Full text data of GINS3
GINS3
(PSF3)
[Confidence: low (only semi-automatic identification from reviews)]
DNA replication complex GINS protein PSF3 (GINS complex subunit 3)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
DNA replication complex GINS protein PSF3 (GINS complex subunit 3)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q9BRX5
ID PSF3_HUMAN Reviewed; 216 AA.
AC Q9BRX5; B2RDP3; E9PB21; Q9H870;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-2001, sequence version 1.
DT 22-JAN-2014, entry version 96.
DE RecName: Full=DNA replication complex GINS protein PSF3;
DE AltName: Full=GINS complex subunit 3;
GN Name=GINS3; Synonyms=PSF3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X.,
RA Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A.,
RA Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.,
RA Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L.,
RA Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A.,
RA Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D.,
RA Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J.,
RA Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I.,
RA Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W.,
RA Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A.,
RA Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S.,
RA Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L.,
RA Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A.,
RA Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L.,
RA Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N.,
RA Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M.,
RA Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L.,
RA Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D.,
RA Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P.,
RA Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M.,
RA Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Muscle, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUBUNIT, AND INTERACTION WITH DNA PRIMASE.
RX PubMed=17170760; DOI=10.1038/sj.embor.7400870;
RA De Falco M., Ferrari E., De Felice M., Rossi M., Hubscher U.,
RA Pisani F.M.;
RT "The human GINS complex binds to and specifically stimulates human DNA
RT polymerase alpha-primase.";
RL EMBO Rep. 8:99-103(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP STRUCTURE BY ELECTRON MICROSCOPY (33 ANGSTROMS) IN COMPLEX WITH GINS1;
RP GINS2 AND GINS4, SUBUNIT, AND MASS SPECTROMETRY OF GINS COMPLEX.
RX PubMed=17557111; DOI=10.1038/sj.embor.7401002;
RA Boskovic J., Coloma J., Aparicio T., Zhou M., Robinson C.V.,
RA Mendez J., Montoya G.;
RT "Molecular architecture of the human GINS complex.";
RL EMBO Rep. 8:678-684(2007).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH GINS1; GINS2 AND
RP GINS4, AND SUBUNIT.
RX PubMed=17545466; DOI=10.1101/gad.1548107;
RA Choi J.M., Lim H.S., Kim J.J., Song O.K., Cho Y.;
RT "Crystal structure of the human GINS complex.";
RL Genes Dev. 21:1316-1321(2007).
RN [10]
RP FUNCTION, X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH GINS1;
RP GINS2 AND GINS4, AND SUBUNIT.
RX PubMed=17417653; DOI=10.1038/nsmb1231;
RA Kamada K., Kubota Y., Arata T., Shindo Y., Hanaoka F.;
RT "Structure of the human GINS complex and its assembly and functional
RT interface in replication initiation.";
RL Nat. Struct. Mol. Biol. 14:388-396(2007).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.36 ANGSTROMS) OF 2-216 IN COMPLEX WITH GINS1;
RP GINS2 AND GINS4, SUBUNIT, AND REGION.
RX PubMed=17652513; DOI=10.1073/pnas.0705558104;
RA Chang Y.P., Wang G., Bermudez V., Hurwitz J., Chen X.S.;
RT "Crystal structure of the GINS complex and functional insights into
RT its role in DNA replication.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:12685-12690(2007).
CC -!- FUNCTION: The GINS complex plays an essential role in the
CC initiation of DNA replication, and progression of DNA replication
CC forks. GINS complex seems to bind preferentially to single-
CC stranded DNA.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer
CC of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with
CC GINS2. GINS complex interacts with DNA primase in vitro.
CC -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9BRX5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BRX5-2; Sequence=VSP_032737;
CC Name=3;
CC IsoId=Q9BRX5-3; Sequence=VSP_046694;
CC Note=Gene prediction based on EST data;
CC -!- MASS SPECTROMETRY: Mass=98373; Mass_error=13; Method=Electrospray;
CC Range=1-216; Note=This is the measured mass for the GINS complex;
CC Source=PubMed:17557111;
CC -!- SIMILARITY: Belongs to the GINS3/PSF3 family.
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DR EMBL; AK023974; BAB14747.1; -; mRNA.
DR EMBL; AK315622; BAG37990.1; -; mRNA.
DR EMBL; CR457283; CAG33564.1; -; mRNA.
