Full text data of LCP1
LCP1
(PLS2)
[Confidence: high (present in two of the MS resources)]
Plastin-2 (L-plastin; LC64P; Lymphocyte cytosolic protein 1; LCP-1)
Plastin-2 (L-plastin; LC64P; Lymphocyte cytosolic protein 1; LCP-1)
hRBCD
IPI00010471
IPI00010471 L-plas Actin-binding protein, lymph soluble n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a cytoplasmic n/a expected molecular weight found in band found in band around 188 kDa and in band around 98 kDa
IPI00010471 L-plas Actin-binding protein, lymph soluble n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a n/a cytoplasmic n/a expected molecular weight found in band found in band around 188 kDa and in band around 98 kDa
UniProt
P13796
ID PLSL_HUMAN Reviewed; 627 AA.
AC P13796; B2R613; Q5TBN4;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
read moreDT 05-OCT-2010, sequence version 6.
DT 22-JAN-2014, entry version 165.
DE RecName: Full=Plastin-2;
DE AltName: Full=L-plastin;
DE AltName: Full=LC64P;
DE AltName: Full=Lymphocyte cytosolic protein 1;
DE Short=LCP-1;
GN Name=LCP1; Synonyms=PLS2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT GLU-533.
RX PubMed=2252891; DOI=10.1021/bi00488a017;
RA Zu Y., Shigesada K., Nishida E., Kubota I., Kohno M., Hanaoka M.,
RA Namba Y.;
RT "65-kilodalton protein phosphorylated by interleukin 2 stimulation
RT bears two putative actin-binding sites and two calcium-binding
RT sites.";
RL Biochemistry 29:8319-8324(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-533.
RC TISSUE=Amygdala;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E.,
RA Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.,
RA Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R.,
RA Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S.,
RA Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M.,
RA Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J.,
RA Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E.,
RA Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L.,
RA Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J.,
RA Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S.,
RA Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J.,
RA Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M.,
RA King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A.,
RA Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S.,
RA Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I.,
RA Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S.,
RA Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A.,
RA Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B.,
RA Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L.,
RA Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M.,
RA Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-533.
RC TISSUE=Lymph, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 58-627, PARTIAL PROTEIN SEQUENCE, AND
RP VARIANT GLU-533.
RX PubMed=3211125;
RA Lin C.-S., Aebersold R.H., Kent S.B., Varma M., Leavitt J.;
RT "Molecular cloning and characterization of plastin, a human leukocyte
RT protein expressed in transformed human fibroblasts.";
RL Mol. Cell. Biol. 8:4659-4668(1988).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-140, AND SEQUENCE REVISION.
RX PubMed=2378651;
RA Lin C.-S., Aebersold R.H., Leavitt J.;
RT "Correction of the N-terminal sequences of the human plastin isoforms
RT by using anchored polymerase chain reaction: identification of a
RT potential calcium-binding domain.";
RL Mol. Cell. Biol. 10:1818-1821(1990).
RN [8]
RP PROTEIN SEQUENCE OF 2-14; 20-31; 184-192 AND 534-541, CLEAVAGE OF
RP INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
RC TISSUE=T-cell;
RA Bienvenut W.V., Kanor S., Tissot J.-D., Quadroni M.;
RL Submitted (MAY-2006) to UniProtKB.
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 585-627.
RX PubMed=8428952;
RA Lin C.-S., Park T., Chen Z.P., Leavitt J.;
RT "Human plastin genes. Comparative gene structure, chromosome location,
RT and differential expression in normal and neoplastic cells.";
RL J. Biol. Chem. 268:2781-2792(1993).
RN [10]
RP PROTEIN SEQUENCE OF 264-283; 517-533 AND 593-609, AND VARIANT GLU-533.
RX PubMed=2111166; DOI=10.1021/bi00456a030;
RA Zu Y., Kohno M., Kubota I., Nishida E., Hanaoka M., Namba Y.;
RT "Characterization of interleukin 2 stimulated 65-kilodalton
RT phosphoprotein in human T cells.";
RL Biochemistry 29:1055-1062(1990).
RN [11]
RP PROTEIN SEQUENCE OF 590-611, AND TISSUE SPECIFICITY.
RX PubMed=3261603; DOI=10.1021/bi00410a037;
RA Matsushima K., Shiroo M., Kung H.F., Copeland T.D.;
RT "Purification and characterization of a cytosolic 65-kilodalton
RT phosphoprotein in human leukocytes whose phosphorylation is augmented
RT by stimulation with interleukin 1.";
RL Biochemistry 27:3765-3770(1988).
RN [12]
RP INVOLVEMENT IN B-CELL NON-HODGKIN LYMPHOMA, AND CHROMOSOMAL
RP TRANSLOCATION WITH BCL6.
RX PubMed=10469447;
RX DOI=10.1002/(SICI)1098-2264(199910)26:2<97::AID-GCC1>3.3.CO;2-0;
RA Galiegue-Zouitina S., Quief S., Hildebrand M.P., Denis C.,
RA Detourmignies L., Lai J.L., Kerckaert J.P.;
RT "Nonrandom fusion of L-plastin(LCP1) and LAZ3(BCL6) genes by
RT t(3;13)(q27;q14) chromosome translocation in two cases of B-cell non-
RT Hodgkin lymphoma.";
RL Genes Chromosomes Cancer 26:97-105(1999).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-124, AND MASS
RP SPECTROMETRY.
RX PubMed=15592455; DOI=10.1038/nbt1046;
RA Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,
RA Zha X.-M., Polakiewicz R.D., Comb M.J.;
RT "Immunoaffinity profiling of tyrosine phosphorylation in cancer
RT cells.";
RL Nat. Biotechnol. 23:94-101(2005).
RN [14]
RP FUNCTION, ACTIN-BINDING, INTERACTION WITH ACTIN FIBERS, SUBCELLULAR
RP LOCATION, MUTAGENESIS OF SER-5, AND PHOSPHORYLATION AT SER-5.
RX PubMed=16636079; DOI=10.1242/jcs.02874;
RA Janji B., Giganti A., De Corte V., Catillon M., Bruyneel E., Lentz D.,
RA Plastino J., Gettemans J., Friederich E.;
RT "Phosphorylation on Ser5 increases the F-actin-binding activity of L-
RT plastin and promotes its targeting to sites of actin assembly in
RT cells.";
RL J. Cell Sci. 119:1947-1960(2006).
RN [15]
RP SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF SER-5, PHOSPHORYLATION
RP AT SER-5, AND MASS SPECTROMETRY.
RX PubMed=17294403; DOI=10.1002/eji.200636320;
RA Wabnitz G.H., Koecher T., Lohneis P., Stober C., Konstandin M.H.,
RA Funk B., Sester U., Wilm M., Klemke M., Samstag Y.;
RT "Costimulation induced phosphorylation of L-plastin facilitates
RT surface transport of the T cell activation molecules CD69 and CD25.";
RL Eur. J. Immunol. 37:649-662(2007).
RN [16]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=T-cell;
RX PubMed=19367720; DOI=10.1021/pr800500r;
RA Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.;
RT "Phosphorylation analysis of primary human T lymphocytes using
RT sequential IMAC and titanium oxide enrichment.";
RL J. Proteome Res. 7:5167-5176(2008).
RN [17]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; TYR-28; SER-30;
RP SER-257 AND SER-323, AND MASS SPECTROMETRY.
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [18]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-76; LYS-88; LYS-294;
RP LYS-297; LYS-361; LYS-472; LYS-542 AND LYS-579, AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [19]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [20]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5, AND MASS
RP SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [21]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [22]
RP STRUCTURE BY NMR OF 513-627.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the fourth CH domain from human L-plastin.";
RL Submitted (JUN-2006) to the PDB data bank.
