Full text data of RPS15
RPS15
(RIG)
[Confidence: low (only semi-automatic identification from reviews)]
40S ribosomal protein S15 (RIG protein)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
40S ribosomal protein S15 (RIG protein)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
P62841
ID RS15_HUMAN Reviewed; 145 AA.
AC P62841; A5D8V9; P11174; Q3KRA1; Q9UDC2;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 2.
DT 22-JAN-2014, entry version 103.
DE RecName: Full=40S ribosomal protein S15;
DE AltName: Full=RIG protein;
GN Name=RPS15; Synonyms=RIG;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2821540; DOI=10.1073/pnas.84.19.6659;
RA Inoue C., Shiga K., Takasawa S., Kitagawa M., Yamamoto H., Okamoto H.;
RT "Evolutionary conservation of the insulinoma gene rig and its possible
RT function.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:6659-6662(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2159154; DOI=10.1073/pnas.87.9.3594;
RA Shiga K., Yamamoto H., Okamoto H.;
RT "Isolation and characterization of the human homologue of rig and its
RT pseudogenes: the functional gene has features characteristic of
RT housekeeping genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:3594-3598(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yu L., Zhao S.Y.;
RT "Cloning and characterization of human insulinoma protein.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 11-145.
RC TISSUE=Brain;
RX PubMed=1466847;
RA Rosenblatt J.D., Tomkins P., Rosenthal M., Kacena A., Chan G.,
RA Valderama R., Harrington W. Jr., Saxton E., Diagne A., Zhao J.-Q.,
RA Mitsuyasu R.T., Weisbart R.H.;
RT "Progressive spastic myelopathy in a patient co-infected with HIV-1
RT and HTLV-II: autoantibodies to the human homologue of rig in blood and
RT cerebrospinal fluid.";
RL AIDS 6:1151-1158(1992).
RN [6]
RP PROTEIN SEQUENCE OF 101-116.
RC TISSUE=Placenta;
RX PubMed=8706699; DOI=10.1111/j.1432-1033.1996.0144u.x;
RA Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K.,
RA Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.;
RT "Characterization of the human small-ribosomal-subunit proteins by N-
RT terminal and internal sequencing, and mass spectrometry.";
RL Eur. J. Biochem. 239:144-149(1996).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [10]
RP STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- INTERACTION:
CC Q9HAU5:UPF2; NbExp=1; IntAct=EBI-372635, EBI-372073;
CC -!- SIMILARITY: Belongs to the ribosomal protein S19P family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; J02984; AAA36036.1; -; mRNA.
DR EMBL; M32405; AAA36568.1; -; Genomic_DNA.
DR EMBL; AF145025; AAP97277.1; -; mRNA.
DR EMBL; BC064908; AAH64908.1; -; mRNA.
DR EMBL; BC105810; AAI05811.1; -; mRNA.
DR EMBL; BC141832; AAI41833.1; -; mRNA.
DR PIR; A35908; R3HU15.
DR RefSeq; NP_001009.1; NM_001018.3.
DR UniGene; Hs.406683; -.
DR PDB; 2ZKQ; EM; 8.70 A; s=1-145.
DR PDB; 3J3A; EM; 5.00 A; P=1-145.
DR PDBsum; 2ZKQ; -.
DR PDBsum; 3J3A; -.
DR ProteinModelPortal; P62841; -.
DR SMR; P62841; 4-130.
DR IntAct; P62841; 8.
DR MINT; MINT-5004408; -.
DR STRING; 9606.ENSP00000233609; -.
DR PhosphoSite; P62841; -.
DR DMDM; 51338641; -.
DR PaxDb; P62841; -.
DR PRIDE; P62841; -.
DR DNASU; 6209; -.
DR Ensembl; ENST00000586686; ENSP00000467676; ENSG00000115268.
DR GeneID; 6209; -.
DR KEGG; hsa:6209; -.
DR UCSC; uc002lsp.1; human.
DR CTD; 6209; -.
DR GeneCards; GC19P001438; -.
DR HGNC; HGNC:10388; RPS15.
DR MIM; 180535; gene.
DR neXtProt; NX_P62841; -.
