Full text data of VAMP3
VAMP3
(SYB3)
[Confidence: high (present in two of the MS resources)]
Vesicle-associated membrane protein 3; VAMP-3 (Cellubrevin; CEB; Synaptobrevin-3)
Vesicle-associated membrane protein 3; VAMP-3 (Cellubrevin; CEB; Synaptobrevin-3)
UniProt
Q15836
ID VAMP3_HUMAN Reviewed; 100 AA.
AC Q15836;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 3.
DT 22-JAN-2014, entry version 128.
DE RecName: Full=Vesicle-associated membrane protein 3;
DE Short=VAMP-3;
DE AltName: Full=Cellubrevin;
DE Short=CEB;
DE AltName: Full=Synaptobrevin-3;
GN Name=VAMP3; Synonyms=SYB3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9885218;
RA Bernstein A.M., Whiteheart S.W.;
RT "Identification of a cellubrevin/vesicle associated membrane protein 3
RT homologue in human platelets.";
RL Blood 93:571-579(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 2-14.
RC TISSUE=Platelet;
RX PubMed=12665801; DOI=10.1038/nbt810;
RA Gevaert K., Goethals M., Martens L., Van Damme J., Staes A.,
RA Thomas G.R., Vandekerckhove J.;
RT "Exploring proteomes and analyzing protein processing by mass
RT spectrometric identification of sorted N-terminal peptides.";
RL Nat. Biotechnol. 21:566-569(2003).
RN [6]
RP FUNCTION.
RX PubMed=18195106; DOI=10.1083/jcb.200707136;
RA Ganley I.G., Espinosa E., Pfeffer S.R.;
RT "A syntaxin 10-SNARE complex distinguishes two distinct transport
RT routes from endosomes to the trans-Golgi in human cells.";
RL J. Cell Biol. 180:159-172(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: SNARE involved in vesicular transport from the late
CC endosomes to the trans-Golgi network.
CC -!- SUBUNIT: Interacts with BVES (via the C-terminus cytoplasmic tail)
CC (By similarity).
CC -!- INTERACTION:
CC O55012:Picalm (xeno); NbExp=2; IntAct=EBI-722343, EBI-915601;
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type IV membrane
CC protein (Probable). Cell junction, synapse, synaptosome (By
CC similarity).
CC -!- SIMILARITY: Belongs to the synaptobrevin family.
CC -!- SIMILARITY: Contains 1 v-SNARE coiled-coil homology domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; U64520; AAB05814.1; -; mRNA.
DR EMBL; BT007327; AAP35991.1; -; mRNA.
DR EMBL; Z98884; CAB63146.1; -; Genomic_DNA.
DR EMBL; BC003570; AAH03570.1; -; mRNA.
DR EMBL; BC007050; AAH07050.1; -; mRNA.
DR RefSeq; NP_004772.1; NM_004781.3.
DR UniGene; Hs.66708; -.
DR ProteinModelPortal; Q15836; -.
DR SMR; Q15836; 11-75.
DR IntAct; Q15836; 9.
DR STRING; 9606.ENSP00000054666; -.
DR PhosphoSite; Q15836; -.
DR DMDM; 2501082; -.
DR PaxDb; Q15836; -.
DR PRIDE; Q15836; -.
DR DNASU; 9341; -.
DR Ensembl; ENST00000054666; ENSP00000054666; ENSG00000049245.
DR GeneID; 9341; -.
DR KEGG; hsa:9341; -.
DR UCSC; uc001aol.3; human.
DR CTD; 9341; -.
DR GeneCards; GC01P007765; -.
DR HGNC; HGNC:12644; VAMP3.
DR MIM; 603657; gene.
DR neXtProt; NX_Q15836; -.
DR PharmGKB; PA37268; -.
DR eggNOG; NOG266115; -.
DR HOGENOM; HOG000042711; -.
DR HOVERGEN; HBG006675; -.
DR InParanoid; Q15836; -.
DR KO; K13505; -.
DR OMA; FTHACAF; -.
DR OrthoDB; EOG7MSMRJ; -.
DR PhylomeDB; Q15836; -.
DR ChiTaRS; VAMP3; human.
DR GeneWiki; VAMP3; -.
