RBCC

Full text data of SLITRK2

SLITRK2 (CXorf2, KIAA1854, SLITL1) [Confidence: low (only semi-automatic identification from reviews)]
SLIT and NTRK-like protein 2; Flags: Precursor

UniProt Q9H156
ID SLIK2_HUMAN Reviewed; 845 AA.
AC Q9H156; A8K117; Q2KHN3; Q5JXB1; Q8NBC7; Q96JH3;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-MAR-2001, sequence version 1.
DT 22-JAN-2014, entry version 108.
DE RecName: Full=SLIT and NTRK-like protein 2;
DE Flags: Precursor;
GN Name=SLITRK2; Synonyms=CXorf2, KIAA1854, SLITL1;
GN ORFNames=UNQ9197/PRO34756;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
RA Redolfi E., Susani L., Mumm S., Stephan A., Reinbold R.A., Labella T.,
RA Trent J.M., Vezzoni P., Zucchi T.;
RT "The structural characterization of the new gene SLITL1 reveals the
RT presence of an other novel gene embedded within SLITL1 first intron.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A.,
RA Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G.,
RA Jones M.C., Hurles M.E., Andrews T.D., Scott C.E., Searle S.,
RA Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R.,
RA Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L.,
RA Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A.,
RA Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S.,
RA Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R.,
RA Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M.,
RA Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N.,
RA Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D.,
RA Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W.,
RA Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C.,
RA Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C.,
RA Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J.,
RA Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S.,
RA Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I.,
RA Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L.,
RA Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P.,
RA Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S.,
RA Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A.,
RA Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J.,
RA Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J.,
RA Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S.,
RA de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z.,
RA Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C.,
RA Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W.,
RA Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T.,
RA Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I.,
RA Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N.,
RA Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J.,
RA Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E.,
RA Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S.,
RA Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T.,
RA Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S.,
RA Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L.,
RA Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A.,
RA Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L.,
RA Williams G., Williams L., Williamson A., Williamson H., Wilming L.,
RA Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H.,
RA Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A.,
RA Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A.,
RA Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T.,
