Full text data of RAB6C
RAB6C
(WTH3)
[Confidence: low (only semi-automatic identification from reviews)]
Ras-related protein Rab-6C (Rab6-like protein WTH3)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Ras-related protein Rab-6C (Rab6-like protein WTH3)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q9H0N0
ID RAB6C_HUMAN Reviewed; 254 AA.
AC Q9H0N0; Q53RU3; Q6FIF7; Q9P128;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
read moreDT 15-JAN-2008, sequence version 2.
DT 22-JAN-2014, entry version 105.
DE RecName: Full=Ras-related protein Rab-6C;
DE AltName: Full=Rab6-like protein WTH3;
GN Name=RAB6C; Synonyms=WTH3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT THR-159.
RX PubMed=12007787; DOI=10.1016/S0167-4889(02)00164-7;
RA Shan J., Yuan L., Budman D.R., Xu H.-P.;
RT "WTH3, a new member of the Rab6 gene family, and multidrug
RT resistance.";
RL Biochim. Biophys. Acta 1589:112-123(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-159.
RC TISSUE=Kidney;
RX PubMed=11230166; DOI=10.1101/gr.GR1547R;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H.,
RA Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N.,
RA Mewes H.-W., Ottenwaelder B., Obermaier B., Tampe J., Heubner D.,
RA Wambutt R., Korn B., Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and
RT analysis of 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-159.
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-159.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-175, AND VARIANT THR-159.
RX PubMed=11054569; DOI=10.1016/S0378-1119(00)00395-4;
RA Shan J., Mason J.M., Yuan L., Barcia M., Porti D., Calabro A.,
RA Budman D., Vinciguerra V., Xu H.-P.;
RT "Rab6c, a new member of the Rab gene family, is involved in drug
RT resistance in MCF7/AdrR cells.";
RL Gene 257:67-75(2000).
RN [8]
RP GTP-BINDING, AND SUBCELLULAR LOCATION.
RX PubMed=16103095; DOI=10.1158/0008-5472.CAN-05-0658;
RA Tian K., Jurukovski V., Yuan L., Shan J., Xu H.;
RT "WTH3, which encodes a small G protein, is differentially regulated in
RT multidrug-resistant and sensitive MCF7 cells.";
RL Cancer Res. 65:7421-7428(2005).
RN [9]
RP FUNCTION.
RX PubMed=17426708; DOI=10.1038/sj.bjc.6603724;
RA Tian K., Wang Y., Xu H.;
RT "WTH3 is a direct target of the p53 protein.";
RL Br. J. Cancer 96:1579-1586(2007).
RN [10]
RP FUNCTION.
RX PubMed=18992151; DOI=10.1186/1471-2407-8-327;
RA Tian K., Wang Y., Huang Y., Sun B., Li Y., Xu H.;
RT "Methylation of WTH3, a possible drug resistant gene, inhibits p53
RT regulated expression.";
RL BMC Cancer 8:327-327(2008).
RN [11]
RP RETROGENE, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF ALA-25 AND ILE-45.
RX PubMed=20064528; DOI=10.1016/j.jmb.2010.01.009;
RA Young J., Menetrey J., Goud B.;
RT "RAB6C is a retrogene that encodes a centrosomal protein involved in
RT cell cycle progression.";
RL J. Mol. Biol. 397:69-88(2010).
RN [12]
RP AMPYLATION AT TYR-82.
RX PubMed=21822290; DOI=10.1038/nature10335;
RA Mukherjee S., Liu X., Arasaki K., McDonough J., Galan J.E., Roy C.R.;
RT "Modulation of Rab GTPase function by a protein phosphocholine
RT transferase.";
RL Nature 477:103-106(2011).
CC -!- FUNCTION: May be involved in the regulation of centrosome
CC duplication and cell cycle progression.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome.
CC -!- TISSUE SPECIFICITY: Highest levels are found in fetal and adult
CC brain, prostate, testis and spinal cord. Undetectable expression
CC in adrenal gland, skeletal muscle, bone marrow, fetal, and adult
CC liver, heart, salivary gland, and trachea. Detected in the HEK293,
CC HEK293T, LNCaP, MCF-7, T-47D and EVSA-T cell lines (at protein
CC level).
