Full text data of NUP43
NUP43
[Confidence: low (only semi-automatic identification from reviews)]
Nucleoporin Nup43 (Nup107-160 subcomplex subunit Nup43; p42)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Nucleoporin Nup43 (Nup107-160 subcomplex subunit Nup43; p42)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q8NFH3
ID NUP43_HUMAN Reviewed; 380 AA.
AC Q8NFH3; Q9H8S0;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2002, sequence version 1.
DT 22-JAN-2014, entry version 104.
DE RecName: Full=Nucleoporin Nup43;
DE AltName: Full=Nup107-160 subcomplex subunit Nup43;
DE AltName: Full=p42;
GN Name=NUP43;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX PubMed=12196509; DOI=10.1083/jcb.200206106;
RA Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J.;
RT "Proteomic analysis of the mammalian nuclear pore complex.";
RL J. Cell Biol. 158:915-927(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 247-380.
RC TISSUE=Ovarian carcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP IDENTIFICATION IN THE NUP107-160 COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=15146057; DOI=10.1091/mbc.E03-12-0878;
RA Loieodice I., Alves A., Rabut G., Van Overbeek M., Ellenberg J.,
RA Sibarita J.-B., Doye V.;
RT "The entire Nup107-160 complex, including three new members, is
RT targeted as one entity to kinetochores in mitosis.";
RL Mol. Biol. Cell 15:3333-3344(2004).
RN [5]
RP FUNCTION OF THE NUP107-160 COMPLEX.
RX PubMed=17363900; DOI=10.1038/sj.emboj.7601642;
RA Zuccolo M., Alves A., Galy V., Bolhy S., Formstecher E., Racine V.,
RA Sibarita J.-B., Fukagawa T., Shiekhattar R., Yen T., Doye V.;
RT "The human Nup107-160 nuclear pore subcomplex contributes to proper
RT kinetochore functions.";
RL EMBO J. 26:1853-1864(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE
RP NUP107-160 COMPLEX.
RX PubMed=17360435; DOI=10.1073/pnas.0700058104;
RA Glavy J.S., Krutchinsky A.N., Cristea I.M., Berke I.C., Boehmer T.,
RA Blobel G., Chait B.T.;
RT "Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160
RT subcomplex.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:3811-3816(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the Nup107-160 subcomplex of the nuclear
CC pore complex (NPC). The Nup107-160 subcomplex is required for the
CC assembly of a functional NPC. The Nup107-160 subcomplex is also
CC required for normal kinetochore microtubule attachment, mitotic
CC progression and chromosome segregation.
CC -!- SUBUNIT: Component of the Nup107-160 subcomplex of the nuclear
CC pore complex (NPC). The Nup107-160 subcomplex includes NUP160,
CC NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore.
CC Nucleus, nuclear pore complex.
CC -!- SIMILARITY: Contains 6 WD repeats.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB14536.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF514997; AAM76708.1; -; mRNA.
DR EMBL; BC065028; AAH65028.1; -; mRNA.
DR EMBL; AK023349; BAB14536.1; ALT_INIT; mRNA.
DR RefSeq; NP_942590.1; NM_198887.2.
DR UniGene; Hs.510375; -.
DR PDB; 4I79; X-ray; 1.75 A; A/B=1-380.
DR PDBsum; 4I79; -.
DR ProteinModelPortal; Q8NFH3; -.
DR SMR; Q8NFH3; 4-380.
DR IntAct; Q8NFH3; 3.
DR MINT; MINT-4535305; -.
DR STRING; 9606.ENSP00000342262; -.
DR PhosphoSite; Q8NFH3; -.
DR DMDM; 27923819; -.
DR PaxDb; Q8NFH3; -.
DR PRIDE; Q8NFH3; -.
DR DNASU; 348995; -.
DR Ensembl; ENST00000340413; ENSP00000342262; ENSG00000120253.
DR Ensembl; ENST00000460354; ENSP00000432401; ENSG00000120253.
DR GeneID; 348995; -.
DR KEGG; hsa:348995; -.
DR UCSC; uc003qmz.3; human.
DR CTD; 348995; -.
DR GeneCards; GC06M150087; -.
DR HGNC; HGNC:21182; NUP43.
DR HPA; HPA027126; -.
DR HPA; HPA027291; -.
DR HPA; HPA028500; -.
DR MIM; 608141; gene.
