Full text data of RPS4X
RPS4X
(CCG2, RPS4, SCAR)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
40S ribosomal protein S4, X isoform (SCR10; Single copy abundant mRNA protein)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
40S ribosomal protein S4, X isoform (SCR10; Single copy abundant mRNA protein)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
P62701
ID RS4X_HUMAN Reviewed; 263 AA.
AC P62701; P12631; P12750; P27576; P55831; Q14727; Q6IPY4;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 2.
DT 22-JAN-2014, entry version 101.
DE RecName: Full=40S ribosomal protein S4, X isoform;
DE AltName: Full=SCR10;
DE AltName: Full=Single copy abundant mRNA protein;
GN Name=RPS4X; Synonyms=CCG2, RPS4, SCAR;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2124517; DOI=10.1016/0092-8674(90)90416-C;
RA Fisher E.M.C., Beer-Romero P., Brown L.G., Ridley A., McNeil J.A.,
RA Lawrence J.B., Willard H.F., Bieber F.R., Page D.C.;
RT "Homologous ribosomal protein genes on the human X and Y chromosomes:
RT escape from X inactivation and possible implications for Turner
RT syndrome.";
RL Cell 63:1205-1218(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1795030;
RA Watanabe M., Furuno N., Goebl M., Go M., Miyauchi K., Sekiguchi T.,
RA Basilico C., Nishimoto T.;
RT "Molecular cloning of the human gene, CCG2, that complements the BHK-
RT derived temperature-sensitive cell cycle mutant tsBN63: identity of
RT CCG2 with the human X chromosomal SCAR/RPS4X gene.";
RL J. Cell Sci. 100:35-43(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Zuo L., Baybayan P., Kuang W.-J., Brown L., Page D., Chen E.;
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-59.
RC TISSUE=Brain;
RA Dmitrenko V.V., Garifulin O.M., Kavsan V.M.;
RT "Characterization of different mRNA types expressed in human brain.";
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP PROTEIN SEQUENCE OF 2-11.
RC TISSUE=Placenta;
RX PubMed=8706699; DOI=10.1111/j.1432-1033.1996.0144u.x;
RA Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K.,
RA Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.;
RT "Characterization of the human small-ribosomal-subunit proteins by N-
RT terminal and internal sequencing, and mass spectrometry.";
RL Eur. J. Biochem. 239:144-149(1996).
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 19-263.
RX PubMed=2829364; DOI=10.1007/BF01535047;
RA Wiles M.V., Alexander C.M., Goodfellow P.N.;
RT "Isolation of an abundantly expressed sequence from the human X
RT chromosome by differential screening.";
RL Somat. Cell Mol. Genet. 14:31-39(1988).
RN [9]
RP IDENTIFICATION IN A MRNP GRANULE COMPLEX, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=17289661; DOI=10.1074/mcp.M600346-MCP200;
RA Joeson L., Vikesaa J., Krogh A., Nielsen L.K., Hansen T., Borup R.,
RA Johnsen A.H., Christiansen J., Nielsen F.C.;
RT "Molecular composition of IMP1 ribonucleoprotein granules.";
RL Mol. Cell. Proteomics 6:798-811(2007).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- SUBUNIT: Identified in a IGF2BP1-dependent mRNP granule complex
CC containing untranslated mRNAs.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Note=Localized in cytoplasmic
CC mRNP granules containing untranslated mRNAs.
CC -!- SIMILARITY: Belongs to the ribosomal protein S4e family.
CC -!- SIMILARITY: Contains 1 S4 RNA-binding domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M58458; AAA63255.1; -; mRNA.
DR EMBL; AF041428; AAB96968.1; -; Genomic_DNA.
DR EMBL; CR456735; CAG33016.1; -; mRNA.
DR EMBL; BC000472; AAH00472.1; -; mRNA.
DR EMBL; BC071662; AAH71662.1; -; mRNA.
DR EMBL; BC100903; AAI00904.1; -; mRNA.
DR EMBL; BC100904; AAI00905.1; -; mRNA.
DR EMBL; Z70767; CAA94808.1; -; mRNA.
DR EMBL; M22146; AAA36597.1; -; mRNA.
DR PIR; B36338; R3HU4X.
DR RefSeq; NP_000998.1; NM_001007.4.
DR UniGene; Hs.118076; -.
DR PDB; 3J3A; EM; 5.00 A; E=1-263.
DR PDBsum; 3J3A; -.
