Full text data of CIAPIN1
CIAPIN1
[Confidence: low (only semi-automatic identification from reviews)]
Anamorsin (Cytokine-induced apoptosis inhibitor 1; Fe-S cluster assembly protein DRE2 homolog)
Anamorsin (Cytokine-induced apoptosis inhibitor 1; Fe-S cluster assembly protein DRE2 homolog)
UniProt
Q6FI81
ID CPIN1_HUMAN Reviewed; 312 AA.
AC Q6FI81; A8K8B6; O75206; O75207; Q9H0W1; Q9P1L7;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
read moreDT 04-JAN-2005, sequence version 2.
DT 22-JAN-2014, entry version 91.
DE RecName: Full=Anamorsin;
DE AltName: Full=Cytokine-induced apoptosis inhibitor 1;
DE AltName: Full=Fe-S cluster assembly protein DRE2 homolog;
GN Name=CIAPIN1; ORFNames=CUA001, PRO0915;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Adrenal tumor;
RA Yang Y., Xu X., Gao G., Xiao H., Chen Z., Han Z.;
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=11230166; DOI=10.1101/gr.GR1547R;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H.,
RA Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N.,
RA Mewes H.-W., Ottenwaelder B., Obermaier B., Tampe J., Heubner D.,
RA Wambutt R., Korn B., Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and
RT analysis of 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Fetal liver;
RX PubMed=11483580; DOI=10.1101/gr.175501;
RA Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C.,
RA Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C.,
RA He F.;
RT "Gene expression profiling in human fetal liver and identification of
RT tissue- and developmental-stage-specific genes through compiled
RT expression profiles and efficient cloning of full-length cDNAs.";
RL Genome Res. 11:1392-1403(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10493829; DOI=10.1006/geno.1999.5927;
RA Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R.,
RA Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L.,
RA Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E.,
RA Harris P.C., Venter J.C., Adams M.D.;
RT "Genome duplications and other features in 12 Mb of DNA sequence from
RT human chromosome 16p and 16q.";
RL Genomics 60:295-308(1999).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, Placenta, Skin, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=14970183; DOI=10.1084/jem.20031858;
RA Shibayama H., Takai E., Matsumura I., Kouno M., Morii E., Kitamura Y.,
RA Takeda J., Kanakura Y.;
RT "Identification of a cytokine-induced antiapoptotic molecule anamorsin
RT essential for definitive hematopoiesis.";
RL J. Exp. Med. 199:581-592(2004).
RN [10]
RP SUBCELLULAR LOCATION.
RX PubMed=16957168; DOI=10.1369/jhc.6A6960.2006;
RA Hao Z., Li X., Qiao T., Du R., Zhang G., Fan D.;
RT "Subcellular localization of CIAPIN1.";
RL J. Histochem. Cytochem. 54:1437-1444(2006).
RN [11]
RP LACK OF METHYLTRANSFERASE ACTIVITY.
RX PubMed=17935775; DOI=10.1016/j.ijbiomac.2007.08.009;
RA Hao Z., Li X., Qiao T., Fan D.;
RT "Successful expression and purification of human CIAPIN1 in
RT baculovirus-insect cell system and application of this system to
RT investigation of its potential methyltransferase activity.";
RL Int. J. Biol. Macromol. 42:27-32(2008).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182 AND SER-183, AND
RP MASS SPECTROMETRY.
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [14]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305 AND SER-307, AND
RP MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [15]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [16]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305, AND MASS
RP SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [17]
RP INTERACTION WITH NDOR1, DOMAIN, FUNCTION, AND COFACTOR.
RX PubMed=23596212; DOI=10.1073/pnas.1302378110;
RA Banci L., Bertini I., Calderone V., Ciofi-Baffoni S., Giachetti A.,
RA Jaiswal D., Mikolajczyk M., Piccioli M., Winkelmann J.;
RT "Molecular view of an electron transfer process essential for iron-
RT sulfur protein biogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7136-7141(2013).
RN [18]
RP STRUCTURE BY NMR OF 1-171.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the N-terminal domain in human cytokine-induced
RT apoptosis inhibitor anamorsin.";
RL Submitted (APR-2008) to the PDB data bank.
RN [19]
RP STRUCTURE BY NMR OF 1-172, COFACTOR, INTERACTION WITH CHCHD4, SUBUNIT,
RP MASS SPECTROMETRY, SUBCELLUALR LOCATION, AND DOMAIN.
