RBCC

Full text data of VAT1

VAT1 [Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Synaptic vesicle membrane protein VAT-1 homolog; 1.-.-.-

UniProt Q99536
ID VAT1_HUMAN Reviewed; 393 AA.
AC Q99536; A8K345; Q13035; Q5BKZ7; Q96A39; Q9BUT8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
read moreDT 27-SEP-2004, sequence version 2.
DT 22-JAN-2014, entry version 111.
DE RecName: Full=Synaptic vesicle membrane protein VAT-1 homolog;
DE EC=1.-.-.-;
GN Name=VAT1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8938427;
RA Smith T.M., Lee M.K., Szabo C.I., Jerome N., McEuen M., Taylor M.,
RA Hood L., King M.-C.;
RT "Complete genomic sequence and analysis of 117 kb of human DNA
RT containing the gene BRCA1.";
RL Genome Res. 6:1029-1049(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, Ovary, Skin, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R.,
RA Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N.,
RA Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B.,
RA Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J.,
RA Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E.,
RA Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J.,
RA Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C.,
RA Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in
RT the human lineage.";
RL Nature 440:1045-1049(2006).
RN [7]
RP PROTEIN SEQUENCE OF 2-61; 64-82; 85-96; 103-128; 211-225; 256-271;
RP 282-295 AND 322-344, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT
RP SER-2, AND MASS SPECTROMETRY.
RC TISSUE=Ovarian carcinoma;
RA Bienvenut W.V.;
RL Submitted (JAN-2010) to UniProtKB.
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 82-393.
RX PubMed=7774926; DOI=10.1016/0888-7543(95)80133-7;
RA Friedman L.S., Ostermeyer E.A., Lynch E.D., Szabo C.I., Meza J.E.,
RA Anderson L.A., Dowd P., Lee M.K., Rowell S.E., Ellison J., Boyd J.,
RA King M.-C.;
RT "22 genes from chromosome 17q21: cloning, sequencing, and
RT characterization of mutations in breast cancer families and tumors.";
RL Genomics 25:256-263(1995).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12898150; DOI=10.1007/s00403-003-0421-8;
RA Koch J., Foekens J., Timmermans M., Fink W., Wirzbach A., Kramer M.D.,
RA Schaefer B.M.;
RT "Human VAT-1: a calcium-regulated activation marker of human
RT epithelial cells.";
RL Arch. Dermatol. Res. 295:203-210(2003).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH MFN1 AND MFN2.
RX PubMed=17105775; DOI=10.1242/jcs.03253;
RA Eura Y., Ishihara N., Oka T., Mihara K.;
RT "Identification of a novel protein that regulates mitochondrial fusion
RT by modulating mitofusin (Mfn) protein function.";
RL J. Cell Sci. 119:4913-4925(2006).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=16964243; DOI=10.1038/nbt1240;
RA Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT "A probability-based approach for high-throughput protein
RT phosphorylation analysis and site localization.";
RL Nat. Biotechnol. 24:1285-1292(2006).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [13]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, AND MASS SPECTROMETRY.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [14]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND INDUCTION BY
RP WOUNDING.
RX PubMed=19508442; DOI=10.1111/j.1365-2990.2009.00993.x;
RA Mertsch S., Becker M., Lichota A., Paulus W., Senner V.;
RT "Vesicle amine transport protein-1 (VAT-1) is upregulated in
RT glioblastomas and promotes migration.";
RL Neuropathol. Appl. Neurobiol. 35:342-352(2009).
RN [15]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [16]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Possesses ATPase activity (By similarity). Plays a part
CC in calcium-regulated keratinocyte activation in epidermal repair
CC mechanisms. Has no effect on cell proliferation. Negatively
CC regulates mitochondrial fusion in cooperation with mitofusin
CC proteins (MFN1-2).
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Mitochondrion outer membrane;