DR ProteinModelPortal; P62701; -.
DR SMR; P62701; 1-263.
DR IntAct; P62701; 27.
DR MINT; MINT-1159032; -.
DR STRING; 9606.ENSP00000362744; -.
DR PhosphoSite; P62701; -.
DR DMDM; 50403628; -.
DR SWISS-2DPAGE; P62701; -.
DR PaxDb; P62701; -.
DR PeptideAtlas; P62701; -.
DR PRIDE; P62701; -.
DR DNASU; 6191; -.
DR Ensembl; ENST00000316084; ENSP00000362744; ENSG00000198034.
DR GeneID; 6191; -.
DR KEGG; hsa:6191; -.
DR UCSC; uc004ear.3; human.
DR CTD; 6191; -.
DR GeneCards; GC0XM071475; -.
DR HGNC; HGNC:10424; RPS4X.
DR HPA; HPA000857; -.
DR MIM; 312760; gene.
DR neXtProt; NX_P62701; -.
DR PharmGKB; PA34839; -.
DR eggNOG; COG1471; -.
DR HOVERGEN; HBG000935; -.
DR InParanoid; P62701; -.
DR KO; K02987; -.
DR OMA; GHIQLNL; -.
DR OrthoDB; EOG77WWD1; -.
DR PhylomeDB; P62701; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_17015; Metabolism of proteins.
DR Reactome; REACT_1762; 3' -UTR-mediated translational regulation.
DR Reactome; REACT_21257; Metabolism of RNA.
DR Reactome; REACT_71; Gene Expression.
DR ChiTaRS; RPS4X; human.
DR GeneWiki; RPS4X; -.
DR GenomeRNAi; 6191; -.
DR NextBio; 24041; -.
DR PMAP-CutDB; P62701; -.
DR PRO; PR:P62701; -.
DR ArrayExpress; P62701; -.
DR Bgee; P62701; -.
DR CleanEx; HS_RPS4X; -.
DR Genevestigator; P62701; -.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0005844; C:polysome; IDA:UniProtKB.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IMP:UniProtKB.
DR GO; GO:0007275; P:multicellular organismal development; IMP:UniProtKB.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
DR GO; GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB.
DR GO; GO:0045727; P:positive regulation of translation; IMP:UniProtKB.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
DR GO; GO:0006414; P:translational elongation; TAS:Reactome.
DR GO; GO:0006413; P:translational initiation; TAS:Reactome.
DR GO; GO:0006415; P:translational termination; TAS:Reactome.
DR GO; GO:0019083; P:viral transcription; TAS:Reactome.
DR HAMAP; MF_00485; Ribosomal_S4e; 1; -.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR000876; Ribosomal_S4e.
DR InterPro; IPR013845; Ribosomal_S4e_central_region.
DR InterPro; IPR013843; Ribosomal_S4e_N.
DR InterPro; IPR018199; Ribosomal_S4e_N_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR PANTHER; PTHR11581; PTHR11581; 1.
DR Pfam; PF00467; KOW; 1.
DR Pfam; PF00900; Ribosomal_S4e; 1.
DR Pfam; PF08071; RS4NT; 1.
DR Pfam; PF01479; S4; 1.
DR PIRSF; PIRSF002116; Ribosomal_S4; 1.
DR ProDom; PD002667; Ribosomal_S4e_central; 1.
DR SMART; SM00363; S4; 1.
DR PROSITE; PS00528; RIBOSOMAL_S4E; 1.
DR PROSITE; PS50889; S4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 263 40S ribosomal protein S4, X isoform.
FT /FTId=PRO_0000130805.
FT DOMAIN 42 104 S4 RNA-binding.
FT CONFLICT 33 33 Missing (in Ref. 8; AAA36597).
FT CONFLICT 57 59 TGD -> DRR (in Ref. 6; CAA94808).
SQ SEQUENCE 263 AA; 29598 MW; 87200E545A8958B0 CRC64;
MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE
VKKICMQRFI KIDGKVRTDI TYPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK
YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI QIDLETGKIT DFIKFDTGNL
CMVTGGANLG RIGVITNRER HPGSFDVVHV KDANGNSFAT RLSNIFVIGK GNKPWISLPR
GKGIRLTIAE ERDKRLAAKQ SSG
//
ID RS4X_HUMAN Reviewed; 263 AA.
AC P62701; P12631; P12750; P27576; P55831; Q14727; Q6IPY4;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 2.