RX PubMed=21700214; DOI=10.1016/j.chembiol.2011.03.015;
RA Banci L., Bertini I., Ciofi-Baffoni S., Boscaro F., Chatzi A.,
RA Mikolajczyk M., Tokatlidis K., Winkelmann J.;
RT "Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-
RT dependent mitochondrial protein trapping machinery.";
RL Chem. Biol. 18:794-804(2011).
CC -!- FUNCTION: Has anti-apoptotic effects in the cell. Involved in
CC negative control of cell death upon cytokine withdrawal. Promotes
CC development of hematopoietic cells (By similarity). Component of
CC the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.
CC Required for the maturation of extramitochondrial Fe-S proteins.
CC Part of an electron transfer chain functioning in an early step of
CC cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S
CC cluster from NADPH via the FAD- and FMN-containing protein NDOR1.
CC -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit.
CC -!- SUBUNIT: Monomer. Interacts with NDOR1; its oxidized form can be
CC reduced by NDOR1. Interacts with CHCHD4 and may be a substrate for
CC CHCHD4 chaperone activity.
CC -!- INTERACTION:
CC O76003:GLRX3; NbExp=3; IntAct=EBI-750511, EBI-374781;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Mitochondrion.
CC Mitochondrion intermembrane space (Probable). Note=Imported into
CC mitochondria (in vitro).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6FI81-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6FI81-2; Sequence=VSP_012360;
CC Note=No experimental confirmation available;
CC Name=3;
CC IsoId=Q6FI81-3; Sequence=VSP_012361;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed. Highly expressed in
CC heart, liver and pancreas.
CC -!- DOMAIN: The N-terminal domain has structural similarity with S-
CC adenosyl-L-methionine-dependent methyltransferases, but does not
CC bind S-adenosyl-L-methionine (PubMed:21700214).
CC -!- DOMAIN: The C-terminal domain binds one 2Fe-2S iron-sulfur cluster
CC but is otherwise mostly in an intrinsically disordered
CC conformation.
CC -!- DOMAIN: The twin Cx2C motifs are involved in the recognition by
CC the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system.
CC The formation of 2 disulfide bonds in the Cx2C motifs through
CC dithiol/disulfide exchange reactions effectively traps the protein
CC in the mitochondrial intermembrane space.
CC -!- MISCELLANEOUS: 'Ana-mors-in' means 'anti-death molecule' in Latin.
CC -!- SIMILARITY: Belongs to the anamorsin family.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24312.1; Type=Erroneous gene model prediction;
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DR EMBL; AF248964; AAG44562.1; -; mRNA.
DR EMBL; AL136613; CAB66548.1; -; mRNA.
DR EMBL; AF116609; AAF71034.1; -; mRNA.
DR EMBL; CR533545; CAG38576.1; -; mRNA.
DR EMBL; AK292281; BAF84970.1; -; mRNA.
DR EMBL; AC004382; AAC24311.1; -; Genomic_DNA.
DR EMBL; AC004382; AAC24312.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CH471092; EAW82923.1; -; Genomic_DNA.
DR EMBL; BC002568; AAH02568.1; -; mRNA.
DR EMBL; BC024196; AAH24196.1; -; mRNA.
DR EMBL; BC067303; AAH67303.1; -; mRNA.
DR EMBL; BC071740; AAH71740.1; -; mRNA.
DR RefSeq; NP_064709.2; NM_020313.2.
DR RefSeq; XP_005256118.1; XM_005256061.1.
DR UniGene; Hs.4900; -.
DR PDB; 2LD4; NMR; -; A=1-172.
DR PDB; 2YUI; NMR; -; A=1-170.
DR PDB; 4M7R; X-ray; 1.80 A; A/B=1-172.
DR PDBsum; 2LD4; -.
DR PDBsum; 2YUI; -.
DR PDBsum; 4M7R; -.
DR ProteinModelPortal; Q6FI81; -.
DR SMR; Q6FI81; 1-172.
DR IntAct; Q6FI81; 4.
DR MINT; MINT-1469842; -.
DR STRING; 9606.ENSP00000377914; -.
DR PhosphoSite; Q6FI81; -.
DR DMDM; 57012667; -.
DR PaxDb; Q6FI81; -.
DR PRIDE; Q6FI81; -.
