Full text data of SLC43A1
SLC43A1
(LAT3, PB39, POV1)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Large neutral amino acids transporter small subunit 3 (L-type amino acid transporter 3; Prostate cancer overexpressed gene 1 protein; Solute carrier family 43 member 1)
Large neutral amino acids transporter small subunit 3 (L-type amino acid transporter 3; Prostate cancer overexpressed gene 1 protein; Solute carrier family 43 member 1)
hRBCD
IPI00021075
IPI00021075 PB39 PB39 membrane n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 1 n/a n/a 1 n/a n/a n/a n/a integral membrane protein n/a found at its expected molecular weight found at molecular weight
IPI00021075 PB39 PB39 membrane n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a 1 n/a n/a 1 n/a n/a n/a n/a integral membrane protein n/a found at its expected molecular weight found at molecular weight
UniProt
O75387
ID LAT3_HUMAN Reviewed; 559 AA.
AC O75387;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1998, sequence version 1.
DT 22-JAN-2014, entry version 97.
DE RecName: Full=Large neutral amino acids transporter small subunit 3;
DE AltName: Full=L-type amino acid transporter 3;
DE AltName: Full=Prostate cancer overexpressed gene 1 protein;
DE AltName: Full=Solute carrier family 43 member 1;
GN Name=SLC43A1; Synonyms=LAT3, PB39, POV1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=9722952; DOI=10.1006/geno.1998.5359;
RA Cole K.A., Chuaqui R.F., Katz K., Pack S., Zhuang Z., Cole C.E.,
RA Lyne J.C., Linehan W.M., Liotta L.A., Emmert-Buck M.R.;
RT "cDNA sequencing and analysis of POV1 (PB39): a novel gene up-
RT regulated in prostate cancer.";
RL Genomics 51:282-287(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Liver;
RX PubMed=12930836; DOI=10.1074/jbc.M305221200;
RA Babu E., Kanai Y., Chairoungdua A., Kim D.K., Iribe Y.,
RA Tangtrongsup S., Jutabha P., Li Y., Ahmed N., Sakamoto S., Anzai N.,
RA Nagamori S., Endou H.;
RT "Identification of a novel system L amino acid transporter
RT structurally distinct from heterodimeric amino acid transporters.";
RL J. Biol. Chem. 278:43838-43845(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM 1), FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=9255310; DOI=10.1016/S0090-4295(97)00194-5;
RA Chuaqui R.F., Englert C.R., Strup S.E., Vocke C.D., Zhuang Z.,
RA Duray P.H., Bostwick D.G., Linehan W.M., Liotta L.A.,
RA Emmert-Buck M.R.;
RT "Identification of a novel transcript up-regulated in a clinically
RT aggressive prostate carcinoma.";
RL Urology 50:302-307(1997).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11956097;
RA Skotheim R.I., Monni O., Mousses S., Fossaa S.D., Kallioniemi O.-P.,
RA Lothe R.A., Kallioniemi A.;
RT "New insights into testicular germ cell tumorigenesis from gene
RT expression profiling.";
RL Cancer Res. 62:2359-2364(2002).
CC -!- FUNCTION: Sodium-independent, high affinity transport of large
CC neutral amino acids. Has narrower substrate selectivity compared
CC to SLC7A5 and SLC7A8 and mainly transports branched-chain amino
CC acids and phenylalanine. Plays a role in the development of human
CC prostate cancer, from prostatic intraepithelial neoplasia to
CC invasive prostate cancer.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC (Probable).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=2.3kb;
CC IsoId=O75387-1; Sequence=Displayed;
CC Name=2; Synonyms=5kb;
CC IsoId=O75387-2; Sequence=VSP_051620;
CC -!- TISSUE SPECIFICITY: In adults, found in all tissues examined with
CC highest expression in pancreas. In fetus, highest expression in
CC liver and lower levels in kidney, and lung. High levels found in
CC prostate cancer cells.
CC -!- MISCELLANEOUS: Up-regulated in early prostate cancer development
CC with highest expression level in seminomas of testicular germ cell
CC tumors.
CC -!- SIMILARITY: Belongs to the SLC43A transporter (TC 2.A.1.44)
CC family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF045584; AAC33004.1; -; mRNA.
