Full text data of RAB4B
RAB4B
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Ras-related protein Rab-4B
Ras-related protein Rab-4B
UniProt
P61018
ID RAB4B_HUMAN Reviewed; 213 AA.
AC P61018; P22750; Q7Z514; Q9HBR6;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 26-APR-2004, sequence version 1.
DT 22-JAN-2014, entry version 99.
DE RecName: Full=Ras-related protein Rab-4B;
GN Name=RAB4B; ORFNames=PP1596;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Chu J.H., Yu L., Cui W.C., Bi A.D., Huang J., Zhao S.Y.;
RT "Cloning and characterization of a novel human cDNA homologous to
RT R.norvegicus rab4b mRNA.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Hematopoietic stem cell;
RA Huang C., Wu T., Xu S., Gu W., Wang Y., Han Z., Chen Z.;
RT "Novel genes expressed in hematopoietic stem/progenitor cells from
RT myelodysplastic syndrome patients.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H.,
RA Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y.,
RA Shu H., Chen X., Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S.,
RA Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction
RT sequenced by the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 6-183 IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "Crystal structure of human RAB4B in complex with GDP.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Protein transport. Probably involved in vesicular
CC traffic (By similarity).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic
CC side (Potential).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P61018-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P61018-2; Sequence=VSP_013567;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF087861; AAP97171.1; -; mRNA.
DR EMBL; AF165522; AAD45923.1; -; mRNA.
DR EMBL; AF217985; AAG17228.1; -; mRNA.
DR EMBL; AF498935; AAM21083.1; -; mRNA.
DR EMBL; BC046927; AAH46927.1; -; mRNA.
DR RefSeq; NP_057238.3; NM_016154.4.
DR RefSeq; XP_005259061.1; XM_005259004.1.
DR UniGene; Hs.631539; -.
DR UniGene; Hs.730737; -.
DR PDB; 2O52; X-ray; 2.20 A; A/B=6-183.
DR PDBsum; 2O52; -.
DR ProteinModelPortal; P61018; -.
DR SMR; P61018; 6-183.
DR STRING; 9606.ENSP00000349560; -.
DR PhosphoSite; P61018; -.
DR DMDM; 46577635; -.
DR PaxDb; P61018; -.
DR PRIDE; P61018; -.
DR DNASU; 53916; -.
DR Ensembl; ENST00000357052; ENSP00000349560; ENSG00000167578.
DR Ensembl; ENST00000594800; ENSP00000470246; ENSG00000167578.
DR GeneID; 53916; -.
DR KEGG; hsa:53916; -.
DR UCSC; uc002opd.2; human.
DR CTD; 53916; -.
DR GeneCards; GC19P041284; -.
DR HGNC; HGNC:9782; RAB4B.
DR MIM; 612945; gene.
DR neXtProt; NX_P61018; -.
DR PharmGKB; PA34142; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000233968; -.
DR HOVERGEN; HBG009351; -.
DR InParanoid; P61018; -.
DR KO; K07880; -.
DR OMA; EAFLKCS; -.
DR OrthoDB; EOG7QK0CV; -.
DR EvolutionaryTrace; P61018; -.
DR GenomeRNAi; 53916; -.
DR NextBio; 56222; -.
DR PRO; PR:P61018; -.
DR ArrayExpress; P61018; -.
DR Bgee; P61018; -.
DR CleanEx; HS_RAB4B; -.
DR Genevestigator; P61018; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003579; Small_GTPase_Rab_type.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00175; RAB; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Complete proteome;
KW GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding;
KW Prenylation; Protein transport; Reference proteome; Transport.
FT CHAIN 1 213 Ras-related protein Rab-4B.
FT /FTId=PRO_0000121099.
FT NP_BIND 15 23 GTP.
FT NP_BIND 63 67 GTP (By similarity).
FT NP_BIND 121 124 GTP.
FT NP_BIND 151 153 GTP.
FT MOTIF 37 45 Effector region (By similarity).
FT MOD_RES 213 213 Cysteine methyl ester (By similarity).
FT LIPID 211 211 S-geranylgeranyl cysteine (By
FT similarity).
FT LIPID 213 213 S-geranylgeranyl cysteine (By
FT similarity).
FT VAR_SEQ 1 5 MAETY -> MSVSLPLTVMVRERDWIGIHLFSLYLSLPVGI
FT PDFGSIWS (in isoform 2).
FT /FTId=VSP_013567.
FT CONFLICT 4 6 TYD -> DRH (in Ref. 1; AAP97171).
