Full text data of SYNGR2
SYNGR2
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Synaptogyrin-2 (Cellugyrin)
Synaptogyrin-2 (Cellugyrin)
UniProt
O43760
ID SNG2_HUMAN Reviewed; 224 AA.
AC O43760; O43762; Q658S7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-1998, sequence version 1.
DT 22-JAN-2014, entry version 120.
DE RecName: Full=Synaptogyrin-2;
DE AltName: Full=Cellugyrin;
GN Name=SYNGR2; ORFNames=UNQ352/PRO615;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=9760194; DOI=10.1007/s004390050795;
RA Kedra D., Pan H.-Q., Seroussi E., Fransson I., Guilbaud C.,
RA Collins J.E., Dunham I., Blennow E., Roe B.A., Piehl F.,
RA Dumanski J.P.;
RT "Characterization of the human synaptogyrin gene family.";
RL Hum. Genet. 103:131-141(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung, and Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 1-18, ACETYLATION AT MET-1, AND MASS SPECTROMETRY.
RC TISSUE=B-cell lymphoma;
RA Bienvenut W.V.;
RL Submitted (FEB-2005) to UniProtKB.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 34-224.
RC TISSUE=Stomach;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC Cytoplasmic vesicle (By similarity).
CC -!- TISSUE SPECIFICITY: Ubiquitous; low expression in brain.
CC -!- SIMILARITY: Belongs to the synaptogyrin family.
CC -!- SIMILARITY: Contains 1 MARVEL domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AJ002308; CAA05325.1; -; mRNA.
DR EMBL; AJ002310; CAA05327.1; -; Genomic_DNA.
DR EMBL; AJ002312; CAA05327.1; JOINED; Genomic_DNA.
DR EMBL; AY358916; AAQ89275.1; -; mRNA.
DR EMBL; BC000407; AAH00407.1; -; mRNA.
DR EMBL; BC029755; AAH29755.1; -; mRNA.
DR EMBL; AL833010; CAH56291.1; -; mRNA.
DR RefSeq; NP_004701.1; NM_004710.3.
DR UniGene; Hs.464210; -.
DR UniGene; Hs.708616; -.
DR ProteinModelPortal; O43760; -.
DR IntAct; O43760; 1.
DR STRING; 9606.ENSP00000225777; -.
DR PhosphoSite; O43760; -.
DR PaxDb; O43760; -.
DR PRIDE; O43760; -.
DR DNASU; 9144; -.
DR Ensembl; ENST00000225777; ENSP00000225777; ENSG00000108639.
DR Ensembl; ENST00000585591; ENSP00000465678; ENSG00000108639.
DR GeneID; 9144; -.
DR KEGG; hsa:9144; -.
DR UCSC; uc002juu.1; human.
DR CTD; 9144; -.
DR GeneCards; GC17P076164; -.
DR HGNC; HGNC:11499; SYNGR2.
DR HPA; HPA014742; -.
DR MIM; 603926; gene.
DR neXtProt; NX_O43760; -.
DR PharmGKB; PA36281; -.
DR eggNOG; NOG306774; -.
DR HOGENOM; HOG000220816; -.
DR HOVERGEN; HBG000896; -.
DR InParanoid; O43760; -.
DR PhylomeDB; O43760; -.
DR GenomeRNAi; 9144; -.
DR NextBio; 34295; -.
DR PRO; PR:O43760; -.
DR ArrayExpress; O43760; -.
DR Bgee; O43760; -.
DR CleanEx; HS_SYNGR2; -.
DR Genevestigator; O43760; -.
DR GO; GO:0016021; C:integral to membrane; NAS:UniProtKB.
DR GO; GO:0008021; C:synaptic vesicle; IEA:Ensembl.
DR GO; GO:0006605; P:protein targeting; IEA:Ensembl.
DR InterPro; IPR008253; Marvel.
DR InterPro; IPR016579; Synaptogyrin.
DR Pfam; PF01284; MARVEL; 1.
DR PIRSF; PIRSF011282; Synaptogyrin; 1.
