Full text data of DPYSL2
DPYSL2
(CRMP2, ULIP2)
[Confidence: low (only semi-automatic identification from reviews)]
Dihydropyrimidinase-related protein 2; DRP-2 (Collapsin response mediator protein 2; CRMP-2; N2A3; Unc-33-like phosphoprotein 2; ULIP-2)
Dihydropyrimidinase-related protein 2; DRP-2 (Collapsin response mediator protein 2; CRMP-2; N2A3; Unc-33-like phosphoprotein 2; ULIP-2)
UniProt
Q16555
ID DPYL2_HUMAN Reviewed; 572 AA.
AC Q16555; A8K5H2; B4DR31; D3DSS7; O00424;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1996, sequence version 1.
DT 22-JAN-2014, entry version 140.
DE RecName: Full=Dihydropyrimidinase-related protein 2;
DE Short=DRP-2;
DE AltName: Full=Collapsin response mediator protein 2;
DE Short=CRMP-2;
DE AltName: Full=N2A3;
DE AltName: Full=Unc-33-like phosphoprotein 2;
DE Short=ULIP-2;
GN Name=DPYSL2; Synonyms=CRMP2, ULIP2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=7637782; DOI=10.1038/376509a0;
RA Goshima Y., Nakamura F., Strittmatter P., Strittmatter S.M.;
RT "Collapsin-induced growth cone collapse mediated by an intracellular
RT protein related to UNC-33.";
RL Nature 376:509-514(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=8973361; DOI=10.1016/S0378-1119(96)00445-3;
RA Hamajima N., Matsuda K., Sakata S., Tamaki N., Sasaki M., Nonaka M.;
RT "A novel gene family defined by human dihydropyrimidinase and three
RT related proteins with differential tissue distribution.";
RL Gene 180:157-163(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Fetal liver;
RA Zhou J., Chen Y., Gu J.R.;
RT "A cDNA clone highly expressed in human brain and deleted in liver
RT cancer.";
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10574455; DOI=10.1093/dnares/6.5.291;
RA Kitamura K., Takayama M., Hamajima N., Nakanishi M., Sasaki M.,
RA Endo Y., Takemoto T., Kimura H., Iwaki M., Nonaka M.;
RT "Characterization of the human dihydropyrimidinase-related protein 2
RT (DRP-2) gene.";
RL DNA Res. 6:291-297(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S.,
RA Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A.,
RA Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Allen N.R., Anderson S., Asakawa T., Blechschmidt K., Bloom T.,
RA Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K.,
RA DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G.,
RA Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B.,
RA Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C.,
RA O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K.,
RA Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R.,
RA Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K.,
RA Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q.,
RA Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N.,
RA Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Eye, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP PROTEIN SEQUENCE OF 44-56; 64-75; 147-157; 174-211; 239-254; 375-390;
RP 401-418; 424-467; 497-511 AND 533-552, AND MASS SPECTROMETRY.
RC TISSUE=Brain, Cajal-Retzius cell, and Fetal brain cortex;
RA Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.;
RL Submitted (DEC-2008) to UniProtKB.
RN [10]
RP PHOSPHORYLATION AT SER-518; SER-522 AND THR-509, AND MUTAGENESIS OF
RP SER-507; THR-509; THR-512; THR-514; SER-517; SER-518; THR-521 AND
RP SER-522.
RX PubMed=10757975; DOI=10.1021/bi992323h;
RA Gu Y., Hamajima N., Ihara Y.;
RT "Neurofibrillary tangle-associated collapsin response mediator
RT protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518, and
RT Ser-522.";
RL Biochemistry 39:4267-4275(2000).
RN [11]
RP FUNCTION.
RX PubMed=11477421; DOI=10.1038/90476;
RA Inagaki N., Chihara K., Arimura N., Menager C., Kawano Y., Matsuo N.,
RA Nishimura T., Amano M., Kaibuchi K.;
RT "CRMP-2 induces axons in cultured hippocampal neurons.";
RL Nat. Neurosci. 4:781-782(2001).
RN [12]
RP FUNCTION, AND PHOSPHORYLATION AT SER-522.
RX PubMed=15466863; DOI=10.1074/jbc.C400412200;
RA Cole A.R., Knebel A., Morrice N.A., Robertson L.A., Irving A.J.,
RA Connolly C.N., Sutherland C.;
RT "GSK-3 phosphorylation of the Alzheimer epitope within collapsin
RT response mediator proteins regulates axon elongation in primary
RT neurons.";
RL J. Biol. Chem. 279:50176-50180(2004).
RN [13]
RP INTERACTION WITH CYFIP1, AND MUTAGENESIS OF ASP-71.
RX PubMed=16260607; DOI=10.1128/MCB.25.22.9920-9935.2005;
RA Kawano Y., Yoshimura T., Tsuboi D., Kawabata S., Kaneko-Kawano T.,
RA Shirataki H., Takenawa T., Kaibuchi K.;
RT "CRMP-2 is involved in kinesin-1-dependent transport of the Sra-
RT 1/WAVE1 complex and axon formation.";
RL Mol. Cell. Biol. 25:9920-9935(2005).
RN [14]
RP INTERACTION WITH PLEXNA1, AND SUBUNIT.
RX PubMed=14685275; DOI=10.1038/sj.emboj.7600021;
RA Deo R.C., Schmidt E.F., Elhabazi A., Togashi H., Burley S.K.,
RA Strittmatter S.M.;
RT "Structural bases for CRMP function in plexin-dependent semaphorin3A
RT signaling.";
RL EMBO J. 23:9-22(2004).
RN [15]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-509 AND SER-522, AND
RP MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=16964243; DOI=10.1038/nbt1240;
RA Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT "A probability-based approach for high-throughput protein
RT phosphorylation analysis and site localization.";
RL Nat. Biotechnol. 24:1285-1292(2006).
RN [16]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-509, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [17]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [18]
RP PHOSPHORYLATION AT TYR-32 BY FYN.
RX PubMed=19652227; DOI=10.1074/jbc.M109.000240;
RA Uchida Y., Ohshima T., Yamashita N., Ogawara M., Sasaki Y.,
RA Nakamura F., Goshima Y.;
RT "Semaphorin3A signaling mediated by Fyn-dependent tyrosine
RT phosphorylation of collapsin response mediator protein 2 at tyrosine
RT 32.";
RL J. Biol. Chem. 284:27393-27401(2009).
RN [19]
RP INTERACTION WITH CLN6.
RX PubMed=19235893; DOI=10.1002/jnr.22032;
RA Benedict J.W., Getty A.L., Wishart T.M., Gillingwater T.H.,
RA Pearce D.A.;
RT "Protein product of CLN6 gene responsible for variant late-onset
RT infantile neuronal ceroid lipofuscinosis interacts with CRMP-2.";
RL J. Neurosci. Res. 87:2157-2166(2009).
