Full text data of RABIF
RABIF
(MSS4, RASGRF3)
[Confidence: low (only semi-automatic identification from reviews)]
Guanine nucleotide exchange factor MSS4 (Rab-interacting factor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Guanine nucleotide exchange factor MSS4 (Rab-interacting factor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
P47224
ID MSS4_HUMAN Reviewed; 123 AA.
AC P47224; B2R4P4; Q92992;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1997, sequence version 2.
DT 22-JAN-2014, entry version 117.
DE RecName: Full=Guanine nucleotide exchange factor MSS4;
DE AltName: Full=Rab-interacting factor;
GN Name=RABIF; Synonyms=MSS4, RASGRF3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=7619808; DOI=10.1021/bi00028a020;
RA Yu H., Schreiber S.L.;
RT "Cloning, Zn2+ binding, and structural characterization of the guanine
RT nucleotide exchange factor human Mss4.";
RL Biochemistry 34:9103-9110(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9441742; DOI=10.1006/geno.1997.5049;
RA Mueller-Pillasch F., Zimmerhackl F., Lacher U., Schultz N.,
RA Hameister H., Varga G., Friess H., Buechler M., Adler G., Gress T.M.;
RT "Cloning of novel transcripts of the human guanine-nucleotide-exchange
RT factor Mss4: in situ chromosomal mapping and expression in pancreatic
RT cancer.";
RL Genomics 46:389-396(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP STRUCTURE BY NMR.
RX PubMed=7651540; DOI=10.1038/376788a0;
RA Yu H., Schreiber S.L.;
RT "Structure of guanine-nucleotide-exchange factor human Mss4 and
RT identification of its Rab-interacting surface.";
RL Nature 376:788-791(1995).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 11-123 IN COMPLEX WITH RAB8A,
RP AND INTERACTION WITH RAB8A.
RX PubMed=16541104; DOI=10.1038/sj.emboj.7601044;
RA Itzen A., Pylypenko O., Goody R.S., Alexandrov K., Rak A.;
RT "Nucleotide exchange via local protein unfolding--structure of Rab8 in
RT complex with MSS4.";
RL EMBO J. 25:1445-1455(2006).
CC -!- FUNCTION: Guanine-nucleotide-releasing protein that acts on
CC members of the SEC4/YPT1/RAB subfamily. Stimulates GDP release
CC from both YPT1 and RAB3A, but is less active on these proteins
CC than on the SEC4 protein. Might play a general role in vesicular
CC transport.
CC -!- SUBUNIT: Interacts with RAB8A.
CC -!- INTERACTION:
CC P61006:RAB8A; NbExp=2; IntAct=EBI-713992, EBI-722293;
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the DSS4/MSS4 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; S78873; AAB34955.1; -; mRNA.
DR EMBL; U74324; AAB18264.1; -; mRNA.
DR EMBL; BT007133; AAP35797.1; -; mRNA.
DR EMBL; AK311900; BAG34841.1; -; mRNA.
DR EMBL; CH471067; EAW91443.1; -; Genomic_DNA.
DR EMBL; BC018488; AAH18488.1; -; mRNA.
DR EMBL; BC037392; AAH37392.1; -; mRNA.
DR PIR; I52427; I52427.
DR RefSeq; NP_002862.2; NM_002871.4.
DR UniGene; Hs.90875; -.
DR PDB; 1FWQ; NMR; -; A=1-123.
DR PDB; 2FU5; X-ray; 2.00 A; A/B=11-123.
DR PDBsum; 1FWQ; -.
DR PDBsum; 2FU5; -.
DR ProteinModelPortal; P47224; -.
DR SMR; P47224; 9-123.
DR IntAct; P47224; 6.
DR MINT; MINT-1396805; -.
DR STRING; 9606.ENSP00000356231; -.
DR DMDM; 2506456; -.
DR PaxDb; P47224; -.
DR PRIDE; P47224; -.
DR DNASU; 5877; -.
DR Ensembl; ENST00000367262; ENSP00000356231; ENSG00000183155.
DR GeneID; 5877; -.
DR KEGG; hsa:5877; -.
DR UCSC; uc001gyl.3; human.
DR CTD; 5877; -.
DR GeneCards; GC01M202848; -.
DR HGNC; HGNC:9797; RABIF.
DR HPA; HPA027715; -.
DR MIM; 603417; gene.
DR neXtProt; NX_P47224; -.
DR PharmGKB; PA34158; -.
DR eggNOG; NOG239571; -.
DR HOGENOM; HOG000007903; -.
DR HOVERGEN; HBG006402; -.
DR InParanoid; P47224; -.
DR OMA; HWFVDDM; -.
DR OrthoDB; EOG76DTVD; -.
DR PhylomeDB; P47224; -.