CC -!- MISCELLANEOUS: Primate-specific retrogene derived from isoform 2
CC of RAB6A transcript.
CC -!- MISCELLANEOUS: Previously reported to exhibit GTP-binding affinity
CC comparable to that of RAB6A (PubMed:16103095). In contrast
CC (PubMed:20064528) concludes that RAB6C is an inefficient GTP-
CC binding.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF309646; AAN39685.1; -; mRNA.
DR EMBL; AL136727; CAB66661.1; -; mRNA.
DR EMBL; CR533469; CAG38500.1; -; mRNA.
DR EMBL; AC079776; AAY15045.1; -; Genomic_DNA.
DR EMBL; CH471103; EAW95362.1; -; Genomic_DNA.
DR EMBL; CH471058; EAX11700.1; -; Genomic_DNA.
DR EMBL; BC120999; AAI21000.1; -; mRNA.
DR EMBL; AF124200; AAF28422.1; -; Genomic_DNA.
DR RefSeq; NP_115520.2; NM_032144.2.
DR UniGene; Hs.591552; -.
DR ProteinModelPortal; Q9H0N0; -.
DR SMR; Q9H0N0; 14-177.
DR IntAct; Q9H0N0; 2.
DR MINT; MINT-2817186; -.
DR STRING; 9606.ENSP00000387307; -.
DR PhosphoSite; Q9H0N0; -.
DR DMDM; 166214970; -.
DR PaxDb; Q9H0N0; -.
DR PRIDE; Q9H0N0; -.
DR DNASU; 84084; -.
DR Ensembl; ENST00000410061; ENSP00000387307; ENSG00000222014.
DR GeneID; 84084; -.
DR KEGG; hsa:84084; -.
DR UCSC; uc002tpx.1; human.
DR CTD; 84084; -.
DR GeneCards; GC02P130737; -.
DR H-InvDB; HIX0002449; -.
DR H-InvDB; HIX0023949; -.
DR HGNC; HGNC:16525; RAB6C.
DR MIM; 612909; gene.
DR neXtProt; NX_Q9H0N0; -.
DR PharmGKB; PA34148; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000233968; -.
DR HOVERGEN; HBG009351; -.
DR InParanoid; Q9H0N0; -.
DR KO; K07895; -.
DR OMA; RYIRDSA; -.
DR OrthoDB; EOG77DJ7M; -.
DR GeneWiki; RAB6C; -.
DR GenomeRNAi; 84084; -.
DR NextBio; 73315; -.
DR PRO; PR:Q9H0N0; -.
DR Bgee; Q9H0N0; -.
DR Genevestigator; Q9H0N0; -.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IDA:UniProtKB.
DR GO; GO:0022402; P:cell cycle process; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR GO; GO:0010824; P:regulation of centrosome duplication; IMP:UniProtKB.
DR GO; GO:0042493; P:response to drug; IDA:UniProtKB.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; TAS:UniProtKB.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003579; Small_GTPase_Rab_type.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00175; RAB; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding;
KW Nucleotide-binding; Nucleus; Polymorphism; Reference proteome.
FT CHAIN 1 254 Ras-related protein Rab-6C.
FT /FTId=PRO_0000121117.
FT NP_BIND 20 27 GTP (By similarity).
FT NP_BIND 68 72 GTP (By similarity).
FT NP_BIND 126 129 GTP (By similarity).
FT REGION 1 174 Required for centrosome localization.
FT MOTIF 42 50 Effector region (By similarity).
FT MOD_RES 82 82 O-AMP-tyrosine; by Legionella DrrA.
FT VARIANT 159 159 A -> T.
FT /FTId=VAR_038161.
FT MUTAGEN 25 25 A->G: No GTP binding. No GTP binding;
FT when associated with T-45.
FT MUTAGEN 45 45 I->T: No GTP binding; when associated
FT with G-25.
