RBCC Red Blood Cell Collection

SH3BGRL [Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
SH3 domain-binding glutamic acid-rich-like protein
Note: presumably soluble (membrane word is not in UniProt keywords or features)

UniProt O75368
ID SH3L1_HUMAN Reviewed; 114 AA.
AC O75368; Q3SYL1; Q5JT50; Q6FIE8; Q9H0N8;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1998, sequence version 1.
DT 22-JAN-2014, entry version 117.
DE RecName: Full=SH3 domain-binding glutamic acid-rich-like protein;
GN Name=SH3BGRL;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=9642120; DOI=10.1006/bbrc.1998.8763;
RA Egeo A., Mazzocco M., Arrigo P., Vidal-Taboada J.M., Oliva R.,
RA Pirola B., Giglio S., Rasore-Quartino A., Scartezzini P.;
RT "Identification and characterization of a new human gene encoding a
RT small protein with high homology to the proline-rich region of the
RT SH3BGR gene.";
RL Biochem. Biophys. Res. Commun. 247:302-306(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=11230166; DOI=10.1101/gr.GR1547R;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H.,
RA Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N.,
RA Mewes H.-W., Ottenwaelder B., Obermaier B., Tampe J., Heubner D.,
RA Wambutt R., Korn B., Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and
RT analysis of 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A.,
RA Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G.,
RA Jones M.C., Hurles M.E., Andrews T.D., Scott C.E., Searle S.,
RA Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R.,
RA Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L.,
RA Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A.,
RA Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S.,
RA Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R.,
RA Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M.,
RA Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N.,
RA Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D.,
RA Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W.,
RA Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C.,
RA Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C.,
RA Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J.,
RA Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S.,
RA Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I.,
RA Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L.,
RA Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P.,
RA Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S.,
RA Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A.,
RA Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J.,
RA Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J.,
RA Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S.,
RA de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z.,
RA Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C.,
RA Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W.,
RA Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T.,
RA Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I.,
RA Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N.,
RA Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J.,
RA Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E.,
RA Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S.,
RA Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T.,
RA Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S.,
RA Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L.,
RA Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A.,
RA Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L.,
RA Williams G., Williams L., Williamson A., Williamson H., Wilming L.,
RA Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H.,
RA Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A.,
RA Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A.,
RA Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T.,
RA Gibbs R.A., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow, and PNS;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 2-114.
RX PubMed=16080146; DOI=10.1002/prot.20523;
RA Yin L., Xiang Y., Zhu D.Y., Yan N., Huang R.H., Zhang Y., Wang D.C.;
RT "Crystal structure of human SH3BGRL protein: the first structure of
RT the human SH3BGR family representing a novel class of thioredoxin fold
RT proteins.";
RL Proteins 61:213-216(2005).
RN [8]
RP STRUCTURE BY NMR OF 1-108.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the SH3 domain-binding glutamic acid-rich-like
RT protein.";
RL Submitted (APR-2005) to the PDB data bank.
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the SH3BGR family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF042081; AAC27445.1; -; mRNA.
DR EMBL; AL136718; CAB66652.1; -; mRNA.
DR EMBL; CR533478; CAG38509.1; -; mRNA.
DR EMBL; AL357115; CAI39436.1; -; Genomic_DNA.
DR EMBL; BC016709; AAH16709.1; -; mRNA.
DR EMBL; BC103762; AAI03763.1; -; mRNA.
DR PIR; JE0178; JE0178.
DR RefSeq; NP_003013.1; NM_003022.2.
DR UniGene; Hs.108029; -.
DR PDB; 1U6T; X-ray; 1.90 A; A=2-114.
DR PDB; 1WRY; NMR; -; A=1-108.
DR PDBsum; 1U6T; -.
DR PDBsum; 1WRY; -.
DR ProteinModelPortal; O75368; -.
DR SMR; O75368; 2-114.
DR IntAct; O75368; 2.
DR MINT; MINT-1562247; -.
DR STRING; 9606.ENSP00000362308; -.
DR PhosphoSite; O75368; -.
DR DOSAC-COBS-2DPAGE; O75368; -.
DR OGP; O75368; -.
DR REPRODUCTION-2DPAGE; O75368; -.
DR SWISS-2DPAGE; O75368; -.
DR PaxDb; O75368; -.
DR PeptideAtlas; O75368; -.
DR PRIDE; O75368; -.
DR Ensembl; ENST00000373212; ENSP00000362308; ENSG00000131171.
DR GeneID; 6451; -.
DR KEGG; hsa:6451; -.
DR UCSC; uc004eef.3; human.
DR CTD; 6451; -.
DR GeneCards; GC0XP080457; -.
DR HGNC; HGNC:10823; SH3BGRL.
DR MIM; 300190; gene.
DR neXtProt; NX_O75368; -.
DR PharmGKB; PA35731; -.
DR eggNOG; NOG287853; -.
DR HOGENOM; HOG000095212; -.
DR HOVERGEN; HBG054781; -.
DR InParanoid; O75368; -.
DR OMA; TAIKKQQ; -.
DR OrthoDB; EOG7GTT63; -.
DR PhylomeDB; O75368; -.
DR ChiTaRS; SH3BGRL; human.
DR EvolutionaryTrace; O75368; -.
DR GeneWiki; SH3BGRL; -.
DR GenomeRNAi; 6451; -.
DR NextBio; 25075; -.
DR PRO; PR:O75368; -.
DR ArrayExpress; O75368; -.
DR Bgee; O75368; -.
DR CleanEx; HS_SH3BGRL; -.
DR Genevestigator; O75368; -.
DR GO; GO:0005737; C:cytoplasm; TAS:UniProtKB.
DR GO; GO:0005634; C:nucleus; TAS:UniProtKB.
DR GO; GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
DR Gene3D; 3.40.30.10; -; 1.
DR InterPro; IPR006993; Glut_rich_SH3-bd.
DR InterPro; IPR012336; Thioredoxin-like_fold.
DR PANTHER; PTHR12232; PTHR12232; 1.
DR Pfam; PF04908; SH3BGR; 1.
DR PIRSF; PIRSF008142; SH3-bind_E-rich_L; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Reference proteome; SH3-binding.
FT CHAIN 1 114 SH3 domain-binding glutamic acid-rich-
FT like protein.
FT /FTId=PRO_0000220745.
FT MOTIF 61 67 SH3-binding (Potential).
FT CONFLICT 35 35 E -> K (in Ref. 2 and 3).
FT CONFLICT 113 113 Q -> R (in Ref. 2 and 3).
FT STRAND 3 7
FT HELIX 14 29
FT STRAND 34 38
FT HELIX 43 52
FT HELIX 55 57
FT STRAND 60 63
FT STRAND 68 71
FT STRAND 74 78
FT HELIX 79 87
FT HELIX 91 95
FT HELIX 104 114
SQ SEQUENCE 114 AA; 12774 MW; D72AC2A095F1AA8B CRC64;
MVIRVYIASS SGSTAIKKKQ QDVLGFLEAN KIGFEEKDIA ANEENRKWMR ENVPENSRPA
TGYPLPPQIF NESQYRGDYD AFFEARENNA VYAFLGLTAP PGSKEAEVQA KQQA
//
read less

