Full text data of LANCL2
LANCL2
(GPR69B, TASP)
[Confidence: low (only semi-automatic identification from reviews)]
LanC-like protein 2 (Testis-specific adriamycin sensitivity protein)
LanC-like protein 2 (Testis-specific adriamycin sensitivity protein)
UniProt
Q9NS86
ID LANC2_HUMAN Reviewed; 450 AA.
AC Q9NS86; B2R8D4; Q6NSL4; Q8TCQ3; Q9BSR1;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 103.
DE RecName: Full=LanC-like protein 2;
DE AltName: Full=Testis-specific adriamycin sensitivity protein;
GN Name=LANCL2; Synonyms=GPR69B, TASP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11762191;
RA Mayer H., Pongratz M., Prohaska R.;
RT "Molecular cloning, characterization, and tissue-specific expression
RT of human LANCL2, a novel member of the LanC-like protein family.";
RL DNA Seq. 12:161-166(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Sugimoto Y., Tsukahara S., Ishikawa E., Tsuruo T.;
RT "Isolation and identification of genes whose downregulation result in
RT anticancer drug resistance.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Eley G.D., Wang X.-Y., Frederick L., Hebrink D., Nash C., James C.D.;
RT "2 MB physical and transcript map of the 7p11.2 locus surrounding EGFR
RT frequently amplified in glioblastoma multiforme.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R.,
RA Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H.,
RA Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A.,
RA Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J.,
RA Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A.,
RA Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S.,
RA Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M.,
RA Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C.,
RA Latreille P., Miller N., Johnson D., Murray J., Woessner J.P.,
RA Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J.,
RA Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L.,
RA Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R.,
RA Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K.,
RA Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S.,
RA Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M.,
RA Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R.,
RA Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D.,
RA Waterston R.H., Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT PRO-56.
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S.,
RA Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z.,
RA Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C.,
RA Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J.,
RA Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F.,
RA Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F.,
RA Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H.,
RA Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G.,
RA Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P.,
RA Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J.,
RA Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F.,
RA Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B.,
RA Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W.,
RA Mural R.J., Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT PRO-56.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PRO-56.
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-450.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [10]
RP SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF GLY-2,
RP AND INTERACTION WITH INOSITOL PHOSPHOLIPIDS.
RX PubMed=16979580; DOI=10.1016/j.bbamem.2006.07.018;
RA Landlinger C., Salzer U., Prohaska R.;
RT "Myristoylation of human LanC-like protein 2 (LANCL2) is essential for
RT the interaction with the plasma membrane and the increase in cellular
RT sensitivity to adriamycin.";
RL Biochim. Biophys. Acta 1758:1759-1767(2006).
RN [11]
RP SUBCELLULAR LOCATION, AND POSSIBLE FUNCTION IN CELLULAR SENSITIVITY TO
RP ADRIAMYCIN.
RX PubMed=12566319;
RA Park S., James C.D.;
RT "Lanthionine synthetase components C-like 2 increases cellular
RT sensitivity to adriamycin by decreasing the expression of P-
RT glycoprotein through a transcription-mediated mechanism.";
RL Cancer Res. 63:723-727(2003).
RN [12]
RP FUNCTION.
RX PubMed=19667068; DOI=10.1074/jbc.M109.035329;
RA Sturla L., Fresia C., Guida L., Bruzzone S., Scarfi' S., Usai C.,
RA Fruscione F., Magnone M., Millo E., Basile G., Grozio A.,
RA Jacchetti E., Allegretti M., De Flora A., Zocchi E.;
RT "LANCL2 is necessary for abscisic acid binding and signaling in human
RT granulocytes and in rat insulinoma cells.";
RL J. Biol. Chem. 284:28045-28057(2009).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Necessary for abscisic acid (ABA) binding on the cell
CC membrane and activation of the ABA signaling pathway in
CC granulocytes.
CC -!- SUBUNIT: Interacts with an array of inositol phospholipids such as
CC phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-
CC phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). PIP-
CC binding enhances membrane association.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cell membrane.