DR neXtProt; NX_Q8NFH3; -.
DR PharmGKB; PA134930788; -.
DR eggNOG; NOG270547; -.
DR HOGENOM; HOG000007964; -.
DR HOVERGEN; HBG052696; -.
DR InParanoid; Q8NFH3; -.
DR KO; K14305; -.
DR OMA; QWTAAHY; -.
DR OrthoDB; EOG7Z3F4F; -.
DR PhylomeDB; Q8NFH3; -.
DR Reactome; REACT_111217; Metabolism.
DR Reactome; REACT_115566; Cell Cycle.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_15518; Transmembrane transport of small molecules.
DR Reactome; REACT_21300; Mitotic M-M/G1 phases.
DR Reactome; REACT_6900; Immune System.
DR SignaLink; Q8NFH3; -.
DR GeneWiki; NUP43; -.
DR GenomeRNAi; 348995; -.
DR NextBio; 99471; -.
DR PRO; PR:Q8NFH3; -.
DR ArrayExpress; Q8NFH3; -.
DR Bgee; Q8NFH3; -.
DR CleanEx; HS_NUP43; -.
DR Genevestigator; Q8NFH3; -.
DR GO; GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0031080; C:nuclear pore outer ring; IDA:UniProtKB.
DR GO; GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
DR GO; GO:0015758; P:glucose transport; TAS:Reactome.
DR GO; GO:0007067; P:mitosis; IEA:UniProtKB-KW.
DR GO; GO:0007077; P:mitotic nuclear envelope disassembly; TAS:Reactome.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0010827; P:regulation of glucose transport; TAS:Reactome.
DR GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR GO; GO:0055085; P:transmembrane transport; TAS:Reactome.
DR GO; GO:0016032; P:viral process; TAS:Reactome.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR017986; WD40_repeat_dom.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell cycle; Cell division; Centromere;
KW Chromosome; Chromosome partition; Complete proteome; Kinetochore;
KW Mitosis; mRNA transport; Nuclear pore complex; Nucleus;
KW Protein transport; Reference proteome; Repeat; Translocation;
KW Transport; WD repeat.
FT CHAIN 1 380 Nucleoporin Nup43.
FT /FTId=PRO_0000051111.
FT REPEAT 8 57 WD 1.
FT REPEAT 72 110 WD 2.
FT REPEAT 127 166 WD 3.
FT REPEAT 170 208 WD 4.
FT REPEAT 215 255 WD 5.
FT REPEAT 259 299 WD 6.
FT MOD_RES 1 1 N-acetylmethionine.
FT CONFLICT 247 247 V -> A (in Ref. 3; BAB14536).
FT STRAND 5 8
FT STRAND 10 18
FT STRAND 31 48
FT STRAND 66 75
FT STRAND 77 92
FT STRAND 97 102
FT TURN 104 106
FT STRAND 109 115
FT STRAND 118 120
FT STRAND 134 140
FT STRAND 143 148
FT STRAND 151 157
FT STRAND 164 168
FT STRAND 175 182
FT STRAND 185 190
FT STRAND 193 199
FT STRAND 203 205
FT STRAND 208 212
FT STRAND 214 217
FT STRAND 220 225
FT STRAND 232 237
FT STRAND 242 246
FT STRAND 254 257
FT STRAND 264 269
FT STRAND 276 281
FT STRAND 286 290
FT STRAND 341 344
FT STRAND 353 359
FT STRAND 362 367
FT STRAND 370 376
SQ SEQUENCE 380 AA; 42151 MW; EE44714B8E5BFB42 CRC64;
MEEIYAKFVS QKISKTRWRP LPPGSLQTAE TFATGSWDNE ENYISLWSIG DFGNLDSDGG
FEGDHQLLCD IRHHGDVMDL QFFDQERIVA ASSTGCVTVF LHHPNNQTLS VNQQWTTAHY
HTGPGSPSYS SAPCTGVVCN NPEIVTVGED GRINLFRADH KEAVRTIDNA DSSTLHAVTF
LRTPEILTVN SIGQLKIWDF RQQGNEPSQI LSLTGDRVPL HCVDRHPNQQ HVVATGGQDG
MLSIWDVRQG TMPVSLLKAH EAEMWEVHFH PSNPEHLFTC SEDGSLWHWD ASTDVPEKSS
LFHQGGRSST FLSHSISNQA NVHQSVISSW LSTDPAKDRI EITSLLPSRS LSVNTLDVLG
PCLVCGTDAE AIYVTRHLFS
//
ID NUP43_HUMAN Reviewed; 380 AA.
