Full text data of RPS20
RPS20
[Confidence: low (only semi-automatic identification from reviews)]
40S ribosomal protein S20
Note: presumably soluble (membrane word is not in UniProt keywords or features)
40S ribosomal protein S20
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
P60866
ID RS20_HUMAN Reviewed; 119 AA.
AC P60866; B2R4F4; B4DW28; P17075; Q5M8S9;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 13-APR-2004, sequence version 1.
DT 22-JAN-2014, entry version 99.
DE RecName: Full=40S ribosomal protein S20;
GN Name=RPS20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8479924; DOI=10.1093/nar/21.7.1672;
RA Chu W., Presky D.H., Swerlick R.A., Burns D.K.;
RT "Human ribosomal protein S20 cDNA sequence.";
RL Nucleic Acids Res. 21:1672-1672(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11875025; DOI=10.1101/gr.214202;
RA Yoshihama M., Uechi T., Asakawa S., Kawasaki K., Kato S., Higa S.,
RA Maeda N., Minoshima S., Tanaka T., Shimizu N., Kenmochi N.;
RT "The human ribosomal protein genes: sequencing and comparative
RT analysis of 73 genes.";
RL Genome Res. 12:379-390(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Synovium, and Thymus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S.,
RA Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A.,
RA Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Allen N.R., Anderson S., Asakawa T., Blechschmidt K., Bloom T.,
RA Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K.,
RA DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G.,
RA Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B.,
RA Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C.,
RA O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K.,
RA Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R.,
RA Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K.,
RA Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q.,
RA Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N.,
RA Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Ovary, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 2-19; 35-41 AND 88-99, CLEAVAGE OF INITIATOR
RP METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
RC TISSUE=Colon adenocarcinoma;
RA Bienvenut W.V., Murray L., Brunton V.G., Frame M.C.;
RL Submitted (JUL-2007) to UniProtKB.
RN [8]
RP PROTEIN SEQUENCE OF 9-24.
RC TISSUE=Placenta;
RX PubMed=8706699; DOI=10.1111/j.1432-1033.1996.0144u.x;
RA Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K.,
RA Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.;
RT "Characterization of the human small-ribosomal-subunit proteins by N-
RT terminal and internal sequencing, and mass spectrometry.";
RL Eur. J. Biochem. 239:144-149(1996).
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 51-110.
RX PubMed=9582194;
RA Kenmochi N., Kawaguchi T., Rozen S., Davis E., Goodman N.,
RA Hudson T.J., Tanaka T., Page D.C.;
RT "A map of 75 human ribosomal protein genes.";
RL Genome Res. 8:509-523(1998).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-9, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- SUBUNIT: Component of the 40S small ribosomal subunit (By
CC similarity).
CC -!- INTERACTION:
CC Q15843:NEDD8; NbExp=2; IntAct=EBI-353105, EBI-716247;
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P60866-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P60866-2; Sequence=VSP_042724;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the ribosomal protein S10P family.
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DR EMBL; L06498; AAA60286.1; -; mRNA.
DR EMBL; AB061842; BAB79480.1; -; Genomic_DNA.
DR EMBL; AK301342; BAG62890.1; -; mRNA.
DR EMBL; AK311808; BAG34751.1; -; mRNA.
DR EMBL; AC107376; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471068; EAW86771.1; -; Genomic_DNA.
DR EMBL; CH471068; EAW86772.1; -; Genomic_DNA.
DR EMBL; BC007507; AAH07507.1; -; mRNA.
DR EMBL; BC087850; AAH87850.1; -; mRNA.
DR EMBL; AB007156; BAA25820.1; -; Genomic_DNA.
DR PIR; S33710; S33710.
DR RefSeq; NP_001014.1; NM_001023.3.
DR RefSeq; NP_001139699.1; NM_001146227.1.
DR UniGene; Hs.8102; -.
DR PDB; 3J3A; EM; 5.00 A; U=1-119.
DR PDBsum; 3J3A; -.
DR ProteinModelPortal; P60866; -.
DR SMR; P60866; 16-119.
DR IntAct; P60866; 24.
DR MINT; MINT-5000180; -.
DR STRING; 9606.ENSP00000009589; -.
DR PhosphoSite; P60866; -.
DR DMDM; 46397703; -.
DR PaxDb; P60866; -.
DR PRIDE; P60866; -.
DR DNASU; 6224; -.
