Full text data of STX18
STX18
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Syntaxin-18 (Cell growth-inhibiting gene 9 protein)
Syntaxin-18 (Cell growth-inhibiting gene 9 protein)
UniProt
Q9P2W9
ID STX18_HUMAN Reviewed; 335 AA.
AC Q9P2W9; Q596L3; Q5TZP5;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 107.
DE RecName: Full=Syntaxin-18;
DE AltName: Full=Cell growth-inhibiting gene 9 protein;
GN Name=STX18; ORFNames=GIG9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=10788491; DOI=10.1074/jbc.275.18.13713;
RA Hatsuzawa K., Hirose H., Tani K., Yamamoto A., Scheller R.H.,
RA Tagaya M.;
RT "Syntaxin 18, a SNAP receptor that functions in the endoplasmic
RT reticulum, intermediate compartment, and cis-Golgi vesicle
RT trafficking.";
RL J. Biol. Chem. 275:13713-13720(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kim J.W.;
RT "Identification of a human growth inhibiting gene.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION,
RP AND IDENTIFICATION IN A COMPLEX WITH USE1L; SEC22B; RINT1 AND ZW10.
RX PubMed=15029241; DOI=10.1038/sj.emboj.7600135;
RA Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M.,
RA Tani K., Yamamoto A., Tohyama M., Tagaya M.;
RT "Implication of ZW10 in membrane trafficking between the endoplasmic
RT reticulum and Golgi.";
RL EMBO J. 23:1267-1278(2004).
RN [6]
RP INTERACTION WITH BNIP1.
RX PubMed=15272311; DOI=10.1038/sj.emboj.7600333;
RA Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K.,
RA Nagahama M., Tani K., Yamamoto A., Tagaya M.;
RT "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane
RT fusion.";
RL EMBO J. 23:3216-3226(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Syntaxin that may be involved in targeting and fusion of
CC Golgi-derived retrograde transport vesicles with the ER.
CC -!- SUBUNIT: Component of a SNARE complex consisting of STX18, USE1L,
CC BNIP1/SEC20L, and SEC22B. RINT1/TIP20L and ZW10 are associated
CC with the complex through interaction with BNIP1/SEC20L. Interacts
CC directly with USE1L and BNIP1/SEC20L.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC type IV membrane protein (Probable). Golgi apparatus membrane;
CC Single-pass type IV membrane protein (Potential).
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the syntaxin family.
CC -!- SIMILARITY: Contains 1 t-SNARE coiled-coil homology domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB028741; BAA95213.1; -; mRNA.
DR EMBL; AY453396; AAS47513.1; -; mRNA.
DR EMBL; BT007150; AAP35814.1; -; mRNA.
DR EMBL; BT020135; AAV38937.1; -; mRNA.
DR EMBL; BC014613; AAH14613.1; -; mRNA.
DR RefSeq; NP_058626.1; NM_016930.2.
DR UniGene; Hs.584913; -.
DR ProteinModelPortal; Q9P2W9; -.
DR SMR; Q9P2W9; 245-307.
DR IntAct; Q9P2W9; 3.
DR MINT; MINT-1378736; -.
DR STRING; 9606.ENSP00000305810; -.
DR PhosphoSite; Q9P2W9; -.
DR DMDM; 17369347; -.
DR PaxDb; Q9P2W9; -.
DR PRIDE; Q9P2W9; -.
DR DNASU; 53407; -.
DR Ensembl; ENST00000306200; ENSP00000305810; ENSG00000168818.
DR GeneID; 53407; -.
DR KEGG; hsa:53407; -.
DR UCSC; uc003gic.3; human.
DR CTD; 53407; -.
DR GeneCards; GC04M004417; -.
DR H-InvDB; HIX0120162; -.
DR HGNC; HGNC:15942; STX18.
DR HPA; HPA003019; -.
DR MIM; 606046; gene.
DR neXtProt; NX_Q9P2W9; -.
DR PharmGKB; PA38061; -.
DR eggNOG; NOG271515; -.
DR HOGENOM; HOG000007595; -.
DR HOVERGEN; HBG054890; -.
DR InParanoid; Q9P2W9; -.
DR KO; K08492; -.
DR OMA; IFMRTCA; -.
DR PhylomeDB; Q9P2W9; -.
DR GenomeRNAi; 53407; -.
DR NextBio; 56080; -.
DR PRO; PR:Q9P2W9; -.
DR ArrayExpress; Q9P2W9; -.
DR Bgee; Q9P2W9; -.