DR PharmGKB; PA34787; -.
DR eggNOG; COG0185; -.
DR HOGENOM; HOG000111561; -.
DR HOVERGEN; HBG000709; -.
DR InParanoid; P62841; -.
DR KO; K02958; -.
DR OrthoDB; EOG780RPS; -.
DR PhylomeDB; P62841; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_17015; Metabolism of proteins.
DR Reactome; REACT_1762; 3' -UTR-mediated translational regulation.
DR Reactome; REACT_21257; Metabolism of RNA.
DR Reactome; REACT_71; Gene Expression.
DR EvolutionaryTrace; P62841; -.
DR GeneWiki; RPS15; -.
DR GenomeRNAi; 6209; -.
DR NextBio; 24119; -.
DR PMAP-CutDB; P62841; -.
DR PRO; PR:P62841; -.
DR ArrayExpress; P62841; -.
DR Bgee; P62841; -.
DR CleanEx; HS_RPS15; -.
DR Genevestigator; P62841; -.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; NAS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IMP:UniProtKB.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IMP:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; IMP:UniProtKB.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
DR GO; GO:0006414; P:translational elongation; TAS:Reactome.
DR GO; GO:0006413; P:translational initiation; TAS:Reactome.
DR GO; GO:0006415; P:translational termination; TAS:Reactome.
DR GO; GO:0019083; P:viral transcription; TAS:Reactome.
DR Gene3D; 3.30.860.10; -; 1.
DR HAMAP; MF_00531; Ribosomal_S19; 1; -.
DR InterPro; IPR002222; Ribosomal_S19.
DR InterPro; IPR020934; Ribosomal_S19_CS.
DR InterPro; IPR023575; Ribosomal_S19_SF.
DR InterPro; IPR005713; Ribosomal_S19A/S15e.
DR PANTHER; PTHR11880; PTHR11880; 1.
DR Pfam; PF00203; Ribosomal_S19; 1.
DR PIRSF; PIRSF002144; Ribosomal_S19; 1.
DR PRINTS; PR00975; RIBOSOMALS19.
DR SUPFAM; SSF54570; SSF54570; 1.
DR TIGRFAMs; TIGR01025; rpsS_arch; 1.
DR PROSITE; PS00323; RIBOSOMAL_S19; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Complete proteome;
KW Direct protein sequencing; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 145 40S ribosomal protein S15.
FT /FTId=PRO_0000130027.
FT MOD_RES 2 2 N-acetylalanine.
SQ SEQUENCE 145 AA; 17040 MW; CA2C682302C7B387 CRC64;
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL
RKAKKEAPPM EKPEVVKTHL RDMIILPEMV GSMVGVYNGK TFNQVEIKPE MIGHYLGEFS
ITYKPVKHGR PGIGATHSSR FIPLK
//
ID RS15_HUMAN Reviewed; 145 AA.
AC P62841; A5D8V9; P11174; Q3KRA1; Q9UDC2;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 2.
DT 22-JAN-2014, entry version 103.
DE RecName: Full=40S ribosomal protein S15;
DE AltName: Full=RIG protein;
GN Name=RPS15; Synonyms=RIG;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2821540; DOI=10.1073/pnas.84.19.6659;
RA Inoue C., Shiga K., Takasawa S., Kitagawa M., Yamamoto H., Okamoto H.;
RT "Evolutionary conservation of the insulinoma gene rig and its possible
RT function.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:6659-6662(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2159154; DOI=10.1073/pnas.87.9.3594;
RA Shiga K., Yamamoto H., Okamoto H.;
RT "Isolation and characterization of the human homologue of rig and its
RT pseudogenes: the functional gene has features characteristic of
RT housekeeping genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:3594-3598(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yu L., Zhao S.Y.;
RT "Cloning and characterization of human insulinoma protein.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 11-145.
