Full text data of NARS
NARS
[Confidence: low (only semi-automatic identification from reviews)]
Asparagine--tRNA ligase, cytoplasmic; 6.1.1.22 (Asparaginyl-tRNA synthetase; AsnRS)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Asparagine--tRNA ligase, cytoplasmic; 6.1.1.22 (Asparaginyl-tRNA synthetase; AsnRS)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
O43776
ID SYNC_HUMAN Reviewed; 548 AA.
AC O43776; Q53GU6;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-1998, sequence version 1.
DT 22-JAN-2014, entry version 123.
DE RecName: Full=Asparagine--tRNA ligase, cytoplasmic;
DE EC=6.1.1.22;
DE AltName: Full=Asparaginyl-tRNA synthetase;
DE Short=AsnRS;
GN Name=NARS;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=9421509; DOI=10.1093/nar/26.2.521;
RA Beaulande M., Tarbouriech N., Haertlein M.;
RT "Human cytosolic asparaginyl-tRNA synthetase: cDNA sequence,
RT functional expression in Escherichia coli and characterization as
RT human autoantigen.";
RL Nucleic Acids Res. 26:521-524(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9801298; DOI=10.1093/nar/26.22.5045;
RA Shiba K., Motegi H., Yoshida M., Noda T.;
RT "Human asparaginyl-tRNA synthetase: molecular cloning and the
RT inference of the evolutionary history of Asx-tRNA synthetase family.";
RL Nucleic Acids Res. 26:5045-5051(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 2-12.
RC TISSUE=Leukemic T-cell;
RX PubMed=19892738; DOI=10.1073/pnas.0908958106;
RA Xu G., Shin S.B., Jaffrey S.R.;
RT "Global profiling of protease cleavage sites by chemoselective
RT labeling of protein N-termini.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-244, AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- CATALYTIC ACTIVITY: ATP + L-asparagine + tRNA(Asn) = AMP +
CC diphosphate + L-asparaginyl-tRNA(Asn).
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AJ000334; CAA04008.1; -; mRNA.
DR EMBL; D84273; BAA34600.1; -; mRNA.
DR EMBL; AK222835; BAD96555.1; -; mRNA.
DR EMBL; BC001687; AAH01687.1; -; mRNA.
DR RefSeq; NP_004530.1; NM_004539.3.
DR UniGene; Hs.465224; -.
DR ProteinModelPortal; O43776; -.
DR SMR; O43776; 115-548.
DR IntAct; O43776; 5.
DR MINT; MINT-5003848; -.
DR STRING; 9606.ENSP00000256854; -.
DR DrugBank; DB00174; L-Asparagine.
DR PhosphoSite; O43776; -.
DR PaxDb; O43776; -.
DR PeptideAtlas; O43776; -.
DR PRIDE; O43776; -.
DR Ensembl; ENST00000256854; ENSP00000256854; ENSG00000134440.
DR GeneID; 4677; -.
DR KEGG; hsa:4677; -.
DR UCSC; uc002lgs.2; human.
DR CTD; 4677; -.
DR GeneCards; GC18M055267; -.
DR HGNC; HGNC:7643; NARS.
DR HPA; CAB034257; -.
DR HPA; HPA040017; -.
DR MIM; 108410; gene.
DR neXtProt; NX_O43776; -.
DR PharmGKB; PA31447; -.
DR eggNOG; COG0017; -.
DR HOGENOM; HOG000226035; -.
DR HOVERGEN; HBG059844; -.
DR InParanoid; O43776; -.
DR KO; K01893; -.
DR OMA; AVYGMLN; -.
DR OrthoDB; EOG7KQ21G; -.
DR PhylomeDB; O43776; -.
DR BRENDA; 6.1.1.22; 2681.
DR Reactome; REACT_71; Gene Expression.
DR ChiTaRS; NARS; human.
DR GeneWiki; NARS_(gene); -.
DR GenomeRNAi; 4677; -.
DR NextBio; 18028; -.
DR PRO; PR:O43776; -.
DR ArrayExpress; O43776; -.
DR Bgee; O43776; -.
DR CleanEx; HS_ASNS; -.
DR CleanEx; HS_NARS; -.
DR Genevestigator; O43776; -.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR GO; GO:0004816; F:asparagine-tRNA ligase activity; TAS:ProtInc.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006418; P:tRNA aminoacylation for protein translation; TAS:Reactome.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR004364; aa-tRNA-synt_II.
DR InterPro; IPR018150; aa-tRNA-synt_II-like.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR004522; Asn-tRNA-ligase.
DR InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR PANTHER; PTHR22594; PTHR22594; 1.
DR PANTHER; PTHR22594:SF16; PTHR22594:SF16; 1.
