Full text data of HSD17B11
HSD17B11
(DHRS8, PAN1B)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Estradiol 17-beta-dehydrogenase 11; 1.1.1.62 (17-beta-hydroxysteroid dehydrogenase 11; 17-beta-HSD 11; 17bHSD11; 17betaHSD11; 17-beta-hydroxysteroid dehydrogenase XI; 17-beta-HSD XI; 17betaHSDXI; Cutaneous T-cell lymphoma-associated antigen HD-CL-03; CTCL-associated antigen HD-CL-03; Dehydrogenase/reductase SDR family member 8; Retinal short-chain dehydrogenase/reductase 2; retSDR2; Flags: Precursor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Estradiol 17-beta-dehydrogenase 11; 1.1.1.62 (17-beta-hydroxysteroid dehydrogenase 11; 17-beta-HSD 11; 17bHSD11; 17betaHSD11; 17-beta-hydroxysteroid dehydrogenase XI; 17-beta-HSD XI; 17betaHSDXI; Cutaneous T-cell lymphoma-associated antigen HD-CL-03; CTCL-associated antigen HD-CL-03; Dehydrogenase/reductase SDR family member 8; Retinal short-chain dehydrogenase/reductase 2; retSDR2; Flags: Precursor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
Q8NBQ5
ID DHB11_HUMAN Reviewed; 300 AA.
AC Q8NBQ5; Q96HF6; Q9UKU4;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
read moreDT 18-MAY-2010, sequence version 3.
DT 22-JAN-2014, entry version 104.
DE RecName: Full=Estradiol 17-beta-dehydrogenase 11;
DE EC=1.1.1.62;
DE AltName: Full=17-beta-hydroxysteroid dehydrogenase 11;
DE Short=17-beta-HSD 11;
DE Short=17bHSD11;
DE Short=17betaHSD11;
DE AltName: Full=17-beta-hydroxysteroid dehydrogenase XI;
DE Short=17-beta-HSD XI;
DE Short=17betaHSDXI;
DE AltName: Full=Cutaneous T-cell lymphoma-associated antigen HD-CL-03;
DE Short=CTCL-associated antigen HD-CL-03;
DE AltName: Full=Dehydrogenase/reductase SDR family member 8;
DE AltName: Full=Retinal short-chain dehydrogenase/reductase 2;
DE Short=retSDR2;
DE Flags: Precursor;
GN Name=HSD17B11; Synonyms=DHRS8, PAN1B;
GN ORFNames=PSEC0029, UNQ207/PRO233;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Retina;
RX PubMed=10800688; DOI=10.1016/S0076-6879(00)16736-9;
RA Haeseleer F., Palczewski K.;
RT "Short-chain dehydrogenases/reductases in retina.";
RL Methods Enzymol. 316:372-383(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lymphoma;
RX PubMed=14996095; DOI=10.1111/j.1365-2133.2004.05651.x;
RA Hartmann T.B., Thiel D., Dummer R., Schadendorf D., Eichmueller S.;
RT "SEREX identification of new tumour-associated antigens in cutaneous
RT T-cell lymphoma.";
RL Br. J. Dermatol. 150:252-258(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Teratocarcinoma;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-
RT length human cDNAs encoding secretion or membrane proteins from oligo-
RT capped cDNA libraries.";
RL DNA Res. 12:117-126(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow, Colon, Kidney, Liver, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=9888557;
RA Li K.X.Z., Smith R.E., Krozowski Z.S.;
RT "Cloning and expression of a novel tissue specific 17beta-
RT hydroxysteroid dehydrogenase.";
RL Endocr. Res. 24:663-667(1998).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=11165019; DOI=10.1016/S0303-7207(00)00417-2;
RA Brereton P., Suzuki T., Sasano H., Li K., Duarte C., Obeyesekere V.,
RA Haeseleer F., Palczewski K., Smith I., Komesaroff P., Krozowski Z.;
RT "Pan1b (17betaHSD11)-enzymatic activity and distribution in the
RT lung.";
RL Mol. Cell. Endocrinol. 171:111-117(2001).
RN [9]
RP ENZYME ACTIVITY IN VITRO, AND TISSUE SPECIFICITY.
RX PubMed=12697717; DOI=10.1210/en.2002-221030;
RA Chai Z., Brereton P., Suzuki T., Sasano H., Obeyesekere V., Escher G.,
RA Saffery R., Fuller P., Enriquez C., Krozowski Z.;
RT "17 beta-hydroxysteroid dehydrogenase type XI localizes to human
RT steroidogenic cells.";
RL Endocrinology 144:2084-2091(2003).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 28-275.