MIM 300190
*RECORD*
*FIELD* NO
300190
*FIELD* TI
*300190 SH3-BINDING DOMAIN AND GLUTAMIC ACID-RICH PROTEIN-LIKE PROTEIN; SH3BGRL
;;SH3BGR-LIKE PROTEIN
read more*FIELD* TX

CLONING

Proline-rich peptide sequences have been shown to play important roles
in protein-protein interactions that occur in signal transduction
pathways. Specific proline-rich consensus sequences that bind SH3
domains have been identified (e.g., see SH3BGR; 602230). Egeo et al.
(1998) screened an EST database with the SH3BGR nucleotide sequence and
identified 3 overlapping human infant brain cDNA clones encoding SH3BGRL
(SH3BGR-like). The predicted 114-amino acid SH3BGRL protein has a
potential cell attachment sequence. A proline-rich sequence containing
an SH3-binding motif is present in the middle of the protein. The
SH3BGRL protein shares 60% identity with the proline-rich central region
of SH3BGR. Northern blot analysis detected a single SH3BGRL transcript
of approximately 1.9 kb that was present in all tissues examined. Egeo
et al. (1998) identified a mouse cDNA encoding a deduced protein that is
95% identical to human SH3BGRL.

MAPPING

Egeo et al. (1998) assigned the SH3BGRL gene to Xq13.3 by FISH.

*FIELD* RF
1. Egeo, A.; Mazzocco, M.; Arrigo, P.; Vidal-Taboada, J. M.; Oliva,
R.; Pirola, B.; Giglio, S.; Rasore-Quartino, A.; Scartezzini, P.:
Identification and characterization of a new human gene encoding a
small protein with high homology to the proline-rich region of the
SH3BGR gene. Biochem. Biophys. Res. Commun. 247: 302-306, 1998.

*FIELD* CD
Barbara J. Biery: 5/18/1999

*FIELD* ED
carol: 01/10/2014
psherman: 1/27/2000
psherman: 5/18/1999

read less