CC -!- FUNCTION: Actin-binding protein. Plays a role in the activation of
CC T-cells in response to costimulation through TCR/CD3 and CD2 or
CC CD28. Modulates the cell surface expression of IL2RA/CD25 and
CC CD69.
CC -!- SUBUNIT: Monomer. Interacts with AIF1 (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction. Cell
CC projection. Cell projection, ruffle membrane; Peripheral membrane
CC protein; Cytoplasmic side (By similarity). Note=Relocalizes to the
CC immunological synapse between peripheral blood T-lymphocytes and
CC antibody-presenting cells in response to costimulation through
CC TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at
CC membrane ruffles (By similarity). Relocalizes to actin-rich cell
CC projections upon serine phosphorylation.
CC -!- TISSUE SPECIFICITY: Detected in intestinal microvilli, hair cell
CC stereocilia, and fibroblast filopodia, in spleen and other lymph
CC node-containing organs. Expressed in peripheral blood T-
CC lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid
CC cells.
CC -!- PTM: Phosphorylated on a serine residue in response to
CC costimulation through TCR/CD3 and CD2 or CD28. Serine
CC phosphorylation promotes association with the actin cytoskeleton
CC and targeting to peripheral cell projections.
CC -!- DISEASE: Note=Chromosomal aberrations involving LCP1 is a cause of
CC B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation
CC t(3;13)(q27;q14), with BCL6.
CC -!- SIMILARITY: Contains 2 actin-binding domains.
CC -!- SIMILARITY: Contains 4 CH (calponin-homology) domains.
CC -!- SIMILARITY: Contains 2 EF-hand domains.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology
CC and Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/LCP1ID95.html";
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DR EMBL; J02923; AAA63236.1; -; mRNA.
DR EMBL; AK312393; BAG35310.1; -; mRNA.
DR EMBL; AL137141; CAI12168.2; -; Genomic_DNA.
DR EMBL; CH471075; EAX08757.1; -; Genomic_DNA.
DR EMBL; BC007673; AAH07673.1; -; mRNA.
DR EMBL; BC010271; AAH10271.1; -; mRNA.
DR EMBL; M22300; AAB02845.1; -; mRNA.
DR EMBL; AH002870; AAA59529.1; -; Genomic_DNA.
DR EMBL; M34426; AAA36184.1; -; mRNA.
DR PIR; A35836; A35836.
DR RefSeq; NP_002289.2; NM_002298.4.
DR RefSeq; XP_005266431.1; XM_005266374.1.
DR UniGene; Hs.381099; -.
DR UniGene; Hs.658408; -.
DR PDB; 2D85; NMR; -; A=517-627.
DR PDBsum; 2D85; -.
DR ProteinModelPortal; P13796; -.
DR SMR; P13796; 7-77, 118-627.
DR IntAct; P13796; 7.
DR MINT; MINT-3008082; -.
DR STRING; 9606.ENSP00000315757; -.
DR PhosphoSite; P13796; -.
DR DMDM; 308153685; -.
DR PaxDb; P13796; -.
DR PRIDE; P13796; -.
DR DNASU; 3936; -.
DR Ensembl; ENST00000323076; ENSP00000315757; ENSG00000136167.
DR Ensembl; ENST00000398576; ENSP00000381581; ENSG00000136167.
DR GeneID; 3936; -.
DR KEGG; hsa:3936; -.
DR UCSC; uc001vaz.4; human.
DR CTD; 3936; -.
DR GeneCards; GC13M046700; -.
DR H-InvDB; HIX0130592; -.
DR HGNC; HGNC:6528; LCP1.
DR HPA; CAB020673; -.
DR HPA; HPA019493; -.
DR MIM; 153430; gene.
DR neXtProt; NX_P13796; -.
DR PharmGKB; PA30312; -.
DR eggNOG; COG5069; -.
DR HOGENOM; HOG000213447; -.
DR HOVERGEN; HBG003082; -.
DR InParanoid; P13796; -.
DR KO; K17276; -.
DR OMA; HLYADID; -.
DR OrthoDB; EOG7K9K2D; -.
DR PhylomeDB; P13796; -.
DR ChiTaRS; LCP1; human.
DR EvolutionaryTrace; P13796; -.
DR GeneWiki; LCP1; -.
DR GenomeRNAi; 3936; -.
DR NextBio; 15457; -.
DR PRO; PR:P13796; -.
DR ArrayExpress; P13796; -.
DR Bgee; P13796; -.
DR CleanEx; HS_LCP1; -.
DR Genevestigator; P13796; -.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:HPA.
DR GO; GO:0005884; C:actin filament; IEA:Ensembl.
DR GO; GO:0030054; C:cell junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0001891; C:phagocytic cup; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003779; F:actin binding; NAS:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; NAS:UniProtKB.
DR GO; GO:0051017; P:actin filament bundle assembly; IEA:Ensembl.
DR GO; GO:0031100; P:organ regeneration; IEA:Ensembl.
DR GO; GO:0033157; P:regulation of intracellular protein transport; IDA:UniProtKB.
DR GO; GO:0002286; P:T cell activation involved in immune response; IDA:UniProtKB.
DR Gene3D; 1.10.238.10; -; 1.
DR Gene3D; 1.10.418.10; -; 4.
DR InterPro; IPR001589; Actinin_actin-bd_CS.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF00307; CH; 4.
DR Pfam; PF00036; EF-hand_1; 1.
DR SMART; SM00033; CH; 4.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS00019; ACTININ_1; 2.
DR PROSITE; PS00020; ACTININ_2; 2.
DR PROSITE; PS50021; CH; 4.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Actin-binding; Calcium; Cell junction;
KW Cell membrane; Cell projection; Chromosomal rearrangement;
KW Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW Membrane; Metal-binding; Phosphoprotein; Polymorphism;
KW Reference proteome; Repeat.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 627 Plastin-2.
FT /FTId=PRO_0000073743.
FT DOMAIN 9 44 EF-hand 1.
FT DOMAIN 49 84 EF-hand 2.
FT DOMAIN 106 379 Actin-binding 1.
FT DOMAIN 120 236 CH 1.
FT DOMAIN 264 375 CH 2.
FT DOMAIN 380 624 Actin-binding 2.
FT DOMAIN 394 503 CH 3.
FT DOMAIN 515 624 CH 4.
FT CA_BIND 22 33 1 (By similarity).
FT CA_BIND 62 73 2 (By similarity).
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 5 5 Phosphoserine.
FT MOD_RES 7 7 Phosphoserine.
FT MOD_RES 28 28 Phosphotyrosine.
FT MOD_RES 30 30 Phosphoserine.
FT MOD_RES 76 76 N6-acetyllysine.
FT MOD_RES 88 88 N6-acetyllysine.
FT MOD_RES 124 124 Phosphotyrosine.
FT MOD_RES 257 257 Phosphoserine.
FT MOD_RES 294 294 N6-acetyllysine.
FT MOD_RES 297 297 N6-acetyllysine.
FT MOD_RES 323 323 Phosphoserine.
FT MOD_RES 361 361 N6-acetyllysine.
FT MOD_RES 472 472 N6-acetyllysine.
FT MOD_RES 542 542 N6-acetyllysine.
FT MOD_RES 579 579 N6-acetyllysine.
FT VARIANT 24 24 D -> E.
FT /FTId=VAR_001371.
FT VARIANT 533 533 K -> E (in dbSNP:rs4941543).
FT /FTId=VAR_024398.
FT VARIANT 544 544 P -> A (in dbSNP:rs17067725).
FT /FTId=VAR_030826.
