Full text data of SRI
SRI
[Confidence: low (only semi-automatic identification from reviews)]
Sorcin (22 kDa protein; CP-22; CP22; V19)
Sorcin (22 kDa protein; CP-22; CP22; V19)
UniProt
P30626
ID SORCN_HUMAN Reviewed; 198 AA.
AC P30626; A8MTH6; D6W5Q0;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-APR-1993, sequence version 1.
DT 22-JAN-2014, entry version 146.
DE RecName: Full=Sorcin;
DE AltName: Full=22 kDa protein;
DE AltName: Full=CP-22;
DE Short=CP22;
DE AltName: Full=V19;
GN Name=SRI;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=7873602; DOI=10.1016/0167-4781(94)00206-I;
RA Wang S.L., Tam M.F., Ho Y.S., Pai S.H., Kao M.C.;
RT "Isolation and molecular cloning of human sorcin a calcium-binding
RT protein in vincristine-resistant HOB1 lymphoma cells.";
RL Biochim. Biophys. Acta 1260:285-293(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8561500; DOI=10.1006/abbi.1996.0027;
RA Lee W.P.;
RT "Purification, cDNA cloning, and expression of human sorcin in
RT vincristine-resistant HOB1 lymphoma cell lines.";
RL Arch. Biochem. Biophys. 325:217-226(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Ascites;
RX PubMed=16341674; DOI=10.1007/s00335-005-0075-2;
RA Oh J.H., Yang J.O., Hahn Y., Kim M.R., Byun S.S., Jeon Y.J., Kim J.M.,
RA Song K.S., Noh S.M., Kim S., Yoo H.S., Kim Y.S., Kim N.S.;
RT "Transcriptome analysis of human gastric cancer.";
RL Mamm. Genome 16:942-954(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Sugimoto Y., Asami N., Okochi E., Ogura M., Tsuruo T.;
RT "Isolation of the cDNA clone for human CP22 overexpressed in
RT multidrug-resistant cell lines.";
RL Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R.,
RA Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H.,
RA Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A.,
RA Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J.,
RA Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A.,
RA Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S.,
RA Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M.,
RA Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C.,
RA Latreille P., Miller N., Johnson D., Murray J., Woessner J.P.,
RA Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J.,
RA Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L.,
RA Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R.,
RA Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K.,
RA Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S.,
RA Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M.,
RA Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R.,
RA Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D.,
RA Waterston R.H., Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP PROTEIN SEQUENCE OF 57-116; 128-135 AND 154-165, AND MASS
RP SPECTROMETRY.
RC TISSUE=Fetal brain cortex;
RA Lubec G., Chen W.-Q., Sun Y.;
RL Submitted (DEC-2008) to UniProtKB.
RN [9]
RP PROTEIN SEQUENCE OF 128-135 AND 154-165.
RC TISSUE=Keratinocyte;
RX PubMed=1286667; DOI=10.1002/elps.11501301199;
RA Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E.,
RA Vandekerckhove J.;
RT "Microsequences of 145 proteins recorded in the two-dimensional gel
RT protein database of normal human epidermal keratinocytes.";
RL Electrophoresis 13:960-969(1992).
RN [10]
RP INTERACTION WITH GCA.
RX PubMed=12804766; DOI=10.1016/S0014-5793(03)00518-0;
RA Hansen C., Tarabykina S., la Cour J.M., Lollike K., Berchtold M.W.;
RT "The PEF family proteins sorcin and grancalcin interact in vivo and in
RT vitro.";
RL FEBS Lett. 545:151-154(2003).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX PubMed=11714909; DOI=10.1110/ps.ps.36701;
RA Xie X., Dwyer M.D., Swenson L., Parker M.H., Botfield M.C.;
RT "Crystal structure of calcium-free human sorcin: a member of the
RT penta-EF-hand protein family.";
RL Protein Sci. 10:2419-2425(2001).
RN [13]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF MUTANT LEU-112, SUBUNIT,
RP FUNCTION, CALCIUM-BINDING, AND INTERACTION WITH RYR2 AND ANXA7.