RN [20]
RP FUNCTION IN ENDOCYTOSIS, INTERACTION WITH MICALL1, AND SUBCELLULAR
RP LOCATION.
RX PubMed=20801876; DOI=10.1074/jbc.C110.166066;
RA Rahajeng J., Giridharan S.S., Naslavsky N., Caplan S.;
RT "Collapsin response mediator protein-2 (Crmp2) regulates trafficking
RT by linking endocytic regulatory proteins to dynein motors.";
RL J. Biol. Chem. 285:31918-31922(2010).
RN [21]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [22]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [23]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-509; THR-514; SER-517;
RP SER-518 AND SER-522, AND MASS SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [24]
RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 12-490, AND SUBUNIT.
RX PubMed=17250651; DOI=10.1111/j.1471-4159.2006.04401.x;
RA Stenmark P., Ogg D., Flodin S., Flores A., Kotenyova T., Nyman T.,
RA Nordlund P., Kursula P.;
RT "The structure of human collapsin response mediator protein 2, a
RT regulator of axonal growth.";
RL J. Neurochem. 101:906-917(2007).
RN [25]
RP VARIANT [LARGE SCALE ANALYSIS] CYS-481.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S.,
RA Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J.,
RA Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C.,
RA Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N.,
RA Vogelstein B., Kinzler K.W., Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal
RT cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Plays a role in neuronal development and polarity, as
CC well as in axon growth and guidance, neuronal growth cone collapse
CC and cell migration. Necessary for signaling by class 3 semaphorins
CC and subsequent remodeling of the cytoskeleton. May play a role in
CC endocytosis.
CC -!- SUBUNIT: Homotetramer, and heterotetramer with CRMP1, DPYSL3,
CC DPYSL4 or DPYSL5. Interacts through its C-terminus with the C-
CC terminus of CYFIP1/SRA1. Interacts with HTR4. Interacts with CLN6.
CC Interacts with MICALL1.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasm, cytoskeleton.
CC Membrane. Note=Tightly but non-covalently associated with
CC membranes.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q16555-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q16555-2; Sequence=VSP_044941;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- PTM: 3F4, a monoclonal antibody which strongly stains
CC neurofibrillary tangles in Alzheimer disease brains, specifically
CC labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
CC -!- PTM: Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding
CC leading to destabilization of microtubule assembly in axons and
CC neurodegeneration (By similarity). Phosphorylation by DYRK2 at
CC Ser-522 is required for subsequent phosphorylation by GSK3B.
CC -!- SIMILARITY: Belongs to the DHOase family.
CC Hydantoinase/dihydropyrimidinase subfamily.
CC -!- CAUTION: Lacks most of the conserved residues that are essential
CC for binding the metal cofactor and hence for dihydropyrimidinase
CC activity. Its enzyme activity is therefore unsure.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; U17279; AAA93202.1; -; mRNA.
DR EMBL; D78013; BAA11191.1; -; mRNA.
DR EMBL; U97105; AAC05793.1; -; mRNA.
DR EMBL; AB020777; BAA86991.1; -; Genomic_DNA.
DR EMBL; AK291287; BAF83976.1; -; mRNA.
DR EMBL; AK299077; BAG61143.1; -; mRNA.
DR EMBL; AC015564; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC015743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471080; EAW63573.1; -; Genomic_DNA.
DR EMBL; CH471080; EAW63574.1; -; Genomic_DNA.
DR EMBL; BC056408; AAH56408.1; -; mRNA.
DR EMBL; BC067109; AAH67109.1; -; mRNA.
DR PIR; JC5317; JC5317.
DR RefSeq; NP_001184222.1; NM_001197293.2.
DR RefSeq; NP_001231533.1; NM_001244604.1.
DR RefSeq; NP_001377.1; NM_001386.5.
DR UniGene; Hs.593187; -.
DR PDB; 2GSE; X-ray; 2.40 A; A/B/C/D=13-490.
DR PDB; 2VM8; X-ray; 1.90 A; A/B/C/D=13-490.
DR PDBsum; 2GSE; -.
DR PDBsum; 2VM8; -.
DR ProteinModelPortal; Q16555; -.
DR SMR; Q16555; 14-490.
DR IntAct; Q16555; 13.
DR MINT; MINT-3032752; -.
DR STRING; 9606.ENSP00000309539; -.
DR MEROPS; M38.975; -.
DR PhosphoSite; Q16555; -.
DR DMDM; 3122051; -.
DR REPRODUCTION-2DPAGE; IPI00257508; -.
DR REPRODUCTION-2DPAGE; Q16555; -.
DR UCD-2DPAGE; Q16555; -.
DR PaxDb; Q16555; -.
DR PeptideAtlas; Q16555; -.
DR PRIDE; Q16555; -.
DR Ensembl; ENST00000311151; ENSP00000309539; ENSG00000092964.
DR Ensembl; ENST00000521913; ENSP00000427985; ENSG00000092964.
DR Ensembl; ENST00000523027; ENSP00000431117; ENSG00000092964.
DR GeneID; 1808; -.
DR KEGG; hsa:1808; -.
DR UCSC; uc003xfb.2; human.
DR CTD; 1808; -.
DR GeneCards; GC08P026371; -.
DR HGNC; HGNC:3014; DPYSL2.
DR HPA; CAB018719; -.
DR HPA; HPA002381; -.
DR MIM; 602463; gene.
DR neXtProt; NX_Q16555; -.
DR PharmGKB; PA27472; -.
DR eggNOG; COG0044; -.
DR HOVERGEN; HBG000806; -.
DR InParanoid; Q16555; -.
DR KO; K07528; -.
DR OMA; EVPAFFK; -.
DR OrthoDB; EOG7SJD48; -.
DR PhylomeDB; Q16555; -.
DR Reactome; REACT_111045; Developmental Biology.
DR ChiTaRS; DPYSL2; human.
DR EvolutionaryTrace; Q16555; -.
DR GeneWiki; DPYSL2; -.
DR GenomeRNAi; 1808; -.
DR NextBio; 7369; -.
DR PRO; PR:Q16555; -.
DR ArrayExpress; Q16555; -.
DR Bgee; Q16555; -.
DR CleanEx; HS_DPYSL2; -.
DR Genevestigator; Q16555; -.
DR GO; GO:0030424; C:axon; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0030425; C:dendrite; IEA:Ensembl.
DR GO; GO:0030426; C:growth cone; IEA:Ensembl.
DR GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR GO; GO:0043025; C:neuronal cell body; IEA:Ensembl.
DR GO; GO:0043234; C:protein complex; IEA:Ensembl.
DR GO; GO:0004157; F:dihydropyrimidinase activity; TAS:ProtInc.
DR GO; GO:0007411; P:axon guidance; TAS:Reactome.