FT STRAND 7 16
FT HELIX 21 29
FT STRAND 45 52
FT STRAND 55 62
FT HELIX 67 70
FT HELIX 75 78
FT STRAND 82 89
FT HELIX 93 97
FT HELIX 99 109
FT STRAND 115 121
FT HELIX 123 128
FT HELIX 133 142
FT STRAND 146 150
FT TURN 152 154
FT HELIX 158 174
FT HELIX 180 182
SQ SEQUENCE 213 AA; 23587 MW; 0C3D76DC3285DB98 CRC64;
MAETYDFLFK FLVIGSAGTG KSCLLHQFIE NKFKQDSNHT IGVEFGSRVV NVGGKTVKLQ
IWDTAGQERF RSVTRSYYRG AAGALLVYDI TSRETYNSLA AWLTDARTLA SPNIVVILCG
NKKDLDPERE VTFLEASRFA QENELMFLET SALTGENVEE AFLKCARTIL NKIDSGELDP
ERMGSGIQYG DASLRQLRQP RSAQAVAPQP CGC
//
ID RAB4B_HUMAN Reviewed; 213 AA.
AC P61018; P22750; Q7Z514; Q9HBR6;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 26-APR-2004, sequence version 1.
DT 22-JAN-2014, entry version 99.
DE RecName: Full=Ras-related protein Rab-4B;
GN Name=RAB4B; ORFNames=PP1596;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Chu J.H., Yu L., Cui W.C., Bi A.D., Huang J., Zhao S.Y.;
RT "Cloning and characterization of a novel human cDNA homologous to
RT R.norvegicus rab4b mRNA.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Hematopoietic stem cell;
RA Huang C., Wu T., Xu S., Gu W., Wang Y., Han Z., Chen Z.;
RT "Novel genes expressed in hematopoietic stem/progenitor cells from
RT myelodysplastic syndrome patients.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H.,
RA Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y.,
RA Shu H., Chen X., Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S.,
RA Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction
RT sequenced by the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 6-183 IN COMPLEX WITH GDP.
RG Structural genomics consortium (SGC);
RT "Crystal structure of human RAB4B in complex with GDP.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Protein transport. Probably involved in vesicular
CC traffic (By similarity).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic
CC side (Potential).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P61018-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P61018-2; Sequence=VSP_013567;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF087861; AAP97171.1; -; mRNA.
DR EMBL; AF165522; AAD45923.1; -; mRNA.
DR EMBL; AF217985; AAG17228.1; -; mRNA.
DR EMBL; AF498935; AAM21083.1; -; mRNA.
DR EMBL; BC046927; AAH46927.1; -; mRNA.
DR RefSeq; NP_057238.3; NM_016154.4.
DR RefSeq; XP_005259061.1; XM_005259004.1.
DR UniGene; Hs.631539; -.
DR UniGene; Hs.730737; -.
DR PDB; 2O52; X-ray; 2.20 A; A/B=6-183.
DR PDBsum; 2O52; -.
DR ProteinModelPortal; P61018; -.
DR SMR; P61018; 6-183.
DR STRING; 9606.ENSP00000349560; -.
DR PhosphoSite; P61018; -.
DR DMDM; 46577635; -.
DR PaxDb; P61018; -.
DR PRIDE; P61018; -.
DR DNASU; 53916; -.
DR Ensembl; ENST00000357052; ENSP00000349560; ENSG00000167578.
DR Ensembl; ENST00000594800; ENSP00000470246; ENSG00000167578.
DR GeneID; 53916; -.
DR KEGG; hsa:53916; -.
DR UCSC; uc002opd.2; human.
DR CTD; 53916; -.
DR GeneCards; GC19P041284; -.
DR HGNC; HGNC:9782; RAB4B.
DR MIM; 612945; gene.
DR neXtProt; NX_P61018; -.
DR PharmGKB; PA34142; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000233968; -.
DR HOVERGEN; HBG009351; -.
DR InParanoid; P61018; -.
DR KO; K07880; -.
DR OMA; EAFLKCS; -.
DR OrthoDB; EOG7QK0CV; -.
DR EvolutionaryTrace; P61018; -.
DR GenomeRNAi; 53916; -.
DR NextBio; 56222; -.
DR PRO; PR:P61018; -.
DR ArrayExpress; P61018; -.
DR Bgee; P61018; -.
DR CleanEx; HS_RAB4B; -.
DR Genevestigator; P61018; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003579; Small_GTPase_Rab_type.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00175; RAB; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Complete proteome;
KW GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding;
KW Prenylation; Protein transport; Reference proteome; Transport.
FT CHAIN 1 213 Ras-related protein Rab-4B.
FT /FTId=PRO_0000121099.
FT NP_BIND 15 23 GTP.
FT NP_BIND 63 67 GTP (By similarity).
FT NP_BIND 121 124 GTP.
FT NP_BIND 151 153 GTP.
FT MOTIF 37 45 Effector region (By similarity).
FT MOD_RES 213 213 Cysteine methyl ester (By similarity).
FT LIPID 211 211 S-geranylgeranyl cysteine (By
FT similarity).
FT LIPID 213 213 S-geranylgeranyl cysteine (By
FT similarity).
FT VAR_SEQ 1 5 MAETY -> MSVSLPLTVMVRERDWIGIHLFSLYLSLPVGI
FT PDFGSIWS (in isoform 2).
FT /FTId=VSP_013567.
FT CONFLICT 4 6 TYD -> DRH (in Ref. 1; AAP97171).
