Full text data of NDUFB2
NDUFB2
[Confidence: low (only semi-automatic identification from reviews)]
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (Complex I-AGGG; CI-AGGG; NADH-ubiquinone oxidoreductase AGGG subunit; Flags: Precursor)
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (Complex I-AGGG; CI-AGGG; NADH-ubiquinone oxidoreductase AGGG subunit; Flags: Precursor)
UniProt
O95178
ID NDUB2_HUMAN Reviewed; 105 AA.
AC O95178; Q6FGI6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-MAY-1999, sequence version 1.
DT 22-JAN-2014, entry version 110.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial;
DE AltName: Full=Complex I-AGGG;
DE Short=CI-AGGG;
DE AltName: Full=NADH-ubiquinone oxidoreductase AGGG subunit;
DE Flags: Precursor;
GN Name=NDUFB2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9878551; DOI=10.1006/bbrc.1998.9786;
RA Loeffen J.L.C.M., Triepels R.H., van den Heuvel L.P., Schuelke M.,
RA Buskens C.A.F., Smeets R.J.P., Trijbels J.M.F., Smeitink J.A.M.;
RT "cDNA of eight nuclear encoded subunits of NADH:ubiquinone
RT oxidoreductase: human complex I cDNA characterization completed.";
RL Biochem. Biophys. Res. Commun. 253:415-422(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Umbilical cord blood;
RX PubMed=11042152; DOI=10.1101/gr.140200;
RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G.,
RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W.,
RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
RT "Cloning and functional analysis of cDNAs with open reading frames for
RT 300 previously undefined genes expressed in CD34+ hematopoietic
RT stem/progenitor cells.";
RL Genome Res. 10:1546-1560(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
RA Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
RA Korn B., Zuo D., Hu Y., LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 34-44.
RC TISSUE=Leukemic T-cell;
RX PubMed=19892738; DOI=10.1073/pnas.0908958106;
RA Xu G., Shin S.B., Jaffrey S.R.;
RT "Global profiling of protease cleavage sites by chemoselective
RT labeling of protein N-termini.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009).
RN [6]
RP MASS SPECTROMETRY, AND IDENTIFICATION IN THE NADH-UBIQUINONE
RP OXIDOREDUCTASE COMPLEX.
RX PubMed=12611891; DOI=10.1074/jbc.C300064200;
RA Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F.,
RA Ghosh S.S., Capaldi R.A.;
RT "The subunit composition of the human NADH dehydrogenase obtained by
RT rapid one-step immunopurification.";
RL J. Biol. Chem. 278:13619-13622(2003).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane
CC respiratory chain NADH dehydrogenase (Complex I), that is believed
CC not to be involved in catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The
CC immediate electron acceptor for the enzyme is believed to be
CC ubiquinone.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral
CC membrane protein; Matrix side.
CC -!- SIMILARITY: Belongs to the complex I NDUFB2 subunit family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF050639; AAD05428.1; -; mRNA.
DR EMBL; AF067166; AAD32450.1; -; mRNA.
DR EMBL; CR542121; CAG46918.1; -; mRNA.
DR EMBL; BC001168; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; JE0384; JE0384.
DR RefSeq; NP_004537.1; NM_004546.2.
DR UniGene; Hs.655788; -.
DR ProteinModelPortal; O95178; -.
DR IntAct; O95178; 1.
DR STRING; 9606.ENSP00000247866; -.
DR ChEMBL; CHEMBL2363065; -.
DR DrugBank; DB00157; NADH.
DR PhosphoSite; O95178; -.
DR PaxDb; O95178; -.
DR PRIDE; O95178; -.
DR Ensembl; ENST00000247866; ENSP00000247866; ENSG00000090266.
DR Ensembl; ENST00000465506; ENSP00000419357; ENSG00000090266.
DR Ensembl; ENST00000476279; ENSP00000419087; ENSG00000090266.
DR GeneID; 4708; -.
DR KEGG; hsa:4708; -.
DR UCSC; uc003vwa.3; human.
DR CTD; 4708; -.
DR GeneCards; GC07P140390; -.
DR HGNC; HGNC:7697; NDUFB2.
DR MIM; 603838; gene.
DR neXtProt; NX_O95178; -.
DR PharmGKB; PA31503; -.
DR eggNOG; NOG82033; -.
DR HOGENOM; HOG000252974; -.
DR HOVERGEN; HBG006499; -.
DR InParanoid; O95178; -.
DR KO; K03958; -.
DR OMA; FSATMWF; -.
DR OrthoDB; EOG7H1JNP; -.
DR PhylomeDB; O95178; -.
DR Reactome; REACT_111217; Metabolism.
DR ChiTaRS; NDUFB2; human.
DR GeneWiki; NDUFB2; -.
DR GenomeRNAi; 4708; -.
DR NextBio; 18156; -.
DR PRO; PR:O95178; -.
DR ArrayExpress; O95178; -.