CC Peripheral membrane protein. Note=The majority is localized in the
CC cytoplasm and a small amount is associated with mitochondria (By
CC similarity).
CC -!- TISSUE SPECIFICITY: Expressed in brain. Also expressed in
CC glioblastoma cells.
CC -!- INDUCTION: Increased expression in glioblastomas and on wounding,
CC in basal keratinocytes. This expression is calcium ion-dependent.
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. Quinone oxidoreductase subfamily.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC37596.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
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DR EMBL; L78833; AAC37596.1; ALT_INIT; Genomic_DNA.
DR EMBL; BT007369; AAP36033.1; -; mRNA.
DR EMBL; AK290460; BAF83149.1; -; mRNA.
DR EMBL; AC055866; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC135721; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471152; EAW60917.1; -; Genomic_DNA.
DR EMBL; BC001913; AAH01913.2; -; mRNA.
DR EMBL; BC008725; AAH08725.2; -; mRNA.
DR EMBL; BC014279; AAH14279.1; -; mRNA.
DR EMBL; BC015041; AAH15041.1; -; mRNA.
DR EMBL; BC090858; AAH90858.1; -; mRNA.
DR EMBL; U18009; AAA95990.1; -; mRNA.
DR RefSeq; NP_006364.2; NM_006373.3.
DR UniGene; Hs.514199; -.
DR ProteinModelPortal; Q99536; -.
DR SMR; Q99536; 37-387.
DR IntAct; Q99536; 6.
DR MINT; MINT-4999872; -.
DR PhosphoSite; Q99536; -.
DR DMDM; 52788294; -.
DR PaxDb; Q99536; -.
DR PeptideAtlas; Q99536; -.
DR PRIDE; Q99536; -.
DR DNASU; 10493; -.
DR Ensembl; ENST00000355653; ENSP00000347872; ENSG00000108828.
DR GeneID; 10493; -.
DR KEGG; hsa:10493; -.
DR UCSC; uc002icm.1; human.
DR CTD; 10493; -.
DR GeneCards; GC17M041166; -.
DR HGNC; HGNC:16919; VAT1.
DR HPA; HPA045170; -.
DR MIM; 604631; gene.
DR neXtProt; NX_Q99536; -.
DR PharmGKB; PA38422; -.
DR eggNOG; COG0604; -.
DR HOGENOM; HOG000294681; -.
DR HOVERGEN; HBG002466; -.
DR InParanoid; Q99536; -.
DR KO; K00540; -.
DR OMA; FTPGMEG; -.
DR OrthoDB; EOG7VQJD4; -.
DR PhylomeDB; Q99536; -.
DR ChiTaRS; VAT1; human.
DR GeneWiki; VAT1; -.
DR GenomeRNAi; 10493; -.
DR NextBio; 39818; -.
DR PRO; PR:Q99536; -.
DR ArrayExpress; Q99536; -.
DR Bgee; Q99536; -.
DR CleanEx; HS_VAT1; -.
DR Genevestigator; Q99536; -.
DR GO; GO:0016021; C:integral to membrane; TAS:ProtInc.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0010637; P:negative regulation of mitochondrial fusion; IMP:UniProtKB.
DR Gene3D; 3.40.50.720; -; 1.
DR Gene3D; 3.90.180.10; -; 1.
DR InterPro; IPR013149; ADH_C.
DR InterPro; IPR013154; ADH_GroES-like.
DR InterPro; IPR002085; ADH_SF_Zn-type.
DR InterPro; IPR011032; GroES-like.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS.
DR PANTHER; PTHR11695; PTHR11695; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR SUPFAM; SSF50129; SSF50129; 1.
DR PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1.
PE 1: Evidence at protein level;
KW Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing;
KW Membrane; Mitochondrion; Mitochondrion outer membrane; Oxidoreductase;
KW Phosphoprotein; Reference proteome.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 393 Synaptic vesicle membrane protein VAT-1
FT homolog.
FT /FTId=PRO_0000160918.
FT MOD_RES 2 2 N-acetylserine.
FT MOD_RES 18 18 Phosphoserine.
FT CONFLICT 71 71 P -> A (in Ref. 1; AAC37596).
FT CONFLICT 82 86 RLRAC -> NSARG (in Ref. 8; AAA95990).
FT CONFLICT 207 208 QL -> HV (in Ref. 8; AAA95990).
FT CONFLICT 392 392 E -> Q (in Ref. 1; AAC37596).
FT CONFLICT 393 393 N -> S (in Ref. 8; AAA95990).
SQ SEQUENCE 393 AA; 41920 MW; 25933347742C2FE6 CRC64;
MSDEREVAEA ATGEDASSPP PKTEAASDPQ HPAASEGAAA AAASPPLLRC LVLTGFGGYD
KVKLQSRPAA PPAPGPGQLT LRLRACGLNF ADLMARQGLY DRLPPLPVTP GMEGAGVVIA
VGEGVSDRKA GDRVMVLNRS GMWQEEVTVP SVQTFLIPEA MTFEEAAALL VNYITAYMVL
FDFGNLQPGH SVLVHMAAGG VGMAAVQLCR TVENVTVFGT ASASKHEALK ENGVTHPIDY
HTTDYVDEIK KISPKGVDIV MDPLGGSDTA KGYNLLKPMG KVVTYGMANL LTGPKRNLMA
LARTWWNQFS VTALQLLQAN RAVCGFHLGY LDGEVELVSG VVARLLALYN QGHIKPHIDS
VWPFEKVADA MKQMQEKKNV GKVLLVPGPE KEN
//