RX PubMed=17699613; DOI=10.1096/fj.07-8988com;
RA Franceschini S., Ilari A., Verzili D., Zamparelli C., Antaramian A.,
RA Rueda A., Valdivia H.H., Chiancone E., Colotti G.;
RT "Molecular basis for the impaired function of the natural F112L sorcin
RT mutant: X-ray crystal structure, calcium affinity, and interaction
RT with annexin VII and the ryanodine receptor.";
RL FASEB J. 22:295-306(2008).
CC -!- FUNCTION: Calcium-binding protein that modulates excitation-
CC contraction coupling in the heart. Contributes to calcium
CC homeostasis in the heart sarcoplasmic reticulum. Modulates the
CC activity of RYR2 calcium channels.
CC -!- SUBUNIT: Homodimer. Interacts with GCA, RYR2 and ANXA7.
CC -!- INTERACTION:
CC P40763:STAT3; NbExp=2; IntAct=EBI-750459, EBI-518675;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Sarcoplasmic reticulum membrane;
CC Peripheral membrane protein; Cytoplasmic side. Note=Relocates to
CC the sarcoplasmic reticulum membrane in response to elevated
CC calcium levels.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P30626-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P30626-2; Sequence=VSP_046277;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Detected in cardiac myocytes.
CC -!- MISCELLANEOUS: This protein is encoded by an amplified gene in
CC multidrug-resistant cells.
CC -!- MISCELLANEOUS: This protein has been shown to bind calcium with
CC high affinity.
CC -!- SIMILARITY: Contains 4 EF-hand domains.
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DR EMBL; L12387; AAA92155.1; -; mRNA.
DR EMBL; BM739144; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; M32886; AAA60588.1; -; mRNA.
DR EMBL; AC003991; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC005075; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471091; EAW76909.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76910.1; -; Genomic_DNA.
DR EMBL; BC011025; AAH11025.1; -; mRNA.
DR PIR; S52094; S52094.
DR RefSeq; NP_003121.1; NM_003130.3.
DR RefSeq; NP_944490.1; NM_198901.1.
DR UniGene; Hs.489040; -.
DR PDB; 1JUO; X-ray; 2.20 A; A/B=1-198.
DR PDB; 2JC2; X-ray; 2.50 A; A/B/C/D=1-198.
DR PDBsum; 1JUO; -.
DR PDBsum; 2JC2; -.
DR ProteinModelPortal; P30626; -.
DR SMR; P30626; 27-198.
DR IntAct; P30626; 10.
DR MINT; MINT-4999795; -.
DR STRING; 9606.ENSP00000265729; -.
DR PhosphoSite; P30626; -.
DR DMDM; 267021; -.
DR OGP; P30626; -.
DR PaxDb; P30626; -.
DR PRIDE; P30626; -.
DR Ensembl; ENST00000265729; ENSP00000265729; ENSG00000075142.
DR Ensembl; ENST00000394641; ENSP00000378137; ENSG00000075142.
DR GeneID; 6717; -.
DR KEGG; hsa:6717; -.
DR UCSC; uc003ujr.1; human.
DR CTD; 6717; -.
DR GeneCards; GC07M087834; -.
DR HGNC; HGNC:11292; SRI.
DR MIM; 182520; gene.
DR neXtProt; NX_P30626; -.
DR PharmGKB; PA36117; -.
DR eggNOG; NOG298587; -.
DR HOGENOM; HOG000231982; -.
DR HOVERGEN; HBG004492; -.
DR OMA; GQQGVVN; -.
DR OrthoDB; EOG7RV9FM; -.
DR PhylomeDB; P30626; -.
DR ChiTaRS; SRI; human.
DR EvolutionaryTrace; P30626; -.
DR GeneWiki; SRI_(gene); -.
DR GenomeRNAi; 6717; -.
DR NextBio; 26200; -.
DR PRO; PR:P30626; -.
DR ArrayExpress; P30626; -.
DR Bgee; P30626; -.
DR CleanEx; HS_SRI; -.
DR Genevestigator; P30626; -.
DR GO; GO:0005829; C:cytosol; IDA:BHF-UCL.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:BHF-UCL.
DR GO; GO:0016529; C:sarcoplasmic reticulum; TAS:BHF-UCL.
DR GO; GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030315; C:T-tubule; TAS:BHF-UCL.
DR GO; GO:0031982; C:vesicle; IEA:Ensembl.
DR GO; GO:0030018; C:Z disc; IDA:BHF-UCL.
