Full text data of GTF2A2
GTF2A2
(TF2A2)
[Confidence: low (only semi-automatic identification from reviews)]
Transcription initiation factor IIA subunit 2 (General transcription factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; Transcription initiation factor IIA gamma chain; TFIIA-gamma)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Transcription initiation factor IIA subunit 2 (General transcription factor IIA subunit 2; TFIIA p12 subunit; TFIIA-12; TFIIAS; Transcription initiation factor IIA gamma chain; TFIIA-gamma)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
P52657
ID T2AG_HUMAN Reviewed; 109 AA.
AC P52657; A8MYQ7; Q6FGB5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-1996, sequence version 1.
DT 22-JAN-2014, entry version 125.
DE RecName: Full=Transcription initiation factor IIA subunit 2;
DE AltName: Full=General transcription factor IIA subunit 2;
DE AltName: Full=TFIIA p12 subunit;
DE Short=TFIIA-12;
DE Short=TFIIAS;
DE AltName: Full=Transcription initiation factor IIA gamma chain;
DE Short=TFIIA-gamma;
GN Name=GTF2A2; Synonyms=TF2A2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7958900;
RA Sun X., Ma D., Sheldon M., Yeung K., Reinberg D.;
RT "Reconstitution of human TFIIA activity from recombinant polypeptides:
RT a role in TFIID-mediated transcription.";
RL Genes Dev. 8:2336-2348(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7958899;
RA Ozer J., Moore P.A., Bolden A.H., Lee A., Rosen C.A., Lieberman P.M.;
RT "Molecular cloning of the small (gamma) subunit of human TFIIA reveals
RT functions critical for activated transcription.";
RL Genes Dev. 8:2324-2335(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7724559; DOI=10.1073/pnas.92.8.3313;
RA Dejong J., Bernstein R., Roeder R.G.;
RT "Human general transcription factor TFIIA: characterization of a cDNA
RT encoding the small subunit and requirement for basal and activated
RT transcription.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3313-3317(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R.,
RA Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G.,
RA Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A.,
RA Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W.,
RA Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X.,
RA Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K.,
RA Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S.,
RA Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human
RT chromosome 15.";
RL Nature 440:671-675(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP INTERACTION WITH SV40 LARGE T ANTIGEN.
RX PubMed=9632777;
RA Damania B., Lieberman P., Alwine J.C.;
RT "Simian virus 40 large T antigen stabilizes the TATA-binding protein-
RT TFIIA complex on the TATA element.";
RL Mol. Cell. Biol. 18:3926-3935(1998).
RN [10]
RP INTERACTION WITH NCOA6.
RX PubMed=10567404; DOI=10.1074/jbc.274.48.34283;
RA Lee S.-K., Anzick S.L., Choi J.-E., Bubendorf L., Guan X.-Y.,
RA Jung Y.-K., Kallioniemi O.-P., Kononen J., Trent J.M., Azorsa D.,
RA Jhun B.-H., Cheong J.H., Lee Y.C., Meltzer P.S., Lee J.W.;
RT "A nuclear factor ASC-2, as a cancer-amplified transcriptional
RT coactivator essential for ligand-dependent transactivation by nuclear
RT receptors in vivo.";
RL J. Biol. Chem. 274:34283-34293(1999).
RN [11]
RP FUNCTION, SUBUNIT, AND IDENTIFICATION IN A COMPLEX WITH GTF2A1 AND
RP TBP.
RX PubMed=11030333; DOI=10.1016/S1097-2765(00)00052-6;
RA Mitsiou D.J., Stunnenberg H.G.;
RT "TAC, a TBP-sans-TAFs complex containing the unprocessed
RT TFIIAalphabeta precursor and the TFIIAgamma subunit.";
RL Mol. Cell 6:527-537(2000).
CC -!- FUNCTION: TFIIA is a component of the transcription machinery of
CC RNA polymerase II and plays an important role in transcriptional
CC activation. TFIIA in a complex with TBP mediates transcriptional
CC activity.
CC -!- SUBUNIT: TFIIA is a heterodimer of the large unprocessed subunit 1
CC and a small subunit gamma. It was originally believed to be a
CC heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit
CC (p12). Interacts with NCOA6 general coactivator (By similarity).
