Full text data of KATNAL1
KATNAL1
[Confidence: low (only semi-automatic identification from reviews)]
Katanin p60 ATPase-containing subunit A-like 1; Katanin p60 subunit A-like 1; 3.6.4.3 (p60 katanin-like 1)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Katanin p60 ATPase-containing subunit A-like 1; Katanin p60 subunit A-like 1; 3.6.4.3 (p60 katanin-like 1)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q9BW62
ID KATL1_HUMAN Reviewed; 490 AA.
AC Q9BW62; A8K5X4;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-2001, sequence version 1.
DT 22-JAN-2014, entry version 90.
DE RecName: Full=Katanin p60 ATPase-containing subunit A-like 1;
DE Short=Katanin p60 subunit A-like 1;
DE EC=3.6.4.3;
DE AltName: Full=p60 katanin-like 1;
GN Name=KATNAL1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E.,
RA Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.,
RA Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R.,
RA Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S.,
RA Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M.,
RA Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J.,
RA Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E.,
RA Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L.,
RA Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J.,
RA Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S.,
RA Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J.,
RA Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M.,
RA King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A.,
RA Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S.,
RA Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I.,
RA Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S.,
RA Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A.,
RA Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B.,
RA Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L.,
RA Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M.,
RA Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22654668; DOI=10.1371/journal.pgen.1002697;
RA Smith L.B., Milne L., Nelson N., Eddie S., Brown P., Atanassova N.,
RA O'Bryan M.K., O'Donnell L., Rhodes D., Wells S., Napper D., Nolan P.,
RA Lalanne Z., Cheeseman M., Peters J.;
RT "KATNAL1 regulation of Sertoli cell microtubule dynamics is essential
RT for spermiogenesis and male fertility.";
RL PLoS Genet. 8:E1002697-E1002697(2012).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Regulates microtubule dynamics in Sertoli cells, a
CC process that is essential for spermiogenesis and male fertility.
CC Severs microtubules in an ATP-dependent manner, promoting rapid
CC reorganization of cellular microtubule arrays (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC -!- TISSUE SPECIFICITY: Expressed in testis, restricted to Sertoli
CC cells (at protein level).
CC -!- SIMILARITY: Belongs to the AAA ATPase family. Katanin p60 subunit
CC A1 subfamily. A-like 1 sub-subfamily.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AK291439; BAF84128.1; -; mRNA.
DR EMBL; AL356750; CAI13718.1; -; Genomic_DNA.
DR EMBL; CH471075; EAX08452.1; -; Genomic_DNA.
DR EMBL; BC000612; AAH00612.1; -; mRNA.
DR RefSeq; NP_001014402.1; NM_001014380.2.
DR RefSeq; NP_115492.1; NM_032116.4.
DR RefSeq; XP_005266631.1; XM_005266574.1.
DR UniGene; Hs.243596; -.
DR ProteinModelPortal; Q9BW62; -.
DR SMR; Q9BW62; 1-72, 198-460.
DR IntAct; Q9BW62; 1.
DR MINT; MINT-1463957; -.
DR STRING; 9606.ENSP00000369989; -.
DR PhosphoSite; Q9BW62; -.
DR DMDM; 60390214; -.
DR PaxDb; Q9BW62; -.
DR PRIDE; Q9BW62; -.
DR DNASU; 84056; -.
DR Ensembl; ENST00000380615; ENSP00000369989; ENSG00000102781.
DR Ensembl; ENST00000380617; ENSP00000369991; ENSG00000102781.
DR GeneID; 84056; -.
DR KEGG; hsa:84056; -.
DR UCSC; uc001uss.4; human.
DR CTD; 84056; -.
DR GeneCards; GC13M030776; -.
DR HGNC; HGNC:28361; KATNAL1.
DR HPA; HPA046205; -.
DR MIM; 614764; gene.
DR neXtProt; NX_Q9BW62; -.
DR PharmGKB; PA134951885; -.
DR eggNOG; COG0464; -.
DR HOGENOM; HOG000225142; -.
DR HOVERGEN; HBG057074; -.