AC Q8NFH3; Q9H8S0;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2002, sequence version 1.
DT 22-JAN-2014, entry version 104.
DE RecName: Full=Nucleoporin Nup43;
DE AltName: Full=Nup107-160 subcomplex subunit Nup43;
DE AltName: Full=p42;
GN Name=NUP43;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RX PubMed=12196509; DOI=10.1083/jcb.200206106;
RA Cronshaw J.M., Krutchinsky A.N., Zhang W., Chait B.T., Matunis M.J.;
RT "Proteomic analysis of the mammalian nuclear pore complex.";
RL J. Cell Biol. 158:915-927(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 247-380.
RC TISSUE=Ovarian carcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP IDENTIFICATION IN THE NUP107-160 COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=15146057; DOI=10.1091/mbc.E03-12-0878;
RA Loieodice I., Alves A., Rabut G., Van Overbeek M., Ellenberg J.,
RA Sibarita J.-B., Doye V.;
RT "The entire Nup107-160 complex, including three new members, is
RT targeted as one entity to kinetochores in mitosis.";
RL Mol. Biol. Cell 15:3333-3344(2004).
RN [5]
RP FUNCTION OF THE NUP107-160 COMPLEX.
RX PubMed=17363900; DOI=10.1038/sj.emboj.7601642;
RA Zuccolo M., Alves A., Galy V., Bolhy S., Formstecher E., Racine V.,
RA Sibarita J.-B., Fukagawa T., Shiekhattar R., Yen T., Doye V.;
RT "The human Nup107-160 nuclear pore subcomplex contributes to proper
RT kinetochore functions.";
RL EMBO J. 26:1853-1864(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE
RP NUP107-160 COMPLEX.
RX PubMed=17360435; DOI=10.1073/pnas.0700058104;
RA Glavy J.S., Krutchinsky A.N., Cristea I.M., Berke I.C., Boehmer T.,
RA Blobel G., Chait B.T.;
RT "Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160
RT subcomplex.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:3811-3816(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the Nup107-160 subcomplex of the nuclear
CC pore complex (NPC). The Nup107-160 subcomplex is required for the
CC assembly of a functional NPC. The Nup107-160 subcomplex is also
CC required for normal kinetochore microtubule attachment, mitotic
CC progression and chromosome segregation.
CC -!- SUBUNIT: Component of the Nup107-160 subcomplex of the nuclear
CC pore complex (NPC). The Nup107-160 subcomplex includes NUP160,
CC NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore.
CC Nucleus, nuclear pore complex.
CC -!- SIMILARITY: Contains 6 WD repeats.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB14536.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF514997; AAM76708.1; -; mRNA.
DR EMBL; BC065028; AAH65028.1; -; mRNA.
DR EMBL; AK023349; BAB14536.1; ALT_INIT; mRNA.
DR RefSeq; NP_942590.1; NM_198887.2.
DR UniGene; Hs.510375; -.
DR PDB; 4I79; X-ray; 1.75 A; A/B=1-380.
DR PDBsum; 4I79; -.
DR ProteinModelPortal; Q8NFH3; -.
DR SMR; Q8NFH3; 4-380.
DR IntAct; Q8NFH3; 3.
DR MINT; MINT-4535305; -.
DR STRING; 9606.ENSP00000342262; -.
DR PhosphoSite; Q8NFH3; -.
DR DMDM; 27923819; -.
DR PaxDb; Q8NFH3; -.
DR PRIDE; Q8NFH3; -.
DR DNASU; 348995; -.
DR Ensembl; ENST00000340413; ENSP00000342262; ENSG00000120253.
DR Ensembl; ENST00000460354; ENSP00000432401; ENSG00000120253.
DR GeneID; 348995; -.
DR KEGG; hsa:348995; -.
DR UCSC; uc003qmz.3; human.
DR CTD; 348995; -.
DR GeneCards; GC06M150087; -.
DR HGNC; HGNC:21182; NUP43.
DR HPA; HPA027126; -.
DR HPA; HPA027291; -.
DR HPA; HPA028500; -.
DR MIM; 608141; gene.