DR EvolutionaryTrace; P47224; -.
DR GeneWiki; RABIF; -.
DR GenomeRNAi; 5877; -.
DR NextBio; 22836; -.
DR PRO; PR:P47224; -.
DR Bgee; P47224; -.
DR CleanEx; HS_RABIF; -.
DR Genevestigator; P47224; -.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; NAS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
DR GO; GO:0061025; P:membrane fusion; TAS:ProtInc.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 2.170.150.10; -; 1.
DR InterPro; IPR007515; Mss4.
DR InterPro; IPR011057; Mss4-like.
DR InterPro; IPR011323; Mss4/transl-control_tumor.
DR Pfam; PF04421; Mss4; 1.
DR SUPFAM; SSF51316; SSF51316; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Complete proteome;
KW Guanine-nucleotide releasing factor; Metal-binding; Protein transport;
KW Reference proteome; Transport; Zinc.
FT CHAIN 1 123 Guanine nucleotide exchange factor MSS4.
FT /FTId=PRO_0000174174.
FT METAL 23 23 Zinc.
FT METAL 26 26 Zinc.
FT METAL 94 94 Zinc.
FT METAL 97 97 Zinc.
FT MOD_RES 1 1 N-acetylmethionine.
FT CONFLICT 5 6 EQ -> DE (in Ref. 1; AAB34955).
FT STRAND 15 22
FT TURN 24 26
FT STRAND 29 31
FT STRAND 37 39
FT STRAND 43 46
FT STRAND 62 65
FT STRAND 68 72
FT HELIX 74 76
FT STRAND 78 83
FT STRAND 89 93
FT TURN 95 97
FT STRAND 103 106
FT STRAND 113 116
FT HELIX 117 119
FT STRAND 120 123
SQ SEQUENCE 123 AA; 13839 MW; 78E98395FAE10257 CRC64;
MEPAEQPSEL VSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP
DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV
SHE
//
ID MSS4_HUMAN Reviewed; 123 AA.
AC P47224; B2R4P4; Q92992;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1997, sequence version 2.
DT 22-JAN-2014, entry version 117.
DE RecName: Full=Guanine nucleotide exchange factor MSS4;
DE AltName: Full=Rab-interacting factor;
GN Name=RABIF; Synonyms=MSS4, RASGRF3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=7619808; DOI=10.1021/bi00028a020;
RA Yu H., Schreiber S.L.;
RT "Cloning, Zn2+ binding, and structural characterization of the guanine
RT nucleotide exchange factor human Mss4.";
RL Biochemistry 34:9103-9110(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9441742; DOI=10.1006/geno.1997.5049;
RA Mueller-Pillasch F., Zimmerhackl F., Lacher U., Schultz N.,
RA Hameister H., Varga G., Friess H., Buechler M., Adler G., Gress T.M.;
RT "Cloning of novel transcripts of the human guanine-nucleotide-exchange
RT factor Mss4: in situ chromosomal mapping and expression in pancreatic
RT cancer.";
RL Genomics 46:389-396(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP STRUCTURE BY NMR.
RX PubMed=7651540; DOI=10.1038/376788a0;
RA Yu H., Schreiber S.L.;
RT "Structure of guanine-nucleotide-exchange factor human Mss4 and
RT identification of its Rab-interacting surface.";
RL Nature 376:788-791(1995).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 11-123 IN COMPLEX WITH RAB8A,
RP AND INTERACTION WITH RAB8A.
RX PubMed=16541104; DOI=10.1038/sj.emboj.7601044;
RA Itzen A., Pylypenko O., Goody R.S., Alexandrov K., Rak A.;
RT "Nucleotide exchange via local protein unfolding--structure of Rab8 in
RT complex with MSS4.";
RL EMBO J. 25:1445-1455(2006).
CC -!- FUNCTION: Guanine-nucleotide-releasing protein that acts on
CC members of the SEC4/YPT1/RAB subfamily. Stimulates GDP release
CC from both YPT1 and RAB3A, but is less active on these proteins
CC than on the SEC4 protein. Might play a general role in vesicular
CC transport.
CC -!- SUBUNIT: Interacts with RAB8A.
CC -!- INTERACTION:
CC P61006:RAB8A; NbExp=2; IntAct=EBI-713992, EBI-722293;
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the DSS4/MSS4 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; S78873; AAB34955.1; -; mRNA.
DR EMBL; U74324; AAB18264.1; -; mRNA.
DR EMBL; BT007133; AAP35797.1; -; mRNA.
DR EMBL; AK311900; BAG34841.1; -; mRNA.
DR EMBL; CH471067; EAW91443.1; -; Genomic_DNA.
DR EMBL; BC018488; AAH18488.1; -; mRNA.