DR DNASU; 57019; -.
DR Ensembl; ENST00000394391; ENSP00000377914; ENSG00000005194.
DR Ensembl; ENST00000567518; ENSP00000456114; ENSG00000005194.
DR GeneID; 57019; -.
DR KEGG; hsa:57019; -.
DR UCSC; uc002ell.1; human.
DR CTD; 57019; -.
DR GeneCards; GC16M057462; -.
DR HGNC; HGNC:28050; CIAPIN1.
DR HPA; HPA041350; -.
DR HPA; HPA042182; -.
DR MIM; 608943; gene.
DR neXtProt; NX_Q6FI81; -.
DR PharmGKB; PA134978864; -.
DR eggNOG; COG5636; -.
DR HOGENOM; HOG000241118; -.
DR HOVERGEN; HBG051104; -.
DR OMA; DSMDLID; -.
DR OrthoDB; EOG7WHHC3; -.
DR Reactome; REACT_111217; Metabolism.
DR ChiTaRS; CIAPIN1; human.
DR EvolutionaryTrace; Q6FI81; -.
DR GeneWiki; CIAPIN1; -.
DR GenomeRNAi; 57019; -.
DR NextBio; 62770; -.
DR PRO; PR:Q6FI81; -.
DR ArrayExpress; Q6FI81; -.
DR Bgee; Q6FI81; -.
DR CleanEx; HS_CIAPIN1; -.
DR Genevestigator; Q6FI81; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0009055; F:electron carrier activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0030097; P:hemopoiesis; IEA:UniProtKB-HAMAP.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:UniProtKB-HAMAP.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR HAMAP; MF_03115; Anamorsin; 1; -.
DR InterPro; IPR007785; Anamorsin.
DR PANTHER; PTHR13273; PTHR13273; 1.
DR Pfam; PF05093; CIAPIN1; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; Alternative splicing; Apoptosis;
KW Complete proteome; Cytoplasm; Iron; Iron-sulfur; Metal-binding;
KW Mitochondrion; Nucleus; Phosphoprotein; Polymorphism;
KW Reference proteome.
FT CHAIN 1 312 Anamorsin.
FT /FTId=PRO_0000079288.
FT REGION 1 172 N-terminal domain.
FT REGION 173 223 Linker.
FT REGION 224 312 Intrinsically disordered.
FT MOTIF 274 277 Cx2C motif 1.
FT MOTIF 285 288 Cx2C motif 2.
FT METAL 237 237 Iron-sulfur (2Fe-2S).
FT METAL 246 246 Iron-sulfur (2Fe-2S).
FT METAL 249 249 Iron-sulfur (2Fe-2S).
FT METAL 251 251 Iron-sulfur (2Fe-2S).
FT MOD_RES 182 182 Phosphoserine.
FT MOD_RES 183 183 Phosphoserine.
FT MOD_RES 305 305 Phosphoserine.
FT MOD_RES 307 307 Phosphoserine.
FT VAR_SEQ 1 204 Missing (in isoform 2).
FT /FTId=VSP_012360.
FT VAR_SEQ 53 66 SAHKESSFDIILSG -> C (in isoform 3).
FT /FTId=VSP_012361.
FT VARIANT 34 34 A -> E (in dbSNP:rs11557672).
FT /FTId=VAR_033747.
FT VARIANT 52 52 Q -> E (in dbSNP:rs11557674).
FT /FTId=VAR_033748.
FT CONFLICT 142 142 S -> F (in Ref. 2; CAB66548 and 4;
FT CAG38576).
FT CONFLICT 278 278 Y -> C (in Ref. 4; CAG38576).
FT CONFLICT 289 289 P -> T (in Ref. 2; CAB66548 and 4;
FT CAG38576).
FT CONFLICT 310 310 H -> N (in Ref. 2; CAB66548 and 4;
FT CAG38576).