DR EMBL; AB103033; BAD00152.1; -; mRNA.
DR EMBL; BC001639; AAH01639.1; -; mRNA.
DR RefSeq; NP_001185739.1; NM_001198810.1.
DR RefSeq; NP_003618.1; NM_003627.5.
DR UniGene; Hs.591952; -.
DR UniGene; Hs.735724; -.
DR ProteinModelPortal; O75387; -.
DR DIP; DIP-47272N; -.
DR STRING; 9606.ENSP00000278426; -.
DR BindingDB; O75387; -.
DR TCDB; 2.A.1.44.1; the major facilitator superfamily (mfs).
DR PhosphoSite; O75387; -.
DR PaxDb; O75387; -.
DR PRIDE; O75387; -.
DR Ensembl; ENST00000278426; ENSP00000278426; ENSG00000149150.
DR Ensembl; ENST00000528450; ENSP00000435673; ENSG00000149150.
DR GeneID; 8501; -.
DR KEGG; hsa:8501; -.
DR UCSC; uc001nkk.3; human.
DR CTD; 8501; -.
DR GeneCards; GC11M057252; -.
DR HGNC; HGNC:9225; SLC43A1.
DR HPA; HPA018813; -.
DR HPA; HPA018826; -.
DR MIM; 603733; gene.
DR neXtProt; NX_O75387; -.
DR PharmGKB; PA33549; -.
DR eggNOG; NOG71084; -.
DR HOGENOM; HOG000231937; -.
DR HOVERGEN; HBG072879; -.
DR InParanoid; O75387; -.
DR KO; K08228; -.
DR OMA; NWPVEAF; -.
DR OrthoDB; EOG712TWX; -.
DR PhylomeDB; O75387; -.
DR Reactome; REACT_15518; Transmembrane transport of small molecules.
DR Reactome; REACT_19419; Amino acid and oligopeptide SLC transporters.
DR ChiTaRS; SLC43A1; human.
DR GeneWiki; SLC43A1; -.
DR GenomeRNAi; 8501; -.
DR NextBio; 31811; -.
DR PRO; PR:O75387; -.
DR ArrayExpress; O75387; -.
DR Bgee; O75387; -.
DR CleanEx; HS_SLC43A1; -.
DR Genevestigator; O75387; -.
DR GO; GO:0005887; C:integral to plasma membrane; TAS:UniProtKB.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; IDA:UniProtKB.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR016196; MFS_dom_general_subst_transpt.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid transport; Complete proteome;
KW Glycoprotein; Membrane; Polymorphism; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 559 Large neutral amino acids transporter
FT small subunit 3.
FT /FTId=PRO_0000218640.
FT TRANSMEM 20 40 Helical; (Potential).
FT TRANSMEM 78 98 Helical; (Potential).
FT TRANSMEM 105 124 Helical; (Potential).
FT TRANSMEM 131 151 Helical; (Potential).
FT TRANSMEM 168 188 Helical; (Potential).
FT TRANSMEM 191 211 Helical; (Potential).
FT TRANSMEM 304 324 Helical; (Potential).
FT TRANSMEM 357 377 Helical; (Potential).
FT TRANSMEM 419 439 Helical; (Potential).
FT TRANSMEM 446 466 Helical; (Potential).
FT TRANSMEM 485 505 Helical; (Potential).
FT TRANSMEM 510 530 Helical; (Potential).
FT CARBOHYD 57 57 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 212 212 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 229 229 N-linked (GlcNAc...) (Potential).
FT VAR_SEQ 513 559 NLGLLLFSLLGFLLPSYLFYYRARLQQEYAANGMGPLKVLS
FT GSEVTA -> RARVGVGGAGATLLGAGVGPCMWCHPSLISA
FT RGTSEVSNLQVSKLSAF (in isoform 2).
FT /FTId=VSP_051620.
FT VARIANT 238 238 G -> V (in dbSNP:rs17151933).
FT /FTId=VAR_053670.
FT VARIANT 443 443 H -> Y (in dbSNP:rs34746107).
FT /FTId=VAR_053671.