DR GO; GO:0005246; F:calcium channel regulator activity; TAS:ProtInc.
DR GO; GO:0005509; F:calcium ion binding; IDA:BHF-UCL.
DR GO; GO:0044325; F:ion channel binding; TAS:BHF-UCL.
DR GO; GO:0005102; F:receptor binding; TAS:ProtInc.
DR GO; GO:0006816; P:calcium ion transport; IEA:Ensembl.
DR GO; GO:0042994; P:cytoplasmic sequestering of transcription factor; IEA:Ensembl.
DR GO; GO:0007507; P:heart development; TAS:ProtInc.
DR GO; GO:0006880; P:intracellular sequestering of iron ion; TAS:ProtInc.
DR GO; GO:0007517; P:muscle organ development; TAS:ProtInc.
DR GO; GO:0010459; P:negative regulation of heart rate; IMP:BHF-UCL.
DR GO; GO:0060315; P:negative regulation of ryanodine-sensitive calcium-release channel activity; IDA:BHF-UCL.
DR GO; GO:2000678; P:negative regulation of transcription regulatory region DNA binding; IEA:Ensembl.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Ensembl.
DR GO; GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IEA:Ensembl.
DR GO; GO:0001508; P:regulation of action potential; TAS:ProtInc.
DR GO; GO:0086004; P:regulation of cardiac muscle cell contraction; IMP:BHF-UCL.
DR GO; GO:1901844; P:regulation of cell communication by electrical coupling involved in cardiac conduction; IMP:BHF-UCL.
DR GO; GO:1901841; P:regulation of high voltage-gated calcium channel activity; IMP:BHF-UCL.
DR GO; GO:1901077; P:regulation of relaxation of muscle; IMP:BHF-UCL.
DR GO; GO:0010880; P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; TAS:BHF-UCL.
DR GO; GO:0007165; P:signal transduction; TAS:UniProtKB.
DR GO; GO:0006810; P:transport; TAS:ProtInc.
DR Gene3D; 1.10.238.10; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Calcium; Complete proteome;
KW Cytoplasm; Direct protein sequencing; Membrane; Metal-binding;
KW Reference proteome; Repeat; Sarcoplasmic reticulum.
FT CHAIN 1 198 Sorcin.
FT /FTId=PRO_0000073725.
FT DOMAIN 29 64 EF-hand 1.
FT DOMAIN 70 103 EF-hand 2.
FT DOMAIN 100 135 EF-hand 3.
FT DOMAIN 134 169 EF-hand 4.
FT CA_BIND 83 94 1 (Probable).
FT CA_BIND 113 124 2 (Probable).
FT VAR_SEQ 1 17 MAYPGHPGAGGGYYPGG -> MQ (in isoform 2).
FT /FTId=VSP_046277.
FT MUTAGEN 112 112 F->L: Reduces affinity for calcium 5-
FT fold.
FT HELIX 36 43
FT TURN 44 47
FT HELIX 51 61
FT TURN 62 64
FT STRAND 65 68
FT HELIX 72 82
FT STRAND 84 86
FT HELIX 92 110
FT STRAND 118 120
FT HELIX 122 131
FT HELIX 138 147
FT STRAND 150 155
FT HELIX 156 175
FT HELIX 178 180
FT STRAND 182 187
FT HELIX 188 195
SQ SEQUENCE 198 AA; 21676 MW; A4829C7650A5E3FA CRC64;
MAYPGHPGAG GGYYPGGYGG APGGPAFPGQ TQDPLYGYFA AVAGQDGQID ADELQRCLTQ
SGIAGGYKPF NLETCRLMVS MLDRDMSGTM GFNEFKELWA VLNGWRQHFI SFDTDRSGTV
DPQELQKALT TMGFRLSPQA VNSIAKRYST NGKITFDDYI ACCVKLRALT DSFRRRDTAQ
QGVVNFPYDD FIQCVMSV
//
ID SORCN_HUMAN Reviewed; 198 AA.
AC P30626; A8MTH6; D6W5Q0;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-APR-1993, sequence version 1.
DT 22-JAN-2014, entry version 146.