FT TURN 2 5
FT STRAND 10 16
FT HELIX 22 35
FT TURN 36 39
FT STRAND 40 46
FT HELIX 47 53
FT STRAND 60 65
FT STRAND 68 70
FT HELIX 77 86
FT STRAND 87 103
FT STRAND 105 109
FT HELIX 112 121
FT STRAND 125 133
FT HELIX 137 146
FT STRAND 152 162
SQ SEQUENCE 312 AA; 33582 MW; 70A71D44074ABFBA CRC64;
MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL LQSAHKESSF
DIILSGLVPG STTLHSAEIL AEIARILRPG GCLFLKEPVE TAVDNNSKVK TASKLCSALT
LSGLVEVKEL QREPLTPEEV QSVREHLGHE SDNLLFVQIT GKKPNFEVGS SRQLKLSITK
KSSPSVKPAV DPAAAKLWTL SANDMEDDSM DLIDSDELLD PEDLKKPDPA SLRAASCGEG
KKRKACKNCT CGLAEELEKE KSREQMSSQP KSACGNCYLG DAFRCASCPY LGMPAFKPGE
KVLLSDSNLH DA
//
ID CPIN1_HUMAN Reviewed; 312 AA.
AC Q6FI81; A8K8B6; O75206; O75207; Q9H0W1; Q9P1L7;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
read moreDT 04-JAN-2005, sequence version 2.
DT 22-JAN-2014, entry version 91.
DE RecName: Full=Anamorsin;
DE AltName: Full=Cytokine-induced apoptosis inhibitor 1;
DE AltName: Full=Fe-S cluster assembly protein DRE2 homolog;
GN Name=CIAPIN1; ORFNames=CUA001, PRO0915;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Adrenal tumor;
RA Yang Y., Xu X., Gao G., Xiao H., Chen Z., Han Z.;
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=11230166; DOI=10.1101/gr.GR1547R;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H.,
RA Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N.,
RA Mewes H.-W., Ottenwaelder B., Obermaier B., Tampe J., Heubner D.,
RA Wambutt R., Korn B., Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and
RT analysis of 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Fetal liver;
RX PubMed=11483580; DOI=10.1101/gr.175501;
RA Yu Y., Zhang C., Zhou G., Wu S., Qu X., Wei H., Xing G., Dong C.,
RA Zhai Y., Wan J., Ouyang S., Li L., Zhang S., Zhou K., Zhang Y., Wu C.,
RA He F.;
RT "Gene expression profiling in human fetal liver and identification of
RT tissue- and developmental-stage-specific genes through compiled
RT expression profiles and efficient cloning of full-length cDNAs.";
RL Genome Res. 11:1392-1403(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10493829; DOI=10.1006/geno.1999.5927;
RA Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R.,
RA Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L.,
RA Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E.,
RA Harris P.C., Venter J.C., Adams M.D.;
RT "Genome duplications and other features in 12 Mb of DNA sequence from
RT human chromosome 16p and 16q.";
RL Genomics 60:295-308(1999).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, Placenta, Skin, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP TISSUE SPECIFICITY.
RX PubMed=14970183; DOI=10.1084/jem.20031858;
RA Shibayama H., Takai E., Matsumura I., Kouno M., Morii E., Kitamura Y.,
RA Takeda J., Kanakura Y.;
RT "Identification of a cytokine-induced antiapoptotic molecule anamorsin
RT essential for definitive hematopoiesis.";
RL J. Exp. Med. 199:581-592(2004).
RN [10]
RP SUBCELLULAR LOCATION.
RX PubMed=16957168; DOI=10.1369/jhc.6A6960.2006;
RA Hao Z., Li X., Qiao T., Du R., Zhang G., Fan D.;
RT "Subcellular localization of CIAPIN1.";
RL J. Histochem. Cytochem. 54:1437-1444(2006).
RN [11]
RP LACK OF METHYLTRANSFERASE ACTIVITY.
RX PubMed=17935775; DOI=10.1016/j.ijbiomac.2007.08.009;
RA Hao Z., Li X., Qiao T., Fan D.;
RT "Successful expression and purification of human CIAPIN1 in
RT baculovirus-insect cell system and application of this system to
RT investigation of its potential methyltransferase activity.";
RL Int. J. Biol. Macromol. 42:27-32(2008).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-307, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182 AND SER-183, AND
RP MASS SPECTROMETRY.
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [14]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305 AND SER-307, AND
RP MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [15]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [16]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-305, AND MASS
RP SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [17]
RP INTERACTION WITH NDOR1, DOMAIN, FUNCTION, AND COFACTOR.
RX PubMed=23596212; DOI=10.1073/pnas.1302378110;
RA Banci L., Bertini I., Calderone V., Ciofi-Baffoni S., Giachetti A.,
RA Jaiswal D., Mikolajczyk M., Piccioli M., Winkelmann J.;
RT "Molecular view of an electron transfer process essential for iron-
RT sulfur protein biogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:7136-7141(2013).
