Full text data of SLC43A2
SLC43A2
(LAT4)
[Confidence: medium (present in either hRBCD or BSc_CH or PM22954596)]
Large neutral amino acids transporter small subunit 4 (L-type amino acid transporter 4; Solute carrier family 43 member 2)
Large neutral amino acids transporter small subunit 4 (L-type amino acid transporter 4; Solute carrier family 43 member 2)
hRBCD
IPI00171004
IPI00171004 Hypothetical protein MGC34680 Hypothetical protein MGC34680 membrane n/a n/a n/a n/a n/a 1 n/a 1 n/a n/a 1 n/a n/a n/a 1 n/a n/a n/a n/a n/a integral membrane protein n/a found at its expected molecular weight found at molecular weight
IPI00171004 Hypothetical protein MGC34680 Hypothetical protein MGC34680 membrane n/a n/a n/a n/a n/a 1 n/a 1 n/a n/a 1 n/a n/a n/a 1 n/a n/a n/a n/a n/a integral membrane protein n/a found at its expected molecular weight found at molecular weight
UniProt
Q8N370
ID LAT4_HUMAN Reviewed; 569 AA.
AC Q8N370; D3DTH9; Q5CD75; Q6IPM1; Q8NBX1; Q8NC21; Q8WZ00;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2002, sequence version 1.
DT 22-JAN-2014, entry version 86.
DE RecName: Full=Large neutral amino acids transporter small subunit 4;
DE AltName: Full=L-type amino acid transporter 4;
DE AltName: Full=Solute carrier family 43 member 2;
GN Name=SLC43A2; Synonyms=LAT4; ORFNames=PP7664;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION,
RP AND MUTAGENESIS OF SER-297.
RX PubMed=15659399; DOI=10.1074/jbc.M408638200;
RA Bodoy S., Martin L., Zorzano A., Palacin M., Estevez R., Bertran J.;
RT "Identification of LAT4, a novel amino acid transporter with system L
RT activity.";
RL J. Biol. Chem. 280:12002-12011(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RC TISSUE=Frontal cortex;
RA Shadi B., Kanai Y., Iribe Y., Chairoungdua A., Babu E., Jutabha P.,
RA Li Y., Anzai N., Endou H.;
RT "Identification of a human system L2 neutral amino acid transporter in
RT brain.";
RL Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H.,
RA Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y.,
RA Shu H., Chen X., Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S.,
RA Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-569.
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274 AND SER-278, AND
RP MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
CC -!- FUNCTION: Sodium-, chloride-, and pH-independent high affinity
CC transport of large neutral amino acids.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By
CC similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8N370-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N370-2; Sequence=VSP_028665, VSP_028666;
CC Name=3;
CC IsoId=Q8N370-3; Sequence=VSP_028664;
CC -!- TISSUE SPECIFICITY: Detected in several tissues with higher
CC expression in placenta, kidney and peripheral blood leukocytes. In
CC the kidney, is detected in epithelial cells of the distal tubule
CC and collecting duct. In the intestine, is expressed mainly in
CC crypt cells of the intestinal microvilli and epithelial cells in
CC the base of the villus.
CC -!- PTM: Glycosylated (By similarity).
CC -!- SIMILARITY: Belongs to the SLC43A transporter (TC 2.A.1.44)
CC family.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC11383.1; Type=Erroneous initiation;
CC Sequence=BAC11450.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
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CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; BK005642; DAA05676.1; -; mRNA.
DR EMBL; AB120364; BAD91091.1; -; mRNA.
DR EMBL; AF289592; AAL55776.1; -; mRNA.
DR EMBL; CH471108; EAW90602.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90604.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90605.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90607.1; -; Genomic_DNA.
DR EMBL; BC027923; AAH27923.1; -; mRNA.
DR EMBL; BC071859; AAH71859.1; -; mRNA.
DR EMBL; AK075068; BAC11383.1; ALT_INIT; mRNA.
DR EMBL; AK075173; BAC11450.1; ALT_INIT; mRNA.
DR RefSeq; NP_001271427.1; NM_001284498.1.
DR RefSeq; NP_001271428.1; NM_001284499.1.
DR RefSeq; NP_689559.1; NM_152346.2.
DR UniGene; Hs.160550; -.
DR ProteinModelPortal; Q8N370; -.
DR SMR; Q8N370; 157-185.
DR MINT; MINT-4724578; -.
DR STRING; 9606.ENSP00000301335; -.
