Full text data of KPRP
KPRP
(C1orf45)
[Confidence: low (only semi-automatic identification from reviews)]
Keratinocyte proline-rich protein; hKPRP
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Keratinocyte proline-rich protein; hKPRP
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q5T749
ID KPRP_HUMAN Reviewed; 579 AA.
AC Q5T749;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 21-DEC-2004, sequence version 1.
DT 22-JAN-2014, entry version 71.
DE RecName: Full=Keratinocyte proline-rich protein;
DE Short=hKPRP;
GN Name=KPRP; Synonyms=C1orf45;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND INDUCTION.
RX PubMed=16297201; DOI=10.1111/j.0022-202X.2005.23887.x;
RA Lee W.-H., Jang S., Lee J.-S., Lee Y., Seo E.-Y., You K.-H.,
RA Lee S.-C., Nam K.I., Kim J.-M., Kee S.-H., Yang J.-M., Seo Y.-J.,
RA Park J.-K., Kim C.D., Lee J.-H.;
RT "Molecular cloning and expression of human keratinocyte proline-rich
RT protein (hKPRP), an epidermal marker isolated from calcium-induced
RT differentiating keratinocytes.";
RL J. Invest. Dermatol. 125:995-1000(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- TISSUE SPECIFICITY: Expressed in the upper layer of epidermis and
CC psoriasis (at protein level). Expressed in the upper layer of
CC epidermis and psoriasis.
CC -!- DEVELOPMENTAL STAGE: Expressed in the periderm of fetal skin from
CC 16th week gestational age and in the granular and horny layers of
CC the epidermis at 24th week gestational age and thereafter (at
CC protein level).
CC -!- INDUCTION: By calcium and in psoriatic lesions.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AY960854; AAX59055.1; -; mRNA.
DR EMBL; AL353779; CAI20978.1; -; Genomic_DNA.
DR RefSeq; NP_001020402.1; NM_001025231.1.
DR UniGene; Hs.149386; -.
DR ProteinModelPortal; Q5T749; -.
DR STRING; 9606.ENSP00000357762; -.
DR PhosphoSite; Q5T749; -.
DR DMDM; 74745319; -.
DR PaxDb; Q5T749; -.
DR PRIDE; Q5T749; -.
DR DNASU; 448834; -.
DR Ensembl; ENST00000368773; ENSP00000357762; ENSG00000203786.
DR Ensembl; ENST00000606109; ENSP00000475216; ENSG00000203786.
DR GeneID; 448834; -.
DR KEGG; hsa:448834; -.
DR UCSC; uc001fal.1; human.
DR CTD; 448834; -.
DR GeneCards; GC01P152730; -.
DR HGNC; HGNC:31823; KPRP.
DR HPA; HPA031988; -.
DR MIM; 613260; gene.
DR neXtProt; NX_Q5T749; -.
DR PharmGKB; PA162393640; -.
DR eggNOG; NOG123992; -.
DR HOGENOM; HOG000113243; -.
DR HOVERGEN; HBG081862; -.
DR InParanoid; Q5T749; -.
DR OMA; CDQQQIQ; -.
DR OrthoDB; EOG7TTQ8F; -.
DR GenomeRNAi; 448834; -.
DR NextBio; 111426; -.
DR PRO; PR:Q5T749; -.
DR Bgee; Q5T749; -.
DR CleanEx; HS_KPRP; -.
DR Genevestigator; Q5T749; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism;
KW Reference proteome.
FT CHAIN 1 579 Keratinocyte proline-rich protein.
FT /FTId=PRO_0000271008.
FT COMPBIAS 4 209 Gln-rich.
FT COMPBIAS 257 510 Pro-rich.
FT MOD_RES 394 394 Phosphoserine.
FT VARIANT 14 14 Q -> H (in dbSNP:rs17612167).
FT /FTId=VAR_029840.
FT VARIANT 37 37 V -> A (in dbSNP:rs944683).
FT /FTId=VAR_029841.
FT VARIANT 113 113 C -> R (in dbSNP:rs16834457).
FT /FTId=VAR_029842.