SQ SEQUENCE 559 AA; 61477 MW; 49B0DECA3D930A45 CRC64;
MAPTLQQAYR RRWWMACTAV LENLFFSAVL LGWGSLLIIL KNEGFYSSTC PAESSTNTTQ
DEQRRWPGCD QQDEMLNLGF TIGSFVLSAT TLPLGILMDR FGPRPVRLVG SACFTASCTL
MALASRDVEA LSPLIFLALS LNGFGGICLT FTSLTLPNMF GNLRSTLMAL MIGSYASSAI
TFPGIKLIYD AGVAFVVIMF TWSGLACLIF LNCTLNWPIE AFPAPEEVNY TKKIKLSGLA
LDHKVTGDLF YTHVTTMGQR LSQKAPSLED GSDAFMSPQD VRGTSENLPE RSVPLRKSLC
SPTFLWSLLT MGMTQLRIIF YMAAVNKMLE YLVTGGQEHE TNEQQQKVAE TVGFYSSVFG
AMQLLCLLTC PLIGYIMDWR IKDCVDAPTQ GTVLGDARDG VATKSIRPRY CKIQKLTNAI
SAFTLTNLLL VGFGITCLIN NLHLQFVTFV LHTIVRGFFH SACGSLYAAV FPSNHFGTLT
GLQSLISAVF ALLQQPLFMA MVGPLKGEPF WVNLGLLLFS LLGFLLPSYL FYYRARLQQE
YAANGMGPLK VLSGSEVTA
//
ID LAT3_HUMAN Reviewed; 559 AA.
AC O75387;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-NOV-1998, sequence version 1.
DT 22-JAN-2014, entry version 97.
DE RecName: Full=Large neutral amino acids transporter small subunit 3;
DE AltName: Full=L-type amino acid transporter 3;
DE AltName: Full=Prostate cancer overexpressed gene 1 protein;
DE AltName: Full=Solute carrier family 43 member 1;
GN Name=SLC43A1; Synonyms=LAT3, PB39, POV1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=9722952; DOI=10.1006/geno.1998.5359;
RA Cole K.A., Chuaqui R.F., Katz K., Pack S., Zhuang Z., Cole C.E.,
RA Lyne J.C., Linehan W.M., Liotta L.A., Emmert-Buck M.R.;
RT "cDNA sequencing and analysis of POV1 (PB39): a novel gene up-
RT regulated in prostate cancer.";
RL Genomics 51:282-287(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Liver;
RX PubMed=12930836; DOI=10.1074/jbc.M305221200;
RA Babu E., Kanai Y., Chairoungdua A., Kim D.K., Iribe Y.,
RA Tangtrongsup S., Jutabha P., Li Y., Ahmed N., Sakamoto S., Anzai N.,
RA Nagamori S., Endou H.;
RT "Identification of a novel system L amino acid transporter
RT structurally distinct from heterodimeric amino acid transporters.";
RL J. Biol. Chem. 278:43838-43845(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM 1), FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=9255310; DOI=10.1016/S0090-4295(97)00194-5;
RA Chuaqui R.F., Englert C.R., Strup S.E., Vocke C.D., Zhuang Z.,
RA Duray P.H., Bostwick D.G., Linehan W.M., Liotta L.A.,
RA Emmert-Buck M.R.;
RT "Identification of a novel transcript up-regulated in a clinically
RT aggressive prostate carcinoma.";
RL Urology 50:302-307(1997).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11956097;
RA Skotheim R.I., Monni O., Mousses S., Fossaa S.D., Kallioniemi O.-P.,
RA Lothe R.A., Kallioniemi A.;
RT "New insights into testicular germ cell tumorigenesis from gene
RT expression profiling.";
RL Cancer Res. 62:2359-2364(2002).
CC -!- FUNCTION: Sodium-independent, high affinity transport of large
CC neutral amino acids. Has narrower substrate selectivity compared
CC to SLC7A5 and SLC7A8 and mainly transports branched-chain amino
CC acids and phenylalanine. Plays a role in the development of human
CC prostate cancer, from prostatic intraepithelial neoplasia to
CC invasive prostate cancer.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC (Probable).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=2.3kb;
CC IsoId=O75387-1; Sequence=Displayed;
CC Name=2; Synonyms=5kb;
CC IsoId=O75387-2; Sequence=VSP_051620;
CC -!- TISSUE SPECIFICITY: In adults, found in all tissues examined with
CC highest expression in pancreas. In fetus, highest expression in
CC liver and lower levels in kidney, and lung. High levels found in
CC prostate cancer cells.