DR GO; GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:0006897; P:endocytosis; IMP:UniProtKB.
DR GO; GO:0006139; P:nucleobase-containing compound metabolic process; TAS:ProtInc.
DR GO; GO:0021772; P:olfactory bulb development; IEA:Ensembl.
DR GO; GO:0014049; P:positive regulation of glutamate secretion; IEA:Ensembl.
DR GO; GO:0006208; P:pyrimidine nucleobase catabolic process; IEA:InterPro.
DR GO; GO:0045664; P:regulation of neuron differentiation; IEA:Ensembl.
DR GO; GO:0001975; P:response to amphetamine; IEA:Ensembl.
DR GO; GO:0042220; P:response to cocaine; IEA:Ensembl.
DR GO; GO:0042493; P:response to drug; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR GO; GO:0021510; P:spinal cord development; IEA:Ensembl.
DR GO; GO:0048489; P:synaptic vesicle transport; IEA:Ensembl.
DR InterPro; IPR006680; Amidohydro_1.
DR InterPro; IPR011778; Hydantoinase/dihydroPyrase.
DR InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR Pfam; PF01979; Amidohydro_1; 1.
DR SUPFAM; SSF51338; SSF51338; 2.
DR TIGRFAMs; TIGR02033; D-hydantoinase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Complete proteome; Cytoplasm;
KW Cytoskeleton; Developmental protein; Differentiation;
KW Direct protein sequencing; Membrane; Neurogenesis; Phosphoprotein;
KW Polymorphism; Reference proteome.
FT CHAIN 1 572 Dihydropyrimidinase-related protein 2.
FT /FTId=PRO_0000165913.
FT MOD_RES 32 32 Phosphotyrosine; by FYN.
FT MOD_RES 431 431 Phosphotyrosine (By similarity).
FT MOD_RES 465 465 Phosphoserine (By similarity).
FT MOD_RES 499 499 Phosphotyrosine (By similarity).
FT MOD_RES 509 509 Phosphothreonine.
FT MOD_RES 514 514 Phosphothreonine; by GSK3-beta.
FT MOD_RES 517 517 Phosphoserine.
FT MOD_RES 518 518 Phosphoserine.
FT MOD_RES 522 522 Phosphoserine; by DYRK2.
FT MOD_RES 555 555 Phosphothreonine; by ROCK2 (By
FT similarity).
FT VAR_SEQ 1 36 Missing (in isoform 2).
FT /FTId=VSP_044941.
FT VARIANT 118 118 A -> T (in dbSNP:rs2289593).
FT /FTId=VAR_022016.
FT VARIANT 481 481 R -> C (in a colorectal cancer sample;
FT somatic mutation).
FT /FTId=VAR_036316.
FT MUTAGEN 71 71 D->N: Inhibits axon outgrowth formation
FT in hippocampal neurons and decreases
FT binding to CYFIP1.
FT MUTAGEN 507 507 S->A: No effect.
FT MUTAGEN 509 509 T->A: Greatly diminishes binding to 3F4
FT antibody.
FT MUTAGEN 512 512 T->A: No effect.
FT MUTAGEN 514 514 T->A: No effect.
FT MUTAGEN 517 517 S->A: No effect.
FT MUTAGEN 518 518 S->A: Greatly diminishes binding to 3F4
FT antibody.
FT MUTAGEN 521 521 T->A: No effect.
FT MUTAGEN 522 522 S->A: Greatly diminishes binding to 3F4
FT antibody.
FT STRAND 17 25
FT STRAND 30 38
FT STRAND 41 48
FT STRAND 53 55
FT STRAND 57 59
FT STRAND 64 67
FT STRAND 69 74
FT HELIX 89 98
FT STRAND 101 108
FT HELIX 116 130
FT STRAND 132 140
FT HELIX 146 158
FT STRAND 163 170
FT TURN 171 173
FT HELIX 178 191
FT STRAND 194 198
FT HELIX 202 213
FT TURN 214 216
FT HELIX 221 226
FT HELIX 229 246
FT STRAND 250 255
FT HELIX 258 269
FT STRAND 274 279
FT HELIX 280 284
FT HELIX 287 291
FT HELIX 295 300
FT HELIX 313 322
FT HELIX 338 341
FT HELIX 342 344
FT HELIX 348 350
FT TURN 358 360
FT HELIX 361 369
FT TURN 370 373
FT HELIX 377 384
FT HELIX 386 392
FT TURN 395 397
FT STRAND 409 419
FT TURN 422 424
FT STRAND 425 428
FT TURN 433 436
FT STRAND 438 448
FT STRAND 451 455
FT HELIX 476 487
SQ SEQUENCE 572 AA; 62294 MW; 5CDB6CF7F5C308AD CRC64;
MSYQGKKNIP RITSDRLLIK GGKIVNDDQS FYADIYMEDG LIKQIGENLI VPGGVKTIEA
HSRMVIPGGI DVHTRFQMPD QGMTSADDFF QGTKAALAGG TTMIIDHVVP EPGTSLLAAF
DQWREWADSK SCCDYSLHVD ISEWHKGIQE EMEALVKDHG VNSFLVYMAF KDRFQLTDCQ
IYEVLSVIRD IGAIAQVHAE NGDIIAEEQQ RILDLGITGP EGHVLSRPEE VEAEAVNRAI
TIANQTNCPL YITKVMSKSS AEVIAQARKK GTVVYGEPIT ASLGTDGSHY WSKNWAKAAA
FVTSPPLSPD PTTPDFLNSL LSCGDLQVTG SAHCTFNTAQ KAVGKDNFTL IPEGTNGTEE
RMSVIWDKAV VTGKMDENQF VAVTSTNAAK VFNLYPRKGR IAVGSDADLV IWDPDSVKTI
SAKTHNSSLE YNIFEGMECR GSPLVVISQG KIVLEDGTLH VTEGSGRYIP RKPFPDFVYK
RIKARSRLAE LRGVPRGLYD GPVCEVSVTP KTVTPASSAK TSPAKQQAPP VRNLHQSGFS
LSGAQIDDNI PRRTTQRIVA PPGGRANITS LG
//
ID DPYL2_HUMAN Reviewed; 572 AA.
AC Q16555; A8K5H2; B4DR31; D3DSS7; O00424;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1996, sequence version 1.
DT 22-JAN-2014, entry version 140.