DE RecName: Full=Sorcin;
DE AltName: Full=22 kDa protein;
DE AltName: Full=CP-22;
DE Short=CP22;
DE AltName: Full=V19;
GN Name=SRI;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=7873602; DOI=10.1016/0167-4781(94)00206-I;
RA Wang S.L., Tam M.F., Ho Y.S., Pai S.H., Kao M.C.;
RT "Isolation and molecular cloning of human sorcin a calcium-binding
RT protein in vincristine-resistant HOB1 lymphoma cells.";
RL Biochim. Biophys. Acta 1260:285-293(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8561500; DOI=10.1006/abbi.1996.0027;
RA Lee W.P.;
RT "Purification, cDNA cloning, and expression of human sorcin in
RT vincristine-resistant HOB1 lymphoma cell lines.";
RL Arch. Biochem. Biophys. 325:217-226(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Ascites;
RX PubMed=16341674; DOI=10.1007/s00335-005-0075-2;
RA Oh J.H., Yang J.O., Hahn Y., Kim M.R., Byun S.S., Jeon Y.J., Kim J.M.,
RA Song K.S., Noh S.M., Kim S., Yoo H.S., Kim Y.S., Kim N.S.;
RT "Transcriptome analysis of human gastric cancer.";
RL Mamm. Genome 16:942-954(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Sugimoto Y., Asami N., Okochi E., Ogura M., Tsuruo T.;
RT "Isolation of the cDNA clone for human CP22 overexpressed in
RT multidrug-resistant cell lines.";
RL Submitted (MAR-1990) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R.,
RA Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H.,
RA Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A.,
RA Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J.,
RA Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A.,
RA Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S.,
RA Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M.,
RA Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C.,
RA Latreille P., Miller N., Johnson D., Murray J., Woessner J.P.,
RA Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J.,
RA Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L.,
RA Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R.,
RA Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K.,
RA Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S.,
RA Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M.,
RA Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R.,
RA Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D.,
RA Waterston R.H., Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP PROTEIN SEQUENCE OF 57-116; 128-135 AND 154-165, AND MASS
RP SPECTROMETRY.
RC TISSUE=Fetal brain cortex;
RA Lubec G., Chen W.-Q., Sun Y.;
RL Submitted (DEC-2008) to UniProtKB.
RN [9]
RP PROTEIN SEQUENCE OF 128-135 AND 154-165.
RC TISSUE=Keratinocyte;
RX PubMed=1286667; DOI=10.1002/elps.11501301199;
RA Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E.,
RA Vandekerckhove J.;
RT "Microsequences of 145 proteins recorded in the two-dimensional gel
RT protein database of normal human epidermal keratinocytes.";
RL Electrophoresis 13:960-969(1992).
RN [10]
RP INTERACTION WITH GCA.
RX PubMed=12804766; DOI=10.1016/S0014-5793(03)00518-0;
RA Hansen C., Tarabykina S., la Cour J.M., Lollike K., Berchtold M.W.;
RT "The PEF family proteins sorcin and grancalcin interact in vivo and in
RT vitro.";
RL FEBS Lett. 545:151-154(2003).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
RX PubMed=11714909; DOI=10.1110/ps.ps.36701;
RA Xie X., Dwyer M.D., Swenson L., Parker M.H., Botfield M.C.;
RT "Crystal structure of calcium-free human sorcin: a member of the
RT penta-EF-hand protein family.";
RL Protein Sci. 10:2419-2425(2001).
RN [13]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF MUTANT LEU-112, SUBUNIT,
RP FUNCTION, CALCIUM-BINDING, AND INTERACTION WITH RYR2 AND ANXA7.
RX PubMed=17699613; DOI=10.1096/fj.07-8988com;
RA Franceschini S., Ilari A., Verzili D., Zamparelli C., Antaramian A.,
RA Rueda A., Valdivia H.H., Chiancone E., Colotti G.;
RT "Molecular basis for the impaired function of the natural F112L sorcin
RT mutant: X-ray crystal structure, calcium affinity, and interaction
RT with annexin VII and the ryanodine receptor.";
RL FASEB J. 22:295-306(2008).
CC -!- FUNCTION: Calcium-binding protein that modulates excitation-
CC contraction coupling in the heart. Contributes to calcium
CC homeostasis in the heart sarcoplasmic reticulum. Modulates the
CC activity of RYR2 calcium channels.
CC -!- SUBUNIT: Homodimer. Interacts with GCA, RYR2 and ANXA7.