CC -!- MISCELLANEOUS: Up-regulated in early prostate cancer development
CC with highest expression level in seminomas of testicular germ cell
CC tumors.
CC -!- SIMILARITY: Belongs to the SLC43A transporter (TC 2.A.1.44)
CC family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF045584; AAC33004.1; -; mRNA.
DR EMBL; AB103033; BAD00152.1; -; mRNA.
DR EMBL; BC001639; AAH01639.1; -; mRNA.
DR RefSeq; NP_001185739.1; NM_001198810.1.
DR RefSeq; NP_003618.1; NM_003627.5.
DR UniGene; Hs.591952; -.
DR UniGene; Hs.735724; -.
DR ProteinModelPortal; O75387; -.
DR DIP; DIP-47272N; -.
DR STRING; 9606.ENSP00000278426; -.
DR BindingDB; O75387; -.
DR TCDB; 2.A.1.44.1; the major facilitator superfamily (mfs).
DR PhosphoSite; O75387; -.
DR PaxDb; O75387; -.
DR PRIDE; O75387; -.
DR Ensembl; ENST00000278426; ENSP00000278426; ENSG00000149150.
DR Ensembl; ENST00000528450; ENSP00000435673; ENSG00000149150.
DR GeneID; 8501; -.
DR KEGG; hsa:8501; -.
DR UCSC; uc001nkk.3; human.
DR CTD; 8501; -.
DR GeneCards; GC11M057252; -.
DR HGNC; HGNC:9225; SLC43A1.
DR HPA; HPA018813; -.
DR HPA; HPA018826; -.
DR MIM; 603733; gene.
DR neXtProt; NX_O75387; -.
DR PharmGKB; PA33549; -.
DR eggNOG; NOG71084; -.
DR HOGENOM; HOG000231937; -.
DR HOVERGEN; HBG072879; -.
DR InParanoid; O75387; -.
DR KO; K08228; -.
DR OMA; NWPVEAF; -.
DR OrthoDB; EOG712TWX; -.
DR PhylomeDB; O75387; -.
DR Reactome; REACT_15518; Transmembrane transport of small molecules.
DR Reactome; REACT_19419; Amino acid and oligopeptide SLC transporters.
DR ChiTaRS; SLC43A1; human.
DR GeneWiki; SLC43A1; -.
DR GenomeRNAi; 8501; -.
DR NextBio; 31811; -.
DR PRO; PR:O75387; -.
DR ArrayExpress; O75387; -.
DR Bgee; O75387; -.
DR CleanEx; HS_SLC43A1; -.
DR Genevestigator; O75387; -.
DR GO; GO:0005887; C:integral to plasma membrane; TAS:UniProtKB.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; IDA:UniProtKB.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR016196; MFS_dom_general_subst_transpt.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 3.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid transport; Complete proteome;
KW Glycoprotein; Membrane; Polymorphism; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 559 Large neutral amino acids transporter
FT small subunit 3.
FT /FTId=PRO_0000218640.
FT TRANSMEM 20 40 Helical; (Potential).
FT TRANSMEM 78 98 Helical; (Potential).
FT TRANSMEM 105 124 Helical; (Potential).
FT TRANSMEM 131 151 Helical; (Potential).
FT TRANSMEM 168 188 Helical; (Potential).
FT TRANSMEM 191 211 Helical; (Potential).
FT TRANSMEM 304 324 Helical; (Potential).
FT TRANSMEM 357 377 Helical; (Potential).
FT TRANSMEM 419 439 Helical; (Potential).
FT TRANSMEM 446 466 Helical; (Potential).
FT TRANSMEM 485 505 Helical; (Potential).
FT TRANSMEM 510 530 Helical; (Potential).
FT CARBOHYD 57 57 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 212 212 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 229 229 N-linked (GlcNAc...) (Potential).
FT VAR_SEQ 513 559 NLGLLLFSLLGFLLPSYLFYYRARLQQEYAANGMGPLKVLS
FT GSEVTA -> RARVGVGGAGATLLGAGVGPCMWCHPSLISA
FT RGTSEVSNLQVSKLSAF (in isoform 2).