DR neXtProt; NX_Q8NFH3; -.
DR PharmGKB; PA134930788; -.
DR eggNOG; NOG270547; -.
DR HOGENOM; HOG000007964; -.
DR HOVERGEN; HBG052696; -.
DR InParanoid; Q8NFH3; -.
DR KO; K14305; -.
DR OMA; QWTAAHY; -.
DR OrthoDB; EOG7Z3F4F; -.
DR PhylomeDB; Q8NFH3; -.
DR Reactome; REACT_111217; Metabolism.
DR Reactome; REACT_115566; Cell Cycle.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_15518; Transmembrane transport of small molecules.
DR Reactome; REACT_21300; Mitotic M-M/G1 phases.
DR Reactome; REACT_6900; Immune System.
DR SignaLink; Q8NFH3; -.
DR GeneWiki; NUP43; -.
DR GenomeRNAi; 348995; -.
DR NextBio; 99471; -.
DR PRO; PR:Q8NFH3; -.
DR ArrayExpress; Q8NFH3; -.
DR Bgee; Q8NFH3; -.
DR CleanEx; HS_NUP43; -.
DR Genevestigator; Q8NFH3; -.
DR GO; GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0031080; C:nuclear pore outer ring; IDA:UniProtKB.
DR GO; GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
DR GO; GO:0015758; P:glucose transport; TAS:Reactome.
DR GO; GO:0007067; P:mitosis; IEA:UniProtKB-KW.
DR GO; GO:0007077; P:mitotic nuclear envelope disassembly; TAS:Reactome.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0010827; P:regulation of glucose transport; TAS:Reactome.
DR GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR GO; GO:0055085; P:transmembrane transport; TAS:Reactome.
DR GO; GO:0016032; P:viral process; TAS:Reactome.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR017986; WD40_repeat_dom.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Cell cycle; Cell division; Centromere;
KW Chromosome; Chromosome partition; Complete proteome; Kinetochore;
KW Mitosis; mRNA transport; Nuclear pore complex; Nucleus;
KW Protein transport; Reference proteome; Repeat; Translocation;
KW Transport; WD repeat.
FT CHAIN 1 380 Nucleoporin Nup43.
FT /FTId=PRO_0000051111.
FT REPEAT 8 57 WD 1.
FT REPEAT 72 110 WD 2.
FT REPEAT 127 166 WD 3.
FT REPEAT 170 208 WD 4.
FT REPEAT 215 255 WD 5.
FT REPEAT 259 299 WD 6.
FT MOD_RES 1 1 N-acetylmethionine.
FT CONFLICT 247 247 V -> A (in Ref. 3; BAB14536).
FT STRAND 5 8
FT STRAND 10 18
FT STRAND 31 48
FT STRAND 66 75
FT STRAND 77 92
FT STRAND 97 102
FT TURN 104 106
FT STRAND 109 115
FT STRAND 118 120
FT STRAND 134 140
FT STRAND 143 148
FT STRAND 151 157
FT STRAND 164 168
FT STRAND 175 182
FT STRAND 185 190
FT STRAND 193 199
FT STRAND 203 205
FT STRAND 208 212
FT STRAND 214 217
FT STRAND 220 225
FT STRAND 232 237
FT STRAND 242 246
FT STRAND 254 257
FT STRAND 264 269
FT STRAND 276 281
FT STRAND 286 290
FT STRAND 341 344
FT STRAND 353 359
FT STRAND 362 367
FT STRAND 370 376
SQ SEQUENCE 380 AA; 42151 MW; EE44714B8E5BFB42 CRC64;
MEEIYAKFVS QKISKTRWRP LPPGSLQTAE TFATGSWDNE ENYISLWSIG DFGNLDSDGG
FEGDHQLLCD IRHHGDVMDL QFFDQERIVA ASSTGCVTVF LHHPNNQTLS VNQQWTTAHY
HTGPGSPSYS SAPCTGVVCN NPEIVTVGED GRINLFRADH KEAVRTIDNA DSSTLHAVTF
LRTPEILTVN SIGQLKIWDF RQQGNEPSQI LSLTGDRVPL HCVDRHPNQQ HVVATGGQDG
MLSIWDVRQG TMPVSLLKAH EAEMWEVHFH PSNPEHLFTC SEDGSLWHWD ASTDVPEKSS
LFHQGGRSST FLSHSISNQA NVHQSVISSW LSTDPAKDRI EITSLLPSRS LSVNTLDVLG
PCLVCGTDAE AIYVTRHLFS
//
MIM
608141
*RECORD*
*FIELD* NO
608141
*FIELD* TI
*608141 NUCLEOPORIN, 43-KD; NUP43
;;p42
*FIELD* TX
DESCRIPTION
Bidirectional transport of macromolecules between the cytoplasm and
read morenucleus occurs through nuclear pore complexes (NPCs) embedded in the
nuclear envelope. NPCs are composed of subcomplexes, and NUP43 is part
of one such subcomplex, Nup107-160 (Loiodice et al., 2004).