DR CleanEx; HS_STX18; -.
DR Genevestigator; Q9P2W9; -.
DR GO; GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; TAS:ProtInc.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR InterPro; IPR019529; Syntaxin-18_N.
DR InterPro; IPR006012; Syntaxin/epimorphin_CS.
DR Pfam; PF10496; Syntaxin-18_N; 1.
DR PROSITE; PS00914; SYNTAXIN; 1.
DR PROSITE; PS50192; T_SNARE; FALSE_NEG.
PE 1: Evidence at protein level;
KW Coiled coil; Complete proteome; Endoplasmic reticulum;
KW ER-Golgi transport; Golgi apparatus; Membrane; Polymorphism;
KW Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1 335 Syntaxin-18.
FT /FTId=PRO_0000210231.
FT TOPO_DOM 1 309 Cytoplasmic (Potential).
FT TRANSMEM 310 330 Helical; Anchor for type IV membrane
FT protein; (Potential).
FT TOPO_DOM 331 335 Vesicular (Potential).
FT DOMAIN 243 305 t-SNARE coiled-coil homology.
FT VARIANT 32 32 D -> Y (in dbSNP:rs13134070).
FT /FTId=VAR_052250.
FT VARIANT 51 51 S -> G (in dbSNP:rs36109375).
FT /FTId=VAR_052251.
FT VARIANT 228 228 S -> T (in dbSNP:rs33952588).
FT /FTId=VAR_052252.
FT CONFLICT 11 11 A -> V (in Ref. 3; AAV38937).
FT CONFLICT 308 308 N -> D (in Ref. 2; AAS47513).
SQ SEQUENCE 335 AA; 38674 MW; D89B3B52407D77FF CRC64;
MAVDITLLFR ASVKTVKTRN KALGVAVGGG VDGSRDELFR RSPRPKGDFS SRAREVISHI
GKLRDFLLEH RKDYINAYSH TMSEYGRMTD TERDQIDQDA QIFMRTCSEA IQQLRTEAHK
EIHSQQVKEH RTAVLDFIED YLKRVCKLYS EQRAIRVKRV VDKKRLSKLE PEPNTKTRES
TSSEKVSQSP SKDSEENPAT EERPEKILAE TQPELGTWGD GKGEDELSPE EIQMFEQENQ
RLIGEMNSLF DEVRQIEGRV VEISRLQEIF TEKVLQQEAE IDSIHQLVVG ATENIKEGNE
DIREAIKNNA GFRVWILFFL VMCSFSLLFL DWYDS
//
ID STX18_HUMAN Reviewed; 335 AA.
AC Q9P2W9; Q596L3; Q5TZP5;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 107.
DE RecName: Full=Syntaxin-18;
DE AltName: Full=Cell growth-inhibiting gene 9 protein;
GN Name=STX18; ORFNames=GIG9;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=10788491; DOI=10.1074/jbc.275.18.13713;
RA Hatsuzawa K., Hirose H., Tani K., Yamamoto A., Scheller R.H.,
RA Tagaya M.;
RT "Syntaxin 18, a SNAP receptor that functions in the endoplasmic
RT reticulum, intermediate compartment, and cis-Golgi vesicle
RT trafficking.";
RL J. Biol. Chem. 275:13713-13720(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kim J.W.;
RT "Identification of a human growth inhibiting gene.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION,
RP AND IDENTIFICATION IN A COMPLEX WITH USE1L; SEC22B; RINT1 AND ZW10.
RX PubMed=15029241; DOI=10.1038/sj.emboj.7600135;
RA Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M.,
RA Tani K., Yamamoto A., Tohyama M., Tagaya M.;
RT "Implication of ZW10 in membrane trafficking between the endoplasmic
RT reticulum and Golgi.";
RL EMBO J. 23:1267-1278(2004).
RN [6]
RP INTERACTION WITH BNIP1.
RX PubMed=15272311; DOI=10.1038/sj.emboj.7600333;
RA Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K.,
RA Nagahama M., Tani K., Yamamoto A., Tagaya M.;
RT "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane
RT fusion.";
RL EMBO J. 23:3216-3226(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Syntaxin that may be involved in targeting and fusion of
CC Golgi-derived retrograde transport vesicles with the ER.
CC -!- SUBUNIT: Component of a SNARE complex consisting of STX18, USE1L,
CC BNIP1/SEC20L, and SEC22B. RINT1/TIP20L and ZW10 are associated
CC with the complex through interaction with BNIP1/SEC20L. Interacts
CC directly with USE1L and BNIP1/SEC20L.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC type IV membrane protein (Probable). Golgi apparatus membrane;
CC Single-pass type IV membrane protein (Potential).