DR TCDB; 2.A.1.44.2; the major facilitator superfamily (mfs).
DR PhosphoSite; Q8N370; -.
DR DMDM; 74728779; -.
DR PaxDb; Q8N370; -.
DR PRIDE; Q8N370; -.
DR Ensembl; ENST00000301335; ENSP00000301335; ENSG00000167703.
DR Ensembl; ENST00000571650; ENSP00000461382; ENSG00000167703.
DR GeneID; 124935; -.
DR KEGG; hsa:124935; -.
DR UCSC; uc002fsv.3; human.
DR CTD; 124935; -.
DR GeneCards; GC17M001477; -.
DR H-InvDB; HIX0017704; -.
DR H-InvDB; HIX0039573; -.
DR HGNC; HGNC:23087; SLC43A2.
DR HPA; HPA021564; -.
DR MIM; 610791; gene.
DR neXtProt; NX_Q8N370; -.
DR PharmGKB; PA134940765; -.
DR eggNOG; NOG71084; -.
DR HOGENOM; HOG000231937; -.
DR HOVERGEN; HBG072879; -.
DR KO; K08229; -.
DR OMA; FYSYLCK; -.
DR OrthoDB; EOG712TWX; -.
DR PhylomeDB; Q8N370; -.
DR Reactome; REACT_15518; Transmembrane transport of small molecules.
DR Reactome; REACT_19419; Amino acid and oligopeptide SLC transporters.
DR ChiTaRS; SLC43A2; human.
DR GenomeRNAi; 124935; -.
DR NextBio; 81433; -.
DR PRO; PR:Q8N370; -.
DR ArrayExpress; Q8N370; -.
DR Bgee; Q8N370; -.
DR CleanEx; HS_SLC43A2; -.
DR Genevestigator; Q8N370; -.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:0006865; P:amino acid transport; TAS:Reactome.
DR GO; GO:0055085; P:transmembrane transport; TAS:Reactome.
DR InterPro; IPR027201; LAT4.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR016196; MFS_dom_general_subst_transpt.
DR InterPro; IPR027192; SLC43_2/3.
DR PANTHER; PTHR32553; PTHR32553; 1.
DR PANTHER; PTHR32553:SF4; PTHR32553:SF4; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 3.
DR PROSITE; PS50850; MFS; FALSE_NEG.
PE 1: Evidence at protein level;
KW Alternative splicing; Amino-acid transport; Complete proteome;
KW Glycoprotein; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 569 Large neutral amino acids transporter
FT small subunit 4.
FT /FTId=PRO_0000307272.
FT TRANSMEM 20 40 Helical; (Potential).
FT TRANSMEM 90 110 Helical; (Potential).
FT TRANSMEM 120 140 Helical; (Potential).
FT TRANSMEM 143 163 Helical; (Potential).
FT TRANSMEM 177 197 Helical; (Potential).
FT TRANSMEM 203 223 Helical; (Potential).
FT TRANSMEM 323 345 Helical; (Potential).
FT TRANSMEM 365 385 Helical; (Potential).
FT TRANSMEM 435 455 Helical; (Potential).
FT TRANSMEM 462 482 Helical; (Potential).
FT TRANSMEM 490 510 Helical; (Potential).
FT TRANSMEM 516 536 Helical; (Potential).
FT COMPBIAS 409 413 Poly-Lys.
FT MOD_RES 274 274 Phosphoserine.
FT MOD_RES 278 278 Phosphoserine.
FT MOD_RES 297 297 Phosphoserine (By similarity).
FT CARBOHYD 55 55 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 58 58 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 560 560 N-linked (GlcNAc...) (Potential).
FT VAR_SEQ 358 358 K -> KTVMA (in isoform 3).
FT /FTId=VSP_028664.
FT VAR_SEQ 407 489 GEKKKKKRDRQIQKITNAMRAFAFTNLLLVGFGVTCLIPNL
FT PLQILSFILHTIVRGFIHSAVGGLYAAVYPSTQFGSLTGLQ
FT S -> CVGRAGAPAPSPQPLQKDPRAACQAQGGWDRGREQC
FT TPAPPGALRETHSFSSACVSTAPLFMEIVWNAMEMLKFEAR
FT CGDSCL (in isoform 2).
FT /FTId=VSP_028665.
FT VAR_SEQ 490 569 Missing (in isoform 2).
FT /FTId=VSP_028666.
FT MUTAGEN 297 297 S->A: Abolishes sensitivity to N-
FT ethymaleimide.