DR Bgee; O95178; -.
DR CleanEx; HS_NDUFB2; -.
DR Genevestigator; O95178; -.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; NAS:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; NAS:UniProtKB.
DR GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR InterPro; IPR026627; NDUFB2.
DR PANTHER; PTHR15223; PTHR15223; 1.
PE 1: Evidence at protein level;
KW Complete proteome; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Respiratory chain; Transit peptide; Transport.
FT TRANSIT 1 33 Mitochondrion.
FT CHAIN 34 105 NADH dehydrogenase [ubiquinone] 1 beta
FT subcomplex subunit 2, mitochondrial.
FT /FTId=PRO_0000020042.
SQ SEQUENCE 105 AA; 12058 MW; 5698368608CDC16F CRC64;
MSALTRLASF ARVGGRLFRS GCARTAGDGG VRHAGGGVHI EPRYRQFPQL TRSQVFQSEF
FSGLMWFWIL WRFWHDSEEV LGHFPYPDPS QWTDEELGIP PDDED
//
ID NDUB2_HUMAN Reviewed; 105 AA.
AC O95178; Q6FGI6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-MAY-1999, sequence version 1.
DT 22-JAN-2014, entry version 110.
DE RecName: Full=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial;
DE AltName: Full=Complex I-AGGG;
DE Short=CI-AGGG;
DE AltName: Full=NADH-ubiquinone oxidoreductase AGGG subunit;
DE Flags: Precursor;
GN Name=NDUFB2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9878551; DOI=10.1006/bbrc.1998.9786;
RA Loeffen J.L.C.M., Triepels R.H., van den Heuvel L.P., Schuelke M.,
RA Buskens C.A.F., Smeets R.J.P., Trijbels J.M.F., Smeitink J.A.M.;
RT "cDNA of eight nuclear encoded subunits of NADH:ubiquinone
RT oxidoreductase: human complex I cDNA characterization completed.";
RL Biochem. Biophys. Res. Commun. 253:415-422(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Umbilical cord blood;
RX PubMed=11042152; DOI=10.1101/gr.140200;
RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G.,
RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W.,
RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
RT "Cloning and functional analysis of cDNAs with open reading frames for
RT 300 previously undefined genes expressed in CD34+ hematopoietic
RT stem/progenitor cells.";
RL Genome Res. 10:1546-1560(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
RA Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
RA Korn B., Zuo D., Hu Y., LaBaer J.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 34-44.
RC TISSUE=Leukemic T-cell;
RX PubMed=19892738; DOI=10.1073/pnas.0908958106;
RA Xu G., Shin S.B., Jaffrey S.R.;
RT "Global profiling of protease cleavage sites by chemoselective
RT labeling of protein N-termini.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:19310-19315(2009).
RN [6]
RP MASS SPECTROMETRY, AND IDENTIFICATION IN THE NADH-UBIQUINONE
RP OXIDOREDUCTASE COMPLEX.
RX PubMed=12611891; DOI=10.1074/jbc.C300064200;
RA Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F.,
RA Ghosh S.S., Capaldi R.A.;
RT "The subunit composition of the human NADH dehydrogenase obtained by
RT rapid one-step immunopurification.";
RL J. Biol. Chem. 278:13619-13622(2003).
CC -!- FUNCTION: Accessory subunit of the mitochondrial membrane
CC respiratory chain NADH dehydrogenase (Complex I), that is believed
CC not to be involved in catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The
CC immediate electron acceptor for the enzyme is believed to be
CC ubiquinone.
CC -!- SUBUNIT: Complex I is composed of 45 different subunits.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral
CC membrane protein; Matrix side.
CC -!- SIMILARITY: Belongs to the complex I NDUFB2 subunit family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF050639; AAD05428.1; -; mRNA.
DR EMBL; AF067166; AAD32450.1; -; mRNA.
DR EMBL; CR542121; CAG46918.1; -; mRNA.
DR EMBL; BC001168; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; JE0384; JE0384.
DR RefSeq; NP_004537.1; NM_004546.2.
DR UniGene; Hs.655788; -.
DR ProteinModelPortal; O95178; -.
DR IntAct; O95178; 1.
DR STRING; 9606.ENSP00000247866; -.
DR ChEMBL; CHEMBL2363065; -.
DR DrugBank; DB00157; NADH.
DR PhosphoSite; O95178; -.
DR PaxDb; O95178; -.
DR PRIDE; O95178; -.
DR Ensembl; ENST00000247866; ENSP00000247866; ENSG00000090266.
DR Ensembl; ENST00000465506; ENSP00000419357; ENSG00000090266.
DR Ensembl; ENST00000476279; ENSP00000419087; ENSG00000090266.
DR GeneID; 4708; -.
DR KEGG; hsa:4708; -.
DR UCSC; uc003vwa.3; human.
DR CTD; 4708; -.
DR GeneCards; GC07P140390; -.
DR HGNC; HGNC:7697; NDUFB2.