DT 22-JAN-2014, entry version 101.
DE RecName: Full=40S ribosomal protein S4, X isoform;
DE AltName: Full=SCR10;
DE AltName: Full=Single copy abundant mRNA protein;
GN Name=RPS4X; Synonyms=CCG2, RPS4, SCAR;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2124517; DOI=10.1016/0092-8674(90)90416-C;
RA Fisher E.M.C., Beer-Romero P., Brown L.G., Ridley A., McNeil J.A.,
RA Lawrence J.B., Willard H.F., Bieber F.R., Page D.C.;
RT "Homologous ribosomal protein genes on the human X and Y chromosomes:
RT escape from X inactivation and possible implications for Turner
RT syndrome.";
RL Cell 63:1205-1218(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1795030;
RA Watanabe M., Furuno N., Goebl M., Go M., Miyauchi K., Sekiguchi T.,
RA Basilico C., Nishimoto T.;
RT "Molecular cloning of the human gene, CCG2, that complements the BHK-
RT derived temperature-sensitive cell cycle mutant tsBN63: identity of
RT CCG2 with the human X chromosomal SCAR/RPS4X gene.";
RL J. Cell Sci. 100:35-43(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Zuo L., Baybayan P., Kuang W.-J., Brown L., Page D., Chen E.;
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-59.
RC TISSUE=Brain;
RA Dmitrenko V.V., Garifulin O.M., Kavsan V.M.;
RT "Characterization of different mRNA types expressed in human brain.";
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP PROTEIN SEQUENCE OF 2-11.
RC TISSUE=Placenta;
RX PubMed=8706699; DOI=10.1111/j.1432-1033.1996.0144u.x;
RA Vladimirov S.N., Ivanov A.V., Karpova G.G., Musolyamov A.K.,
RA Egorov T.A., Thiede B., Wittmann-Liebold B., Otto A.;
RT "Characterization of the human small-ribosomal-subunit proteins by N-
RT terminal and internal sequencing, and mass spectrometry.";
RL Eur. J. Biochem. 239:144-149(1996).
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 19-263.
RX PubMed=2829364; DOI=10.1007/BF01535047;
RA Wiles M.V., Alexander C.M., Goodfellow P.N.;
RT "Isolation of an abundantly expressed sequence from the human X
RT chromosome by differential screening.";
RL Somat. Cell Mol. Genet. 14:31-39(1988).
RN [9]
RP IDENTIFICATION IN A MRNP GRANULE COMPLEX, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=17289661; DOI=10.1074/mcp.M600346-MCP200;
RA Joeson L., Vikesaa J., Krogh A., Nielsen L.K., Hansen T., Borup R.,
RA Johnsen A.H., Christiansen J., Nielsen F.C.;
RT "Molecular composition of IMP1 ribonucleoprotein granules.";
RL Mol. Cell. Proteomics 6:798-811(2007).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP STRUCTURE BY ELECTRON MICROSCOPY (5.0 ANGSTROMS).
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- SUBUNIT: Identified in a IGF2BP1-dependent mRNP granule complex
CC containing untranslated mRNAs.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Note=Localized in cytoplasmic
CC mRNP granules containing untranslated mRNAs.
CC -!- SIMILARITY: Belongs to the ribosomal protein S4e family.
CC -!- SIMILARITY: Contains 1 S4 RNA-binding domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; M58458; AAA63255.1; -; mRNA.
DR EMBL; AF041428; AAB96968.1; -; Genomic_DNA.
DR EMBL; CR456735; CAG33016.1; -; mRNA.
DR EMBL; BC000472; AAH00472.1; -; mRNA.
DR EMBL; BC071662; AAH71662.1; -; mRNA.
DR EMBL; BC100903; AAI00904.1; -; mRNA.
DR EMBL; BC100904; AAI00905.1; -; mRNA.
DR EMBL; Z70767; CAA94808.1; -; mRNA.
DR EMBL; M22146; AAA36597.1; -; mRNA.
DR PIR; B36338; R3HU4X.
DR RefSeq; NP_000998.1; NM_001007.4.
DR UniGene; Hs.118076; -.
DR PDB; 3J3A; EM; 5.00 A; E=1-263.
DR PDBsum; 3J3A; -.
DR ProteinModelPortal; P62701; -.
DR SMR; P62701; 1-263.
DR IntAct; P62701; 27.
DR MINT; MINT-1159032; -.
