Full text data of PIP4K2C
PIP4K2C
(PIP5K2C)
[Confidence: low (only semi-automatic identification from reviews)]
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma; 2.7.1.149 (Phosphatidylinositol 5-phosphate 4-kinase type II gamma; PI(5)P 4-kinase type II gamma; PIP4KII-gamma)
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma; 2.7.1.149 (Phosphatidylinositol 5-phosphate 4-kinase type II gamma; PI(5)P 4-kinase type II gamma; PIP4KII-gamma)
UniProt
Q8TBX8
ID PI42C_HUMAN Reviewed; 421 AA.
AC Q8TBX8; B2RDL3; B4DM11; B4DY44; Q9H6N2;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 11-JAN-2011, sequence version 3.
DT 22-JAN-2014, entry version 94.
DE RecName: Full=Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma;
DE EC=2.7.1.149;
DE AltName: Full=Phosphatidylinositol 5-phosphate 4-kinase type II gamma;
DE Short=PI(5)P 4-kinase type II gamma;
DE Short=PIP4KII-gamma;
GN Name=PIP4K2C; Synonyms=PIP5K2C;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Placenta, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M.,
RA Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B.,
RA Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D.,
RA Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z.,
RA Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z.,
RA Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H.,
RA Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H.,
RA Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V.,
RA Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J.,
RA Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A.,
RA Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M.,
RA Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E.,
RA Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M.,
RA Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R.,
RA Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J.,
RA Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C.,
RA Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M.,
RA Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M.,
RA Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P.,
RA Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L.,
RA Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E.,
RA Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C.,
RA Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F.,
RA Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M.,
RA Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S.,
RA Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D.,
RA Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I.,
RA Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T.,
RA Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S.,
RA Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D.,
RA Kucherlapati R., Weinstock G., Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ARG-241.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND SER-349, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of
RT the kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 32-421.
RG Structural genomics consortium (SGC);
RT "Structure of human phosphatidylinositol-4-phosphate 5-kinase, type
RT II, gamma.";
RL Submitted (APR-2007) to the PDB data bank.
CC -!- FUNCTION: May play an important role in the production of
CC Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic
CC reticulum (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 5-
CC phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
CC -!- INTERACTION:
CC Self; NbExp=2; IntAct=EBI-1383637, EBI-1383637;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Membrane. Note=Mostly found in
CC the cytosol and surrounding plasma membrane. However, its presence
CC in the endoplasmic reticulum seems to be a prerequisite for PIP2
CC synthesis (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8TBX8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TBX8-2; Sequence=VSP_042929;
CC Note=No experimental confirmation available;
CC Name=3;
CC IsoId=Q8TBX8-3; Sequence=VSP_043369;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Contains 1 PIPK domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB15223.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
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DR EMBL; AK025708; BAB15223.1; ALT_INIT; mRNA.
DR EMBL; AK297243; BAG59723.1; -; mRNA.
DR EMBL; AK302254; BAG63606.1; -; mRNA.
DR EMBL; AK315588; BAG37960.1; -; mRNA.
DR EMBL; AC022506; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471054; EAW97031.1; -; Genomic_DNA.
DR EMBL; BC028596; AAH28596.1; -; mRNA.
DR RefSeq; NP_001139730.1; NM_001146258.1.
DR RefSeq; NP_001139731.1; NM_001146259.1.
DR RefSeq; NP_001139732.1; NM_001146260.1.
DR RefSeq; NP_079055.3; NM_024779.4.
DR UniGene; Hs.745011; -.
DR PDB; 2GK9; X-ray; 2.80 A; A/B/C/D=32-421.
DR PDBsum; 2GK9; -.
DR ProteinModelPortal; Q8TBX8; -.
DR SMR; Q8TBX8; 39-420.
DR IntAct; Q8TBX8; 1.
DR STRING; 9606.ENSP00000347032; -.
DR BindingDB; Q8TBX8; -.
DR ChEMBL; CHEMBL1770034; -.
DR GuidetoPHARMACOLOGY; 2163; -.
DR PhosphoSite; Q8TBX8; -.
DR DMDM; 146325055; -.
DR PaxDb; Q8TBX8; -.
DR PeptideAtlas; Q8TBX8; -.
DR PRIDE; Q8TBX8; -.
DR DNASU; 79837; -.
DR Ensembl; ENST00000354947; ENSP00000347032; ENSG00000166908.
DR Ensembl; ENST00000422156; ENSP00000412035; ENSG00000166908.
DR Ensembl; ENST00000540759; ENSP00000439878; ENSG00000166908.
DR Ensembl; ENST00000550465; ENSP00000447390; ENSG00000166908.