CC Note=Localizes to the juxta-nuclear vesicles. Associates with the
CC cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-
CC cyclodextrin causes partial dissociation from the cell membrane in
CC vitro and an enhanced cell detachment from the matrix in vivo.
CC Membrane-association is important for the increased cellular
CC sensitivity to an anticancer drug (adriamycin).
CC -!- TISSUE SPECIFICITY: Expressed in brain and testis.
CC -!- PTM: Myristoylated. Essential for membrane association.
CC -!- MISCELLANEOUS: Its exogenous expression in a sarcoma cell line
CC decreases the expression of ABCB1 (P-glycoprotein 1) and increases
CC cellular sensitivity to an anticancer drug (adriamycin).
CC -!- SIMILARITY: Belongs to the LanC-like protein family.
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DR EMBL; AJ278245; CAC21715.1; -; mRNA.
DR EMBL; AB035966; BAA96800.1; -; mRNA.
DR EMBL; AF353942; AAK83286.1; -; mRNA.
DR EMBL; AK313326; BAG36131.1; -; mRNA.
DR EMBL; AC073347; AAQ93360.1; -; Genomic_DNA.
DR EMBL; CH236957; EAL23810.1; -; Genomic_DNA.
DR EMBL; CH471201; EAW50968.1; -; Genomic_DNA.
DR EMBL; BC004887; AAH04887.2; -; mRNA.
DR EMBL; BC070049; AAH70049.1; -; mRNA.
DR EMBL; AL713665; CAD28471.1; -; mRNA.
DR RefSeq; NP_061167.1; NM_018697.3.
DR UniGene; Hs.595384; -.
DR UniGene; Hs.706612; -.
DR ProteinModelPortal; Q9NS86; -.
DR SMR; Q9NS86; 19-444.
DR IntAct; Q9NS86; 2.
DR MINT; MINT-3073494; -.
DR STRING; 9606.ENSP00000254770; -.
DR PhosphoSite; Q9NS86; -.
DR DMDM; 47116933; -.
DR PaxDb; Q9NS86; -.
DR PRIDE; Q9NS86; -.
DR Ensembl; ENST00000254770; ENSP00000254770; ENSG00000132434.
DR GeneID; 55915; -.
DR KEGG; hsa:55915; -.
DR UCSC; uc003tqp.3; human.
DR CTD; 55915; -.
DR GeneCards; GC07P055400; -.
DR HGNC; HGNC:6509; LANCL2.
DR HPA; HPA019711; -.
DR HPA; HPA029535; -.
DR MIM; 612919; gene.
DR neXtProt; NX_Q9NS86; -.
DR PharmGKB; PA30294; -.
DR eggNOG; NOG245101; -.
DR HOGENOM; HOG000240926; -.
DR HOVERGEN; HBG065387; -.
DR InParanoid; Q9NS86; -.
DR OMA; NPFPDYE; -.
DR OrthoDB; EOG71VSTN; -.
DR PhylomeDB; Q9NS86; -.
DR ChiTaRS; LANCL2; human.
DR GeneWiki; LANCL2; -.
DR GenomeRNAi; 55915; -.
DR NextBio; 61311; -.
DR PRO; PR:Q9NS86; -.
DR ArrayExpress; Q9NS86; -.
DR Bgee; Q9NS86; -.
DR CleanEx; HS_LANCL2; -.
DR Genevestigator; Q9NS86; -.
DR GO; GO:0030864; C:cortical actin cytoskeleton; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; NAS:UniProtKB.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; NAS:UniProtKB.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:UniProtKB.
DR GO; GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:UniProtKB.
DR GO; GO:0010314; F:phosphatidylinositol-5-phosphate binding; IDA:UniProtKB.