DE RecName: Full=Dihydropyrimidinase-related protein 2;
DE Short=DRP-2;
DE AltName: Full=Collapsin response mediator protein 2;
DE Short=CRMP-2;
DE AltName: Full=N2A3;
DE AltName: Full=Unc-33-like phosphoprotein 2;
DE Short=ULIP-2;
GN Name=DPYSL2; Synonyms=CRMP2, ULIP2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=7637782; DOI=10.1038/376509a0;
RA Goshima Y., Nakamura F., Strittmatter P., Strittmatter S.M.;
RT "Collapsin-induced growth cone collapse mediated by an intracellular
RT protein related to UNC-33.";
RL Nature 376:509-514(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=8973361; DOI=10.1016/S0378-1119(96)00445-3;
RA Hamajima N., Matsuda K., Sakata S., Tamaki N., Sasaki M., Nonaka M.;
RT "A novel gene family defined by human dihydropyrimidinase and three
RT related proteins with differential tissue distribution.";
RL Gene 180:157-163(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Fetal liver;
RA Zhou J., Chen Y., Gu J.R.;
RT "A cDNA clone highly expressed in human brain and deleted in liver
RT cancer.";
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10574455; DOI=10.1093/dnares/6.5.291;
RA Kitamura K., Takayama M., Hamajima N., Nakanishi M., Sasaki M.,
RA Endo Y., Takemoto T., Kimura H., Iwaki M., Nonaka M.;
RT "Characterization of the human dihydropyrimidinase-related protein 2
RT (DRP-2) gene.";
RL DNA Res. 6:291-297(1999).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S.,
RA Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A.,
RA Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA Allen N.R., Anderson S., Asakawa T., Blechschmidt K., Bloom T.,
RA Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K.,
RA DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G.,
RA Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B.,
RA Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C.,
RA O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K.,
RA Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R.,
RA Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K.,
RA Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q.,
RA Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N.,
RA Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Eye, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP PROTEIN SEQUENCE OF 44-56; 64-75; 147-157; 174-211; 239-254; 375-390;
RP 401-418; 424-467; 497-511 AND 533-552, AND MASS SPECTROMETRY.
RC TISSUE=Brain, Cajal-Retzius cell, and Fetal brain cortex;
RA Lubec G., Afjehi-Sadat L., Chen W.-Q., Sun Y.;
RL Submitted (DEC-2008) to UniProtKB.
RN [10]
RP PHOSPHORYLATION AT SER-518; SER-522 AND THR-509, AND MUTAGENESIS OF
RP SER-507; THR-509; THR-512; THR-514; SER-517; SER-518; THR-521 AND
RP SER-522.
RX PubMed=10757975; DOI=10.1021/bi992323h;
RA Gu Y., Hamajima N., Ihara Y.;
RT "Neurofibrillary tangle-associated collapsin response mediator
RT protein-2 (CRMP-2) is highly phosphorylated on Thr-509, Ser-518, and
RT Ser-522.";
RL Biochemistry 39:4267-4275(2000).
RN [11]
RP FUNCTION.
RX PubMed=11477421; DOI=10.1038/90476;
RA Inagaki N., Chihara K., Arimura N., Menager C., Kawano Y., Matsuo N.,
RA Nishimura T., Amano M., Kaibuchi K.;
RT "CRMP-2 induces axons in cultured hippocampal neurons.";
RL Nat. Neurosci. 4:781-782(2001).
RN [12]
RP FUNCTION, AND PHOSPHORYLATION AT SER-522.
RX PubMed=15466863; DOI=10.1074/jbc.C400412200;
RA Cole A.R., Knebel A., Morrice N.A., Robertson L.A., Irving A.J.,
RA Connolly C.N., Sutherland C.;
RT "GSK-3 phosphorylation of the Alzheimer epitope within collapsin
RT response mediator proteins regulates axon elongation in primary
RT neurons.";
RL J. Biol. Chem. 279:50176-50180(2004).
RN [13]
RP INTERACTION WITH CYFIP1, AND MUTAGENESIS OF ASP-71.
RX PubMed=16260607; DOI=10.1128/MCB.25.22.9920-9935.2005;
RA Kawano Y., Yoshimura T., Tsuboi D., Kawabata S., Kaneko-Kawano T.,
RA Shirataki H., Takenawa T., Kaibuchi K.;
RT "CRMP-2 is involved in kinesin-1-dependent transport of the Sra-
RT 1/WAVE1 complex and axon formation.";
RL Mol. Cell. Biol. 25:9920-9935(2005).
RN [14]
RP INTERACTION WITH PLEXNA1, AND SUBUNIT.
RX PubMed=14685275; DOI=10.1038/sj.emboj.7600021;
RA Deo R.C., Schmidt E.F., Elhabazi A., Togashi H., Burley S.K.,
RA Strittmatter S.M.;
RT "Structural bases for CRMP function in plexin-dependent semaphorin3A
RT signaling.";
RL EMBO J. 23:9-22(2004).
RN [15]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-509 AND SER-522, AND
RP MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=16964243; DOI=10.1038/nbt1240;
RA Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
RT "A probability-based approach for high-throughput protein
RT phosphorylation analysis and site localization.";
RL Nat. Biotechnol. 24:1285-1292(2006).
RN [16]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-509, AND MASS
RP SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [17]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [18]
RP PHOSPHORYLATION AT TYR-32 BY FYN.
RX PubMed=19652227; DOI=10.1074/jbc.M109.000240;
RA Uchida Y., Ohshima T., Yamashita N., Ogawara M., Sasaki Y.,
RA Nakamura F., Goshima Y.;
RT "Semaphorin3A signaling mediated by Fyn-dependent tyrosine
RT phosphorylation of collapsin response mediator protein 2 at tyrosine
RT 32.";
RL J. Biol. Chem. 284:27393-27401(2009).
RN [19]
RP INTERACTION WITH CLN6.
RX PubMed=19235893; DOI=10.1002/jnr.22032;
RA Benedict J.W., Getty A.L., Wishart T.M., Gillingwater T.H.,
RA Pearce D.A.;
RT "Protein product of CLN6 gene responsible for variant late-onset
RT infantile neuronal ceroid lipofuscinosis interacts with CRMP-2.";
RL J. Neurosci. Res. 87:2157-2166(2009).
RN [20]
RP FUNCTION IN ENDOCYTOSIS, INTERACTION WITH MICALL1, AND SUBCELLULAR
RP LOCATION.
RX PubMed=20801876; DOI=10.1074/jbc.C110.166066;
RA Rahajeng J., Giridharan S.S., Naslavsky N., Caplan S.;
RT "Collapsin response mediator protein-2 (Crmp2) regulates trafficking
RT by linking endocytic regulatory proteins to dynein motors.";
RL J. Biol. Chem. 285:31918-31922(2010).
RN [21]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full
RT phosphorylation site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [22]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [23]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-509; THR-514; SER-517;
RP SER-518 AND SER-522, AND MASS SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [24]
RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 12-490, AND SUBUNIT.
RX PubMed=17250651; DOI=10.1111/j.1471-4159.2006.04401.x;
RA Stenmark P., Ogg D., Flodin S., Flores A., Kotenyova T., Nyman T.,
RA Nordlund P., Kursula P.;
RT "The structure of human collapsin response mediator protein 2, a
RT regulator of axonal growth.";
RL J. Neurochem. 101:906-917(2007).
