DX39B_HUMAN

Source: PM19886704
Marked as 'Non-membrane protein'
Confidence: low (only semi-automatic identification from reviews) Search PubMed for
(RBC AND this entry)
Gene names: DDX39B , BAT1, UAP56
Protein names and data: DX39B_HUMAN , Spliceosome RNA helicase DDX39B; 3.6.4.13 , 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein Lenght: 428 a.a.
Mass: 48991 Da
fasta formatted sequence
Function: Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription- independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability. Splice factor that is required for the first ATP- dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect.
Catalytic activity: ATP + H(2)O = ADP + phosphate.
Cellular location: Nucleus. Nucleus speckle. Cytoplasm. Note=Can translocate to the cytoplasm in the presence of MX1. TREX complex assembly seems to occur in regions surrounding nuclear speckles known as perispeckles.

Database cross-references

UniProt: Q13838
Ensembl: ENST00000383508
Ensembl: ENST00000396172
Ensembl: ENST00000400295
Ensembl: ENST00000400296
Ensembl: ENST00000412106
Ensembl: ENST00000412330
Ensembl: ENST00000413678
Ensembl: ENST00000414440
Ensembl: ENST00000415689
Ensembl: ENST00000416863
Ensembl: ENST00000430784
Ensembl: ENST00000431360
Ensembl: ENST00000441425
Ensembl: ENST00000445218
Ensembl: ENST00000448296
Ensembl: ENST00000453138
Ensembl: ENST00000456476
Ensembl: ENST00000456666
Ensembl: ENST00000458640
MIM: 142560
neXtProt: NX_Q13838
Antibodypedia: Q13838 (may not find the protein thus also not any antibody)
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