CC TFIIA forms a complex with TBP. Interacts with SV40 Large T
CC antigen.
CC -!- INTERACTION:
CC P20226:TBP; NbExp=2; IntAct=EBI-1045262, EBI-355371;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TFIIA subunit 2 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; X81713; CAA57357.1; -; mRNA.
DR EMBL; U14193; AAA64951.1; -; mRNA.
DR EMBL; U21242; AAB58247.1; -; mRNA.
DR EMBL; BT007362; AAP36026.1; -; mRNA.
DR EMBL; CR542192; CAG46989.1; -; mRNA.
DR EMBL; AC092755; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471082; EAW77573.1; -; Genomic_DNA.
DR EMBL; BC000287; AAH00287.1; -; mRNA.
DR EMBL; BC001919; AAH01919.1; -; mRNA.
DR PIR; I38952; I38952.
DR RefSeq; NP_004483.1; NM_004492.2.
DR RefSeq; XP_005254381.1; XM_005254324.1.
DR RefSeq; XP_005254382.1; XM_005254325.1.
DR UniGene; Hs.512934; -.
DR PDB; 1NVP; X-ray; 2.10 A; D=2-109.
DR PDBsum; 1NVP; -.
DR ProteinModelPortal; P52657; -.
DR SMR; P52657; 3-99.
DR IntAct; P52657; 2.
DR STRING; 9606.ENSP00000379372; -.
DR BindingDB; P52657; -.
DR PhosphoSite; P52657; -.
DR DMDM; 1729806; -.
DR PaxDb; P52657; -.
DR PRIDE; P52657; -.
DR DNASU; 2958; -.
DR Ensembl; ENST00000396060; ENSP00000379372; ENSG00000140307.
DR Ensembl; ENST00000396061; ENSP00000379373; ENSG00000140307.
DR Ensembl; ENST00000396063; ENSP00000379375; ENSG00000140307.
DR GeneID; 2958; -.
DR KEGG; hsa:2958; -.
DR UCSC; uc002agg.3; human.
DR CTD; 2958; -.
DR GeneCards; GC15M059930; -.
DR HGNC; HGNC:4647; GTF2A2.
DR MIM; 600519; gene.
DR neXtProt; NX_P52657; -.
DR PharmGKB; PA29034; -.
DR eggNOG; COG5123; -.
DR HOGENOM; HOG000167251; -.
DR HOVERGEN; HBG000826; -.
DR InParanoid; P52657; -.
DR KO; K03123; -.
DR OMA; WTFIVKN; -.
DR OrthoDB; EOG7RFTKP; -.
DR PhylomeDB; P52657; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_1788; Transcription.
DR Reactome; REACT_71; Gene Expression.
DR EvolutionaryTrace; P52657; -.
DR GenomeRNAi; 2958; -.
DR NextBio; 11726; -.
DR PRO; PR:P52657; -.
DR ArrayExpress; P52657; -.
DR Bgee; P52657; -.
DR CleanEx; HS_GTF2A2; -.
DR Genevestigator; P52657; -.
DR GO; GO:0005672; C:transcription factor TFIIA complex; IDA:BHF-UCL.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:BHF-UCL.
DR GO; GO:0019048; P:modulation by virus of host morphology or physiology; IEA:UniProtKB-KW.
DR GO; GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
DR GO; GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
DR GO; GO:0051123; P:RNA polymerase II transcriptional preinitiation complex assembly; IDA:BHF-UCL.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
DR GO; GO:0016032; P:viral process; TAS:Reactome.
DR Gene3D; 1.10.287.190; -; 1.
DR Gene3D; 2.30.18.10; -; 1.
DR InterPro; IPR009083; TFIIA_a-hlx.
DR InterPro; IPR009088; TFIIA_b-brl.
DR InterPro; IPR003194; TFIIA_gsu.
DR InterPro; IPR015871; TFIIA_gsu_C.
DR InterPro; IPR015872; TFIIA_gsu_N.
DR PANTHER; PTHR10966; PTHR10966; 1.
DR Pfam; PF02751; TFIIA_gamma_C; 1.
DR Pfam; PF02268; TFIIA_gamma_N; 1.
DR PIRSF; PIRSF009415; Hum_TFIIA_gamma; 1.