CC -!- INTERACTION:
CC P40763:STAT3; NbExp=2; IntAct=EBI-750459, EBI-518675;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Sarcoplasmic reticulum membrane;
CC Peripheral membrane protein; Cytoplasmic side. Note=Relocates to
CC the sarcoplasmic reticulum membrane in response to elevated
CC calcium levels.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P30626-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P30626-2; Sequence=VSP_046277;
CC Note=No experimental confirmation available;
CC -!- TISSUE SPECIFICITY: Detected in cardiac myocytes.
CC -!- MISCELLANEOUS: This protein is encoded by an amplified gene in
CC multidrug-resistant cells.
CC -!- MISCELLANEOUS: This protein has been shown to bind calcium with
CC high affinity.
CC -!- SIMILARITY: Contains 4 EF-hand domains.
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DR EMBL; L12387; AAA92155.1; -; mRNA.
DR EMBL; BM739144; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; M32886; AAA60588.1; -; mRNA.
DR EMBL; AC003991; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC005075; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471091; EAW76909.1; -; Genomic_DNA.
DR EMBL; CH471091; EAW76910.1; -; Genomic_DNA.
DR EMBL; BC011025; AAH11025.1; -; mRNA.
DR PIR; S52094; S52094.
DR RefSeq; NP_003121.1; NM_003130.3.
DR RefSeq; NP_944490.1; NM_198901.1.
DR UniGene; Hs.489040; -.
DR PDB; 1JUO; X-ray; 2.20 A; A/B=1-198.
DR PDB; 2JC2; X-ray; 2.50 A; A/B/C/D=1-198.
DR PDBsum; 1JUO; -.
DR PDBsum; 2JC2; -.
DR ProteinModelPortal; P30626; -.
DR SMR; P30626; 27-198.
DR IntAct; P30626; 10.
DR MINT; MINT-4999795; -.
DR STRING; 9606.ENSP00000265729; -.
DR PhosphoSite; P30626; -.
DR DMDM; 267021; -.
DR OGP; P30626; -.
DR PaxDb; P30626; -.
DR PRIDE; P30626; -.
DR Ensembl; ENST00000265729; ENSP00000265729; ENSG00000075142.
DR Ensembl; ENST00000394641; ENSP00000378137; ENSG00000075142.
DR GeneID; 6717; -.
DR KEGG; hsa:6717; -.
DR UCSC; uc003ujr.1; human.
DR CTD; 6717; -.
DR GeneCards; GC07M087834; -.
DR HGNC; HGNC:11292; SRI.
DR MIM; 182520; gene.
DR neXtProt; NX_P30626; -.
DR PharmGKB; PA36117; -.
DR eggNOG; NOG298587; -.
DR HOGENOM; HOG000231982; -.
DR HOVERGEN; HBG004492; -.
DR OMA; GQQGVVN; -.
DR OrthoDB; EOG7RV9FM; -.
DR PhylomeDB; P30626; -.
DR ChiTaRS; SRI; human.
DR EvolutionaryTrace; P30626; -.
DR GeneWiki; SRI_(gene); -.
DR GenomeRNAi; 6717; -.
DR NextBio; 26200; -.
DR PRO; PR:P30626; -.
DR ArrayExpress; P30626; -.
DR Bgee; P30626; -.
DR CleanEx; HS_SRI; -.
DR Genevestigator; P30626; -.
DR GO; GO:0005829; C:cytosol; IDA:BHF-UCL.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:BHF-UCL.
DR GO; GO:0016529; C:sarcoplasmic reticulum; TAS:BHF-UCL.
DR GO; GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030315; C:T-tubule; TAS:BHF-UCL.
DR GO; GO:0031982; C:vesicle; IEA:Ensembl.
DR GO; GO:0030018; C:Z disc; IDA:BHF-UCL.
DR GO; GO:0005246; F:calcium channel regulator activity; TAS:ProtInc.
DR GO; GO:0005509; F:calcium ion binding; IDA:BHF-UCL.
DR GO; GO:0044325; F:ion channel binding; TAS:BHF-UCL.
DR GO; GO:0005102; F:receptor binding; TAS:ProtInc.
DR GO; GO:0006816; P:calcium ion transport; IEA:Ensembl.
