Full text data of SSSCA1
SSSCA1
[Confidence: low (only semi-automatic identification from reviews)]
Sjoegren syndrome/scleroderma autoantigen 1 (Autoantigen p27)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Sjoegren syndrome/scleroderma autoantigen 1 (Autoantigen p27)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
O60232
ID SSA27_HUMAN Reviewed; 199 AA.
AC O60232;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-AUG-1998, sequence version 1.
DT 22-JAN-2014, entry version 110.
DE RecName: Full=Sjoegren syndrome/scleroderma autoantigen 1;
DE AltName: Full=Autoantigen p27;
GN Name=SSSCA1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9486406; DOI=10.1046/j.1365-2249.1998.00517.x;
RA Muro Y., Yamada T., Himeno M., Sugimoto K.;
RT "cDNA cloning of a novel autoantigen targeted by a minor subset of
RT anti-centromere antibodies.";
RL Clin. Exp. Immunol. 111:372-376(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, AND MASS
RP SPECTROMETRY.
RC TISSUE=Embryonic kidney;
RX PubMed=17525332; DOI=10.1126/science.1140321;
RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,
RA Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,
RA Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
RT "ATM and ATR substrate analysis reveals extensive protein networks
RT responsive to DNA damage.";
RL Science 316:1160-1166(2007).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Might play a role in mitosis. Antigenic molecule. Could
CC be a centromere-associated protein. May induce anti-centromere
CC antibodies.
CC -!- INTERACTION:
CC Q9Q2G4:ORF (xeno); NbExp=4; IntAct=EBI-741415, EBI-6248094;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB001740; BAA25263.1; -; mRNA.
DR EMBL; BC014791; AAH14791.1; -; mRNA.
DR RefSeq; NP_006387.1; NM_006396.1.
DR UniGene; Hs.25723; -.
DR ProteinModelPortal; O60232; -.
DR IntAct; O60232; 15.
DR MINT; MINT-1434192; -.
DR STRING; 9606.ENSP00000312318; -.
DR PhosphoSite; O60232; -.
DR PaxDb; O60232; -.
DR PeptideAtlas; O60232; -.
DR PRIDE; O60232; -.
DR DNASU; 10534; -.
DR Ensembl; ENST00000309328; ENSP00000312318; ENSG00000173465.
DR GeneID; 10534; -.
DR KEGG; hsa:10534; -.
DR UCSC; uc001oek.3; human.
DR CTD; 10534; -.
DR GeneCards; GC11P065337; -.
DR HGNC; HGNC:11328; SSSCA1.
DR HPA; HPA039789; -.
DR MIM; 606044; gene.
DR neXtProt; NX_O60232; -.
DR PharmGKB; PA36152; -.
DR eggNOG; NOG303301; -.
DR HOGENOM; HOG000007383; -.
DR HOVERGEN; HBG054236; -.
DR InParanoid; O60232; -.
DR OMA; MKVIQAR; -.
DR PhylomeDB; O60232; -.
DR ChiTaRS; SSSCA1; human.
DR GenomeRNAi; 10534; -.
DR NextBio; 39965; -.
DR PMAP-CutDB; O60232; -.
DR PRO; PR:O60232; -.
DR ArrayExpress; O60232; -.
DR Bgee; O60232; -.
DR CleanEx; HS_SSSCA1; -.
DR Genevestigator; O60232; -.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007067; P:mitosis; TAS:ProtInc.
DR InterPro; IPR009563; SSSCA1.
DR Pfam; PF06677; Auto_anti-p27; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Complete proteome; Mitosis;
KW Phosphoprotein; Polymorphism; Reference proteome.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 199 Sjoegren syndrome/scleroderma autoantigen
FT 1.
FT /FTId=PRO_0000072201.
FT COMPBIAS 75 83 Asp/Glu-rich (acidic).
FT COMPBIAS 113 122 Pro-rich.
FT COMPBIAS 138 145 Pro-rich.
FT COMPBIAS 170 177 Ser/Thr-rich.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 94 94 Phosphoserine.
FT VARIANT 21 21 T -> M (in dbSNP:rs35971725).
FT /FTId=VAR_051383.