FT VARIANT 168 168 R -> H (in dbSNP:rs16834461).
FT /FTId=VAR_029843.
FT VARIANT 413 413 C -> S (in dbSNP:rs4329520).
FT /FTId=VAR_029844.
FT VARIANT 532 532 P -> T (in dbSNP:rs6703294).
FT /FTId=VAR_029845.
SQ SEQUENCE 579 AA; 64136 MW; AB29AE2276D15A04 CRC64;
MCDQQQIQCR LPLQQCCVKG PSFCSSQSPF AQSQVVVQAP CEMQIVDCPA SCPVQVCQVS
DQAPCQSQTT QVKCQSKTKQ VKGQAQCQSK TTQVKGQAAS QSQTSSVQSQ APCQSEVSYV
QCEASQPVQT CFVECAPVCY TETCYVECPV QNYVPCPAPQ PVQMYRGRPA VCQPQGRFST
QCQYQGSYSS CGPQFQSRAT CNNYTPQFQL RPSYSSCFPQ YRSRTSFSPC VPQCQTQGSY
GSFTEQHRSR STSRCLPPPR RLQLFPRSCS PPRRFEPCSS SYLPLRPSEG FPNYCTPPRR
SEPIYNSRCP RRPISSCSQR RGPKCRIEIS SPCCPRQVPP QRCPVEIPPI RRRSQSCGPQ
PSWGASCPEL RPHVEPRPLP SFCPPRRLDQ CPESPLQRCP PPAPRPRLRP EPCISLEPRP
RPLPRQLSEP CLYPEPLPAL RPTPRPVPLP RPGQCEIPEP RPCLQPCEHP EPCPRPEPIP
LPAPCPSPEP CRETWRSPSP CWGPNPVPYP GDLGCHESSP HRLDTEAPYC GPSSYNQGQE
SGAGCGPGDV FPERRGQDGH GDQGNAFAGV KGEAKSAYF
//
ID KPRP_HUMAN Reviewed; 579 AA.
AC Q5T749;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 21-DEC-2004, sequence version 1.
DT 22-JAN-2014, entry version 71.
DE RecName: Full=Keratinocyte proline-rich protein;
DE Short=hKPRP;
GN Name=KPRP; Synonyms=C1orf45;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND INDUCTION.
RX PubMed=16297201; DOI=10.1111/j.0022-202X.2005.23887.x;
RA Lee W.-H., Jang S., Lee J.-S., Lee Y., Seo E.-Y., You K.-H.,
RA Lee S.-C., Nam K.I., Kim J.-M., Kee S.-H., Yang J.-M., Seo Y.-J.,
RA Park J.-K., Kim C.D., Lee J.-H.;
RT "Molecular cloning and expression of human keratinocyte proline-rich
RT protein (hKPRP), an epidermal marker isolated from calcium-induced
RT differentiating keratinocytes.";
RL J. Invest. Dermatol. 125:995-1000(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- TISSUE SPECIFICITY: Expressed in the upper layer of epidermis and
CC psoriasis (at protein level). Expressed in the upper layer of
CC epidermis and psoriasis.
CC -!- DEVELOPMENTAL STAGE: Expressed in the periderm of fetal skin from
CC 16th week gestational age and in the granular and horny layers of
CC the epidermis at 24th week gestational age and thereafter (at
CC protein level).
CC -!- INDUCTION: By calcium and in psoriatic lesions.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AY960854; AAX59055.1; -; mRNA.
DR EMBL; AL353779; CAI20978.1; -; Genomic_DNA.
DR RefSeq; NP_001020402.1; NM_001025231.1.
DR UniGene; Hs.149386; -.
DR ProteinModelPortal; Q5T749; -.
DR STRING; 9606.ENSP00000357762; -.
DR PhosphoSite; Q5T749; -.
DR DMDM; 74745319; -.
DR PaxDb; Q5T749; -.
DR PRIDE; Q5T749; -.
DR DNASU; 448834; -.
DR Ensembl; ENST00000368773; ENSP00000357762; ENSG00000203786.