DR GO; GO:0042994; P:cytoplasmic sequestering of transcription factor; IEA:Ensembl.
DR GO; GO:0007507; P:heart development; TAS:ProtInc.
DR GO; GO:0006880; P:intracellular sequestering of iron ion; TAS:ProtInc.
DR GO; GO:0007517; P:muscle organ development; TAS:ProtInc.
DR GO; GO:0010459; P:negative regulation of heart rate; IMP:BHF-UCL.
DR GO; GO:0060315; P:negative regulation of ryanodine-sensitive calcium-release channel activity; IDA:BHF-UCL.
DR GO; GO:2000678; P:negative regulation of transcription regulatory region DNA binding; IEA:Ensembl.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Ensembl.
DR GO; GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IEA:Ensembl.
DR GO; GO:0001508; P:regulation of action potential; TAS:ProtInc.
DR GO; GO:0086004; P:regulation of cardiac muscle cell contraction; IMP:BHF-UCL.
DR GO; GO:1901844; P:regulation of cell communication by electrical coupling involved in cardiac conduction; IMP:BHF-UCL.
DR GO; GO:1901841; P:regulation of high voltage-gated calcium channel activity; IMP:BHF-UCL.
DR GO; GO:1901077; P:regulation of relaxation of muscle; IMP:BHF-UCL.
DR GO; GO:0010880; P:regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; TAS:BHF-UCL.
DR GO; GO:0007165; P:signal transduction; TAS:UniProtKB.
DR GO; GO:0006810; P:transport; TAS:ProtInc.
DR Gene3D; 1.10.238.10; -; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Calcium; Complete proteome;
KW Cytoplasm; Direct protein sequencing; Membrane; Metal-binding;
KW Reference proteome; Repeat; Sarcoplasmic reticulum.
FT CHAIN 1 198 Sorcin.
FT /FTId=PRO_0000073725.
FT DOMAIN 29 64 EF-hand 1.
FT DOMAIN 70 103 EF-hand 2.
FT DOMAIN 100 135 EF-hand 3.
FT DOMAIN 134 169 EF-hand 4.
FT CA_BIND 83 94 1 (Probable).
FT CA_BIND 113 124 2 (Probable).
FT VAR_SEQ 1 17 MAYPGHPGAGGGYYPGG -> MQ (in isoform 2).
FT /FTId=VSP_046277.
FT MUTAGEN 112 112 F->L: Reduces affinity for calcium 5-
FT fold.
FT HELIX 36 43
FT TURN 44 47
FT HELIX 51 61
FT TURN 62 64
FT STRAND 65 68
FT HELIX 72 82
FT STRAND 84 86
FT HELIX 92 110
FT STRAND 118 120
FT HELIX 122 131
FT HELIX 138 147
FT STRAND 150 155
FT HELIX 156 175
FT HELIX 178 180
FT STRAND 182 187
FT HELIX 188 195
SQ SEQUENCE 198 AA; 21676 MW; A4829C7650A5E3FA CRC64;
MAYPGHPGAG GGYYPGGYGG APGGPAFPGQ TQDPLYGYFA AVAGQDGQID ADELQRCLTQ
SGIAGGYKPF NLETCRLMVS MLDRDMSGTM GFNEFKELWA VLNGWRQHFI SFDTDRSGTV
DPQELQKALT TMGFRLSPQA VNSIAKRYST NGKITFDDYI ACCVKLRALT DSFRRRDTAQ
QGVVNFPYDD FIQCVMSV
//
MIM
182520
*RECORD*
*FIELD* NO
182520
*FIELD* TI
*182520 SORCIN; SRI
;;MULTIDRUG-RESISTANCE COMPLEX, CLASS 4;;
MDR COMPLEX, CLASS 4
read more*FIELD* TX
Van der Bliek et al. (1988) showed that the human homolog of the hamster
sorcin gene resides on chromosome 7 like the P-glycoprotein-encoding
genes (MDR1, 171050; MDR3, 171060). (The sorcin gene was assigned by
Southern analysis of DNA from somatic cell hybrids.) Furthermore, gene
classes designated 4, 5, and 6 were found to be coamplified with MDR1
and MDR3 in a human ovarian carcinoma cell line, which strongly
suggested that the overall structure of the human MDR domain is the same
as that in the hamster and mouse in which the domain which is amplified
to result in multidrug resistance (MDR) comprises not only genes that
encode P-glycoproteins, but also at least 5 unrelated genes, 1 of which
encodes the calcium-binding protein sorcin. Sorcin, coded by the class 4
gene, is a small cytosolic protein that is overproduced in many MDR
cells. It has a sequence similar to the regulatory light-chain of
calpain (114170), including calcium-binding loops of the type found in
calmodulin (114180). Van der Bliek et al. (1988) also found that a
sorcin-related sequence segregated with human chromosome 4. The total
area encompassed by the MDR1, MDR3, and classes 4-6 genes may be as much
as 1500 kb.