RA Lukacik P., Bunkoczi G., Kavanagh K., Ng S., Von delft F., Bray J.,
RA Edwards A., Arrowsmith C., Sundstrom M., Oppermann U.;
RT "Crystal structure of human 17-beta-hydroxysteroid dehydrogenase type
RT XI.";
RL Submitted (DEC-2004) to the PDB data bank.
CC -!- FUNCTION: Can convert androstan-3-alpha,17-beta-diol (3-alpha-
CC diol) to androsterone in vitro, suggesting that it may participate
CC in androgen metabolism during steroidogenesis. May act by
CC metabolizing compounds that stimulate steroid synthesis and/or by
CC generating metabolites that inhibit it. Has no activity toward
CC DHEA (dehydroepiandrosterone), or A-dione (4-androste-3,17-dione),
CC and only a slight activity toward testosterone to A-dione. Tumor-
CC associated antigen in cutaneous T-cell lymphoma.
CC -!- CATALYTIC ACTIVITY: 17-beta-estradiol + NAD(P)(+) = estrone +
CC NAD(P)H.
CC -!- SUBCELLULAR LOCATION: Secreted (Potential).
CC -!- TISSUE SPECIFICITY: Present at high level in steroidogenic cells
CC such as syncytiotrophoblasts, sebaceous gland, Leydig cells, and
CC granulosa cells of the dominant follicle and corpus luteum. In
CC lung, it is detected in the ciliated epithelium and in acini of
CC adult trachea, in bronchioles, but not in alveoli. In the eye, it
CC is detected in the nonpigmented epithelium of the ciliary body
CC and, at lower level, in the inner nuclear layer of the retina (at
CC protein level). Widely expressed. Highly expressed in retina,
CC pancreas, kidney, liver, lung, adrenal, small intestine, ovary and
CC heart.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC (SDR) family. 17-beta-HSD 3 subfamily.
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DR EMBL; AF126780; AAF06939.1; -; mRNA.
DR EMBL; AF273056; AAM44459.1; -; mRNA.
DR EMBL; AY358553; AAQ88917.1; -; mRNA.
DR EMBL; AK075348; BAC11560.1; -; mRNA.
DR EMBL; AC108516; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC008650; AAH08650.1; -; mRNA.
DR EMBL; BC014327; AAH14327.1; -; mRNA.
DR EMBL; BC016367; AAH16367.1; -; mRNA.
DR EMBL; BC021673; AAH21673.1; -; mRNA.
DR EMBL; BC036001; AAH36001.1; -; mRNA.
DR RefSeq; NP_057329.2; NM_016245.3.
DR UniGene; Hs.594923; -.
DR PDB; 1YB1; X-ray; 1.95 A; A/B=28-275.
DR PDBsum; 1YB1; -.
DR ProteinModelPortal; Q8NBQ5; -.
DR SMR; Q8NBQ5; 30-273.
DR PhosphoSite; Q8NBQ5; -.
DR DMDM; 296439374; -.
DR PaxDb; Q8NBQ5; -.
DR PRIDE; Q8NBQ5; -.
DR DNASU; 51170; -.
DR Ensembl; ENST00000358290; ENSP00000351035; ENSG00000198189.
DR GeneID; 51170; -.
DR KEGG; hsa:51170; -.
DR UCSC; uc003hqp.2; human.
DR CTD; 51170; -.
DR GeneCards; GC04M088257; -.
DR H-InvDB; HIX0004358; -.
DR HGNC; HGNC:22960; HSD17B11.
DR HPA; HPA021608; -.
DR MIM; 612831; gene.
DR neXtProt; NX_Q8NBQ5; -.
DR PharmGKB; PA162391655; -.
DR eggNOG; COG1028; -.
DR HOVERGEN; HBG051352; -.
DR InParanoid; Q8NBQ5; -.
DR OMA; IGYKTKG; -.
DR OrthoDB; EOG7Z3F50; -.
DR PhylomeDB; Q8NBQ5; -.
DR ChiTaRS; HSD17B11; human.
DR EvolutionaryTrace; Q8NBQ5; -.
DR GeneWiki; HSD17B11; -.
DR GenomeRNAi; 51170; -.
DR NextBio; 54111; -.
DR PRO; PR:Q8NBQ5; -.
DR ArrayExpress; Q8NBQ5; -.
DR Bgee; Q8NBQ5; -.
