Full text data of TMED5
TMED5
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Transmembrane emp24 domain-containing protein 5 (p24 family protein gamma-2; p24gamma2; p28; Flags: Precursor)
Transmembrane emp24 domain-containing protein 5 (p24 family protein gamma-2; p24gamma2; p28; Flags: Precursor)
UniProt
Q9Y3A6
ID TMED5_HUMAN Reviewed; 229 AA.
AC Q9Y3A6; B1AKT4; B2R703; D3DT38; Q96AX8;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1999, sequence version 1.
DT 22-JAN-2014, entry version 118.
DE RecName: Full=Transmembrane emp24 domain-containing protein 5;
DE AltName: Full=p24 family protein gamma-2;
DE Short=p24gamma2;
DE AltName: Full=p28;
DE Flags: Precursor;
GN Name=TMED5; ORFNames=CGI-100, UNQ397/PRO733;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ILE-175.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Placenta;
RA Li W.B., Gruber C., Jessee J., Polayes D.;
RT "Full-length cDNA libraries and normalization.";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ILE-175.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ILE-175.
RC TISSUE=Bone marrow, Brain, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH TMED9 AND TMED10.
RX PubMed=19948005; DOI=10.1111/j.1600-0854.2009.01009.x;
RA Koegler E., Bonnon C., Waldmeier L., Mitrovic S., Halbeisen R.,
RA Hauri H.P.;
RT "p28, a novel ERGIC/cis Golgi protein, required for Golgi ribbon
RT formation.";
RL Traffic 11:70-89(2010).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Potential role in vesicular protein trafficking, mainly
CC in the early secretory pathway. Required for the maintenance of
CC the Golgi apparatus; involved in protein exchange between Golgi
CC stacks during assembly. Probably not required for COPI-vesicle-
CC mediated retrograde transport.
CC -!- SUBUNIT: Interacts with TMED9 and TMED10.
CC -!- INTERACTION:
CC Q96LA8:PRMT6; NbExp=2; IntAct=EBI-7560959, EBI-912440;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC type I membrane protein. Golgi apparatus, cis-Golgi network
CC membrane; Single-pass type I membrane protein. Endoplasmic
CC reticulum-Golgi intermediate compartment membrane; Single-pass
CC type I membrane protein. Note=Probably cycles between compartments
CC of the early secretatory pathway.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y3A6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y3A6-2; Sequence=VSP_046365;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the EMP24/GP25L family.
CC -!- SIMILARITY: Contains 1 GOLD domain.
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DR EMBL; AF151858; AAD34095.1; -; mRNA.
DR EMBL; AY359039; AAQ89398.1; -; mRNA.
DR EMBL; BX379269; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK312787; BAG35650.1; -; mRNA.
DR EMBL; AL117354; CAI23500.1; -; Genomic_DNA.
DR EMBL; AC126124; CAI23500.1; JOINED; Genomic_DNA.
DR EMBL; AL117354; CAI23501.1; -; Genomic_DNA.
DR EMBL; AC126124; CAI23501.1; JOINED; Genomic_DNA.
DR EMBL; CH471097; EAW73081.1; -; Genomic_DNA.
DR EMBL; CH471097; EAW73082.1; -; Genomic_DNA.
DR EMBL; BC016365; AAH16365.1; -; mRNA.
DR EMBL; BC016556; AAH16556.1; -; mRNA.
DR EMBL; BC070051; AAH70051.1; -; mRNA.
DR RefSeq; NP_001161302.1; NM_001167830.1.
DR RefSeq; NP_057124.3; NM_016040.4.
DR UniGene; Hs.482873; -.
DR ProteinModelPortal; Q9Y3A6; -.
DR IntAct; Q9Y3A6; 2.
DR MINT; MINT-3085589; -.
DR STRING; 9606.ENSP00000359305; -.
DR PhosphoSite; Q9Y3A6; -.
DR DMDM; 12585534; -.
DR PaxDb; Q9Y3A6; -.
DR PRIDE; Q9Y3A6; -.
DR DNASU; 50999; -.
DR Ensembl; ENST00000370282; ENSP00000359305; ENSG00000117500.
DR Ensembl; ENST00000479918; ENSP00000418992; ENSG00000117500.
DR GeneID; 50999; -.
DR KEGG; hsa:50999; -.
DR UCSC; uc001dpo.3; human.
DR CTD; 50999; -.
DR GeneCards; GC01M093615; -.
DR HGNC; HGNC:24251; TMED5.
DR HPA; HPA050289; -.
DR neXtProt; NX_Q9Y3A6; -.
DR PharmGKB; PA134942067; -.
DR eggNOG; NOG284261; -.
DR HOGENOM; HOG000039809; -.
DR HOVERGEN; HBG000271; -.
DR InParanoid; Q9Y3A6; -.
DR KO; K14825; -.
DR OMA; EEGDYMF; -.