DR Ensembl; ENST00000606109; ENSP00000475216; ENSG00000203786.
DR GeneID; 448834; -.
DR KEGG; hsa:448834; -.
DR UCSC; uc001fal.1; human.
DR CTD; 448834; -.
DR GeneCards; GC01P152730; -.
DR HGNC; HGNC:31823; KPRP.
DR HPA; HPA031988; -.
DR MIM; 613260; gene.
DR neXtProt; NX_Q5T749; -.
DR PharmGKB; PA162393640; -.
DR eggNOG; NOG123992; -.
DR HOGENOM; HOG000113243; -.
DR HOVERGEN; HBG081862; -.
DR InParanoid; Q5T749; -.
DR OMA; CDQQQIQ; -.
DR OrthoDB; EOG7TTQ8F; -.
DR GenomeRNAi; 448834; -.
DR NextBio; 111426; -.
DR PRO; PR:Q5T749; -.
DR Bgee; Q5T749; -.
DR CleanEx; HS_KPRP; -.
DR Genevestigator; Q5T749; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism;
KW Reference proteome.
FT CHAIN 1 579 Keratinocyte proline-rich protein.
FT /FTId=PRO_0000271008.
FT COMPBIAS 4 209 Gln-rich.
FT COMPBIAS 257 510 Pro-rich.
FT MOD_RES 394 394 Phosphoserine.
FT VARIANT 14 14 Q -> H (in dbSNP:rs17612167).
FT /FTId=VAR_029840.
FT VARIANT 37 37 V -> A (in dbSNP:rs944683).
FT /FTId=VAR_029841.
FT VARIANT 113 113 C -> R (in dbSNP:rs16834457).
FT /FTId=VAR_029842.
FT VARIANT 168 168 R -> H (in dbSNP:rs16834461).
FT /FTId=VAR_029843.
FT VARIANT 413 413 C -> S (in dbSNP:rs4329520).
FT /FTId=VAR_029844.
FT VARIANT 532 532 P -> T (in dbSNP:rs6703294).
FT /FTId=VAR_029845.
SQ SEQUENCE 579 AA; 64136 MW; AB29AE2276D15A04 CRC64;
MCDQQQIQCR LPLQQCCVKG PSFCSSQSPF AQSQVVVQAP CEMQIVDCPA SCPVQVCQVS
DQAPCQSQTT QVKCQSKTKQ VKGQAQCQSK TTQVKGQAAS QSQTSSVQSQ APCQSEVSYV
QCEASQPVQT CFVECAPVCY TETCYVECPV QNYVPCPAPQ PVQMYRGRPA VCQPQGRFST
QCQYQGSYSS CGPQFQSRAT CNNYTPQFQL RPSYSSCFPQ YRSRTSFSPC VPQCQTQGSY
GSFTEQHRSR STSRCLPPPR RLQLFPRSCS PPRRFEPCSS SYLPLRPSEG FPNYCTPPRR
SEPIYNSRCP RRPISSCSQR RGPKCRIEIS SPCCPRQVPP QRCPVEIPPI RRRSQSCGPQ
PSWGASCPEL RPHVEPRPLP SFCPPRRLDQ CPESPLQRCP PPAPRPRLRP EPCISLEPRP
RPLPRQLSEP CLYPEPLPAL RPTPRPVPLP RPGQCEIPEP RPCLQPCEHP EPCPRPEPIP
LPAPCPSPEP CRETWRSPSP CWGPNPVPYP GDLGCHESSP HRLDTEAPYC GPSSYNQGQE
SGAGCGPGDV FPERRGQDGH GDQGNAFAGV KGEAKSAYF
//
MIM
613260
*RECORD*
*FIELD* NO
613260
*FIELD* TI
*613260 KERATINOCYTE PROLINE-RICH PROTEIN; KPRP
*FIELD* TX
CLONING
Using suppression subtractive hybridization to identify cDNA clones
read moreinduced by calcium in normal human epidermal keratinocytes, followed by
5-prime and 3-prime RACE, Lee et al. (2005) cloned KPRP. The deduced
579-amino acid protein has a calculated molecular mass of 64 kD. It has
a glutamine-rich N-terminal half, a proline-rich C-terminal half, and 8
putative N-myristoylation sites. Northern blot analysis of mouse tissues
detected Kprp expression in stomach and skin. In situ hybridization
revealed KPRP expression in the upper granular layer of differentiated
normal human skin. In human fetal skin, KPRP expression began in the
periderm at 16 weeks' estimated gestational age (EGA), and it was
expressed in the granular and horny layers of the epidermis at 24 weeks'
EGA and thereafter. In mice, expression began at embryonic day 17.5.
