GEGNLIEEQA
LIINAYPDNI TPDTEDPEPK PMIIGTNNVF EVGCYSQAMK MGDNNVIESK AYVGRNVILT
SGCIIGACCN LNTFEVIPEN TVIYGADCLR RVQTERPQPQ TLQLDFLMKI LPNYHHLKKT
MKGSSTPVKN
//
MIM
612963
*RECORD*
*FIELD* NO
612963
*FIELD* TI
*612963 DYNACTIN 6; DCTN6
;;WS3;;
p27
*FIELD* TX
DESCRIPTION
Dynactin (see 601143) is a multimeric protein essential for
read moreminus-end-directed transport driven by the microtubule-based motor
dynein (see DYNC1H1; 600112). DCTN6 is a subunit of the pointed-end
subcomplex of dynactin that is thought to interact with membranous cargo
(Parisi et al., 2004).
CLONING
By searching for genes in a region of chromosome 8 associated with
Werner syndrome (277700), followed by TAIL-PCR and 5-prime and 3-prime
RACE, Ichikawa et al. (1997) cloned DCTN6, which they called WS3. The
deduced 190-amino acid protein has a calculated molecular mass of 20.7
kD. It has several hydrophobic stretches, 4 potential phosphorylation
sites, 3 potential myristoylation sites, and an RGD motif that may
mediate cell attachment. Northern blot analysis detected a DCTN6
transcript of about 1.0 kb in all tissues examined. Zoo blot analysis
detected putative orthologs in other species, including yeast.
By searching databases for proteins containing an isoleucine-patch motif
predicted to adopt a left-handed parallel beta-helix fold, Parisi et al.
(2004) identified human DCTN6, which they called p27.
GENE STRUCTURE
Ichikawa et al. (1997) determined that the DCTN6 gene contains 7 exons.
MAPPING
Using FISH and mouse-human hybrid cell lines, Ichikawa et al. (1997)
mapped the DCTN6 gene to chromosome 8p12-p11, telomeric to the RBPMS
gene (601558). Southern blot analysis detected a single DCTN6 gene.
*FIELD* RF
1. Ichikawa, K.; Yamabe, Y.; Imamura, O.; Kuromitsu, J.; Sugawara,
K.; Suzuki, N.; Shimamoto, A.; Matsumoto, T.; Tokutake, Y.; Kitao,
S.; Kataoka, H.; Satoh, M.; Sugimoto, M.; Goto, M.; Sugawara, M.;
Furuichi, Y.: Cloning and characterization of a novel gene, WS-3,
in human chromosome 8p11-p12. Gene 189: 277-287, 1997.
2. Parisi, G.; Fornasari, M. S.; Echave, J.: Dynactins p25 and p27
are predicted to adopt the L-beta-H fold. FEBS Lett. 562: 1-4, 2004.
*FIELD* CD
Patricia A. Hartz: 8/13/2009
*FIELD* ED
mgross: 08/13/2009
*RECORD*
*FIELD* NO
612963
*FIELD* TI
*612963 DYNACTIN 6; DCTN6
;;WS3;;
p27
*FIELD* TX
DESCRIPTION
Dynactin (see 601143) is a multimeric protein essential for
read moreminus-end-directed transport driven by the microtubule-based motor
dynein (see DYNC1H1; 600112). DCTN6 is a subunit of the pointed-end
subcomplex of dynactin that is thought to interact with membranous cargo
(Parisi et al., 2004).
CLONING
By searching for genes in a region of chromosome 8 associated with
Werner syndrome (277700), followed by TAIL-PCR and 5-prime and 3-prime
RACE, Ichikawa et al. (1997) cloned DCTN6, which they called WS3. The
deduced 190-amino acid protein has a calculated molecular mass of 20.7
kD. It has several hydrophobic stretches, 4 potential phosphorylation
sites, 3 potential myristoylation sites, and an RGD motif that may
mediate cell attachment. Northern blot analysis detected a DCTN6
transcript of about 1.0 kb in all tissues examined. Zoo blot analysis
detected putative orthologs in other species, including yeast.
By searching databases for proteins containing an isoleucine-patch motif
predicted to adopt a left-handed parallel beta-helix fold, Parisi et al.
(2004) identified human DCTN6, which they called p27.
GENE STRUCTURE
Ichikawa et al. (1997) determined that the DCTN6 gene contains 7 exons.
MAPPING
Using FISH and mouse-human hybrid cell lines, Ichikawa et al. (1997)
mapped the DCTN6 gene to chromosome 8p12-p11, telomeric to the RBPMS
gene (601558). Southern blot analysis detected a single DCTN6 gene.
*FIELD* RF
1. Ichikawa, K.; Yamabe, Y.; Imamura, O.; Kuromitsu, J.; Sugawara,
K.; Suzuki, N.; Shimamoto, A.; Matsumoto, T.; Tokutake, Y.; Kitao,
S.; Kataoka, H.; Satoh, M.; Sugimoto, M.; Goto, M.; Sugawara, M.;
Furuichi, Y.: Cloning and characterization of a novel gene, WS-3,
in human chromosome 8p11-p12. Gene 189: 277-287, 1997.
2. Parisi, G.; Fornasari, M. S.; Echave, J.: Dynactins p25 and p27
are predicted to adopt the L-beta-H fold. FEBS Lett. 562: 1-4, 2004.
*FIELD* CD
Patricia A. Hartz: 8/13/2009
*FIELD* ED
mgross: 08/13/2009