DR STRING; 9606.ENSP00000362744; -.
DR PhosphoSite; P62701; -.
DR DMDM; 50403628; -.
DR SWISS-2DPAGE; P62701; -.
DR PaxDb; P62701; -.
DR PeptideAtlas; P62701; -.
DR PRIDE; P62701; -.
DR DNASU; 6191; -.
DR Ensembl; ENST00000316084; ENSP00000362744; ENSG00000198034.
DR GeneID; 6191; -.
DR KEGG; hsa:6191; -.
DR UCSC; uc004ear.3; human.
DR CTD; 6191; -.
DR GeneCards; GC0XM071475; -.
DR HGNC; HGNC:10424; RPS4X.
DR HPA; HPA000857; -.
DR MIM; 312760; gene.
DR neXtProt; NX_P62701; -.
DR PharmGKB; PA34839; -.
DR eggNOG; COG1471; -.
DR HOVERGEN; HBG000935; -.
DR InParanoid; P62701; -.
DR KO; K02987; -.
DR OMA; GHIQLNL; -.
DR OrthoDB; EOG77WWD1; -.
DR PhylomeDB; P62701; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_17015; Metabolism of proteins.
DR Reactome; REACT_1762; 3' -UTR-mediated translational regulation.
DR Reactome; REACT_21257; Metabolism of RNA.
DR Reactome; REACT_71; Gene Expression.
DR ChiTaRS; RPS4X; human.
DR GeneWiki; RPS4X; -.
DR GenomeRNAi; 6191; -.
DR NextBio; 24041; -.
DR PMAP-CutDB; P62701; -.
DR PRO; PR:P62701; -.
DR ArrayExpress; P62701; -.
DR Bgee; P62701; -.
DR CleanEx; HS_RPS4X; -.
DR Genevestigator; P62701; -.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
DR GO; GO:0005844; C:polysome; IDA:UniProtKB.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003735; F:structural constituent of ribosome; IMP:UniProtKB.
DR GO; GO:0007275; P:multicellular organismal development; IMP:UniProtKB.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
DR GO; GO:0008284; P:positive regulation of cell proliferation; IMP:UniProtKB.
DR GO; GO:0045727; P:positive regulation of translation; IMP:UniProtKB.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
DR GO; GO:0006414; P:translational elongation; TAS:Reactome.
DR GO; GO:0006413; P:translational initiation; TAS:Reactome.
DR GO; GO:0006415; P:translational termination; TAS:Reactome.
DR GO; GO:0019083; P:viral transcription; TAS:Reactome.
DR HAMAP; MF_00485; Ribosomal_S4e; 1; -.
DR InterPro; IPR005824; KOW.
DR InterPro; IPR000876; Ribosomal_S4e.
DR InterPro; IPR013845; Ribosomal_S4e_central_region.
DR InterPro; IPR013843; Ribosomal_S4e_N.
DR InterPro; IPR018199; Ribosomal_S4e_N_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR PANTHER; PTHR11581; PTHR11581; 1.
DR Pfam; PF00467; KOW; 1.
DR Pfam; PF00900; Ribosomal_S4e; 1.
DR Pfam; PF08071; RS4NT; 1.
DR Pfam; PF01479; S4; 1.
DR PIRSF; PIRSF002116; Ribosomal_S4; 1.
DR ProDom; PD002667; Ribosomal_S4e_central; 1.
DR SMART; SM00363; S4; 1.
DR PROSITE; PS00528; RIBOSOMAL_S4E; 1.
DR PROSITE; PS50889; S4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 263 40S ribosomal protein S4, X isoform.
FT /FTId=PRO_0000130805.
FT DOMAIN 42 104 S4 RNA-binding.
FT CONFLICT 33 33 Missing (in Ref. 8; AAA36597).
FT CONFLICT 57 59 TGD -> DRR (in Ref. 6; CAA94808).