DR OrthoDB; EOG7CG716; -.
DR PhylomeDB; Q9Y3A6; -.
DR GenomeRNAi; 50999; -.
DR NextBio; 53448; -.
DR PRO; PR:Q9Y3A6; -.
DR ArrayExpress; Q9Y3A6; -.
DR Bgee; Q9Y3A6; -.
DR CleanEx; HS_TMED5; -.
DR Genevestigator; Q9Y3A6; -.
DR GO; GO:0005801; C:cis-Golgi network; IDA:UniProtKB.
DR GO; GO:0070971; C:endoplasmic reticulum exit site; IDA:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0090161; P:Golgi ribbon formation; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR009038; GOLD.
DR Pfam; PF01105; EMP24_GP25L; 1.
DR SUPFAM; SSF101576; SSF101576; 1.
DR PROSITE; PS50866; GOLD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Complete proteome; Endoplasmic reticulum;
KW Golgi apparatus; Membrane; Polymorphism; Protein transport;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1 27 Potential.
FT CHAIN 28 229 Transmembrane emp24 domain-containing
FT protein 5.
FT /FTId=PRO_0000010389.
FT TOPO_DOM 28 196 Lumenal (Potential).
FT TRANSMEM 197 217 Helical; (Potential).
FT TOPO_DOM 218 229 Cytoplasmic (Potential).
FT DOMAIN 45 126 GOLD.
FT VAR_SEQ 158 229 ESINSIKSRLSKSGHIQTLLRAFEARDRNIQESNFDRVNFW
FT SMVNLVVMVVVSAIQVYMLKSLFEDKRKSRT -> DPIIKI
FT ASDVQIRLHLGIHQQHQVQTKQKWAHTNSA (in
FT isoform 2).
FT /FTId=VSP_046365.
FT VARIANT 175 175 T -> I (in dbSNP:rs1060622).
FT /FTId=VAR_017150.
SQ SEQUENCE 229 AA; 26005 MW; 6973E0DACBF15B19 CRC64;
MGDKIWLPFP VLLLAALPPV LLPGAAGFTP SLDSDFTFTL PAGQKECFYQ PMPLKASLEI
EYQVLDGAGL DIDFHLASPE GKTLVFEQRK SDGVHTVETE VGDYMFCFDN TFSTISEKVI
FFELILDNMG EQAQEQEDWK KYITGTDILD MKLEDILESI NSIKSRLSKS GHIQTLLRAF
EARDRNIQES NFDRVNFWSM VNLVVMVVVS AIQVYMLKSL FEDKRKSRT
//
ID TMED5_HUMAN Reviewed; 229 AA.
AC Q9Y3A6; B1AKT4; B2R703; D3DT38; Q96AX8;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1999, sequence version 1.
DT 22-JAN-2014, entry version 118.
DE RecName: Full=Transmembrane emp24 domain-containing protein 5;
DE AltName: Full=p24 family protein gamma-2;
DE Short=p24gamma2;
DE AltName: Full=p28;
DE Flags: Precursor;
GN Name=TMED5; ORFNames=CGI-100, UNQ397/PRO733;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ILE-175.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Placenta;
RA Li W.B., Gruber C., Jessee J., Polayes D.;
RT "Full-length cDNA libraries and normalization.";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ILE-175.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT
RP ILE-175.
RC TISSUE=Bone marrow, Brain, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH TMED9 AND TMED10.
RX PubMed=19948005; DOI=10.1111/j.1600-0854.2009.01009.x;
RA Koegler E., Bonnon C., Waldmeier L., Mitrovic S., Halbeisen R.,
RA Hauri H.P.;
RT "p28, a novel ERGIC/cis Golgi protein, required for Golgi ribbon
RT formation.";
RL Traffic 11:70-89(2010).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Potential role in vesicular protein trafficking, mainly
CC in the early secretory pathway. Required for the maintenance of
CC the Golgi apparatus; involved in protein exchange between Golgi
CC stacks during assembly. Probably not required for COPI-vesicle-
CC mediated retrograde transport.
CC -!- SUBUNIT: Interacts with TMED9 and TMED10.
CC -!- INTERACTION:
CC Q96LA8:PRMT6; NbExp=2; IntAct=EBI-7560959, EBI-912440;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC type I membrane protein. Golgi apparatus, cis-Golgi network
CC membrane; Single-pass type I membrane protein. Endoplasmic
CC reticulum-Golgi intermediate compartment membrane; Single-pass
CC type I membrane protein. Note=Probably cycles between compartments
CC of the early secretatory pathway.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9Y3A6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y3A6-2; Sequence=VSP_046365;
CC Note=No experimental confirmation available;
CC -!- SIMILARITY: Belongs to the EMP24/GP25L family.
CC -!- SIMILARITY: Contains 1 GOLD domain.
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DR EMBL; AF151858; AAD34095.1; -; mRNA.