FT CONFLICT 19 19 A -> P (in Ref. 3; AAL55776).
FT CONFLICT 254 254 D -> N (in Ref. 6; BAC11383).
SQ SEQUENCE 569 AA; 62747 MW; 86C4BB53FF849881 CRC64;
MAPTLATAHR RRWWMACTAV LENLLFSAVL LGWGSLLIML KSEGFYSYLC TEPENVTNGT
VGGTAEPGHE EVSWMNGWLS CQAQDEMLNL AFTVGSFLLS AITLPLGIVM DKYGPRKLRL
LGSACFAVSC LLIAYGASKP NALSVLIFIA LALNGFGGMC MTFTSLTLPN MFGDLRSTFI
ALMIGSYASS AVTFPGIKLI YDAGVSFIVV LVVWAGCSGL VFLNCFFNWP LEPFPGPEDM
DYSVKIKFSW LGFDHKITGK QFYKQVTTVG RRLSVGSSMR SAKEQVALQE GHKLCLSTVD
LEVKCQPDAA VAPSFMHSVF SPILLLSLVT MCVTQLRLIF YMGAMNNILK FLVSGDQKTV
GLYTSIFGVL QLLCLLTAPV IGYIMDWRLK ECEDASEEPE EKDANQGEKK KKKRDRQIQK
ITNAMRAFAF TNLLLVGFGV TCLIPNLPLQ ILSFILHTIV RGFIHSAVGG LYAAVYPSTQ
FGSLTGLQSL ISALFALLQQ PLFLAMMGPL QGDPLWVNVG LLLLSLLGFC LPLYLICYRR
QLERQLQQRQ EDDKLFLKIN GSSNQEAFV
//
ID LAT4_HUMAN Reviewed; 569 AA.
AC Q8N370; D3DTH9; Q5CD75; Q6IPM1; Q8NBX1; Q8NC21; Q8WZ00;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-OCT-2002, sequence version 1.
DT 22-JAN-2014, entry version 86.
DE RecName: Full=Large neutral amino acids transporter small subunit 4;
DE AltName: Full=L-type amino acid transporter 4;
DE AltName: Full=Solute carrier family 43 member 2;
GN Name=SLC43A2; Synonyms=LAT4; ORFNames=PP7664;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION,
RP AND MUTAGENESIS OF SER-297.
RX PubMed=15659399; DOI=10.1074/jbc.M408638200;
RA Bodoy S., Martin L., Zorzano A., Palacin M., Estevez R., Bertran J.;
RT "Identification of LAT4, a novel amino acid transporter with system L
RT activity.";
RL J. Biol. Chem. 280:12002-12011(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RC TISSUE=Frontal cortex;
RA Shadi B., Kanai Y., Iribe Y., Chairoungdua A., Babu E., Jutabha P.,
RA Li Y., Anzai N., Endou H.;
RT "Identification of a human system L2 neutral amino acid transporter in
RT brain.";
RL Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H.,
RA Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y.,
RA Shu H., Chen X., Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S.,
RA Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 90-569.
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274 AND SER-278, AND
RP MASS SPECTROMETRY.
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
CC -!- FUNCTION: Sodium-, chloride-, and pH-independent high affinity
CC transport of large neutral amino acids.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By
CC similarity).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8N370-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N370-2; Sequence=VSP_028665, VSP_028666;
CC Name=3;
CC IsoId=Q8N370-3; Sequence=VSP_028664;
CC -!- TISSUE SPECIFICITY: Detected in several tissues with higher
CC expression in placenta, kidney and peripheral blood leukocytes. In
CC the kidney, is detected in epithelial cells of the distal tubule
CC and collecting duct. In the intestine, is expressed mainly in
CC crypt cells of the intestinal microvilli and epithelial cells in
CC the base of the villus.
CC -!- PTM: Glycosylated (By similarity).
CC -!- SIMILARITY: Belongs to the SLC43A transporter (TC 2.A.1.44)
CC family.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC11383.1; Type=Erroneous initiation;
CC Sequence=BAC11450.1; Type=Erroneous initiation;
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; BK005642; DAA05676.1; -; mRNA.
DR EMBL; AB120364; BAD91091.1; -; mRNA.
DR EMBL; AF289592; AAL55776.1; -; mRNA.
DR EMBL; CH471108; EAW90602.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90604.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90605.1; -; Genomic_DNA.
DR EMBL; CH471108; EAW90607.1; -; Genomic_DNA.
DR EMBL; BC027923; AAH27923.1; -; mRNA.