RN [25]
RP VARIANT [LARGE SCALE ANALYSIS] CYS-481.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S.,
RA Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J.,
RA Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C.,
RA Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N.,
RA Vogelstein B., Kinzler K.W., Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal
RT cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Plays a role in neuronal development and polarity, as
CC well as in axon growth and guidance, neuronal growth cone collapse
CC and cell migration. Necessary for signaling by class 3 semaphorins
CC and subsequent remodeling of the cytoskeleton. May play a role in
CC endocytosis.
CC -!- SUBUNIT: Homotetramer, and heterotetramer with CRMP1, DPYSL3,
CC DPYSL4 or DPYSL5. Interacts through its C-terminus with the C-
CC terminus of CYFIP1/SRA1. Interacts with HTR4. Interacts with CLN6.
CC Interacts with MICALL1.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasm, cytoskeleton.
CC Membrane. Note=Tightly but non-covalently associated with
CC membranes.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q16555-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q16555-2; Sequence=VSP_044941;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Ubiquitous.
CC -!- PTM: 3F4, a monoclonal antibody which strongly stains
CC neurofibrillary tangles in Alzheimer disease brains, specifically
CC labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
CC -!- PTM: Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding
CC leading to destabilization of microtubule assembly in axons and
CC neurodegeneration (By similarity). Phosphorylation by DYRK2 at
CC Ser-522 is required for subsequent phosphorylation by GSK3B.
CC -!- SIMILARITY: Belongs to the DHOase family.
CC Hydantoinase/dihydropyrimidinase subfamily.
CC -!- CAUTION: Lacks most of the conserved residues that are essential
CC for binding the metal cofactor and hence for dihydropyrimidinase
CC activity. Its enzyme activity is therefore unsure.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; U17279; AAA93202.1; -; mRNA.
DR EMBL; D78013; BAA11191.1; -; mRNA.
DR EMBL; U97105; AAC05793.1; -; mRNA.
DR EMBL; AB020777; BAA86991.1; -; Genomic_DNA.
DR EMBL; AK291287; BAF83976.1; -; mRNA.
DR EMBL; AK299077; BAG61143.1; -; mRNA.
DR EMBL; AC015564; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC015743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471080; EAW63573.1; -; Genomic_DNA.
DR EMBL; CH471080; EAW63574.1; -; Genomic_DNA.
DR EMBL; BC056408; AAH56408.1; -; mRNA.
DR EMBL; BC067109; AAH67109.1; -; mRNA.
DR PIR; JC5317; JC5317.
DR RefSeq; NP_001184222.1; NM_001197293.2.
DR RefSeq; NP_001231533.1; NM_001244604.1.
DR RefSeq; NP_001377.1; NM_001386.5.
DR UniGene; Hs.593187; -.
DR PDB; 2GSE; X-ray; 2.40 A; A/B/C/D=13-490.
DR PDB; 2VM8; X-ray; 1.90 A; A/B/C/D=13-490.
DR PDBsum; 2GSE; -.
DR PDBsum; 2VM8; -.
DR ProteinModelPortal; Q16555; -.
DR SMR; Q16555; 14-490.
DR IntAct; Q16555; 13.
DR MINT; MINT-3032752; -.
DR STRING; 9606.ENSP00000309539; -.
DR MEROPS; M38.975; -.
DR PhosphoSite; Q16555; -.
DR DMDM; 3122051; -.
DR REPRODUCTION-2DPAGE; IPI00257508; -.
DR REPRODUCTION-2DPAGE; Q16555; -.
DR UCD-2DPAGE; Q16555; -.
DR PaxDb; Q16555; -.
DR PeptideAtlas; Q16555; -.
DR PRIDE; Q16555; -.
DR Ensembl; ENST00000311151; ENSP00000309539; ENSG00000092964.
DR Ensembl; ENST00000521913; ENSP00000427985; ENSG00000092964.
DR Ensembl; ENST00000523027; ENSP00000431117; ENSG00000092964.
DR GeneID; 1808; -.
DR KEGG; hsa:1808; -.
DR UCSC; uc003xfb.2; human.
DR CTD; 1808; -.
DR GeneCards; GC08P026371; -.
DR HGNC; HGNC:3014; DPYSL2.
DR HPA; CAB018719; -.
DR HPA; HPA002381; -.
DR MIM; 602463; gene.
DR neXtProt; NX_Q16555; -.
DR PharmGKB; PA27472; -.
DR eggNOG; COG0044; -.
DR HOVERGEN; HBG000806; -.
DR InParanoid; Q16555; -.
DR KO; K07528; -.
DR OMA; EVPAFFK; -.
DR OrthoDB; EOG7SJD48; -.
DR PhylomeDB; Q16555; -.
DR Reactome; REACT_111045; Developmental Biology.
DR ChiTaRS; DPYSL2; human.
DR EvolutionaryTrace; Q16555; -.
DR GeneWiki; DPYSL2; -.
DR GenomeRNAi; 1808; -.
DR NextBio; 7369; -.
DR PRO; PR:Q16555; -.
DR ArrayExpress; Q16555; -.
DR Bgee; Q16555; -.
DR CleanEx; HS_DPYSL2; -.
DR Genevestigator; Q16555; -.
DR GO; GO:0030424; C:axon; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0030425; C:dendrite; IEA:Ensembl.
DR GO; GO:0030426; C:growth cone; IEA:Ensembl.
DR GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR GO; GO:0043025; C:neuronal cell body; IEA:Ensembl.
DR GO; GO:0043234; C:protein complex; IEA:Ensembl.
DR GO; GO:0004157; F:dihydropyrimidinase activity; TAS:ProtInc.
DR GO; GO:0007411; P:axon guidance; TAS:Reactome.
DR GO; GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:0006897; P:endocytosis; IMP:UniProtKB.
DR GO; GO:0006139; P:nucleobase-containing compound metabolic process; TAS:ProtInc.
DR GO; GO:0021772; P:olfactory bulb development; IEA:Ensembl.
DR GO; GO:0014049; P:positive regulation of glutamate secretion; IEA:Ensembl.
DR GO; GO:0006208; P:pyrimidine nucleobase catabolic process; IEA:InterPro.
DR GO; GO:0045664; P:regulation of neuron differentiation; IEA:Ensembl.
DR GO; GO:0001975; P:response to amphetamine; IEA:Ensembl.
DR GO; GO:0042220; P:response to cocaine; IEA:Ensembl.
DR GO; GO:0042493; P:response to drug; IEA:Ensembl.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR GO; GO:0021510; P:spinal cord development; IEA:Ensembl.
DR GO; GO:0048489; P:synaptic vesicle transport; IEA:Ensembl.
DR InterPro; IPR006680; Amidohydro_1.