RN [18]
RP STRUCTURE BY NMR OF 1-171.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the N-terminal domain in human cytokine-induced
RT apoptosis inhibitor anamorsin.";
RL Submitted (APR-2008) to the PDB data bank.
RN [19]
RP STRUCTURE BY NMR OF 1-172, COFACTOR, INTERACTION WITH CHCHD4, SUBUNIT,
RP MASS SPECTROMETRY, SUBCELLUALR LOCATION, AND DOMAIN.
RX PubMed=21700214; DOI=10.1016/j.chembiol.2011.03.015;
RA Banci L., Bertini I., Ciofi-Baffoni S., Boscaro F., Chatzi A.,
RA Mikolajczyk M., Tokatlidis K., Winkelmann J.;
RT "Anamorsin is a [2Fe-2S] cluster-containing substrate of the Mia40-
RT dependent mitochondrial protein trapping machinery.";
RL Chem. Biol. 18:794-804(2011).
CC -!- FUNCTION: Has anti-apoptotic effects in the cell. Involved in
CC negative control of cell death upon cytokine withdrawal. Promotes
CC development of hematopoietic cells (By similarity). Component of
CC the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.
CC Required for the maturation of extramitochondrial Fe-S proteins.
CC Part of an electron transfer chain functioning in an early step of
CC cytosolic Fe-S biogenesis. Electrons are transferred to the Fe-S
CC cluster from NADPH via the FAD- and FMN-containing protein NDOR1.
CC -!- COFACTOR: Binds 1 2Fe-2S cluster per subunit.
CC -!- SUBUNIT: Monomer. Interacts with NDOR1; its oxidized form can be
CC reduced by NDOR1. Interacts with CHCHD4 and may be a substrate for
CC CHCHD4 chaperone activity.
CC -!- INTERACTION:
CC O76003:GLRX3; NbExp=3; IntAct=EBI-750511, EBI-374781;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Mitochondrion.
CC Mitochondrion intermembrane space (Probable). Note=Imported into
CC mitochondria (in vitro).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6FI81-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6FI81-2; Sequence=VSP_012360;
CC Note=No experimental confirmation available;
CC Name=3;
CC IsoId=Q6FI81-3; Sequence=VSP_012361;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed. Highly expressed in
CC heart, liver and pancreas.
CC -!- DOMAIN: The N-terminal domain has structural similarity with S-
CC adenosyl-L-methionine-dependent methyltransferases, but does not
CC bind S-adenosyl-L-methionine (PubMed:21700214).
CC -!- DOMAIN: The C-terminal domain binds one 2Fe-2S iron-sulfur cluster
CC but is otherwise mostly in an intrinsically disordered
CC conformation.
CC -!- DOMAIN: The twin Cx2C motifs are involved in the recognition by
CC the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system.
CC The formation of 2 disulfide bonds in the Cx2C motifs through
CC dithiol/disulfide exchange reactions effectively traps the protein
CC in the mitochondrial intermembrane space.
CC -!- MISCELLANEOUS: 'Ana-mors-in' means 'anti-death molecule' in Latin.
CC -!- SIMILARITY: Belongs to the anamorsin family.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24312.1; Type=Erroneous gene model prediction;
CC -----------------------------------------------------------------------
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DR EMBL; AF248964; AAG44562.1; -; mRNA.
DR EMBL; AL136613; CAB66548.1; -; mRNA.
DR EMBL; AF116609; AAF71034.1; -; mRNA.
DR EMBL; CR533545; CAG38576.1; -; mRNA.
DR EMBL; AK292281; BAF84970.1; -; mRNA.
DR EMBL; AC004382; AAC24311.1; -; Genomic_DNA.
DR EMBL; AC004382; AAC24312.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CH471092; EAW82923.1; -; Genomic_DNA.
DR EMBL; BC002568; AAH02568.1; -; mRNA.
DR EMBL; BC024196; AAH24196.1; -; mRNA.
DR EMBL; BC067303; AAH67303.1; -; mRNA.
DR EMBL; BC071740; AAH71740.1; -; mRNA.
DR RefSeq; NP_064709.2; NM_020313.2.
DR RefSeq; XP_005256118.1; XM_005256061.1.