DR ProDom; PD009224; TFIIA_gsu_N; 1.
DR SUPFAM; SSF47396; SSF47396; 1.
DR SUPFAM; SSF50784; SSF50784; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Host-virus interaction; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1 109 Transcription initiation factor IIA
FT subunit 2.
FT /FTId=PRO_0000194042.
FT HELIX 4 7
FT HELIX 10 24
FT HELIX 30 50
FT STRAND 55 68
FT STRAND 71 82
FT STRAND 87 98
SQ SEQUENCE 109 AA; 12457 MW; 938E7DC8B9982AE8 CRC64;
MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG
SLNTYRFCDN VWTFVLNDVE FREVTELIKV DKVKIVACDG KNTGSNTTE
//
ID T2AG_HUMAN Reviewed; 109 AA.
AC P52657; A8MYQ7; Q6FGB5;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-1996, sequence version 1.
DT 22-JAN-2014, entry version 125.
DE RecName: Full=Transcription initiation factor IIA subunit 2;
DE AltName: Full=General transcription factor IIA subunit 2;
DE AltName: Full=TFIIA p12 subunit;
DE Short=TFIIA-12;
DE Short=TFIIAS;
DE AltName: Full=Transcription initiation factor IIA gamma chain;
DE Short=TFIIA-gamma;
GN Name=GTF2A2; Synonyms=TF2A2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7958900;
RA Sun X., Ma D., Sheldon M., Yeung K., Reinberg D.;
RT "Reconstitution of human TFIIA activity from recombinant polypeptides:
RT a role in TFIID-mediated transcription.";
RL Genes Dev. 8:2336-2348(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7958899;
RA Ozer J., Moore P.A., Bolden A.H., Lee A., Rosen C.A., Lieberman P.M.;
RT "Molecular cloning of the small (gamma) subunit of human TFIIA reveals
RT functions critical for activated transcription.";
RL Genes Dev. 8:2324-2335(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7724559; DOI=10.1073/pnas.92.8.3313;
RA Dejong J., Bernstein R., Roeder R.G.;
RT "Human general transcription factor TFIIA: characterization of a cDNA
RT encoding the small subunit and requirement for basal and activated
RT transcription.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3313-3317(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R.,
RA Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G.,
RA Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A.,
RA Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W.,
RA Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X.,
RA Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K.,
RA Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S.,
RA Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human
RT chromosome 15.";
RL Nature 440:671-675(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP INTERACTION WITH SV40 LARGE T ANTIGEN.
RX PubMed=9632777;
RA Damania B., Lieberman P., Alwine J.C.;
RT "Simian virus 40 large T antigen stabilizes the TATA-binding protein-
RT TFIIA complex on the TATA element.";
RL Mol. Cell. Biol. 18:3926-3935(1998).
RN [10]
RP INTERACTION WITH NCOA6.
RX PubMed=10567404; DOI=10.1074/jbc.274.48.34283;
RA Lee S.-K., Anzick S.L., Choi J.-E., Bubendorf L., Guan X.-Y.,
RA Jung Y.-K., Kallioniemi O.-P., Kononen J., Trent J.M., Azorsa D.,
RA Jhun B.-H., Cheong J.H., Lee Y.C., Meltzer P.S., Lee J.W.;
RT "A nuclear factor ASC-2, as a cancer-amplified transcriptional
RT coactivator essential for ligand-dependent transactivation by nuclear
RT receptors in vivo.";
RL J. Biol. Chem. 274:34283-34293(1999).
RN [11]
RP FUNCTION, SUBUNIT, AND IDENTIFICATION IN A COMPLEX WITH GTF2A1 AND
RP TBP.
RX PubMed=11030333; DOI=10.1016/S1097-2765(00)00052-6;
RA Mitsiou D.J., Stunnenberg H.G.;
RT "TAC, a TBP-sans-TAFs complex containing the unprocessed
RT TFIIAalphabeta precursor and the TFIIAgamma subunit.";
RL Mol. Cell 6:527-537(2000).
CC -!- FUNCTION: TFIIA is a component of the transcription machinery of
CC RNA polymerase II and plays an important role in transcriptional
CC activation. TFIIA in a complex with TBP mediates transcriptional
CC activity.