SQ SEQUENCE 254 AA; 28355 MW; 72A4D8DED771F754 CRC64;
MSAGGDFGNP LRKFKLVFLG EQSVAKTSLI TRFRYDSFDN TYQAIIGIDF LSKTMYLEDG
TIGLRLWDTA GQERLRSLIP RYIRDSAAAV VVYDITNVNS FQQTTKWIDD VRTERGSDVI
ITLVGNRTDL ADKRQVSVEE GERKAKGLNV TFIETRAKAG YNVKQLFRRV AAALPGMEST
QDGSREDMSD IKLEKPQEQT VSEGGCSCYS PMSSSTLPQK PPYSFIDCSV NIGLNLFPSL
ITFCNSSLLP VSWR
//
ID RAB6C_HUMAN Reviewed; 254 AA.
AC Q9H0N0; Q53RU3; Q6FIF7; Q9P128;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
read moreDT 15-JAN-2008, sequence version 2.
DT 22-JAN-2014, entry version 105.
DE RecName: Full=Ras-related protein Rab-6C;
DE AltName: Full=Rab6-like protein WTH3;
GN Name=RAB6C; Synonyms=WTH3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT THR-159.
RX PubMed=12007787; DOI=10.1016/S0167-4889(02)00164-7;
RA Shan J., Yuan L., Budman D.R., Xu H.-P.;
RT "WTH3, a new member of the Rab6 gene family, and multidrug
RT resistance.";
RL Biochim. Biophys. Acta 1589:112-123(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-159.
RC TISSUE=Kidney;
RX PubMed=11230166; DOI=10.1101/gr.GR1547R;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H.,
RA Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N.,
RA Mewes H.-W., Ottenwaelder B., Obermaier B., Tampe J., Heubner D.,
RA Wambutt R., Korn B., Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and
RT analysis of 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-159.
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-159.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-175, AND VARIANT THR-159.
RX PubMed=11054569; DOI=10.1016/S0378-1119(00)00395-4;
RA Shan J., Mason J.M., Yuan L., Barcia M., Porti D., Calabro A.,
RA Budman D., Vinciguerra V., Xu H.-P.;
RT "Rab6c, a new member of the Rab gene family, is involved in drug
RT resistance in MCF7/AdrR cells.";
RL Gene 257:67-75(2000).
RN [8]
RP GTP-BINDING, AND SUBCELLULAR LOCATION.
RX PubMed=16103095; DOI=10.1158/0008-5472.CAN-05-0658;
RA Tian K., Jurukovski V., Yuan L., Shan J., Xu H.;
RT "WTH3, which encodes a small G protein, is differentially regulated in
RT multidrug-resistant and sensitive MCF7 cells.";
RL Cancer Res. 65:7421-7428(2005).
RN [9]
RP FUNCTION.
RX PubMed=17426708; DOI=10.1038/sj.bjc.6603724;
RA Tian K., Wang Y., Xu H.;
RT "WTH3 is a direct target of the p53 protein.";
RL Br. J. Cancer 96:1579-1586(2007).
RN [10]
RP FUNCTION.
RX PubMed=18992151; DOI=10.1186/1471-2407-8-327;
RA Tian K., Wang Y., Huang Y., Sun B., Li Y., Xu H.;
RT "Methylation of WTH3, a possible drug resistant gene, inhibits p53
RT regulated expression.";
RL BMC Cancer 8:327-327(2008).
RN [11]
RP RETROGENE, FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF ALA-25 AND ILE-45.
RX PubMed=20064528; DOI=10.1016/j.jmb.2010.01.009;
RA Young J., Menetrey J., Goud B.;
RT "RAB6C is a retrogene that encodes a centrosomal protein involved in
RT cell cycle progression.";
RL J. Mol. Biol. 397:69-88(2010).
RN [12]
RP AMPYLATION AT TYR-82.
RX PubMed=21822290; DOI=10.1038/nature10335;
RA Mukherjee S., Liu X., Arasaki K., McDonough J., Galan J.E., Roy C.R.;
RT "Modulation of Rab GTPase function by a protein phosphocholine
RT transferase.";
RL Nature 477:103-106(2011).
CC -!- FUNCTION: May be involved in the regulation of centrosome
CC duplication and cell cycle progression.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton,
CC microtubule organizing center, centrosome.
CC -!- TISSUE SPECIFICITY: Highest levels are found in fetal and adult
CC brain, prostate, testis and spinal cord. Undetectable expression
CC in adrenal gland, skeletal muscle, bone marrow, fetal, and adult
CC liver, heart, salivary gland, and trachea. Detected in the HEK293,
CC HEK293T, LNCaP, MCF-7, T-47D and EVSA-T cell lines (at protein
CC level).