DR UniGene; Hs.4900; -.
DR PDB; 2LD4; NMR; -; A=1-172.
DR PDB; 2YUI; NMR; -; A=1-170.
DR PDB; 4M7R; X-ray; 1.80 A; A/B=1-172.
DR PDBsum; 2LD4; -.
DR PDBsum; 2YUI; -.
DR PDBsum; 4M7R; -.
DR ProteinModelPortal; Q6FI81; -.
DR SMR; Q6FI81; 1-172.
DR IntAct; Q6FI81; 4.
DR MINT; MINT-1469842; -.
DR STRING; 9606.ENSP00000377914; -.
DR PhosphoSite; Q6FI81; -.
DR DMDM; 57012667; -.
DR PaxDb; Q6FI81; -.
DR PRIDE; Q6FI81; -.
DR DNASU; 57019; -.
DR Ensembl; ENST00000394391; ENSP00000377914; ENSG00000005194.
DR Ensembl; ENST00000567518; ENSP00000456114; ENSG00000005194.
DR GeneID; 57019; -.
DR KEGG; hsa:57019; -.
DR UCSC; uc002ell.1; human.
DR CTD; 57019; -.
DR GeneCards; GC16M057462; -.
DR HGNC; HGNC:28050; CIAPIN1.
DR HPA; HPA041350; -.
DR HPA; HPA042182; -.
DR MIM; 608943; gene.
DR neXtProt; NX_Q6FI81; -.
DR PharmGKB; PA134978864; -.
DR eggNOG; COG5636; -.
DR HOGENOM; HOG000241118; -.
DR HOVERGEN; HBG051104; -.
DR OMA; DSMDLID; -.
DR OrthoDB; EOG7WHHC3; -.
DR Reactome; REACT_111217; Metabolism.
DR ChiTaRS; CIAPIN1; human.
DR EvolutionaryTrace; Q6FI81; -.
DR GeneWiki; CIAPIN1; -.
DR GenomeRNAi; 57019; -.
DR NextBio; 62770; -.
DR PRO; PR:Q6FI81; -.
DR ArrayExpress; Q6FI81; -.
DR Bgee; Q6FI81; -.
DR CleanEx; HS_CIAPIN1; -.
DR Genevestigator; Q6FI81; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-HAMAP.
DR GO; GO:0009055; F:electron carrier activity; IEA:UniProtKB-HAMAP.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0030097; P:hemopoiesis; IEA:UniProtKB-HAMAP.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:UniProtKB-HAMAP.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR HAMAP; MF_03115; Anamorsin; 1; -.
DR InterPro; IPR007785; Anamorsin.
DR PANTHER; PTHR13273; PTHR13273; 1.
DR Pfam; PF05093; CIAPIN1; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; 3D-structure; Alternative splicing; Apoptosis;
KW Complete proteome; Cytoplasm; Iron; Iron-sulfur; Metal-binding;
KW Mitochondrion; Nucleus; Phosphoprotein; Polymorphism;
KW Reference proteome.
FT CHAIN 1 312 Anamorsin.
FT /FTId=PRO_0000079288.
FT REGION 1 172 N-terminal domain.
FT REGION 173 223 Linker.
FT REGION 224 312 Intrinsically disordered.
FT MOTIF 274 277 Cx2C motif 1.
FT MOTIF 285 288 Cx2C motif 2.
FT METAL 237 237 Iron-sulfur (2Fe-2S).
FT METAL 246 246 Iron-sulfur (2Fe-2S).
FT METAL 249 249 Iron-sulfur (2Fe-2S).
FT METAL 251 251 Iron-sulfur (2Fe-2S).
FT MOD_RES 182 182 Phosphoserine.
FT MOD_RES 183 183 Phosphoserine.
FT MOD_RES 305 305 Phosphoserine.
FT MOD_RES 307 307 Phosphoserine.
FT VAR_SEQ 1 204 Missing (in isoform 2).
FT /FTId=VSP_012360.
FT VAR_SEQ 53 66 SAHKESSFDIILSG -> C (in isoform 3).
FT /FTId=VSP_012361.
FT VARIANT 34 34 A -> E (in dbSNP:rs11557672).
FT /FTId=VAR_033747.
FT VARIANT 52 52 Q -> E (in dbSNP:rs11557674).
FT /FTId=VAR_033748.
