Full text data of MYL6
MYL6
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Myosin light polypeptide 6 (17 kDa myosin light chain; LC17; Myosin light chain 3; MLC-3; Myosin light chain alkali 3; Myosin light chain A3; Smooth muscle and nonmuscle myosin light chain alkali 6)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Myosin light polypeptide 6 (17 kDa myosin light chain; LC17; Myosin light chain 3; MLC-3; Myosin light chain alkali 3; Myosin light chain A3; Smooth muscle and nonmuscle myosin light chain alkali 6)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
P60660
ID MYL6_HUMAN Reviewed; 151 AA.
AC P60660; P16475; P24572; P24573; Q12790; Q561V9; Q6IAZ0; Q6IPY5;
read moreDT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 22-JAN-2014, entry version 105.
DE RecName: Full=Myosin light polypeptide 6;
DE AltName: Full=17 kDa myosin light chain;
DE Short=LC17;
DE AltName: Full=Myosin light chain 3;
DE Short=MLC-3;
DE AltName: Full=Myosin light chain alkali 3;
DE Short=Myosin light chain A3;
DE AltName: Full=Smooth muscle and nonmuscle myosin light chain alkali 6;
GN Name=MYL6;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS NON-MUSCLE AND
RP SMOOTH MUSCLE).
RX PubMed=2722814;
RA Lenz S., Lohse P., Seidel U., Arnold H.H.;
RT "The alkali light chains of human smooth and nonmuscle myosins are
RT encoded by a single gene. Tissue-specific expression by alternative
RT splicing pathways.";
RL J. Biol. Chem. 264:9009-9015(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
RX PubMed=2304459;
RA Hailstones D.L., Gunning P.W.;
RT "Characterization of human myosin light chains 1sa and 3nm:
RT implications for isoform evolution and function.";
RL Mol. Cell. Biol. 10:1095-1104(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
RC TISSUE=Eye;
RX PubMed=8188229; DOI=10.1006/geno.1994.1041;
RA Bora P.S., Bora N.S., Wu X., Kaplan H.J., Lange L.G.;
RT "Molecular cloning, sequencing, and characterization of smooth muscle
RT myosin alkali light chain from human eye cDNA: homology with
RT myocardial fatty acid ethyl ester synthase-III cDNA.";
RL Genomics 19:186-188(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
RX PubMed=11436981; DOI=10.1540/jsmr.37.25;
RA Watanabe M., Kohri M., Takaishi M., Horie R., Higashihara M.;
RT "Molecular cloning and sequencing of myosin light chains in human
RT megakaryoblastic leukemia cells.";
RL J. Smooth Muscle Res. 37:25-38(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-MUSCLE).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS NON-MUSCLE AND SMOOTH
RP MUSCLE).
RC TISSUE=Brain, Duodenum, Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 14-21; 38-50; 64-94 AND 111-119, AND MASS
RP SPECTROMETRY.
RC TISSUE=B-cell lymphoma;
RA Bienvenut W.V.;
RL Submitted (MAR-2005) to UniProtKB.
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 (ISOFORM SMOOTH
RP MUSCLE), AND MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-81, AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- INTERACTION:
CC P03372:ESR1; NbExp=3; IntAct=EBI-300817, EBI-78473;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Non-muscle; Synonyms=MLC3nm, LC17A, LC17-nm;
CC IsoId=P60660-1, P16475-1;
CC Sequence=Displayed;
CC Name=Smooth muscle; Synonyms=MLC3sm, LC17B, LC17-sm;
CC IsoId=P60660-2, P24572-2;
CC Sequence=VSP_009735;
CC Note=Contains a phosphoserine at position 135;
CC -!- SIMILARITY: Contains 3 EF-hand domains.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M22918; AAA36347.1; -; mRNA.
DR EMBL; M22919; AAA59892.1; -; Genomic_DNA.
DR EMBL; M22919; AAA59893.1; -; Genomic_DNA.
DR EMBL; M22920; AAA36348.1; -; mRNA.
DR EMBL; M31212; AAA59853.1; -; mRNA.
DR EMBL; U02629; AAA20643.1; -; mRNA.
DR EMBL; AB046613; BAB62402.1; -; mRNA.
DR EMBL; CR457014; CAG33295.1; -; mRNA.