DR Pfam; PF00152; tRNA-synt_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PRINTS; PR01042; TRNASYNTHASP.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00457; asnS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 1: Evidence at protein level;
KW Acetylation; Aminoacyl-tRNA synthetase; ATP-binding;
KW Complete proteome; Cytoplasm; Direct protein sequencing; Ligase;
KW Nucleotide-binding; Phosphoprotein; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1 548 Asparagine--tRNA ligase, cytoplasmic.
FT /FTId=PRO_0000176496.
FT MOD_RES 61 61 Phosphoserine.
FT MOD_RES 244 244 N6-acetyllysine.
FT CONFLICT 391 391 M -> V (in Ref. 3; BAD96555).
SQ SEQUENCE 548 AA; 62943 MW; CDD442C4E962CDDB CRC64;
MVLAELYVSD REGSDATGDG TKEKPFKTGL KALMTVGKEP FPTIYVDSQK ENERWNVISK
SQLKNIKKMW HREQMKSESR EKKEAEDSLR REKNLEEAKK ITIKNDPSLP EPKCVKIGAL
EGYRGQRVKV FGWVHRLRRQ GKNLMFLVLR DGTGYLQCVL ADELCQCYNG VLLSTESSVA
VYGMLNLTPK GKQAPGGHEL SCDFWELIGL APAGGADNLI NEESDVDVQL NNRHMMIRGE
NMSKILKARS MVTRCFRDHF FDRGYYEVTP PTLVQTQVEG GATLFKLDYF GEEAFLTQSS
QLYLETCLPA LGDVFCIAQS YRAEQSRTRR HLAEYTHVEA ECPFLTFDDL LNRLEDLVCD
VVDRILKSPA GSIVHELNPN FQPPKRPFKR MNYSDAIVWL KEHDVKKEDG TFYEFGEDIP
EAPERLMTDT INEPILLCRF PVEIKSFYMQ RCPEDSRLTE SVDVLMPNVG EIVGGSMRIF
DSEEILAGYK REGIDPTPYY WYTDQRKYGT CPHGGYGLGL ERFLTWILNR YHIRDVCLYP
RFVQRCTP
//
ID SYNC_HUMAN Reviewed; 548 AA.
AC O43776; Q53GU6;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-JUN-1998, sequence version 1.
DT 22-JAN-2014, entry version 123.
DE RecName: Full=Asparagine--tRNA ligase, cytoplasmic;
DE EC=6.1.1.22;
DE AltName: Full=Asparaginyl-tRNA synthetase;
DE Short=AsnRS;
GN Name=NARS;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Liver;
RX PubMed=9421509; DOI=10.1093/nar/26.2.521;
RA Beaulande M., Tarbouriech N., Haertlein M.;
RT "Human cytosolic asparaginyl-tRNA synthetase: cDNA sequence,
RT functional expression in Escherichia coli and characterization as
RT human autoantigen.";
RL Nucleic Acids Res. 26:521-524(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9801298; DOI=10.1093/nar/26.22.5045;
RA Shiba K., Motegi H., Yoshida M., Noda T.;
RT "Human asparaginyl-tRNA synthetase: molecular cloning and the
RT inference of the evolutionary history of Asx-tRNA synthetase family.";
RL Nucleic Acids Res. 26:5045-5051(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 2-12.
RC TISSUE=Leukemic T-cell;
RX PubMed=19892738; DOI=10.1073/pnas.0908958106;
RA Xu G., Shin S.B., Jaffrey S.R.;
RT "Global profiling of protease cleavage sites by chemoselective
RT labeling of protein N-termini.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-244, AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- CATALYTIC ACTIVITY: ATP + L-asparagine + tRNA(Asn) = AMP +
CC diphosphate + L-asparaginyl-tRNA(Asn).
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AJ000334; CAA04008.1; -; mRNA.
DR EMBL; D84273; BAA34600.1; -; mRNA.
DR EMBL; AK222835; BAD96555.1; -; mRNA.
DR EMBL; BC001687; AAH01687.1; -; mRNA.
DR RefSeq; NP_004530.1; NM_004539.3.
DR UniGene; Hs.465224; -.
DR ProteinModelPortal; O43776; -.
DR SMR; O43776; 115-548.
DR IntAct; O43776; 5.
DR MINT; MINT-5003848; -.
DR STRING; 9606.ENSP00000256854; -.
DR DrugBank; DB00174; L-Asparagine.
DR PhosphoSite; O43776; -.
DR PaxDb; O43776; -.
DR PeptideAtlas; O43776; -.
DR PRIDE; O43776; -.
DR Ensembl; ENST00000256854; ENSP00000256854; ENSG00000134440.