DR GO; GO:0008152; P:metabolic process; IEA:GOC.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
DR GO; GO:0009789; P:positive regulation of abscisic acid mediated signaling pathway; IDA:UniProtKB.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR012341; 6hp_glycosidase.
DR InterPro; IPR007822; LANC-like.
DR InterPro; IPR020464; LanC-like_prot_euk.
DR Pfam; PF05147; LANC_like; 1.
DR PRINTS; PR01951; LANCEUKARYTE.
DR PRINTS; PR01950; LANCSUPER.
PE 1: Evidence at protein level;
KW Cell membrane; Complete proteome; Cytoplasm; Lipoprotein; Membrane;
KW Myristate; Nucleus; Phosphoprotein; Polymorphism; Reference proteome.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 450 LanC-like protein 2.
FT /FTId=PRO_0000191272.
FT REGION 2 15 Interaction with inositol phospholipids.
FT MOD_RES 198 198 Phosphotyrosine (By similarity).
FT LIPID 2 2 N-myristoyl glycine.
FT VARIANT 56 56 T -> P (in dbSNP:rs2272263).
FT /FTId=VAR_060064.
FT VARIANT 74 74 I -> V (in dbSNP:rs6961412).
FT /FTId=VAR_053480.
FT MUTAGEN 2 2 G->A: Loss of membrane localization and
FT results in localization to the nucleus,
FT particularly to the nucleoli.
SQ SEQUENCE 450 AA; 50854 MW; 8FD27E11BB91C05D CRC64;
MGETMSKRLK LHLGGEAEME ERAFVNPFPD YEAAAGALLA SGAAEETGCV RPPATTDEPG
LPFHQDGKII HNFIRRIQTK IKDLLQQMEE GLKTADPHDC SAYTGWTGIA LLYLQLYRVT
CDQTYLLRSL DYVKRTLRNL NGRRVTFLCG DAGPLAVGAV IYHKLRSDCE SQECVTKLLQ
LQRSVVCQES DLPDELLYGR AGYLYALLYL NTEIGPGTVC ESAIKEVVNA IIESGKTLSR
EERKTERCPL LYQWHRKQYV GAAHGMAGIY YMLMQPAAKV DQETLTEMVK PSIDYVRHKK
FRSGNYPSSL SNETDRLVHW CHGAPGVIHM LMQAYKVFKE EKYLKEAMEC SDVIWQRGLL
RKGYGICHGT AGNGYSFLSL YRLTQDKKYL YRACKFAEWC LDYGAHGCRI PDRPYSLFEG
MAGAIHFLSD VLGPETSRFP AFELDSSKRD
//
ID LANC2_HUMAN Reviewed; 450 AA.
AC Q9NS86; B2R8D4; Q6NSL4; Q8TCQ3; Q9BSR1;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2000, sequence version 1.
DT 22-JAN-2014, entry version 103.
DE RecName: Full=LanC-like protein 2;
DE AltName: Full=Testis-specific adriamycin sensitivity protein;
GN Name=LANCL2; Synonyms=GPR69B, TASP;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11762191;
RA Mayer H., Pongratz M., Prohaska R.;
RT "Molecular cloning, characterization, and tissue-specific expression
RT of human LANCL2, a novel member of the LanC-like protein family.";
RL DNA Seq. 12:161-166(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Sugimoto Y., Tsukahara S., Ishikawa E., Tsuruo T.;
RT "Isolation and identification of genes whose downregulation result in
RT anticancer drug resistance.";
RL Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Eley G.D., Wang X.-Y., Frederick L., Hebrink D., Nash C., James C.D.;
RT "2 MB physical and transcript map of the 7p11.2 locus surrounding EGFR
RT frequently amplified in glioblastoma multiforme.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R.,
RA Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H.,
RA Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A.,
RA Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J.,
RA Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A.,
RA Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S.,
RA Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M.,
RA Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C.,
RA Latreille P., Miller N., Johnson D., Murray J., Woessner J.P.,
RA Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J.,
RA Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L.,
RA Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R.,
RA Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K.,
RA Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S.,
RA Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M.,
RA Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R.,
RA Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D.,
RA Waterston R.H., Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT PRO-56.