FT /FTId=VSP_051620.
FT VARIANT 238 238 G -> V (in dbSNP:rs17151933).
FT /FTId=VAR_053670.
FT VARIANT 443 443 H -> Y (in dbSNP:rs34746107).
FT /FTId=VAR_053671.
SQ SEQUENCE 559 AA; 61477 MW; 49B0DECA3D930A45 CRC64;
MAPTLQQAYR RRWWMACTAV LENLFFSAVL LGWGSLLIIL KNEGFYSSTC PAESSTNTTQ
DEQRRWPGCD QQDEMLNLGF TIGSFVLSAT TLPLGILMDR FGPRPVRLVG SACFTASCTL
MALASRDVEA LSPLIFLALS LNGFGGICLT FTSLTLPNMF GNLRSTLMAL MIGSYASSAI
TFPGIKLIYD AGVAFVVIMF TWSGLACLIF LNCTLNWPIE AFPAPEEVNY TKKIKLSGLA
LDHKVTGDLF YTHVTTMGQR LSQKAPSLED GSDAFMSPQD VRGTSENLPE RSVPLRKSLC
SPTFLWSLLT MGMTQLRIIF YMAAVNKMLE YLVTGGQEHE TNEQQQKVAE TVGFYSSVFG
AMQLLCLLTC PLIGYIMDWR IKDCVDAPTQ GTVLGDARDG VATKSIRPRY CKIQKLTNAI
SAFTLTNLLL VGFGITCLIN NLHLQFVTFV LHTIVRGFFH SACGSLYAAV FPSNHFGTLT
GLQSLISAVF ALLQQPLFMA MVGPLKGEPF WVNLGLLLFS LLGFLLPSYL FYYRARLQQE
YAANGMGPLK VLSGSEVTA
//
MIM
603733
*RECORD*
*FIELD* NO
603733
*FIELD* TI
*603733 SOLUTE CARRIER FAMILY 43 (L-TYPE AMINO ACID TRANSPORTER), MEMBER 1;
SLC43A1
read more;;L-TYPE AMINO ACID TRANSPORTER 3; LAT3;;
PROSTATE CANCER OVEREXPRESSED GENE 1; POV1
*FIELD* TX
DESCRIPTION
SLC43A1 belongs to the system L family of plasma membrane carrier
proteins that transports large neutral amino acids (Babu et al., 2003).
CLONING
To assess prostate cancer-associated gene expression in vivo, Chuaqui et
al. (1997) performed RT-PCR-differential display, using zinc
finger-directed primers, on RNA extracted from microdissected normal
prostatic epithelium and the corresponding invasive prostatic tumor. The
authors cloned POV1, which they called R00504, as a cDNA that appeared
selectively in the tumor samples. Northern blot analysis detected a
2.6-kb POV1 transcript in a variety of fetal tissues, with the highest
level in fetal liver.
Cole et al. (1998) cloned a full-length cDNA corresponding to POV1,
which they called PB39. The deduced 559-amino acid POV1 protein contains
a putative secretory signal peptide and a leucine zipper motif. Northern
blot analysis of adult tissues detected highest POV1 expression in
pancreas. Analysis of POV1 expression in human tissues by EST database
searching and by RT-PCR analysis demonstrated the presence of a unique
POV1 splice variant mRNA that is primarily associated with fetal tissues
and tumors.
By screening a human hepatocarcinoma cell line cDNA library for cDNAs
that increased the uptake of radioactive leucine into Xenopus oocytes,
Babu et al. (2003) cloned SLC43A1, which they called LAT3. The deduced
559-amino acid protein contains 12 transmembrane domains and a leucine
zipper motif. A long intracellular loop between transmembrane domains 1
and 2 contains 2 N-glycosylation sites, and 2 other intracellular loops
contain putative phosphorylation sites. Northern blot analysis of human
tissues detected a 2.5-kb transcript highly expressed in pancreas,
liver, skeletal muscle, and fetal liver. Expression was weaker in heart,
placenta, lung, kidney, spleen, prostate, testis, ovary, small
intestine, colon, lymph node, and bone marrow, and no expression was
detected in other tissues examined. Pancreas contained an additional
message of 4.4 kb.
