Full text data of CD8B
CD8B
(CD8B1)
[Confidence: low (only semi-automatic identification from reviews)]
T-cell surface glycoprotein CD8 beta chain (CD8b; Flags: Precursor)
T-cell surface glycoprotein CD8 beta chain (CD8b; Flags: Precursor)
UniProt
P10966
ID CD8B_HUMAN Reviewed; 210 AA.
AC P10966; P14860; P14861; Q15980; Q496E0; Q496E1; Q496E2; Q9UDB4;
read moreAC Q9UDB5; Q9UDB6; Q9UDB7; Q9UDB8; Q9UDB9; Q9UDC0; Q9UQ55;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 22-JAN-2014, entry version 150.
DE RecName: Full=T-cell surface glycoprotein CD8 beta chain;
DE AltName: CD_antigen=CD8b;
DE Flags: Precursor;
GN Name=CD8B; Synonyms=CD8B1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=T-cell;
RX PubMed=3145195;
RA Norment A.M., Littman D.R.;
RT "A second subunit of CD8 is expressed in human T cells.";
RL EMBO J. 7:3433-3439(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=3145196;
RA Disanto J.P., Knowles R.W., Flomenberg N.;
RT "The human Lyt-3 molecule requires CD8 for cell surface expression.";
RL EMBO J. 7:3465-3470(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=3264320; DOI=10.1084/jem.168.6.1993;
RA Shiue L., Gorman S.D., Parnes J.R.;
RT "A second chain of human CD8 is expressed on peripheral blood
RT lymphocytes.";
RL J. Exp. Med. 168:1993-2005(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2; 3 AND 4).
RX PubMed=1541829;
RA Nakayama K., Kawachi Y., Tokito S., Minami N., Yamamoto R., Imai T.,
RA Gachelin G., Nakauchi H.;
RT "Recent duplication of the two human CD8 beta-chain genes.";
RL J. Immunol. 148:1919-1927(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND 8).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 162-210 (ISOFORMS 1; 2; 3; 5; 6; 7 AND
RP 8), ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RC TISSUE=Peripheral blood;
RX PubMed=8436166; DOI=10.1002/eji.1830230203;
RA DiSanto J.P., Smith D., de Bruin D., Lacy E., Flomenberg N.;
RT "Transcriptional diversity at the duplicated human CD8 beta loci.";
RL Eur. J. Immunol. 23:320-326(1993).
CC -!- FUNCTION: Identifies cytotoxic/suppressor T-cells that interact
CC with MHC class I bearing targets. CD8 is thought to play a role in
CC the process of T-cell mediated killing.
CC -!- SUBUNIT: In general heterodimer of an alpha and a beta chain
CC linked by two disulfide bonds.
CC -!- SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 2: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 3: Secreted (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 4: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 5: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 6: Secreted (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 7: Secreted (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 8: Secreted (Probable).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=8;
CC Name=1; Synonyms=M-1, Mbeta1;
CC IsoId=P10966-1; Sequence=Displayed;
CC Name=2; Synonyms=M-3, Mbeta2;
CC IsoId=P10966-2; Sequence=VSP_002490;
CC Name=3; Synonyms=S-1, Sbeta3;
CC IsoId=P10966-3; Sequence=VSP_002492, VSP_002493;
CC Name=4; Synonyms=M-2;
CC IsoId=P10966-4; Sequence=VSP_002491;
CC Name=5; Synonyms=Mbeta3;
CC IsoId=P10966-6; Sequence=VSP_002493;
CC Note=Ref.6 (AAI00915) sequence is in conflict in position:
CC 213:I->V;
CC Name=6; Synonyms=Sbeta1;
CC IsoId=P10966-7; Sequence=VSP_002492;
CC Name=7; Synonyms=Sbeta4;
CC IsoId=P10966-8; Sequence=VSP_039654;
CC Name=8; Synonyms=Sbeta5;
CC IsoId=P10966-9; Sequence=VSP_039655;
CC -!- TISSUE SPECIFICITY: Isoform 1, isoform 3, isoform 5, isoform 6,
CC isoform 7 and isoform 8 are expressed in both thymus and
CC peripheral CD8+ T-cells. Expression of isoform 1 is higher in
CC thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of
CC isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+
CC T-cells.
CC -!- PTM: Phosphorylated as a consequence of T-cell activation
CC (Potential).
CC -!- SIMILARITY: Contains 1 Ig-like V-type (immunoglobulin-like)
CC domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD13877.1; Type=Erroneous gene model prediction;
CC -!- WEB RESOURCE: Name=Wikipedia; Note=CD8 entry;
CC URL="http://en.wikipedia.org/wiki/CD8";
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; X13444; CAA31795.1; -; mRNA.
DR EMBL; X13445; CAA31796.1; -; mRNA.