FT MUTAGEN 5 5 S->A: Abolishes phosphorylation and
FT reduces the cell surface expression of
FT CD69 and IL2RA. Reduces association with
FT the actin cytoskeleton.
FT MUTAGEN 5 5 S->E: Promotes association with the actin
FT cytoskeleton.
FT CONFLICT 611 611 M -> T (in Ref. 11; AA sequence).
FT HELIX 520 532
FT HELIX 545 548
FT HELIX 550 559
FT TURN 566 568
FT HELIX 576 593
FT HELIX 601 604
FT TURN 605 607
FT HELIX 609 612
FT HELIX 615 620
FT TURN 621 623
SQ SEQUENCE 627 AA; 70288 MW; 668E4AA6A3FC7B58 CRC64;
MARGSVSDEE MMELREAFAK VDTDGNGYIS FNELNDLFKA ACLPLPGYRV REITENLMAT
GDLDQDGRIS FDEFIKIFHG LKSTDVAKTF RKAINKKEGI CAIGGTSEQS SVGTQHSYSE
EEKYAFVNWI NKALENDPDC RHVIPMNPNT NDLFNAVGDG IVLCKMINLS VPDTIDERTI
NKKKLTPFTI QENLNLALNS ASAIGCHVVN IGAEDLKEGK PYLVLGLLWQ VIKIGLFADI
ELSRNEALIA LLREGESLED LMKLSPEELL LRWANYHLEN AGCNKIGNFS TDIKDSKAYY
HLLEQVAPKG DEEGVPAVVI DMSGLREKDD IQRAECMLQQ AERLGCRQFV TATDVVRGNP
KLNLAFIANL FNRYPALHKP ENQDIDWGAL EGETREERTF RNWMNSLGVN PRVNHLYSDL
SDALVIFQLY EKIKVPVDWN RVNKPPYPKL GGNMKKLENC NYAVELGKNQ AKFSLVGIGG
QDLNEGNRTL TLALIWQLMR RYTLNILEEI GGGQKVNDDI IVNWVNETLR EAKKSSSISS
FKDPKISTSL PVLDLIDAIQ PGSINYDLLK TENLNDDEKL NNAKYAISMA RKIGARVYAL
PEDLVEVNPK MVMTVFACLM GKGMKRV
//
ID PLSL_HUMAN Reviewed; 627 AA.
AC P13796; B2R613; Q5TBN4;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
read moreDT 05-OCT-2010, sequence version 6.
DT 22-JAN-2014, entry version 165.
DE RecName: Full=Plastin-2;
DE AltName: Full=L-plastin;
DE AltName: Full=LC64P;
DE AltName: Full=Lymphocyte cytosolic protein 1;
DE Short=LCP-1;
GN Name=LCP1; Synonyms=PLS2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT GLU-533.
RX PubMed=2252891; DOI=10.1021/bi00488a017;
RA Zu Y., Shigesada K., Nishida E., Kubota I., Kohno M., Hanaoka M.,
RA Namba Y.;
RT "65-kilodalton protein phosphorylated by interleukin 2 stimulation
RT bears two putative actin-binding sites and two calcium-binding
RT sites.";
RL Biochemistry 29:8319-8324(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-533.
RC TISSUE=Amygdala;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E.,
RA Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.,
RA Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R.,
RA Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S.,
RA Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M.,
RA Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J.,
RA Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E.,
RA Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L.,
RA Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J.,
RA Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S.,
RA Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J.,
RA Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M.,
RA King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A.,
RA Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S.,
RA Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I.,
RA Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S.,
RA Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A.,
RA Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B.,
RA Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L.,
RA Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M.,
RA Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLU-533.
RC TISSUE=Lymph, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 58-627, PARTIAL PROTEIN SEQUENCE, AND
RP VARIANT GLU-533.
RX PubMed=3211125;
RA Lin C.-S., Aebersold R.H., Kent S.B., Varma M., Leavitt J.;
RT "Molecular cloning and characterization of plastin, a human leukocyte
RT protein expressed in transformed human fibroblasts.";
RL Mol. Cell. Biol. 8:4659-4668(1988).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-140, AND SEQUENCE REVISION.
RX PubMed=2378651;
RA Lin C.-S., Aebersold R.H., Leavitt J.;
RT "Correction of the N-terminal sequences of the human plastin isoforms
RT by using anchored polymerase chain reaction: identification of a
RT potential calcium-binding domain.";
RL Mol. Cell. Biol. 10:1818-1821(1990).
RN [8]
RP PROTEIN SEQUENCE OF 2-14; 20-31; 184-192 AND 534-541, CLEAVAGE OF
RP INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
RC TISSUE=T-cell;
RA Bienvenut W.V., Kanor S., Tissot J.-D., Quadroni M.;
RL Submitted (MAY-2006) to UniProtKB.
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 585-627.
RX PubMed=8428952;
RA Lin C.-S., Park T., Chen Z.P., Leavitt J.;
RT "Human plastin genes. Comparative gene structure, chromosome location,
RT and differential expression in normal and neoplastic cells.";
RL J. Biol. Chem. 268:2781-2792(1993).
RN [10]
RP PROTEIN SEQUENCE OF 264-283; 517-533 AND 593-609, AND VARIANT GLU-533.
RX PubMed=2111166; DOI=10.1021/bi00456a030;
RA Zu Y., Kohno M., Kubota I., Nishida E., Hanaoka M., Namba Y.;
RT "Characterization of interleukin 2 stimulated 65-kilodalton
RT phosphoprotein in human T cells.";
RL Biochemistry 29:1055-1062(1990).
RN [11]
RP PROTEIN SEQUENCE OF 590-611, AND TISSUE SPECIFICITY.
RX PubMed=3261603; DOI=10.1021/bi00410a037;
RA Matsushima K., Shiroo M., Kung H.F., Copeland T.D.;
RT "Purification and characterization of a cytosolic 65-kilodalton
RT phosphoprotein in human leukocytes whose phosphorylation is augmented
RT by stimulation with interleukin 1.";
RL Biochemistry 27:3765-3770(1988).
RN [12]
RP INVOLVEMENT IN B-CELL NON-HODGKIN LYMPHOMA, AND CHROMOSOMAL
RP TRANSLOCATION WITH BCL6.
RX PubMed=10469447;
RX DOI=10.1002/(SICI)1098-2264(199910)26:2<97::AID-GCC1>3.3.CO;2-0;
RA Galiegue-Zouitina S., Quief S., Hildebrand M.P., Denis C.,
RA Detourmignies L., Lai J.L., Kerckaert J.P.;
RT "Nonrandom fusion of L-plastin(LCP1) and LAZ3(BCL6) genes by
RT t(3;13)(q27;q14) chromosome translocation in two cases of B-cell non-
RT Hodgkin lymphoma.";
RL Genes Chromosomes Cancer 26:97-105(1999).
RN [13]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-124, AND MASS
RP SPECTROMETRY.
RX PubMed=15592455; DOI=10.1038/nbt1046;
RA Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,
RA Zha X.-M., Polakiewicz R.D., Comb M.J.;
RT "Immunoaffinity profiling of tyrosine phosphorylation in cancer
RT cells.";
RL Nat. Biotechnol. 23:94-101(2005).
RN [14]
RP FUNCTION, ACTIN-BINDING, INTERACTION WITH ACTIN FIBERS, SUBCELLULAR
RP LOCATION, MUTAGENESIS OF SER-5, AND PHOSPHORYLATION AT SER-5.
RX PubMed=16636079; DOI=10.1242/jcs.02874;
RA Janji B., Giganti A., De Corte V., Catillon M., Bruyneel E., Lentz D.,
RA Plastino J., Gettemans J., Friederich E.;
RT "Phosphorylation on Ser5 increases the F-actin-binding activity of L-
RT plastin and promotes its targeting to sites of actin assembly in
RT cells.";
RL J. Cell Sci. 119:1947-1960(2006).