Wang et al. (1995) found that the 22-kD sorcin protein was overexpressed
in HOB1/VCR-1.0 cells, a multidrug-resistant human B immunoblastic
lymphoma cell line. By PCR with primers based on the sequence of hamster
sorcin, the authors recovered a partial human sorcin cDNA. Using the
partial cDNA to screen a HOB1/VCR-1.0 library, they isolated cDNAs
corresponding to the entire sorcin coding region. The predicted
198-amino acid human protein shares 95% sequence identity with hamster
sorcin. Human sorcin contains 4 EF-hand calcium-binding motifs. Purified
protein bound calcium in vitro. Southern and Northern blot analysis
revealed that the sorcin gene is amplified and overexpressed in
HOB1/VCR-1.0 cells. No expression was detected in parental HOB1 cells.
Independently, Lee (1996) cloned the human sorcin gene.
In both heart and skeletal muscle, the geometry and close spatial
relationship between sarcolemmal L-type calcium channels and
sarcoplasmic reticulum ryanodine receptors (RyRs, see 180903) is
critical in determining the time course of calcium release and
excitation-contraction coupling. Meyers et al. (1998) stated that sorcin
is 1 of a group of modulators of RyR gating. The authors demonstrated
that sorcin also associates with cardiac and skeletal muscle L-type
calcium channels specifically within the cytoplasmically oriented
C-terminal region of the alpha-1 subunits. See 114204. Meyers et al.
(1998) suggested that sorcin is a candidate regulator of interchannel
communication during excitation-contraction coupling in heart and
skeletal muscle.
*FIELD* RF
1. Lee, W. P.: Purification, cDNA cloning, and expression of human
sorcin in vincristine-resistant HOB1 lymphoma cell lines. Arch. Biochem.
Biophys. 325: 217-226, 1996.
2. Meyers, M. B.; Puri, T. S.; Chien, A. J.; Gao, T.; Hsu, P.-H.;
Hosey, M. M.; Fishman, G. I.: Sorcin associates with the pore-forming
subunit of voltage-dependent L-type Ca(2+) channels. J. Biol. Chem. 273:
18930-18935, 1998.
3. Van der Bliek, A. M.; Baas, F.; Van der Velde-Koerts, T.; Biedler,
J. L.; Meyers, M. B.; Ozols, R. F.; Hamilton, T. C.; Joenje, H.; Borst,
P.: Genes amplified and overexpressed in human multidrug-resistant
cell lines. Cancer Res. 48: 5927-5932, 1988.
4. Wang, S.-L.; Tam, M.-F.; Ho, Y.-S.; Pai, S.-H.; Kao, M.-C.: Isolation
and molecular cloning of human sorcin a calcium-binding protein in
vincristine-resistant HOB1 lymphoma cells. Biochim. Biophys. Acta 1260:
285-293, 1995.