DR EMBL; X13446; CAA31797.1; -; mRNA.
DR EMBL; X13452; CAA31803.1; -; mRNA.
DR EMBL; Y00805; CAA68750.1; -; mRNA.
DR EMBL; M36712; AAA35664.1; -; mRNA.
DR EMBL; S87090; AAB21669.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87087; AAB21670.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87078; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87083; AAB21671.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87078; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87083; AAB21672.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87078; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; AC111200; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC100911; AAI00912.1; -; mRNA.
DR EMBL; BC100912; AAI00913.1; -; mRNA.
DR EMBL; BC100913; AAI00914.1; -; mRNA.
DR EMBL; BC100914; AAI00915.1; -; mRNA.
DR EMBL; S55731; AAD13877.1; ALT_SEQ; Genomic_DNA.
DR EMBL; S55730; AAD13877.1; JOINED; Genomic_DNA.
DR EMBL; S55733; AAD13877.1; JOINED; Genomic_DNA.
DR PIR; C49050; C49050.
DR PIR; D46482; D46482.
DR PIR; E49050; E49050.
DR PIR; G49050; G49050.
DR PIR; S01647; E46482.
DR PIR; S01873; C46482.
DR PIR; S01874; B46482.
DR PIR; T01073; T01073.
DR RefSeq; NP_001171571.1; NM_001178100.1.
DR RefSeq; NP_004922.1; NM_004931.4.
DR RefSeq; NP_742099.1; NM_172101.3.
DR RefSeq; NP_742100.1; NM_172102.3.
DR RefSeq; NP_757362.1; NM_172213.3.
DR UniGene; Hs.405667; -.
DR ProteinModelPortal; P10966; -.
DR SMR; P10966; 22-163.
DR STRING; 9606.ENSP00000331172; -.
DR PhosphoSite; P10966; -.
DR DMDM; 116032; -.
DR PaxDb; P10966; -.
DR PRIDE; P10966; -.
DR DNASU; 926; -.
DR Ensembl; ENST00000331469; ENSP00000331172; ENSG00000172116.
DR Ensembl; ENST00000349455; ENSP00000340592; ENSG00000172116.
DR Ensembl; ENST00000390655; ENSP00000375070; ENSG00000172116.
DR Ensembl; ENST00000393759; ENSP00000377356; ENSG00000172116.
DR Ensembl; ENST00000393761; ENSP00000377358; ENSG00000172116.
DR GeneID; 926; -.
DR KEGG; hsa:926; -.
DR UCSC; uc002srz.3; human.
DR CTD; 926; -.
DR GeneCards; GC02M087042; -.
DR HGNC; HGNC:1707; CD8B.
DR HPA; CAB004353; -.
DR MIM; 186730; gene.
DR neXtProt; NX_P10966; -.
DR PharmGKB; PA26245; -.
DR eggNOG; NOG45880; -.
DR HOVERGEN; HBG105700; -.
DR KO; K06459; -.
DR OMA; IYWLRQR; -.
DR OrthoDB; EOG7NSB5C; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_6900; Immune System.
DR GenomeRNAi; 926; -.
DR NextBio; 3832; -.
DR PRO; PR:P10966; -.
DR ArrayExpress; P10966; -.
DR Bgee; P10966; -.
DR CleanEx; HS_CD8B; -.
DR Genevestigator; P10966; -.
DR GO; GO:0031901; C:early endosome membrane; TAS:Reactome.
DR GO; GO:0009897; C:external side of plasma membrane; IEA:Ensembl.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR GO; GO:0042101; C:T cell receptor complex; NAS:UniProtKB.
DR GO; GO:0015026; F:coreceptor activity; NAS:UniProtKB.
DR GO; GO:0042288; F:MHC class I protein binding; NAS:UniProtKB.
DR GO; GO:0006955; P:immune response; NAS:UniProtKB.
DR GO; GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome.
DR GO; GO:0050776; P:regulation of immune response; TAS:Reactome.
DR GO; GO:0042110; P:T cell activation; NAS:UniProtKB.
DR GO; GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; NAS:UniProtKB.
DR GO; GO:0016032; P:viral process; TAS:Reactome.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Adaptive immunity; Alternative splicing; Cell membrane;
KW Complete proteome; Disulfide bond; Glycoprotein; Immunity;
KW Immunoglobulin domain; Membrane; Phosphoprotein; Reference proteome;
KW Secreted; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1 21
FT CHAIN 22 210 T-cell surface glycoprotein CD8 beta
FT chain.
FT /FTId=PRO_0000014643.
FT TOPO_DOM 22 170 Extracellular (Potential).
FT TRANSMEM 171 191 Helical; (Potential).
FT TOPO_DOM 192 210 Cytoplasmic (Potential).
FT DOMAIN 22 132 Ig-like V-type.