RN [15]
RP SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF SER-5, PHOSPHORYLATION
RP AT SER-5, AND MASS SPECTROMETRY.
RX PubMed=17294403; DOI=10.1002/eji.200636320;
RA Wabnitz G.H., Koecher T., Lohneis P., Stober C., Konstandin M.H.,
RA Funk B., Sester U., Wilm M., Klemke M., Samstag Y.;
RT "Costimulation induced phosphorylation of L-plastin facilitates
RT surface transport of the T cell activation molecules CD69 and CD25.";
RL Eur. J. Immunol. 37:649-662(2007).
RN [16]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=T-cell;
RX PubMed=19367720; DOI=10.1021/pr800500r;
RA Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.;
RT "Phosphorylation analysis of primary human T lymphocytes using
RT sequential IMAC and titanium oxide enrichment.";
RL J. Proteome Res. 7:5167-5176(2008).
RN [17]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7; TYR-28; SER-30;
RP SER-257 AND SER-323, AND MASS SPECTROMETRY.
RC TISSUE=Leukemic T-cell;
RX PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA Rodionov V., Han D.K.;
RT "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT reveals system-wide modulation of protein-protein interactions.";
RL Sci. Signal. 2:RA46-RA46(2009).
RN [18]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-76; LYS-88; LYS-294;
RP LYS-297; LYS-361; LYS-472; LYS-542 AND LYS-579, AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [19]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [20]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5, AND MASS
RP SPECTROMETRY.
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA Blagoev B.;
RT "System-wide temporal characterization of the proteome and
RT phosphoproteome of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [21]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-
RT terminal acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [22]
RP STRUCTURE BY NMR OF 513-627.
RG RIKEN structural genomics initiative (RSGI);
RT "Solution structure of the fourth CH domain from human L-plastin.";
RL Submitted (JUN-2006) to the PDB data bank.
CC -!- FUNCTION: Actin-binding protein. Plays a role in the activation of
CC T-cells in response to costimulation through TCR/CD3 and CD2 or
CC CD28. Modulates the cell surface expression of IL2RA/CD25 and
CC CD69.
CC -!- SUBUNIT: Monomer. Interacts with AIF1 (By similarity).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction. Cell
CC projection. Cell projection, ruffle membrane; Peripheral membrane
CC protein; Cytoplasmic side (By similarity). Note=Relocalizes to the
CC immunological synapse between peripheral blood T-lymphocytes and
CC antibody-presenting cells in response to costimulation through
CC TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at
CC membrane ruffles (By similarity). Relocalizes to actin-rich cell
CC projections upon serine phosphorylation.
CC -!- TISSUE SPECIFICITY: Detected in intestinal microvilli, hair cell
CC stereocilia, and fibroblast filopodia, in spleen and other lymph
CC node-containing organs. Expressed in peripheral blood T-
CC lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid
CC cells.
CC -!- PTM: Phosphorylated on a serine residue in response to
CC costimulation through TCR/CD3 and CD2 or CD28. Serine
CC phosphorylation promotes association with the actin cytoskeleton
CC and targeting to peripheral cell projections.
CC -!- DISEASE: Note=Chromosomal aberrations involving LCP1 is a cause of
CC B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation
CC t(3;13)(q27;q14), with BCL6.
CC -!- SIMILARITY: Contains 2 actin-binding domains.
CC -!- SIMILARITY: Contains 4 CH (calponin-homology) domains.
CC -!- SIMILARITY: Contains 2 EF-hand domains.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology
CC and Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/LCP1ID95.html";
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DR EMBL; J02923; AAA63236.1; -; mRNA.
DR EMBL; AK312393; BAG35310.1; -; mRNA.
DR EMBL; AL137141; CAI12168.2; -; Genomic_DNA.
DR EMBL; CH471075; EAX08757.1; -; Genomic_DNA.
DR EMBL; BC007673; AAH07673.1; -; mRNA.
DR EMBL; BC010271; AAH10271.1; -; mRNA.
DR EMBL; M22300; AAB02845.1; -; mRNA.
DR EMBL; AH002870; AAA59529.1; -; Genomic_DNA.
DR EMBL; M34426; AAA36184.1; -; mRNA.
DR PIR; A35836; A35836.
DR RefSeq; NP_002289.2; NM_002298.4.
DR RefSeq; XP_005266431.1; XM_005266374.1.
DR UniGene; Hs.381099; -.
DR UniGene; Hs.658408; -.
DR PDB; 2D85; NMR; -; A=517-627.
DR PDBsum; 2D85; -.
DR ProteinModelPortal; P13796; -.
DR SMR; P13796; 7-77, 118-627.
DR IntAct; P13796; 7.
DR MINT; MINT-3008082; -.
DR STRING; 9606.ENSP00000315757; -.
DR PhosphoSite; P13796; -.
DR DMDM; 308153685; -.
DR PaxDb; P13796; -.
DR PRIDE; P13796; -.
DR DNASU; 3936; -.
DR Ensembl; ENST00000323076; ENSP00000315757; ENSG00000136167.
DR Ensembl; ENST00000398576; ENSP00000381581; ENSG00000136167.
DR GeneID; 3936; -.
DR KEGG; hsa:3936; -.
DR UCSC; uc001vaz.4; human.
DR CTD; 3936; -.
DR GeneCards; GC13M046700; -.
DR H-InvDB; HIX0130592; -.
DR HGNC; HGNC:6528; LCP1.
DR HPA; CAB020673; -.
DR HPA; HPA019493; -.
DR MIM; 153430; gene.
DR neXtProt; NX_P13796; -.
DR PharmGKB; PA30312; -.
DR eggNOG; COG5069; -.
DR HOGENOM; HOG000213447; -.
DR HOVERGEN; HBG003082; -.
DR InParanoid; P13796; -.
DR KO; K17276; -.
DR OMA; HLYADID; -.
DR OrthoDB; EOG7K9K2D; -.
DR PhylomeDB; P13796; -.
DR ChiTaRS; LCP1; human.
DR EvolutionaryTrace; P13796; -.
DR GeneWiki; LCP1; -.
DR GenomeRNAi; 3936; -.
DR NextBio; 15457; -.
DR PRO; PR:P13796; -.
DR ArrayExpress; P13796; -.
DR Bgee; P13796; -.
DR CleanEx; HS_LCP1; -.
DR Genevestigator; P13796; -.
DR GO; GO:0015629; C:actin cytoskeleton; IDA:HPA.
DR GO; GO:0005884; C:actin filament; IEA:Ensembl.
DR GO; GO:0030054; C:cell junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0001891; C:phagocytic cup; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003779; F:actin binding; NAS:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; NAS:UniProtKB.
DR GO; GO:0051017; P:actin filament bundle assembly; IEA:Ensembl.
DR GO; GO:0031100; P:organ regeneration; IEA:Ensembl.
DR GO; GO:0033157; P:regulation of intracellular protein transport; IDA:UniProtKB.
DR GO; GO:0002286; P:T cell activation involved in immune response; IDA:UniProtKB.
DR Gene3D; 1.10.238.10; -; 1.
DR Gene3D; 1.10.418.10; -; 4.
DR InterPro; IPR001589; Actinin_actin-bd_CS.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF00307; CH; 4.
DR Pfam; PF00036; EF-hand_1; 1.
DR SMART; SM00033; CH; 4.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS00019; ACTININ_1; 2.
DR PROSITE; PS00020; ACTININ_2; 2.