DR EMBL; BC037392; AAH37392.1; -; mRNA.
DR PIR; I52427; I52427.
DR RefSeq; NP_002862.2; NM_002871.4.
DR UniGene; Hs.90875; -.
DR PDB; 1FWQ; NMR; -; A=1-123.
DR PDB; 2FU5; X-ray; 2.00 A; A/B=11-123.
DR PDBsum; 1FWQ; -.
DR PDBsum; 2FU5; -.
DR ProteinModelPortal; P47224; -.
DR SMR; P47224; 9-123.
DR IntAct; P47224; 6.
DR MINT; MINT-1396805; -.
DR STRING; 9606.ENSP00000356231; -.
DR DMDM; 2506456; -.
DR PaxDb; P47224; -.
DR PRIDE; P47224; -.
DR DNASU; 5877; -.
DR Ensembl; ENST00000367262; ENSP00000356231; ENSG00000183155.
DR GeneID; 5877; -.
DR KEGG; hsa:5877; -.
DR UCSC; uc001gyl.3; human.
DR CTD; 5877; -.
DR GeneCards; GC01M202848; -.
DR HGNC; HGNC:9797; RABIF.
DR HPA; HPA027715; -.
DR MIM; 603417; gene.
DR neXtProt; NX_P47224; -.
DR PharmGKB; PA34158; -.
DR eggNOG; NOG239571; -.
DR HOGENOM; HOG000007903; -.
DR HOVERGEN; HBG006402; -.
DR InParanoid; P47224; -.
DR OMA; HWFVDDM; -.
DR OrthoDB; EOG76DTVD; -.
DR PhylomeDB; P47224; -.
DR EvolutionaryTrace; P47224; -.
DR GeneWiki; RABIF; -.
DR GenomeRNAi; 5877; -.
DR NextBio; 22836; -.
DR PRO; PR:P47224; -.
DR Bgee; P47224; -.
DR CleanEx; HS_RABIF; -.
DR Genevestigator; P47224; -.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; NAS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
DR GO; GO:0061025; P:membrane fusion; TAS:ProtInc.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 2.170.150.10; -; 1.
DR InterPro; IPR007515; Mss4.
DR InterPro; IPR011057; Mss4-like.
DR InterPro; IPR011323; Mss4/transl-control_tumor.
DR Pfam; PF04421; Mss4; 1.
DR SUPFAM; SSF51316; SSF51316; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Complete proteome;
KW Guanine-nucleotide releasing factor; Metal-binding; Protein transport;
KW Reference proteome; Transport; Zinc.
FT CHAIN 1 123 Guanine nucleotide exchange factor MSS4.
FT /FTId=PRO_0000174174.
FT METAL 23 23 Zinc.
FT METAL 26 26 Zinc.
FT METAL 94 94 Zinc.
FT METAL 97 97 Zinc.
FT MOD_RES 1 1 N-acetylmethionine.
FT CONFLICT 5 6 EQ -> DE (in Ref. 1; AAB34955).
FT STRAND 15 22
FT TURN 24 26
FT STRAND 29 31
FT STRAND 37 39
FT STRAND 43 46
FT STRAND 62 65
FT STRAND 68 72
FT HELIX 74 76
FT STRAND 78 83
FT STRAND 89 93
FT TURN 95 97
FT STRAND 103 106
FT STRAND 113 116
FT HELIX 117 119
FT STRAND 120 123
SQ SEQUENCE 123 AA; 13839 MW; 78E98395FAE10257 CRC64;
MEPAEQPSEL VSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP
DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV
SHE
//
MIM
603417
*RECORD*
*FIELD* NO
603417
*FIELD* TI
*603417 RAB-INTERACTING FACTOR; RABIF
;;RAS-SPECIFIC GUANINE-RELEASING FACTOR 3; RASGRF3;;
read moreMAMMALIAN SUPPRESSOR OF SEC4; MSS4
*FIELD* TX
DESCRIPTION
The Sec4/Rab-related small GTP-binding proteins are involved in the
regulation of intracellular vesicular transport. Mss4 stimulates GTP-GDP
exchange in Sec4 and Rab and binds to a subset of genetically related
Rab proteins.
CLONING
Yu and Schreiber (1995) cloned a human MSS4 cDNA. The gene encodes a
123-amino acid polypeptide that requires zinc for stability.
Muller-Pillasch et al. (1997) showed by Northern blot analysis that MSS4
is expressed as 3 differently sized mRNAs, probably due to alternative
polyadenylation signals. The transcripts are present at barely
detectable levels in healthy pancreas, but at much higher levels in
pancreatic and other cancer tissues.
GENE FUNCTION
Wixler et al. (1999) identified MSS4 as a binding partner of integrin
alpha-3 (605025) in a yeast 2-hybrid screen of a placenta cDNA library.