FT MOD_RES 209 209 Phosphotyrosine (Potential).
FT CARBOHYD 102 102 N-linked (GlcNAc...) (Potential).
FT DISULFID 41 116 Potential.
FT VAR_SEQ 165 210 GPLCSPITLGLLVAGVLVLLVSLGVAIHLCCRRRRARLRFM
FT KQFYK -> DFTNKQRIGFWCPATKRHRSVMSTMWKNERRD
FT TFNPGEFNGC (in isoform 7).
FT /FTId=VSP_039654.
FT VAR_SEQ 166 210 PLCSPITLGLLVAGVLVLLVSLGVAIHLCCRRRRARLRFMK
FT QFYK -> LKGKVYQEPLSPNACMDTTAILQPHRSCLTHGS
FT (in isoform 8).
FT /FTId=VSP_039655.
FT VAR_SEQ 166 195 Missing (in isoform 3 and isoform 6).
FT /FTId=VSP_002492.
FT VAR_SEQ 208 210 FYK -> LRLHPLEKCSRMDY (in isoform 2).
FT /FTId=VSP_002490.
FT VAR_SEQ 208 210 FYK -> PQGEGISGTFVPQCLHGYYSNTTTSQKLLNPWIL
FT KT (in isoform 3 and isoform 5).
FT /FTId=VSP_002493.
FT VAR_SEQ 208 210 FYK -> KFNIVCLKISGFTTCCCFQILQISREYGFGVLLQ
FT KDIGQ (in isoform 4).
FT /FTId=VSP_002491.
FT CONFLICT 173 173 Missing (in Ref. 7; no nucleotide entry).
FT CONFLICT 180 180 V -> I (in Ref. 6; AAI00915).
SQ SEQUENCE 210 AA; 23722 MW; 675AD919585F4B80 CRC64;
MRPRLWLLLA AQLTVLHGNS VLQQTPAYIK VQTNKMVMLS CEAKISLSNM RIYWLRQRQA
PSSDSHHEFL ALWDSAKGTI HGEEVEQEKI AVFRDASRFI LNLTSVKPED SGIYFCMIVG
SPELTFGKGT QLSVVDFLPT TAQPTKKSTL KKRVCRLPRP ETQKGPLCSP ITLGLLVAGV
LVLLVSLGVA IHLCCRRRRA RLRFMKQFYK
//
ID CD8B_HUMAN Reviewed; 210 AA.
AC P10966; P14860; P14861; Q15980; Q496E0; Q496E1; Q496E2; Q9UDB4;
read moreAC Q9UDB5; Q9UDB6; Q9UDB7; Q9UDB8; Q9UDB9; Q9UDC0; Q9UQ55;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 22-JAN-2014, entry version 150.
DE RecName: Full=T-cell surface glycoprotein CD8 beta chain;
DE AltName: CD_antigen=CD8b;
DE Flags: Precursor;
GN Name=CD8B; Synonyms=CD8B1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=T-cell;
RX PubMed=3145195;
RA Norment A.M., Littman D.R.;
RT "A second subunit of CD8 is expressed in human T cells.";
RL EMBO J. 7:3433-3439(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=3145196;
RA Disanto J.P., Knowles R.W., Flomenberg N.;
RT "The human Lyt-3 molecule requires CD8 for cell surface expression.";
RL EMBO J. 7:3465-3470(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=3264320; DOI=10.1084/jem.168.6.1993;
RA Shiue L., Gorman S.D., Parnes J.R.;
RT "A second chain of human CD8 is expressed on peripheral blood
RT lymphocytes.";
RL J. Exp. Med. 168:1993-2005(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2; 3 AND 4).
RX PubMed=1541829;
RA Nakayama K., Kawachi Y., Tokito S., Minami N., Yamamoto R., Imai T.,
RA Gachelin G., Nakauchi H.;
RT "Recent duplication of the two human CD8 beta-chain genes.";
RL J. Immunol. 148:1919-1927(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 5 AND 8).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 162-210 (ISOFORMS 1; 2; 3; 5; 6; 7 AND
RP 8), ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RC TISSUE=Peripheral blood;
RX PubMed=8436166; DOI=10.1002/eji.1830230203;
RA DiSanto J.P., Smith D., de Bruin D., Lacy E., Flomenberg N.;
RT "Transcriptional diversity at the duplicated human CD8 beta loci.";
RL Eur. J. Immunol. 23:320-326(1993).
CC -!- FUNCTION: Identifies cytotoxic/suppressor T-cells that interact
CC with MHC class I bearing targets. CD8 is thought to play a role in
CC the process of T-cell mediated killing.
CC -!- SUBUNIT: In general heterodimer of an alpha and a beta chain
CC linked by two disulfide bonds.
CC -!- SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 2: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 3: Secreted (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 4: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 5: Cell membrane; Single-pass type I
CC membrane protein (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 6: Secreted (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 7: Secreted (Probable).