DR EMBL; BC071859; AAH71859.1; -; mRNA.
DR EMBL; AK075068; BAC11383.1; ALT_INIT; mRNA.
DR EMBL; AK075173; BAC11450.1; ALT_INIT; mRNA.
DR RefSeq; NP_001271427.1; NM_001284498.1.
DR RefSeq; NP_001271428.1; NM_001284499.1.
DR RefSeq; NP_689559.1; NM_152346.2.
DR UniGene; Hs.160550; -.
DR ProteinModelPortal; Q8N370; -.
DR SMR; Q8N370; 157-185.
DR MINT; MINT-4724578; -.
DR STRING; 9606.ENSP00000301335; -.
DR TCDB; 2.A.1.44.2; the major facilitator superfamily (mfs).
DR PhosphoSite; Q8N370; -.
DR DMDM; 74728779; -.
DR PaxDb; Q8N370; -.
DR PRIDE; Q8N370; -.
DR Ensembl; ENST00000301335; ENSP00000301335; ENSG00000167703.
DR Ensembl; ENST00000571650; ENSP00000461382; ENSG00000167703.
DR GeneID; 124935; -.
DR KEGG; hsa:124935; -.
DR UCSC; uc002fsv.3; human.
DR CTD; 124935; -.
DR GeneCards; GC17M001477; -.
DR H-InvDB; HIX0017704; -.
DR H-InvDB; HIX0039573; -.
DR HGNC; HGNC:23087; SLC43A2.
DR HPA; HPA021564; -.
DR MIM; 610791; gene.
DR neXtProt; NX_Q8N370; -.
DR PharmGKB; PA134940765; -.
DR eggNOG; NOG71084; -.
DR HOGENOM; HOG000231937; -.
DR HOVERGEN; HBG072879; -.
DR KO; K08229; -.
DR OMA; FYSYLCK; -.
DR OrthoDB; EOG712TWX; -.
DR PhylomeDB; Q8N370; -.
DR Reactome; REACT_15518; Transmembrane transport of small molecules.
DR Reactome; REACT_19419; Amino acid and oligopeptide SLC transporters.
DR ChiTaRS; SLC43A2; human.
DR GenomeRNAi; 124935; -.
DR NextBio; 81433; -.
DR PRO; PR:Q8N370; -.
DR ArrayExpress; Q8N370; -.
DR Bgee; Q8N370; -.
DR CleanEx; HS_SLC43A2; -.
DR Genevestigator; Q8N370; -.
DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:0006865; P:amino acid transport; TAS:Reactome.
DR GO; GO:0055085; P:transmembrane transport; TAS:Reactome.
DR InterPro; IPR027201; LAT4.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR016196; MFS_dom_general_subst_transpt.
DR InterPro; IPR027192; SLC43_2/3.
DR PANTHER; PTHR32553; PTHR32553; 1.
DR PANTHER; PTHR32553:SF4; PTHR32553:SF4; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 3.
DR PROSITE; PS50850; MFS; FALSE_NEG.
PE 1: Evidence at protein level;
KW Alternative splicing; Amino-acid transport; Complete proteome;
KW Glycoprotein; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1 569 Large neutral amino acids transporter
FT small subunit 4.
FT /FTId=PRO_0000307272.
FT TRANSMEM 20 40 Helical; (Potential).
FT TRANSMEM 90 110 Helical; (Potential).
FT TRANSMEM 120 140 Helical; (Potential).
FT TRANSMEM 143 163 Helical; (Potential).
FT TRANSMEM 177 197 Helical; (Potential).
FT TRANSMEM 203 223 Helical; (Potential).
FT TRANSMEM 323 345 Helical; (Potential).
FT TRANSMEM 365 385 Helical; (Potential).
FT TRANSMEM 435 455 Helical; (Potential).
FT TRANSMEM 462 482 Helical; (Potential).
FT TRANSMEM 490 510 Helical; (Potential).
FT TRANSMEM 516 536 Helical; (Potential).
FT COMPBIAS 409 413 Poly-Lys.
FT MOD_RES 274 274 Phosphoserine.
FT MOD_RES 278 278 Phosphoserine.
FT MOD_RES 297 297 Phosphoserine (By similarity).
FT CARBOHYD 55 55 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 58 58 N-linked (GlcNAc...) (Potential).
FT CARBOHYD 560 560 N-linked (GlcNAc...) (Potential).
FT VAR_SEQ 358 358 K -> KTVMA (in isoform 3).