DR GenomeRNAi; 9341; -.
DR NextBio; 34987; -.
DR PMAP-CutDB; Q15836; -.
DR PRO; PR:Q15836; -.
DR ArrayExpress; Q15836; -.
DR Bgee; Q15836; -.
DR CleanEx; HS_VAMP3; -.
DR Genevestigator; Q15836; -.
DR GO; GO:0016324; C:apical plasma membrane; IEA:Ensembl.
DR GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW.
DR GO; GO:0030136; C:clathrin-coated vesicle; IDA:UniProtKB.
DR GO; GO:0030665; C:clathrin-coated vesicle membrane; IEA:Ensembl.
DR GO; GO:0016021; C:integral to membrane; TAS:ProtInc.
DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0055037; C:recycling endosome; IDA:MGI.
DR GO; GO:0030141; C:secretory granule; IEA:Ensembl.
DR GO; GO:0031201; C:SNARE complex; IEA:Ensembl.
DR GO; GO:0045202; C:synapse; IEA:UniProtKB-KW.
DR GO; GO:0017156; P:calcium ion-dependent exocytosis; IEA:Ensembl.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IEA:Ensembl.
DR GO; GO:0061025; P:membrane fusion; TAS:ProtInc.
DR GO; GO:0001921; P:positive regulation of receptor recycling; ISS:UniProtKB.
DR GO; GO:0006461; P:protein complex assembly; TAS:ProtInc.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IDA:UniProtKB.
DR GO; GO:0034446; P:substrate adhesion-dependent cell spreading; ISS:UniProtKB.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; TAS:ProtInc.
DR InterPro; IPR001388; Synaptobrevin.
DR InterPro; IPR016444; Synaptobrevin_met/fun.
DR Pfam; PF00957; Synaptobrevin; 1.
DR PIRSF; PIRSF005409; Synaptobrevin_euk; 1.
DR PRINTS; PR00219; SYNAPTOBREVN.
DR PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR PROSITE; PS50892; V_SNARE; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell junction; Coiled coil; Complete proteome;
KW Direct protein sequencing; Membrane; Protein transport;
KW Reference proteome; Synapse; Synaptosome; Transmembrane;
KW Transmembrane helix; Transport.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 100 Vesicle-associated membrane protein 3.
FT /FTId=PRO_0000206728.
FT TOPO_DOM 2 77 Cytoplasmic (Potential).
FT TRANSMEM 78 98 Helical; Anchor for type IV membrane
FT protein; (Potential).
FT TOPO_DOM 99 100 Vesicular (Potential).
FT DOMAIN 14 74 v-SNARE coiled-coil homology.
FT MOD_RES 2 2 N-acetylserine.
SQ SEQUENCE 100 AA; 11309 MW; 347E9163157AFF42 CRC64;
MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA DALQAGASQF
ETSAAKLKRK YWWKNCKMWA IGITVLVIFI IIIIVWVVSS
//
ID VAMP3_HUMAN Reviewed; 100 AA.
AC Q15836;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 3.
DT 22-JAN-2014, entry version 128.
DE RecName: Full=Vesicle-associated membrane protein 3;
DE Short=VAMP-3;
DE AltName: Full=Cellubrevin;
DE Short=CEB;
DE AltName: Full=Synaptobrevin-3;
GN Name=VAMP3; Synonyms=SYB3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9885218;
RA Bernstein A.M., Whiteheart S.W.;
RT "Identification of a cellubrevin/vesicle associated membrane protein 3
RT homologue in human platelets.";
RL Blood 93:571-579(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D.,
RA Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A.,
RA Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F.,
RA McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C.,
RA Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K.,
RA Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G.,
RA Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D.,
RA Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G.,
RA Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J.,
RA Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R.,
RA Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D.,
RA Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G.,
RA Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M.,
RA Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J.,
RA Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M.,
RA Loveland J., Lovell J., Lush M.J., Lyne R., Martin S.,
RA Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S.,
RA Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C.,
RA Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z.,
RA Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E.,
RA Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A.,
RA Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R.,
RA Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V.,
RA Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 2-14.