DR EMBL; AC009107; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC009118; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC084063; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471092; EAW82973.1; -; Genomic_DNA.
DR EMBL; CH471092; EAW82976.1; -; Genomic_DNA.
DR EMBL; BC005879; AAH05879.1; -; mRNA.
DR EMBL; BC014437; AAH14437.1; -; mRNA.
DR RefSeq; NP_001119601.1; NM_001126129.1.
DR RefSeq; NP_001119602.1; NM_001126130.1.
DR RefSeq; NP_073607.2; NM_022770.3.
DR UniGene; Hs.47125; -.
DR UniGene; Hs.605546; -.
DR PDB; 2E9X; X-ray; 2.30 A; C/G=1-216.
DR PDB; 2EHO; X-ray; 3.00 A; D/H/L=1-216.
DR PDB; 2Q9Q; X-ray; 2.36 A; D/H=2-216.
DR PDBsum; 2E9X; -.
DR PDBsum; 2EHO; -.
DR PDBsum; 2Q9Q; -.
DR ProteinModelPortal; Q9BRX5; -.
DR SMR; Q9BRX5; 2-193.
DR DIP; DIP-29333N; -.
DR IntAct; Q9BRX5; 1.
DR STRING; 9606.ENSP00000401018; -.
DR PhosphoSite; Q9BRX5; -.
DR DMDM; 74732939; -.
DR PaxDb; Q9BRX5; -.
DR PRIDE; Q9BRX5; -.
DR DNASU; 64785; -.
DR Ensembl; ENST00000318129; ENSP00000318196; ENSG00000181938.
DR Ensembl; ENST00000328514; ENSP00000327449; ENSG00000181938.
DR Ensembl; ENST00000426538; ENSP00000401018; ENSG00000181938.
DR GeneID; 64785; -.
DR KEGG; hsa:64785; -.
DR UCSC; uc010cdj.3; human.
DR CTD; 64785; -.
DR GeneCards; GC16P058426; -.
DR HGNC; HGNC:25851; GINS3.
DR HPA; HPA041216; -.
DR MIM; 610610; gene.
DR neXtProt; NX_Q9BRX5; -.
DR PharmGKB; PA145008327; -.
DR eggNOG; COG5138; -.
DR HOGENOM; HOG000007879; -.
DR HOVERGEN; HBG057574; -.
DR InParanoid; Q9BRX5; -.
DR KO; K10734; -.
DR OMA; QQGWRTV; -.
DR OrthoDB; EOG7HXCS9; -.
DR PhylomeDB; Q9BRX5; -.
DR EvolutionaryTrace; Q9BRX5; -.
DR GenomeRNAi; 64785; -.
DR NextBio; 66832; -.
DR PRO; PR:Q9BRX5; -.
DR ArrayExpress; Q9BRX5; -.
DR Bgee; Q9BRX5; -.
DR CleanEx; HS_GINS3; -.
DR Genevestigator; Q9BRX5; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR021151; GINS_complex.
DR InterPro; IPR010492; GINS_Psf3.
DR PANTHER; PTHR22768; PTHR22768; 1.
DR Pfam; PF05916; Sld5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Complete proteome;
KW DNA replication; Nucleus; Reference proteome.
FT CHAIN 1 216 DNA replication complex GINS protein
FT PSF3.
FT /FTId=PRO_0000327615.
FT REGION 1 16 Not essential for folding and stability
FT of GINS complex, but may regulate
FT accessibility to the central complex
FT pore.
FT VAR_SEQ 62 139 Missing (in isoform 2).
FT /FTId=VSP_032737.
FT VAR_SEQ 63 63 G -> GFALLPRLECSGVIWLTAALTSQAPEILPPQPPMWL
FT VLQG (in isoform 3).
FT /FTId=VSP_046694.
FT STRAND 14 16
FT HELIX 22 27
FT STRAND 30 38
FT STRAND 40 42
FT HELIX 44 46
FT HELIX 55 57
FT STRAND 65 69
FT HELIX 70 76
FT STRAND 79 86
FT HELIX 90 92
FT HELIX 94 102
FT HELIX 104 106
FT TURN 109 111
FT HELIX 116 122
FT HELIX 123 126
FT HELIX 131 153
FT TURN 154 157
FT HELIX 162 165
FT HELIX 170 190
SQ SEQUENCE 216 AA; 24535 MW; A1B264A72371C551 CRC64;
MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV
PQGSKLELPL WLAKGLFDNK RRILSVELPK IYQEGWRTVF SADPNVVDLH KMGPHFYGFG
SQLLHFDSPE NADISQSLLQ TFIGRFRRIM DSSQNAYNED TSALVARLDE MERGLFQTGQ
KGLNDFQCWE KGQASQITAS NLVQNYKKRK FTDMED
//
ID PSF3_HUMAN Reviewed; 216 AA.