RA Gibbs R.A., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-566.
RC TISSUE=Brain;
RX PubMed=11347906; DOI=10.1093/dnares/8.2.85;
RA Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XX.
RT The complete sequences of 100 new cDNA clones from brain which code
RT for large proteins in vitro.";
RL DNA Res. 8:85-95(2001).
RN [7]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RC TISSUE=Brain, and Brain tumor;
RX PubMed=14557068; DOI=10.1016/S0378-1119(03)00715-7;
RA Aruga J., Yokota N., Mikoshiba K.;
RT "Human SLITRK family genes: genomic organization and expression
RT profiling in normal brain and brain tumor tissue.";
RL Gene 315:87-94(2003).
CC -!- FUNCTION: Suppresses neurite outgrowth (By similarity).
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane
CC protein (Potential).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9H156-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H156-2; Sequence=VSP_050706, VSP_050707;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in the cerebral cortex
CC of the brain but also at low levels in the spinal cord and
CC medulla. Also expressed in some astrocytic brain tumors such as
CC astrocytomas, oligodendrogliomas, glioblastomas, gangliogliomas
CC and primitive neuroectodermal tumors.
CC -!- SIMILARITY: Belongs to the SLITRK family.
CC -!- SIMILARITY: Contains 12 LRR (leucine-rich) repeats.
CC -!- SIMILARITY: Contains 2 LRRCT domains.
CC -!- SIMILARITY: Contains 1 LRRNT domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB47483.1; Type=Erroneous initiation;
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DR EMBL; Y19205; CAC80724.1; -; Genomic_DNA.
DR EMBL; AY358828; AAQ89187.1; -; mRNA.
DR EMBL; AK091015; BAC03566.1; -; mRNA.
DR EMBL; AK289732; BAF82421.1; -; mRNA.
DR EMBL; AL109653; CAI41645.1; -; Genomic_DNA.
DR EMBL; AL109653; CAC18888.1; -; Genomic_DNA.
DR EMBL; BC113011; AAI13012.1; -; mRNA.
DR EMBL; BC113012; AAI13013.1; -; mRNA.
DR EMBL; AB058757; BAB47483.1; ALT_INIT; mRNA.
DR RefSeq; NP_001137475.1; NM_001144003.2.
DR RefSeq; NP_001137476.1; NM_001144004.2.
DR RefSeq; NP_001137477.1; NM_001144005.2.
DR RefSeq; NP_001137478.1; NM_001144006.2.
DR RefSeq; NP_001137480.1; NM_001144008.2.
DR RefSeq; NP_001137481.1; NM_001144009.2.
DR RefSeq; NP_001137482.1; NM_001144010.2.
DR RefSeq; NP_115928.1; NM_032539.4.
DR RefSeq; XP_005262399.1; XM_005262342.1.
DR RefSeq; XP_005262400.1; XM_005262343.1.
DR RefSeq; XP_005262401.1; XM_005262344.1.
DR RefSeq; XP_005262402.1; XM_005262345.1.
DR RefSeq; XP_005262403.1; XM_005262346.1.
DR RefSeq; XP_005278257.1; XM_005278200.1.
DR RefSeq; XP_005278258.1; XM_005278201.1.
DR RefSeq; XP_005278259.1; XM_005278202.1.
DR RefSeq; XP_005278260.1; XM_005278203.1.
DR RefSeq; XP_005278261.1; XM_005278204.1.
DR UniGene; Hs.320368; -.
DR ProteinModelPortal; Q9H156; -.
DR SMR; Q9H156; 50-610.
DR STRING; 9606.ENSP00000359521; -.
DR PhosphoSite; Q9H156; -.
DR DMDM; 46397026; -.
DR PaxDb; Q9H156; -.
DR PRIDE; Q9H156; -.
DR Ensembl; ENST00000370490; ENSP00000359521; ENSG00000185985.
DR Ensembl; ENST00000413937; ENSP00000407347; ENSG00000185985.
DR Ensembl; ENST00000428560; ENSP00000412010; ENSG00000185985.
DR Ensembl; ENST00000434188; ENSP00000397015; ENSG00000185985.
DR Ensembl; ENST00000447897; ENSP00000411681; ENSG00000185985.
DR Ensembl; ENST00000596854; ENSP00000472699; ENSG00000268737.
DR Ensembl; ENST00000597957; ENSP00000468887; ENSG00000268737.
DR Ensembl; ENST00000597981; ENSP00000469687; ENSG00000268737.