DR CleanEx; HS_HSD17B11; -.
DR Genevestigator; Q8NBQ5; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid particle; IDA:UniProtKB.
DR GO; GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016229; F:steroid dehydrogenase activity; IDA:HGNC.
DR GO; GO:0006710; P:androgen catabolic process; IDA:HGNC.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.720; -; 1.
DR InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR InterPro; IPR002347; Glc/ribitol_DH.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR Pfam; PF00106; adh_short; 1.
DR PIRSF; PIRSF000126; 11-beta-HSD1; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR PROSITE; PS00061; ADH_SHORT; FALSE_NEG.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Lipid biosynthesis; Lipid metabolism;
KW NADP; Oxidoreductase; Reference proteome; Secreted; Signal;
KW Steroid biosynthesis.
FT SIGNAL 1 19 Potential.
FT CHAIN 20 300 Estradiol 17-beta-dehydrogenase 11.
FT /FTId=PRO_0000031970.
FT NP_BIND 40 64 NADP (By similarity).
FT ACT_SITE 185 185 Proton acceptor (By similarity).
FT BINDING 172 172 Substrate (By similarity).
FT CONFLICT 23 23 V -> E (in Ref. 6; AAH08650).
FT CONFLICT 38 38 I -> T (in Ref. 4; BAC11560).
FT CONFLICT 106 106 K -> N (in Ref. 6; AAH08650).
FT CONFLICT 107 107 V -> C (in Ref. 6; AAH08650).
FT CONFLICT 228 228 N -> K (in Ref. 6; AAH08650).
FT CONFLICT 229 229 P -> M (in Ref. 6; AAH08650).
FT CONFLICT 263 263 I -> N (in Ref. 6; AAH08650).
FT CONFLICT 264 264 A -> C (in Ref. 6; AAH08650).
FT CONFLICT 265 265 F -> I (in Ref. 6; AAH08650).
FT CONFLICT 283 283 Q -> R (in Ref. 1; AAF06939, 2; AAM44459,
FT 3; AAQ88917, 4; BAC11560 and 6; AAH08650/
FT AAH14327/AAH16367/AAH21673/AAH36001).
FT STRAND 38 42
FT TURN 43 45
FT HELIX 47 58
FT STRAND 62 68
FT HELIX 70 82
FT STRAND 87 91
FT HELIX 97 110
FT STRAND 115 119
FT HELIX 130 132
FT HELIX 133 144
FT HELIX 146 161
FT STRAND 165 170
FT HELIX 179 205
FT STRAND 211 218
FT HELIX 220 223
FT HELIX 230 233
FT HELIX 239 251
FT STRAND 255 259
FT HELIX 265 270
SQ SEQUENCE 300 AA; 32936 MW; 51DE633A205EBE86 CRC64;
MKFLLDILLL LPLLIVCSLE SFVKLFIPKR RKSVTGEIVL ITGAGHGIGR LTAYEFAKLK
SKLVLWDINK HGLEETAAKC KGLGAKVHTF VVDCSNREDI YSSAKKVKAE IGDVSILVNN
AGVVYTSDLF ATQDPQIEKT FEVNVLAHFW TTKAFLPAMT KNNHGHIVTV ASAAGHVSVP
FLLAYCSSKF AAVGFHKTLT DELAALQITG VKTTCLCPNF VNTGFIKNPS TSLGPTLEPE
EVVNRLMHGI LTEQKMIFIP SSIAFLTTLE RILPERFLAV LKQKISVKFD AVIGYKMKAQ
//
ID DHB11_HUMAN Reviewed; 300 AA.
AC Q8NBQ5; Q96HF6; Q9UKU4;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
read moreDT 18-MAY-2010, sequence version 3.
DT 22-JAN-2014, entry version 104.