FT CONFLICT 142 142 S -> F (in Ref. 2; CAB66548 and 4;
FT CAG38576).
FT CONFLICT 278 278 Y -> C (in Ref. 4; CAG38576).
FT CONFLICT 289 289 P -> T (in Ref. 2; CAB66548 and 4;
FT CAG38576).
FT CONFLICT 310 310 H -> N (in Ref. 2; CAB66548 and 4;
FT CAG38576).
FT TURN 2 5
FT STRAND 10 16
FT HELIX 22 35
FT TURN 36 39
FT STRAND 40 46
FT HELIX 47 53
FT STRAND 60 65
FT STRAND 68 70
FT HELIX 77 86
FT STRAND 87 103
FT STRAND 105 109
FT HELIX 112 121
FT STRAND 125 133
FT HELIX 137 146
FT STRAND 152 162
SQ SEQUENCE 312 AA; 33582 MW; 70A71D44074ABFBA CRC64;
MADFGISAGQ FVAVVWDKSS PVEALKGLVD KLQALTGNEG RVSVENIKQL LQSAHKESSF
DIILSGLVPG STTLHSAEIL AEIARILRPG GCLFLKEPVE TAVDNNSKVK TASKLCSALT
LSGLVEVKEL QREPLTPEEV QSVREHLGHE SDNLLFVQIT GKKPNFEVGS SRQLKLSITK
KSSPSVKPAV DPAAAKLWTL SANDMEDDSM DLIDSDELLD PEDLKKPDPA SLRAASCGEG
KKRKACKNCT CGLAEELEKE KSREQMSSQP KSACGNCYLG DAFRCASCPY LGMPAFKPGE
KVLLSDSNLH DA
//
MIM
608943
*RECORD*
*FIELD* NO
608943
*FIELD* TI
*608943 CYTOKINE-INDUCED APOPTOSIS INHIBITOR 1; CIAPIN1
;;ANAMORSIN
*FIELD* TX
DESCRIPTION
read more
CIAPIN1 is a cytokine-induced inhibitor of apoptosis with no relation to
apoptosis regulatory molecules of the BCL2 (151430) or CASP (see 147678)
families. Expression of CIAPIN1 is dependent on growth factor
stimulation (Shibayama et al., 2004).
CLONING
Using expression cloning with an IL3 (147740)-independent subline of an
IL3-dependent mouse cell line, Shibayama et al. (2004) obtained a cDNA
encoding Ciapin1, which they termed Anamorsin, meaning 'anti-death
molecule' in Latin. By searching databases, Shibayama et al. (2004)
identified human Anamorsin. The predicted 310-amino acid mouse protein
has a calculated molecular mass of 37 kD and contains an N-terminal
methyltransferase motif. Human and mouse Anamorsin share 82% amino acid
identity. Northern blot analysis of human tissues revealed ubiquitous
expression of Anamorsin, with particularly high levels in heart, liver,
and pancreas. Immunofluorescence microscopy of mouse cells demonstrated
that Anamorsin was localized in the cytoplasm exclusively.
GENE FUNCTION
Shibayama et al. (2004) found that expression of Anamorsin in mouse
cells conferred resistance to apoptosis caused by IL3 deprivation.
Addition of growth factors, such as EPO (133170), SCF (184745), TPO
(600044), or IL3, all of which depend on RAS (190020) signaling, induced
dose-dependent expression of Anamorsin in mouse cells. Based on these
results and their findings in Anamorsin null mice, Shibayama et al.
(2004) concluded that Anamorsin is critical in hematopoiesis and in the
mediation of cytokine-induced antiapoptosis.
MAPPING
Loftus et al. (1999) identified the CIAPIN1 gene on chromosome 16.
ANIMAL MODEL
Shibayama et al. (2004) generated Anamorsin null mice, all of which died
by late gestation due to a hematopoiesis defect in fetal liver. Myeloid,
and particularly erythroid, colony formation was disrupted in these
mice. Microarray analysis revealed reduced expression of Bclxl (600039)
and Jak2 (147796) in fetal liver of Anamorsin null mice.