DR Ensembl; ENST00000600096; ENSP00000471447; ENSG00000268737.
DR Ensembl; ENST00000601272; ENSP00000472603; ENSG00000268737.
DR GeneID; 84631; -.
DR KEGG; hsa:84631; -.
DR UCSC; uc004fcd.3; human.
DR CTD; 84631; -.
DR GeneCards; GC0XP144900; -.
DR H-InvDB; HIX0017096; -.
DR H-InvDB; HIX0176721; -.
DR HGNC; HGNC:13449; SLITRK2.
DR HPA; HPA051847; -.
DR MIM; 300561; gene.
DR neXtProt; NX_Q9H156; -.
DR PharmGKB; PA134968225; -.
DR eggNOG; NOG147171; -.
DR HOGENOM; HOG000290667; -.
DR HOVERGEN; HBG056407; -.
DR InParanoid; Q9H156; -.
DR OMA; LQADYNY; -.
DR PhylomeDB; Q9H156; -.
DR ChiTaRS; SLITRK2; human.
DR GeneWiki; SLITRK2; -.
DR GenomeRNAi; 84631; -.
DR NextBio; 74551; -.
DR PRO; PR:Q9H156; -.
DR ArrayExpress; Q9H156; -.
DR Bgee; Q9H156; -.
DR CleanEx; HS_SLITRK2; -.
DR Genevestigator; Q9H156; -.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0007409; P:axonogenesis; IEA:Ensembl.
DR InterPro; IPR000483; Cys-rich_flank_reg_C.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR000372; LRR-contain_N.
DR Pfam; PF00560; LRR_1; 2.
DR SMART; SM00369; LRR_TYP; 3.
DR SMART; SM00082; LRRCT; 2.
DR SMART; SM00013; LRRNT; 2.
DR PROSITE; PS51450; LRR; 12.
PE 2: Evidence at transcript level;
KW Alternative splicing; Complete proteome; Glycoprotein;
KW Leucine-rich repeat; Membrane; Phosphoprotein; Polymorphism;
KW Reference proteome; Repeat; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1 21 Potential.
FT CHAIN 22 845 SLIT and NTRK-like protein 2.
FT /FTId=PRO_0000032675.
FT TOPO_DOM 22 621 Extracellular (Potential).
FT TRANSMEM 622 642 Helical; (Potential).
FT TOPO_DOM 643 845 Cytoplasmic (Potential).
FT REPEAT 63 84 LRR 1.
FT REPEAT 87 108 LRR 2.
FT REPEAT 111 132 LRR 3.
FT REPEAT 135 156 LRR 4.
FT REPEAT 159 180 LRR 5.
FT REPEAT 182 203 LRR 6.
FT DOMAIN 216 265 LRRCT 1.
FT DOMAIN 331 373 LRRNT.
FT REPEAT 376 397 LRR 7.
FT REPEAT 400 421 LRR 8.
FT REPEAT 424 445 LRR 9.
FT REPEAT 448 469 LRR 10.
FT REPEAT 472 493 LRR 11.
FT REPEAT 495 516 LRR 12.
FT DOMAIN 529 580 LRRCT 2.
FT MOD_RES 756 756 Phosphotyrosine (By similarity).
FT CARBOHYD 84 84 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 421 421 N-linked (GlcNAc...) (Potential).
FT VAR_SEQ 719 733 SYSNLEEKKEEPATP -> KTSLENIWRPCLHKK (in
FT isoform 2).
FT /FTId=VSP_050706.
FT VAR_SEQ 734 845 Missing (in isoform 2).
FT /FTId=VSP_050707.
FT VARIANT 601 601 S -> P (in dbSNP:rs2295336).
FT /FTId=VAR_027756.
FT CONFLICT 721 721 S -> G (in Ref. 3; BAC03566).
SQ SEQUENCE 845 AA; 95404 MW; 20E35DBD1584F9A4 CRC64;
MLSGVWFLSV LTVAGILQTE SRKTAKDICK IRCLCEEKEN VLNINCENKG FTTVSLLQPP
QYRIYQLFLN GNLLTRLYPN EFVNYSNAVT LHLGNNGLQE IRTGAFSGLK TLKRLHLNNN
KLEILREDTF LGLESLEYLQ ADYNYISAIE AGAFSKLNKL KVLILNDNLL LSLPSNVFRF
VLLTHLDLRG NRLKVMPFAG VLEHIGGIME IQLEENPWNC TCDLLPLKAW LDTITVFVGE
IVCETPFRLH GKDVTQLTRQ DLCPRKSASD SSQRGSHADT HVQRLSPTMN PALNPTRAPK
ASRPPKMRNR PTPRVTVSKD RQSFGPIMVY QTKSPVPLTC PSSCVCTSQS SDNGLNVNCQ
ERKFTNISDL QPKPTSPKKL YLTGNYLQTV YKNDLLEYSS LDLLHLGNNR IAVIQEGAFT
NLTSLRRLYL NGNYLEVLYP SMFDGLQSLQ YLYLEYNVIK EIKPLTFDAL INLQLLFLNN
NLLRSLPDNI FGGTALTRLN LRNNHFSHLP VKGVLDQLPA FIQIDLQENP WDCTCDIMGL
KDWTEHANSP VIINEVTCES PAKHAGEILK FLGREAICPD SPNLSDGTVL SMNHNTDTPR
SLSVSPSSYP ELHTEVPLSV LILGLLVVFI LSVCFGAGLF VFVLKRRKGV PSVPRNTNNL
DVSSFQLQYG SYNTETHDKT DGHVYNYIPP PVGQMCQNPI YMQKEGDPVA YYRNLQEFSY
SNLEEKKEEP ATPAYTISAT ELLEKQATPR EPELLYQNIA ERVKELPSAG LVHYNFCTLP
KRQFAPSYES RRQNQDRINK TVLYGTPRKC FVGQSKPNHP LLQAKPQSEP DYLEVLEKQT
AISQL
//