SQ SEQUENCE 263 AA; 29598 MW; 87200E545A8958B0 CRC64;
MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE
VKKICMQRFI KIDGKVRTDI TYPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK
YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKVNDTI QIDLETGKIT DFIKFDTGNL
CMVTGGANLG RIGVITNRER HPGSFDVVHV KDANGNSFAT RLSNIFVIGK GNKPWISLPR
GKGIRLTIAE ERDKRLAAKQ SSG
//
MIM
312760
*RECORD*
*FIELD* NO
312760
*FIELD* TI
*312760 RIBOSOMAL PROTEIN S4, X-LINKED; RPS4X
SINGLE-COPY ABUNDANT mRNA; SCAR;;
CELL CYCLE GENE 2; CCG2
read more*FIELD* TX
RPS4Y (470000), a Y-linked gene gene in the human, encodes ribosomal
protein S4. A homologous locus on the human X chromosome, RPS4X, lies
close to the X-inactivation center (314670) but fails to undergo X
inactivation. Genetic mapping utilizing interspecific backcrosses and an
intron probe derived from the mouse Rps4 gene demonstrated that Rps4
maps close to the Phka locus on the mouse X chromosome and in the
vicinity of the X-inactivation center (Hamvas et al., 1991, 1992).
Lafreniere et al. (1993) studied a 2.6-Mb contig of YACs that completely
covered the region of the X-inactivation center and physically linked
RPS4X, PHKA1, XIST, and DXS128E (an expressed DNA segment of unknown
function), as well as a laminin receptor pseudogene (LAMRP4; see
150370). The order of genes was shown to be
cen--RPS4X--PHKA1--XIST--DXS128E--tel. The transcriptional orientation
of the RPS4X gene was cen--5-prime--3-prime--qter.
Fisher et al. (1990) demonstrated that the RPS4Y and RPS4X proteins
differ at 19 of 263 amino acids. Both genes are widely transcribed in
human tissues, suggesting that the ribosomes of human males and females
are structurally distinct. By transcription analysis, Fisher et al.
(1990) found that 'unlike most genes on the X chromosome, RPS4X is not
dosage compensated.' RPS4X was the first gene on the long arm of the X
chromosome known to escape X inactivation. On the Y chromosome, RPS4Y
maps to a 90-kb segment that has been implicated in Turner syndrome. XY
gonadal dysgenesis patients with somatic features of the Turner syndrome
have been found to have deletion of this portion of Yp. The Turner
phenotype, or at least its extragonadal component, is probably the
result of the presence of 1 rather than 2 copies of a gene or genes
common to the X and Y chromosomes ('haploinsufficiency'). Kenmochi et
al. (1998) confirmed the mapping assignment of the RPS4X gene to Xq.
Zinn et al. (1991) found that in the mouse the Rps4 gene is indeed
subject to X inactivation. This finding may explain why the phenotypic
consequences of X monosomy are less severe in mice than in humans; the
X0 mouse is a fertile female.
Watanabe et al. (1993) demonstrated that the RPS4Y and RPS4X ribosomal
proteins are interchangeable and provide an essential function: either
protein rescued a mutant hamster cell line that was otherwise incapable
of growth at modestly elevated temperatures. These findings are
consistent with the hypothesis that RPS4 deficiency has a role in Turner
syndrome.
Geerkens et al. (1996) concluded that haploinsufficiency of RPS4X cannot
be the cause of Turner syndrome because patients with 46,Xi(Xq)
karyotype, i.e., isochromosome Xq, cannot be differentiated
phenotypically from 45,X Turner syndrome patients but carry 3 copies of
the RPS4X gene. In 4 patients with typical manifestations and a
nonmosaic chromosome complement of isochromosome Xq, the authors found
significantly increased RPS4X mRNA levels.
Wiles et al. (1988) constructed a cDNA library from a mouse-human
somatic cell hybrid containing as its only human contribution an X-6
translocation chromosome. From among clones that hybridized most
strongly with DNA derived from the hybrid than with a 'mouse only' cell
line, they found 1 devoid of repeats. This clone, called SCR10,
identified a 1-kb mRNA transcribed from the human X chromosome and
mapping to the region Xq13-q13.3 or Xq21.3-q22. It represented an
abundantly and ubiquitously expressed gene. A full-length or nearly
full-length clone of SCR10, SCAR, was isolated and sequenced; the
conceptual translation of this sequence indicated a basic protein of
27.5 kD. Sequences homologous to SCAR were detected in primates,
rodents, avians, and Xenopus.
Watanabe et al. (1991) demonstrated that CCG2, the human gene that
complements the temperature-sensitive cell cycle mutant tsBN63, is
identical to the SCAR/RPS4X gene.
Omoe and Endo (1996) compared sequences of the X- and Y-linked RPS4
genes from several mammals and showed that these 2 loci diverged prior
to the radiation of the placental mammals. Furthermore, the Y-linked
homolog is absent in many species, members of which can show the
monosomy X phenotype (Turner syndrome in humans). From this the authors
concluded that, rather than single RPS4 haploinsufficiency, there may be
other genes that contribute to abnormal phenotypes of monosomy X.