DE RecName: Full=Estradiol 17-beta-dehydrogenase 11;
DE EC=1.1.1.62;
DE AltName: Full=17-beta-hydroxysteroid dehydrogenase 11;
DE Short=17-beta-HSD 11;
DE Short=17bHSD11;
DE Short=17betaHSD11;
DE AltName: Full=17-beta-hydroxysteroid dehydrogenase XI;
DE Short=17-beta-HSD XI;
DE Short=17betaHSDXI;
DE AltName: Full=Cutaneous T-cell lymphoma-associated antigen HD-CL-03;
DE Short=CTCL-associated antigen HD-CL-03;
DE AltName: Full=Dehydrogenase/reductase SDR family member 8;
DE AltName: Full=Retinal short-chain dehydrogenase/reductase 2;
DE Short=retSDR2;
DE Flags: Precursor;
GN Name=HSD17B11; Synonyms=DHRS8, PAN1B;
GN ORFNames=PSEC0029, UNQ207/PRO233;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Retina;
RX PubMed=10800688; DOI=10.1016/S0076-6879(00)16736-9;
RA Haeseleer F., Palczewski K.;
RT "Short-chain dehydrogenases/reductases in retina.";
RL Methods Enzymol. 316:372-383(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lymphoma;
RX PubMed=14996095; DOI=10.1111/j.1365-2133.2004.05651.x;
RA Hartmann T.B., Thiel D., Dummer R., Schadendorf D., Eichmueller S.;
RT "SEREX identification of new tumour-associated antigens in cutaneous
RT T-cell lymphoma.";
RL Br. J. Dermatol. 150:252-258(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S.,
RA Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J.,
RA Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J.,
RA Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A.,
RA Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H.,
RA Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D.,
RA Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale
RT effort to identify novel human secreted and transmembrane proteins: a
RT bioinformatics assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Teratocarcinoma;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-
RT length human cDNAs encoding secretion or membrane proteins from oligo-
RT capped cDNA libraries.";
RL DNA Res. 12:117-126(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA Waterston R.H., Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2
RT and 4.";
RL Nature 434:724-731(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Bone marrow, Colon, Kidney, Liver, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=9888557;
RA Li K.X.Z., Smith R.E., Krozowski Z.S.;
RT "Cloning and expression of a novel tissue specific 17beta-
RT hydroxysteroid dehydrogenase.";
RL Endocr. Res. 24:663-667(1998).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=11165019; DOI=10.1016/S0303-7207(00)00417-2;
RA Brereton P., Suzuki T., Sasano H., Li K., Duarte C., Obeyesekere V.,
RA Haeseleer F., Palczewski K., Smith I., Komesaroff P., Krozowski Z.;
RT "Pan1b (17betaHSD11)-enzymatic activity and distribution in the
RT lung.";
RL Mol. Cell. Endocrinol. 171:111-117(2001).
RN [9]
RP ENZYME ACTIVITY IN VITRO, AND TISSUE SPECIFICITY.
RX PubMed=12697717; DOI=10.1210/en.2002-221030;
RA Chai Z., Brereton P., Suzuki T., Sasano H., Obeyesekere V., Escher G.,
RA Saffery R., Fuller P., Enriquez C., Krozowski Z.;
RT "17 beta-hydroxysteroid dehydrogenase type XI localizes to human
RT steroidogenic cells.";
RL Endocrinology 144:2084-2091(2003).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 28-275.
RA Lukacik P., Bunkoczi G., Kavanagh K., Ng S., Von delft F., Bray J.,
RA Edwards A., Arrowsmith C., Sundstrom M., Oppermann U.;
RT "Crystal structure of human 17-beta-hydroxysteroid dehydrogenase type
RT XI.";
RL Submitted (DEC-2004) to the PDB data bank.
CC -!- FUNCTION: Can convert androstan-3-alpha,17-beta-diol (3-alpha-
CC diol) to androsterone in vitro, suggesting that it may participate
CC in androgen metabolism during steroidogenesis. May act by
CC metabolizing compounds that stimulate steroid synthesis and/or by
CC generating metabolites that inhibit it. Has no activity toward
CC DHEA (dehydroepiandrosterone), or A-dione (4-androste-3,17-dione),
CC and only a slight activity toward testosterone to A-dione. Tumor-
CC associated antigen in cutaneous T-cell lymphoma.
CC -!- CATALYTIC ACTIVITY: 17-beta-estradiol + NAD(P)(+) = estrone +
CC NAD(P)H.
CC -!- SUBCELLULAR LOCATION: Secreted (Potential).
CC -!- TISSUE SPECIFICITY: Present at high level in steroidogenic cells
CC such as syncytiotrophoblasts, sebaceous gland, Leydig cells, and
CC granulosa cells of the dominant follicle and corpus luteum. In
CC lung, it is detected in the ciliated epithelium and in acini of
CC adult trachea, in bronchioles, but not in alveoli. In the eye, it
CC is detected in the nonpigmented epithelium of the ciliary body
CC and, at lower level, in the inner nuclear layer of the retina (at
CC protein level). Widely expressed. Highly expressed in retina,
CC pancreas, kidney, liver, lung, adrenal, small intestine, ovary and
CC heart.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC (SDR) family. 17-beta-HSD 3 subfamily.