RC TISSUE=Platelet;
RX PubMed=12665801; DOI=10.1038/nbt810;
RA Gevaert K., Goethals M., Martens L., Van Damme J., Staes A.,
RA Thomas G.R., Vandekerckhove J.;
RT "Exploring proteomes and analyzing protein processing by mass
RT spectrometric identification of sorted N-terminal peptides.";
RL Nat. Biotechnol. 21:566-569(2003).
RN [6]
RP FUNCTION.
RX PubMed=18195106; DOI=10.1083/jcb.200707136;
RA Ganley I.G., Espinosa E., Pfeffer S.R.;
RT "A syntaxin 10-SNARE complex distinguishes two distinct transport
RT routes from endosomes to the trans-Golgi in human cells.";
RL J. Cell Biol. 180:159-172(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY, AND
RP CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: SNARE involved in vesicular transport from the late
CC endosomes to the trans-Golgi network.
CC -!- SUBUNIT: Interacts with BVES (via the C-terminus cytoplasmic tail)
CC (By similarity).
CC -!- INTERACTION:
CC O55012:Picalm (xeno); NbExp=2; IntAct=EBI-722343, EBI-915601;
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type IV membrane
CC protein (Probable). Cell junction, synapse, synaptosome (By
CC similarity).
CC -!- SIMILARITY: Belongs to the synaptobrevin family.
CC -!- SIMILARITY: Contains 1 v-SNARE coiled-coil homology domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; U64520; AAB05814.1; -; mRNA.
DR EMBL; BT007327; AAP35991.1; -; mRNA.
DR EMBL; Z98884; CAB63146.1; -; Genomic_DNA.
DR EMBL; BC003570; AAH03570.1; -; mRNA.
DR EMBL; BC007050; AAH07050.1; -; mRNA.
DR RefSeq; NP_004772.1; NM_004781.3.
DR UniGene; Hs.66708; -.
DR ProteinModelPortal; Q15836; -.
DR SMR; Q15836; 11-75.
DR IntAct; Q15836; 9.
DR STRING; 9606.ENSP00000054666; -.
DR PhosphoSite; Q15836; -.
DR DMDM; 2501082; -.
DR PaxDb; Q15836; -.
DR PRIDE; Q15836; -.
DR DNASU; 9341; -.
DR Ensembl; ENST00000054666; ENSP00000054666; ENSG00000049245.
DR GeneID; 9341; -.
DR KEGG; hsa:9341; -.
DR UCSC; uc001aol.3; human.
DR CTD; 9341; -.
DR GeneCards; GC01P007765; -.
DR HGNC; HGNC:12644; VAMP3.
DR MIM; 603657; gene.
DR neXtProt; NX_Q15836; -.
DR PharmGKB; PA37268; -.
DR eggNOG; NOG266115; -.
DR HOGENOM; HOG000042711; -.
DR HOVERGEN; HBG006675; -.
DR InParanoid; Q15836; -.
DR KO; K13505; -.
DR OMA; FTHACAF; -.
DR OrthoDB; EOG7MSMRJ; -.
DR PhylomeDB; Q15836; -.
DR ChiTaRS; VAMP3; human.
DR GeneWiki; VAMP3; -.
DR GenomeRNAi; 9341; -.
DR NextBio; 34987; -.
DR PMAP-CutDB; Q15836; -.
DR PRO; PR:Q15836; -.
DR ArrayExpress; Q15836; -.
DR Bgee; Q15836; -.
DR CleanEx; HS_VAMP3; -.
DR Genevestigator; Q15836; -.
DR GO; GO:0016324; C:apical plasma membrane; IEA:Ensembl.
DR GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW.
DR GO; GO:0030136; C:clathrin-coated vesicle; IDA:UniProtKB.
DR GO; GO:0030665; C:clathrin-coated vesicle membrane; IEA:Ensembl.
DR GO; GO:0016021; C:integral to membrane; TAS:ProtInc.
DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0055037; C:recycling endosome; IDA:MGI.
DR GO; GO:0030141; C:secretory granule; IEA:Ensembl.
DR GO; GO:0031201; C:SNARE complex; IEA:Ensembl.
DR GO; GO:0045202; C:synapse; IEA:UniProtKB-KW.
DR GO; GO:0017156; P:calcium ion-dependent exocytosis; IEA:Ensembl.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IEA:Ensembl.