FT /FTId=VSP_028664.
FT VAR_SEQ 407 489 GEKKKKKRDRQIQKITNAMRAFAFTNLLLVGFGVTCLIPNL
FT PLQILSFILHTIVRGFIHSAVGGLYAAVYPSTQFGSLTGLQ
FT S -> CVGRAGAPAPSPQPLQKDPRAACQAQGGWDRGREQC
FT TPAPPGALRETHSFSSACVSTAPLFMEIVWNAMEMLKFEAR
FT CGDSCL (in isoform 2).
FT /FTId=VSP_028665.
FT VAR_SEQ 490 569 Missing (in isoform 2).
FT /FTId=VSP_028666.
FT MUTAGEN 297 297 S->A: Abolishes sensitivity to N-
FT ethymaleimide.
FT CONFLICT 19 19 A -> P (in Ref. 3; AAL55776).
FT CONFLICT 254 254 D -> N (in Ref. 6; BAC11383).
SQ SEQUENCE 569 AA; 62747 MW; 86C4BB53FF849881 CRC64;
MAPTLATAHR RRWWMACTAV LENLLFSAVL LGWGSLLIML KSEGFYSYLC TEPENVTNGT
VGGTAEPGHE EVSWMNGWLS CQAQDEMLNL AFTVGSFLLS AITLPLGIVM DKYGPRKLRL
LGSACFAVSC LLIAYGASKP NALSVLIFIA LALNGFGGMC MTFTSLTLPN MFGDLRSTFI
ALMIGSYASS AVTFPGIKLI YDAGVSFIVV LVVWAGCSGL VFLNCFFNWP LEPFPGPEDM
DYSVKIKFSW LGFDHKITGK QFYKQVTTVG RRLSVGSSMR SAKEQVALQE GHKLCLSTVD
LEVKCQPDAA VAPSFMHSVF SPILLLSLVT MCVTQLRLIF YMGAMNNILK FLVSGDQKTV
GLYTSIFGVL QLLCLLTAPV IGYIMDWRLK ECEDASEEPE EKDANQGEKK KKKRDRQIQK
ITNAMRAFAF TNLLLVGFGV TCLIPNLPLQ ILSFILHTIV RGFIHSAVGG LYAAVYPSTQ
FGSLTGLQSL ISALFALLQQ PLFLAMMGPL QGDPLWVNVG LLLLSLLGFC LPLYLICYRR
QLERQLQQRQ EDDKLFLKIN GSSNQEAFV
//
MIM
610791
*RECORD*
*FIELD* NO
610791
*FIELD* TI
*610791 SOLUTE CARRIER FAMILY 43 (L-TYPE AMINO ACID TRANSPORTER), MEMBER 2;
SLC43A2
read more;;L-TYPE AMINO ACID TRANSPORTER 4; LAT4
*FIELD* TX
DESCRIPTION
System L amino acid transporters, such as SLC43A2, mediate
sodium-independent transport of bulky neutral amino acids across cell
membranes (Bodoy et al., 2005).
CLONING
By searching a database for sequences similar to LAT3 (SLC43A1; 603733),
Bodoy et al. (2005) identified SLC43A2, which they called LAT4. The
deduced 569-amino acid protein has a calculated molecular mass of 62.7
kD. LAT4 has 12 transmembrane domains with intracellular N and C
termini, and a putative N-glycosylation site is located in the
extracellular loop between the first 2 transmembrane domains. It shares
57% amino acid identity with LAT3 and 91% identity with mouse Lat4.
Northern blot analysis of human tissues detected transcripts of 3.1 and
8 to 9 kb, with highest expression in placenta, followed by kidney and
peripheral blood leukocytes. In mouse tissues, expression was highest in
small intestine. Immunofluorescence analysis detected LAT4 on the plasma
membrane of transfected HeLa cells. In situ hybridization of human
kidney showed LAT4 mRNA restricted to epithelial cells of the distal
tubule and collecting duct. In mouse intestine, Lat4 was mainly
expressed in crypt cells of the intestinal microvilli and in epithelial
cells at the base of the villus. Western blot analysis of transfected
HeLa cells showed that endoglycosidase treatment reduced the molecular
mass of LAT4, confirming that LAT4 is glycosylated.