DR InParanoid; Q9BW62; -.
DR KO; K07767; -.
DR OMA; GVMQQIQ; -.
DR OrthoDB; EOG7SFHWZ; -.
DR GenomeRNAi; 84056; -.
DR NextBio; 73193; -.
DR PRO; PR:Q9BW62; -.
DR ArrayExpress; Q9BW62; -.
DR Bgee; Q9BW62; -.
DR CleanEx; HS_KATNAL1; -.
DR Genevestigator; Q9BW62; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008568; F:microtubule-severing ATPase activity; IEA:UniProtKB-EC.
DR GO; GO:0051013; P:microtubule severing; IEA:Ensembl.
DR GO; GO:0007283; P:spermatogenesis; IEA:Ensembl.
DR HAMAP; MF_03023; Katanin_p60_A1; 1; -.
DR HAMAP; MF_03024; Katanin_p60_AL1; 1; -.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR028596; KATNA1.
DR InterPro; IPR028594; Katnal1_chordates.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015415; Vps4_C.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF09336; Vps4_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton;
KW Hydrolase; Microtubule; Nucleotide-binding; Reference proteome.
FT CHAIN 1 490 Katanin p60 ATPase-containing subunit A-
FT like 1.
FT /FTId=PRO_0000084601.
FT NP_BIND 248 255 ATP (Potential).
FT MOD_RES 1 1 N-acetylmethionine.
SQ SEQUENCE 490 AA; 55392 MW; CB8E51A77E5CB487 CRC64;
MNLAEICDNA KKGREYALLG NYDSSMVYYQ GVMQQIQRHC QSVRDPAIKG KWQQVRQELL
EEYEQVKSIV STLESFKIDK PPDFPVSCQD EPFRDPAVWP PPVPAEHRAP PQIRRPNREV
RPLRKEMAGV GARGPVGRAH PISKSEKPST SRDKDYRARG RDDKGRKNMQ DGASDGEMPK
FDGAGYDKDL VEALERDIVS RNPSIHWDDI ADLEEAKKLL REAVVLPMWM PDFFKGIRRP
WKGVLMVGPP GTGKTMLAKA VATECGTTFF NVSSSTLTSK YRGESEKLVR LLFEMARFYA
PTTIFIDEID SICSRRGTSD EHEASRRVKS ELLIQMDGVG GALENDDPSK MVMVLAATNF
PWDIDEALRR RLEKRIYIPL PTAKGRAELL KINLREVELD PDIQLEDIAE KIEGYSGADI
TNVCRDASLM AMRRRINGLS PEEIRALSKE ELQMPVTKGD FELALKKIAK SVSAADLEKY
EKWMVEFGSA
//
ID KATL1_HUMAN Reviewed; 490 AA.
AC Q9BW62; A8K5X4;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-2001, sequence version 1.
DT 22-JAN-2014, entry version 90.
DE RecName: Full=Katanin p60 ATPase-containing subunit A-like 1;
DE Short=Katanin p60 subunit A-like 1;
DE EC=3.6.4.3;
DE AltName: Full=p60 katanin-like 1;
GN Name=KATNAL1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E.,
RA Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.,
RA Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R.,
RA Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S.,
RA Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M.,
RA Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J.,
RA Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E.,
RA Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L.,
RA Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J.,
RA Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S.,
RA Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J.,
RA Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M.,
RA King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A.,
RA Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S.,
RA Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I.,
RA Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S.,
RA Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A.,
RA Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B.,
RA Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L.,
RA Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M.,
RA Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22654668; DOI=10.1371/journal.pgen.1002697;
RA Smith L.B., Milne L., Nelson N., Eddie S., Brown P., Atanassova N.,
RA O'Bryan M.K., O'Donnell L., Rhodes D., Wells S., Napper D., Nolan P.,
RA Lalanne Z., Cheeseman M., Peters J.;
RT "KATNAL1 regulation of Sertoli cell microtubule dynamics is essential
RT for spermiogenesis and male fertility.";
RL PLoS Genet. 8:E1002697-E1002697(2012).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Regulates microtubule dynamics in Sertoli cells, a
CC process that is essential for spermiogenesis and male fertility.