DR PROSITE; PS50021; CH; 4.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Actin-binding; Calcium; Cell junction;
KW Cell membrane; Cell projection; Chromosomal rearrangement;
KW Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing;
KW Membrane; Metal-binding; Phosphoprotein; Polymorphism;
KW Reference proteome; Repeat.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 627 Plastin-2.
FT /FTId=PRO_0000073743.
FT DOMAIN 9 44 EF-hand 1.
FT DOMAIN 49 84 EF-hand 2.
FT DOMAIN 106 379 Actin-binding 1.
FT DOMAIN 120 236 CH 1.
FT DOMAIN 264 375 CH 2.
FT DOMAIN 380 624 Actin-binding 2.
FT DOMAIN 394 503 CH 3.
FT DOMAIN 515 624 CH 4.
FT CA_BIND 22 33 1 (By similarity).
FT CA_BIND 62 73 2 (By similarity).
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 5 5 Phosphoserine.
FT MOD_RES 7 7 Phosphoserine.
FT MOD_RES 28 28 Phosphotyrosine.
FT MOD_RES 30 30 Phosphoserine.
FT MOD_RES 76 76 N6-acetyllysine.
FT MOD_RES 88 88 N6-acetyllysine.
FT MOD_RES 124 124 Phosphotyrosine.
FT MOD_RES 257 257 Phosphoserine.
FT MOD_RES 294 294 N6-acetyllysine.
FT MOD_RES 297 297 N6-acetyllysine.
FT MOD_RES 323 323 Phosphoserine.
FT MOD_RES 361 361 N6-acetyllysine.
FT MOD_RES 472 472 N6-acetyllysine.
FT MOD_RES 542 542 N6-acetyllysine.
FT MOD_RES 579 579 N6-acetyllysine.
FT VARIANT 24 24 D -> E.
FT /FTId=VAR_001371.
FT VARIANT 533 533 K -> E (in dbSNP:rs4941543).
FT /FTId=VAR_024398.
FT VARIANT 544 544 P -> A (in dbSNP:rs17067725).
FT /FTId=VAR_030826.
FT MUTAGEN 5 5 S->A: Abolishes phosphorylation and
FT reduces the cell surface expression of
FT CD69 and IL2RA. Reduces association with
FT the actin cytoskeleton.
FT MUTAGEN 5 5 S->E: Promotes association with the actin
FT cytoskeleton.
FT CONFLICT 611 611 M -> T (in Ref. 11; AA sequence).
FT HELIX 520 532
FT HELIX 545 548
FT HELIX 550 559
FT TURN 566 568
FT HELIX 576 593
FT HELIX 601 604
FT TURN 605 607
FT HELIX 609 612
FT HELIX 615 620
FT TURN 621 623
SQ SEQUENCE 627 AA; 70288 MW; 668E4AA6A3FC7B58 CRC64;
MARGSVSDEE MMELREAFAK VDTDGNGYIS FNELNDLFKA ACLPLPGYRV REITENLMAT
GDLDQDGRIS FDEFIKIFHG LKSTDVAKTF RKAINKKEGI CAIGGTSEQS SVGTQHSYSE
EEKYAFVNWI NKALENDPDC RHVIPMNPNT NDLFNAVGDG IVLCKMINLS VPDTIDERTI
NKKKLTPFTI QENLNLALNS ASAIGCHVVN IGAEDLKEGK PYLVLGLLWQ VIKIGLFADI
ELSRNEALIA LLREGESLED LMKLSPEELL LRWANYHLEN AGCNKIGNFS TDIKDSKAYY
HLLEQVAPKG DEEGVPAVVI DMSGLREKDD IQRAECMLQQ AERLGCRQFV TATDVVRGNP
KLNLAFIANL FNRYPALHKP ENQDIDWGAL EGETREERTF RNWMNSLGVN PRVNHLYSDL
SDALVIFQLY EKIKVPVDWN RVNKPPYPKL GGNMKKLENC NYAVELGKNQ AKFSLVGIGG
QDLNEGNRTL TLALIWQLMR RYTLNILEEI GGGQKVNDDI IVNWVNETLR EAKKSSSISS
FKDPKISTSL PVLDLIDAIQ PGSINYDLLK TENLNDDEKL NNAKYAISMA RKIGARVYAL
PEDLVEVNPK MVMTVFACLM GKGMKRV
//
MIM
153430
*RECORD*
*FIELD* NO
153430
*FIELD* TI
*153430 LYMPHOCYTE CYTOSOLIC PROTEIN 1; LCP1
;;LYMPHOCYTE CYTOSOL POLYPEPTIDE, 64-KD; LC64P; CP64;;
read moreL-PLASTIN; LPL;;
PLASTIN 2; PLS2
*FIELD* TX
CLONING
Lin et al. (1988) isolated partial cDNAs encoding L-plastin and
T-plastin (PLS3; 300131) from a transformed human fibroblast cDNA
library. Northern blot analysis revealed that L-plastin is expressed as
a 3.7-kb mRNA in leukocytes, transformed fibroblasts, and a diverse
array of human tumor cell lines.
Zu et al. (1990) reported that L-plastin is identical to p65, an
interleukin-2 (IL2; 147680)-stimulated phosphoprotein found in human T
cells. Zu et al. (1990) isolated p65 cDNAs from a human T-lymphocyte
cDNA library. The predicted 627-amino acid p65 protein contains 2
EF-hand calcium-binding domains, a calmodulin-binding site, and 2 tandem
repeats of an actin-binding domain.
GENE STRUCTURE
Lin et al. (1993) reported that both the L-plastin and T-plastin genes
contain 16 exons and span approximately 90 kb. Lin et al. (1997) found
that the human and murine L-plastin promoters are highly homologous and
function equally well in either human or murine leukocytes.
MAPPING
Kondo et al. (1985) assigned the LCP1 gene to 13q14.1-q14.2 by the
deletion/dosage method. They studied a patient with trisomy 13 who had
1.5 times the normal amount of protein, a patient with retinoblastoma
and deletion of 13q12.3-q21.2 who had half the normal amount of protein,
and a patient with retinoblastoma and deletion of 13q14.1-q31.2 who had
lost the father's allele and had half the normal amount of protein
(Kondo et al., 1985). Close linkage to ESD (133280) was indicated by a
maximum lod score of 4.221 at zero recombination. As part of an
undertaking to map by genetic linkage human lymphocyte proteins that are
genetically polymorphic in isoelectric point, Goldman et al. (1991)
mapped the LCP1 gene to a site near the ESD locus on chromosome 13 by
studies in 9 families from the CEPH collection. The proteins in the
immortalized lymphocyte cell lines were analyzed by 2-dimensional
electrophoresis. Murayama et al. (1993) demonstrated that L-plastin and
LCP1 are identical and mapped the gene to 13q14.3.
CYTOGENETICS
The LAZ3 gene (BCL6; 109565) on 3q27 is nonrandomly disrupted in B-cell
non-Hodgkin lymphoma by chromosomal translocations. Galiegue-Zouitina et
al. (1999) identified the L-plastin gene as a novel LAZ3 partner in
chimeric transcripts resulting from a t(3;13)(q27;q14) translocation in
2 cases of B-cell lymphoma. As a consequence of the translocation, the
5-prime regulatory region of each gene was exchanged, creating both
LCP1-LAZ3 and reciprocal LAZ3-LCP1 fusion transcripts in one case, and
only an LCP1-LAZ3 fusion transcript in the other.
MOLECULAR GENETICS
Hamaguchi et al. (1982) described a genetic polymorphism of a major
human LCP of molecular weight 64,000, detected in PHA-stimulated
peripheral blood lymphocytes by high-resolution 2-dimensional
electrophoresis (O'Farrell, 1975; Klose, 1975). Three different
phenotypes determined by 2 common alleles at a single locus were found.