DR GeneID; 79837; -.
DR KEGG; hsa:79837; -.
DR UCSC; uc001sot.3; human.
DR CTD; 79837; -.
DR GeneCards; GC12P057985; -.
DR H-InvDB; HIX0201836; -.
DR HGNC; HGNC:23786; PIP4K2C.
DR HPA; HPA028658; -.
DR neXtProt; NX_Q8TBX8; -.
DR PharmGKB; PA162399665; -.
DR eggNOG; COG5253; -.
DR HOGENOM; HOG000007832; -.
DR HOVERGEN; HBG000072; -.
DR InParanoid; Q8TBX8; -.
DR KO; K00920; -.
DR OMA; CGLTGPP; -.
DR OrthoDB; EOG708VZZ; -.
DR PhylomeDB; Q8TBX8; -.
DR ChiTaRS; PIP4K2C; human.
DR EvolutionaryTrace; Q8TBX8; -.
DR GenomeRNAi; 79837; -.
DR NextBio; 69498; -.
DR PRO; PR:Q8TBX8; -.
DR ArrayExpress; Q8TBX8; -.
DR Bgee; Q8TBX8; -.
DR CleanEx; HS_PIP4K2C; -.
DR Genevestigator; Q8TBX8; -.
DR GO; GO:0005737; C:cytoplasm; IDA:HPA.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:HPA.
DR GO; GO:0016309; F:1-phosphatidylinositol-5-phosphate 4-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046854; P:phosphatidylinositol phosphorylation; IEA:GOC.
DR Gene3D; 3.30.800.10; -; 1.
DR Gene3D; 3.30.810.10; -; 2.
DR InterPro; IPR023610; PInositol-4-P-5-kinase.
DR InterPro; IPR027483; PInositol-4-P-5-kinase_C.
DR InterPro; IPR002498; PInositol-4-P-5-kinase_core.
DR InterPro; IPR027484; PInositol-4-P-5-kinase_N.
DR InterPro; IPR016034; PInositol-4P-5-kinase_core_sub.
DR PANTHER; PTHR23086; PTHR23086; 1.
DR Pfam; PF01504; PIP5K; 1.
DR SMART; SM00330; PIPKc; 1.
DR PROSITE; PS51455; PIPK; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; ATP-binding;
KW Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding;
KW Phosphoprotein; Polymorphism; Reference proteome; Transferase.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 421 Phosphatidylinositol 5-phosphate 4-kinase
FT type-2 gamma.
FT /FTId=PRO_0000285750.
FT DOMAIN 43 420 PIPK.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 26 26 Phosphoserine.
FT MOD_RES 349 349 Phosphoserine.
FT VAR_SEQ 83 100 Missing (in isoform 3).
FT /FTId=VSP_043369.
FT VAR_SEQ 124 171 Missing (in isoform 2).
FT /FTId=VSP_042929.
FT VARIANT 84 84 V -> A (in dbSNP:rs17550713).
FT /FTId=VAR_032049.
FT VARIANT 241 241 K -> R (in dbSNP:rs17852569).
FT /FTId=VAR_032050.
FT VARIANT 300 300 A -> G (in dbSNP:rs2277319).
FT /FTId=VAR_032051.
FT HELIX 46 62
FT HELIX 73 77
FT STRAND 79 85
FT STRAND 87 89
FT STRAND 92 94
FT STRAND 96 104
FT HELIX 105 113
FT TURN 114 116
FT HELIX 119 127
FT STRAND 147 153
FT HELIX 156 175
FT STRAND 180 182
FT STRAND 185 190
FT STRAND 193 195
FT STRAND 199 204
FT STRAND 209 211
FT HELIX 260 275
FT HELIX 405 415
SQ SEQUENCE 421 AA; 47300 MW; 65D1CBCF0826D476 CRC64;
MASSSVPPAT VSAATAGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV FLWGVAHSIN
ELSQVPPPVM LLPDDFKASS KIKVNNHLFH RENLPSHFKF KEYCPQVFRN LRDRFGIDDQ
DYLVSLTRNP PSESEGSDGR FLISYDRTLV IKEVSSEDIA DMHSNLSNYH QYIVKCHGNT
LLPQFLGMYR VSVDNEDSYM LVMRNMFSHR LPVHRKYDLK GSLVSREASD KEKVKELPTL
KDMDFLNKNQ KVYIGEEEKK IFLEKLKRDV EFLVQLKIMD YSLLLGIHDI IRGSEPEEEA
PVREDESEVD GDCSLTGPPA LVGSYGTSPE GIGGYIHSHR PLGPGEFESF IDVYAIRSAE
GAPQKEVYFM GLIDILTQYD AKKKAAHAAK TVKHGAGAEI STVHPEQYAK RFLDFITNIF
A
//
ID PI42C_HUMAN Reviewed; 421 AA.