DR Ensembl; ENST00000009589; ENSP00000009589; ENSG00000008988.
DR Ensembl; ENST00000519807; ENSP00000429374; ENSG00000008988.
DR Ensembl; ENST00000521262; ENSP00000427788; ENSG00000008988.
DR GeneID; 6224; -.
DR KEGG; hsa:6224; -.
DR UCSC; uc003xsn.2; human.
DR CTD; 6224; -.
DR GeneCards; GC08M056982; -.
DR HGNC; HGNC:10405; RPS20.
DR HPA; HPA003570; -.
DR MIM; 603682; gene.
DR neXtProt; NX_P60866; -.
DR PharmGKB; PA34807; -.
DR eggNOG; COG0051; -.
DR HOGENOM; HOG000270245; -.
DR HOVERGEN; HBG004448; -.
DR InParanoid; P60866; -.
DR KO; K02969; -.
DR OMA; YELKIHK; -.
DR OrthoDB; EOG7KH9MQ; -.
DR PhylomeDB; P60866; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_17015; Metabolism of proteins.
DR Reactome; REACT_1762; 3' -UTR-mediated translational regulation.
DR Reactome; REACT_21257; Metabolism of RNA.
DR Reactome; REACT_71; Gene Expression.
DR ChiTaRS; RPS20; human.
DR GeneWiki; RPS20; -.
DR GenomeRNAi; 6224; -.
DR NextBio; 24163; -.
DR PRO; PR:P60866; -.
DR ArrayExpress; P60866; -.
DR Bgee; P60866; -.
DR CleanEx; HS_RPS20; -.
DR Genevestigator; P60866; -.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; NAS:UniProtKB.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:UniProtKB.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
DR GO; GO:0006414; P:translational elongation; TAS:Reactome.
DR GO; GO:0006413; P:translational initiation; TAS:Reactome.
DR GO; GO:0006415; P:translational termination; TAS:Reactome.
DR GO; GO:0019083; P:viral transcription; TAS:Reactome.
DR Gene3D; 3.30.70.600; -; 1.
DR InterPro; IPR001848; Ribosomal_S10.
DR InterPro; IPR018268; Ribosomal_S10_CS.
DR InterPro; IPR027486; Ribosomal_S10_dom.
DR InterPro; IPR005729; Ribosomal_S10_euk/arc.
DR PANTHER; PTHR11700; PTHR11700; 1.
DR Pfam; PF00338; Ribosomal_S10; 1.
DR PRINTS; PR00971; RIBOSOMALS10.
DR SUPFAM; SSF54999; SSF54999; 1.
DR TIGRFAMs; TIGR01046; S10_Arc_S20_Euk; 1.
DR PROSITE; PS00361; RIBOSOMAL_S10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Complete proteome;
KW Cytoplasm; Direct protein sequencing; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW Ubl conjugation.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 119 40S ribosomal protein S20.
FT /FTId=PRO_0000146683.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 9 9 Phosphothreonine.
FT CROSSLNK 8 8 Glycyl lysine isopeptide (Lys-Gly)
FT (interchain with G-Cter in ubiquitin).
FT VAR_SEQ 112 119 VEVTIADA -> LIESTDAEPMDTEGQQYTLRSVFESPGTC
FT PF (in isoform 2).
FT /FTId=VSP_042724.
SQ SEQUENCE 119 AA; 13373 MW; 8802DF7894ADDF94 CRC64;
MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT
LRITTRKTPC GEGSKTWDRF QMRIHKRLID LHSPSEIVKQ ITSISIEPGV EVEVTIADA
//
ID RS20_HUMAN Reviewed; 119 AA.
AC P60866; B2R4F4; B4DW28; P17075; Q5M8S9;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 13-APR-2004, sequence version 1.
DT 22-JAN-2014, entry version 99.