CC -!- SUBCELLULAR LOCATION: Isoform 8: Secreted (Probable).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=8;
CC Name=1; Synonyms=M-1, Mbeta1;
CC IsoId=P10966-1; Sequence=Displayed;
CC Name=2; Synonyms=M-3, Mbeta2;
CC IsoId=P10966-2; Sequence=VSP_002490;
CC Name=3; Synonyms=S-1, Sbeta3;
CC IsoId=P10966-3; Sequence=VSP_002492, VSP_002493;
CC Name=4; Synonyms=M-2;
CC IsoId=P10966-4; Sequence=VSP_002491;
CC Name=5; Synonyms=Mbeta3;
CC IsoId=P10966-6; Sequence=VSP_002493;
CC Note=Ref.6 (AAI00915) sequence is in conflict in position:
CC 213:I->V;
CC Name=6; Synonyms=Sbeta1;
CC IsoId=P10966-7; Sequence=VSP_002492;
CC Name=7; Synonyms=Sbeta4;
CC IsoId=P10966-8; Sequence=VSP_039654;
CC Name=8; Synonyms=Sbeta5;
CC IsoId=P10966-9; Sequence=VSP_039655;
CC -!- TISSUE SPECIFICITY: Isoform 1, isoform 3, isoform 5, isoform 6,
CC isoform 7 and isoform 8 are expressed in both thymus and
CC peripheral CD8+ T-cells. Expression of isoform 1 is higher in
CC thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of
CC isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+
CC T-cells.
CC -!- PTM: Phosphorylated as a consequence of T-cell activation
CC (Potential).
CC -!- SIMILARITY: Contains 1 Ig-like V-type (immunoglobulin-like)
CC domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD13877.1; Type=Erroneous gene model prediction;
CC -!- WEB RESOURCE: Name=Wikipedia; Note=CD8 entry;
CC URL="http://en.wikipedia.org/wiki/CD8";
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; X13444; CAA31795.1; -; mRNA.
DR EMBL; X13445; CAA31796.1; -; mRNA.
DR EMBL; X13446; CAA31797.1; -; mRNA.
DR EMBL; X13452; CAA31803.1; -; mRNA.
DR EMBL; Y00805; CAA68750.1; -; mRNA.
DR EMBL; M36712; AAA35664.1; -; mRNA.
DR EMBL; S87090; AAB21669.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21669.2; JOINED; Genomic_DNA.
DR EMBL; S87087; AAB21670.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87078; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21670.2; JOINED; Genomic_DNA.
DR EMBL; S87083; AAB21671.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87078; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21671.2; JOINED; Genomic_DNA.
DR EMBL; S87083; AAB21672.2; -; Genomic_DNA.
DR EMBL; S87068; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87070; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87073; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87078; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; S87081; AAB21672.2; JOINED; Genomic_DNA.
DR EMBL; AC111200; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC100911; AAI00912.1; -; mRNA.
DR EMBL; BC100912; AAI00913.1; -; mRNA.
DR EMBL; BC100913; AAI00914.1; -; mRNA.
DR EMBL; BC100914; AAI00915.1; -; mRNA.
DR EMBL; S55731; AAD13877.1; ALT_SEQ; Genomic_DNA.
DR EMBL; S55730; AAD13877.1; JOINED; Genomic_DNA.
DR EMBL; S55733; AAD13877.1; JOINED; Genomic_DNA.
DR PIR; C49050; C49050.
DR PIR; D46482; D46482.
DR PIR; E49050; E49050.
DR PIR; G49050; G49050.
DR PIR; S01647; E46482.
DR PIR; S01873; C46482.
DR PIR; S01874; B46482.
DR PIR; T01073; T01073.
DR RefSeq; NP_001171571.1; NM_001178100.1.
DR RefSeq; NP_004922.1; NM_004931.4.
DR RefSeq; NP_742099.1; NM_172101.3.
DR RefSeq; NP_742100.1; NM_172102.3.
DR RefSeq; NP_757362.1; NM_172213.3.
DR UniGene; Hs.405667; -.
DR ProteinModelPortal; P10966; -.
DR SMR; P10966; 22-163.
DR STRING; 9606.ENSP00000331172; -.
DR PhosphoSite; P10966; -.
DR DMDM; 116032; -.
DR PaxDb; P10966; -.
DR PRIDE; P10966; -.
DR DNASU; 926; -.
DR Ensembl; ENST00000331469; ENSP00000331172; ENSG00000172116.
DR Ensembl; ENST00000349455; ENSP00000340592; ENSG00000172116.
DR Ensembl; ENST00000390655; ENSP00000375070; ENSG00000172116.
DR Ensembl; ENST00000393759; ENSP00000377356; ENSG00000172116.