Using the conserved C-terminal motif of several other alpha-integrin
subunits, they found that MSS4 bound specifically to integrins
containing an aromatic amino acid before the first lysine of the
conserved motif. These integrins included alpha-2 (192974), alpha-3A,
alpha-3B, alpha-5 (135620), and alpha-6A (147556).
MAPPING
Muller-Pillasch et al. (1997) used fluorescence in situ hybridization to
map the MSS4 gene to human chromosome 1q32-q41.
*FIELD* RF
1. Muller-Pillasch, F.; Zimmerhackl, F.; Lacher, U.; Schultz, N.;
Hameister, H.; Varga, G.; Friess, H.; Buchler, M.; Adler, G.; Gress,
T. M.: Cloning of novel transcripts of the human guanine-nucleotide-exchange
factor Mss4: in situ chromosomal mapping and expression in pancreatic
cancer. Genomics 46: 389-396, 1997.
2. Wixler, V.; Laplantine, E.; Geerts, D.; Sonnenberg, A.; Petersohn,
D.; Eckes, B.; Paulsson, M.; Aumailley, M.: Identification of novel
interaction partners for the conserved membrane proximal region of
alpha-integrin cytoplasmic domains. FEBS Lett. 445: 351-355, 1999.
3. Yu, H.; Schreiber, S. L.: Cloning, Zn(2+) binding, and structural
characterization of the guanine nucleotide exchange factor human Mss4. Biochemistry 34:
9103-9110, 1995.
*FIELD* CN
Patricia A. Hartz - updated: 3/11/2003
*FIELD* CD
Jennifer P. Macke: 1/11/1999
*FIELD* ED
mgross: 03/13/2003
terry: 3/11/2003
alopez: 1/11/1999
*RECORD*
*FIELD* NO
603417
*FIELD* TI
*603417 RAB-INTERACTING FACTOR; RABIF
;;RAS-SPECIFIC GUANINE-RELEASING FACTOR 3; RASGRF3;;
read moreMAMMALIAN SUPPRESSOR OF SEC4; MSS4
*FIELD* TX
DESCRIPTION
The Sec4/Rab-related small GTP-binding proteins are involved in the
regulation of intracellular vesicular transport. Mss4 stimulates GTP-GDP
exchange in Sec4 and Rab and binds to a subset of genetically related
Rab proteins.
CLONING
Yu and Schreiber (1995) cloned a human MSS4 cDNA. The gene encodes a
123-amino acid polypeptide that requires zinc for stability.
Muller-Pillasch et al. (1997) showed by Northern blot analysis that MSS4
is expressed as 3 differently sized mRNAs, probably due to alternative
polyadenylation signals. The transcripts are present at barely
detectable levels in healthy pancreas, but at much higher levels in
pancreatic and other cancer tissues.
GENE FUNCTION
Wixler et al. (1999) identified MSS4 as a binding partner of integrin
alpha-3 (605025) in a yeast 2-hybrid screen of a placenta cDNA library.
Using the conserved C-terminal motif of several other alpha-integrin
subunits, they found that MSS4 bound specifically to integrins
containing an aromatic amino acid before the first lysine of the
conserved motif. These integrins included alpha-2 (192974), alpha-3A,
alpha-3B, alpha-5 (135620), and alpha-6A (147556).
MAPPING
Muller-Pillasch et al. (1997) used fluorescence in situ hybridization to
map the MSS4 gene to human chromosome 1q32-q41.
*FIELD* RF
1. Muller-Pillasch, F.; Zimmerhackl, F.; Lacher, U.; Schultz, N.;
Hameister, H.; Varga, G.; Friess, H.; Buchler, M.; Adler, G.; Gress,
T. M.: Cloning of novel transcripts of the human guanine-nucleotide-exchange
factor Mss4: in situ chromosomal mapping and expression in pancreatic
cancer. Genomics 46: 389-396, 1997.
2. Wixler, V.; Laplantine, E.; Geerts, D.; Sonnenberg, A.; Petersohn,
D.; Eckes, B.; Paulsson, M.; Aumailley, M.: Identification of novel
interaction partners for the conserved membrane proximal region of
alpha-integrin cytoplasmic domains. FEBS Lett. 445: 351-355, 1999.
3. Yu, H.; Schreiber, S. L.: Cloning, Zn(2+) binding, and structural
characterization of the guanine nucleotide exchange factor human Mss4. Biochemistry 34:
9103-9110, 1995.
*FIELD* CN
Patricia A. Hartz - updated: 3/11/2003
*FIELD* CD
Jennifer P. Macke: 1/11/1999
*FIELD* ED
mgross: 03/13/2003
terry: 3/11/2003
alopez: 1/11/1999