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S.,
RA Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z.,
RA Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C.,
RA Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J.,
RA Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F.,
RA Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F.,
RA Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H.,
RA Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G.,
RA Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P.,
RA Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J.,
RA Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F.,
RA Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B.,
RA Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W.,
RA Mural R.J., Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT PRO-56.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PRO-56.
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-450.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H.,
RA Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K.,
RA Ottenwaelder B., Poustka A., Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [10]
RP SUBCELLULAR LOCATION, MYRISTOYLATION AT GLY-2, MUTAGENESIS OF GLY-2,
RP AND INTERACTION WITH INOSITOL PHOSPHOLIPIDS.
RX PubMed=16979580; DOI=10.1016/j.bbamem.2006.07.018;
RA Landlinger C., Salzer U., Prohaska R.;
RT "Myristoylation of human LanC-like protein 2 (LANCL2) is essential for
RT the interaction with the plasma membrane and the increase in cellular
RT sensitivity to adriamycin.";
RL Biochim. Biophys. Acta 1758:1759-1767(2006).
RN [11]
RP SUBCELLULAR LOCATION, AND POSSIBLE FUNCTION IN CELLULAR SENSITIVITY TO
RP ADRIAMYCIN.
RX PubMed=12566319;
RA Park S., James C.D.;
RT "Lanthionine synthetase components C-like 2 increases cellular
RT sensitivity to adriamycin by decreasing the expression of P-
RT glycoprotein through a transcription-mediated mechanism.";
RL Cancer Res. 63:723-727(2003).
RN [12]
RP FUNCTION.
RX PubMed=19667068; DOI=10.1074/jbc.M109.035329;
RA Sturla L., Fresia C., Guida L., Bruzzone S., Scarfi' S., Usai C.,
RA Fruscione F., Magnone M., Millo E., Basile G., Grozio A.,
RA Jacchetti E., Allegretti M., De Flora A., Zocchi E.;
RT "LANCL2 is necessary for abscisic acid binding and signaling in human
RT granulocytes and in rat insulinoma cells.";
RL J. Biol. Chem. 284:28045-28057(2009).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Necessary for abscisic acid (ABA) binding on the cell
CC membrane and activation of the ABA signaling pathway in
CC granulocytes.
CC -!- SUBUNIT: Interacts with an array of inositol phospholipids such as
CC phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4-
CC phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). PIP-
CC binding enhances membrane association.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cell membrane.
CC Note=Localizes to the juxta-nuclear vesicles. Associates with the
CC cortical actin cytoskeleton. Cholesterol depletion by methyl-beta-
CC cyclodextrin causes partial dissociation from the cell membrane in
CC vitro and an enhanced cell detachment from the matrix in vivo.
CC Membrane-association is important for the increased cellular
CC sensitivity to an anticancer drug (adriamycin).
CC -!- TISSUE SPECIFICITY: Expressed in brain and testis.
CC -!- PTM: Myristoylated. Essential for membrane association.
CC -!- MISCELLANEOUS: Its exogenous expression in a sarcoma cell line
CC decreases the expression of ABCB1 (P-glycoprotein 1) and increases
CC cellular sensitivity to an anticancer drug (adriamycin).
CC -!- SIMILARITY: Belongs to the LanC-like protein family.
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DR EMBL; AJ278245; CAC21715.1; -; mRNA.
DR EMBL; AB035966; BAA96800.1; -; mRNA.
DR EMBL; AF353942; AAK83286.1; -; mRNA.
DR EMBL; AK313326; BAG36131.1; -; mRNA.
DR EMBL; AC073347; AAQ93360.1; -; Genomic_DNA.
DR EMBL; CH236957; EAL23810.1; -; Genomic_DNA.
DR EMBL; CH471201; EAW50968.1; -; Genomic_DNA.