DE RecName: Full=40S ribosomal protein S20;
GN Name=RPS20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8479924; DOI=10.1093/nar/21.7.1672;
RA Chu W., Presky D.H., Swerlick R.A., Burns D.K.;
RT "Human ribosomal protein S20 cDNA sequence.";
RL Nucleic Acids Res. 21:1672-1672(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11875025; DOI=10.1101/gr.214202;
RA Yoshihama M., Uechi T., Asakawa S., Kawasaki K., Kato S., Higa S.,
RA Maeda N., Minoshima S., Tanaka T., Shimizu N., Kenmochi N.;
RT "The human ribosomal protein genes: sequencing and comparative
RT analysis of 73 genes.";
RL Genome Res. 12:379-390(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Synovium, and Thymus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S.,
RA Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A.,
RA Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Allen N.R., Anderson S., Asakawa T., Blechschmidt K., Bloom T.,
RA Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K.,
RA DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G.,
RA Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B.,
RA Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C.,
RA O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K.,
RA Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R.,
RA Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K.,
RA Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q.,
RA Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N.,
RA Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Ovary, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PROTEIN SEQUENCE OF 2-19; 35-41 AND 88-99, CLEAVAGE OF INITIATOR
RP METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
RC TISSUE=Colon adenocarcinoma;
RA Bienvenut W.V., Murray L., Brunton V.G., Frame M.C.;
RL Submitted (JUL-2007) to UniProtKB.
RN [8]
RP PROTEIN SEQUENCE OF 9-24.
RC TISSUE=Placenta;
RX PubMed=8706699; DOI=10.1111/j.1432-1033.1996.0144u.x;
RA Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K.,
RA Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.;
RT "Characterization of the human small-ribosomal-subunit proteins by N-
RT terminal and internal sequencing, and mass spectrometry.";
RL Eur. J. Biochem. 239:144-149(1996).
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 51-110.
RX PubMed=9582194;
RA Kenmochi N., Kawaguchi T., Rozen S., Davis E., Goodman N.,
RA Hudson T.J., Tanaka T., Page D.C.;
RT "A map of 75 human ribosomal protein genes.";
RL Genome Res. 8:509-523(1998).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-9, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- SUBUNIT: Component of the 40S small ribosomal subunit (By
CC similarity).
CC -!- INTERACTION:
CC Q15843:NEDD8; NbExp=2; IntAct=EBI-353105, EBI-716247;
CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P60866-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P60866-2; Sequence=VSP_042724;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the ribosomal protein S10P family.
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DR EMBL; L06498; AAA60286.1; -; mRNA.
DR EMBL; AB061842; BAB79480.1; -; Genomic_DNA.
DR EMBL; AK301342; BAG62890.1; -; mRNA.
DR EMBL; AK311808; BAG34751.1; -; mRNA.
DR EMBL; AC107376; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471068; EAW86771.1; -; Genomic_DNA.
DR EMBL; CH471068; EAW86772.1; -; Genomic_DNA.
DR EMBL; BC007507; AAH07507.1; -; mRNA.
DR EMBL; BC087850; AAH87850.1; -; mRNA.
DR EMBL; AB007156; BAA25820.1; -; Genomic_DNA.
DR PIR; S33710; S33710.
DR RefSeq; NP_001014.1; NM_001023.3.
DR RefSeq; NP_001139699.1; NM_001146227.1.
DR UniGene; Hs.8102; -.
DR PDB; 3J3A; EM; 5.00 A; U=1-119.
DR PDBsum; 3J3A; -.
DR ProteinModelPortal; P60866; -.
DR SMR; P60866; 16-119.
DR IntAct; P60866; 24.
DR MINT; MINT-5000180; -.
DR STRING; 9606.ENSP00000009589; -.
DR PhosphoSite; P60866; -.
DR DMDM; 46397703; -.
DR PaxDb; P60866; -.
DR PRIDE; P60866; -.
DR DNASU; 6224; -.
DR Ensembl; ENST00000009589; ENSP00000009589; ENSG00000008988.
DR Ensembl; ENST00000519807; ENSP00000429374; ENSG00000008988.
DR Ensembl; ENST00000521262; ENSP00000427788; ENSG00000008988.
DR GeneID; 6224; -.
DR KEGG; hsa:6224; -.
DR UCSC; uc003xsn.2; human.
DR CTD; 6224; -.
DR GeneCards; GC08M056982; -.
DR HGNC; HGNC:10405; RPS20.
DR HPA; HPA003570; -.
DR MIM; 603682; gene.
DR neXtProt; NX_P60866; -.
DR PharmGKB; PA34807; -.
DR eggNOG; COG0051; -.
DR HOGENOM; HOG000270245; -.
DR HOVERGEN; HBG004448; -.
DR InParanoid; P60866; -.
DR KO; K02969; -.
DR OMA; YELKIHK; -.
DR OrthoDB; EOG7KH9MQ; -.