DR InterPro; IPR011778; Hydantoinase/dihydroPyrase.
DR InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR Pfam; PF01979; Amidohydro_1; 1.
DR SUPFAM; SSF51338; SSF51338; 2.
DR TIGRFAMs; TIGR02033; D-hydantoinase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Complete proteome; Cytoplasm;
KW Cytoskeleton; Developmental protein; Differentiation;
KW Direct protein sequencing; Membrane; Neurogenesis; Phosphoprotein;
KW Polymorphism; Reference proteome.
FT CHAIN 1 572 Dihydropyrimidinase-related protein 2.
FT /FTId=PRO_0000165913.
FT MOD_RES 32 32 Phosphotyrosine; by FYN.
FT MOD_RES 431 431 Phosphotyrosine (By similarity).
FT MOD_RES 465 465 Phosphoserine (By similarity).
FT MOD_RES 499 499 Phosphotyrosine (By similarity).
FT MOD_RES 509 509 Phosphothreonine.
FT MOD_RES 514 514 Phosphothreonine; by GSK3-beta.
FT MOD_RES 517 517 Phosphoserine.
FT MOD_RES 518 518 Phosphoserine.
FT MOD_RES 522 522 Phosphoserine; by DYRK2.
FT MOD_RES 555 555 Phosphothreonine; by ROCK2 (By
FT similarity).
FT VAR_SEQ 1 36 Missing (in isoform 2).
FT /FTId=VSP_044941.
FT VARIANT 118 118 A -> T (in dbSNP:rs2289593).
FT /FTId=VAR_022016.
FT VARIANT 481 481 R -> C (in a colorectal cancer sample;
FT somatic mutation).
FT /FTId=VAR_036316.
FT MUTAGEN 71 71 D->N: Inhibits axon outgrowth formation
FT in hippocampal neurons and decreases
FT binding to CYFIP1.
FT MUTAGEN 507 507 S->A: No effect.
FT MUTAGEN 509 509 T->A: Greatly diminishes binding to 3F4
FT antibody.
FT MUTAGEN 512 512 T->A: No effect.
FT MUTAGEN 514 514 T->A: No effect.
FT MUTAGEN 517 517 S->A: No effect.
FT MUTAGEN 518 518 S->A: Greatly diminishes binding to 3F4
FT antibody.
FT MUTAGEN 521 521 T->A: No effect.
FT MUTAGEN 522 522 S->A: Greatly diminishes binding to 3F4
FT antibody.
FT STRAND 17 25
FT STRAND 30 38
FT STRAND 41 48
FT STRAND 53 55
FT STRAND 57 59
FT STRAND 64 67
FT STRAND 69 74
FT HELIX 89 98
FT STRAND 101 108
FT HELIX 116 130
FT STRAND 132 140
FT HELIX 146 158
FT STRAND 163 170
FT TURN 171 173
FT HELIX 178 191
FT STRAND 194 198
FT HELIX 202 213
FT TURN 214 216
FT HELIX 221 226
FT HELIX 229 246
FT STRAND 250 255
FT HELIX 258 269
FT STRAND 274 279
FT HELIX 280 284
FT HELIX 287 291
FT HELIX 295 300
FT HELIX 313 322
FT HELIX 338 341
FT HELIX 342 344
FT HELIX 348 350
FT TURN 358 360
FT HELIX 361 369
FT TURN 370 373
FT HELIX 377 384
FT HELIX 386 392
FT TURN 395 397
FT STRAND 409 419
FT TURN 422 424
FT STRAND 425 428
FT TURN 433 436
FT STRAND 438 448
FT STRAND 451 455
FT HELIX 476 487
SQ SEQUENCE 572 AA; 62294 MW; 5CDB6CF7F5C308AD CRC64;
MSYQGKKNIP RITSDRLLIK GGKIVNDDQS FYADIYMEDG LIKQIGENLI VPGGVKTIEA
HSRMVIPGGI DVHTRFQMPD QGMTSADDFF QGTKAALAGG TTMIIDHVVP EPGTSLLAAF
DQWREWADSK SCCDYSLHVD ISEWHKGIQE EMEALVKDHG VNSFLVYMAF KDRFQLTDCQ
IYEVLSVIRD IGAIAQVHAE NGDIIAEEQQ RILDLGITGP EGHVLSRPEE VEAEAVNRAI
TIANQTNCPL YITKVMSKSS AEVIAQARKK GTVVYGEPIT ASLGTDGSHY WSKNWAKAAA
FVTSPPLSPD PTTPDFLNSL LSCGDLQVTG SAHCTFNTAQ KAVGKDNFTL IPEGTNGTEE
RMSVIWDKAV VTGKMDENQF VAVTSTNAAK VFNLYPRKGR IAVGSDADLV IWDPDSVKTI
SAKTHNSSLE YNIFEGMECR GSPLVVISQG KIVLEDGTLH VTEGSGRYIP RKPFPDFVYK
RIKARSRLAE LRGVPRGLYD GPVCEVSVTP KTVTPASSAK TSPAKQQAPP VRNLHQSGFS
LSGAQIDDNI PRRTTQRIVA PPGGRANITS LG
//
MIM
602463
*RECORD*
*FIELD* NO
602463
*FIELD* TI
*602463 DIHYDROPYRIMIDINASE-LIKE 2; DPYSL2
;;DIHYDROPYRIMIDINASE-RELATED PROTEIN 2; DRP2;;
read moreCOLLAPSIN RESPONSE MEDIATOR PROTEIN 2; CRMP2
*FIELD* TX
DESCRIPTION
The DPYSL2 gene encodes a cytosolic phosphoprotein that can act as a
mediator of growth cone collapse as well as modify axon number, length,
and neuronal polarity (summary by Brittain et al., 2011).
CLONING
Hamajima et al. (1996) isolated a human cDNA encoding
dihydropyrimidinase-like 2 (DPYSL2), called DRP2 by them, from a fetal
brain cDNA library (see 222748). The DPYSL2 protein has 572 amino acids.
Northern blot analysis detected a 4.9-kb DPYSL2 transcript in all
tissues examined except liver.
GENE FUNCTION
Using several mammalian cell lines and expression constructs, Fukata et
al. (2002) found that Crmp2 preferentially bound tubulin (see 602529)
dimers rather than microtubules. The binding property of Crmp2 with
tubulin was distinct from that of Tau (157140). In neurons,
overexpression of Crmp2 promoted axonal growth and branching. A Crmp2
mutant lacking the region responsible for microtubule assembly inhibited
axonal growth and branching in a dominant-negative manner. Fukata et al.
(2002) concluded that Crmp2 regulates axonal growth and branching as a
partner of the tubulin heterodimer in a manner that differs from other
microtubule-associated proteins.