DR Ensembl; ENST00000393761; ENSP00000377358; ENSG00000172116.
DR GeneID; 926; -.
DR KEGG; hsa:926; -.
DR UCSC; uc002srz.3; human.
DR CTD; 926; -.
DR GeneCards; GC02M087042; -.
DR HGNC; HGNC:1707; CD8B.
DR HPA; CAB004353; -.
DR MIM; 186730; gene.
DR neXtProt; NX_P10966; -.
DR PharmGKB; PA26245; -.
DR eggNOG; NOG45880; -.
DR HOVERGEN; HBG105700; -.
DR KO; K06459; -.
DR OMA; IYWLRQR; -.
DR OrthoDB; EOG7NSB5C; -.
DR Reactome; REACT_116125; Disease.
DR Reactome; REACT_6900; Immune System.
DR GenomeRNAi; 926; -.
DR NextBio; 3832; -.
DR PRO; PR:P10966; -.
DR ArrayExpress; P10966; -.
DR Bgee; P10966; -.
DR CleanEx; HS_CD8B; -.
DR Genevestigator; P10966; -.
DR GO; GO:0031901; C:early endosome membrane; TAS:Reactome.
DR GO; GO:0009897; C:external side of plasma membrane; IEA:Ensembl.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR GO; GO:0042101; C:T cell receptor complex; NAS:UniProtKB.
DR GO; GO:0015026; F:coreceptor activity; NAS:UniProtKB.
DR GO; GO:0042288; F:MHC class I protein binding; NAS:UniProtKB.
DR GO; GO:0006955; P:immune response; NAS:UniProtKB.
DR GO; GO:0050690; P:regulation of defense response to virus by virus; TAS:Reactome.
DR GO; GO:0050776; P:regulation of immune response; TAS:Reactome.
DR GO; GO:0042110; P:T cell activation; NAS:UniProtKB.
DR GO; GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; NAS:UniProtKB.
DR GO; GO:0016032; P:viral process; TAS:Reactome.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR Pfam; PF07686; V-set; 1.
DR SMART; SM00409; IG; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Adaptive immunity; Alternative splicing; Cell membrane;
KW Complete proteome; Disulfide bond; Glycoprotein; Immunity;
KW Immunoglobulin domain; Membrane; Phosphoprotein; Reference proteome;
KW Secreted; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1 21
FT CHAIN 22 210 T-cell surface glycoprotein CD8 beta
FT chain.
FT /FTId=PRO_0000014643.
FT TOPO_DOM 22 170 Extracellular (Potential).
FT TRANSMEM 171 191 Helical; (Potential).
FT TOPO_DOM 192 210 Cytoplasmic (Potential).
FT DOMAIN 22 132 Ig-like V-type.
FT MOD_RES 209 209 Phosphotyrosine (Potential).
FT CARBOHYD 102 102 N-linked (GlcNAc...) (Potential).
FT DISULFID 41 116 Potential.
FT VAR_SEQ 165 210 GPLCSPITLGLLVAGVLVLLVSLGVAIHLCCRRRRARLRFM
FT KQFYK -> DFTNKQRIGFWCPATKRHRSVMSTMWKNERRD
FT TFNPGEFNGC (in isoform 7).
FT /FTId=VSP_039654.
FT VAR_SEQ 166 210 PLCSPITLGLLVAGVLVLLVSLGVAIHLCCRRRRARLRFMK
FT QFYK -> LKGKVYQEPLSPNACMDTTAILQPHRSCLTHGS
FT (in isoform 8).
FT /FTId=VSP_039655.
FT VAR_SEQ 166 195 Missing (in isoform 3 and isoform 6).
FT /FTId=VSP_002492.
FT VAR_SEQ 208 210 FYK -> LRLHPLEKCSRMDY (in isoform 2).
FT /FTId=VSP_002490.
FT VAR_SEQ 208 210 FYK -> PQGEGISGTFVPQCLHGYYSNTTTSQKLLNPWIL
FT KT (in isoform 3 and isoform 5).
FT /FTId=VSP_002493.
FT VAR_SEQ 208 210 FYK -> KFNIVCLKISGFTTCCCFQILQISREYGFGVLLQ
FT KDIGQ (in isoform 4).
FT /FTId=VSP_002491.
FT CONFLICT 173 173 Missing (in Ref. 7; no nucleotide entry).
FT CONFLICT 180 180 V -> I (in Ref. 6; AAI00915).