DR EMBL; BC006781; AAH06781.2; -; mRNA.
DR EMBL; BC017455; AAH17455.1; -; mRNA.
DR EMBL; BC071661; AAH71661.1; -; mRNA.
DR EMBL; BC093066; AAH93066.1; -; mRNA.
DR PIR; A33709; MOHU6N.
DR PIR; A49620; MOHU6E.
DR PIR; B33709; MOHU6M.
DR RefSeq; NP_066299.2; NM_021019.4.
DR RefSeq; NP_524147.2; NM_079423.3.
DR RefSeq; XP_005268935.1; XM_005268878.1.
DR RefSeq; XP_005268936.1; XM_005268879.1.
DR RefSeq; XP_005268937.1; XM_005268880.1.
DR RefSeq; XP_005268939.1; XM_005268882.1.
DR UniGene; Hs.632717; -.
DR ProteinModelPortal; P60660; -.
DR SMR; P60660; 4-151.
DR IntAct; P60660; 24.
DR MINT; MINT-1131667; -.
DR STRING; 9606.ENSP00000293422; -.
DR PhosphoSite; P60660; -.
DR DMDM; 47606436; -.
DR DOSAC-COBS-2DPAGE; P60660; -.
DR SWISS-2DPAGE; P60660; -.
DR PaxDb; P60660; -.
DR PRIDE; P60660; -.
DR DNASU; 4637; -.
DR Ensembl; ENST00000547649; ENSP00000446714; ENSG00000092841.
DR Ensembl; ENST00000548293; ENSP00000448101; ENSG00000092841.
DR Ensembl; ENST00000550697; ENSP00000446955; ENSG00000092841.
DR GeneID; 4637; -.
DR KEGG; hsa:4637; -.
DR UCSC; uc001sjw.2; human.
DR CTD; 4637; -.
DR GeneCards; GC12P056552; -.
DR HGNC; HGNC:7587; MYL6.
DR HPA; HPA046859; -.
DR MIM; 609931; gene.
DR neXtProt; NX_P60660; -.
DR PharmGKB; PA31384; -.
DR eggNOG; COG5126; -.
DR HOVERGEN; HBG012180; -.
DR InParanoid; P60660; -.
DR KO; K12751; -.
DR OrthoDB; EOG7HQN9N; -.
DR Reactome; REACT_111045; Developmental Biology.
DR Reactome; REACT_17044; Muscle contraction.
DR ChiTaRS; MYL6; human.
DR GeneWiki; MYL6; -.
DR GenomeRNAi; 4637; -.
DR NextBio; 17854; -.
DR PRO; PR:P60660; -.
DR ArrayExpress; P60660; -.
DR Bgee; P60660; -.
DR CleanEx; HS_MYL6; -.
DR Genevestigator; P60660; -.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0016461; C:unconventional myosin complex; TAS:BHF-UCL.
DR GO; GO:0030898; F:actin-dependent ATPase activity; ISS:HGNC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0003774; F:motor activity; TAS:HGNC.
DR GO; GO:0008307; F:structural constituent of muscle; IDA:HGNC.
DR GO; GO:0007411; P:axon guidance; TAS:Reactome.
DR GO; GO:0030049; P:muscle filament sliding; TAS:HGNC.
DR GO; GO:0007519; P:skeletal muscle tissue development; TAS:UniProtKB.
DR Gene3D; 1.10.238.10; -; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13405; EF-hand_6; 1.
DR SMART; SM00054; EFh; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Complete proteome;
KW Direct protein sequencing; Motor protein; Muscle protein; Myosin;
KW Phosphoprotein; Polymorphism; Reference proteome; Repeat.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 151 Myosin light polypeptide 6.
FT /FTId=PRO_0000198690.
FT DOMAIN 7 42 EF-hand 1.
FT DOMAIN 84 119 EF-hand 2.
FT DOMAIN 119 151 EF-hand 3.
FT MOD_RES 2 2 N-acetylcysteine.
FT MOD_RES 81 81 N6-acetyllysine.
FT VAR_SEQ 143 151 AFVRHILSG -> ELVRMVLNG (in isoform Smooth
FT muscle).
FT /FTId=VSP_009735.
FT VARIANT 85 85 T -> I (in dbSNP:rs11553509).