Yoshimura et al. (2005) showed that GSK3-beta (605004) phosphorylated
CRMP2 at thr514 and inactivated it. Expression of the nonphosphorylated
form of CRMP2 or inhibition of GSK3-beta induced the formation of
multiple axon-like neurites in hippocampal neurons. Expression of
constitutively active GSK3-beta impaired neuronal polarization, whereas
the nonphosphorylated form of CRMP2 counteracted the inhibitory effects
of GSK3-beta, indicating that GSK3-beta regulates neuronal polarity
through phosphorylation of CRMP2. Treatment of hippocampal neurons with
neurotrophin-3 (NT3; 162660) induced inactivation of GSK3-beta and
dephosphorylation of CRMP2. Knockdown of CRMP2 inhibited NT3-induced
axon outgrowth. These results suggested that NT3 decreases
phosphorylated CRMP2 and increases nonphosphorylated active CRMP2,
thereby promoting axon outgrowth.
Using RT-PCR, flow cytometry, and Western blot analysis, Vincent et al.
(2005) found that CRMP2 was expressed in neural and T-lymphocyte cell
lines, CD4- and CD8-positive peripheral blood T cells, monocytes, and B
cells. Immunofluorescence microscopy showed that CRMP2 expression
redistributed to the uropod of IL2 (147680)-polarized T cells. Blockade
of CRMP2 expression with short interfering RNA inhibited chemokine- but
not semaphorin-directed lymphocyte transmigration. Patients affected
with HTLV-1-associated myelopathy (HAM; 159580) had increased
T-lymphocyte transmigration activity and increased expression of CRMP2.
Vincent et al. (2005) proposed that CRMP2 has a role in pathogenesis of
neuroinflammatory disease.
CRMP2 interacts with and modulates the CaV2.2 calcium channel (CACNA1B;
601012). Overexpression of CRMP2 leads to increased surface expression
of CaV2.2 on neurons, enhanced calcium currents, and an increase in
stimulated release of the neuropeptide calcitonin gene-related peptide
(CGRP; 114130) from dorsal root ganglia (summary by Brittain et al.,
2011).
MAPPING
Hamajima et al. (1996) noted that 3 ESTs mapped to chromosome 8p21 by
Koyama et al. (1995) correspond to a portion of the coding region of
DPYSL2.
ANIMAL MODEL
In rats, Brittain et al. (2011) demonstrated that inflammatory and
neuropathic hypersensitivity can be suppressed by a synthetic 15-mer
peptide (CBD3) that uncouples Crmp2 and CaV2.2 (CACNA1B; 601012) calcium
channels in neurons. Studies in cultured neurons and spinal cord slices
showed that CBD3 peptide bound CaV2.2, interfered with the interaction
of Crmp2 and CaV2.2, and reduced channel function, as evidenced by
decreased CGRP neuropeptide release from sensory neurons and decreased
excitatory synaptic transmission in dorsal horn neurons. In rats,
treatment with CBD3 also reduced meningeal blood flow and reduced
nocifensive behavior induced by peripheral formalin injection or corneal
capsaicin application, as well as reversed neuropathic hypersensitivity
produced by an antiretroviral drug. The peptide was mildly anxiolytic
without affecting memory retrieval, sensorimotor function or depression.
*FIELD* RF
1. Brittain, J. M.; Duarte, D. B.; Wilson, S. M.; Zhu, W.; Ballard,
C.; Johnson, P. L.; Liu, N.; Xiong, W.; Ripsch, M. S.; Wang, Y.; Fehrenbacher,
J. C.; Fitz, S. D.; and 20 others: Suppression of inflammatory
and neuropathic pain by uncoupling CRMP-2 from the presynaptic Ca(2+)
channel complex. Nature Med. 17: 822-829, 2011.
2. Fukata, Y.; Itoh, T. J.; Kimura, T.; Menager, C.; Nishimura, T.;
Shiromizu, T.; Watanabe, H.; Inagaki, N.; Iwamatsu, A.; Hotani, H.;
Kaibuchi, K.: CRMP-2 binds to tubulin heterodimers to promote microtubule
assembly. Nature Cell Biol. 4: 583-591, 2002.
3. Hamajima, N.; Matsuda, K.; Sakata, S.; Tamaki, N.; Sasaki, M.;
Nonaka, M.: A novel gene family defined by human dihydropyrimidinase
and three related proteins with differential tissue distribution. Gene 180:
157-163, 1996.
4. Koyama, K.; Sudo, K.; Nakamura, Y.: Isolation of 115 human chromosome
8-specific expressed-sequence tags by exon amplification. Genomics 26:
245-253, 1995.
5. Vincent, P.; Collette, Y.; Marignier, R.; Vuaillat, C.; Rogemond,
V.; Davoust, N.; Malcus, C.; Cavagna, S.; Gessain, A.; Machuca-Gayet,
I.; Belin, M.-F.; Quach, T.; Giraudon, P.: A role for the neuronal
protein collapsin response mediator protein 2 in T lymphocyte polarization
and migration. J. Immun. 175: 7650-7660, 2005.
6. Yoshimura, T.; Kawano, Y.; Arimura, N.; Kawabata, S.; Kikuchi,
A.; Kaibuchi, K.: GSK-3-beta regulates phosphorylation of CRMP-2
and neuronal polarity. Cell 120: 137-149, 2005.
*FIELD* CN
Cassandra L. Kniffin - updated: 8/3/2011
Paul J. Converse - updated: 8/4/2006
Stylianos E. Antonarakis - updated: 1/24/2005
Patricia A. Hartz - updated: 12/16/2002
Patti M. Sherman - updated: 4/23/1998
*FIELD* CD
Patti M. Sherman: 3/20/1998
*FIELD* ED
wwang: 08/05/2011
ckniffin: 8/3/2011
mgross: 8/30/2006
terry: 8/4/2006
mgross: 1/24/2005
mgross: 12/18/2002
terry: 12/16/2002
psherman: 4/23/1998
psherman: 3/24/1998
psherman: 3/23/1998
psherman: 3/20/1998
*RECORD*
*FIELD* NO
602463
*FIELD* TI
*602463 DIHYDROPYRIMIDINASE-LIKE 2; DPYSL2
;;DIHYDROPYRIMIDINASE-RELATED PROTEIN 2; DRP2;;
read moreCOLLAPSIN RESPONSE MEDIATOR PROTEIN 2; CRMP2
*FIELD* TX
DESCRIPTION
The DPYSL2 gene encodes a cytosolic phosphoprotein that can act as a
mediator of growth cone collapse as well as modify axon number, length,
and neuronal polarity (summary by Brittain et al., 2011).
CLONING
Hamajima et al. (1996) isolated a human cDNA encoding
dihydropyrimidinase-like 2 (DPYSL2), called DRP2 by them, from a fetal
brain cDNA library (see 222748). The DPYSL2 protein has 572 amino acids.