*FIELD* CN
Rebekah S. Rasooly - updated: 11/10/1998
*FIELD* CD
Victor A. McKusick: 11/28/1988
*FIELD* ED
alopez: 11/10/1998
supermim: 3/16/1992
supermim: 3/20/1990
ddp: 10/27/1989
root: 3/14/1989
root: 3/9/1989
carol: 11/28/1988
*RECORD*
*FIELD* NO
182520
*FIELD* TI
*182520 SORCIN; SRI
;;MULTIDRUG-RESISTANCE COMPLEX, CLASS 4;;
MDR COMPLEX, CLASS 4
read more*FIELD* TX
Van der Bliek et al. (1988) showed that the human homolog of the hamster
sorcin gene resides on chromosome 7 like the P-glycoprotein-encoding
genes (MDR1, 171050; MDR3, 171060). (The sorcin gene was assigned by
Southern analysis of DNA from somatic cell hybrids.) Furthermore, gene
classes designated 4, 5, and 6 were found to be coamplified with MDR1
and MDR3 in a human ovarian carcinoma cell line, which strongly
suggested that the overall structure of the human MDR domain is the same
as that in the hamster and mouse in which the domain which is amplified
to result in multidrug resistance (MDR) comprises not only genes that
encode P-glycoproteins, but also at least 5 unrelated genes, 1 of which
encodes the calcium-binding protein sorcin. Sorcin, coded by the class 4
gene, is a small cytosolic protein that is overproduced in many MDR
cells. It has a sequence similar to the regulatory light-chain of
calpain (114170), including calcium-binding loops of the type found in
calmodulin (114180). Van der Bliek et al. (1988) also found that a
sorcin-related sequence segregated with human chromosome 4. The total
area encompassed by the MDR1, MDR3, and classes 4-6 genes may be as much
as 1500 kb.
Wang et al. (1995) found that the 22-kD sorcin protein was overexpressed
in HOB1/VCR-1.0 cells, a multidrug-resistant human B immunoblastic
lymphoma cell line. By PCR with primers based on the sequence of hamster
sorcin, the authors recovered a partial human sorcin cDNA. Using the
partial cDNA to screen a HOB1/VCR-1.0 library, they isolated cDNAs
corresponding to the entire sorcin coding region. The predicted
198-amino acid human protein shares 95% sequence identity with hamster
sorcin. Human sorcin contains 4 EF-hand calcium-binding motifs. Purified
protein bound calcium in vitro. Southern and Northern blot analysis
revealed that the sorcin gene is amplified and overexpressed in
HOB1/VCR-1.0 cells. No expression was detected in parental HOB1 cells.
Independently, Lee (1996) cloned the human sorcin gene.
In both heart and skeletal muscle, the geometry and close spatial
relationship between sarcolemmal L-type calcium channels and
sarcoplasmic reticulum ryanodine receptors (RyRs, see 180903) is
critical in determining the time course of calcium release and
excitation-contraction coupling. Meyers et al. (1998) stated that sorcin
is 1 of a group of modulators of RyR gating. The authors demonstrated
that sorcin also associates with cardiac and skeletal muscle L-type
calcium channels specifically within the cytoplasmically oriented
C-terminal region of the alpha-1 subunits. See 114204. Meyers et al.
(1998) suggested that sorcin is a candidate regulator of interchannel
communication during excitation-contraction coupling in heart and
skeletal muscle.
*FIELD* RF
1. Lee, W. P.: Purification, cDNA cloning, and expression of human
sorcin in vincristine-resistant HOB1 lymphoma cell lines. Arch. Biochem.
Biophys. 325: 217-226, 1996.
2. Meyers, M. B.; Puri, T. S.; Chien, A. J.; Gao, T.; Hsu, P.-H.;
Hosey, M. M.; Fishman, G. I.: Sorcin associates with the pore-forming
subunit of voltage-dependent L-type Ca(2+) channels. J. Biol. Chem. 273:
18930-18935, 1998.
3. Van der Bliek, A. M.; Baas, F.; Van der Velde-Koerts, T.; Biedler,
J. L.; Meyers, M. B.; Ozols, R. F.; Hamilton, T. C.; Joenje, H.; Borst,
P.: Genes amplified and overexpressed in human multidrug-resistant
cell lines. Cancer Res. 48: 5927-5932, 1988.
4. Wang, S.-L.; Tam, M.-F.; Ho, Y.-S.; Pai, S.-H.; Kao, M.-C.: Isolation
and molecular cloning of human sorcin a calcium-binding protein in
vincristine-resistant HOB1 lymphoma cells. Biochim. Biophys. Acta 1260:
285-293, 1995.
*FIELD* CN
Rebekah S. Rasooly - updated: 11/10/1998
*FIELD* CD
Victor A. McKusick: 11/28/1988
*FIELD* ED
alopez: 11/10/1998
supermim: 3/16/1992
supermim: 3/20/1990
ddp: 10/27/1989
root: 3/14/1989
root: 3/9/1989
carol: 11/28/1988