GENE FUNCTION
Chuaqui et al. (1997) found that prostate carcinoma samples from 5 of 10
patients showed substantial overexpression of POV1. Although the
clinical parameters of the tumors varied, they found no correlation
between POV1 expression and the clinical features of the tumors.
Babu et al. (2003) found that LAT3 increased the transport of neutral
amino acids such as L-leucine, L-isoleucine, L-valine, and
L-phenylalanine following expression in Xenopus oocytes. Transport was
concentration-dependent, Na(+)-independent, and inhibited by a system L
transport inhibitor. In contrast to the other system L transporters,
LAT1 (SLC7A5; 600182) and LAT2 (SLC7A8; 604235), LAT3 transport activity
did not require formation of a heterodimeric complex with 4F2 heavy
chain (SLC3A2; 158070). In addition to amino acid substrates, LAT3
recognized amino acid alcohols. The transport of L-leucine was
electroneutral and mediated by a facilitated diffusion. In contrast,
L-leucinol, L-valinol, and L-phenylalinol, which have net positive
charge, induced inward currents under voltage clamp. LAT3-mediated
transport was inhibited by pretreatment with the thiol reagent
N-ethylmaleimide.
MAPPING
Cole et al. (1998) mapped the SLC43A1 gene to chromosome 11p11.2-p11.1
by fluorescence in situ hybridization.
*FIELD* RF
1. Babu, E.; Kanai, Y.; Chairoungdua, A.; Kim, D. K.; Iribe, Y.; Tangtrongsup,
S.; Jutabha, P.; Li, Y.; Ahmed, N.; Sakamoto, S.; Anzai, N.; Nagamori,
S.; Endou, H.: Identification of a novel system L amino acid transporter
structurally distinct from heterodimeric amino acid transporters. J.
Biol. Chem. 278: 43838-43845, 2003.
2. Chuaqui, R. F.; Englert, C. R.; Strup, S. E.; Vocke, C. D.; Zhuang,
Z.; Duray, P. H.; Bostwick, D. G.; Linehan, W. M.; Liotta, L. A.;
Emmert-Buck, M. R.: Identification of a novel transcript up-regulated
in a clinically aggressive prostate carcinoma. Urology 50: 302-307,
1997.
3. Cole, K. A.; Chuaqui, R. F.; Katz, K.; Pack, S.; Zhuang, Z.; Cole,
C. E.; Lyne, J. C.; Linehan, W. M.; Liotta, L. A.; Emmert-Buck, M.
R.: cDNA sequencing and analysis of POV1 (PB39): a novel gene up-regulated
in prostate cancer. Genomics 51: 282-287, 1998.
*FIELD* CN
Patricia A. Hartz - updated: 3/19/2007
*FIELD* CD
Sheryl A. Jankowski: 4/15/1999
*FIELD* ED
wwang: 04/09/2007
terry: 3/19/2007
mgross: 2/22/2007
tkritzer: 2/20/2003
psherman: 4/15/1999
*RECORD*
*FIELD* NO
603733
*FIELD* TI
*603733 SOLUTE CARRIER FAMILY 43 (L-TYPE AMINO ACID TRANSPORTER), MEMBER 1;
SLC43A1
read more;;L-TYPE AMINO ACID TRANSPORTER 3; LAT3;;
PROSTATE CANCER OVEREXPRESSED GENE 1; POV1
*FIELD* TX
DESCRIPTION
SLC43A1 belongs to the system L family of plasma membrane carrier
proteins that transports large neutral amino acids (Babu et al., 2003).
CLONING
To assess prostate cancer-associated gene expression in vivo, Chuaqui et
al. (1997) performed RT-PCR-differential display, using zinc
finger-directed primers, on RNA extracted from microdissected normal
prostatic epithelium and the corresponding invasive prostatic tumor. The
authors cloned POV1, which they called R00504, as a cDNA that appeared
selectively in the tumor samples. Northern blot analysis detected a
2.6-kb POV1 transcript in a variety of fetal tissues, with the highest
level in fetal liver.
