Full text data of VPS26B
VPS26B
[Confidence: low (only semi-automatic identification from reviews)]
Vacuolar protein sorting-associated protein 26B (Vesicle protein sorting 26B)
Vacuolar protein sorting-associated protein 26B (Vesicle protein sorting 26B)
UniProt
Q4G0F5
ID VP26B_HUMAN Reviewed; 336 AA.
AC Q4G0F5; Q96A55;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
read moreDT 25-JUL-2006, sequence version 2.
DT 22-JAN-2014, entry version 74.
DE RecName: Full=Vacuolar protein sorting-associated protein 26B;
DE AltName: Full=Vesicle protein sorting 26B;
GN Name=VPS26B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kim N.-S., Shon H.-Y., Oh J.-H., Lee J.-Y., Kim J.-M., Hahn Y.,
RA Park H.-S., Kim S., Kim Y.S.;
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304, AND MASS
RP SPECTROMETRY.
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, AND MASS
RP SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
CC -!- FUNCTION: Probable component of the retromer complex, a complex
CC required to retrieve lysosomal enzyme receptors (IGF2R and M6PR)
CC from endosomes to the trans-Golgi network (By similarity).
CC -!- SUBUNIT: Probable component of the retromer complex composed of
CC VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts
CC directly with VPS35 (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane
CC protein. Note=Does not localize to endosomes (By similarity).
CC -!- SIMILARITY: Belongs to the VPS26 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF452718; AAP13353.1; -; mRNA.
DR EMBL; BC009747; AAH09747.1; -; mRNA.
DR EMBL; BC014128; AAH14128.1; -; mRNA.
DR EMBL; BC098386; AAH98386.1; -; mRNA.
DR RefSeq; NP_443107.1; NM_052875.3.
DR UniGene; Hs.334684; -.
DR ProteinModelPortal; Q4G0F5; -.
DR SMR; Q4G0F5; 9-296.
DR IntAct; Q4G0F5; 1.
DR STRING; 9606.ENSP00000281187; -.
DR PhosphoSite; Q4G0F5; -.
DR DMDM; 110816482; -.
DR PaxDb; Q4G0F5; -.
DR PeptideAtlas; Q4G0F5; -.
DR PRIDE; Q4G0F5; -.
DR DNASU; 112936; -.
DR Ensembl; ENST00000281187; ENSP00000281187; ENSG00000151502.
DR Ensembl; ENST00000525095; ENSP00000434162; ENSG00000151502.
DR GeneID; 112936; -.
DR KEGG; hsa:112936; -.
DR UCSC; uc001qhe.3; human.
DR CTD; 112936; -.
DR GeneCards; GC11P134094; -.
DR HGNC; HGNC:28119; VPS26B.
DR HPA; HPA038172; -.
DR MIM; 610027; gene.
DR neXtProt; NX_Q4G0F5; -.
DR PharmGKB; PA128394747; -.
DR eggNOG; NOG256244; -.
DR HOGENOM; HOG000191799; -.
DR HOVERGEN; HBG082914; -.
DR InParanoid; Q4G0F5; -.
DR OMA; FDRGNHY; -.
DR OrthoDB; EOG7NW699; -.
DR PhylomeDB; Q4G0F5; -.
DR ChiTaRS; VPS26B; human.
DR GeneWiki; VPS26B; -.
DR GenomeRNAi; 112936; -.
DR NextBio; 78701; -.
DR PRO; PR:Q4G0F5; -.
DR Bgee; Q4G0F5; -.
DR CleanEx; HS_VPS26B; -.
DR Genevestigator; Q4G0F5; -.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0030904; C:retromer complex; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007034; P:vacuolar transport; IEA:InterPro.
DR InterPro; IPR005377; VPS26.
DR PANTHER; PTHR12233:SF1; PTHR12233:SF1; 1.
PE 1: Evidence at protein level;
KW Complete proteome; Cytoplasm; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1 336 Vacuolar protein sorting-associated
FT protein 26B.
FT /FTId=PRO_0000247089.
FT MOD_RES 302 302 Phosphoserine.
FT MOD_RES 304 304 Phosphoserine.
FT MOD_RES 319 319 Phosphoserine.
FT CONFLICT 55 55 K -> R (in Ref. 2; AAH98386).