CC -----------------------------------------------------------------------
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DR EMBL; AF126780; AAF06939.1; -; mRNA.
DR EMBL; AF273056; AAM44459.1; -; mRNA.
DR EMBL; AY358553; AAQ88917.1; -; mRNA.
DR EMBL; AK075348; BAC11560.1; -; mRNA.
DR EMBL; AC108516; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC008650; AAH08650.1; -; mRNA.
DR EMBL; BC014327; AAH14327.1; -; mRNA.
DR EMBL; BC016367; AAH16367.1; -; mRNA.
DR EMBL; BC021673; AAH21673.1; -; mRNA.
DR EMBL; BC036001; AAH36001.1; -; mRNA.
DR RefSeq; NP_057329.2; NM_016245.3.
DR UniGene; Hs.594923; -.
DR PDB; 1YB1; X-ray; 1.95 A; A/B=28-275.
DR PDBsum; 1YB1; -.
DR ProteinModelPortal; Q8NBQ5; -.
DR SMR; Q8NBQ5; 30-273.
DR PhosphoSite; Q8NBQ5; -.
DR DMDM; 296439374; -.
DR PaxDb; Q8NBQ5; -.
DR PRIDE; Q8NBQ5; -.
DR DNASU; 51170; -.
DR Ensembl; ENST00000358290; ENSP00000351035; ENSG00000198189.
DR GeneID; 51170; -.
DR KEGG; hsa:51170; -.
DR UCSC; uc003hqp.2; human.
DR CTD; 51170; -.
DR GeneCards; GC04M088257; -.
DR H-InvDB; HIX0004358; -.
DR HGNC; HGNC:22960; HSD17B11.
DR HPA; HPA021608; -.
DR MIM; 612831; gene.
DR neXtProt; NX_Q8NBQ5; -.
DR PharmGKB; PA162391655; -.
DR eggNOG; COG1028; -.
DR HOVERGEN; HBG051352; -.
DR InParanoid; Q8NBQ5; -.
DR OMA; IGYKTKG; -.
DR OrthoDB; EOG7Z3F50; -.
DR PhylomeDB; Q8NBQ5; -.
DR ChiTaRS; HSD17B11; human.
DR EvolutionaryTrace; Q8NBQ5; -.
DR GeneWiki; HSD17B11; -.
DR GenomeRNAi; 51170; -.
DR NextBio; 54111; -.
DR PRO; PR:Q8NBQ5; -.
DR ArrayExpress; Q8NBQ5; -.
DR Bgee; Q8NBQ5; -.
DR CleanEx; HS_HSD17B11; -.
DR Genevestigator; Q8NBQ5; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid particle; IDA:UniProtKB.
DR GO; GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016229; F:steroid dehydrogenase activity; IDA:HGNC.
DR GO; GO:0006710; P:androgen catabolic process; IDA:HGNC.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.720; -; 1.
DR InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR InterPro; IPR002347; Glc/ribitol_DH.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR Pfam; PF00106; adh_short; 1.
DR PIRSF; PIRSF000126; 11-beta-HSD1; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR PROSITE; PS00061; ADH_SHORT; FALSE_NEG.
PE 1: Evidence at protein level;
KW 3D-structure; Complete proteome; Lipid biosynthesis; Lipid metabolism;
KW NADP; Oxidoreductase; Reference proteome; Secreted; Signal;
KW Steroid biosynthesis.
FT SIGNAL 1 19 Potential.
FT CHAIN 20 300 Estradiol 17-beta-dehydrogenase 11.
FT /FTId=PRO_0000031970.
FT NP_BIND 40 64 NADP (By similarity).
FT ACT_SITE 185 185 Proton acceptor (By similarity).
FT BINDING 172 172 Substrate (By similarity).
FT CONFLICT 23 23 V -> E (in Ref. 6; AAH08650).
FT CONFLICT 38 38 I -> T (in Ref. 4; BAC11560).
FT CONFLICT 106 106 K -> N (in Ref. 6; AAH08650).
FT CONFLICT 107 107 V -> C (in Ref. 6; AAH08650).
FT CONFLICT 228 228 N -> K (in Ref. 6; AAH08650).
FT CONFLICT 229 229 P -> M (in Ref. 6; AAH08650).
FT CONFLICT 263 263 I -> N (in Ref. 6; AAH08650).
FT CONFLICT 264 264 A -> C (in Ref. 6; AAH08650).