AC Q9BRX5; B2RDP3; E9PB21; Q9H870;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-2001, sequence version 1.
DT 22-JAN-2014, entry version 96.
DE RecName: Full=DNA replication complex GINS protein PSF3;
DE AltName: Full=GINS complex subunit 3;
GN Name=GINS3; Synonyms=PSF3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X.,
RA Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A.,
RA Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.,
RA Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L.,
RA Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A.,
RA Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D.,
RA Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J.,
RA Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I.,
RA Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W.,
RA Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A.,
RA Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S.,
RA Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L.,
RA Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A.,
RA Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L.,
RA Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N.,
RA Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M.,
RA Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L.,
RA Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D.,
RA Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P.,
RA Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M.,
RA Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Muscle, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUBUNIT, AND INTERACTION WITH DNA PRIMASE.
RX PubMed=17170760; DOI=10.1038/sj.embor.7400870;
RA De Falco M., Ferrari E., De Felice M., Rossi M., Hubscher U.,
RA Pisani F.M.;
RT "The human GINS complex binds to and specifically stimulates human DNA
RT polymerase alpha-primase.";
RL EMBO Rep. 8:99-103(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP STRUCTURE BY ELECTRON MICROSCOPY (33 ANGSTROMS) IN COMPLEX WITH GINS1;
RP GINS2 AND GINS4, SUBUNIT, AND MASS SPECTROMETRY OF GINS COMPLEX.
RX PubMed=17557111; DOI=10.1038/sj.embor.7401002;
RA Boskovic J., Coloma J., Aparicio T., Zhou M., Robinson C.V.,
RA Mendez J., Montoya G.;
RT "Molecular architecture of the human GINS complex.";
RL EMBO Rep. 8:678-684(2007).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH GINS1; GINS2 AND
RP GINS4, AND SUBUNIT.
RX PubMed=17545466; DOI=10.1101/gad.1548107;
RA Choi J.M., Lim H.S., Kim J.J., Song O.K., Cho Y.;
RT "Crystal structure of the human GINS complex.";
RL Genes Dev. 21:1316-1321(2007).
RN [10]
RP FUNCTION, X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH GINS1;
RP GINS2 AND GINS4, AND SUBUNIT.
RX PubMed=17417653; DOI=10.1038/nsmb1231;
RA Kamada K., Kubota Y., Arata T., Shindo Y., Hanaoka F.;
RT "Structure of the human GINS complex and its assembly and functional
RT interface in replication initiation.";
RL Nat. Struct. Mol. Biol. 14:388-396(2007).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (2.36 ANGSTROMS) OF 2-216 IN COMPLEX WITH GINS1;
RP GINS2 AND GINS4, SUBUNIT, AND REGION.
RX PubMed=17652513; DOI=10.1073/pnas.0705558104;
RA Chang Y.P., Wang G., Bermudez V., Hurwitz J., Chen X.S.;
RT "Crystal structure of the GINS complex and functional insights into
RT its role in DNA replication.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:12685-12690(2007).
CC -!- FUNCTION: The GINS complex plays an essential role in the
CC initiation of DNA replication, and progression of DNA replication
CC forks. GINS complex seems to bind preferentially to single-
CC stranded DNA.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer
CC of GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with
CC GINS2. GINS complex interacts with DNA primase in vitro.
CC -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9BRX5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BRX5-2; Sequence=VSP_032737;
CC Name=3;
CC IsoId=Q9BRX5-3; Sequence=VSP_046694;
CC Note=Gene prediction based on EST data;
CC -!- MASS SPECTROMETRY: Mass=98373; Mass_error=13; Method=Electrospray;
CC Range=1-216; Note=This is the measured mass for the GINS complex;
CC Source=PubMed:17557111;
CC -!- SIMILARITY: Belongs to the GINS3/PSF3 family.
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DR EMBL; AK023974; BAB14747.1; -; mRNA.