SQ SEQUENCE 336 AA; 39155 MW; 1C07D0ABA7797A12 CRC64;
MSFFGFGQSV EVEILLNDAE SRKRAEHKTE DGKKEKYFLF YDGETVSGKV SLALKNPNKR
LEHQGIKIEF IGQIELYYDR GNHHEFVSLV KDLARPGEIT QSQAFDFEFT HVEKPYESYT
GQNVKLRYFL RATISRRLND VVKEMDIVVH TLSTYPELNS SIKMEVGIED CLHIEFEYNK
SKYHLKDVIV GKIYFLLVRI KIKHMEIDII KRETTGTGPN VYHENDTIAK YEIMDGAPVR
GESIPIRLFL AGYELTPTMR DINKKFSVRY YLNLVLIDEE ERRYFKQQEV VLWRKGDIVR
KSMSHQAAIA SQRFEGTTSL GEVRTPSQLS DNNCRQ
//
ID VP26B_HUMAN Reviewed; 336 AA.
AC Q4G0F5; Q96A55;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
read moreDT 25-JUL-2006, sequence version 2.
DT 22-JAN-2014, entry version 74.
DE RecName: Full=Vacuolar protein sorting-associated protein 26B;
DE AltName: Full=Vesicle protein sorting 26B;
GN Name=VPS26B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kim N.-S., Shon H.-Y., Oh J.-H., Lee J.-Y., Kim J.-M., Hahn Y.,
RA Park H.-S., Kim S., Kim Y.S.;
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304, AND MASS
RP SPECTROMETRY.
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, AND MASS
RP SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
CC -!- FUNCTION: Probable component of the retromer complex, a complex
CC required to retrieve lysosomal enzyme receptors (IGF2R and M6PR)
CC from endosomes to the trans-Golgi network (By similarity).
CC -!- SUBUNIT: Probable component of the retromer complex composed of
CC VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts
CC directly with VPS35 (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane
CC protein. Note=Does not localize to endosomes (By similarity).
CC -!- SIMILARITY: Belongs to the VPS26 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF452718; AAP13353.1; -; mRNA.
DR EMBL; BC009747; AAH09747.1; -; mRNA.
DR EMBL; BC014128; AAH14128.1; -; mRNA.
DR EMBL; BC098386; AAH98386.1; -; mRNA.
DR RefSeq; NP_443107.1; NM_052875.3.
DR UniGene; Hs.334684; -.
DR ProteinModelPortal; Q4G0F5; -.
DR SMR; Q4G0F5; 9-296.
DR IntAct; Q4G0F5; 1.
DR STRING; 9606.ENSP00000281187; -.
DR PhosphoSite; Q4G0F5; -.
DR DMDM; 110816482; -.
DR PaxDb; Q4G0F5; -.
DR PeptideAtlas; Q4G0F5; -.
DR PRIDE; Q4G0F5; -.
DR DNASU; 112936; -.
DR Ensembl; ENST00000281187; ENSP00000281187; ENSG00000151502.
DR Ensembl; ENST00000525095; ENSP00000434162; ENSG00000151502.
DR GeneID; 112936; -.
DR KEGG; hsa:112936; -.
DR UCSC; uc001qhe.3; human.
DR CTD; 112936; -.
DR GeneCards; GC11P134094; -.
DR HGNC; HGNC:28119; VPS26B.
DR HPA; HPA038172; -.
DR MIM; 610027; gene.
DR neXtProt; NX_Q4G0F5; -.
DR PharmGKB; PA128394747; -.
DR eggNOG; NOG256244; -.
DR HOGENOM; HOG000191799; -.
DR HOVERGEN; HBG082914; -.
DR InParanoid; Q4G0F5; -.
DR OMA; FDRGNHY; -.
DR OrthoDB; EOG7NW699; -.
DR PhylomeDB; Q4G0F5; -.
DR ChiTaRS; VPS26B; human.
DR GeneWiki; VPS26B; -.
DR GenomeRNAi; 112936; -.
DR NextBio; 78701; -.
DR PRO; PR:Q4G0F5; -.
DR Bgee; Q4G0F5; -.
DR CleanEx; HS_VPS26B; -.
DR Genevestigator; Q4G0F5; -.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0030904; C:retromer complex; IEA:InterPro.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007034; P:vacuolar transport; IEA:InterPro.
DR InterPro; IPR005377; VPS26.
DR PANTHER; PTHR12233:SF1; PTHR12233:SF1; 1.
PE 1: Evidence at protein level;
KW Complete proteome; Cytoplasm; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1 336 Vacuolar protein sorting-associated
FT protein 26B.
FT /FTId=PRO_0000247089.
FT MOD_RES 302 302 Phosphoserine.
FT MOD_RES 304 304 Phosphoserine.
FT MOD_RES 319 319 Phosphoserine.
FT CONFLICT 55 55 K -> R (in Ref. 2; AAH98386).