DR PROSITE; PS51225; MARVEL; 1.
PE 1: Evidence at protein level;
KW Acetylation; Complete proteome; Cytoplasmic vesicle;
KW Direct protein sequencing; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1 224 Synaptogyrin-2.
FT /FTId=PRO_0000183993.
FT TRANSMEM 26 46 Helical; (Potential).
FT TRANSMEM 73 93 Helical; (Potential).
FT TRANSMEM 105 125 Helical; (Potential).
FT TRANSMEM 147 167 Helical; (Potential).
FT DOMAIN 20 171 MARVEL.
FT MOD_RES 1 1 N-acetylmethionine.
FT CONFLICT 160 162 GVL -> VGW (in Ref. 1; CAA05327).
SQ SEQUENCE 224 AA; 24810 MW; EC92C95CE9E5BD41 CRC64;
MESGAYGAAK AGGSFDLRRF LTQPQVVARA VCLVFALIVF SCIYGEGYSN AHESKQMYCV
FNRNEDACRY GSAIGVLAFL ASAFFLVVDA YFPQISNATD RKYLVIGDLL FSALWTFLWF
VGFCFLTNQW AVTNPKDVLV GADSVRAAIT FSFFSIFSWG VLASLAYQRY KAGVDDFIQN
YVDPTPDPNT AYASYPGASV DNYQQPPFTQ NAETTEGYQP PPVY
//
ID SNG2_HUMAN Reviewed; 224 AA.
AC O43760; O43762; Q658S7;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-1998, sequence version 1.
DT 22-JAN-2014, entry version 120.
DE RecName: Full=Synaptogyrin-2;
DE AltName: Full=Cellugyrin;
GN Name=SYNGR2; ORFNames=UNQ352/PRO615;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=9760194; DOI=10.1007/s004390050795;
RA Kedra D., Pan H.-Q., Seroussi E., Fransson I., Guilbaud C.,
RA Collins J.E., Dunham I., Blennow E., Roe B.A., Piehl F.,
RA Dumanski J.P.;
RT "Characterization of the human synaptogyrin gene family.";
RL Hum. Genet. 103:131-141(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung, and Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 1-18, ACETYLATION AT MET-1, AND MASS SPECTROMETRY.
RC TISSUE=B-cell lymphoma;
RA Bienvenut W.V.;
RL Submitted (FEB-2005) to UniProtKB.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 34-224.
RC TISSUE=Stomach;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC Cytoplasmic vesicle (By similarity).
CC -!- TISSUE SPECIFICITY: Ubiquitous; low expression in brain.
CC -!- SIMILARITY: Belongs to the synaptogyrin family.
CC -!- SIMILARITY: Contains 1 MARVEL domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AJ002308; CAA05325.1; -; mRNA.
DR EMBL; AJ002310; CAA05327.1; -; Genomic_DNA.
DR EMBL; AJ002312; CAA05327.1; JOINED; Genomic_DNA.
DR EMBL; AY358916; AAQ89275.1; -; mRNA.
DR EMBL; BC000407; AAH00407.1; -; mRNA.
DR EMBL; BC029755; AAH29755.1; -; mRNA.
DR EMBL; AL833010; CAH56291.1; -; mRNA.
DR RefSeq; NP_004701.1; NM_004710.3.
DR UniGene; Hs.464210; -.
DR UniGene; Hs.708616; -.
DR ProteinModelPortal; O43760; -.
DR IntAct; O43760; 1.
DR STRING; 9606.ENSP00000225777; -.
DR PhosphoSite; O43760; -.
DR PaxDb; O43760; -.
DR PRIDE; O43760; -.
DR DNASU; 9144; -.
DR Ensembl; ENST00000225777; ENSP00000225777; ENSG00000108639.
DR Ensembl; ENST00000585591; ENSP00000465678; ENSG00000108639.
DR GeneID; 9144; -.
DR KEGG; hsa:9144; -.
DR UCSC; uc002juu.1; human.
DR CTD; 9144; -.
DR GeneCards; GC17P076164; -.