CC Severs microtubules in an ATP-dependent manner, promoting rapid
CC reorganization of cellular microtubule arrays (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC -!- TISSUE SPECIFICITY: Expressed in testis, restricted to Sertoli
CC cells (at protein level).
CC -!- SIMILARITY: Belongs to the AAA ATPase family. Katanin p60 subunit
CC A1 subfamily. A-like 1 sub-subfamily.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AK291439; BAF84128.1; -; mRNA.
DR EMBL; AL356750; CAI13718.1; -; Genomic_DNA.
DR EMBL; CH471075; EAX08452.1; -; Genomic_DNA.
DR EMBL; BC000612; AAH00612.1; -; mRNA.
DR RefSeq; NP_001014402.1; NM_001014380.2.
DR RefSeq; NP_115492.1; NM_032116.4.
DR RefSeq; XP_005266631.1; XM_005266574.1.
DR UniGene; Hs.243596; -.
DR ProteinModelPortal; Q9BW62; -.
DR SMR; Q9BW62; 1-72, 198-460.
DR IntAct; Q9BW62; 1.
DR MINT; MINT-1463957; -.
DR STRING; 9606.ENSP00000369989; -.
DR PhosphoSite; Q9BW62; -.
DR DMDM; 60390214; -.
DR PaxDb; Q9BW62; -.
DR PRIDE; Q9BW62; -.
DR DNASU; 84056; -.
DR Ensembl; ENST00000380615; ENSP00000369989; ENSG00000102781.
DR Ensembl; ENST00000380617; ENSP00000369991; ENSG00000102781.
DR GeneID; 84056; -.
DR KEGG; hsa:84056; -.
DR UCSC; uc001uss.4; human.
DR CTD; 84056; -.
DR GeneCards; GC13M030776; -.
DR HGNC; HGNC:28361; KATNAL1.
DR HPA; HPA046205; -.
DR MIM; 614764; gene.
DR neXtProt; NX_Q9BW62; -.
DR PharmGKB; PA134951885; -.
DR eggNOG; COG0464; -.
DR HOGENOM; HOG000225142; -.
DR HOVERGEN; HBG057074; -.
DR InParanoid; Q9BW62; -.
DR KO; K07767; -.
DR OMA; GVMQQIQ; -.
DR OrthoDB; EOG7SFHWZ; -.
DR GenomeRNAi; 84056; -.
DR NextBio; 73193; -.
DR PRO; PR:Q9BW62; -.
DR ArrayExpress; Q9BW62; -.
DR Bgee; Q9BW62; -.
DR CleanEx; HS_KATNAL1; -.
DR Genevestigator; Q9BW62; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008568; F:microtubule-severing ATPase activity; IEA:UniProtKB-EC.
DR GO; GO:0051013; P:microtubule severing; IEA:Ensembl.
DR GO; GO:0007283; P:spermatogenesis; IEA:Ensembl.
DR HAMAP; MF_03023; Katanin_p60_A1; 1; -.
DR HAMAP; MF_03024; Katanin_p60_AL1; 1; -.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR028596; KATNA1.
DR InterPro; IPR028594; Katnal1_chordates.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015415; Vps4_C.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF09336; Vps4_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton;
KW Hydrolase; Microtubule; Nucleotide-binding; Reference proteome.
FT CHAIN 1 490 Katanin p60 ATPase-containing subunit A-
FT like 1.
FT /FTId=PRO_0000084601.
FT NP_BIND 248 255 ATP (Potential).
FT MOD_RES 1 1 N-acetylmethionine.
