Full text data of HDDC3
HDDC3
(MESH1)
[Confidence: low (only semi-automatic identification from reviews)]
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1; 3.1.7.2 (HD domain-containing protein 3; Metazoan SpoT homolog 1; MESH1; Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1; 3.1.7.2 (HD domain-containing protein 3; Metazoan SpoT homolog 1; MESH1; Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q8N4P3
ID MESH1_HUMAN Reviewed; 179 AA.
AC Q8N4P3;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
read moreDT 09-JAN-2007, sequence version 3.
DT 22-JAN-2014, entry version 87.
DE RecName: Full=Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1;
DE EC=3.1.7.2;
DE AltName: Full=HD domain-containing protein 3;
DE AltName: Full=Metazoan SpoT homolog 1;
DE Short=MESH1;
DE AltName: Full=Penta-phosphate guanosine-3'-pyrophosphohydrolase;
DE Short=(ppGpp)ase;
GN Name=HDDC3; Synonyms=MESH1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R.,
RA Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G.,
RA Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A.,
RA Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W.,
RA Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X.,
RA Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K.,
RA Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S.,
RA Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human
RT chromosome 15.";
RL Nature 440:671-675(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Cervix;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-25; LYS-97 AND LYS-123, AND
RP MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 2-179, FUNCTION, CATALYTIC
RP ACTIVITY, ACTIVE SITE, COFACTOR, MUTAGENESIS OF ARG-24; GLU-65 AND
RP ASP-66, AND MANGANESE-BINDING SITES.
RX PubMed=20818390; DOI=10.1038/nsmb.1906;
RA Sun D., Lee G., Lee J.H., Kim H.-Y., Rhee H.W., Park S.-Y., Kim K.J.,
RA Kim Y., Kim B.Y., Hong J.-I., Park C., Choy H.E., Kim J.H., Jeon Y.H.,
RA Chung J.;
RT "A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in
RT starvation responses.";
RL Nat. Struct. Mol. Biol. 17:1188-1194(2010).
CC -!- FUNCTION: ppGpp hydrolyzing enzyme involved in starvation
CC response.
CC -!- CATALYTIC ACTIVITY: Guanosine 3',5'-bis(diphosphate) + H(2)O =
CC guanosine 5'-diphosphate + diphosphate.
CC -!- COFACTOR: Manganese.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N4P3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N4P3-2; Sequence=VSP_021866, VSP_021867;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the MESH1 family.
CC -!- SIMILARITY: Contains 1 HD domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AC068831; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC033794; AAH33794.1; -; mRNA.
DR RefSeq; NP_001273380.1; NM_001286451.1.
DR RefSeq; NP_940929.1; NM_198527.3.
DR UniGene; Hs.349979; -.
DR PDB; 3NR1; X-ray; 1.90 A; A/B=2-179.
DR PDBsum; 3NR1; -.
DR ProteinModelPortal; Q8N4P3; -.
DR SMR; Q8N4P3; 2-179.
DR IntAct; Q8N4P3; 1.
DR MINT; MINT-1475376; -.
DR STRING; 9606.ENSP00000330721; -.
DR PhosphoSite; Q8N4P3; -.
DR DMDM; 122065202; -.
DR PaxDb; Q8N4P3; -.
DR PRIDE; Q8N4P3; -.
DR DNASU; 374659; -.
DR Ensembl; ENST00000330334; ENSP00000330721; ENSG00000184508.
DR Ensembl; ENST00000394272; ENSP00000377814; ENSG00000184508.
DR GeneID; 374659; -.
DR KEGG; hsa:374659; -.
DR CTD; 374659; -.
DR GeneCards; GC15M091474; -.
DR H-InvDB; HIX0012581; -.
DR HGNC; HGNC:30522; HDDC3.
DR HPA; HPA040895; -.
DR neXtProt; NX_Q8N4P3; -.
DR PharmGKB; PA142671696; -.
DR eggNOG; NOG113345; -.
DR HOVERGEN; HBG081594; -.
DR InParanoid; Q8N4P3; -.
DR KO; K01139; -.
DR OMA; NRCTPEG; -.
DR OrthoDB; EOG7X3QTC; -.
DR EvolutionaryTrace; Q8N4P3; -.
DR GenomeRNAi; 374659; -.
DR NextBio; 100241; -.
DR PRO; PR:Q8N4P3; -.