MIM 604631
*RECORD*
*FIELD* NO
604631
*FIELD* TI
*604631 VESICLE AMINE TRANSPORT PROTEIN 1
;;VAT1
*FIELD* TX
Synaptic vesicles are responsible for regulating the storage and release
read moreof neurotransmitters in the nerve terminal. Using expression screening
of a marine ray (Torpedo) electric lobe library, Linial et al. (1989)
identified a cDNA encoding a 379-amino acid synaptic vesicle integral
membrane protein, which they termed VAT1. Northern blot analysis
revealed that a 5.8-kb transcript is expressed in electromotor neurons.
Western blot analysis determined expression of a 42-kD protein in the
electric organ that copurified with synaptic vesicles.

While attempting to identify the BRCA1 (113705) gene, Friedman et al.
(1995) cloned cDNAs for a number of genes on chromosome 17q21, including
one with significant homology to Torpedo VAT1. By random sequencing of 4
cosmids from a human chromosome 17-specific library, Smith et al. (1996)
identified the sequence of 2 complete genes within 117 kb of DNA
containing the BRCA1 gene: RHO7 (601555) and VAT1, an abundant membrane
protein of cholinergic synaptic vesicles. The coding sequence of VAT1
predicts a 301-amino acid peptide. The authors found that a CpG island
precedes the VAT1 gene, which contains 6 exons spanning 8.1 kb. They
determined the following order of genes in this region: cen--IFP35
(600735)--VAT1--RHO7--BRCA1--1A1-3B--tel.

*FIELD* RF
1. Friedman, L. S.; Ostermeyer, E. A.; Lynch, E. D.; Welcsh, P.; Szabo,
C. I.; Meza, J. E.; Anderson, L. A.; Dowd, P.; Lee, M. K.; Rowell,
S. E.; Ellison, J.; Boyd, J.; King, M.-C.: 22 genes from chromosome
17q21: cloning, sequencing, and characterization of mutations in breast
cancer families and tumors. Genomics 25: 256-163, 1995.

2. Linial, M.; Miller, K.; Scheller, R. H.: VAT-1: an abundant membrane
protein from Torpedo cholinergic synaptic vesicles. Neuron 2: 1265-1273,
1989.

3. Smith, T. M.; Lee, M. K.; Szabo, C. I.; Jerome, N.; McEuen, M.;
Taylor, M.; Hood, L.; King, M.-C.: Complete genomic sequence and
analysis of 117 kb of human DNA containing the gene BRCA1. Genome
Res. 6: 1029-1049, 1996.

*FIELD* CD
Paul J. Converse: 2/29/2000

*FIELD* ED
carol: 02/29/2000
carol: 2/29/2000

RBCC Red Blood Cell Collection

Full text data of VAT1