DR GO; GO:0061025; P:membrane fusion; TAS:ProtInc.
DR GO; GO:0001921; P:positive regulation of receptor recycling; ISS:UniProtKB.
DR GO; GO:0006461; P:protein complex assembly; TAS:ProtInc.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IDA:UniProtKB.
DR GO; GO:0034446; P:substrate adhesion-dependent cell spreading; ISS:UniProtKB.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; TAS:ProtInc.
DR InterPro; IPR001388; Synaptobrevin.
DR InterPro; IPR016444; Synaptobrevin_met/fun.
DR Pfam; PF00957; Synaptobrevin; 1.
DR PIRSF; PIRSF005409; Synaptobrevin_euk; 1.
DR PRINTS; PR00219; SYNAPTOBREVN.
DR PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR PROSITE; PS50892; V_SNARE; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell junction; Coiled coil; Complete proteome;
KW Direct protein sequencing; Membrane; Protein transport;
KW Reference proteome; Synapse; Synaptosome; Transmembrane;
KW Transmembrane helix; Transport.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 100 Vesicle-associated membrane protein 3.
FT /FTId=PRO_0000206728.
FT TOPO_DOM 2 77 Cytoplasmic (Potential).
FT TRANSMEM 78 98 Helical; Anchor for type IV membrane
FT protein; (Potential).
FT TOPO_DOM 99 100 Vesicular (Potential).
FT DOMAIN 14 74 v-SNARE coiled-coil homology.
FT MOD_RES 2 2 N-acetylserine.
SQ SEQUENCE 100 AA; 11309 MW; 347E9163157AFF42 CRC64;
MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA DALQAGASQF
ETSAAKLKRK YWWKNCKMWA IGITVLVIFI IIIIVWVVSS
//
MIM
603657
*RECORD*
*FIELD* NO
603657
*FIELD* TI
*603657 VESICLE-ASSOCIATED MEMBRANE PROTEIN 3; VAMP3
;;CELLUBREVIN; CEB
*FIELD* TX
read more
CLONING
To understand more about the membrane trafficking events in platelets
that have been activated, Bernstein and Whiteheart (1999) searched for
proteins that could mediate platelet exocytosis or endocytosis. They
identified a novel member of the vesicle-associated membrane protein
(VAMP)/synaptobrevin family. See VAMP8 (603177). Originally cloned from
a human megakaryocyte library, the VAMP3 gene was found by Northern blot
analysis to be expressed in many human tissues. Because of its high
homology to other known VAMPs, its broad tissue distribution, and its
subcellular localization, this protein appeared to be the human
equivalent of the rodent cellubrevin (CEB). Bernstein and Whiteheart
(1999) showed that in platelets CEB resides on a compartment that is not
mobilized to the plasma membrane on calcium or thrombin stimulation.
GENE FUNCTION
Membrane traffic in activated macrophages is required for 2 critical
events in innate immunity: proinflammatory cytokine secretion and
phagocytosis of pathogens. Murray et al. (2005) found a joint
trafficking pathway linking both actions, which may economize membrane
transport and augment the immune response. Tumor necrosis factor-alpha
(TNFA; 191160) is trafficked from the Golgi to the recycling endosome,
where VAMP3 mediates its delivery to the cell surface at the site of
phagocytic cup formation. Fusion of the recycling endosome at the cup
simultaneously allows rapid release of TNF-alpha and expands the
membrane for phagocytosis.
MAPPING
In the study of a form of autosomal recessive early-onset parkinsonism,
PARK7 (606324), and search for candidate genes in the region of mapping
on 1p36, van Duijn et al. (2001) found that the VAMP3 gene is in this
region by scanning a working draft of the Human Genome Project's
sequence.
*FIELD* RF
1. Bernstein, A. M.; Whiteheart, S. W.: Identification of a cellubrevin/vesicle
associated membrane protein 3 homologue in human platelets. Blood 93:
571-579, 1999.
2. Murray, R. Z.; Kay, J. G.; Sangermani, D. G.; Stow, J. L.: A role
for the phagosome in cytokine secretion. Science 310: 1492-1495,
2005.