FT STRAND 7 16
FT HELIX 21 29
FT STRAND 45 52
FT STRAND 55 62
FT HELIX 67 70
FT HELIX 75 78
FT STRAND 82 89
FT HELIX 93 97
FT HELIX 99 109
FT STRAND 115 121
FT HELIX 123 128
FT HELIX 133 142
FT STRAND 146 150
FT TURN 152 154
FT HELIX 158 174
FT HELIX 180 182
SQ SEQUENCE 213 AA; 23587 MW; 0C3D76DC3285DB98 CRC64;
MAETYDFLFK FLVIGSAGTG KSCLLHQFIE NKFKQDSNHT IGVEFGSRVV NVGGKTVKLQ
IWDTAGQERF RSVTRSYYRG AAGALLVYDI TSRETYNSLA AWLTDARTLA SPNIVVILCG
NKKDLDPERE VTFLEASRFA QENELMFLET SALTGENVEE AFLKCARTIL NKIDSGELDP
ERMGSGIQYG DASLRQLRQP RSAQAVAPQP CGC
//
MIM
612945
*RECORD*
*FIELD* NO
612945
*FIELD* TI
*612945 RAS-ASSOCIATED PROTEIN RAB4B; RAB4B
*FIELD* TX
DESCRIPTION
RAB proteins, such as RAB4B, are members of the RAS superfamily of small
read moreGTPases that are involved in vesicular trafficking (He et al., 2002).
CLONING
By searching databases for RAS family members, followed by PCR, He et
al. (2002) cloned RAB4B. The deduced 212-amino acid RAB4B protein
contains 4 highly conserved GTPase motifs and a C-terminal
geranylgeranylation motif. Northern blot analysis detected a major
transcript of about 1.2 kb that showed highest expressed in ovary,
followed by thymus and prostate. Lower expression was detected in
spleen, testis, small intestine, colon, peripheral blood, lung,
pancreas, heart, and kidney. Little to no expression was detected in
brain, placenta, liver, and skeletal muscle.
GENE STRUCTURE
He et al. (2002) determined that the RAB4B gene contains 8 exons and
spans over 20 kb.
MAPPING
By radiation hybrid analysis, He et al. (2002) mapped the RAB4B gene to
chromosome 19q13.2-q13.3.
Barbosa et al. (1995) mapped the mouse Rab4b gene to proximal chromosome
7.
*FIELD* RF
1. Barbosa, M. D. F. S.; Johnson, S. A.; Achey, K.; Gutierrez, M.;
Wakeland, E. K.; Zerial, M.; Kingsmore, S. F.: The Rab protein family:
genetic mapping of six Rab genes in the mouse. Genomics 30: 439-444,
1995.
2. He, H.; Dai, F.; Yu, L.; She, X.; Zhao, Y.; Jiang, J.; Chen, X.;
Zhao, S.: Identification and characterization of nine novel human
small GTPases showing variable expressions in liver cancer tissues. Gene
Expr. 10: 231-242, 2002.
*FIELD* CD
Patricia A. Hartz: 7/30/2009
*FIELD* ED
mgross: 07/30/2009
*RECORD*
*FIELD* NO
612945
*FIELD* TI
*612945 RAS-ASSOCIATED PROTEIN RAB4B; RAB4B
*FIELD* TX
DESCRIPTION
RAB proteins, such as RAB4B, are members of the RAS superfamily of small
read moreGTPases that are involved in vesicular trafficking (He et al., 2002).
CLONING
By searching databases for RAS family members, followed by PCR, He et
al. (2002) cloned RAB4B. The deduced 212-amino acid RAB4B protein
contains 4 highly conserved GTPase motifs and a C-terminal
geranylgeranylation motif. Northern blot analysis detected a major
transcript of about 1.2 kb that showed highest expressed in ovary,
followed by thymus and prostate. Lower expression was detected in
spleen, testis, small intestine, colon, peripheral blood, lung,
pancreas, heart, and kidney. Little to no expression was detected in
brain, placenta, liver, and skeletal muscle.
GENE STRUCTURE
He et al. (2002) determined that the RAB4B gene contains 8 exons and
spans over 20 kb.
MAPPING
By radiation hybrid analysis, He et al. (2002) mapped the RAB4B gene to
chromosome 19q13.2-q13.3.
Barbosa et al. (1995) mapped the mouse Rab4b gene to proximal chromosome
7.
*FIELD* RF
1. Barbosa, M. D. F. S.; Johnson, S. A.; Achey, K.; Gutierrez, M.;
Wakeland, E. K.; Zerial, M.; Kingsmore, S. F.: The Rab protein family:
genetic mapping of six Rab genes in the mouse. Genomics 30: 439-444,
1995.
2. He, H.; Dai, F.; Yu, L.; She, X.; Zhao, Y.; Jiang, J.; Chen, X.;
Zhao, S.: Identification and characterization of nine novel human
small GTPases showing variable expressions in liver cancer tissues. Gene
Expr. 10: 231-242, 2002.
*FIELD* CD
Patricia A. Hartz: 7/30/2009
*FIELD* ED
mgross: 07/30/2009