SQ SEQUENCE 210 AA; 23722 MW; 675AD919585F4B80 CRC64;
MRPRLWLLLA AQLTVLHGNS VLQQTPAYIK VQTNKMVMLS CEAKISLSNM RIYWLRQRQA
PSSDSHHEFL ALWDSAKGTI HGEEVEQEKI AVFRDASRFI LNLTSVKPED SGIYFCMIVG
SPELTFGKGT QLSVVDFLPT TAQPTKKSTL KKRVCRLPRP ETQKGPLCSP ITLGLLVAGV
LVLLVSLGVA IHLCCRRRRA RLRFMKQFYK
//
MIM
186730
*RECORD*
*FIELD* NO
186730
*FIELD* TI
*186730 CD8 ANTIGEN, BETA POLYPEPTIDE; CD8B
;;T-CELL GLYCOPROTEIN CD8B;;
CD8B1
*FIELD* TX
read more
DESCRIPTION
The T-cell differentiation antigen CD8 is a glycoprotein present at the
surface of thymocytes and T lymphocytes that recognizes antigenic
peptides in the context of the class I major histocompatibility complex
(MHC) molecules. In the human, the CD8 antigen is a heterodimeric
product of the association of an alpha (CD8A; 186910) and a beta chain
(CD8B) (Delarbre et al., 1993).
CLONING
CD8 is a T-cell glycoprotein expressed only in cytotoxic T cells that
recognize antigen in context with class I major histocompatibility
antigens. Antibodies against CD8 can block the killing activity of these
cells, the inhibition occurring at the level of target cell adhesion.
Mouse CD8 consists of 2 distinct chains, Ly-2 (Cd8a) and Ly-3 (Cd8b1);
rat CD8, called Ox-8, also has 2 chains. Human CD8 was thought to be
made up of only 1 chain in mature T cells. However, Johnson (1987)
isolated CD8B, a human gene homologous to the gene encoding the second
rodent CD8 chain, and showed that it is transcribed in human thymocytes
and in some acute T-cell leukemias.
MAPPING
By analysis of a panel of 14 human-rodent somatic cell hybrids, Spurr et
al. (1988) found that CD8B maps to human chromosome 2. In the mouse, the
genes that encode the 2 CD8 chains are closely linked on chromosome 6.
No recombination was observed in 370 meiotic events. These genes are
also closely linked to the immunoglobulin kappa light chain cluster in
the mouse. The linkage between CD8A (186910) and the kappa locus is also
conserved in man. Close linkage is of interest since these proteins all
belong to the immunoglobulin superfamily.
Nakayama et al. (1992) discovered a second but inactive CD8 beta-chain
gene, which they named CD8B2, the functional gene being renamed CD8B1.
The nucleotide sequences of the coding and noncoding regions of CD8B1
and CD8B2 were found to be 98.9% homologous, suggesting that the
duplication event had occurred rather recently in the evolution of the
human lineage. They showed that the CD8B pseudogene was present in all
human Japanese and Caucasian samples tested. Delarbre et al. (1993)
showed that the pseudogene is missing exons 8 and 9 of CD8B. From a
study of somatic cell hybrids, they concluded that, like CD8A and CD8B,
the pseudogene is located on chromosome 2. Pulsed-field gel
electrophoresis experiments demonstrated that the pseudogene is at least
1,500 kb removed from the functional gene, CD8B.
Zhang et al. (1996) demonstrated, by fluorescence in situ hybridization,
that the second CD8B gene locus in the human maps to 2q12, a position on
the opposite side of the centromere from the first locus.
EVOLUTION
Delarbre et al. (1993) demonstrated that the duplication of the CD8B
gene had taken place in an ancestor common to the human, gorilla, and
chimpanzee clade, most likely excluding the orangutan and other
primates.
GENE FUNCTION
To locate factors regulating the expression of CD8 and the functional
commitment of CD8-positive T cells, Kieffer et al. (2002) identified
SATB1 (602075)- and GATA3 (131320)-binding sites in matrix attachment
regions and DNase I hypersensitive sites at the 3-prime end of the CD8B
gene. They proposed that this region is an epigenetic regulator of CD8
expression.
In mice, at least 5 Cd8 cis-acting enhancers, individually or in
combination, direct expression in the T-cell lineage, and deletion of
specific enhancers leads to variegated expression of Cd8a/Cd8b
heterodimers in double-positive thymocytes. Bilic et al. (2006) showed
that Cd8 variegation due to enhancer deletion correlated with an
epigenetic 'off' state, linking Cd8 enhancer function with chromatin
remodeling of Cd8a and Cd8b. The zinc finger protein Mazr (ZNF278;
605165) bound the Cd8 enhancer and, via its N-terminal domain,
interacted with the Ncor1 (600849) complex in double-negative
thymocytes. Mazr was downregulated in double-positive and Cd8
single-positive thymocytes. Constitutive expression of Mazr during
T-cell development resulted in variegated expression of Cd8 in
double-positive thymocytes. Bilic et al. (2006) concluded that MAZR is a
negative regulator of CD8 expression in double-negative thymocytes.