FT CONFLICT 265 265 F -> I (in Ref. 6; AAH08650).
FT CONFLICT 283 283 Q -> R (in Ref. 1; AAF06939, 2; AAM44459,
FT 3; AAQ88917, 4; BAC11560 and 6; AAH08650/
FT AAH14327/AAH16367/AAH21673/AAH36001).
FT STRAND 38 42
FT TURN 43 45
FT HELIX 47 58
FT STRAND 62 68
FT HELIX 70 82
FT STRAND 87 91
FT HELIX 97 110
FT STRAND 115 119
FT HELIX 130 132
FT HELIX 133 144
FT HELIX 146 161
FT STRAND 165 170
FT HELIX 179 205
FT STRAND 211 218
FT HELIX 220 223
FT HELIX 230 233
FT HELIX 239 251
FT STRAND 255 259
FT HELIX 265 270
SQ SEQUENCE 300 AA; 32936 MW; 51DE633A205EBE86 CRC64;
MKFLLDILLL LPLLIVCSLE SFVKLFIPKR RKSVTGEIVL ITGAGHGIGR LTAYEFAKLK
SKLVLWDINK HGLEETAAKC KGLGAKVHTF VVDCSNREDI YSSAKKVKAE IGDVSILVNN
AGVVYTSDLF ATQDPQIEKT FEVNVLAHFW TTKAFLPAMT KNNHGHIVTV ASAAGHVSVP
FLLAYCSSKF AAVGFHKTLT DELAALQITG VKTTCLCPNF VNTGFIKNPS TSLGPTLEPE
EVVNRLMHGI LTEQKMIFIP SSIAFLTTLE RILPERFLAV LKQKISVKFD AVIGYKMKAQ
//
MIM
612831
*RECORD*
*FIELD* NO
612831
*FIELD* TI
*612831 17-@BETA-HYDROXYSTEROID DEHYDROGENASE XI; HSD17B11
;;17-@BETA-HSDXI;;
RETSDR2;;
read morePAN1B
*FIELD* TX
DESCRIPTION
Short-chain alcohol dehydrogenases, such as HSD17B11, metabolize
secondary alcohols and ketones (Brereton et al., 2001).
CLONING
By searching EST databases for sequences similar to 11-beta-HSD1
(HSD11B1; 600713), followed by screening a lung cDNA library, Li et al.
(1998) cloned HSD17B11, which they called PAN1B. Northern blot analysis
detected high expression of a 1.9-kb transcript in liver, lung, small
intestine, adrenal cortex, medulla, and adrenal carcinoma. Lower
expression was detected in kidney, heart, placenta, stomach, thymus,
testis, thyroid, and pancreas.
By searching EST databases for short-chain dehydrogenases/reductases
(SDRs), followed by 3-prime and 5-prime RACE and nested PCR of a human
retina cDNA library, Haeseleer and Palczewski (2000) cloned HSD17B11,
which they called RETSDR2. The deduced 300-amino acid protein has a
calculated molecular mass of 33.0 kD. It has all the characteristics of
SDRs, including a cofactor-binding Rossman-fold domain and highly
conserved motifs in the nucleotide-binding site and active site.
Northern blot analysis detected HSD17B11 expression in all tissues
examined.
By immunohistochemical analysis, Brereton et al. (2001) detected PAN1B
in ciliated epithelium and acini of adult human trachea and in
bronchioles, but not in alveoli. Similar staining was detected in human
fetus, with stronger staining in the second half of pregnancy. In the
eye, PAN1B was detected in nonpigmented epithelium of the ciliary body,
with lower expression in the inner nuclear layer of the retina.
Expression was also detected in normal adrenal cortex, granulosa cells,
paraluteal cells, Leydig cells, and syncytiotrophoblasts.
Chai et al. (2003) cloned 17-beta-HSDXI from a pregnant human uterus
cDNA library. The deduced 300-amino acid protein has a potential
N-glycosylation site at residue 228. The 5-prime UTR of the cDNA has a
polymorphic adenosine repeat. Northern blot analysis detected high
17-beta-HSDXI expression in pancreas, kidney, liver, lung, adrenal
gland, ovary, and heart. Immunohistochemical analysis showed strong
expression in steroidogenic cells, such as syncytiotrophoblasts,
sebaceous gland, Leydig cells, and granulosa cells of the dominant
follicle and corpus luteum. Expression in fetal adrenal gland increased
after birth.
GENE FUNCTION
By assaying transfected CHOP Chinese hamster ovary cells, Li et al.