RC TISSUE=Brain;
RX PubMed=1466847;
RA Rosenblatt J.D., Tomkins P., Rosenthal M., Kacena A., Chan G.,
RA Valderama R., Harrington W. Jr., Saxton E., Diagne A., Zhao J.-Q.,
RA Mitsuyasu R.T., Weisbart R.H.;
RT "Progressive spastic myelopathy in a patient co-infected with HIV-1
RT and HTLV-II: autoantibodies to the human homologue of rig in blood and
RT cerebrospinal fluid.";
RL AIDS 6:1151-1158(1992).
RN [6]
RP PROTEIN SEQUENCE OF 101-116.
RC TISSUE=Placenta;
RX PubMed=8706699; DOI=10.1111/j.1432-1033.1996.0144u.x;
RA Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K.,
RA Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.;
RT "Characterization of the human small-ribosomal-subunit proteins by N-
RT terminal and internal sequencing, and mass spectrometry.";
RL Eur. J. Biochem. 239:144-149(1996).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [10]
RP STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- INTERACTION:
CC Q9HAU5:UPF2; NbExp=1; IntAct=EBI-372635, EBI-372073;
CC -!- SIMILARITY: Belongs to the ribosomal protein S19P family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; J02984; AAA36036.1; -; mRNA.
DR EMBL; M32405; AAA36568.1; -; Genomic_DNA.
DR EMBL; AF145025; AAP97277.1; -; mRNA.
DR EMBL; BC064908; AAH64908.1; -; mRNA.
DR EMBL; BC105810; AAI05811.1; -; mRNA.
DR EMBL; BC141832; AAI41833.1; -; mRNA.
DR PIR; A35908; R3HU15.
DR RefSeq; NP_001009.1; NM_001018.3.
DR UniGene; Hs.406683; -.
DR PDB; 2ZKQ; EM; 8.70 A; s=1-145.
DR PDB; 3J3A; EM; 5.00 A; P=1-145.
DR PDBsum; 2ZKQ; -.
DR PDBsum; 3J3A; -.
DR ProteinModelPortal; P62841; -.
DR SMR; P62841; 4-130.
DR IntAct; P62841; 8.
DR MINT; MINT-5004408; -.
DR STRING; 9606.ENSP00000233609; -.
DR PhosphoSite; P62841; -.
DR DMDM; 51338641; -.
DR PaxDb; P62841; -.
DR PRIDE; P62841; -.
DR DNASU; 6209; -.
DR Ensembl; ENST00000586686; ENSP00000467676; ENSG00000115268.
DR GeneID; 6209; -.
DR KEGG; hsa:6209; -.
DR UCSC; uc002lsp.1; human.
DR CTD; 6209; -.
DR GeneCards; GC19P001438; -.
DR HGNC; HGNC:10388; RPS15.
DR MIM; 180535; gene.
DR neXtProt; NX_P62841; -.
DR PharmGKB; PA34787; -.
DR eggNOG; COG0185; -.
DR HOGENOM; HOG000111561; -.
DR HOVERGEN; HBG000709; -.
DR InParanoid; P62841; -.
DR KO; K02958; -.
DR OrthoDB; EOG780RPS; -.
DR PhylomeDB; P62841; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_17015; Metabolism of proteins.
DR Reactome; REACT_1762; 3' -UTR-mediated translational regulation.
DR Reactome; REACT_21257; Metabolism of RNA.
DR Reactome; REACT_71; Gene Expression.
DR EvolutionaryTrace; P62841; -.
DR GeneWiki; RPS15; -.
DR GenomeRNAi; 6209; -.
DR NextBio; 24119; -.
DR PMAP-CutDB; P62841; -.
DR PRO; PR:P62841; -.
DR ArrayExpress; P62841; -.
DR Bgee; P62841; -.
DR CleanEx; HS_RPS15; -.
DR Genevestigator; P62841; -.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; NAS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IMP:UniProtKB.
DR GO; GO:0000056; P:ribosomal small subunit export from nucleus; IMP:UniProtKB.
DR GO; GO:0006364; P:rRNA processing; IMP:UniProtKB.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
DR GO; GO:0006414; P:translational elongation; TAS:Reactome.
DR GO; GO:0006413; P:translational initiation; TAS:Reactome.
DR GO; GO:0006415; P:translational termination; TAS:Reactome.
DR GO; GO:0019083; P:viral transcription; TAS:Reactome.