SQ SEQUENCE 490 AA; 55392 MW; CB8E51A77E5CB487 CRC64;
MNLAEICDNA KKGREYALLG NYDSSMVYYQ GVMQQIQRHC QSVRDPAIKG KWQQVRQELL
EEYEQVKSIV STLESFKIDK PPDFPVSCQD EPFRDPAVWP PPVPAEHRAP PQIRRPNREV
RPLRKEMAGV GARGPVGRAH PISKSEKPST SRDKDYRARG RDDKGRKNMQ DGASDGEMPK
FDGAGYDKDL VEALERDIVS RNPSIHWDDI ADLEEAKKLL REAVVLPMWM PDFFKGIRRP
WKGVLMVGPP GTGKTMLAKA VATECGTTFF NVSSSTLTSK YRGESEKLVR LLFEMARFYA
PTTIFIDEID SICSRRGTSD EHEASRRVKS ELLIQMDGVG GALENDDPSK MVMVLAATNF
PWDIDEALRR RLEKRIYIPL PTAKGRAELL KINLREVELD PDIQLEDIAE KIEGYSGADI
TNVCRDASLM AMRRRINGLS PEEIRALSKE ELQMPVTKGD FELALKKIAK SVSAADLEKY
EKWMVEFGSA
//
MIM
614764
*RECORD*
*FIELD* NO
614764
*FIELD* TI
*614764 KATANIN p60 SUBUNIT A-LIKE 1; KATNAL1
*FIELD* TX
DESCRIPTION
Katanin p60 subunit A-like-1 (KATNAL1) belongs to a family of
read moremicrotubule severing proteins (Smith et al., 2012).
CLONING
Smith et al. (2012) cloned the mouse Katnal1 gene. The deduced protein
contains an N-terminal microtubule interaction and trafficking (MIT)
domain, followed by an ATPase AAA-type core domain, and a C-terminal
Vps4_C domain that is important in oligomerization. RT-PCR detected 2
Katnal1 splice variants in all mouse tissues examined, with highest
expression in testis. Within embryonic mouse testis, Katnal1 expression
was detected from day 15.5 postcoitus onward, with expression restricted
to Sertoli cell cytoplasm. Immunohistochemical analysis revealed a
similar expression pattern in adult human testis.
GENE FUNCTION
Smith et al. (2012) found that overexpression of either Katnal1 or
Katanin p60 (KATNA1; 606696) in HEK293T cells resulted in disruption of
the microtubule lattice. Overexpression of Katnal1 in HT1080 cells
caused mitotic arrest and cell death.
MAPPING
Hartz (2012) mapped the KATNAL1 gene to chromosome 13q12.3 based on an
alignment of the KATNAL1 sequence (GenBank GENBANK BC000612) with the
genomic sequence (GRCh37).
Smith et al. (2012) mapped the mouse Katnal1 gene to distal chromosome
5.
ANIMAL MODEL
Smith et al. (2012) studied a line of mutant mice, generated by
N-ethyl-N-nitrosurea (ENU) mutagenesis, that resulted in infertility in
homozygous males only. Affected males also showed reduced testis mass,
but otherwise appeared normal. Smith et al. (2012) identified the
mutation as a homozygous T-to-G transition in exon 7 of the Katnal1
gene, resulting in a critical leu286-to-val substitution within the
conserved ATPase AAA-core domain of the protein. Histologically,
homozygous Katnal1 mutant testis showed disrupted microtubule network
within Sertoli cells, with premature exfoliation of spermatids from the
seminiferous epithelium. There was no cumulative degeneration with
aging. In wildtype testis, Katnal1 localized with Sertoli cell
microtubules throughout the basal and adluminal compartments, whereas in
mutant testis, Katnal1 localization was restricted to the basal
compartment of Sertoli cells. The Katnal1 mutation resulted in complete
loss of stabilized microtubules with C-terminal glutamic acid residues.
Smith et al. (2012) concluded that Katnal1 is a microtubule severing
protein involved in microtubule stabilization, and that loss of stable
microtubules in homozygous Katnal1 mutant mice permits premature release
of immature spermatids from testis.
*FIELD* RF
1. Hartz, P. A.: Personal Communication. Baltimore, Md. 7/31/2012.
2. Smith, L. B.; Milne, L.; Nelson, N.; Eddie, S.; Brown, P.; Atanassova,
N.; O'Bryan, M. K.; O'Donnell, L.; Rhodes, D.; Wells, S.; Napper,
D.; Nolan, P.; Lalanne, Z.; Cheeseman, M.; Peters, J.: KATNAL1 regulation
of Sertoli cell microtubule dynamics is essential for spermiogenesis
and male fertility. PLoS Genet. 8: e1002697, 2012. Note: Electronic
Article.