CC -!- MISCELLANEOUS: Primate-specific retrogene derived from isoform 2
CC of RAB6A transcript.
CC -!- MISCELLANEOUS: Previously reported to exhibit GTP-binding affinity
CC comparable to that of RAB6A (PubMed:16103095). In contrast
CC (PubMed:20064528) concludes that RAB6C is an inefficient GTP-
CC binding.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF309646; AAN39685.1; -; mRNA.
DR EMBL; AL136727; CAB66661.1; -; mRNA.
DR EMBL; CR533469; CAG38500.1; -; mRNA.
DR EMBL; AC079776; AAY15045.1; -; Genomic_DNA.
DR EMBL; CH471103; EAW95362.1; -; Genomic_DNA.
DR EMBL; CH471058; EAX11700.1; -; Genomic_DNA.
DR EMBL; BC120999; AAI21000.1; -; mRNA.
DR EMBL; AF124200; AAF28422.1; -; Genomic_DNA.
DR RefSeq; NP_115520.2; NM_032144.2.
DR UniGene; Hs.591552; -.
DR ProteinModelPortal; Q9H0N0; -.
DR SMR; Q9H0N0; 14-177.
DR IntAct; Q9H0N0; 2.
DR MINT; MINT-2817186; -.
DR STRING; 9606.ENSP00000387307; -.
DR PhosphoSite; Q9H0N0; -.
DR DMDM; 166214970; -.
DR PaxDb; Q9H0N0; -.
DR PRIDE; Q9H0N0; -.
DR DNASU; 84084; -.
DR Ensembl; ENST00000410061; ENSP00000387307; ENSG00000222014.
DR GeneID; 84084; -.
DR KEGG; hsa:84084; -.
DR UCSC; uc002tpx.1; human.
DR CTD; 84084; -.
DR GeneCards; GC02P130737; -.
DR H-InvDB; HIX0002449; -.
DR H-InvDB; HIX0023949; -.
DR HGNC; HGNC:16525; RAB6C.
DR MIM; 612909; gene.
DR neXtProt; NX_Q9H0N0; -.
DR PharmGKB; PA34148; -.
DR eggNOG; COG1100; -.
DR HOGENOM; HOG000233968; -.
DR HOVERGEN; HBG009351; -.
DR InParanoid; Q9H0N0; -.
DR KO; K07895; -.
DR OMA; RYIRDSA; -.
DR OrthoDB; EOG77DJ7M; -.
DR GeneWiki; RAB6C; -.
DR GenomeRNAi; 84084; -.
DR NextBio; 73315; -.
DR PRO; PR:Q9H0N0; -.
DR Bgee; Q9H0N0; -.
DR Genevestigator; Q9H0N0; -.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IDA:UniProtKB.
DR GO; GO:0022402; P:cell cycle process; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:InterPro.
DR GO; GO:0010824; P:regulation of centrosome duplication; IMP:UniProtKB.
DR GO; GO:0042493; P:response to drug; IDA:UniProtKB.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; TAS:UniProtKB.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR InterPro; IPR003579; Small_GTPase_Rab_type.
DR Pfam; PF00071; Ras; 1.
DR PRINTS; PR00449; RASTRNSFRMNG.
DR SMART; SM00175; RAB; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51419; RAB; 1.
PE 1: Evidence at protein level;
KW Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding;
KW Nucleotide-binding; Nucleus; Polymorphism; Reference proteome.
FT CHAIN 1 254 Ras-related protein Rab-6C.
FT /FTId=PRO_0000121117.
FT NP_BIND 20 27 GTP (By similarity).
FT NP_BIND 68 72 GTP (By similarity).
FT NP_BIND 126 129 GTP (By similarity).
FT REGION 1 174 Required for centrosome localization.
FT MOTIF 42 50 Effector region (By similarity).
FT MOD_RES 82 82 O-AMP-tyrosine; by Legionella DrrA.
FT VARIANT 159 159 A -> T.
FT /FTId=VAR_038161.