DR Gene3D; 3.30.860.10; -; 1.
DR HAMAP; MF_00531; Ribosomal_S19; 1; -.
DR InterPro; IPR002222; Ribosomal_S19.
DR InterPro; IPR020934; Ribosomal_S19_CS.
DR InterPro; IPR023575; Ribosomal_S19_SF.
DR InterPro; IPR005713; Ribosomal_S19A/S15e.
DR PANTHER; PTHR11880; PTHR11880; 1.
DR Pfam; PF00203; Ribosomal_S19; 1.
DR PIRSF; PIRSF002144; Ribosomal_S19; 1.
DR PRINTS; PR00975; RIBOSOMALS19.
DR SUPFAM; SSF54570; SSF54570; 1.
DR TIGRFAMs; TIGR01025; rpsS_arch; 1.
DR PROSITE; PS00323; RIBOSOMAL_S19; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Complete proteome;
KW Direct protein sequencing; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 145 40S ribosomal protein S15.
FT /FTId=PRO_0000130027.
FT MOD_RES 2 2 N-acetylalanine.
SQ SEQUENCE 145 AA; 17040 MW; CA2C682302C7B387 CRC64;
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL
RKAKKEAPPM EKPEVVKTHL RDMIILPEMV GSMVGVYNGK TFNQVEIKPE MIGHYLGEFS
ITYKPVKHGR PGIGATHSSR FIPLK
//
MIM
180535
*RECORD*
*FIELD* NO
180535
*FIELD* TI
*180535 RIBOSOMAL PROTEIN S15; RPS15
;;RAT INSULINOMA GENE HOMOLOG; RIG
*FIELD* TX
read moreThe gene called Rig (rat insulinoma gene) was first isolated from a cDNA
library of rat insulinomas. Its cognate gene has been found to be
activated in various human tumors such as insulinomas, esophageal
cancers, and colon cancers. Inoue et al. (1987) isolated a human
insulinoma cDNA encoding the human homolog of Rig. Structural analysis
indicated that the predicted 145-amino acid RIG protein may be a
DNA-binding protein. Shiga et al. (1990) isolated the genomic sequence
of human RIG from a genomic DNA library constructed from a human
esophageal carcinoma and determined its complete nucleotide sequence.
The gene is composed of about 3,000 nucleotides and divided into 4 exons
separated by 3 introns. The transcription initiation site was located
-46 bp upstream from the first ATG codon. Because of CpG islands in the
5-prime region and regions with a high GC content and because of the
wide expression of RIG in tissues and cells, Shiga et al. (1990)
suggested that RIG may belong to the class of 'housekeeping' genes,
whose products are necessary for the growth of all cell types. The human
genome contains at least 6 copies of RIG pseudogenes, 4 of which have
the characteristics of processed pseudogenes. Kitagawa et al. (1991)
demonstrated the normal function of Rig. They showed that the
immunoreactivity to a monoclonal antibody against the deduced Rig
protein and the translation product of Rig mRNA comigrated with
ribosomal protein S15. The amino acid sequence of ribosomal protein S15
purified from rat liver coincided with that deduced from the nucleotide
sequence of Rig mRNA, but there were indications that the initiator
methionine was removed and the succeeding alanyl residue was
monoacetylated. The authors concluded that the product of the Rig gene
is ribosomal protein S15.
By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi
et al. (1998) mapped the human RPS15 gene to 19p.
*FIELD* RF
1. Inoue, C.; Shiga, K.; Takasawa, S.; Kitagawa, M.; Yamamoto, H.;
Okamoto, H.: Evolutionary conservation of the insulinoma gene rig
and its possible function. Proc. Nat. Acad. Sci. 84: 6659-6662,
1987.
2. Kenmochi, N.; Kawaguchi, T.; Rozen, S.; Davis, E.; Goodman, N.;
Hudson, T. J.; Tanaka, T.; Page, D. C.: A map of 75 human ribosomal
protein genes. Genome Res. 8: 509-523, 1998.