DR GeneID; 4677; -.
DR KEGG; hsa:4677; -.
DR UCSC; uc002lgs.2; human.
DR CTD; 4677; -.
DR GeneCards; GC18M055267; -.
DR HGNC; HGNC:7643; NARS.
DR HPA; CAB034257; -.
DR HPA; HPA040017; -.
DR MIM; 108410; gene.
DR neXtProt; NX_O43776; -.
DR PharmGKB; PA31447; -.
DR eggNOG; COG0017; -.
DR HOGENOM; HOG000226035; -.
DR HOVERGEN; HBG059844; -.
DR InParanoid; O43776; -.
DR KO; K01893; -.
DR OMA; AVYGMLN; -.
DR OrthoDB; EOG7KQ21G; -.
DR PhylomeDB; O43776; -.
DR BRENDA; 6.1.1.22; 2681.
DR Reactome; REACT_71; Gene Expression.
DR ChiTaRS; NARS; human.
DR GeneWiki; NARS_(gene); -.
DR GenomeRNAi; 4677; -.
DR NextBio; 18028; -.
DR PRO; PR:O43776; -.
DR ArrayExpress; O43776; -.
DR Bgee; O43776; -.
DR CleanEx; HS_ASNS; -.
DR CleanEx; HS_NARS; -.
DR Genevestigator; O43776; -.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR GO; GO:0004816; F:asparagine-tRNA ligase activity; TAS:ProtInc.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006418; P:tRNA aminoacylation for protein translation; TAS:Reactome.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR004364; aa-tRNA-synt_II.
DR InterPro; IPR018150; aa-tRNA-synt_II-like.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR004522; Asn-tRNA-ligase.
DR InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR PANTHER; PTHR22594; PTHR22594; 1.
DR PANTHER; PTHR22594:SF16; PTHR22594:SF16; 1.
DR Pfam; PF00152; tRNA-synt_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PRINTS; PR01042; TRNASYNTHASP.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00457; asnS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 1: Evidence at protein level;
KW Acetylation; Aminoacyl-tRNA synthetase; ATP-binding;
KW Complete proteome; Cytoplasm; Direct protein sequencing; Ligase;
KW Nucleotide-binding; Phosphoprotein; Protein biosynthesis;
KW Reference proteome.
FT CHAIN 1 548 Asparagine--tRNA ligase, cytoplasmic.
FT /FTId=PRO_0000176496.
FT MOD_RES 61 61 Phosphoserine.
FT MOD_RES 244 244 N6-acetyllysine.
FT CONFLICT 391 391 M -> V (in Ref. 3; BAD96555).
SQ SEQUENCE 548 AA; 62943 MW; CDD442C4E962CDDB CRC64;
MVLAELYVSD REGSDATGDG TKEKPFKTGL KALMTVGKEP FPTIYVDSQK ENERWNVISK
SQLKNIKKMW HREQMKSESR EKKEAEDSLR REKNLEEAKK ITIKNDPSLP EPKCVKIGAL
EGYRGQRVKV FGWVHRLRRQ GKNLMFLVLR DGTGYLQCVL ADELCQCYNG VLLSTESSVA
VYGMLNLTPK GKQAPGGHEL SCDFWELIGL APAGGADNLI NEESDVDVQL NNRHMMIRGE
NMSKILKARS MVTRCFRDHF FDRGYYEVTP PTLVQTQVEG GATLFKLDYF GEEAFLTQSS
QLYLETCLPA LGDVFCIAQS YRAEQSRTRR HLAEYTHVEA ECPFLTFDDL LNRLEDLVCD
VVDRILKSPA GSIVHELNPN FQPPKRPFKR MNYSDAIVWL KEHDVKKEDG TFYEFGEDIP
EAPERLMTDT INEPILLCRF PVEIKSFYMQ RCPEDSRLTE SVDVLMPNVG EIVGGSMRIF
DSEEILAGYK REGIDPTPYY WYTDQRKYGT CPHGGYGLGL ERFLTWILNR YHIRDVCLYP
RFVQRCTP
//
MIM
108410
*RECORD*
*FIELD* NO
108410
*FIELD* TI
*108410 ASPARAGINYL-tRNA SYNTHETASE; NARS
;;ASNRS
*FIELD* TX
CLONING
Cirullo et al. (1983) isolated hybrids between human peripheral
read moreleukocytes and a temperature-sensitive CHO cell line with a thermolabile
asparaginyl-tRNA synthetase (EC 6.1.1.22), which they symbolized 'asnS.'
Hybrids selected at 39 degrees C required the presence of human
chromosome 18. Temperature-resistant hybrid cells contained 2 forms of
ASNRS: 1 highly thermal resistant, like the human enzyme, and 1 highly
thermolabile, like the CHO mutant enzyme.