CC -!- SUBUNIT: TFIIA is a heterodimer of the large unprocessed subunit 1
CC and a small subunit gamma. It was originally believed to be a
CC heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit
CC (p12). Interacts with NCOA6 general coactivator (By similarity).
CC TFIIA forms a complex with TBP. Interacts with SV40 Large T
CC antigen.
CC -!- INTERACTION:
CC P20226:TBP; NbExp=2; IntAct=EBI-1045262, EBI-355371;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TFIIA subunit 2 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; X81713; CAA57357.1; -; mRNA.
DR EMBL; U14193; AAA64951.1; -; mRNA.
DR EMBL; U21242; AAB58247.1; -; mRNA.
DR EMBL; BT007362; AAP36026.1; -; mRNA.
DR EMBL; CR542192; CAG46989.1; -; mRNA.
DR EMBL; AC092755; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471082; EAW77573.1; -; Genomic_DNA.
DR EMBL; BC000287; AAH00287.1; -; mRNA.
DR EMBL; BC001919; AAH01919.1; -; mRNA.
DR PIR; I38952; I38952.
DR RefSeq; NP_004483.1; NM_004492.2.
DR RefSeq; XP_005254381.1; XM_005254324.1.
DR RefSeq; XP_005254382.1; XM_005254325.1.
DR UniGene; Hs.512934; -.
DR PDB; 1NVP; X-ray; 2.10 A; D=2-109.
DR PDBsum; 1NVP; -.
DR ProteinModelPortal; P52657; -.
DR SMR; P52657; 3-99.
DR IntAct; P52657; 2.
DR STRING; 9606.ENSP00000379372; -.
DR BindingDB; P52657; -.
DR PhosphoSite; P52657; -.
DR DMDM; 1729806; -.
DR PaxDb; P52657; -.
DR PRIDE; P52657; -.
DR DNASU; 2958; -.
DR Ensembl; ENST00000396060; ENSP00000379372; ENSG00000140307.
DR Ensembl; ENST00000396061; ENSP00000379373; ENSG00000140307.
DR Ensembl; ENST00000396063; ENSP00000379375; ENSG00000140307.
DR GeneID; 2958; -.
DR KEGG; hsa:2958; -.
DR UCSC; uc002agg.3; human.
DR CTD; 2958; -.
DR GeneCards; GC15M059930; -.
DR HGNC; HGNC:4647; GTF2A2.
DR MIM; 600519; gene.
DR neXtProt; NX_P52657; -.
DR PharmGKB; PA29034; -.
DR eggNOG; COG5123; -.
DR HOGENOM; HOG000167251; -.
DR HOVERGEN; HBG000826; -.
DR InParanoid; P52657; -.
DR KO; K03123; -.
DR OMA; WTFIVKN; -.
DR OrthoDB; EOG7RFTKP; -.
DR PhylomeDB; P52657; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_1788; Transcription.
DR Reactome; REACT_71; Gene Expression.
DR EvolutionaryTrace; P52657; -.
DR GenomeRNAi; 2958; -.
DR NextBio; 11726; -.
DR PRO; PR:P52657; -.
DR ArrayExpress; P52657; -.
DR Bgee; P52657; -.
DR CleanEx; HS_GTF2A2; -.
DR Genevestigator; P52657; -.
DR GO; GO:0005672; C:transcription factor TFIIA complex; IDA:BHF-UCL.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:BHF-UCL.
DR GO; GO:0019048; P:modulation by virus of host morphology or physiology; IEA:UniProtKB-KW.
DR GO; GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
DR GO; GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
DR GO; GO:0051123; P:RNA polymerase II transcriptional preinitiation complex assembly; IDA:BHF-UCL.
DR GO; GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
DR GO; GO:0016032; P:viral process; TAS:Reactome.
DR Gene3D; 1.10.287.190; -; 1.
DR Gene3D; 2.30.18.10; -; 1.
DR InterPro; IPR009083; TFIIA_a-hlx.
DR InterPro; IPR009088; TFIIA_b-brl.
DR InterPro; IPR003194; TFIIA_gsu.
DR InterPro; IPR015871; TFIIA_gsu_C.
DR InterPro; IPR015872; TFIIA_gsu_N.
DR PANTHER; PTHR10966; PTHR10966; 1.