In Japanese, the frequency of the 2 alleles was 0.936 and 0.064,
respectively. The polypeptide was not detected in HeLa cells,
fibroblasts, red cells, serum, or cerebrum. Traces were found in liver,
kidney and skeletal muscle. (Hamaguchi et al. (1982) demonstrated
polymorphism in 3 others of about 100 polypeptides. The 4 were all
cytosolic and since they were separated by isoelectric focusing, they
are all charge variants. The molecular weights of the 3 other
polymorphic lymphocyte cytosol polypeptides were 40, 49, and 100 kD; see
174880. This stands in contrast to the restricted genetic variability in
fibroblast polypeptides (Walton et al., 1979; McConkey et al., 1979;
Giometti and Anderson, 1981).) Data on gene frequencies of allelic
variants were tabulated by Roychoudhury and Nei (1988).
ANIMAL MODEL
Chen et al. (2003) generated viable mice lacking Lpl. Lpl -/- mice
showed no Lpl expression in polymorphonuclear neutrophils (PMNs) and
macrophages and no aberrant expression of Pls1 (602734) or Pls3. Lpl -/-
mice were deficient in control of Staphylococcus aureus infection in
vivo and in vitro. Like Itgb2 (600065)-deficient PMNs, Lpl-deficient
PMNs were defective in adhesion-dependent respiratory burst due to
diminished Syk (600085) activation. However, unlike Itgb2-deficient
PMNs, they were able to adhere and spread normally. Lpl -/- bone
marrow-derived macrophages were deficient in adhesion-dependent
secretion of Il1b (147720). Chen et al. (2003) concluded that plastin
and Itgb2 interact in adhesion-dependent signaling.
*FIELD* SA
Haines (1986); Hamaguchi et al. (1981); Hamaguchi et al. (1982);
Hamaguchi et al. (1982); Klose and Feller (1981); Kondo and Hamaguchi
(1985); Kondo and Hamaguchi (1985); Kondo et al. (1985); O'Farrell
et al. (1977)
*FIELD* RF
1. Chen, H.; Mocsai, A.; Zhang, H.; Ding, R.-X.; Morisaki, J. H.;
White, M.; Rothfork, J. M.; Heiser, P.; Colucci-Guyon, E.; Lowell,
C. A.; Gresham, H. D.; Allen, P. M.; Brown, E. J.: Role for plastin
in host defense distinguishes integrin signaling from cell adhesion
and spreading. Immunity 19: 95-104, 2003.
2. Galiegue-Zouitina, S.; Quief, S.; Hildebrand, M.-P.; Denis, C.;
Detourmignies, L.; Lai, J.-L.; Kerckaert, J.-P.: Nonrandom fusion
of L-plastin (LCP1) and LAZ3 (BCL6) genes by t(3;13)(q27;q14) chromosome
translocation in two cases of B-cell non-Hodgkin lymphoma. Genes
Chromosomes Cancer 26: 97-105, 1999.
3. Giometti, C. S.; Anderson, N. L.: A variant of human nonmuscle
tropomyosin found in fibroblasts by using two-dimensional electrophoresis. J.
Biol. Chem. 256: 11840-11846, 1981.
4. Goldman, D.; O'Brien, S. J.; Lucas-Derse, S.; Dean, M.: Linkage
mapping of human polymorphic proteins identified by two-dimensional
electrophoresis. Genomics 11: 875-884, 1991.
5. Haines, J. L.: Error detected in published lod scores. Am. J.
Hum. Genet. 39: 544 only, 1986.
6. Hamaguchi, H.; Ohta, A.; Mukai, R.; Yabe, T.; Yamada, M.: Genetic
analysis of human lymphocyte proteins by two-dimensional gel electrophoresis:
1. Detection of genetic variant polypeptides in PHA-stimulated peripheral
blood lymphocyte. Hum. Genet. 59: 215-220, 1981.
7. Hamaguchi, H.; Yamada, M.; Noguchi, A.; Fujii, K.; Shibasaki, M.;
Mukai, R.; Yabe, T.; Kondo, I.: Genetic analysis of human lymphocyte
proteins by two-dimensional gel electrophoresis: 2. Genetic polymorphism
of lymphocyte cytosol 64K polypeptide. Hum. Genet. 60: 176-180,
1982.
8. Hamaguchi, H.; Yamada, M.; Shibasaki, M.; Kondo, I.: Genetic analysis
of human lymphocyte proteins by two-dimensional gel electrophoresis:
4. Genetic polymorphism of cytosol 100k polypeptide. Hum. Genet. 62:
148-151, 1982.
9. Hamaguchi, H.; Yamada, M.; Shibasaki, M.; Mukai, R.; Yabe, T.;
Kondo, I.: Genetic analysis of human lymphocyte proteins by two-dimensional
gel electrophoresis: 3. Frequent occurrence of genetic variants in
some abundant polypeptides of PHA-stimulated peripheral blood lymphocytes. Hum.
Genet. 62: 142-147, 1982.
10. Klose, J.: Protein mapping by combined isoelectric focusing and
electrophoresis of mouse tissue: a novel approach to testing for induced
point mutations in mammals. Humangenetik 26: 231-243, 1975.
11. Klose, J.; Feller, M.: Genetic variability of proteins from plasma
membranes and cytosols of mouse organs. Biochem. Genet. 19: 859-870,
1981.
12. Kondo, I.; Hamaguchi, H.: Evidence for the close linkage between
lymphocyte cytosol polypeptide with molecular weight of 64,000 (LCP1)
and esterase D. Am. J. Hum. Genet. 37: 1106-1111, 1985. Note: Erratum:
Am. J. Hum. Genet. 39: 544 only, 1986.
13. Kondo, I.; Hamaguchi, H.: Study of the linkage relationship between
LCP1 and ESD. (Abstract) Cytogenet. Cell Genet. 40: 672 only, 1985.
14. Kondo, I.; Ikeuchi, T.; Nishigaki, I.; Takita, H.; Fujiki, K.;
Takahashi, Y.; Hamaguchi, H.: Assignment of the gene for LCP1 on
chromosome 13. (Abstract) Cytogenet. Cell Genet. 40: 673 only, 1985.
15. Kondo, I.; Shin, K.; Honmura, S.; Nakajima, H.; Yamamura, E.;
Satoh, H.; Terauchi, M.; Usuki, Y.; Takita, H.; Hamaguchi, H.: A
case report of a patient with retinoblastoma and chromosome 13q deletion:
assignment of a new gene (gene for LCP1) on human chromosome 13. Hum.
Genet. 71: 263-266, 1985.
16. Lin, C.-S.; Aebersold, R. H.; Kent, S. B.; Varma, M.; Leavitt,
J.: Molecular cloning and characterization of plastin, a human leukocyte
protein expressed in transformed human fibroblasts. Molec. Cell.
Biol. 8: 4659-4668, 1988.
17. Lin, C.-S.; Chang, C.-H.; Huynh, T.: The murine L-plastin gene
promoter: identification and comparison with the human L-plastin gene
promoter. DNA Cell Biol. 16: 9-16, 1997.
18. Lin, C.-S.; Park, T.; Chen, Z. P.; Leavitt, J.: Human plastin
genes: comparative gene structure, chromosome location, and differential
expression in normal and neoplastic cells. J. Biol. Chem. 268: 2781-2792,
1993.
19. McConkey, E. H.; Taylor, B. J.; Phan, D.: Human heterozygosity:
a new estimate. Proc. Nat. Acad. Sci. 76: 6500-6504, 1979.
20. Murayama, N.; Tanaka, Y.; Hanyu, M.; Kobayashi, K.; Hamaguchi,
H.; Kondo, I.: Assignment of l-plastin to 13q14.3. (Abstract) Human
Genome Mapping Workshop 93 27 only, 1993.