DR ArrayExpress; Q8N4P3; -.
DR Bgee; Q8N4P3; -.
DR CleanEx; HS_HDDC3; -.
DR Genevestigator; Q8N4P3; -.
DR GO; GO:0008893; F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008152; P:metabolic process; IEA:GOC.
DR InterPro; IPR003607; HD/PDEase_dom.
DR SMART; SM00471; HDc; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Complete proteome;
KW Hydrolase; Manganese; Metal-binding; Reference proteome.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 179 Guanosine-3',5'-bis(diphosphate) 3'-
FT pyrophosphohydrolase MESH1.
FT /FTId=PRO_0000263110.
FT DOMAIN 32 127 HD.
FT ACT_SITE 65 65 Nucleophile (Probable).
FT ACT_SITE 66 66 Nucleophile (Probable).
FT METAL 35 35 Manganese.
FT METAL 61 61 Manganese.
FT METAL 62 62 Manganese.
FT METAL 122 122 Manganese.
FT MOD_RES 2 2 N-acetylglycine.
FT MOD_RES 25 25 N6-acetyllysine.
FT MOD_RES 97 97 N6-acetyllysine.
FT MOD_RES 123 123 N6-acetyllysine.
FT VAR_SEQ 137 140 GWSE -> VKIQ (in isoform 2).
FT /FTId=VSP_021866.
FT VAR_SEQ 141 179 Missing (in isoform 2).
FT /FTId=VSP_021867.
FT MUTAGEN 24 24 R->A: Abrogates ppGpp hydrolase activity.
FT MUTAGEN 65 65 E->A: Abrogates ppGpp hydrolase activity.
FT MUTAGEN 66 66 D->A: Reduces ppGpp hydrolase activity.
FT HELIX 4 18
FT TURN 19 21
FT HELIX 34 45
FT HELIX 52 60
FT HELIX 63 66
FT HELIX 71 88
FT HELIX 97 107
FT HELIX 108 110
FT HELIX 113 132
FT HELIX 140 157
FT HELIX 162 173
FT TURN 174 176
SQ SEQUENCE 179 AA; 20329 MW; E0946B0FA7BD8109 CRC64;
MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL
HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL
ADKLYNLRDL NRCTPEGWSE HRVQEYFEWA AQVVKGLQGT NRQLEEALKH LFKQRGLTI
//
ID MESH1_HUMAN Reviewed; 179 AA.
AC Q8N4P3;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
read moreDT 09-JAN-2007, sequence version 3.
DT 22-JAN-2014, entry version 87.
DE RecName: Full=Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1;
DE EC=3.1.7.2;
DE AltName: Full=HD domain-containing protein 3;
DE AltName: Full=Metazoan SpoT homolog 1;
DE Short=MESH1;
DE AltName: Full=Penta-phosphate guanosine-3'-pyrophosphohydrolase;
DE Short=(ppGpp)ase;
GN Name=HDDC3; Synonyms=MESH1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R.,
RA Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G.,
RA Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A.,
RA Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W.,
RA Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X.,
RA Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K.,
RA Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S.,
RA Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human
RT chromosome 15.";
RL Nature 440:671-675(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Cervix;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-25; LYS-97 AND LYS-123, AND
RP MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 2-179, FUNCTION, CATALYTIC
RP ACTIVITY, ACTIVE SITE, COFACTOR, MUTAGENESIS OF ARG-24; GLU-65 AND
RP ASP-66, AND MANGANESE-BINDING SITES.
RX PubMed=20818390; DOI=10.1038/nsmb.1906;
RA Sun D., Lee G., Lee J.H., Kim H.-Y., Rhee H.W., Park S.-Y., Kim K.J.,
RA Kim Y., Kim B.Y., Hong J.-I., Park C., Choy H.E., Kim J.H., Jeon Y.H.,
RA Chung J.;
RT "A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in
RT starvation responses.";
RL Nat. Struct. Mol. Biol. 17:1188-1194(2010).
CC -!- FUNCTION: ppGpp hydrolyzing enzyme involved in starvation
CC response.
CC -!- CATALYTIC ACTIVITY: Guanosine 3',5'-bis(diphosphate) + H(2)O =
CC guanosine 5'-diphosphate + diphosphate.
CC -!- COFACTOR: Manganese.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8N4P3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N4P3-2; Sequence=VSP_021866, VSP_021867;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the MESH1 family.