*FIELD* RF
1. Fisher, E. M. C.; Beer-Romero, P.; Brown, L. G.; Ridley, A.; McNeil,
J. A.; Lawrence, J. B.; Willard, H. F.; Bieber, F. R.; Page, D. C.
: Homologous ribosomal protein genes on the human X and Y chromosomes:
escape from X inactivation and possible implications for Turner syndrome. Cell 63:
1205-1218, 1990.
2. Geerkens, C.; Just, W.; Held, K. R.; Vogel, W.: Ullrich-Turner
syndrome is not caused by haploinsufficiency of RPS4X. Hum. Genet. 97:
39-44, 1996.
3. Hamvas, R. M.; Brown, S. D.; Keer, J. T.; Fisher, E. M.; Romero,
P.; Zinn, A.; Page, D.: The mapping of the locus Rps4 to the X-inactivation
region in the mouse. (Abstract) Cytogenet. Cell Genet. 58: 2065-2066,
1991.
4. Hamvas, R. M. J.; Zinn, A.; Keer, J. T.; Fisher, E. M. C.; Beer-Romero,
P.; Brown, S. D. M.; Page, D. C.: Rps4 maps near the inactivation
center on the mouse X chromosome. Genomics 12: 363-367, 1992.
5. Kenmochi, N.; Kawaguchi, T.; Rozen, S.; Davis, E.; Goodman, N.;
Hudson, T. J.; Tanaka, T.; Page, D. C.: A map of 75 human ribosomal
protein genes. Genome Res. 8: 509-523, 1998.
6. Lafreniere, R. G.; Brown, C. J.; Rider, S.; Chelly, J.; Taillon-Miller,
P.; Chinault, A. C.; Monaco, A. P.; Willard, H. F.: 2.6 Mb YAC contig
of the human X inactivation center region in Xq13: physical linkage
of the RPS4X, PHKA1, XIST and DXS128E genes. Hum. Molec. Genet. 2:
1105-1115, 1993.
7. Omoe, K.; Endo, A.: Relationship between the monosomy X phenotype
and Y-linked ribosomal protein S4 (Rps4) in several species of mammals:
a molecular evolutionary analysis of Rps4 homologs. Genomics 31:
44-50, 1996.
8. Watanabe, M.; Furuno, N.; Goebl, M.; Go, M.; Miyauchi, K.; Sekiguchi,
T.; Basilico, C.; Nishimito, T.: Molecular cloning of the human gene,
CCG2, that complements the BHK-derived temperature-sensitive cell
cycle mutant tsBN63: identity of CCG2 with the human X chromosomal
SCAR/RPS4X gene. J. Cell Sci. 100: 35-43, 1991.
9. Watanabe, M.; Zinn, A. R.; Page, D. C.; Nishimoto, T.: Functional
equivalence of human X- and Y-encoded isoforms of ribosomal protein
S4 consistent with a role in Turner syndrome. Nature Genet. 4: 268-271,
1993.
10. Wiles, M. V.; Alexander, C. M.; Goodfellow, P. N.: Isolation
of an abundantly expressed sequence from the human X chromosome by
differential screening. Somat. Cell Molec. Genet. 14: 31-39, 1988.
11. Zinn, A. R.; Bressler, S. L.; Beer-Romero, P.; Adler, D. A.; Chapman,
V. M.; Page, D. C.; Disteche, C. M.: Inactivation of the Rps4 gene
on the mouse X chromosome. Genomics 11: 1097-1101, 1991. Note: Erratum:
Genomics 13: 915 only, 1992.