FT /FTId=VAR_050457.
FT VARIANT 103 103 T -> P (in dbSNP:rs1050470).
FT /FTId=VAR_034118.
FT CONFLICT 10 10 A -> ADLIPST (in Ref. 3; AAA20643).
FT CONFLICT 45 45 N -> D (in Ref. 2; AAA59853).
FT CONFLICT 76 76 T -> R (in Ref. 3; AAA20643).
SQ SEQUENCE 151 AA; 16930 MW; A2B7B4F41179523D CRC64;
MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEM
NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG NGTVMGAEIR HVLVTLGEKM
TEEEVEMLVA GHEDSNGCIN YEAFVRHILS G
//
ID MYL6_HUMAN Reviewed; 151 AA.
AC P60660; P16475; P24572; P24573; Q12790; Q561V9; Q6IAZ0; Q6IPY5;
read moreDT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 22-JAN-2014, entry version 105.
DE RecName: Full=Myosin light polypeptide 6;
DE AltName: Full=17 kDa myosin light chain;
DE Short=LC17;
DE AltName: Full=Myosin light chain 3;
DE Short=MLC-3;
DE AltName: Full=Myosin light chain alkali 3;
DE Short=Myosin light chain A3;
DE AltName: Full=Smooth muscle and nonmuscle myosin light chain alkali 6;
GN Name=MYL6;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS NON-MUSCLE AND
RP SMOOTH MUSCLE).
RX PubMed=2722814;
RA Lenz S., Lohse P., Seidel U., Arnold H.H.;
RT "The alkali light chains of human smooth and nonmuscle myosins are
RT encoded by a single gene. Tissue-specific expression by alternative
RT splicing pathways.";
RL J. Biol. Chem. 264:9009-9015(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
RX PubMed=2304459;
RA Hailstones D.L., Gunning P.W.;
RT "Characterization of human myosin light chains 1sa and 3nm:
RT implications for isoform evolution and function.";
RL Mol. Cell. Biol. 10:1095-1104(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
RC TISSUE=Eye;
RX PubMed=8188229; DOI=10.1006/geno.1994.1041;
RA Bora P.S., Bora N.S., Wu X., Kaplan H.J., Lange L.G.;
RT "Molecular cloning, sequencing, and characterization of smooth muscle
RT myosin alkali light chain from human eye cDNA: homology with
RT myocardial fatty acid ethyl ester synthase-III cDNA.";
RL Genomics 19:186-188(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM NON-MUSCLE).
RX PubMed=11436981; DOI=10.1540/jsmr.37.25;
RA Watanabe M., Kohri M., Takaishi M., Horie R., Higashihara M.;
RT "Molecular cloning and sequencing of myosin light chains in human
RT megakaryoblastic leukemia cells.";
RL J. Smooth Muscle Res. 37:25-38(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-MUSCLE).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS NON-MUSCLE AND SMOOTH
RP MUSCLE).
RC TISSUE=Brain, Duodenum, Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 14-21; 38-50; 64-94 AND 111-119, AND MASS
RP SPECTROMETRY.
RC TISSUE=B-cell lymphoma;
RA Bienvenut W.V.;
RL Submitted (MAR-2005) to UniProtKB.
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 (ISOFORM SMOOTH
RP MUSCLE), AND MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-81, AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT CYS-2, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Regulatory light chain of myosin. Does not bind calcium.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- INTERACTION:
CC P03372:ESR1; NbExp=3; IntAct=EBI-300817, EBI-78473;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Non-muscle; Synonyms=MLC3nm, LC17A, LC17-nm;
CC IsoId=P60660-1, P16475-1;
CC Sequence=Displayed;
CC Name=Smooth muscle; Synonyms=MLC3sm, LC17B, LC17-sm;
CC IsoId=P60660-2, P24572-2;
CC Sequence=VSP_009735;
CC Note=Contains a phosphoserine at position 135;
CC -!- SIMILARITY: Contains 3 EF-hand domains.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M22918; AAA36347.1; -; mRNA.
DR EMBL; M22919; AAA59892.1; -; Genomic_DNA.
DR EMBL; M22919; AAA59893.1; -; Genomic_DNA.
DR EMBL; M22920; AAA36348.1; -; mRNA.