*FIELD* RF
1. Bilic, I.; Koesters, C.; Unger, B.; Sekimata, M.; Hertweck, A.;
Maschek, R.; Wilson, C. B.; Ellmeier, W.: Negative regulation of
CD8 expression via Cd8 enhancer-mediated recruitment of the zinc finger
protein MAZR. Nature Immun. 7: 392-400, 2006.
2. Delarbre, C.; Nakauchi, H.; Bontrop, R.; Kourilsky, P.; Gachelin,
G.: Duplication of the CD8 beta-chain gene as a marker of the man-gorilla-chimpanzee
clade. Proc. Nat. Acad. Sci. 90: 7049-7053, 1993.
3. Johnson, P.: A human homolog of the mouse CD8 molecule, Lyt-3:
genomic sequence and expression. Immunogenetics 26: 174-177, 1987.
4. Kieffer, L. J.; Greally, J. M.; Landres, I.; Nag, S.; Nakajima,
Y.; Kohwi-Shigematsu, T.; Kavathas, P. B.: Identification of a candidate
regulatory region in the human CD8 gene complex by colocalization
of DNase I hypersensitive sites and matrix attachment regions which
bind SATB1 and GATA-3. J. Immun. 168: 3915-3922, 2002.
5. Nakayama, K. I.; Kawachi, Y.; Tokito, S.; Minami, N.; Yamamoto,
R.; Imai, T.; Gachelin, G.; Nakauchi, H.: Recent duplication of the
two human CD8 beta-chain genes. J. Immun. 148: 1919-1927, 1992.
6. Spurr, N. K.; Goodfellow, P. N.; Johnson, P.: CD8B, the human
equivalent of the mouse Ly-3 gene is localized on chromosome 2. Immunogenetics 27:
70-72, 1988.
7. Zhang, X.-L.; Heng, H. H. Q.; Yang, Y.; Tsui, L.-C.; Parnes, J.
R.; Chamberlain, J. W.: Chromosomal mapping of the second human CD8B
gene locus. Immunogenetics 43: 220-226, 1996.
*FIELD* CN
Paul J. Converse - updated: 2/16/2007
Paul J. Converse - updated: 5/16/2002
*FIELD* CD
Victor A. McKusick: 2/12/1988
*FIELD* ED
carol: 07/30/2010
carol: 7/30/2010
mgross: 2/16/2007
mgross: 5/16/2002
dkim: 7/23/1998
mark: 3/28/1996
terry: 3/20/1996
carol: 10/12/1993
carol: 10/4/1993
supermim: 3/16/1992
supermim: 3/20/1990
ddp: 10/27/1989
carol: 6/19/1989
*RECORD*
*FIELD* NO
186730
*FIELD* TI
*186730 CD8 ANTIGEN, BETA POLYPEPTIDE; CD8B
;;T-CELL GLYCOPROTEIN CD8B;;
CD8B1
*FIELD* TX
read more
DESCRIPTION
The T-cell differentiation antigen CD8 is a glycoprotein present at the
surface of thymocytes and T lymphocytes that recognizes antigenic
peptides in the context of the class I major histocompatibility complex
(MHC) molecules. In the human, the CD8 antigen is a heterodimeric
product of the association of an alpha (CD8A; 186910) and a beta chain
(CD8B) (Delarbre et al., 1993).
CLONING
CD8 is a T-cell glycoprotein expressed only in cytotoxic T cells that
recognize antigen in context with class I major histocompatibility
antigens. Antibodies against CD8 can block the killing activity of these
cells, the inhibition occurring at the level of target cell adhesion.
Mouse CD8 consists of 2 distinct chains, Ly-2 (Cd8a) and Ly-3 (Cd8b1);
rat CD8, called Ox-8, also has 2 chains. Human CD8 was thought to be
made up of only 1 chain in mature T cells. However, Johnson (1987)
isolated CD8B, a human gene homologous to the gene encoding the second
rodent CD8 chain, and showed that it is transcribed in human thymocytes
and in some acute T-cell leukemias.
MAPPING
By analysis of a panel of 14 human-rodent somatic cell hybrids, Spurr et
al. (1988) found that CD8B maps to human chromosome 2. In the mouse, the
genes that encode the 2 CD8 chains are closely linked on chromosome 6.
No recombination was observed in 370 meiotic events. These genes are
also closely linked to the immunoglobulin kappa light chain cluster in
the mouse. The linkage between CD8A (186910) and the kappa locus is also
conserved in man. Close linkage is of interest since these proteins all
belong to the immunoglobulin superfamily.
Nakayama et al. (1992) discovered a second but inactive CD8 beta-chain
gene, which they named CD8B2, the functional gene being renamed CD8B1.