*FIELD* CN
Patti M. Sherman - updated: 3/11/1999
Alan F. Scott - updated: 4/8/1996
*FIELD* CD
Victor A. McKusick: 9/4/1991
*FIELD* ED
carol: 05/10/2012
carol: 4/7/1999
dkim: 12/15/1998
carol: 9/4/1998
mark: 11/18/1996
terry: 4/17/1996
mark: 4/8/1996
terry: 4/8/1996
mark: 4/8/1996
mark: 1/14/1996
mimadm: 2/28/1994
carol: 9/23/1993
carol: 9/21/1993
carol: 9/20/1993
carol: 5/25/1993
carol: 4/5/1993
*RECORD*
*FIELD* NO
312760
*FIELD* TI
*312760 RIBOSOMAL PROTEIN S4, X-LINKED; RPS4X
SINGLE-COPY ABUNDANT mRNA; SCAR;;
CELL CYCLE GENE 2; CCG2
read more*FIELD* TX
RPS4Y (470000), a Y-linked gene gene in the human, encodes ribosomal
protein S4. A homologous locus on the human X chromosome, RPS4X, lies
close to the X-inactivation center (314670) but fails to undergo X
inactivation. Genetic mapping utilizing interspecific backcrosses and an
intron probe derived from the mouse Rps4 gene demonstrated that Rps4
maps close to the Phka locus on the mouse X chromosome and in the
vicinity of the X-inactivation center (Hamvas et al., 1991, 1992).
Lafreniere et al. (1993) studied a 2.6-Mb contig of YACs that completely
covered the region of the X-inactivation center and physically linked
RPS4X, PHKA1, XIST, and DXS128E (an expressed DNA segment of unknown
function), as well as a laminin receptor pseudogene (LAMRP4; see
150370). The order of genes was shown to be
cen--RPS4X--PHKA1--XIST--DXS128E--tel. The transcriptional orientation
of the RPS4X gene was cen--5-prime--3-prime--qter.
Fisher et al. (1990) demonstrated that the RPS4Y and RPS4X proteins
differ at 19 of 263 amino acids. Both genes are widely transcribed in
human tissues, suggesting that the ribosomes of human males and females
are structurally distinct. By transcription analysis, Fisher et al.
(1990) found that 'unlike most genes on the X chromosome, RPS4X is not
dosage compensated.' RPS4X was the first gene on the long arm of the X
chromosome known to escape X inactivation. On the Y chromosome, RPS4Y
maps to a 90-kb segment that has been implicated in Turner syndrome. XY
gonadal dysgenesis patients with somatic features of the Turner syndrome
have been found to have deletion of this portion of Yp. The Turner
phenotype, or at least its extragonadal component, is probably the
result of the presence of 1 rather than 2 copies of a gene or genes
common to the X and Y chromosomes ('haploinsufficiency'). Kenmochi et
al. (1998) confirmed the mapping assignment of the RPS4X gene to Xq.
Zinn et al. (1991) found that in the mouse the Rps4 gene is indeed
subject to X inactivation. This finding may explain why the phenotypic
consequences of X monosomy are less severe in mice than in humans; the
X0 mouse is a fertile female.
Watanabe et al. (1993) demonstrated that the RPS4Y and RPS4X ribosomal
proteins are interchangeable and provide an essential function: either
protein rescued a mutant hamster cell line that was otherwise incapable
of growth at modestly elevated temperatures. These findings are
consistent with the hypothesis that RPS4 deficiency has a role in Turner
syndrome.
Geerkens et al. (1996) concluded that haploinsufficiency of RPS4X cannot
be the cause of Turner syndrome because patients with 46,Xi(Xq)
karyotype, i.e., isochromosome Xq, cannot be differentiated
phenotypically from 45,X Turner syndrome patients but carry 3 copies of
the RPS4X gene. In 4 patients with typical manifestations and a
nonmosaic chromosome complement of isochromosome Xq, the authors found
significantly increased RPS4X mRNA levels.
Wiles et al. (1988) constructed a cDNA library from a mouse-human
somatic cell hybrid containing as its only human contribution an X-6
translocation chromosome. From among clones that hybridized most
strongly with DNA derived from the hybrid than with a 'mouse only' cell
line, they found 1 devoid of repeats. This clone, called SCR10,
identified a 1-kb mRNA transcribed from the human X chromosome and
mapping to the region Xq13-q13.3 or Xq21.3-q22. It represented an
abundantly and ubiquitously expressed gene. A full-length or nearly
full-length clone of SCR10, SCAR, was isolated and sequenced; the
conceptual translation of this sequence indicated a basic protein of
27.5 kD. Sequences homologous to SCAR were detected in primates,
rodents, avians, and Xenopus.
Watanabe et al. (1991) demonstrated that CCG2, the human gene that
complements the temperature-sensitive cell cycle mutant tsBN63, is
identical to the SCAR/RPS4X gene.
Omoe and Endo (1996) compared sequences of the X- and Y-linked RPS4
genes from several mammals and showed that these 2 loci diverged prior
to the radiation of the placental mammals. Furthermore, the Y-linked
homolog is absent in many species, members of which can show the
monosomy X phenotype (Turner syndrome in humans). From this the authors
concluded that, rather than single RPS4 haploinsufficiency, there may be
other genes that contribute to abnormal phenotypes of monosomy X.