DR PhylomeDB; P60866; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_17015; Metabolism of proteins.
DR Reactome; REACT_1762; 3' -UTR-mediated translational regulation.
DR Reactome; REACT_21257; Metabolism of RNA.
DR Reactome; REACT_71; Gene Expression.
DR ChiTaRS; RPS20; human.
DR GeneWiki; RPS20; -.
DR GenomeRNAi; 6224; -.
DR NextBio; 24163; -.
DR PRO; PR:P60866; -.
DR ArrayExpress; P60866; -.
DR Bgee; P60866; -.
DR CleanEx; HS_RPS20; -.
DR Genevestigator; P60866; -.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; NAS:UniProtKB.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:UniProtKB.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
DR GO; GO:0006414; P:translational elongation; TAS:Reactome.
DR GO; GO:0006413; P:translational initiation; TAS:Reactome.
DR GO; GO:0006415; P:translational termination; TAS:Reactome.
DR GO; GO:0019083; P:viral transcription; TAS:Reactome.
DR Gene3D; 3.30.70.600; -; 1.
DR InterPro; IPR001848; Ribosomal_S10.
DR InterPro; IPR018268; Ribosomal_S10_CS.
DR InterPro; IPR027486; Ribosomal_S10_dom.
DR InterPro; IPR005729; Ribosomal_S10_euk/arc.
DR PANTHER; PTHR11700; PTHR11700; 1.
DR Pfam; PF00338; Ribosomal_S10; 1.
DR PRINTS; PR00971; RIBOSOMALS10.
DR SUPFAM; SSF54999; SSF54999; 1.
DR TIGRFAMs; TIGR01046; S10_Arc_S20_Euk; 1.
DR PROSITE; PS00361; RIBOSOMAL_S10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Alternative splicing; Complete proteome;
KW Cytoplasm; Direct protein sequencing; Isopeptide bond; Phosphoprotein;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW Ubl conjugation.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 119 40S ribosomal protein S20.
FT /FTId=PRO_0000146683.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 9 9 Phosphothreonine.
FT CROSSLNK 8 8 Glycyl lysine isopeptide (Lys-Gly)
FT (interchain with G-Cter in ubiquitin).
FT VAR_SEQ 112 119 VEVTIADA -> LIESTDAEPMDTEGQQYTLRSVFESPGTC
FT PF (in isoform 2).
FT /FTId=VSP_042724.
SQ SEQUENCE 119 AA; 13373 MW; 8802DF7894ADDF94 CRC64;
MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT
LRITTRKTPC GEGSKTWDRF QMRIHKRLID LHSPSEIVKQ ITSISIEPGV EVEVTIADA
//
MIM
603682
*RECORD*
*FIELD* NO
603682
*FIELD* TI
*603682 RIBOSOMAL PROTEIN S20; RPS20
*FIELD* TX
The mammalian ribosome is composed of 4 RNA species (see 180450) and
read moreapproximately 80 different proteins.
Chu et al. (1993) isolated a human dermal vascular endothelial cell cDNA
encoding ribosomal protein S20 (RPS20). The deduced 119-amino acid human
RPS20 protein is identical to rat Rps20. Northern blot analysis detected
an approximately 650-bp RPS20 transcript. Southern blot analysis showed
that the RPS20 gene may be a member of a multigene family.
By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi
et al. (1998) mapped the human RPS20 gene to 8q.
ANIMAL MODEL
McGowan et al. (2008) reported 2 mouse 'dark skin' loci, Dsk3 and Dsk4,
caused by mutations in Rps19 (603474) and Rps20, respectively. These
mice have dark paws, tail skin, and ears, with melanocytosis limited to
the epidermis. In the model proposed by McGowan et al. (2008), reduced
dosage of Rps6 (180460), Rps19, or Rps20 triggers stabilization and/or
activation of p53 (191170), which gives rise to a pleiotropic phenotype
whose components depend on the sensitivity and response of individual
cell types and on specific downstream targets of p53. Stabilization of
p53 stimulates Kit ligand (KITLG; 184745) expression and, consequently,
epidermal melanocytosis via a paracrine mechanism. Increased apoptosis
causes erythrocyte hypoplasia and anemia, and activation of p53 causes
reduced growth and decreased body size. McGowan et al. (2008) concluded
that their results provide a mechanistic explanation for the diverse
collection of phenotypes that accompany reduced dosage of genes encoding
ribosomal proteins, and have implications for understanding normal human
variation and human disease.