MIM 300561
*RECORD*
*FIELD* NO
300561
*FIELD* TI
*300561 SLIT- AND NTRK-LIKE FAMILY, MEMBER 2; SLITRK2
;;KIAA1854
*FIELD* TX

DESCRIPTION
read more
Members of the SLITRK family, such as SLITRK2, are integral membrane
proteins with 2 N-terminal leucine-rich repeat (LRR) domains similar to
those of SLIT proteins (see SLIT1; 603742). Most SLITRKs, including
SLITRK2, also have C-terminal regions that share homology with
neurotrophin receptors (see NTRK1; 191315). SLITRKs are expressed
predominantly in neural tissues and have neurite-modulating activity
(Aruga et al., 2003).

CLONING

By sequencing clones obtained from a size-fractionated fetal brain cDNA
library, Nagase et al. (2001) cloned SLITRK2, which they designated
KIAA1854. The deduced protein contains 572 amino acids. RT-PCR ELISA
detected intermediate expression in adult and fetal brain, adult spinal
cord, and all specific brain regions examined. Little to no expression
was detected in nonneural tissues.

Aruga and Mikoshiba (2003) cloned several mouse Slitrk cDNAs, including
Slitrk2. The deduced Slitrk2 protein contains an N-terminal signal
peptide, followed by 2 LRR domains, a transmembrane domain, and a
cytoplasmic domain with 4 putative tyrosine phosphorylation sites. Each
LRR domain is flanked by cysteine-rich regions. Northern blot analysis
of several mouse tissues detected high Slitrk2 expression only in brain.
In situ hybridization of developing mouse brain revealed broad Slitrk2
expression, with significant expression in the ventricular layer
(particularly the ventricular side), subventricular zone, cortical
plate, pyramidal layer of hippocampus, subicular neuroepithelium,
thalamus, hypothalamus, and spinal cord. In a rat pheochromocytoma cell
line, Slitrk2 localized to the trans-Golgi network and the cell
periphery.

By database analysis, Aruga et al. (2003) identified human SLITRK2. The
deduced 845-amino acid protein has a calculated molecular mass of 95.4
kD. It shares more than 95% homology with mouse Slitrk2 and has the same
protein structure. Northern blot analysis detected SLITRK2 transcripts
of 5.1 and 4.1 kb mainly in adult and fetal neural tissues. A minor
transcript of about 8.4 kb was also detected in adult cerebral cortex
and fetal whole brain. Highest SLITRK2 expression was in adult cerebral
cortex.

GENE FUNCTION

By overexpression in murine pheochromocytoma and neuroblastoma cell
lines, Aruga and Mikoshiba (2003) found that mouse Slitrk2 suppressed
neurite outgrowth, including cAMP-induced outgrowth. Mutation analysis
localized the inhibitory activity to the C-terminal intracellular domain
of Slitrk2. Slitrk2 lacking this domain induced neurite outgrowth, and
the isolated intracellular domain inhibited neuritogenesis, similar to
intact Slitrk2 protein.

GENE STRUCTURE

Aruga et al. (2003) determined that the SLITRK2 gene contains 2 exons.
Exon 1 is noncoding.

MAPPING

By genomic sequence analysis, Aruga et al. (2003) mapped the SLITRK2
gene to a region of chromosome Xq27 that also contains the SLITRK4 gene
(300562). The intervening sequence between the SLITRK genes is about 2
Mb. Aruga et al. (2003) mapped the mouse Slitrk2 gene to a region of
chromosome XA5 that shares homology of synteny with human chromosome
Xq27.

*FIELD* RF
1. Aruga, J.; Mikoshiba, K.: Identification and characterization
of Slitrk, a novel neuronal transmembrane protein family controlling
neurite outgrowth. Molec. Cell. Neurosci. 24: 117-129, 2003.

2. Aruga, J.; Yokota, N.; Mikoshiba, K.: Human SLITRK family genes:
genomic organization and expression profiling in normal brain and
brain tumor tissue. Gene 315: 87-94, 2003.

3. Nagase, T.; Nakayama, M.; Nakajima, D.; Kikuno, R.; Ohara, O.:
Prediction of the coding sequences of unidentified human genes. XX.
The complete sequences of 100 new cDNA clones from brain which code
for large proteins in vitro. DNA Res. 8: 85-95, 2001.

*FIELD* CD
Patricia A. Hartz: 10/25/2005

*FIELD* ED
mgross: 10/25/2005

RBCC Red Blood Cell Collection

Full text data of SLITRK2