DR EMBL; AK315622; BAG37990.1; -; mRNA.
DR EMBL; CR457283; CAG33564.1; -; mRNA.
DR EMBL; AC009107; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC009118; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC084063; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471092; EAW82973.1; -; Genomic_DNA.
DR EMBL; CH471092; EAW82976.1; -; Genomic_DNA.
DR EMBL; BC005879; AAH05879.1; -; mRNA.
DR EMBL; BC014437; AAH14437.1; -; mRNA.
DR RefSeq; NP_001119601.1; NM_001126129.1.
DR RefSeq; NP_001119602.1; NM_001126130.1.
DR RefSeq; NP_073607.2; NM_022770.3.
DR UniGene; Hs.47125; -.
DR UniGene; Hs.605546; -.
DR PDB; 2E9X; X-ray; 2.30 A; C/G=1-216.
DR PDB; 2EHO; X-ray; 3.00 A; D/H/L=1-216.
DR PDB; 2Q9Q; X-ray; 2.36 A; D/H=2-216.
DR PDBsum; 2E9X; -.
DR PDBsum; 2EHO; -.
DR PDBsum; 2Q9Q; -.
DR ProteinModelPortal; Q9BRX5; -.
DR SMR; Q9BRX5; 2-193.
DR DIP; DIP-29333N; -.
DR IntAct; Q9BRX5; 1.
DR STRING; 9606.ENSP00000401018; -.
DR PhosphoSite; Q9BRX5; -.
DR DMDM; 74732939; -.
DR PaxDb; Q9BRX5; -.
DR PRIDE; Q9BRX5; -.
DR DNASU; 64785; -.
DR Ensembl; ENST00000318129; ENSP00000318196; ENSG00000181938.
DR Ensembl; ENST00000328514; ENSP00000327449; ENSG00000181938.
DR Ensembl; ENST00000426538; ENSP00000401018; ENSG00000181938.
DR GeneID; 64785; -.
DR KEGG; hsa:64785; -.
DR UCSC; uc010cdj.3; human.
DR CTD; 64785; -.
DR GeneCards; GC16P058426; -.
DR HGNC; HGNC:25851; GINS3.
DR HPA; HPA041216; -.
DR MIM; 610610; gene.
DR neXtProt; NX_Q9BRX5; -.
DR PharmGKB; PA145008327; -.
DR eggNOG; COG5138; -.
DR HOGENOM; HOG000007879; -.
DR HOVERGEN; HBG057574; -.
DR InParanoid; Q9BRX5; -.
DR KO; K10734; -.
DR OMA; QQGWRTV; -.
DR OrthoDB; EOG7HXCS9; -.
DR PhylomeDB; Q9BRX5; -.
DR EvolutionaryTrace; Q9BRX5; -.
DR GenomeRNAi; 64785; -.
DR NextBio; 66832; -.
DR PRO; PR:Q9BRX5; -.
DR ArrayExpress; Q9BRX5; -.
DR Bgee; Q9BRX5; -.
DR CleanEx; HS_GINS3; -.
DR Genevestigator; Q9BRX5; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR021151; GINS_complex.
DR InterPro; IPR010492; GINS_Psf3.
DR PANTHER; PTHR22768; PTHR22768; 1.
DR Pfam; PF05916; Sld5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Complete proteome;
KW DNA replication; Nucleus; Reference proteome.
FT CHAIN 1 216 DNA replication complex GINS protein
FT PSF3.
FT /FTId=PRO_0000327615.
FT REGION 1 16 Not essential for folding and stability
FT of GINS complex, but may regulate
FT accessibility to the central complex
FT pore.
FT VAR_SEQ 62 139 Missing (in isoform 2).
FT /FTId=VSP_032737.
FT VAR_SEQ 63 63 G -> GFALLPRLECSGVIWLTAALTSQAPEILPPQPPMWL
FT VLQG (in isoform 3).
FT /FTId=VSP_046694.