CLONING
Cronshaw et al. (2002) identified NUP43 as a constituent of a purified
nucleoporin fraction from rat liver nuclei. NUP43 contains WD repeats
and has a calculated molecular mass of about 42 kD. Fluorescence-tagged
NUP43, transfected into HeLa cells, showed punctate nuclear rim
localization typical of nucleoporins.
GENE FUNCTION
By cotransfection and immunoprecipitation of HeLa cells, Loiodice et al.
(2004) showed that NUP43 and several other WD-repeat nucleoporins are
constituents of the Nup107-160 complex. All constituents of this complex
were targeted to kinetochores from prophase to anaphase during mitosis.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the NUP43
gene to chromosome 6 (TMAP stSG53921).
*FIELD* RF
1. Cronshaw, J. M.; Krutchinsky, A. N.; Zhang, W.; Chait, B. T.; Matunis,
M. J.: Proteomic analysis of the mammalian nuclear pore complex. J.
Cell Biol. 158: 915-927, 2002.
2. Loiodice, I.; Alves, A.; Rabut, G.; van Overbeek, M.; Ellenberg,
J.; Sibarita, J.-B.; Doye, V.: The entire Nup107-160 complex, including
three new members, is targeted as one entity to kinetochores in mitosis. Molec.
Biol. Cell 15: 3333-3344, 2004.
*FIELD* CN
Patricia A. Hartz - updated: 3/14/2005
*FIELD* CD
Patricia A. Hartz: 9/30/2003
*FIELD* ED
mgross: 03/16/2005
terry: 3/14/2005
mgross: 9/30/2003
*RECORD*
*FIELD* NO
608141
*FIELD* TI
*608141 NUCLEOPORIN, 43-KD; NUP43
;;p42
*FIELD* TX
DESCRIPTION
Bidirectional transport of macromolecules between the cytoplasm and
read morenucleus occurs through nuclear pore complexes (NPCs) embedded in the
nuclear envelope. NPCs are composed of subcomplexes, and NUP43 is part
of one such subcomplex, Nup107-160 (Loiodice et al., 2004).
CLONING
Cronshaw et al. (2002) identified NUP43 as a constituent of a purified
nucleoporin fraction from rat liver nuclei. NUP43 contains WD repeats
and has a calculated molecular mass of about 42 kD. Fluorescence-tagged
NUP43, transfected into HeLa cells, showed punctate nuclear rim
localization typical of nucleoporins.
GENE FUNCTION
By cotransfection and immunoprecipitation of HeLa cells, Loiodice et al.
(2004) showed that NUP43 and several other WD-repeat nucleoporins are
constituents of the Nup107-160 complex. All constituents of this complex
were targeted to kinetochores from prophase to anaphase during mitosis.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the NUP43
gene to chromosome 6 (TMAP stSG53921).
*FIELD* RF
1. Cronshaw, J. M.; Krutchinsky, A. N.; Zhang, W.; Chait, B. T.; Matunis,
M. J.: Proteomic analysis of the mammalian nuclear pore complex. J.
Cell Biol. 158: 915-927, 2002.
2. Loiodice, I.; Alves, A.; Rabut, G.; van Overbeek, M.; Ellenberg,
J.; Sibarita, J.-B.; Doye, V.: The entire Nup107-160 complex, including
three new members, is targeted as one entity to kinetochores in mitosis. Molec.
Biol. Cell 15: 3333-3344, 2004.
*FIELD* CN
Patricia A. Hartz - updated: 3/14/2005
*FIELD* CD
Patricia A. Hartz: 9/30/2003
*FIELD* ED
mgross: 03/16/2005
terry: 3/14/2005
mgross: 9/30/2003