21. O'Farrell, P. H.: High resolution two-dimensional electrophoresis
of proteins. J. Biol. Chem. 250: 4007-4021, 1975.
22. O'Farrell, P. Z.; Goodman, H. M.; O'Farrell, P. H.: High resolution
two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:
1133-1142, 1977.
23. Roychoudhury, A. K.; Nei, M.: Human Polymorphic Genes: World
Distribution. New York: Oxford Univ. Press (pub.) 1988.
24. Walton, K. E.; Steyer, D.; Gruenstein, E. I.: Genetic polymorphism
in normal human fibroblasts as analyzed by two-dimensional polyacrylamide
gel electrophoresis. J. Biol. Chem. 254: 7951-7960, 1979.
25. Zu, Y.; Kohno, M.; Kubota, I.; Nishida, E.; Hanaoka, M.; Namba,
Y.: Characterization of interleukin 2 stimulated 65-kilodalton phosphoprotein
in human T cells. Biochemistry 29: 1055-1062, 1990.
26. Zu, Y.; Shigesada, K.; Nishida, E.; Kubota, I.; Kohno, M.; Hanaoka,
M.; Namba, Y.: 65-kilodalton protein phosphorylated by interleukin
2 stimulation bears two putative actin-binding sites and two calcium-binding
sites. Biochemistry 29: 8319-8324, 1990.
*FIELD* CN
Paul J. Converse - updated: 5/16/2006
Rebekah S. Rasooly - updated: 5/19/1998
*FIELD* CD
Victor A. McKusick: 6/2/1986
*FIELD* ED
alopez: 11/12/2012
mgross: 6/1/2006
terry: 5/16/2006
mcapotos: 1/28/2000
terry: 11/3/1998
terry: 10/28/1998
psherman: 5/19/1998
carol: 4/23/1998
terry: 1/2/1997
terry: 5/12/1994
warfield: 3/1/1994
carol: 12/2/1993
supermim: 3/16/1992
carol: 12/5/1991
carol: 2/13/1991
*RECORD*
*FIELD* NO
153430
*FIELD* TI
*153430 LYMPHOCYTE CYTOSOLIC PROTEIN 1; LCP1
;;LYMPHOCYTE CYTOSOL POLYPEPTIDE, 64-KD; LC64P; CP64;;
read moreL-PLASTIN; LPL;;
PLASTIN 2; PLS2
*FIELD* TX
CLONING
Lin et al. (1988) isolated partial cDNAs encoding L-plastin and
T-plastin (PLS3; 300131) from a transformed human fibroblast cDNA
library. Northern blot analysis revealed that L-plastin is expressed as
a 3.7-kb mRNA in leukocytes, transformed fibroblasts, and a diverse
array of human tumor cell lines.
Zu et al. (1990) reported that L-plastin is identical to p65, an
interleukin-2 (IL2; 147680)-stimulated phosphoprotein found in human T
cells. Zu et al. (1990) isolated p65 cDNAs from a human T-lymphocyte
cDNA library. The predicted 627-amino acid p65 protein contains 2
EF-hand calcium-binding domains, a calmodulin-binding site, and 2 tandem
repeats of an actin-binding domain.
GENE STRUCTURE
Lin et al. (1993) reported that both the L-plastin and T-plastin genes
contain 16 exons and span approximately 90 kb. Lin et al. (1997) found
that the human and murine L-plastin promoters are highly homologous and
function equally well in either human or murine leukocytes.
MAPPING
Kondo et al. (1985) assigned the LCP1 gene to 13q14.1-q14.2 by the
deletion/dosage method. They studied a patient with trisomy 13 who had
1.5 times the normal amount of protein, a patient with retinoblastoma
and deletion of 13q12.3-q21.2 who had half the normal amount of protein,
and a patient with retinoblastoma and deletion of 13q14.1-q31.2 who had
lost the father's allele and had half the normal amount of protein
(Kondo et al., 1985). Close linkage to ESD (133280) was indicated by a
maximum lod score of 4.221 at zero recombination. As part of an
undertaking to map by genetic linkage human lymphocyte proteins that are
genetically polymorphic in isoelectric point, Goldman et al. (1991)
mapped the LCP1 gene to a site near the ESD locus on chromosome 13 by
studies in 9 families from the CEPH collection. The proteins in the
immortalized lymphocyte cell lines were analyzed by 2-dimensional
electrophoresis. Murayama et al. (1993) demonstrated that L-plastin and
LCP1 are identical and mapped the gene to 13q14.3.
CYTOGENETICS
The LAZ3 gene (BCL6; 109565) on 3q27 is nonrandomly disrupted in B-cell
non-Hodgkin lymphoma by chromosomal translocations. Galiegue-Zouitina et
al. (1999) identified the L-plastin gene as a novel LAZ3 partner in
chimeric transcripts resulting from a t(3;13)(q27;q14) translocation in
2 cases of B-cell lymphoma. As a consequence of the translocation, the
5-prime regulatory region of each gene was exchanged, creating both
LCP1-LAZ3 and reciprocal LAZ3-LCP1 fusion transcripts in one case, and
only an LCP1-LAZ3 fusion transcript in the other.
MOLECULAR GENETICS
Hamaguchi et al. (1982) described a genetic polymorphism of a major
human LCP of molecular weight 64,000, detected in PHA-stimulated
peripheral blood lymphocytes by high-resolution 2-dimensional
electrophoresis (O'Farrell, 1975; Klose, 1975). Three different
phenotypes determined by 2 common alleles at a single locus were found.
In Japanese, the frequency of the 2 alleles was 0.936 and 0.064,
respectively. The polypeptide was not detected in HeLa cells,
fibroblasts, red cells, serum, or cerebrum. Traces were found in liver,
kidney and skeletal muscle. (Hamaguchi et al. (1982) demonstrated
polymorphism in 3 others of about 100 polypeptides. The 4 were all
cytosolic and since they were separated by isoelectric focusing, they
are all charge variants. The molecular weights of the 3 other
polymorphic lymphocyte cytosol polypeptides were 40, 49, and 100 kD; see
174880. This stands in contrast to the restricted genetic variability in
fibroblast polypeptides (Walton et al., 1979; McConkey et al., 1979;
Giometti and Anderson, 1981).) Data on gene frequencies of allelic
variants were tabulated by Roychoudhury and Nei (1988).
ANIMAL MODEL
Chen et al. (2003) generated viable mice lacking Lpl. Lpl -/- mice
showed no Lpl expression in polymorphonuclear neutrophils (PMNs) and
macrophages and no aberrant expression of Pls1 (602734) or Pls3. Lpl -/-
mice were deficient in control of Staphylococcus aureus infection in
vivo and in vitro. Like Itgb2 (600065)-deficient PMNs, Lpl-deficient
PMNs were defective in adhesion-dependent respiratory burst due to
diminished Syk (600085) activation. However, unlike Itgb2-deficient
PMNs, they were able to adhere and spread normally. Lpl -/- bone
marrow-derived macrophages were deficient in adhesion-dependent
secretion of Il1b (147720). Chen et al. (2003) concluded that plastin
and Itgb2 interact in adhesion-dependent signaling.
*FIELD* SA
Haines (1986); Hamaguchi et al. (1981); Hamaguchi et al. (1982);
Hamaguchi et al. (1982); Klose and Feller (1981); Kondo and Hamaguchi
(1985); Kondo and Hamaguchi (1985); Kondo et al. (1985); O'Farrell
et al. (1977)
*FIELD* RF
1. Chen, H.; Mocsai, A.; Zhang, H.; Ding, R.-X.; Morisaki, J. H.;
White, M.; Rothfork, J. M.; Heiser, P.; Colucci-Guyon, E.; Lowell,
C. A.; Gresham, H. D.; Allen, P. M.; Brown, E. J.: Role for plastin
in host defense distinguishes integrin signaling from cell adhesion
and spreading. Immunity 19: 95-104, 2003.