DR MIM; 603838; gene.
DR neXtProt; NX_O95178; -.
DR PharmGKB; PA31503; -.
DR eggNOG; NOG82033; -.
DR HOGENOM; HOG000252974; -.
DR HOVERGEN; HBG006499; -.
DR InParanoid; O95178; -.
DR KO; K03958; -.
DR OMA; FSATMWF; -.
DR OrthoDB; EOG7H1JNP; -.
DR PhylomeDB; O95178; -.
DR Reactome; REACT_111217; Metabolism.
DR ChiTaRS; NDUFB2; human.
DR GeneWiki; NDUFB2; -.
DR GenomeRNAi; 4708; -.
DR NextBio; 18156; -.
DR PRO; PR:O95178; -.
DR ArrayExpress; O95178; -.
DR Bgee; O95178; -.
DR CleanEx; HS_NDUFB2; -.
DR Genevestigator; O95178; -.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; NAS:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; NAS:UniProtKB.
DR GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR InterPro; IPR026627; NDUFB2.
DR PANTHER; PTHR15223; PTHR15223; 1.
PE 1: Evidence at protein level;
KW Complete proteome; Direct protein sequencing; Electron transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Respiratory chain; Transit peptide; Transport.
FT TRANSIT 1 33 Mitochondrion.
FT CHAIN 34 105 NADH dehydrogenase [ubiquinone] 1 beta
FT subcomplex subunit 2, mitochondrial.
FT /FTId=PRO_0000020042.
SQ SEQUENCE 105 AA; 12058 MW; 5698368608CDC16F CRC64;
MSALTRLASF ARVGGRLFRS GCARTAGDGG VRHAGGGVHI EPRYRQFPQL TRSQVFQSEF
FSGLMWFWIL WRFWHDSEEV LGHFPYPDPS QWTDEELGIP PDDED
//
MIM
603838
*RECORD*
*FIELD* NO
603838
*FIELD* TI
*603838 NADH-UBIQUINONE OXIDOREDUCTASE 1 BETA SUBCOMPLEX, 2; NDUFB2
*FIELD* TX
DESCRIPTION
read more
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first
enzyme complex in the electron transport chain of mitochondria. NDUFB2
is a nuclear encoded subunit located in the hydrophobic protein fraction
of complex I (Loeffen et al., 1998).
CLONING
By EST database searching and PCR, Loeffen et al. (1998) isolated human
cDNAs encoding NDUFB2 and 7 other complex I subunits. The predicted
105-amino acid human protein is 84% identical to Aggg, its bovine
homolog. Hydropathy analysis revealed that NDUFB2 and 4 other subunits
had an overall hydrophilic pattern, even though they were found within
the hydrophobic protein fraction of complex I.
*FIELD* RF
1. Loeffen, J. L. C. M.; Triepels, R. H.; van den Heuvel, L. P.; Schuelke,
M.; Buskens, C. A. F.; Smeets, R. J. P.; Trijbels, J. M. F.; Smeitink,
J. A. M.: cDNA of eight nuclear encoded subunits of NADH:ubiquinone
oxidoreductase: human complex I cDNA characterization completed. Biochem.
Biophys. Res. Commun. 253: 415-422, 1998.
*FIELD* CD
Rebekah S. Rasooly: 5/25/1999
*FIELD* ED
mgross: 02/08/2008
terry: 2/7/2008
alopez: 5/25/1999
*RECORD*
*FIELD* NO
603838
*FIELD* TI
*603838 NADH-UBIQUINONE OXIDOREDUCTASE 1 BETA SUBCOMPLEX, 2; NDUFB2
*FIELD* TX
DESCRIPTION
read more
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first
enzyme complex in the electron transport chain of mitochondria. NDUFB2
is a nuclear encoded subunit located in the hydrophobic protein fraction
of complex I (Loeffen et al., 1998).
CLONING
By EST database searching and PCR, Loeffen et al. (1998) isolated human
cDNAs encoding NDUFB2 and 7 other complex I subunits. The predicted
105-amino acid human protein is 84% identical to Aggg, its bovine
homolog. Hydropathy analysis revealed that NDUFB2 and 4 other subunits
had an overall hydrophilic pattern, even though they were found within
the hydrophobic protein fraction of complex I.
*FIELD* RF
1. Loeffen, J. L. C. M.; Triepels, R. H.; van den Heuvel, L. P.; Schuelke,
M.; Buskens, C. A. F.; Smeets, R. J. P.; Trijbels, J. M. F.; Smeitink,
J. A. M.: cDNA of eight nuclear encoded subunits of NADH:ubiquinone
oxidoreductase: human complex I cDNA characterization completed. Biochem.
Biophys. Res. Commun. 253: 415-422, 1998.
*FIELD* CD
Rebekah S. Rasooly: 5/25/1999
*FIELD* ED
mgross: 02/08/2008
terry: 2/7/2008
alopez: 5/25/1999