DR Pfam; PF02751; TFIIA_gamma_C; 1.
DR Pfam; PF02268; TFIIA_gamma_N; 1.
DR PIRSF; PIRSF009415; Hum_TFIIA_gamma; 1.
DR ProDom; PD009224; TFIIA_gsu_N; 1.
DR SUPFAM; SSF47396; SSF47396; 1.
DR SUPFAM; SSF50784; SSF50784; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Host-virus interaction; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1 109 Transcription initiation factor IIA
FT subunit 2.
FT /FTId=PRO_0000194042.
FT HELIX 4 7
FT HELIX 10 24
FT HELIX 30 50
FT STRAND 55 68
FT STRAND 71 82
FT STRAND 87 98
SQ SEQUENCE 109 AA; 12457 MW; 938E7DC8B9982AE8 CRC64;
MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG
SLNTYRFCDN VWTFVLNDVE FREVTELIKV DKVKIVACDG KNTGSNTTE
//
MIM
600519
*RECORD*
*FIELD* NO
600519
*FIELD* TI
*600519 GENERAL TRANSCRIPTION FACTOR IIA, GAMMA SUBUNIT; GTF2A2
;;TRANSCRIPTION FACTOR IIA, 2; TF2A2
read more*FIELD* TX
DESCRIPTION
Accurate transcription initiation on TATA-containing class II genes
involves the ordered assembly of RNA polymerase II (POLR2A; 180660) and
the general initiation factors TFIIA, TFIIB (189963), TFIID (313650),
TFIIE (189962), TFIIF (189968), TFIIG/TFIIJ, and TFIIH (189972). The
first step involves recognition of the TATA element by the TATA-binding
subunit (TBP; 600075) and may be regulated by TFIIA, a factor that
interacts with both TBP and a TBP-associated factor (TAF; 600475) in
TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in
higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha
subunit and a 19-kD beta subunit encoded by the N- and C-terminal
regions of GTF2A1 (600520), respectively, and a 12-kD gamma subunit
encoded by GTF2A2 (DeJong et al., 1995).
CLONING
DeJong et al. (1995) isolated a cDNA clone encoding the 12-kD TFIIA
gamma subunit.
GENE FUNCTION
High levels of gene transcription by RNA polymerase II depend on high
rates of transcription initiation and reinitiation. Initiation requires
recruitment of the complete transcription machinery to a promoter, a
process facilitated by activators and chromatin remodeling factors.
Reinitiation is thought to occur through a different pathway. After
initiation, a subset of the transcription machinery remains at the
promoter, forming a platform for assembly of a second transcription
complex. Yudkovsky et al. (2000) described the isolation of a
reinitiation intermediate in yeast that includes transcription factors
TFIID, TFIIA, TFIIH, TFIIE, and Mediator (see 602984). This intermediate
can act as a scaffold for formation of a functional reinitiation
complex. Formation of this scaffold is dependent on ATP and TFIIH. In
yeast, the scaffold is stabilized in the presence of the activator
Gal4-VP16, but not Gal4-AH, suggesting a new role for some activators
and Mediator in promoting high levels of transcription.
GENE STRUCTURE
Upadhyaya et al. (2002) determined that the GTF2A2 gene contains 5 exons
and spans 19 kb.
MAPPING
By genomic sequence analysis, Upadhyaya et al. (2002) mapped the GTF2A2
gene to chromosome 15q22. They identified putative GTF2A2 pseudogenes on
chromosomes 1, 8, and 9.
*FIELD* RF
1. DeJong, J.; Bernstein, R.; Roeder, R. G.: Human general transcription
factor TFIIA: characterization of a cDNA encoding the small subunit
and requirement for basal and activated transcription. Proc. Nat.
Acad. Sci. 92: 3313-3317, 1995.
2. Upadhyaya, A. B.; Khan, M.; Mou, T.-C.; Junker, M.; Gray, D. M.;
DeJong, J.: The germ cell-specific transcription factor ALF: structural
properties and stabilization of the Tata-binding protein (TBP)-DNA
complex. J. Biol. Chem. 277: 34208-34216, 2002.
3. Yudkovsky, N.; Ranish, J. A.; Hahn, S.: A transcription reinitiation
intermediate that is stabilized by activator. Nature 408: 225-229,
2000.