DR EMBL; M31212; AAA59853.1; -; mRNA.
DR EMBL; U02629; AAA20643.1; -; mRNA.
DR EMBL; AB046613; BAB62402.1; -; mRNA.
DR EMBL; CR457014; CAG33295.1; -; mRNA.
DR EMBL; BC006781; AAH06781.2; -; mRNA.
DR EMBL; BC017455; AAH17455.1; -; mRNA.
DR EMBL; BC071661; AAH71661.1; -; mRNA.
DR EMBL; BC093066; AAH93066.1; -; mRNA.
DR PIR; A33709; MOHU6N.
DR PIR; A49620; MOHU6E.
DR PIR; B33709; MOHU6M.
DR RefSeq; NP_066299.2; NM_021019.4.
DR RefSeq; NP_524147.2; NM_079423.3.
DR RefSeq; XP_005268935.1; XM_005268878.1.
DR RefSeq; XP_005268936.1; XM_005268879.1.
DR RefSeq; XP_005268937.1; XM_005268880.1.
DR RefSeq; XP_005268939.1; XM_005268882.1.
DR UniGene; Hs.632717; -.
DR ProteinModelPortal; P60660; -.
DR SMR; P60660; 4-151.
DR IntAct; P60660; 24.
DR MINT; MINT-1131667; -.
DR STRING; 9606.ENSP00000293422; -.
DR PhosphoSite; P60660; -.
DR DMDM; 47606436; -.
DR DOSAC-COBS-2DPAGE; P60660; -.
DR SWISS-2DPAGE; P60660; -.
DR PaxDb; P60660; -.
DR PRIDE; P60660; -.
DR DNASU; 4637; -.
DR Ensembl; ENST00000547649; ENSP00000446714; ENSG00000092841.
DR Ensembl; ENST00000548293; ENSP00000448101; ENSG00000092841.
DR Ensembl; ENST00000550697; ENSP00000446955; ENSG00000092841.
DR GeneID; 4637; -.
DR KEGG; hsa:4637; -.
DR UCSC; uc001sjw.2; human.
DR CTD; 4637; -.
DR GeneCards; GC12P056552; -.
DR HGNC; HGNC:7587; MYL6.
DR HPA; HPA046859; -.
DR MIM; 609931; gene.
DR neXtProt; NX_P60660; -.
DR PharmGKB; PA31384; -.
DR eggNOG; COG5126; -.
DR HOVERGEN; HBG012180; -.
DR InParanoid; P60660; -.
DR KO; K12751; -.
DR OrthoDB; EOG7HQN9N; -.
DR Reactome; REACT_111045; Developmental Biology.
DR Reactome; REACT_17044; Muscle contraction.
DR ChiTaRS; MYL6; human.
DR GeneWiki; MYL6; -.
DR GenomeRNAi; 4637; -.
DR NextBio; 17854; -.
DR PRO; PR:P60660; -.
DR ArrayExpress; P60660; -.
DR Bgee; P60660; -.
DR CleanEx; HS_MYL6; -.
DR Genevestigator; P60660; -.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0016461; C:unconventional myosin complex; TAS:BHF-UCL.
DR GO; GO:0030898; F:actin-dependent ATPase activity; ISS:HGNC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0003774; F:motor activity; TAS:HGNC.
DR GO; GO:0008307; F:structural constituent of muscle; IDA:HGNC.
DR GO; GO:0007411; P:axon guidance; TAS:Reactome.
DR GO; GO:0030049; P:muscle filament sliding; TAS:HGNC.
DR GO; GO:0007519; P:skeletal muscle tissue development; TAS:UniProtKB.
DR Gene3D; 1.10.238.10; -; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13405; EF-hand_6; 1.
DR SMART; SM00054; EFh; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Complete proteome;
KW Direct protein sequencing; Motor protein; Muscle protein; Myosin;
KW Phosphoprotein; Polymorphism; Reference proteome; Repeat.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 151 Myosin light polypeptide 6.
FT /FTId=PRO_0000198690.
FT DOMAIN 7 42 EF-hand 1.
FT DOMAIN 84 119 EF-hand 2.
FT DOMAIN 119 151 EF-hand 3.
FT MOD_RES 2 2 N-acetylcysteine.