FT STRAND 14 16
FT HELIX 22 27
FT STRAND 30 38
FT STRAND 40 42
FT HELIX 44 46
FT HELIX 55 57
FT STRAND 65 69
FT HELIX 70 76
FT STRAND 79 86
FT HELIX 90 92
FT HELIX 94 102
FT HELIX 104 106
FT TURN 109 111
FT HELIX 116 122
FT HELIX 123 126
FT HELIX 131 153
FT TURN 154 157
FT HELIX 162 165
FT HELIX 170 190
SQ SEQUENCE 216 AA; 24535 MW; A1B264A72371C551 CRC64;
MSEAYFRVES GALGPEENFL SLDDILMSHE KLPVRTETAM PRLGAFFLER SAGAETDNAV
PQGSKLELPL WLAKGLFDNK RRILSVELPK IYQEGWRTVF SADPNVVDLH KMGPHFYGFG
SQLLHFDSPE NADISQSLLQ TFIGRFRRIM DSSQNAYNED TSALVARLDE MERGLFQTGQ
KGLNDFQCWE KGQASQITAS NLVQNYKKRK FTDMED
//
MIM
610610
*RECORD*
*FIELD* NO
610610
*FIELD* TI
*610610 GINS COMPLEX SUBUNIT 3; GINS3
;;PSF3, S. CEREVISIAE, HOMOLOG OF; PSF3
*FIELD* TX
read more
DESCRIPTION
The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4;
610611), Psf1 (GINS1; 610608), Psf2 (GINS2; 610609), and Psf3. The
formation of the GINS complex is essential for the initiation of DNA
replication in yeast and Xenopus egg extracts (Ueno et al., 2005). See
GINS1 for additional information about the GINS complex.
CLONING
By searching a human database for sequences similar to yeast Psf3,
Takayama et al. (2003) identified GINS3, which they called PSF3. The
deduced protein contains 216 amino acids.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the GINS3
gene to chromosome 16 (TMAP RH65173).
NOMENCLATURE
The GINS complex derives its name from the Japanese words Go, Ichi, Nii,
and San, which mean 5, 1, 2, and 3, respectively, and refer to the
numbering of the yeast homologs of the GINS complex (Takayama et al.,
2003).
*FIELD* RF
1. Takayama, Y.; Kamimura, Y.; Okawa, M.; Muramatsu, S.; Sugino, A.;
Araki, H.: GINS, a novel multiprotein complex required for chromosomal
DNA replication in budding yeast. Genes Dev. 17: 1153-1165, 2003.
2. Ueno, M.; Itoh, M.; Kong, L.; Sugihara, K.; Asano, M.; Takakura,
N.: PSF1 is essential for early embryogenesis in mice. Molec. Cell
Biol. 25: 10528-10532, 2005.
*FIELD* CD
Patricia A. Hartz: 11/29/2006
*FIELD* ED
wwang: 12/04/2006
wwang: 11/29/2006
*RECORD*
*FIELD* NO
610610
*FIELD* TI
*610610 GINS COMPLEX SUBUNIT 3; GINS3
;;PSF3, S. CEREVISIAE, HOMOLOG OF; PSF3
*FIELD* TX
read more
DESCRIPTION
The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4;
610611), Psf1 (GINS1; 610608), Psf2 (GINS2; 610609), and Psf3. The
formation of the GINS complex is essential for the initiation of DNA
replication in yeast and Xenopus egg extracts (Ueno et al., 2005). See
GINS1 for additional information about the GINS complex.
CLONING
By searching a human database for sequences similar to yeast Psf3,
Takayama et al. (2003) identified GINS3, which they called PSF3. The
deduced protein contains 216 amino acids.
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the GINS3
gene to chromosome 16 (TMAP RH65173).
NOMENCLATURE
The GINS complex derives its name from the Japanese words Go, Ichi, Nii,
and San, which mean 5, 1, 2, and 3, respectively, and refer to the
numbering of the yeast homologs of the GINS complex (Takayama et al.,
2003).
*FIELD* RF
1. Takayama, Y.; Kamimura, Y.; Okawa, M.; Muramatsu, S.; Sugino, A.;
Araki, H.: GINS, a novel multiprotein complex required for chromosomal
DNA replication in budding yeast. Genes Dev. 17: 1153-1165, 2003.
2. Ueno, M.; Itoh, M.; Kong, L.; Sugihara, K.; Asano, M.; Takakura,
N.: PSF1 is essential for early embryogenesis in mice. Molec. Cell
Biol. 25: 10528-10532, 2005.
*FIELD* CD
Patricia A. Hartz: 11/29/2006
*FIELD* ED
wwang: 12/04/2006
wwang: 11/29/2006