Beaulande et al. (1998) cloned a human cytosolic ASNRS cDNA from a liver
cDNA library. The deduced 548-amino acid protein has a predicted
molecular mass of 62.9 kD and shares 53% sequence identity with the S.
cerevisiae homolog.
MAPPING
Using a DNA probe in human-rodent hybrid cells, Shows (1983) found that
asparaginyl-tRNA synthetase segregated with peptidase A, a chromosome 18
marker.
GENE FUNCTION
By Western blot analysis, Beaulande et al. (1998) showed that a human
autoimmune serum (anti-KS) neutralized human AsnRSc activity, suggesting
that it is a class II aminoacyl-tRNA synthetase involved in autoimmune
reactions.
*FIELD* RF
1. Beaulande, M.; Tarbouriech, N.; Hartlein, M.: Human cytosolic
asparaginyl-tRNA synthetase: cDNA sequence, functional expression
in Escherichia coli and characterization as human autoantigen. Nucleic
Acids Res. 26: 521-524, 1998.
2. Cirullo, R. E.; Arredondo-Vega, F. X.; Smith, M.; Wasmuth, J. J.
: Isolation and characterization of interspecific heat-resistant hybrids
between a temperature-sensitive Chinese hamster cell asparaginyl-tRNA
synthetase mutant and normal human leukocytes: assignment of human
asnS gene to chromosome 18. Somat. Cell Genet. 9: 215-233, 1983.
3. Shows, T. B.: Personal Communication. Buffalo, N. Y. 1/11/1983.
*FIELD* CN
Carol A. Bocchini - updated: 10/27/2009
*FIELD* CD
Victor A. McKusick: 6/4/1986
*FIELD* ED
carol: 10/27/2009
alopez: 7/29/1998
warfield: 4/7/1994
supermim: 3/16/1992
carol: 11/12/1990
supermim: 3/20/1990
ddp: 10/26/1989
marie: 3/25/1988
*RECORD*
*FIELD* NO
108410
*FIELD* TI
*108410 ASPARAGINYL-tRNA SYNTHETASE; NARS
;;ASNRS
*FIELD* TX
CLONING
Cirullo et al. (1983) isolated hybrids between human peripheral
read moreleukocytes and a temperature-sensitive CHO cell line with a thermolabile
asparaginyl-tRNA synthetase (EC 6.1.1.22), which they symbolized 'asnS.'
Hybrids selected at 39 degrees C required the presence of human
chromosome 18. Temperature-resistant hybrid cells contained 2 forms of
ASNRS: 1 highly thermal resistant, like the human enzyme, and 1 highly
thermolabile, like the CHO mutant enzyme.
Beaulande et al. (1998) cloned a human cytosolic ASNRS cDNA from a liver
cDNA library. The deduced 548-amino acid protein has a predicted
molecular mass of 62.9 kD and shares 53% sequence identity with the S.
cerevisiae homolog.
MAPPING
Using a DNA probe in human-rodent hybrid cells, Shows (1983) found that
asparaginyl-tRNA synthetase segregated with peptidase A, a chromosome 18
marker.
GENE FUNCTION
By Western blot analysis, Beaulande et al. (1998) showed that a human
autoimmune serum (anti-KS) neutralized human AsnRSc activity, suggesting
that it is a class II aminoacyl-tRNA synthetase involved in autoimmune
reactions.
*FIELD* RF
1. Beaulande, M.; Tarbouriech, N.; Hartlein, M.: Human cytosolic
asparaginyl-tRNA synthetase: cDNA sequence, functional expression
in Escherichia coli and characterization as human autoantigen. Nucleic
Acids Res. 26: 521-524, 1998.
2. Cirullo, R. E.; Arredondo-Vega, F. X.; Smith, M.; Wasmuth, J. J.
: Isolation and characterization of interspecific heat-resistant hybrids
between a temperature-sensitive Chinese hamster cell asparaginyl-tRNA
synthetase mutant and normal human leukocytes: assignment of human
asnS gene to chromosome 18. Somat. Cell Genet. 9: 215-233, 1983.
3. Shows, T. B.: Personal Communication. Buffalo, N. Y. 1/11/1983.
*FIELD* CN
Carol A. Bocchini - updated: 10/27/2009
*FIELD* CD
Victor A. McKusick: 6/4/1986
*FIELD* ED
carol: 10/27/2009
alopez: 7/29/1998
warfield: 4/7/1994
supermim: 3/16/1992
carol: 11/12/1990
supermim: 3/20/1990
ddp: 10/26/1989
marie: 3/25/1988