FT MUTAGEN 25 25 A->G: No GTP binding. No GTP binding;
FT when associated with T-45.
FT MUTAGEN 45 45 I->T: No GTP binding; when associated
FT with G-25.
SQ SEQUENCE 254 AA; 28355 MW; 72A4D8DED771F754 CRC64;
MSAGGDFGNP LRKFKLVFLG EQSVAKTSLI TRFRYDSFDN TYQAIIGIDF LSKTMYLEDG
TIGLRLWDTA GQERLRSLIP RYIRDSAAAV VVYDITNVNS FQQTTKWIDD VRTERGSDVI
ITLVGNRTDL ADKRQVSVEE GERKAKGLNV TFIETRAKAG YNVKQLFRRV AAALPGMEST
QDGSREDMSD IKLEKPQEQT VSEGGCSCYS PMSSSTLPQK PPYSFIDCSV NIGLNLFPSL
ITFCNSSLLP VSWR
//
MIM
612909
*RECORD*
*FIELD* NO
612909
*FIELD* TI
*612909 RAS-ASSOCIATED PROTEIN RAB6C; RAB6C
;;WTH3
*FIELD* TX
CLONING
By methylation-sensitive representational difference analysis, Shan et
read moreal. (2002) isolated a DNA fragment of RAB6C that was hypermethylated in
a multidrug-resistant MCF7 breast cancer cell line. Using 3-prime RACE
of a combined human tissue cDNA library, they obtained full-length
RAB6C, which they called WTH3. The deduced 254-amino acid WTH3 protein
shares significant homology with 2 isoforms of RAB6A (179513), but it
has a C-terminal extension compared with these proteins.
GENE FUNCTION
Using quantitative RT-PCR, Shan et al. (2002) found that expression of
WTH3 was reduced in multidrug-resistant cancer cell lines compared with
the parental cell lines. Reexpression of WTH3 in multidrug-resistant
cell lines increased their sensitivity to several anticancer drugs,
including doxorubicin. Flow cytometry and fluorescence microscopy
suggested that WTH3 stimulated cellular uptake and retention of
doxorubicin.
Tian et al. (2005) found that the WTH3 promoter region was
hypermethylated in multidrug-resistant MCF7 cells compared with the
parental cell line. EMSA showed that different pools of nuclear proteins
were attracted to the hypermethylated and nonmethylated WTH3 probes.
Tian et al. (2005) concluded that expression of WTH3 is controlled by
both epigenetic modification and transcription factor modulation.
Tian et al. (2007) identified a p53 (TP53; 191170)-binding site in the
promoter region of the WTH3 gene and showed that WTH3 was upregulated by
p53 in vitro and in vivo. Overexpression of WTH3 promoted an apoptotic
phenotype in human cells. Tian et al. (2007) concluded that the
antiapoptotic effect of WTH3 is linked to p53 expression.
GENE STRUCTURE
Tian et al. (2005) determined that the RAB6C promoter region contains 2
atypical TATA boxes and 2 atypical CAATT boxes. They identified a
GC-rich region that includes a CpG island and extends from position -485
relative to the translation initiation site into the 5-prime coding
region. The promoter region contains several putative transcription
factor-binding sites.
MAPPING
Hartz (2009) mapped the RAB6C gene to chromosome 2q21.1 based on an
alignment of the RAB6C sequence (GenBank GENBANK AL136727) with the
genomic sequence (build 36.1).
*FIELD* SA
Shan et al. (2000)
*FIELD* RF
1. Hartz, P. A.: Personal Communication. Baltimore, Md. 7/10/2009.
2. Shan, J.; Mason, J. M.; Yuan, L.; Barcia, M.; Porti, D.; Calabro,
A.; Budman, D.; Vinciguerra, V.; Xu, H.: Rab6c, a new member of the
Rab gene family, is involved in drug resistance in MCF7/AdrR cells. Gene 257:
67-75, 2000.
3. Shan, J.; Yuan, L.; Budman, D. R.; Xu, H.: WTH3, a new member
of the Rab6 gene family, and multidrug resistance. Biochim. Biophys.
Acta 1589: 112-123, 2002.