*FIELD* RF
1. Chu, W.; Presky, D. H.; Swerlick, R. A.; Burns, D. K.: Human ribosomal
protein S20 cDNA sequence. Nucleic Acids Res. 21: 1672 only, 1993.
2. Kenmochi, N.; Kawaguchi, T.; Rozen, S.; Davis, E.; Goodman, N.;
Hudson, T. J.; Tanaka, T.; Page, D. C.: A map of 75 human ribosomal
protein genes. Genome Res. 8: 509-523, 1998.
3. McGowan, K. A.; Li, J. Z.; Park, C. Y.; Beaudry, V.; Tabor, H.
K.; Sabnis, A. J.; Zhang, W.; Fuchs, H.; de Angelis, M. H.; Myers,
R. M.; Attardi, L. D.; Barsh, G. S.: Ribosomal mutations cause p53-mediated
dark skin and pleiotropic effects. Nature Genet. 40: 963-970, 2008.
*FIELD* CN
Ada Hamosh - updated: 10/24/2008
*FIELD* CD
Patti M. Sherman: 3/30/1999
*FIELD* ED
alopez: 11/10/2008
terry: 10/24/2008
carol: 3/31/1999
*RECORD*
*FIELD* NO
603682
*FIELD* TI
*603682 RIBOSOMAL PROTEIN S20; RPS20
*FIELD* TX
The mammalian ribosome is composed of 4 RNA species (see 180450) and
read moreapproximately 80 different proteins.
Chu et al. (1993) isolated a human dermal vascular endothelial cell cDNA
encoding ribosomal protein S20 (RPS20). The deduced 119-amino acid human
RPS20 protein is identical to rat Rps20. Northern blot analysis detected
an approximately 650-bp RPS20 transcript. Southern blot analysis showed
that the RPS20 gene may be a member of a multigene family.
By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi
et al. (1998) mapped the human RPS20 gene to 8q.
ANIMAL MODEL
McGowan et al. (2008) reported 2 mouse 'dark skin' loci, Dsk3 and Dsk4,
caused by mutations in Rps19 (603474) and Rps20, respectively. These
mice have dark paws, tail skin, and ears, with melanocytosis limited to
the epidermis. In the model proposed by McGowan et al. (2008), reduced
dosage of Rps6 (180460), Rps19, or Rps20 triggers stabilization and/or
activation of p53 (191170), which gives rise to a pleiotropic phenotype
whose components depend on the sensitivity and response of individual
cell types and on specific downstream targets of p53. Stabilization of
p53 stimulates Kit ligand (KITLG; 184745) expression and, consequently,
epidermal melanocytosis via a paracrine mechanism. Increased apoptosis
causes erythrocyte hypoplasia and anemia, and activation of p53 causes
reduced growth and decreased body size. McGowan et al. (2008) concluded
that their results provide a mechanistic explanation for the diverse
collection of phenotypes that accompany reduced dosage of genes encoding
ribosomal proteins, and have implications for understanding normal human
variation and human disease.
*FIELD* RF
1. Chu, W.; Presky, D. H.; Swerlick, R. A.; Burns, D. K.: Human ribosomal
protein S20 cDNA sequence. Nucleic Acids Res. 21: 1672 only, 1993.
2. Kenmochi, N.; Kawaguchi, T.; Rozen, S.; Davis, E.; Goodman, N.;
Hudson, T. J.; Tanaka, T.; Page, D. C.: A map of 75 human ribosomal
protein genes. Genome Res. 8: 509-523, 1998.
3. McGowan, K. A.; Li, J. Z.; Park, C. Y.; Beaudry, V.; Tabor, H.
K.; Sabnis, A. J.; Zhang, W.; Fuchs, H.; de Angelis, M. H.; Myers,
R. M.; Attardi, L. D.; Barsh, G. S.: Ribosomal mutations cause p53-mediated
dark skin and pleiotropic effects. Nature Genet. 40: 963-970, 2008.
*FIELD* CN
Ada Hamosh - updated: 10/24/2008
*FIELD* CD
Patti M. Sherman: 3/30/1999
*FIELD* ED
alopez: 11/10/2008
terry: 10/24/2008
carol: 3/31/1999