*FIELD* RF
1. Fisher, E. M. C.; Beer-Romero, P.; Brown, L. G.; Ridley, A.; McNeil,
J. A.; Lawrence, J. B.; Willard, H. F.; Bieber, F. R.; Page, D. C.
: Homologous ribosomal protein genes on the human X and Y chromosomes:
escape from X inactivation and possible implications for Turner syndrome. Cell 63:
1205-1218, 1990.
2. Geerkens, C.; Just, W.; Held, K. R.; Vogel, W.: Ullrich-Turner
syndrome is not caused by haploinsufficiency of RPS4X. Hum. Genet. 97:
39-44, 1996.
3. Hamvas, R. M.; Brown, S. D.; Keer, J. T.; Fisher, E. M.; Romero,
P.; Zinn, A.; Page, D.: The mapping of the locus Rps4 to the X-inactivation
region in the mouse. (Abstract) Cytogenet. Cell Genet. 58: 2065-2066,
1991.
4. Hamvas, R. M. J.; Zinn, A.; Keer, J. T.; Fisher, E. M. C.; Beer-Romero,
P.; Brown, S. D. M.; Page, D. C.: Rps4 maps near the inactivation
center on the mouse X chromosome. Genomics 12: 363-367, 1992.
5. Kenmochi, N.; Kawaguchi, T.; Rozen, S.; Davis, E.; Goodman, N.;
Hudson, T. J.; Tanaka, T.; Page, D. C.: A map of 75 human ribosomal
protein genes. Genome Res. 8: 509-523, 1998.
6. Lafreniere, R. G.; Brown, C. J.; Rider, S.; Chelly, J.; Taillon-Miller,
P.; Chinault, A. C.; Monaco, A. P.; Willard, H. F.: 2.6 Mb YAC contig
of the human X inactivation center region in Xq13: physical linkage
of the RPS4X, PHKA1, XIST and DXS128E genes. Hum. Molec. Genet. 2:
1105-1115, 1993.
7. Omoe, K.; Endo, A.: Relationship between the monosomy X phenotype
and Y-linked ribosomal protein S4 (Rps4) in several species of mammals:
a molecular evolutionary analysis of Rps4 homologs. Genomics 31:
44-50, 1996.
8. Watanabe, M.; Furuno, N.; Goebl, M.; Go, M.; Miyauchi, K.; Sekiguchi,
T.; Basilico, C.; Nishimito, T.: Molecular cloning of the human gene,
CCG2, that complements the BHK-derived temperature-sensitive cell
cycle mutant tsBN63: identity of CCG2 with the human X chromosomal
SCAR/RPS4X gene. J. Cell Sci. 100: 35-43, 1991.
9. Watanabe, M.; Zinn, A. R.; Page, D. C.; Nishimoto, T.: Functional
equivalence of human X- and Y-encoded isoforms of ribosomal protein
S4 consistent with a role in Turner syndrome. Nature Genet. 4: 268-271,
1993.
10. Wiles, M. V.; Alexander, C. M.; Goodfellow, P. N.: Isolation
of an abundantly expressed sequence from the human X chromosome by
differential screening. Somat. Cell Molec. Genet. 14: 31-39, 1988.
11. Zinn, A. R.; Bressler, S. L.; Beer-Romero, P.; Adler, D. A.; Chapman,
V. M.; Page, D. C.; Disteche, C. M.: Inactivation of the Rps4 gene
on the mouse X chromosome. Genomics 11: 1097-1101, 1991. Note: Erratum:
Genomics 13: 915 only, 1992.
*FIELD* CN
Patti M. Sherman - updated: 3/11/1999
Alan F. Scott - updated: 4/8/1996
*FIELD* CD
Victor A. McKusick: 9/4/1991
*FIELD* ED
carol: 05/10/2012
carol: 4/7/1999
dkim: 12/15/1998
carol: 9/4/1998
mark: 11/18/1996
terry: 4/17/1996
mark: 4/8/1996
terry: 4/8/1996
mark: 4/8/1996
mark: 1/14/1996
mimadm: 2/28/1994
carol: 9/23/1993
carol: 9/21/1993
carol: 9/20/1993
carol: 5/25/1993
carol: 4/5/1993