FT MOD_RES 81 81 N6-acetyllysine.
FT VAR_SEQ 143 151 AFVRHILSG -> ELVRMVLNG (in isoform Smooth
FT muscle).
FT /FTId=VSP_009735.
FT VARIANT 85 85 T -> I (in dbSNP:rs11553509).
FT /FTId=VAR_050457.
FT VARIANT 103 103 T -> P (in dbSNP:rs1050470).
FT /FTId=VAR_034118.
FT CONFLICT 10 10 A -> ADLIPST (in Ref. 3; AAA20643).
FT CONFLICT 45 45 N -> D (in Ref. 2; AAA59853).
FT CONFLICT 76 76 T -> R (in Ref. 3; AAA20643).
SQ SEQUENCE 151 AA; 16930 MW; A2B7B4F41179523D CRC64;
MCDFTEDQTA EFKEAFQLFD RTGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEM
NVKVLDFEHF LPMLQTVAKN KDQGTYEDYV EGLRVFDKEG NGTVMGAEIR HVLVTLGEKM
TEEEVEMLVA GHEDSNGCIN YEAFVRHILS G
//
MIM
609931
*RECORD*
*FIELD* NO
609931
*FIELD* TI
*609931 MYOSIN LIGHT CHAIN 6, ALKALI, SMOOTH MUSCLE AND NONMUSCLE; MYL6
*FIELD* TX
read more
DESCRIPTION
Myosin is composed of heavy chain (MHC; see 160730) and light chain
(MLC) subunits. Alkaline MLCs, such as MYL6, dissociate from MHCs at
high pH. MYL6 encodes both smooth muscle and nonmuscle MLC isoforms
(Hailstones and Gunning, 1990).
CLONING
By screening a genomic library with a chicken Mlc and a partial clone of
MYL1 (160780), followed by screening lymphoblast and smooth muscle cDNA
libraries, Lenz et al. (1989) cloned 2 MYL6 variants generated by
alternative splicing of exon 6. They designated the nonmuscle variant
LYM4 and the smooth muscle variant GT6. Both deduced proteins contain
151 amino acids and differ at only 5 C-terminal amino acids. The GT6
transcript also has a longer 3-prime UTR. Northern blot analysis
detected a 0.7-kb LYM4 transcript in both HeLa cells and human uterus
smooth muscle, whereas a GT6 transcript of similar size was detected in
uterus smooth muscle only.
By screening a transformed human fibroblast cDNA library with MLC1SA
(609930), followed by RT-PCR of human colon RNA, Hailstones and Gunning
(1990) cloned the nonmuscle and smooth muscle variants of MYL6, which
they designated MLC3NM and MLC3SM, respectively. Both deduced proteins
contain 151 amino acids and differ from other MLCs in that they lack
highly basic amino aids in their N termini. MLC3NM has a calculated
molecular mass of 16.9 kD, whereas MLC3SM has a calculated molecular
mass of 17 kD. Expression of MYL6 was highest in fibroblasts and
myoblasts, lower in fetal muscle, and lowest in adult muscle.
Using glutathione S-transferase-3 (GSTP1; 134660) as probe of a retina
cDNA library, Bora et al. (1994) cloned an MYL6 splice variant that they
designated ESMLC. The deduced 157-amino acid ESMLC protein is identical
to GT6 (Lenz et al., 1989) except that it contains additional N-terminal
amino acids. The nucleotide sequence of GSTP1 and ESMLC are 54%
identical, but the deduced proteins are only 11% identical. PCR analysis
detected ESMLC expression in eye, aorta, and heart.
GENE STRUCTURE
Lenz et al. (1989) determined that the MYL6 gene contains 7 exons. Exon
1 encodes only the first methionine and the 5-prime UTR.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the MYL6
gene to chromosome 12 (TMAP WI-18807).
*FIELD* RF
1. Bora, P. S.; Bora, N. S.; Wu, X.; Kaplan, H. J.; Lange, L. G.:
Molecular cloning, sequencing, and characterization of smooth muscle
myosin alkali light chain from human eye cDNA: homology with myocardial
fatty acid ethyl ester synthase-III cDNA. Genomics 19: 186-188,
1994.