DR EMBL; AY359039; AAQ89398.1; -; mRNA.
DR EMBL; BX379269; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK312787; BAG35650.1; -; mRNA.
DR EMBL; AL117354; CAI23500.1; -; Genomic_DNA.
DR EMBL; AC126124; CAI23500.1; JOINED; Genomic_DNA.
DR EMBL; AL117354; CAI23501.1; -; Genomic_DNA.
DR EMBL; AC126124; CAI23501.1; JOINED; Genomic_DNA.
DR EMBL; CH471097; EAW73081.1; -; Genomic_DNA.
DR EMBL; CH471097; EAW73082.1; -; Genomic_DNA.
DR EMBL; BC016365; AAH16365.1; -; mRNA.
DR EMBL; BC016556; AAH16556.1; -; mRNA.
DR EMBL; BC070051; AAH70051.1; -; mRNA.
DR RefSeq; NP_001161302.1; NM_001167830.1.
DR RefSeq; NP_057124.3; NM_016040.4.
DR UniGene; Hs.482873; -.
DR ProteinModelPortal; Q9Y3A6; -.
DR IntAct; Q9Y3A6; 2.
DR MINT; MINT-3085589; -.
DR STRING; 9606.ENSP00000359305; -.
DR PhosphoSite; Q9Y3A6; -.
DR DMDM; 12585534; -.
DR PaxDb; Q9Y3A6; -.
DR PRIDE; Q9Y3A6; -.
DR DNASU; 50999; -.
DR Ensembl; ENST00000370282; ENSP00000359305; ENSG00000117500.
DR Ensembl; ENST00000479918; ENSP00000418992; ENSG00000117500.
DR GeneID; 50999; -.
DR KEGG; hsa:50999; -.
DR UCSC; uc001dpo.3; human.
DR CTD; 50999; -.
DR GeneCards; GC01M093615; -.
DR HGNC; HGNC:24251; TMED5.
DR HPA; HPA050289; -.
DR neXtProt; NX_Q9Y3A6; -.
DR PharmGKB; PA134942067; -.
DR eggNOG; NOG284261; -.
DR HOGENOM; HOG000039809; -.
DR HOVERGEN; HBG000271; -.
DR InParanoid; Q9Y3A6; -.
DR KO; K14825; -.
DR OMA; EEGDYMF; -.
DR OrthoDB; EOG7CG716; -.
DR PhylomeDB; Q9Y3A6; -.
DR GenomeRNAi; 50999; -.
DR NextBio; 53448; -.
DR PRO; PR:Q9Y3A6; -.
DR ArrayExpress; Q9Y3A6; -.
DR Bgee; Q9Y3A6; -.
DR CleanEx; HS_TMED5; -.
DR Genevestigator; Q9Y3A6; -.
DR GO; GO:0005801; C:cis-Golgi network; IDA:UniProtKB.
DR GO; GO:0070971; C:endoplasmic reticulum exit site; IDA:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
DR GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0090161; P:Golgi ribbon formation; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR009038; GOLD.
DR Pfam; PF01105; EMP24_GP25L; 1.
DR SUPFAM; SSF101576; SSF101576; 1.
DR PROSITE; PS50866; GOLD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Complete proteome; Endoplasmic reticulum;
KW Golgi apparatus; Membrane; Polymorphism; Protein transport;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1 27 Potential.
FT CHAIN 28 229 Transmembrane emp24 domain-containing
FT protein 5.
FT /FTId=PRO_0000010389.
FT TOPO_DOM 28 196 Lumenal (Potential).
FT TRANSMEM 197 217 Helical; (Potential).
FT TOPO_DOM 218 229 Cytoplasmic (Potential).
FT DOMAIN 45 126 GOLD.
FT VAR_SEQ 158 229 ESINSIKSRLSKSGHIQTLLRAFEARDRNIQESNFDRVNFW
FT SMVNLVVMVVVSAIQVYMLKSLFEDKRKSRT -> DPIIKI
FT ASDVQIRLHLGIHQQHQVQTKQKWAHTNSA (in
FT isoform 2).
FT /FTId=VSP_046365.
FT VARIANT 175 175 T -> I (in dbSNP:rs1060622).
FT /FTId=VAR_017150.
SQ SEQUENCE 229 AA; 26005 MW; 6973E0DACBF15B19 CRC64;
MGDKIWLPFP VLLLAALPPV LLPGAAGFTP SLDSDFTFTL PAGQKECFYQ PMPLKASLEI
EYQVLDGAGL DIDFHLASPE GKTLVFEQRK SDGVHTVETE VGDYMFCFDN TFSTISEKVI
FFELILDNMG EQAQEQEDWK KYITGTDILD MKLEDILESI NSIKSRLSKS GHIQTLLRAF
EARDRNIQES NFDRVNFWSM VNLVVMVVVS AIQVYMLKSL FEDKRKSRT
//