DR HGNC; HGNC:11499; SYNGR2.
DR HPA; HPA014742; -.
DR MIM; 603926; gene.
DR neXtProt; NX_O43760; -.
DR PharmGKB; PA36281; -.
DR eggNOG; NOG306774; -.
DR HOGENOM; HOG000220816; -.
DR HOVERGEN; HBG000896; -.
DR InParanoid; O43760; -.
DR PhylomeDB; O43760; -.
DR GenomeRNAi; 9144; -.
DR NextBio; 34295; -.
DR PRO; PR:O43760; -.
DR ArrayExpress; O43760; -.
DR Bgee; O43760; -.
DR CleanEx; HS_SYNGR2; -.
DR Genevestigator; O43760; -.
DR GO; GO:0016021; C:integral to membrane; NAS:UniProtKB.
DR GO; GO:0008021; C:synaptic vesicle; IEA:Ensembl.
DR GO; GO:0006605; P:protein targeting; IEA:Ensembl.
DR InterPro; IPR008253; Marvel.
DR InterPro; IPR016579; Synaptogyrin.
DR Pfam; PF01284; MARVEL; 1.
DR PIRSF; PIRSF011282; Synaptogyrin; 1.
DR PROSITE; PS51225; MARVEL; 1.
PE 1: Evidence at protein level;
KW Acetylation; Complete proteome; Cytoplasmic vesicle;
KW Direct protein sequencing; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1 224 Synaptogyrin-2.
FT /FTId=PRO_0000183993.
FT TRANSMEM 26 46 Helical; (Potential).
FT TRANSMEM 73 93 Helical; (Potential).
FT TRANSMEM 105 125 Helical; (Potential).
FT TRANSMEM 147 167 Helical; (Potential).
FT DOMAIN 20 171 MARVEL.
FT MOD_RES 1 1 N-acetylmethionine.
FT CONFLICT 160 162 GVL -> VGW (in Ref. 1; CAA05327).
SQ SEQUENCE 224 AA; 24810 MW; EC92C95CE9E5BD41 CRC64;
MESGAYGAAK AGGSFDLRRF LTQPQVVARA VCLVFALIVF SCIYGEGYSN AHESKQMYCV
FNRNEDACRY GSAIGVLAFL ASAFFLVVDA YFPQISNATD RKYLVIGDLL FSALWTFLWF
VGFCFLTNQW AVTNPKDVLV GADSVRAAIT FSFFSIFSWG VLASLAYQRY KAGVDDFIQN
YVDPTPDPNT AYASYPGASV DNYQQPPFTQ NAETTEGYQP PPVY
//
MIM
603926
*RECORD*
*FIELD* NO
603926
*FIELD* TI
*603926 SYNAPTOGYRIN 2; SYNGR2
;;CELLUGYRIN
*FIELD* TX
DESCRIPTION
Synaptic vesicles represent specialized secretory organelles that store
read moreneurotransmitters in nerve terminals, and release them by fusing with
the presynaptic plasma membrane during exocytosis. Synaptogyrin (SYNGR1;
603925) and synaptophysins I (see 313475) and II (synaptoporin) contain
4 transmembrane regions with cytoplasmic C and N termini, and are among
the most abundant synaptic vesicle proteins.
CLONING
Janz and Sudhof (1998) identified a rat gene encoding cellugyrin, a
protein related to synaptogyrin. Western blot analysis revealed that
cellugyrin and synaptogyrin are expressed in mirror-image patterns in
rat tissues: cellugyrin is ubiquitous with the lowest levels in brain,
while synaptogyrin is detectable only in the brain. Subcellular
fractionation of rat brain cells indicated that both synaptogyrin and
cellugyrin are associated with microsomes; however, only synaptogyrin is
found in synaptic vesicles. The authors suggested that synaptogyrin is a
specialized version of the ubiquitous cellugyrin, supporting the concept
that synaptic vesicles are a simplified and specialized form of a
generic trafficking organelle. Independently, Kedra et al. (1998)
identified cDNAs encoding the mouse and human homologs of cellugyrin,
which they called synaptogyrin- 2 (SYNGR2). Like SYNGR1, the predicted
224-amino acid human SYNGR2 protein contains 4 transmembrane regions.