3. Kitagawa, M.; Takasawa, S.; Kikuchi, N.; Itoh, T.; Teraoka, H.;
Yamamoto, H.; Okamoto, H.: Rig encodes ribosomal protein S15: the
primary structure of mammalian ribosomal protein S15. FEBS Lett. 283:
210-214, 1991.
4. Shiga, K.; Yamamoto, H.; Okamoto, H.: Isolation and characterization
of the human homologue of rig and its pseudogenes: the functional
gene has features characteristic of housekeeping genes. Proc. Nat.
Acad. Sci. 87: 3594-3598, 1990.
*FIELD* CN
Patti M. Sherman - updated: 3/25/1999
*FIELD* CD
Victor A. McKusick: 10/9/1992
*FIELD* ED
carol: 04/01/1999
terry: 6/18/1998
carol: 10/9/1992
*RECORD*
*FIELD* NO
180535
*FIELD* TI
*180535 RIBOSOMAL PROTEIN S15; RPS15
;;RAT INSULINOMA GENE HOMOLOG; RIG
*FIELD* TX
read moreThe gene called Rig (rat insulinoma gene) was first isolated from a cDNA
library of rat insulinomas. Its cognate gene has been found to be
activated in various human tumors such as insulinomas, esophageal
cancers, and colon cancers. Inoue et al. (1987) isolated a human
insulinoma cDNA encoding the human homolog of Rig. Structural analysis
indicated that the predicted 145-amino acid RIG protein may be a
DNA-binding protein. Shiga et al. (1990) isolated the genomic sequence
of human RIG from a genomic DNA library constructed from a human
esophageal carcinoma and determined its complete nucleotide sequence.
The gene is composed of about 3,000 nucleotides and divided into 4 exons
separated by 3 introns. The transcription initiation site was located
-46 bp upstream from the first ATG codon. Because of CpG islands in the
5-prime region and regions with a high GC content and because of the
wide expression of RIG in tissues and cells, Shiga et al. (1990)
suggested that RIG may belong to the class of 'housekeeping' genes,
whose products are necessary for the growth of all cell types. The human
genome contains at least 6 copies of RIG pseudogenes, 4 of which have
the characteristics of processed pseudogenes. Kitagawa et al. (1991)
demonstrated the normal function of Rig. They showed that the
immunoreactivity to a monoclonal antibody against the deduced Rig
protein and the translation product of Rig mRNA comigrated with
ribosomal protein S15. The amino acid sequence of ribosomal protein S15
purified from rat liver coincided with that deduced from the nucleotide
sequence of Rig mRNA, but there were indications that the initiator
methionine was removed and the succeeding alanyl residue was
monoacetylated. The authors concluded that the product of the Rig gene
is ribosomal protein S15.
By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi
et al. (1998) mapped the human RPS15 gene to 19p.
*FIELD* RF
1. Inoue, C.; Shiga, K.; Takasawa, S.; Kitagawa, M.; Yamamoto, H.;
Okamoto, H.: Evolutionary conservation of the insulinoma gene rig
and its possible function. Proc. Nat. Acad. Sci. 84: 6659-6662,
1987.
2. Kenmochi, N.; Kawaguchi, T.; Rozen, S.; Davis, E.; Goodman, N.;
Hudson, T. J.; Tanaka, T.; Page, D. C.: A map of 75 human ribosomal
protein genes. Genome Res. 8: 509-523, 1998.
3. Kitagawa, M.; Takasawa, S.; Kikuchi, N.; Itoh, T.; Teraoka, H.;
Yamamoto, H.; Okamoto, H.: Rig encodes ribosomal protein S15: the
primary structure of mammalian ribosomal protein S15. FEBS Lett. 283:
210-214, 1991.
4. Shiga, K.; Yamamoto, H.; Okamoto, H.: Isolation and characterization
of the human homologue of rig and its pseudogenes: the functional
gene has features characteristic of housekeeping genes. Proc. Nat.
Acad. Sci. 87: 3594-3598, 1990.
*FIELD* CN
Patti M. Sherman - updated: 3/25/1999
*FIELD* CD
Victor A. McKusick: 10/9/1992
*FIELD* ED
carol: 04/01/1999
terry: 6/18/1998
carol: 10/9/1992