3. van Duijn, C. M.; Dekker, M. C. J.; Bonifati, V.; Galjaard, R.
J.; Houwing-Duistermaat, J. J.; Snijders, P. J. L. M.; Testers, L.;
Breedveld, G. J.; Horstink, M.; Sandkuijl, L. A.; van Swieten, J.
C.; Oostra, B. A.; Heutink, P.: PARK7, a novel locus for autosomal
recessive early-onset parkinsonism, on chromosome 1p36. Am. J. Hum.
Genet. 69: 629-634, 2001.
*FIELD* CN
Ada Hamosh - updated: 1/11/2006
Victor A. McKusick - updated: 9/27/2001
*FIELD* CD
Victor A. McKusick: 3/18/1999
*FIELD* ED
alopez: 01/12/2006
terry: 1/11/2006
alopez: 9/28/2001
terry: 9/27/2001
carol: 7/26/1999
carol: 3/18/1999
*RECORD*
*FIELD* NO
603657
*FIELD* TI
*603657 VESICLE-ASSOCIATED MEMBRANE PROTEIN 3; VAMP3
;;CELLUBREVIN; CEB
*FIELD* TX
read more
CLONING
To understand more about the membrane trafficking events in platelets
that have been activated, Bernstein and Whiteheart (1999) searched for
proteins that could mediate platelet exocytosis or endocytosis. They
identified a novel member of the vesicle-associated membrane protein
(VAMP)/synaptobrevin family. See VAMP8 (603177). Originally cloned from
a human megakaryocyte library, the VAMP3 gene was found by Northern blot
analysis to be expressed in many human tissues. Because of its high
homology to other known VAMPs, its broad tissue distribution, and its
subcellular localization, this protein appeared to be the human
equivalent of the rodent cellubrevin (CEB). Bernstein and Whiteheart
(1999) showed that in platelets CEB resides on a compartment that is not
mobilized to the plasma membrane on calcium or thrombin stimulation.
GENE FUNCTION
Membrane traffic in activated macrophages is required for 2 critical
events in innate immunity: proinflammatory cytokine secretion and
phagocytosis of pathogens. Murray et al. (2005) found a joint
trafficking pathway linking both actions, which may economize membrane
transport and augment the immune response. Tumor necrosis factor-alpha
(TNFA; 191160) is trafficked from the Golgi to the recycling endosome,
where VAMP3 mediates its delivery to the cell surface at the site of
phagocytic cup formation. Fusion of the recycling endosome at the cup
simultaneously allows rapid release of TNF-alpha and expands the
membrane for phagocytosis.
MAPPING
In the study of a form of autosomal recessive early-onset parkinsonism,
PARK7 (606324), and search for candidate genes in the region of mapping
on 1p36, van Duijn et al. (2001) found that the VAMP3 gene is in this
region by scanning a working draft of the Human Genome Project's
sequence.
*FIELD* RF
1. Bernstein, A. M.; Whiteheart, S. W.: Identification of a cellubrevin/vesicle
associated membrane protein 3 homologue in human platelets. Blood 93:
571-579, 1999.
2. Murray, R. Z.; Kay, J. G.; Sangermani, D. G.; Stow, J. L.: A role
for the phagosome in cytokine secretion. Science 310: 1492-1495,
2005.
3. van Duijn, C. M.; Dekker, M. C. J.; Bonifati, V.; Galjaard, R.
J.; Houwing-Duistermaat, J. J.; Snijders, P. J. L. M.; Testers, L.;
Breedveld, G. J.; Horstink, M.; Sandkuijl, L. A.; van Swieten, J.
C.; Oostra, B. A.; Heutink, P.: PARK7, a novel locus for autosomal
recessive early-onset parkinsonism, on chromosome 1p36. Am. J. Hum.
Genet. 69: 629-634, 2001.
*FIELD* CN
Ada Hamosh - updated: 1/11/2006
Victor A. McKusick - updated: 9/27/2001
*FIELD* CD
Victor A. McKusick: 3/18/1999
*FIELD* ED
alopez: 01/12/2006
terry: 1/11/2006
alopez: 9/28/2001
terry: 9/27/2001
carol: 7/26/1999
carol: 3/18/1999