CC -!- SIMILARITY: Contains 1 HD domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AC068831; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC033794; AAH33794.1; -; mRNA.
DR RefSeq; NP_001273380.1; NM_001286451.1.
DR RefSeq; NP_940929.1; NM_198527.3.
DR UniGene; Hs.349979; -.
DR PDB; 3NR1; X-ray; 1.90 A; A/B=2-179.
DR PDBsum; 3NR1; -.
DR ProteinModelPortal; Q8N4P3; -.
DR SMR; Q8N4P3; 2-179.
DR IntAct; Q8N4P3; 1.
DR MINT; MINT-1475376; -.
DR STRING; 9606.ENSP00000330721; -.
DR PhosphoSite; Q8N4P3; -.
DR DMDM; 122065202; -.
DR PaxDb; Q8N4P3; -.
DR PRIDE; Q8N4P3; -.
DR DNASU; 374659; -.
DR Ensembl; ENST00000330334; ENSP00000330721; ENSG00000184508.
DR Ensembl; ENST00000394272; ENSP00000377814; ENSG00000184508.
DR GeneID; 374659; -.
DR KEGG; hsa:374659; -.
DR CTD; 374659; -.
DR GeneCards; GC15M091474; -.
DR H-InvDB; HIX0012581; -.
DR HGNC; HGNC:30522; HDDC3.
DR HPA; HPA040895; -.
DR neXtProt; NX_Q8N4P3; -.
DR PharmGKB; PA142671696; -.
DR eggNOG; NOG113345; -.
DR HOVERGEN; HBG081594; -.
DR InParanoid; Q8N4P3; -.
DR KO; K01139; -.
DR OMA; NRCTPEG; -.
DR OrthoDB; EOG7X3QTC; -.
DR EvolutionaryTrace; Q8N4P3; -.
DR GenomeRNAi; 374659; -.
DR NextBio; 100241; -.
DR PRO; PR:Q8N4P3; -.
DR ArrayExpress; Q8N4P3; -.
DR Bgee; Q8N4P3; -.
DR CleanEx; HS_HDDC3; -.
DR Genevestigator; Q8N4P3; -.
DR GO; GO:0008893; F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008152; P:metabolic process; IEA:GOC.
DR InterPro; IPR003607; HD/PDEase_dom.
DR SMART; SM00471; HDc; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Complete proteome;
KW Hydrolase; Manganese; Metal-binding; Reference proteome.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 179 Guanosine-3',5'-bis(diphosphate) 3'-
FT pyrophosphohydrolase MESH1.
FT /FTId=PRO_0000263110.
FT DOMAIN 32 127 HD.
FT ACT_SITE 65 65 Nucleophile (Probable).
FT ACT_SITE 66 66 Nucleophile (Probable).
FT METAL 35 35 Manganese.
FT METAL 61 61 Manganese.
FT METAL 62 62 Manganese.
FT METAL 122 122 Manganese.
FT MOD_RES 2 2 N-acetylglycine.
FT MOD_RES 25 25 N6-acetyllysine.
FT MOD_RES 97 97 N6-acetyllysine.
FT MOD_RES 123 123 N6-acetyllysine.
FT VAR_SEQ 137 140 GWSE -> VKIQ (in isoform 2).
FT /FTId=VSP_021866.
FT VAR_SEQ 141 179 Missing (in isoform 2).
FT /FTId=VSP_021867.
FT MUTAGEN 24 24 R->A: Abrogates ppGpp hydrolase activity.
FT MUTAGEN 65 65 E->A: Abrogates ppGpp hydrolase activity.
FT MUTAGEN 66 66 D->A: Reduces ppGpp hydrolase activity.
FT HELIX 4 18
FT TURN 19 21
FT HELIX 34 45
FT HELIX 52 60
FT HELIX 63 66
FT HELIX 71 88
FT HELIX 97 107
FT HELIX 108 110
FT HELIX 113 132
FT HELIX 140 157
FT HELIX 162 173
FT TURN 174 176
SQ SEQUENCE 179 AA; 20329 MW; E0946B0FA7BD8109 CRC64;
MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL
HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL
ADKLYNLRDL NRCTPEGWSE HRVQEYFEWA AQVVKGLQGT NRQLEEALKH LFKQRGLTI
//