*FIELD* CN
Patricia A. Hartz - updated: 8/21/2006
Ada Hamosh - updated: 11/8/2000
*FIELD* CD
Victor A. McKusick: 5/7/1995
*FIELD* ED
alopez: 07/02/2010
terry: 7/1/2010
mgross: 8/28/2006
terry: 8/21/2006
alopez: 11/8/2000
mark: 7/2/1997
mark: 5/8/1995
mark: 5/7/1995
*RECORD*
*FIELD* NO
600519
*FIELD* TI
*600519 GENERAL TRANSCRIPTION FACTOR IIA, GAMMA SUBUNIT; GTF2A2
;;TRANSCRIPTION FACTOR IIA, 2; TF2A2
read more*FIELD* TX
DESCRIPTION
Accurate transcription initiation on TATA-containing class II genes
involves the ordered assembly of RNA polymerase II (POLR2A; 180660) and
the general initiation factors TFIIA, TFIIB (189963), TFIID (313650),
TFIIE (189962), TFIIF (189968), TFIIG/TFIIJ, and TFIIH (189972). The
first step involves recognition of the TATA element by the TATA-binding
subunit (TBP; 600075) and may be regulated by TFIIA, a factor that
interacts with both TBP and a TBP-associated factor (TAF; 600475) in
TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in
higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha
subunit and a 19-kD beta subunit encoded by the N- and C-terminal
regions of GTF2A1 (600520), respectively, and a 12-kD gamma subunit
encoded by GTF2A2 (DeJong et al., 1995).
CLONING
DeJong et al. (1995) isolated a cDNA clone encoding the 12-kD TFIIA
gamma subunit.
GENE FUNCTION
High levels of gene transcription by RNA polymerase II depend on high
rates of transcription initiation and reinitiation. Initiation requires
recruitment of the complete transcription machinery to a promoter, a
process facilitated by activators and chromatin remodeling factors.
Reinitiation is thought to occur through a different pathway. After
initiation, a subset of the transcription machinery remains at the
promoter, forming a platform for assembly of a second transcription
complex. Yudkovsky et al. (2000) described the isolation of a
reinitiation intermediate in yeast that includes transcription factors
TFIID, TFIIA, TFIIH, TFIIE, and Mediator (see 602984). This intermediate
can act as a scaffold for formation of a functional reinitiation
complex. Formation of this scaffold is dependent on ATP and TFIIH. In
yeast, the scaffold is stabilized in the presence of the activator
Gal4-VP16, but not Gal4-AH, suggesting a new role for some activators
and Mediator in promoting high levels of transcription.
GENE STRUCTURE
Upadhyaya et al. (2002) determined that the GTF2A2 gene contains 5 exons
and spans 19 kb.
MAPPING
By genomic sequence analysis, Upadhyaya et al. (2002) mapped the GTF2A2
gene to chromosome 15q22. They identified putative GTF2A2 pseudogenes on
chromosomes 1, 8, and 9.
*FIELD* RF
1. DeJong, J.; Bernstein, R.; Roeder, R. G.: Human general transcription
factor TFIIA: characterization of a cDNA encoding the small subunit
and requirement for basal and activated transcription. Proc. Nat.
Acad. Sci. 92: 3313-3317, 1995.
2. Upadhyaya, A. B.; Khan, M.; Mou, T.-C.; Junker, M.; Gray, D. M.;
DeJong, J.: The germ cell-specific transcription factor ALF: structural
properties and stabilization of the Tata-binding protein (TBP)-DNA
complex. J. Biol. Chem. 277: 34208-34216, 2002.
3. Yudkovsky, N.; Ranish, J. A.; Hahn, S.: A transcription reinitiation
intermediate that is stabilized by activator. Nature 408: 225-229,
2000.
*FIELD* CN
Patricia A. Hartz - updated: 8/21/2006
Ada Hamosh - updated: 11/8/2000
*FIELD* CD
Victor A. McKusick: 5/7/1995
*FIELD* ED
alopez: 07/02/2010
terry: 7/1/2010
mgross: 8/28/2006
terry: 8/21/2006
alopez: 11/8/2000
mark: 7/2/1997
mark: 5/8/1995
mark: 5/7/1995