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- SIMILARITY: Belongs to the syntaxin family.
CC -!- SIMILARITY: Contains 1 t-SNARE coiled-coil homology domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AB028741; BAA95213.1; -; mRNA.
DR EMBL; AY453396; AAS47513.1; -; mRNA.
DR EMBL; BT007150; AAP35814.1; -; mRNA.
DR EMBL; BT020135; AAV38937.1; -; mRNA.
DR EMBL; BC014613; AAH14613.1; -; mRNA.
DR RefSeq; NP_058626.1; NM_016930.2.
DR UniGene; Hs.584913; -.
DR ProteinModelPortal; Q9P2W9; -.
DR SMR; Q9P2W9; 245-307.
DR IntAct; Q9P2W9; 3.
DR MINT; MINT-1378736; -.
DR STRING; 9606.ENSP00000305810; -.
DR PhosphoSite; Q9P2W9; -.
DR DMDM; 17369347; -.
DR PaxDb; Q9P2W9; -.
DR PRIDE; Q9P2W9; -.
DR DNASU; 53407; -.
DR Ensembl; ENST00000306200; ENSP00000305810; ENSG00000168818.
DR GeneID; 53407; -.
DR KEGG; hsa:53407; -.
DR UCSC; uc003gic.3; human.
DR CTD; 53407; -.
DR GeneCards; GC04M004417; -.
DR H-InvDB; HIX0120162; -.
DR HGNC; HGNC:15942; STX18.
DR HPA; HPA003019; -.
DR MIM; 606046; gene.
DR neXtProt; NX_Q9P2W9; -.
DR PharmGKB; PA38061; -.
DR eggNOG; NOG271515; -.
DR HOGENOM; HOG000007595; -.
DR HOVERGEN; HBG054890; -.
DR InParanoid; Q9P2W9; -.
DR KO; K08492; -.
DR OMA; IFMRTCA; -.
DR PhylomeDB; Q9P2W9; -.
DR GenomeRNAi; 53407; -.
DR NextBio; 56080; -.
DR PRO; PR:Q9P2W9; -.
DR ArrayExpress; Q9P2W9; -.
DR Bgee; Q9P2W9; -.
DR CleanEx; HS_STX18; -.
DR Genevestigator; Q9P2W9; -.
DR GO; GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; TAS:ProtInc.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR InterPro; IPR019529; Syntaxin-18_N.
DR InterPro; IPR006012; Syntaxin/epimorphin_CS.
DR Pfam; PF10496; Syntaxin-18_N; 1.
DR PROSITE; PS00914; SYNTAXIN; 1.
DR PROSITE; PS50192; T_SNARE; FALSE_NEG.
PE 1: Evidence at protein level;
KW Coiled coil; Complete proteome; Endoplasmic reticulum;
KW ER-Golgi transport; Golgi apparatus; Membrane; Polymorphism;
KW Protein transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1 335 Syntaxin-18.
FT /FTId=PRO_0000210231.
FT TOPO_DOM 1 309 Cytoplasmic (Potential).
FT TRANSMEM 310 330 Helical; Anchor for type IV membrane
FT protein; (Potential).
FT TOPO_DOM 331 335 Vesicular (Potential).
FT DOMAIN 243 305 t-SNARE coiled-coil homology.
FT VARIANT 32 32 D -> Y (in dbSNP:rs13134070).
FT /FTId=VAR_052250.
FT VARIANT 51 51 S -> G (in dbSNP:rs36109375).
FT /FTId=VAR_052251.
FT VARIANT 228 228 S -> T (in dbSNP:rs33952588).
FT /FTId=VAR_052252.
FT CONFLICT 11 11 A -> V (in Ref. 3; AAV38937).
FT CONFLICT 308 308 N -> D (in Ref. 2; AAS47513).