SQ SEQUENCE 336 AA; 39155 MW; 1C07D0ABA7797A12 CRC64;
MSFFGFGQSV EVEILLNDAE SRKRAEHKTE DGKKEKYFLF YDGETVSGKV SLALKNPNKR
LEHQGIKIEF IGQIELYYDR GNHHEFVSLV KDLARPGEIT QSQAFDFEFT HVEKPYESYT
GQNVKLRYFL RATISRRLND VVKEMDIVVH TLSTYPELNS SIKMEVGIED CLHIEFEYNK
SKYHLKDVIV GKIYFLLVRI KIKHMEIDII KRETTGTGPN VYHENDTIAK YEIMDGAPVR
GESIPIRLFL AGYELTPTMR DINKKFSVRY YLNLVLIDEE ERRYFKQQEV VLWRKGDIVR
KSMSHQAAIA SQRFEGTTSL GEVRTPSQLS DNNCRQ
//
MIM
610027
*RECORD*
*FIELD* NO
610027
*FIELD* TI
*610027 VACUOLAR PROTEIN SORTING 26, YEAST, HOMOLOG OF, B; VPS26B
*FIELD* TX
CLONING
read more
Kerr et al. (2005) identified mouse Vps26b, which encodes a deduced
336-amino acid protein with a calculated molecular mass of 39.1 kD.
Microarray data analysis showed that Vps26b was expressed in all mouse
tissues examined. In situ hybridization of mouse embryos detected
tissue- and stage-specific expression of Vps26b at all developmental
stages examined. Transfected HeLa cells expressed mouse Vps26b in the
cytoplasm, with low levels at the plasma membrane. Within a human lung
carcinoma cell line, endogenous VPS26B and VPS26A (605506) colocalized
with VPS35 (606931) in actin-rich lamellipodia at the cell surface.
GENE FUNCTION
By coprecipitation analysis using transfected human cell lines and yeast
2-hybrid analysis, Kerr et al. (2005) confirmed that mouse Vps26b
interacts directly with Vps35.
GENE STRUCTURE
Kerr et al. (2005) determined that the human and mouse VPS26B genes
contain 6 exons.
MAPPING
By genomic sequence analysis, Kerr et al. (2005) mapped the VPS26B gene
to chromosome 11q25. They mapped the mouse Vps26b gene to chromosome
9A4.
*FIELD* RF
1. Kerr, M. C.; Bennetts, J. S.; Simpson, F.; Thomas, E. C.; Flegg,
C.; Gleeson, P. A.; Wicking, C.; Teasdale, R. D.: A novel mammalian
retromer component, Vps26B. Traffic 6: 991-1001, 2005.
*FIELD* CD
Patricia A. Hartz: 4/7/2006
*FIELD* ED
mgross: 04/07/2006
*RECORD*
*FIELD* NO
610027
*FIELD* TI
*610027 VACUOLAR PROTEIN SORTING 26, YEAST, HOMOLOG OF, B; VPS26B
*FIELD* TX
CLONING
read more
Kerr et al. (2005) identified mouse Vps26b, which encodes a deduced
336-amino acid protein with a calculated molecular mass of 39.1 kD.
Microarray data analysis showed that Vps26b was expressed in all mouse
tissues examined. In situ hybridization of mouse embryos detected
tissue- and stage-specific expression of Vps26b at all developmental
stages examined. Transfected HeLa cells expressed mouse Vps26b in the
cytoplasm, with low levels at the plasma membrane. Within a human lung
carcinoma cell line, endogenous VPS26B and VPS26A (605506) colocalized
with VPS35 (606931) in actin-rich lamellipodia at the cell surface.
GENE FUNCTION
By coprecipitation analysis using transfected human cell lines and yeast
2-hybrid analysis, Kerr et al. (2005) confirmed that mouse Vps26b
interacts directly with Vps35.
GENE STRUCTURE
Kerr et al. (2005) determined that the human and mouse VPS26B genes
contain 6 exons.
MAPPING
By genomic sequence analysis, Kerr et al. (2005) mapped the VPS26B gene
to chromosome 11q25. They mapped the mouse Vps26b gene to chromosome
9A4.
*FIELD* RF
1. Kerr, M. C.; Bennetts, J. S.; Simpson, F.; Thomas, E. C.; Flegg,
C.; Gleeson, P. A.; Wicking, C.; Teasdale, R. D.: A novel mammalian
retromer component, Vps26B. Traffic 6: 991-1001, 2005.
*FIELD* CD
Patricia A. Hartz: 4/7/2006
*FIELD* ED
mgross: 04/07/2006