SQ SEQUENCE 199 AA; 21474 MW; EF08439FDFFA1DAB CRC64;
MALNGAEVDD FSWEPPTEAE TKVLQARRER QDRISRLMGD YLLRGYRMLG ETCADCGTIL
LQDKQRKIYC VACQELDSDV DKDNPALNAQ AALSQAREHQ LASASELPLG SRPAPQPPVP
RPEHCEGAAA GLKAAQGPPA PAVPPNTDVM ACTQTALLQK LTWASAELGS STSLETSIQL
CGLIRACAEA LRSLQQLQH
//
ID SSA27_HUMAN Reviewed; 199 AA.
AC O60232;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-AUG-1998, sequence version 1.
DT 22-JAN-2014, entry version 110.
DE RecName: Full=Sjoegren syndrome/scleroderma autoantigen 1;
DE AltName: Full=Autoantigen p27;
GN Name=SSSCA1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9486406; DOI=10.1046/j.1365-2249.1998.00517.x;
RA Muro Y., Yamada T., Himeno M., Sugimoto K.;
RT "cDNA cloning of a novel autoantigen targeted by a minor subset of
RT anti-centromere antibodies.";
RL Clin. Exp. Immunol. 111:372-376(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, AND MASS
RP SPECTROMETRY.
RC TISSUE=Embryonic kidney;
RX PubMed=17525332; DOI=10.1126/science.1140321;
RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,
RA Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,
RA Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
RT "ATM and ATR substrate analysis reveals extensive protein networks
RT responsive to DNA damage.";
RL Science 316:1160-1166(2007).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Might play a role in mitosis. Antigenic molecule. Could
CC be a centromere-associated protein. May induce anti-centromere
CC antibodies.
CC -!- INTERACTION:
CC Q9Q2G4:ORF (xeno); NbExp=4; IntAct=EBI-741415, EBI-6248094;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB001740; BAA25263.1; -; mRNA.
DR EMBL; BC014791; AAH14791.1; -; mRNA.
DR RefSeq; NP_006387.1; NM_006396.1.
DR UniGene; Hs.25723; -.
DR ProteinModelPortal; O60232; -.
DR IntAct; O60232; 15.
DR MINT; MINT-1434192; -.
DR STRING; 9606.ENSP00000312318; -.
DR PhosphoSite; O60232; -.
DR PaxDb; O60232; -.
DR PeptideAtlas; O60232; -.
DR PRIDE; O60232; -.
DR DNASU; 10534; -.
DR Ensembl; ENST00000309328; ENSP00000312318; ENSG00000173465.
DR GeneID; 10534; -.
DR KEGG; hsa:10534; -.
DR UCSC; uc001oek.3; human.
DR CTD; 10534; -.
DR GeneCards; GC11P065337; -.
DR HGNC; HGNC:11328; SSSCA1.
DR HPA; HPA039789; -.
DR MIM; 606044; gene.
DR neXtProt; NX_O60232; -.
DR PharmGKB; PA36152; -.
DR eggNOG; NOG303301; -.
DR HOGENOM; HOG000007383; -.
DR HOVERGEN; HBG054236; -.
DR InParanoid; O60232; -.
DR OMA; MKVIQAR; -.
DR PhylomeDB; O60232; -.
DR ChiTaRS; SSSCA1; human.
DR GenomeRNAi; 10534; -.
DR NextBio; 39965; -.
DR PMAP-CutDB; O60232; -.
DR PRO; PR:O60232; -.
DR ArrayExpress; O60232; -.
DR Bgee; O60232; -.
DR CleanEx; HS_SSSCA1; -.
DR Genevestigator; O60232; -.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007067; P:mitosis; TAS:ProtInc.
DR InterPro; IPR009563; SSSCA1.
DR Pfam; PF06677; Auto_anti-p27; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Complete proteome; Mitosis;
KW Phosphoprotein; Polymorphism; Reference proteome.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 199 Sjoegren syndrome/scleroderma autoantigen
FT 1.
FT /FTId=PRO_0000072201.
FT COMPBIAS 75 83 Asp/Glu-rich (acidic).
FT COMPBIAS 113 122 Pro-rich.
FT COMPBIAS 138 145 Pro-rich.
FT COMPBIAS 170 177 Ser/Thr-rich.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 94 94 Phosphoserine.
FT VARIANT 21 21 T -> M (in dbSNP:rs35971725).