*FIELD* CD
Patricia A. Hartz: 8/15/2012
*FIELD* ED
terry: 08/20/2012
carol: 8/15/2012
*RECORD*
*FIELD* NO
614764
*FIELD* TI
*614764 KATANIN p60 SUBUNIT A-LIKE 1; KATNAL1
*FIELD* TX
DESCRIPTION
Katanin p60 subunit A-like-1 (KATNAL1) belongs to a family of
read moremicrotubule severing proteins (Smith et al., 2012).
CLONING
Smith et al. (2012) cloned the mouse Katnal1 gene. The deduced protein
contains an N-terminal microtubule interaction and trafficking (MIT)
domain, followed by an ATPase AAA-type core domain, and a C-terminal
Vps4_C domain that is important in oligomerization. RT-PCR detected 2
Katnal1 splice variants in all mouse tissues examined, with highest
expression in testis. Within embryonic mouse testis, Katnal1 expression
was detected from day 15.5 postcoitus onward, with expression restricted
to Sertoli cell cytoplasm. Immunohistochemical analysis revealed a
similar expression pattern in adult human testis.
GENE FUNCTION
Smith et al. (2012) found that overexpression of either Katnal1 or
Katanin p60 (KATNA1; 606696) in HEK293T cells resulted in disruption of
the microtubule lattice. Overexpression of Katnal1 in HT1080 cells
caused mitotic arrest and cell death.
MAPPING
Hartz (2012) mapped the KATNAL1 gene to chromosome 13q12.3 based on an
alignment of the KATNAL1 sequence (GenBank GENBANK BC000612) with the
genomic sequence (GRCh37).
Smith et al. (2012) mapped the mouse Katnal1 gene to distal chromosome
5.
ANIMAL MODEL
Smith et al. (2012) studied a line of mutant mice, generated by
N-ethyl-N-nitrosurea (ENU) mutagenesis, that resulted in infertility in
homozygous males only. Affected males also showed reduced testis mass,
but otherwise appeared normal. Smith et al. (2012) identified the
mutation as a homozygous T-to-G transition in exon 7 of the Katnal1
gene, resulting in a critical leu286-to-val substitution within the
conserved ATPase AAA-core domain of the protein. Histologically,
homozygous Katnal1 mutant testis showed disrupted microtubule network
within Sertoli cells, with premature exfoliation of spermatids from the
seminiferous epithelium. There was no cumulative degeneration with
aging. In wildtype testis, Katnal1 localized with Sertoli cell
microtubules throughout the basal and adluminal compartments, whereas in
mutant testis, Katnal1 localization was restricted to the basal
compartment of Sertoli cells. The Katnal1 mutation resulted in complete
loss of stabilized microtubules with C-terminal glutamic acid residues.
Smith et al. (2012) concluded that Katnal1 is a microtubule severing
protein involved in microtubule stabilization, and that loss of stable
microtubules in homozygous Katnal1 mutant mice permits premature release
of immature spermatids from testis.
*FIELD* RF
1. Hartz, P. A.: Personal Communication. Baltimore, Md. 7/31/2012.
2. Smith, L. B.; Milne, L.; Nelson, N.; Eddie, S.; Brown, P.; Atanassova,
N.; O'Bryan, M. K.; O'Donnell, L.; Rhodes, D.; Wells, S.; Napper,
D.; Nolan, P.; Lalanne, Z.; Cheeseman, M.; Peters, J.: KATNAL1 regulation
of Sertoli cell microtubule dynamics is essential for spermiogenesis
and male fertility. PLoS Genet. 8: e1002697, 2012. Note: Electronic
Article.
*FIELD* CD
Patricia A. Hartz: 8/15/2012
*FIELD* ED
terry: 08/20/2012
carol: 8/15/2012