2. Galiegue-Zouitina, S.; Quief, S.; Hildebrand, M.-P.; Denis, C.;
Detourmignies, L.; Lai, J.-L.; Kerckaert, J.-P.: Nonrandom fusion
of L-plastin (LCP1) and LAZ3 (BCL6) genes by t(3;13)(q27;q14) chromosome
translocation in two cases of B-cell non-Hodgkin lymphoma. Genes
Chromosomes Cancer 26: 97-105, 1999.
3. Giometti, C. S.; Anderson, N. L.: A variant of human nonmuscle
tropomyosin found in fibroblasts by using two-dimensional electrophoresis. J.
Biol. Chem. 256: 11840-11846, 1981.
4. Goldman, D.; O'Brien, S. J.; Lucas-Derse, S.; Dean, M.: Linkage
mapping of human polymorphic proteins identified by two-dimensional
electrophoresis. Genomics 11: 875-884, 1991.
5. Haines, J. L.: Error detected in published lod scores. Am. J.
Hum. Genet. 39: 544 only, 1986.
6. Hamaguchi, H.; Ohta, A.; Mukai, R.; Yabe, T.; Yamada, M.: Genetic
analysis of human lymphocyte proteins by two-dimensional gel electrophoresis:
1. Detection of genetic variant polypeptides in PHA-stimulated peripheral
blood lymphocyte. Hum. Genet. 59: 215-220, 1981.
7. Hamaguchi, H.; Yamada, M.; Noguchi, A.; Fujii, K.; Shibasaki, M.;
Mukai, R.; Yabe, T.; Kondo, I.: Genetic analysis of human lymphocyte
proteins by two-dimensional gel electrophoresis: 2. Genetic polymorphism
of lymphocyte cytosol 64K polypeptide. Hum. Genet. 60: 176-180,
1982.
8. Hamaguchi, H.; Yamada, M.; Shibasaki, M.; Kondo, I.: Genetic analysis
of human lymphocyte proteins by two-dimensional gel electrophoresis:
4. Genetic polymorphism of cytosol 100k polypeptide. Hum. Genet. 62:
148-151, 1982.
9. Hamaguchi, H.; Yamada, M.; Shibasaki, M.; Mukai, R.; Yabe, T.;
Kondo, I.: Genetic analysis of human lymphocyte proteins by two-dimensional
gel electrophoresis: 3. Frequent occurrence of genetic variants in
some abundant polypeptides of PHA-stimulated peripheral blood lymphocytes. Hum.
Genet. 62: 142-147, 1982.
10. Klose, J.: Protein mapping by combined isoelectric focusing and
electrophoresis of mouse tissue: a novel approach to testing for induced
point mutations in mammals. Humangenetik 26: 231-243, 1975.
11. Klose, J.; Feller, M.: Genetic variability of proteins from plasma
membranes and cytosols of mouse organs. Biochem. Genet. 19: 859-870,
1981.
12. Kondo, I.; Hamaguchi, H.: Evidence for the close linkage between
lymphocyte cytosol polypeptide with molecular weight of 64,000 (LCP1)
and esterase D. Am. J. Hum. Genet. 37: 1106-1111, 1985. Note: Erratum:
Am. J. Hum. Genet. 39: 544 only, 1986.
13. Kondo, I.; Hamaguchi, H.: Study of the linkage relationship between
LCP1 and ESD. (Abstract) Cytogenet. Cell Genet. 40: 672 only, 1985.
14. Kondo, I.; Ikeuchi, T.; Nishigaki, I.; Takita, H.; Fujiki, K.;
Takahashi, Y.; Hamaguchi, H.: Assignment of the gene for LCP1 on
chromosome 13. (Abstract) Cytogenet. Cell Genet. 40: 673 only, 1985.
15. Kondo, I.; Shin, K.; Honmura, S.; Nakajima, H.; Yamamura, E.;
Satoh, H.; Terauchi, M.; Usuki, Y.; Takita, H.; Hamaguchi, H.: A
case report of a patient with retinoblastoma and chromosome 13q deletion:
assignment of a new gene (gene for LCP1) on human chromosome 13. Hum.
Genet. 71: 263-266, 1985.
16. Lin, C.-S.; Aebersold, R. H.; Kent, S. B.; Varma, M.; Leavitt,
J.: Molecular cloning and characterization of plastin, a human leukocyte
protein expressed in transformed human fibroblasts. Molec. Cell.
Biol. 8: 4659-4668, 1988.
17. Lin, C.-S.; Chang, C.-H.; Huynh, T.: The murine L-plastin gene
promoter: identification and comparison with the human L-plastin gene
promoter. DNA Cell Biol. 16: 9-16, 1997.
18. Lin, C.-S.; Park, T.; Chen, Z. P.; Leavitt, J.: Human plastin
genes: comparative gene structure, chromosome location, and differential
expression in normal and neoplastic cells. J. Biol. Chem. 268: 2781-2792,
1993.
19. McConkey, E. H.; Taylor, B. J.; Phan, D.: Human heterozygosity:
a new estimate. Proc. Nat. Acad. Sci. 76: 6500-6504, 1979.
20. Murayama, N.; Tanaka, Y.; Hanyu, M.; Kobayashi, K.; Hamaguchi,
H.; Kondo, I.: Assignment of l-plastin to 13q14.3. (Abstract) Human
Genome Mapping Workshop 93 27 only, 1993.
21. O'Farrell, P. H.: High resolution two-dimensional electrophoresis
of proteins. J. Biol. Chem. 250: 4007-4021, 1975.
22. O'Farrell, P. Z.; Goodman, H. M.; O'Farrell, P. H.: High resolution
two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:
1133-1142, 1977.
23. Roychoudhury, A. K.; Nei, M.: Human Polymorphic Genes: World
Distribution. New York: Oxford Univ. Press (pub.) 1988.
24. Walton, K. E.; Steyer, D.; Gruenstein, E. I.: Genetic polymorphism
in normal human fibroblasts as analyzed by two-dimensional polyacrylamide
gel electrophoresis. J. Biol. Chem. 254: 7951-7960, 1979.
25. Zu, Y.; Kohno, M.; Kubota, I.; Nishida, E.; Hanaoka, M.; Namba,
Y.: Characterization of interleukin 2 stimulated 65-kilodalton phosphoprotein
in human T cells. Biochemistry 29: 1055-1062, 1990.
26. Zu, Y.; Shigesada, K.; Nishida, E.; Kubota, I.; Kohno, M.; Hanaoka,
M.; Namba, Y.: 65-kilodalton protein phosphorylated by interleukin
2 stimulation bears two putative actin-binding sites and two calcium-binding
sites. Biochemistry 29: 8319-8324, 1990.
*FIELD* CN
Paul J. Converse - updated: 5/16/2006
Rebekah S. Rasooly - updated: 5/19/1998
*FIELD* CD
Victor A. McKusick: 6/2/1986
*FIELD* ED
alopez: 11/12/2012
mgross: 6/1/2006
terry: 5/16/2006
mcapotos: 1/28/2000
terry: 11/3/1998
terry: 10/28/1998
psherman: 5/19/1998
carol: 4/23/1998
terry: 1/2/1997
terry: 5/12/1994
warfield: 3/1/1994
carol: 12/2/1993
supermim: 3/16/1992
carol: 12/5/1991
carol: 2/13/1991