Northern blot analysis revealed that the 1.6-kb SYNGR2 mRNA was
expressed at high levels in all tissues tested except brain.
MAPPING
By fluorescence in situ hybridization and by analysis of mapped clones,
Kedra et al. (1998) mapped the SYNGR2 gene to 17qtel and an SYNGR2
pseudogene to 15q11.
*FIELD* RF
1. Janz, R.; Sudhof, T. C.: Cellugyrin, a novel ubiquitous form of
synaptogyrin that is phosphorylated by pp60(c-src). J. Biol. Chem. 273:
2851-2857, 1998.
2. Kedra, D.; Pan, H.-Q.; Seroussi, E.; Fransson, I.; Guilbaud, C.;
Collins, J. E.; Dunham, I.; Blennow, E.; Roe, B. A.; Piehl, F.; Dumanski,
J. P.: Characterization of the human synaptogyrin gene family. Hum.
Genet. 103: 131-141, 1998.
*FIELD* CD
Rebekah S. Rasooly: 6/18/1999
*FIELD* ED
carol: 11/28/2001
jlewis: 6/22/1999
*RECORD*
*FIELD* NO
603926
*FIELD* TI
*603926 SYNAPTOGYRIN 2; SYNGR2
;;CELLUGYRIN
*FIELD* TX
DESCRIPTION
Synaptic vesicles represent specialized secretory organelles that store
read moreneurotransmitters in nerve terminals, and release them by fusing with
the presynaptic plasma membrane during exocytosis. Synaptogyrin (SYNGR1;
603925) and synaptophysins I (see 313475) and II (synaptoporin) contain
4 transmembrane regions with cytoplasmic C and N termini, and are among
the most abundant synaptic vesicle proteins.
CLONING
Janz and Sudhof (1998) identified a rat gene encoding cellugyrin, a
protein related to synaptogyrin. Western blot analysis revealed that
cellugyrin and synaptogyrin are expressed in mirror-image patterns in
rat tissues: cellugyrin is ubiquitous with the lowest levels in brain,
while synaptogyrin is detectable only in the brain. Subcellular
fractionation of rat brain cells indicated that both synaptogyrin and
cellugyrin are associated with microsomes; however, only synaptogyrin is
found in synaptic vesicles. The authors suggested that synaptogyrin is a
specialized version of the ubiquitous cellugyrin, supporting the concept
that synaptic vesicles are a simplified and specialized form of a
generic trafficking organelle. Independently, Kedra et al. (1998)
identified cDNAs encoding the mouse and human homologs of cellugyrin,
which they called synaptogyrin- 2 (SYNGR2). Like SYNGR1, the predicted
224-amino acid human SYNGR2 protein contains 4 transmembrane regions.
Northern blot analysis revealed that the 1.6-kb SYNGR2 mRNA was
expressed at high levels in all tissues tested except brain.
MAPPING
By fluorescence in situ hybridization and by analysis of mapped clones,
Kedra et al. (1998) mapped the SYNGR2 gene to 17qtel and an SYNGR2
pseudogene to 15q11.
*FIELD* RF
1. Janz, R.; Sudhof, T. C.: Cellugyrin, a novel ubiquitous form of
synaptogyrin that is phosphorylated by pp60(c-src). J. Biol. Chem. 273:
2851-2857, 1998.
2. Kedra, D.; Pan, H.-Q.; Seroussi, E.; Fransson, I.; Guilbaud, C.;
Collins, J. E.; Dunham, I.; Blennow, E.; Roe, B. A.; Piehl, F.; Dumanski,
J. P.: Characterization of the human synaptogyrin gene family. Hum.
Genet. 103: 131-141, 1998.
*FIELD* CD
Rebekah S. Rasooly: 6/18/1999
*FIELD* ED
carol: 11/28/2001
jlewis: 6/22/1999