SQ SEQUENCE 335 AA; 38674 MW; D89B3B52407D77FF CRC64;
MAVDITLLFR ASVKTVKTRN KALGVAVGGG VDGSRDELFR RSPRPKGDFS SRAREVISHI
GKLRDFLLEH RKDYINAYSH TMSEYGRMTD TERDQIDQDA QIFMRTCSEA IQQLRTEAHK
EIHSQQVKEH RTAVLDFIED YLKRVCKLYS EQRAIRVKRV VDKKRLSKLE PEPNTKTRES
TSSEKVSQSP SKDSEENPAT EERPEKILAE TQPELGTWGD GKGEDELSPE EIQMFEQENQ
RLIGEMNSLF DEVRQIEGRV VEISRLQEIF TEKVLQQEAE IDSIHQLVVG ATENIKEGNE
DIREAIKNNA GFRVWILFFL VMCSFSLLFL DWYDS
//
MIM
606046
*RECORD*
*FIELD* NO
606046
*FIELD* TI
*606046 SYNTAXIN 18; STX18
*FIELD* TX
In eukaryotic cells, SNARE proteins (SNAP receptors) mediate the docking
read moreand/or fusion of transport vesicles with target membranes. See
alpha-SNAP (NAPA; 603215). V-SNARES, found on vesicles, interact with
t-SNARES (syntaxins), found on target membranes, in a specific manner.
CLONING
Using a yeast 2-hybrid screen with NAPA cDNA as bait, Hatsuzawa et al.
(2000) identified a partial human cDNA encoding a novel syntaxin, which
they called syntaxin-18. They isolated a complete cDNA by screening a
human brain cDNA library. The deduced 335-amino acid protein contains
elements similar to other syntaxins, including predicted coiled-coil
regions and a putative transmembrane domain near the C terminus.
Syntaxin-18 shares 28% sequence identity with the C. elegans T10H9
predicted gene product, but low sequence similarity to other known
syntaxins. Northern blot analysis detected ubiquitous expression of a
1.8-kb STX18 transcript. Using subcellular fractionation and
immunofluorescence, Hatsuzawa et al. (2000) showed that syntaxin-18 is
principally located in the endoplasmic reticulum.
GENE FUNCTION
Based on in vivo overexpression and protein transport studies using
epitope-tagged proteins, Hatsuzawa et al. (2000) suggested that
syntaxin-18 functions in transport between the endoplasmic reticulum and
Golgi.
*FIELD* RF
1. Hatsuzawa, K.; Hirose, H.; Tani, K.; Yamamoto, A.; Scheller, R.
H.; Tagaya, M.: Syntaxin 18, a SNAP receptor that functions in the
endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle
trafficking. J. Biol. Chem. 275: 13713-13720, 2000.
*FIELD* CD
Tara S. Marathe: 6/20/2001
*FIELD* ED
alopez: 01/02/2007
carol: 3/8/2002
carol: 6/20/2001
*RECORD*
*FIELD* NO
606046
*FIELD* TI
*606046 SYNTAXIN 18; STX18
*FIELD* TX
In eukaryotic cells, SNARE proteins (SNAP receptors) mediate the docking
read moreand/or fusion of transport vesicles with target membranes. See
alpha-SNAP (NAPA; 603215). V-SNARES, found on vesicles, interact with
t-SNARES (syntaxins), found on target membranes, in a specific manner.
CLONING
Using a yeast 2-hybrid screen with NAPA cDNA as bait, Hatsuzawa et al.
(2000) identified a partial human cDNA encoding a novel syntaxin, which
they called syntaxin-18. They isolated a complete cDNA by screening a
human brain cDNA library. The deduced 335-amino acid protein contains
elements similar to other syntaxins, including predicted coiled-coil
regions and a putative transmembrane domain near the C terminus.
Syntaxin-18 shares 28% sequence identity with the C. elegans T10H9
predicted gene product, but low sequence similarity to other known
syntaxins. Northern blot analysis detected ubiquitous expression of a
1.8-kb STX18 transcript. Using subcellular fractionation and
immunofluorescence, Hatsuzawa et al. (2000) showed that syntaxin-18 is
principally located in the endoplasmic reticulum.
GENE FUNCTION
Based on in vivo overexpression and protein transport studies using
epitope-tagged proteins, Hatsuzawa et al. (2000) suggested that
syntaxin-18 functions in transport between the endoplasmic reticulum and
Golgi.
*FIELD* RF
1. Hatsuzawa, K.; Hirose, H.; Tani, K.; Yamamoto, A.; Scheller, R.
H.; Tagaya, M.: Syntaxin 18, a SNAP receptor that functions in the
endoplasmic reticulum, intermediate compartment, and cis-Golgi vesicle
trafficking. J. Biol. Chem. 275: 13713-13720, 2000.
*FIELD* CD
Tara S. Marathe: 6/20/2001
*FIELD* ED
alopez: 01/02/2007
carol: 3/8/2002
carol: 6/20/2001