GENE FUNCTION
Bodoy et al. (2005) found that injection of human LAT4 cRNA into Xenopus
oocytes resulted in increased uptake of L-phenylalanine, L-leucine,
L-isoleucine, and L-methionine. L-phenylalanine transport was
independent of chloride, sodium, and pH. Kinetic analysis of
L-phenylalanine and L-leucine transport activity revealed a low-affinity
component that was sensitive to the sulfhydryl-specific reagent
N-ethylmaleimide and a high-affinity component that was not.
Substitution of the conserved ser297 in the large intracellular loop of
LAT4 with ala partially inhibited transport activity and abolished the
sensitivity to N-ethylmaleimide. Lat4 activity localized to basolateral
membranes in cultured mouse proximal collecting tubule kidney cells.
MAPPING
Hartz (2007) mapped the SLC43A2 gene to chromosome 17p13.3 based on an
alignment of the SLC43A2 sequence (GenBank GENBANK AB120364) with the
genomic sequence (build 36.1).
*FIELD* RF
1. Bodoy, S.; Martin, L.; Zorzano, A.; Palacin, M.; Estevez, R.; Bertran,
J.: Identification of LAT4, a novel amino acid transporter with system
L activity. J. Biol. Chem. 280: 12002-12011, 2005.
2. Hartz, P. A.: Personal Communication. Baltimore, Md. 2/22/2007.
*FIELD* CD
Patricia A. Hartz: 2/22/2007
*FIELD* ED
mgross: 02/22/2007
*RECORD*
*FIELD* NO
610791
*FIELD* TI
*610791 SOLUTE CARRIER FAMILY 43 (L-TYPE AMINO ACID TRANSPORTER), MEMBER 2;
SLC43A2
read more;;L-TYPE AMINO ACID TRANSPORTER 4; LAT4
*FIELD* TX
DESCRIPTION
System L amino acid transporters, such as SLC43A2, mediate
sodium-independent transport of bulky neutral amino acids across cell
membranes (Bodoy et al., 2005).
CLONING
By searching a database for sequences similar to LAT3 (SLC43A1; 603733),
Bodoy et al. (2005) identified SLC43A2, which they called LAT4. The
deduced 569-amino acid protein has a calculated molecular mass of 62.7
kD. LAT4 has 12 transmembrane domains with intracellular N and C
termini, and a putative N-glycosylation site is located in the
extracellular loop between the first 2 transmembrane domains. It shares
57% amino acid identity with LAT3 and 91% identity with mouse Lat4.
Northern blot analysis of human tissues detected transcripts of 3.1 and
8 to 9 kb, with highest expression in placenta, followed by kidney and
peripheral blood leukocytes. In mouse tissues, expression was highest in
small intestine. Immunofluorescence analysis detected LAT4 on the plasma
membrane of transfected HeLa cells. In situ hybridization of human
kidney showed LAT4 mRNA restricted to epithelial cells of the distal
tubule and collecting duct. In mouse intestine, Lat4 was mainly
expressed in crypt cells of the intestinal microvilli and in epithelial
cells at the base of the villus. Western blot analysis of transfected
HeLa cells showed that endoglycosidase treatment reduced the molecular
mass of LAT4, confirming that LAT4 is glycosylated.
GENE FUNCTION
Bodoy et al. (2005) found that injection of human LAT4 cRNA into Xenopus
oocytes resulted in increased uptake of L-phenylalanine, L-leucine,
L-isoleucine, and L-methionine. L-phenylalanine transport was
independent of chloride, sodium, and pH. Kinetic analysis of
L-phenylalanine and L-leucine transport activity revealed a low-affinity
component that was sensitive to the sulfhydryl-specific reagent
N-ethylmaleimide and a high-affinity component that was not.
Substitution of the conserved ser297 in the large intracellular loop of
LAT4 with ala partially inhibited transport activity and abolished the
sensitivity to N-ethylmaleimide. Lat4 activity localized to basolateral
membranes in cultured mouse proximal collecting tubule kidney cells.
MAPPING
Hartz (2007) mapped the SLC43A2 gene to chromosome 17p13.3 based on an
alignment of the SLC43A2 sequence (GenBank GENBANK AB120364) with the
genomic sequence (build 36.1).
*FIELD* RF
1. Bodoy, S.; Martin, L.; Zorzano, A.; Palacin, M.; Estevez, R.; Bertran,
J.: Identification of LAT4, a novel amino acid transporter with system
L activity. J. Biol. Chem. 280: 12002-12011, 2005.
2. Hartz, P. A.: Personal Communication. Baltimore, Md. 2/22/2007.
*FIELD* CD
Patricia A. Hartz: 2/22/2007
*FIELD* ED
mgross: 02/22/2007