AC Q8TBX8; B2RDL3; B4DM11; B4DY44; Q9H6N2;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 11-JAN-2011, sequence version 3.
DT 22-JAN-2014, entry version 94.
DE RecName: Full=Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma;
DE EC=2.7.1.149;
DE AltName: Full=Phosphatidylinositol 5-phosphate 4-kinase type II gamma;
DE Short=PI(5)P 4-kinase type II gamma;
DE Short=PIP4KII-gamma;
GN Name=PIP4K2C; Synonyms=PIP5K2C;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Placenta, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M.,
RA Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B.,
RA Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D.,
RA Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z.,
RA Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z.,
RA Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H.,
RA Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H.,
RA Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V.,
RA Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J.,
RA Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A.,
RA Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M.,
RA Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E.,
RA Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M.,
RA Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R.,
RA Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J.,
RA Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C.,
RA Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M.,
RA Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M.,
RA Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P.,
RA Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L.,
RA Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E.,
RA Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C.,
RA Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F.,
RA Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M.,
RA Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S.,
RA Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D.,
RA Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I.,
RA Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T.,
RA Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S.,
RA Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D.,
RA Kucherlapati R., Weinstock G., Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ARG-241.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND SER-349, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of
RT the kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 32-421.
RG Structural genomics consortium (SGC);
RT "Structure of human phosphatidylinositol-4-phosphate 5-kinase, type
RT II, gamma.";
RL Submitted (APR-2007) to the PDB data bank.
CC -!- FUNCTION: May play an important role in the production of
CC Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic
CC reticulum (By similarity).
CC -!- CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 5-
CC phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
CC -!- INTERACTION:
CC Self; NbExp=2; IntAct=EBI-1383637, EBI-1383637;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Membrane. Note=Mostly found in
CC the cytosol and surrounding plasma membrane. However, its presence
CC in the endoplasmic reticulum seems to be a prerequisite for PIP2
CC synthesis (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8TBX8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TBX8-2; Sequence=VSP_042929;
CC Note=No experimental confirmation available;
CC Name=3;
CC IsoId=Q8TBX8-3; Sequence=VSP_043369;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Contains 1 PIPK domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB15223.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
CC -----------------------------------------------------------------------
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DR EMBL; AK025708; BAB15223.1; ALT_INIT; mRNA.
DR EMBL; AK297243; BAG59723.1; -; mRNA.
DR EMBL; AK302254; BAG63606.1; -; mRNA.
DR EMBL; AK315588; BAG37960.1; -; mRNA.
DR EMBL; AC022506; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471054; EAW97031.1; -; Genomic_DNA.
DR EMBL; BC028596; AAH28596.1; -; mRNA.
DR RefSeq; NP_001139730.1; NM_001146258.1.
DR RefSeq; NP_001139731.1; NM_001146259.1.
DR RefSeq; NP_001139732.1; NM_001146260.1.
DR RefSeq; NP_079055.3; NM_024779.4.
DR UniGene; Hs.745011; -.
DR PDB; 2GK9; X-ray; 2.80 A; A/B/C/D=32-421.
DR PDBsum; 2GK9; -.
DR ProteinModelPortal; Q8TBX8; -.
DR SMR; Q8TBX8; 39-420.
DR IntAct; Q8TBX8; 1.
DR STRING; 9606.ENSP00000347032; -.
DR BindingDB; Q8TBX8; -.
DR ChEMBL; CHEMBL1770034; -.
DR GuidetoPHARMACOLOGY; 2163; -.
DR PhosphoSite; Q8TBX8; -.
DR DMDM; 146325055; -.
DR PaxDb; Q8TBX8; -.
DR PeptideAtlas; Q8TBX8; -.
DR PRIDE; Q8TBX8; -.
DR DNASU; 79837; -.
DR Ensembl; ENST00000354947; ENSP00000347032; ENSG00000166908.
DR Ensembl; ENST00000422156; ENSP00000412035; ENSG00000166908.
DR Ensembl; ENST00000540759; ENSP00000439878; ENSG00000166908.
DR Ensembl; ENST00000550465; ENSP00000447390; ENSG00000166908.
DR GeneID; 79837; -.
DR KEGG; hsa:79837; -.
DR UCSC; uc001sot.3; human.
DR CTD; 79837; -.
DR GeneCards; GC12P057985; -.