(1998) showed that PAN1B converted estradiol to estrone. It did not use
other steroid substrates tested, including glucocorticoids.
Using several 17-keto- and 17-beta-hydroxysteroids as substrates,
Brereton et al. (2001) showed that CHOP cells expressing PAN1B converted
the androgen metabolite 3-alpha-diol
(5-alpha-androstane-3-alpha,17-beta-diol) to androsterone in a
nonsaturable reaction. Several retinoids inhibited 3-alpha-diol
metabolism more than 90%, but PAN1B did not metabolize these retinoids.
Chai et al. (2003) found that cAMP downregulated 17-beta-Hsdxi enzymatic
activity in a mouse adrenocortical cell line and reduced 17-beta-Hsdxi
message to undetectable levels. All-trans-retinoic acid (RA) did not
affect 17-beta-Hsdxi expression or activity, but addition of RA together
with cAMP significantly decreased activity over cAMP alone.
GENE STRUCTURE
Chai et al. (2003) found that the 5-prime flanking region of the
HSD17B11 gene contains a CpG island. They also identified a potential
nuclear receptor steroidogenic factor-1 (SF1, or NR5A1; 184757)
half-site in the HSD17B11 promoter and a cluster of 3 other potential
SF1 half-sites in the distal part of intron 1.
MAPPING
By radiation hybrid and genomic sequence analyses, Chai et al. (2003)
mapped the HSD17B11 gene to chromosome 4q21.
MOLECULAR GENETICS
By EST database analysis, Chai et al. (2003) identified HSD17B11 cDNAs
containing 10 to 20 adenosine repeats in the 5-prime UTR. However, 15
repeats were found in all 52 normal individuals examined, suggesting
that other repeat lengths are rare. An HSD17B11 construct with 20
adenosines produced more enzyme activity than constructs containing 15
or less adenosines.
*FIELD* RF
1. Brereton, P.; Suzuki, T.; Sasano, H.; Li, K.; Duarte, C.; Obeyesekere,
V.; Haeseleer, F.; Palczewski, K.; Smith, I.; Komesaroff, P.; Krozowski,
Z.: Pan1b (17-beta-HSD11)-enzymatic activity and distribution in
the lung. Molec. Cell. Endocr. 171: 111-117, 2001.
2. Chai, Z.; Brereton, P.; Suzuki, T.; Sasano, H.; Obeyesekere, V.;
Escher, G.; Saffery, R.; Fuller, P.; Enriquez, C.; Krozowski, Z.:
17-beta-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic
cells. Endocrinology 144: 2084-2091, 2003.
3. Haeseleer, F.; Palczewski, K.: Short-chain dehydrogenases/reductases
in retina. Methods Enzymol. 316: 372-383, 2000.
4. Li, K. X. Z.; Smith, R. E.; Krozowski, Z. S.: Cloning and expression
of a novel tissue specific 17-beta-hydroxysteroid dehydrogenase. Endocr.
Res. 24: 663-667, 1998.
*FIELD* CD
Patricia A. Hartz: 5/28/2009
*FIELD* ED
mgross: 05/28/2009
*RECORD*
*FIELD* NO
612831
*FIELD* TI
*612831 17-@BETA-HYDROXYSTEROID DEHYDROGENASE XI; HSD17B11
;;17-@BETA-HSDXI;;
RETSDR2;;
read morePAN1B
*FIELD* TX
DESCRIPTION
Short-chain alcohol dehydrogenases, such as HSD17B11, metabolize
secondary alcohols and ketones (Brereton et al., 2001).
CLONING
By searching EST databases for sequences similar to 11-beta-HSD1
(HSD11B1; 600713), followed by screening a lung cDNA library, Li et al.
(1998) cloned HSD17B11, which they called PAN1B. Northern blot analysis
detected high expression of a 1.9-kb transcript in liver, lung, small
intestine, adrenal cortex, medulla, and adrenal carcinoma. Lower
expression was detected in kidney, heart, placenta, stomach, thymus,
testis, thyroid, and pancreas.
By searching EST databases for short-chain dehydrogenases/reductases
(SDRs), followed by 3-prime and 5-prime RACE and nested PCR of a human
retina cDNA library, Haeseleer and Palczewski (2000) cloned HSD17B11,
which they called RETSDR2. The deduced 300-amino acid protein has a
calculated molecular mass of 33.0 kD. It has all the characteristics of
SDRs, including a cofactor-binding Rossman-fold domain and highly
conserved motifs in the nucleotide-binding site and active site.