Northern blot analysis detected a 4.9-kb DPYSL2 transcript in all
tissues examined except liver.
GENE FUNCTION
Using several mammalian cell lines and expression constructs, Fukata et
al. (2002) found that Crmp2 preferentially bound tubulin (see 602529)
dimers rather than microtubules. The binding property of Crmp2 with
tubulin was distinct from that of Tau (157140). In neurons,
overexpression of Crmp2 promoted axonal growth and branching. A Crmp2
mutant lacking the region responsible for microtubule assembly inhibited
axonal growth and branching in a dominant-negative manner. Fukata et al.
(2002) concluded that Crmp2 regulates axonal growth and branching as a
partner of the tubulin heterodimer in a manner that differs from other
microtubule-associated proteins.
Yoshimura et al. (2005) showed that GSK3-beta (605004) phosphorylated
CRMP2 at thr514 and inactivated it. Expression of the nonphosphorylated
form of CRMP2 or inhibition of GSK3-beta induced the formation of
multiple axon-like neurites in hippocampal neurons. Expression of
constitutively active GSK3-beta impaired neuronal polarization, whereas
the nonphosphorylated form of CRMP2 counteracted the inhibitory effects
of GSK3-beta, indicating that GSK3-beta regulates neuronal polarity
through phosphorylation of CRMP2. Treatment of hippocampal neurons with
neurotrophin-3 (NT3; 162660) induced inactivation of GSK3-beta and
dephosphorylation of CRMP2. Knockdown of CRMP2 inhibited NT3-induced
axon outgrowth. These results suggested that NT3 decreases
phosphorylated CRMP2 and increases nonphosphorylated active CRMP2,
thereby promoting axon outgrowth.
Using RT-PCR, flow cytometry, and Western blot analysis, Vincent et al.
(2005) found that CRMP2 was expressed in neural and T-lymphocyte cell
lines, CD4- and CD8-positive peripheral blood T cells, monocytes, and B
cells. Immunofluorescence microscopy showed that CRMP2 expression
redistributed to the uropod of IL2 (147680)-polarized T cells. Blockade
of CRMP2 expression with short interfering RNA inhibited chemokine- but
not semaphorin-directed lymphocyte transmigration. Patients affected
with HTLV-1-associated myelopathy (HAM; 159580) had increased
T-lymphocyte transmigration activity and increased expression of CRMP2.
Vincent et al. (2005) proposed that CRMP2 has a role in pathogenesis of
neuroinflammatory disease.
CRMP2 interacts with and modulates the CaV2.2 calcium channel (CACNA1B;
601012). Overexpression of CRMP2 leads to increased surface expression
of CaV2.2 on neurons, enhanced calcium currents, and an increase in
stimulated release of the neuropeptide calcitonin gene-related peptide
(CGRP; 114130) from dorsal root ganglia (summary by Brittain et al.,
2011).
MAPPING
Hamajima et al. (1996) noted that 3 ESTs mapped to chromosome 8p21 by
Koyama et al. (1995) correspond to a portion of the coding region of
DPYSL2.
ANIMAL MODEL
In rats, Brittain et al. (2011) demonstrated that inflammatory and
neuropathic hypersensitivity can be suppressed by a synthetic 15-mer
peptide (CBD3) that uncouples Crmp2 and CaV2.2 (CACNA1B; 601012) calcium
channels in neurons. Studies in cultured neurons and spinal cord slices
showed that CBD3 peptide bound CaV2.2, interfered with the interaction
of Crmp2 and CaV2.2, and reduced channel function, as evidenced by
decreased CGRP neuropeptide release from sensory neurons and decreased
excitatory synaptic transmission in dorsal horn neurons. In rats,
treatment with CBD3 also reduced meningeal blood flow and reduced
nocifensive behavior induced by peripheral formalin injection or corneal
capsaicin application, as well as reversed neuropathic hypersensitivity
produced by an antiretroviral drug. The peptide was mildly anxiolytic
without affecting memory retrieval, sensorimotor function or depression.
*FIELD* RF
1. Brittain, J. M.; Duarte, D. B.; Wilson, S. M.; Zhu, W.; Ballard,
C.; Johnson, P. L.; Liu, N.; Xiong, W.; Ripsch, M. S.; Wang, Y.; Fehrenbacher,
J. C.; Fitz, S. D.; and 20 others: Suppression of inflammatory
and neuropathic pain by uncoupling CRMP-2 from the presynaptic Ca(2+)
channel complex. Nature Med. 17: 822-829, 2011.
2. Fukata, Y.; Itoh, T. J.; Kimura, T.; Menager, C.; Nishimura, T.;
Shiromizu, T.; Watanabe, H.; Inagaki, N.; Iwamatsu, A.; Hotani, H.;
Kaibuchi, K.: CRMP-2 binds to tubulin heterodimers to promote microtubule
assembly. Nature Cell Biol. 4: 583-591, 2002.
3. Hamajima, N.; Matsuda, K.; Sakata, S.; Tamaki, N.; Sasaki, M.;
Nonaka, M.: A novel gene family defined by human dihydropyrimidinase
and three related proteins with differential tissue distribution. Gene 180:
157-163, 1996.
4. Koyama, K.; Sudo, K.; Nakamura, Y.: Isolation of 115 human chromosome
8-specific expressed-sequence tags by exon amplification. Genomics 26:
245-253, 1995.
5. Vincent, P.; Collette, Y.; Marignier, R.; Vuaillat, C.; Rogemond,
V.; Davoust, N.; Malcus, C.; Cavagna, S.; Gessain, A.; Machuca-Gayet,
I.; Belin, M.-F.; Quach, T.; Giraudon, P.: A role for the neuronal
protein collapsin response mediator protein 2 in T lymphocyte polarization
and migration. J. Immun. 175: 7650-7660, 2005.
6. Yoshimura, T.; Kawano, Y.; Arimura, N.; Kawabata, S.; Kikuchi,
A.; Kaibuchi, K.: GSK-3-beta regulates phosphorylation of CRMP-2
and neuronal polarity. Cell 120: 137-149, 2005.
*FIELD* CN
Cassandra L. Kniffin - updated: 8/3/2011
Paul J. Converse - updated: 8/4/2006
Stylianos E. Antonarakis - updated: 1/24/2005
Patricia A. Hartz - updated: 12/16/2002
Patti M. Sherman - updated: 4/23/1998
*FIELD* CD
Patti M. Sherman: 3/20/1998
*FIELD* ED
wwang: 08/05/2011
ckniffin: 8/3/2011
mgross: 8/30/2006
terry: 8/4/2006
mgross: 1/24/2005
mgross: 12/18/2002
terry: 12/16/2002
psherman: 4/23/1998
psherman: 3/24/1998
psherman: 3/23/1998
psherman: 3/20/1998