Western blot analysis detected KPRP at an apparent molecular mass of 70
kD.
GENE FUNCTION
Lee et al. (2005) found that elevated intracellular calcium resulted in
a time-dependent increase in KPRP expression in normal human epidermal
keratinocytes. Expression of KPRP was elevated in psoriatic lesions
compared with normal skin.
MAPPING
By genomic sequence analysis, Lee et al. (2005) mapped the KPRP gene to
the epidermal differentiation complex on chromosome 1q21.
*FIELD* RF
1. Lee, W.-H.; Jang, S.; Lee, J.-S.; Lee, Y.; Seo, E.-Y.; You, K.-H.;
Lee, S.-C.; Nam, K.-I.; Kim, J.-M.; Kee, S.-H.; Yang, J.-M.; Seo,
Y.-J.; Park, J.-K.; Kim, C. D.; Lee, J.-H.: Molecular cloning and
expression of human keratinocyte proline-rich protein (hKPRP), an
epidermal marker isolated from calcium-induced differentiating keratinocytes. J.
Invest. Derm. 125: 995-1000, 2005.
*FIELD* CD
Patricia A. Hartz: 2/19/2010
*FIELD* ED
mgross: 02/19/2010
*RECORD*
*FIELD* NO
613260
*FIELD* TI
*613260 KERATINOCYTE PROLINE-RICH PROTEIN; KPRP
*FIELD* TX
CLONING
Using suppression subtractive hybridization to identify cDNA clones
read moreinduced by calcium in normal human epidermal keratinocytes, followed by
5-prime and 3-prime RACE, Lee et al. (2005) cloned KPRP. The deduced
579-amino acid protein has a calculated molecular mass of 64 kD. It has
a glutamine-rich N-terminal half, a proline-rich C-terminal half, and 8
putative N-myristoylation sites. Northern blot analysis of mouse tissues
detected Kprp expression in stomach and skin. In situ hybridization
revealed KPRP expression in the upper granular layer of differentiated
normal human skin. In human fetal skin, KPRP expression began in the
periderm at 16 weeks' estimated gestational age (EGA), and it was
expressed in the granular and horny layers of the epidermis at 24 weeks'
EGA and thereafter. In mice, expression began at embryonic day 17.5.
Western blot analysis detected KPRP at an apparent molecular mass of 70
kD.
GENE FUNCTION
Lee et al. (2005) found that elevated intracellular calcium resulted in
a time-dependent increase in KPRP expression in normal human epidermal
keratinocytes. Expression of KPRP was elevated in psoriatic lesions
compared with normal skin.
MAPPING
By genomic sequence analysis, Lee et al. (2005) mapped the KPRP gene to
the epidermal differentiation complex on chromosome 1q21.
*FIELD* RF
1. Lee, W.-H.; Jang, S.; Lee, J.-S.; Lee, Y.; Seo, E.-Y.; You, K.-H.;
Lee, S.-C.; Nam, K.-I.; Kim, J.-M.; Kee, S.-H.; Yang, J.-M.; Seo,
Y.-J.; Park, J.-K.; Kim, C. D.; Lee, J.-H.: Molecular cloning and
expression of human keratinocyte proline-rich protein (hKPRP), an
epidermal marker isolated from calcium-induced differentiating keratinocytes. J.
Invest. Derm. 125: 995-1000, 2005.
*FIELD* CD
Patricia A. Hartz: 2/19/2010
*FIELD* ED
mgross: 02/19/2010