DR H-InvDB; HIX0201836; -.
DR HGNC; HGNC:23786; PIP4K2C.
DR HPA; HPA028658; -.
DR neXtProt; NX_Q8TBX8; -.
DR PharmGKB; PA162399665; -.
DR eggNOG; COG5253; -.
DR HOGENOM; HOG000007832; -.
DR HOVERGEN; HBG000072; -.
DR InParanoid; Q8TBX8; -.
DR KO; K00920; -.
DR OMA; CGLTGPP; -.
DR OrthoDB; EOG708VZZ; -.
DR PhylomeDB; Q8TBX8; -.
DR ChiTaRS; PIP4K2C; human.
DR EvolutionaryTrace; Q8TBX8; -.
DR GenomeRNAi; 79837; -.
DR NextBio; 69498; -.
DR PRO; PR:Q8TBX8; -.
DR ArrayExpress; Q8TBX8; -.
DR Bgee; Q8TBX8; -.
DR CleanEx; HS_PIP4K2C; -.
DR Genevestigator; Q8TBX8; -.
DR GO; GO:0005737; C:cytoplasm; IDA:HPA.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:HPA.
DR GO; GO:0016309; F:1-phosphatidylinositol-5-phosphate 4-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046854; P:phosphatidylinositol phosphorylation; IEA:GOC.
DR Gene3D; 3.30.800.10; -; 1.
DR Gene3D; 3.30.810.10; -; 2.
DR InterPro; IPR023610; PInositol-4-P-5-kinase.
DR InterPro; IPR027483; PInositol-4-P-5-kinase_C.
DR InterPro; IPR002498; PInositol-4-P-5-kinase_core.
DR InterPro; IPR027484; PInositol-4-P-5-kinase_N.
DR InterPro; IPR016034; PInositol-4P-5-kinase_core_sub.
DR PANTHER; PTHR23086; PTHR23086; 1.
DR Pfam; PF01504; PIP5K; 1.
DR SMART; SM00330; PIPKc; 1.
DR PROSITE; PS51455; PIPK; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; ATP-binding;
KW Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding;
KW Phosphoprotein; Polymorphism; Reference proteome; Transferase.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 421 Phosphatidylinositol 5-phosphate 4-kinase
FT type-2 gamma.
FT /FTId=PRO_0000285750.
FT DOMAIN 43 420 PIPK.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 26 26 Phosphoserine.
FT MOD_RES 349 349 Phosphoserine.
FT VAR_SEQ 83 100 Missing (in isoform 3).
FT /FTId=VSP_043369.
FT VAR_SEQ 124 171 Missing (in isoform 2).
FT /FTId=VSP_042929.
FT VARIANT 84 84 V -> A (in dbSNP:rs17550713).
FT /FTId=VAR_032049.
FT VARIANT 241 241 K -> R (in dbSNP:rs17852569).
FT /FTId=VAR_032050.
FT VARIANT 300 300 A -> G (in dbSNP:rs2277319).
FT /FTId=VAR_032051.
FT HELIX 46 62
FT HELIX 73 77
FT STRAND 79 85
FT STRAND 87 89
FT STRAND 92 94
FT STRAND 96 104
FT HELIX 105 113
FT TURN 114 116
FT HELIX 119 127
FT STRAND 147 153
FT HELIX 156 175
FT STRAND 180 182
FT STRAND 185 190
FT STRAND 193 195
FT STRAND 199 204
FT STRAND 209 211
FT HELIX 260 275
FT HELIX 405 415
SQ SEQUENCE 421 AA; 47300 MW; 65D1CBCF0826D476 CRC64;
MASSSVPPAT VSAATAGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV FLWGVAHSIN
ELSQVPPPVM LLPDDFKASS KIKVNNHLFH RENLPSHFKF KEYCPQVFRN LRDRFGIDDQ
DYLVSLTRNP PSESEGSDGR FLISYDRTLV IKEVSSEDIA DMHSNLSNYH QYIVKCHGNT
LLPQFLGMYR VSVDNEDSYM LVMRNMFSHR LPVHRKYDLK GSLVSREASD KEKVKELPTL
KDMDFLNKNQ KVYIGEEEKK IFLEKLKRDV EFLVQLKIMD YSLLLGIHDI IRGSEPEEEA
PVREDESEVD GDCSLTGPPA LVGSYGTSPE GIGGYIHSHR PLGPGEFESF IDVYAIRSAE
GAPQKEVYFM GLIDILTQYD AKKKAAHAAK TVKHGAGAEI STVHPEQYAK RFLDFITNIF
A
//