FT /FTId=VAR_051383.
SQ SEQUENCE 199 AA; 21474 MW; EF08439FDFFA1DAB CRC64;
MALNGAEVDD FSWEPPTEAE TKVLQARRER QDRISRLMGD YLLRGYRMLG ETCADCGTIL
LQDKQRKIYC VACQELDSDV DKDNPALNAQ AALSQAREHQ LASASELPLG SRPAPQPPVP
RPEHCEGAAA GLKAAQGPPA PAVPPNTDVM ACTQTALLQK LTWASAELGS STSLETSIQL
CGLIRACAEA LRSLQQLQH
//
MIM
606044
*RECORD*
*FIELD* NO
606044
*FIELD* TI
*606044 SJOGREN SYNDROME/SCLERODERMA AUTOANTIGEN 1; SSSCA1
;;CENTROMERIC AUTOANTIGEN, 27-KD
read more*FIELD* TX
CLONING
The centromere plays an essential role in the pairing and partitioning
of replicated chromosomes in mitosis and meiosis and is recognized by
anticentromere antibodies in patients with autoimmune disorders. By
immunoscreening a cDNA library with serum from a Sjogren syndrome
(270150) patient with anticentromere antibodies, Muro et al. (1998)
isolated a cDNA encoding SSSCA1, which they referred to as p27 due to
its molecular mass in Western blot analysis. The deduced 199-amino acid,
leucine-rich protein has an N terminus with many charged residues and a
potential phosphorylation site; 2 short proline-rich stretches; and a
potential C-terminal phosphorylation site. Immunofluorescence analysis
failed to demonstrate centromeric or other staining. Immunoblot analysis
with recombinant SSSCA1 determined that approximately 2% of patients
with anticentromere antibodies had anti-SSSCA1 antibodies, while less
than 1% of autoimmune patients without anticentromere antibodies had
anti-SSSCA1 antibodies. All 5 seropositive patients had a diagnosis of
scleroderma (see 181750) or Sjogren syndrome with internal organ
involvement.
*FIELD* RF
1. Muro, Y.; Yamada, T.; Himeno, M.; Sugimoto, K.: cDNA cloning of
a novel autoantigen targeted by a minor subset of anti-centromere
antibodies. Clin. Exp. Immun. 111: 372-376, 1998.
*FIELD* CD
Paul J. Converse: 6/20/2001
*FIELD* ED
alopez: 04/04/2012
mgross: 6/20/2001
*RECORD*
*FIELD* NO
606044
*FIELD* TI
*606044 SJOGREN SYNDROME/SCLERODERMA AUTOANTIGEN 1; SSSCA1
;;CENTROMERIC AUTOANTIGEN, 27-KD
read more*FIELD* TX
CLONING
The centromere plays an essential role in the pairing and partitioning
of replicated chromosomes in mitosis and meiosis and is recognized by
anticentromere antibodies in patients with autoimmune disorders. By
immunoscreening a cDNA library with serum from a Sjogren syndrome
(270150) patient with anticentromere antibodies, Muro et al. (1998)
isolated a cDNA encoding SSSCA1, which they referred to as p27 due to
its molecular mass in Western blot analysis. The deduced 199-amino acid,
leucine-rich protein has an N terminus with many charged residues and a
potential phosphorylation site; 2 short proline-rich stretches; and a
potential C-terminal phosphorylation site. Immunofluorescence analysis
failed to demonstrate centromeric or other staining. Immunoblot analysis
with recombinant SSSCA1 determined that approximately 2% of patients
with anticentromere antibodies had anti-SSSCA1 antibodies, while less
than 1% of autoimmune patients without anticentromere antibodies had
anti-SSSCA1 antibodies. All 5 seropositive patients had a diagnosis of
scleroderma (see 181750) or Sjogren syndrome with internal organ
involvement.
*FIELD* RF
1. Muro, Y.; Yamada, T.; Himeno, M.; Sugimoto, K.: cDNA cloning of
a novel autoantigen targeted by a minor subset of anti-centromere
antibodies. Clin. Exp. Immun. 111: 372-376, 1998.
*FIELD* CD
Paul J. Converse: 6/20/2001
*FIELD* ED
alopez: 04/04/2012
mgross: 6/20/2001