DR EMBL; BC004887; AAH04887.2; -; mRNA.
DR EMBL; BC070049; AAH70049.1; -; mRNA.
DR EMBL; AL713665; CAD28471.1; -; mRNA.
DR RefSeq; NP_061167.1; NM_018697.3.
DR UniGene; Hs.595384; -.
DR UniGene; Hs.706612; -.
DR ProteinModelPortal; Q9NS86; -.
DR SMR; Q9NS86; 19-444.
DR IntAct; Q9NS86; 2.
DR MINT; MINT-3073494; -.
DR STRING; 9606.ENSP00000254770; -.
DR PhosphoSite; Q9NS86; -.
DR DMDM; 47116933; -.
DR PaxDb; Q9NS86; -.
DR PRIDE; Q9NS86; -.
DR Ensembl; ENST00000254770; ENSP00000254770; ENSG00000132434.
DR GeneID; 55915; -.
DR KEGG; hsa:55915; -.
DR UCSC; uc003tqp.3; human.
DR CTD; 55915; -.
DR GeneCards; GC07P055400; -.
DR HGNC; HGNC:6509; LANCL2.
DR HPA; HPA019711; -.
DR HPA; HPA029535; -.
DR MIM; 612919; gene.
DR neXtProt; NX_Q9NS86; -.
DR PharmGKB; PA30294; -.
DR eggNOG; NOG245101; -.
DR HOGENOM; HOG000240926; -.
DR HOVERGEN; HBG065387; -.
DR InParanoid; Q9NS86; -.
DR OMA; NPFPDYE; -.
DR OrthoDB; EOG71VSTN; -.
DR PhylomeDB; Q9NS86; -.
DR ChiTaRS; LANCL2; human.
DR GeneWiki; LANCL2; -.
DR GenomeRNAi; 55915; -.
DR NextBio; 61311; -.
DR PRO; PR:Q9NS86; -.
DR ArrayExpress; Q9NS86; -.
DR Bgee; Q9NS86; -.
DR CleanEx; HS_LANCL2; -.
DR Genevestigator; Q9NS86; -.
DR GO; GO:0030864; C:cortical actin cytoskeleton; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; NAS:UniProtKB.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; NAS:UniProtKB.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:UniProtKB.
DR GO; GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:UniProtKB.
DR GO; GO:0010314; F:phosphatidylinositol-5-phosphate binding; IDA:UniProtKB.
DR GO; GO:0008152; P:metabolic process; IEA:GOC.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
DR GO; GO:0009789; P:positive regulation of abscisic acid mediated signaling pathway; IDA:UniProtKB.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR012341; 6hp_glycosidase.
DR InterPro; IPR007822; LANC-like.
DR InterPro; IPR020464; LanC-like_prot_euk.
DR Pfam; PF05147; LANC_like; 1.
DR PRINTS; PR01951; LANCEUKARYTE.
DR PRINTS; PR01950; LANCSUPER.
PE 1: Evidence at protein level;
KW Cell membrane; Complete proteome; Cytoplasm; Lipoprotein; Membrane;
KW Myristate; Nucleus; Phosphoprotein; Polymorphism; Reference proteome.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 450 LanC-like protein 2.
FT /FTId=PRO_0000191272.
FT REGION 2 15 Interaction with inositol phospholipids.
FT MOD_RES 198 198 Phosphotyrosine (By similarity).
FT LIPID 2 2 N-myristoyl glycine.
FT VARIANT 56 56 T -> P (in dbSNP:rs2272263).
FT /FTId=VAR_060064.
FT VARIANT 74 74 I -> V (in dbSNP:rs6961412).
FT /FTId=VAR_053480.
FT MUTAGEN 2 2 G->A: Loss of membrane localization and
FT results in localization to the nucleus,
FT particularly to the nucleoli.