Cole et al. (1998) cloned a full-length cDNA corresponding to POV1,
which they called PB39. The deduced 559-amino acid POV1 protein contains
a putative secretory signal peptide and a leucine zipper motif. Northern
blot analysis of adult tissues detected highest POV1 expression in
pancreas. Analysis of POV1 expression in human tissues by EST database
searching and by RT-PCR analysis demonstrated the presence of a unique
POV1 splice variant mRNA that is primarily associated with fetal tissues
and tumors.
By screening a human hepatocarcinoma cell line cDNA library for cDNAs
that increased the uptake of radioactive leucine into Xenopus oocytes,
Babu et al. (2003) cloned SLC43A1, which they called LAT3. The deduced
559-amino acid protein contains 12 transmembrane domains and a leucine
zipper motif. A long intracellular loop between transmembrane domains 1
and 2 contains 2 N-glycosylation sites, and 2 other intracellular loops
contain putative phosphorylation sites. Northern blot analysis of human
tissues detected a 2.5-kb transcript highly expressed in pancreas,
liver, skeletal muscle, and fetal liver. Expression was weaker in heart,
placenta, lung, kidney, spleen, prostate, testis, ovary, small
intestine, colon, lymph node, and bone marrow, and no expression was
detected in other tissues examined. Pancreas contained an additional
message of 4.4 kb.
GENE FUNCTION
Chuaqui et al. (1997) found that prostate carcinoma samples from 5 of 10
patients showed substantial overexpression of POV1. Although the
clinical parameters of the tumors varied, they found no correlation
between POV1 expression and the clinical features of the tumors.
Babu et al. (2003) found that LAT3 increased the transport of neutral
amino acids such as L-leucine, L-isoleucine, L-valine, and
L-phenylalanine following expression in Xenopus oocytes. Transport was
concentration-dependent, Na(+)-independent, and inhibited by a system L
transport inhibitor. In contrast to the other system L transporters,
LAT1 (SLC7A5; 600182) and LAT2 (SLC7A8; 604235), LAT3 transport activity
did not require formation of a heterodimeric complex with 4F2 heavy
chain (SLC3A2; 158070). In addition to amino acid substrates, LAT3
recognized amino acid alcohols. The transport of L-leucine was
electroneutral and mediated by a facilitated diffusion. In contrast,
L-leucinol, L-valinol, and L-phenylalinol, which have net positive
charge, induced inward currents under voltage clamp. LAT3-mediated
transport was inhibited by pretreatment with the thiol reagent
N-ethylmaleimide.
MAPPING
Cole et al. (1998) mapped the SLC43A1 gene to chromosome 11p11.2-p11.1
by fluorescence in situ hybridization.
*FIELD* RF
1. Babu, E.; Kanai, Y.; Chairoungdua, A.; Kim, D. K.; Iribe, Y.; Tangtrongsup,
S.; Jutabha, P.; Li, Y.; Ahmed, N.; Sakamoto, S.; Anzai, N.; Nagamori,
S.; Endou, H.: Identification of a novel system L amino acid transporter
structurally distinct from heterodimeric amino acid transporters. J.
Biol. Chem. 278: 43838-43845, 2003.
2. Chuaqui, R. F.; Englert, C. R.; Strup, S. E.; Vocke, C. D.; Zhuang,
Z.; Duray, P. H.; Bostwick, D. G.; Linehan, W. M.; Liotta, L. A.;
Emmert-Buck, M. R.: Identification of a novel transcript up-regulated
in a clinically aggressive prostate carcinoma. Urology 50: 302-307,
1997.
3. Cole, K. A.; Chuaqui, R. F.; Katz, K.; Pack, S.; Zhuang, Z.; Cole,
C. E.; Lyne, J. C.; Linehan, W. M.; Liotta, L. A.; Emmert-Buck, M.
R.: cDNA sequencing and analysis of POV1 (PB39): a novel gene up-regulated
in prostate cancer. Genomics 51: 282-287, 1998.
*FIELD* CN
Patricia A. Hartz - updated: 3/19/2007
*FIELD* CD
Sheryl A. Jankowski: 4/15/1999
*FIELD* ED
wwang: 04/09/2007
terry: 3/19/2007
mgross: 2/22/2007
tkritzer: 2/20/2003
psherman: 4/15/1999