2. Hailstones, D. L.; Gunning, P. W.: Characterization of human myosin
light chains 1sa and 3nm: implications for isoform evolution and function. Molec.
Cell. Biol. 10: 1095-1104, 1990.
3. Lenz, S.; Lohse, P.; Seidel, U.; Arnold, H.-H.: The alkali light
chains of human smooth and nonmuscle myosins are encoded by a single
gene: tissue-specific expression by alternative splicing pathways. J.
Biol. Chem. 264: 9009-9015, 1989.
*FIELD* CD
Patricia A. Hartz: 2/24/2006
*FIELD* ED
mgross: 02/24/2006
*RECORD*
*FIELD* NO
609931
*FIELD* TI
*609931 MYOSIN LIGHT CHAIN 6, ALKALI, SMOOTH MUSCLE AND NONMUSCLE; MYL6
*FIELD* TX
read more
DESCRIPTION
Myosin is composed of heavy chain (MHC; see 160730) and light chain
(MLC) subunits. Alkaline MLCs, such as MYL6, dissociate from MHCs at
high pH. MYL6 encodes both smooth muscle and nonmuscle MLC isoforms
(Hailstones and Gunning, 1990).
CLONING
By screening a genomic library with a chicken Mlc and a partial clone of
MYL1 (160780), followed by screening lymphoblast and smooth muscle cDNA
libraries, Lenz et al. (1989) cloned 2 MYL6 variants generated by
alternative splicing of exon 6. They designated the nonmuscle variant
LYM4 and the smooth muscle variant GT6. Both deduced proteins contain
151 amino acids and differ at only 5 C-terminal amino acids. The GT6
transcript also has a longer 3-prime UTR. Northern blot analysis
detected a 0.7-kb LYM4 transcript in both HeLa cells and human uterus
smooth muscle, whereas a GT6 transcript of similar size was detected in
uterus smooth muscle only.
By screening a transformed human fibroblast cDNA library with MLC1SA
(609930), followed by RT-PCR of human colon RNA, Hailstones and Gunning
(1990) cloned the nonmuscle and smooth muscle variants of MYL6, which
they designated MLC3NM and MLC3SM, respectively. Both deduced proteins
contain 151 amino acids and differ from other MLCs in that they lack
highly basic amino aids in their N termini. MLC3NM has a calculated
molecular mass of 16.9 kD, whereas MLC3SM has a calculated molecular
mass of 17 kD. Expression of MYL6 was highest in fibroblasts and
myoblasts, lower in fetal muscle, and lowest in adult muscle.
Using glutathione S-transferase-3 (GSTP1; 134660) as probe of a retina
cDNA library, Bora et al. (1994) cloned an MYL6 splice variant that they
designated ESMLC. The deduced 157-amino acid ESMLC protein is identical
to GT6 (Lenz et al., 1989) except that it contains additional N-terminal
amino acids. The nucleotide sequence of GSTP1 and ESMLC are 54%
identical, but the deduced proteins are only 11% identical. PCR analysis
detected ESMLC expression in eye, aorta, and heart.
GENE STRUCTURE
Lenz et al. (1989) determined that the MYL6 gene contains 7 exons. Exon
1 encodes only the first methionine and the 5-prime UTR.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the MYL6
gene to chromosome 12 (TMAP WI-18807).
*FIELD* RF
1. Bora, P. S.; Bora, N. S.; Wu, X.; Kaplan, H. J.; Lange, L. G.:
Molecular cloning, sequencing, and characterization of smooth muscle
myosin alkali light chain from human eye cDNA: homology with myocardial
fatty acid ethyl ester synthase-III cDNA. Genomics 19: 186-188,
1994.
2. Hailstones, D. L.; Gunning, P. W.: Characterization of human myosin
light chains 1sa and 3nm: implications for isoform evolution and function. Molec.
Cell. Biol. 10: 1095-1104, 1990.
3. Lenz, S.; Lohse, P.; Seidel, U.; Arnold, H.-H.: The alkali light
chains of human smooth and nonmuscle myosins are encoded by a single
gene: tissue-specific expression by alternative splicing pathways. J.
Biol. Chem. 264: 9009-9015, 1989.
*FIELD* CD
Patricia A. Hartz: 2/24/2006
*FIELD* ED
mgross: 02/24/2006