4. Tian, K.; Jurukovski, V.; Yuan, L.; Shan, J.; Xu, H.: WTH3, which
encodes a small G protein, is differentially regulated in multidrug-resistant
and sensitive MCF7 cells. Cancer Res. 65: 7421-7428, 2005.
5. Tian, K.; Wang, Y.; Xu, H.: WTH3 is a direct target of the p53
protein. Brit. J. Cancer 96: 1579-1586, 2007.
*FIELD* CD
Patricia A. Hartz: 7/13/2009
*FIELD* ED
mgross: 07/13/2009
*RECORD*
*FIELD* NO
612909
*FIELD* TI
*612909 RAS-ASSOCIATED PROTEIN RAB6C; RAB6C
;;WTH3
*FIELD* TX
CLONING
By methylation-sensitive representational difference analysis, Shan et
read moreal. (2002) isolated a DNA fragment of RAB6C that was hypermethylated in
a multidrug-resistant MCF7 breast cancer cell line. Using 3-prime RACE
of a combined human tissue cDNA library, they obtained full-length
RAB6C, which they called WTH3. The deduced 254-amino acid WTH3 protein
shares significant homology with 2 isoforms of RAB6A (179513), but it
has a C-terminal extension compared with these proteins.
GENE FUNCTION
Using quantitative RT-PCR, Shan et al. (2002) found that expression of
WTH3 was reduced in multidrug-resistant cancer cell lines compared with
the parental cell lines. Reexpression of WTH3 in multidrug-resistant
cell lines increased their sensitivity to several anticancer drugs,
including doxorubicin. Flow cytometry and fluorescence microscopy
suggested that WTH3 stimulated cellular uptake and retention of
doxorubicin.
Tian et al. (2005) found that the WTH3 promoter region was
hypermethylated in multidrug-resistant MCF7 cells compared with the
parental cell line. EMSA showed that different pools of nuclear proteins
were attracted to the hypermethylated and nonmethylated WTH3 probes.
Tian et al. (2005) concluded that expression of WTH3 is controlled by
both epigenetic modification and transcription factor modulation.
Tian et al. (2007) identified a p53 (TP53; 191170)-binding site in the
promoter region of the WTH3 gene and showed that WTH3 was upregulated by
p53 in vitro and in vivo. Overexpression of WTH3 promoted an apoptotic
phenotype in human cells. Tian et al. (2007) concluded that the
antiapoptotic effect of WTH3 is linked to p53 expression.
GENE STRUCTURE
Tian et al. (2005) determined that the RAB6C promoter region contains 2
atypical TATA boxes and 2 atypical CAATT boxes. They identified a
GC-rich region that includes a CpG island and extends from position -485
relative to the translation initiation site into the 5-prime coding
region. The promoter region contains several putative transcription
factor-binding sites.
MAPPING
Hartz (2009) mapped the RAB6C gene to chromosome 2q21.1 based on an
alignment of the RAB6C sequence (GenBank GENBANK AL136727) with the
genomic sequence (build 36.1).
*FIELD* SA
Shan et al. (2000)
*FIELD* RF
1. Hartz, P. A.: Personal Communication. Baltimore, Md. 7/10/2009.
2. Shan, J.; Mason, J. M.; Yuan, L.; Barcia, M.; Porti, D.; Calabro,
A.; Budman, D.; Vinciguerra, V.; Xu, H.: Rab6c, a new member of the
Rab gene family, is involved in drug resistance in MCF7/AdrR cells. Gene 257:
67-75, 2000.
3. Shan, J.; Yuan, L.; Budman, D. R.; Xu, H.: WTH3, a new member
of the Rab6 gene family, and multidrug resistance. Biochim. Biophys.
Acta 1589: 112-123, 2002.
4. Tian, K.; Jurukovski, V.; Yuan, L.; Shan, J.; Xu, H.: WTH3, which
encodes a small G protein, is differentially regulated in multidrug-resistant
and sensitive MCF7 cells. Cancer Res. 65: 7421-7428, 2005.
5. Tian, K.; Wang, Y.; Xu, H.: WTH3 is a direct target of the p53
protein. Brit. J. Cancer 96: 1579-1586, 2007.
*FIELD* CD
Patricia A. Hartz: 7/13/2009
*FIELD* ED
mgross: 07/13/2009