Northern blot analysis detected HSD17B11 expression in all tissues
examined.
By immunohistochemical analysis, Brereton et al. (2001) detected PAN1B
in ciliated epithelium and acini of adult human trachea and in
bronchioles, but not in alveoli. Similar staining was detected in human
fetus, with stronger staining in the second half of pregnancy. In the
eye, PAN1B was detected in nonpigmented epithelium of the ciliary body,
with lower expression in the inner nuclear layer of the retina.
Expression was also detected in normal adrenal cortex, granulosa cells,
paraluteal cells, Leydig cells, and syncytiotrophoblasts.
Chai et al. (2003) cloned 17-beta-HSDXI from a pregnant human uterus
cDNA library. The deduced 300-amino acid protein has a potential
N-glycosylation site at residue 228. The 5-prime UTR of the cDNA has a
polymorphic adenosine repeat. Northern blot analysis detected high
17-beta-HSDXI expression in pancreas, kidney, liver, lung, adrenal
gland, ovary, and heart. Immunohistochemical analysis showed strong
expression in steroidogenic cells, such as syncytiotrophoblasts,
sebaceous gland, Leydig cells, and granulosa cells of the dominant
follicle and corpus luteum. Expression in fetal adrenal gland increased
after birth.
GENE FUNCTION
By assaying transfected CHOP Chinese hamster ovary cells, Li et al.
(1998) showed that PAN1B converted estradiol to estrone. It did not use
other steroid substrates tested, including glucocorticoids.
Using several 17-keto- and 17-beta-hydroxysteroids as substrates,
Brereton et al. (2001) showed that CHOP cells expressing PAN1B converted
the androgen metabolite 3-alpha-diol
(5-alpha-androstane-3-alpha,17-beta-diol) to androsterone in a
nonsaturable reaction. Several retinoids inhibited 3-alpha-diol
metabolism more than 90%, but PAN1B did not metabolize these retinoids.
Chai et al. (2003) found that cAMP downregulated 17-beta-Hsdxi enzymatic
activity in a mouse adrenocortical cell line and reduced 17-beta-Hsdxi
message to undetectable levels. All-trans-retinoic acid (RA) did not
affect 17-beta-Hsdxi expression or activity, but addition of RA together
with cAMP significantly decreased activity over cAMP alone.
GENE STRUCTURE
Chai et al. (2003) found that the 5-prime flanking region of the
HSD17B11 gene contains a CpG island. They also identified a potential
nuclear receptor steroidogenic factor-1 (SF1, or NR5A1; 184757)
half-site in the HSD17B11 promoter and a cluster of 3 other potential
SF1 half-sites in the distal part of intron 1.
MAPPING
By radiation hybrid and genomic sequence analyses, Chai et al. (2003)
mapped the HSD17B11 gene to chromosome 4q21.
MOLECULAR GENETICS
By EST database analysis, Chai et al. (2003) identified HSD17B11 cDNAs
containing 10 to 20 adenosine repeats in the 5-prime UTR. However, 15
repeats were found in all 52 normal individuals examined, suggesting
that other repeat lengths are rare. An HSD17B11 construct with 20
adenosines produced more enzyme activity than constructs containing 15
or less adenosines.
*FIELD* RF
1. Brereton, P.; Suzuki, T.; Sasano, H.; Li, K.; Duarte, C.; Obeyesekere,
V.; Haeseleer, F.; Palczewski, K.; Smith, I.; Komesaroff, P.; Krozowski,
Z.: Pan1b (17-beta-HSD11)-enzymatic activity and distribution in
the lung. Molec. Cell. Endocr. 171: 111-117, 2001.
2. Chai, Z.; Brereton, P.; Suzuki, T.; Sasano, H.; Obeyesekere, V.;
Escher, G.; Saffery, R.; Fuller, P.; Enriquez, C.; Krozowski, Z.:
17-beta-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic
cells. Endocrinology 144: 2084-2091, 2003.
3. Haeseleer, F.; Palczewski, K.: Short-chain dehydrogenases/reductases
in retina. Methods Enzymol. 316: 372-383, 2000.
4. Li, K. X. Z.; Smith, R. E.; Krozowski, Z. S.: Cloning and expression
of a novel tissue specific 17-beta-hydroxysteroid dehydrogenase. Endocr.
Res. 24: 663-667, 1998.
*FIELD* CD
Patricia A. Hartz: 5/28/2009
*FIELD* ED
mgross: 05/28/2009