SQ SEQUENCE 450 AA; 50854 MW; 8FD27E11BB91C05D CRC64;
MGETMSKRLK LHLGGEAEME ERAFVNPFPD YEAAAGALLA SGAAEETGCV RPPATTDEPG
LPFHQDGKII HNFIRRIQTK IKDLLQQMEE GLKTADPHDC SAYTGWTGIA LLYLQLYRVT
CDQTYLLRSL DYVKRTLRNL NGRRVTFLCG DAGPLAVGAV IYHKLRSDCE SQECVTKLLQ
LQRSVVCQES DLPDELLYGR AGYLYALLYL NTEIGPGTVC ESAIKEVVNA IIESGKTLSR
EERKTERCPL LYQWHRKQYV GAAHGMAGIY YMLMQPAAKV DQETLTEMVK PSIDYVRHKK
FRSGNYPSSL SNETDRLVHW CHGAPGVIHM LMQAYKVFKE EKYLKEAMEC SDVIWQRGLL
RKGYGICHGT AGNGYSFLSL YRLTQDKKYL YRACKFAEWC LDYGAHGCRI PDRPYSLFEG
MAGAIHFLSD VLGPETSRFP AFELDSSKRD
//
MIM
612919
*RECORD*
*FIELD* NO
612919
*FIELD* TI
*612919 LanC-LIKE 2; LANCL2
;;TESTIS-SPECIFIC ADRIAMYCIN SENSITIVITY PROTEIN; TASP
read more*FIELD* TX
CLONING
By searching for genes similar to LANCL1 (604155), followed by 3-prime
RACE of a human cerebral cortex cDNA library, Mayer et al. (2001) cloned
LANCL2. The deduced 450-amino acid protein contains a Walker A box and 7
repetitive hydrophobic regions that contain GxxG motifs. It shares 57.9%
amino acid identity with LANCL1. Northern blot analysis detected high
expression of 4.7- and 4.1-kb transcripts and weaker expression of 2.5-
and 1.8-kb transcripts in brain. The 1.8-kb transcript was highly
expressed in testis. All other tissues examined showed weak expression
of the 4.1- and 1.8-kb transcripts. RNA dot blot analysis confirmed
ubiquitous LANCL2 expression, with highest levels in amygdala, followed
by temporal lobe and testis. Database analysis revealed conservation of
LANCL proteins in bacteria, plant, and animal genomes.
Using Western blot and immunohistochemical analyses, Park and James
(2003) found that epitope-tagged LANCL2 distributed to the plasma
membrane, cytosol, and nucleus of a transfected human uterine sarcoma
cell line.
GENE FUNCTION
Using a human uterine sarcoma cell line and a multidrug-resistant
derivative, Park and James (2003) found that ectopic expression of
LANCL2 significantly reduced expression of the multidrug resistance gene
MDR1 (ABCB1; 171050) and increased cell sensitivity toward the
chemotherapeutic agent adriamycin. Reporter gene assays revealed that
LANCL2 repressed transcription of the MDR1 promoter.
Zhang et al. (2009) showed that LANCL2 exhibited Zn(2+)-dependent
glutathione binding. LANCL2 bound the SH3 domain of mouse Eps8 (600206),
but not other SH3-containing proteins. The interaction between LANCL2
and Eps8 did not require either of the PxxP motifs of LANCL2, but it was
inhibited by glutathione.
MAPPING
Park and James (2003) stated that the LANCL2 gene maps within 200 bp of
the EGFR gene (131550) on chromosome 7p11.2.
*FIELD* RF
1. Mayer, H.; Pongratz, M.; Prohaska, R.: Molecular cloning, characterization,
and tissue-specific expression of human LANCL2, a novel member of
the LanC-like protein family. DNA Seq. 12: 161-166, 2001.
2. Park, S.; James, C. D.: Lanthionine synthetase components C-like
2 increases cellular sensitivity to adriamycin by decreasing the expression
of P-glycoprotein through a transcription-mediated mechanism. Cancer
Res. 63: 723-727, 2003.