*FIELD* RF
1. Loftus, B. J.; Kim, U.-J.; Sneddon, V. P.; Kalush, F.; Brandon,
R.; Fuhrmann, J.; Mason, T.; Crosby, M. L.; Barnstead, M.; Cronin,
L.; Deslattes Mays, A.; Cao, Y.; Xu, R. X.; Kang, H.-L.; Mitchell,
S.; Eichler, E. E.; Harris, P. C.; Venter, J. C.; Adams, M. D.: Genome
duplications and other features in 12 Mb of DNA sequence from human
chromosome 16p and 16q. Genomics 60: 295-308, 1999.
2. Shibayama, H.; Takai, E.; Matsumura, I.; Kouno, M.; Morii, E.;
Kitamura, Y.; Takeda, J.; Kanakura, Y.: Identification of a cytokine-induced
antiapoptotic molecule Anamorsin essential for definitive hematopoiesis. J.
Exp. Med. 199: 581-592, 2004.
*FIELD* CD
Paul J. Converse: 9/27/2004
*FIELD* ED
terry: 11/02/2004
mgross: 9/27/2004
*RECORD*
*FIELD* NO
608943
*FIELD* TI
*608943 CYTOKINE-INDUCED APOPTOSIS INHIBITOR 1; CIAPIN1
;;ANAMORSIN
*FIELD* TX
DESCRIPTION
read more
CIAPIN1 is a cytokine-induced inhibitor of apoptosis with no relation to
apoptosis regulatory molecules of the BCL2 (151430) or CASP (see 147678)
families. Expression of CIAPIN1 is dependent on growth factor
stimulation (Shibayama et al., 2004).
CLONING
Using expression cloning with an IL3 (147740)-independent subline of an
IL3-dependent mouse cell line, Shibayama et al. (2004) obtained a cDNA
encoding Ciapin1, which they termed Anamorsin, meaning 'anti-death
molecule' in Latin. By searching databases, Shibayama et al. (2004)
identified human Anamorsin. The predicted 310-amino acid mouse protein
has a calculated molecular mass of 37 kD and contains an N-terminal
methyltransferase motif. Human and mouse Anamorsin share 82% amino acid
identity. Northern blot analysis of human tissues revealed ubiquitous
expression of Anamorsin, with particularly high levels in heart, liver,
and pancreas. Immunofluorescence microscopy of mouse cells demonstrated
that Anamorsin was localized in the cytoplasm exclusively.
GENE FUNCTION
Shibayama et al. (2004) found that expression of Anamorsin in mouse
cells conferred resistance to apoptosis caused by IL3 deprivation.
Addition of growth factors, such as EPO (133170), SCF (184745), TPO
(600044), or IL3, all of which depend on RAS (190020) signaling, induced
dose-dependent expression of Anamorsin in mouse cells. Based on these
results and their findings in Anamorsin null mice, Shibayama et al.
(2004) concluded that Anamorsin is critical in hematopoiesis and in the
mediation of cytokine-induced antiapoptosis.
MAPPING
Loftus et al. (1999) identified the CIAPIN1 gene on chromosome 16.
ANIMAL MODEL
Shibayama et al. (2004) generated Anamorsin null mice, all of which died
by late gestation due to a hematopoiesis defect in fetal liver. Myeloid,
and particularly erythroid, colony formation was disrupted in these
mice. Microarray analysis revealed reduced expression of Bclxl (600039)
and Jak2 (147796) in fetal liver of Anamorsin null mice.
*FIELD* RF
1. Loftus, B. J.; Kim, U.-J.; Sneddon, V. P.; Kalush, F.; Brandon,
R.; Fuhrmann, J.; Mason, T.; Crosby, M. L.; Barnstead, M.; Cronin,
L.; Deslattes Mays, A.; Cao, Y.; Xu, R. X.; Kang, H.-L.; Mitchell,
S.; Eichler, E. E.; Harris, P. C.; Venter, J. C.; Adams, M. D.: Genome
duplications and other features in 12 Mb of DNA sequence from human
chromosome 16p and 16q. Genomics 60: 295-308, 1999.
2. Shibayama, H.; Takai, E.; Matsumura, I.; Kouno, M.; Morii, E.;
Kitamura, Y.; Takeda, J.; Kanakura, Y.: Identification of a cytokine-induced
antiapoptotic molecule Anamorsin essential for definitive hematopoiesis. J.
Exp. Med. 199: 581-592, 2004.
*FIELD* CD
Paul J. Converse: 9/27/2004
*FIELD* ED
terry: 11/02/2004
mgross: 9/27/2004