The nucleotide sequences of the coding and noncoding regions of CD8B1
and CD8B2 were found to be 98.9% homologous, suggesting that the
duplication event had occurred rather recently in the evolution of the
human lineage. They showed that the CD8B pseudogene was present in all
human Japanese and Caucasian samples tested. Delarbre et al. (1993)
showed that the pseudogene is missing exons 8 and 9 of CD8B. From a
study of somatic cell hybrids, they concluded that, like CD8A and CD8B,
the pseudogene is located on chromosome 2. Pulsed-field gel
electrophoresis experiments demonstrated that the pseudogene is at least
1,500 kb removed from the functional gene, CD8B.
Zhang et al. (1996) demonstrated, by fluorescence in situ hybridization,
that the second CD8B gene locus in the human maps to 2q12, a position on
the opposite side of the centromere from the first locus.
EVOLUTION
Delarbre et al. (1993) demonstrated that the duplication of the CD8B
gene had taken place in an ancestor common to the human, gorilla, and
chimpanzee clade, most likely excluding the orangutan and other
primates.
GENE FUNCTION
To locate factors regulating the expression of CD8 and the functional
commitment of CD8-positive T cells, Kieffer et al. (2002) identified
SATB1 (602075)- and GATA3 (131320)-binding sites in matrix attachment
regions and DNase I hypersensitive sites at the 3-prime end of the CD8B
gene. They proposed that this region is an epigenetic regulator of CD8
expression.
In mice, at least 5 Cd8 cis-acting enhancers, individually or in
combination, direct expression in the T-cell lineage, and deletion of
specific enhancers leads to variegated expression of Cd8a/Cd8b
heterodimers in double-positive thymocytes. Bilic et al. (2006) showed
that Cd8 variegation due to enhancer deletion correlated with an
epigenetic 'off' state, linking Cd8 enhancer function with chromatin
remodeling of Cd8a and Cd8b. The zinc finger protein Mazr (ZNF278;
605165) bound the Cd8 enhancer and, via its N-terminal domain,
interacted with the Ncor1 (600849) complex in double-negative
thymocytes. Mazr was downregulated in double-positive and Cd8
single-positive thymocytes. Constitutive expression of Mazr during
T-cell development resulted in variegated expression of Cd8 in
double-positive thymocytes. Bilic et al. (2006) concluded that MAZR is a
negative regulator of CD8 expression in double-negative thymocytes.
*FIELD* RF
1. Bilic, I.; Koesters, C.; Unger, B.; Sekimata, M.; Hertweck, A.;
Maschek, R.; Wilson, C. B.; Ellmeier, W.: Negative regulation of
CD8 expression via Cd8 enhancer-mediated recruitment of the zinc finger
protein MAZR. Nature Immun. 7: 392-400, 2006.
2. Delarbre, C.; Nakauchi, H.; Bontrop, R.; Kourilsky, P.; Gachelin,
G.: Duplication of the CD8 beta-chain gene as a marker of the man-gorilla-chimpanzee
clade. Proc. Nat. Acad. Sci. 90: 7049-7053, 1993.
3. Johnson, P.: A human homolog of the mouse CD8 molecule, Lyt-3:
genomic sequence and expression. Immunogenetics 26: 174-177, 1987.
4. Kieffer, L. J.; Greally, J. M.; Landres, I.; Nag, S.; Nakajima,
Y.; Kohwi-Shigematsu, T.; Kavathas, P. B.: Identification of a candidate
regulatory region in the human CD8 gene complex by colocalization
of DNase I hypersensitive sites and matrix attachment regions which
bind SATB1 and GATA-3. J. Immun. 168: 3915-3922, 2002.
5. Nakayama, K. I.; Kawachi, Y.; Tokito, S.; Minami, N.; Yamamoto,
R.; Imai, T.; Gachelin, G.; Nakauchi, H.: Recent duplication of the
two human CD8 beta-chain genes. J. Immun. 148: 1919-1927, 1992.
6. Spurr, N. K.; Goodfellow, P. N.; Johnson, P.: CD8B, the human
equivalent of the mouse Ly-3 gene is localized on chromosome 2. Immunogenetics 27:
70-72, 1988.
7. Zhang, X.-L.; Heng, H. H. Q.; Yang, Y.; Tsui, L.-C.; Parnes, J.
R.; Chamberlain, J. W.: Chromosomal mapping of the second human CD8B
gene locus. Immunogenetics 43: 220-226, 1996.
*FIELD* CN
Paul J. Converse - updated: 2/16/2007
Paul J. Converse - updated: 5/16/2002
*FIELD* CD
Victor A. McKusick: 2/12/1988
*FIELD* ED
carol: 07/30/2010
carol: 7/30/2010
mgross: 2/16/2007
mgross: 5/16/2002
dkim: 7/23/1998
mark: 3/28/1996
terry: 3/20/1996
carol: 10/12/1993
carol: 10/4/1993
supermim: 3/16/1992
supermim: 3/20/1990
ddp: 10/27/1989
carol: 6/19/1989