3. Zhang, W.; Wang, L.; Liu, Y.; Xu, J.; Zhu, G.; Cang, H.; Li, X.;
Bartlam, M.; Hensley, K.; Li, G.; Rao, Z.; Zhang, X. C.: Structure
of human lanthionine synthetase C-like protein 1 and its interaction
with Eps8 and glutathione. Genes Dev. 23: 1387-1392, 2009.
*FIELD* CD
Patricia A. Hartz: 7/17/2009
*FIELD* ED
mgross: 07/17/2009
*RECORD*
*FIELD* NO
612919
*FIELD* TI
*612919 LanC-LIKE 2; LANCL2
;;TESTIS-SPECIFIC ADRIAMYCIN SENSITIVITY PROTEIN; TASP
read more*FIELD* TX
CLONING
By searching for genes similar to LANCL1 (604155), followed by 3-prime
RACE of a human cerebral cortex cDNA library, Mayer et al. (2001) cloned
LANCL2. The deduced 450-amino acid protein contains a Walker A box and 7
repetitive hydrophobic regions that contain GxxG motifs. It shares 57.9%
amino acid identity with LANCL1. Northern blot analysis detected high
expression of 4.7- and 4.1-kb transcripts and weaker expression of 2.5-
and 1.8-kb transcripts in brain. The 1.8-kb transcript was highly
expressed in testis. All other tissues examined showed weak expression
of the 4.1- and 1.8-kb transcripts. RNA dot blot analysis confirmed
ubiquitous LANCL2 expression, with highest levels in amygdala, followed
by temporal lobe and testis. Database analysis revealed conservation of
LANCL proteins in bacteria, plant, and animal genomes.
Using Western blot and immunohistochemical analyses, Park and James
(2003) found that epitope-tagged LANCL2 distributed to the plasma
membrane, cytosol, and nucleus of a transfected human uterine sarcoma
cell line.
GENE FUNCTION
Using a human uterine sarcoma cell line and a multidrug-resistant
derivative, Park and James (2003) found that ectopic expression of
LANCL2 significantly reduced expression of the multidrug resistance gene
MDR1 (ABCB1; 171050) and increased cell sensitivity toward the
chemotherapeutic agent adriamycin. Reporter gene assays revealed that
LANCL2 repressed transcription of the MDR1 promoter.
Zhang et al. (2009) showed that LANCL2 exhibited Zn(2+)-dependent
glutathione binding. LANCL2 bound the SH3 domain of mouse Eps8 (600206),
but not other SH3-containing proteins. The interaction between LANCL2
and Eps8 did not require either of the PxxP motifs of LANCL2, but it was
inhibited by glutathione.
MAPPING
Park and James (2003) stated that the LANCL2 gene maps within 200 bp of
the EGFR gene (131550) on chromosome 7p11.2.
*FIELD* RF
1. Mayer, H.; Pongratz, M.; Prohaska, R.: Molecular cloning, characterization,
and tissue-specific expression of human LANCL2, a novel member of
the LanC-like protein family. DNA Seq. 12: 161-166, 2001.
2. Park, S.; James, C. D.: Lanthionine synthetase components C-like
2 increases cellular sensitivity to adriamycin by decreasing the expression
of P-glycoprotein through a transcription-mediated mechanism. Cancer
Res. 63: 723-727, 2003.
3. Zhang, W.; Wang, L.; Liu, Y.; Xu, J.; Zhu, G.; Cang, H.; Li, X.;
Bartlam, M.; Hensley, K.; Li, G.; Rao, Z.; Zhang, X. C.: Structure
of human lanthionine synthetase C-like protein 1 and its interaction
with Eps8 and glutathione. Genes Dev. 23: 1387-1392, 2009.
*FIELD* CD
Patricia A. Hartz: 7/17/2009
*FIELD* ED
mgross: 07/17/2009