Full text data of TNFAIP8
TNFAIP8
[Confidence: low (only semi-automatic identification from reviews)]
Tumor necrosis factor alpha-induced protein 8; TNF alpha-induced protein 8 (Head and neck tumor and metastasis-related protein; MDC-3.13; NF-kappa-B-inducible DED-containing protein; NDED; SCC-S2; TNF-induced protein GG2-1)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
Tumor necrosis factor alpha-induced protein 8; TNF alpha-induced protein 8 (Head and neck tumor and metastasis-related protein; MDC-3.13; NF-kappa-B-inducible DED-containing protein; NDED; SCC-S2; TNF-induced protein GG2-1)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
O95379
ID TFIP8_HUMAN Reviewed; 198 AA.
AC O95379; B3KMH1; B3KMI2; Q9P1Q1; Q9UER5; Q9UP47;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-MAY-1999, sequence version 1.
DT 22-JAN-2014, entry version 92.
DE RecName: Full=Tumor necrosis factor alpha-induced protein 8;
DE Short=TNF alpha-induced protein 8;
DE AltName: Full=Head and neck tumor and metastasis-related protein;
DE AltName: Full=MDC-3.13;
DE AltName: Full=NF-kappa-B-inducible DED-containing protein;
DE Short=NDED;
DE AltName: Full=SCC-S2;
DE AltName: Full=TNF-induced protein GG2-1;
GN Name=TNFAIP8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), AND TISSUE SPECIFICITY.
RC TISSUE=Umbilical vein;
RX PubMed=10233894;
RA Horrevoets A.J.G., Fontijn R.D., van Zonneveld A.J., de Vries C.J.M.,
RA ten Cate J.W., Pannekoek H.;
RT "Vascular endothelial genes that are responsive to tumor necrosis
RT factor-alpha in vitro are expressed in atherosclerotic lesions,
RT including inhibitor of apoptosis protein-1, stannin, and two novel
RT genes.";
RL Blood 93:3418-3431(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA Nietfeld W., Meyerhans A.F.;
RT "Identification of cellular factors involved in the differentiation of
RT dendritic cells.";
RL Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 99-198 (ISOFORM 3), IDENTIFICATION,
RP FUNCTION, INDUCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Heart;
RX PubMed=10644768; DOI=10.1074/jbc.275.4.2973;
RA Kumar D., Whiteside T.L., Kasid U.;
RT "Identification of a novel tumor necrosis factor-alpha-inducible gene,
RT SCC-S2, containing the consensus sequence of a death effector domain
RT of fas-associated death domain-like interleukin-1beta-converting
RT enzyme-inhibitory protein.";
RL J. Biol. Chem. 275:2973-2978(2000).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=11346652; DOI=10.1074/jbc.M102464200;
RA You Z., Ouyang H., Lopatin D., Polver P.J., Wang C.-Y.;
RT "Nuclear factor-kappa B-inducible death effector domain-containing
RT protein suppresses tumor necrosis factor-mediated apoptosis by
RT inhibiting caspase-8 activity.";
RL J. Biol. Chem. 276:26398-26404(2001).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=14724590; DOI=10.1038/sj.onc.1207123;
RA Kumar D., Gokhale P., Broustas C., Chakravarty D., Ahmad I., Kasid U.;
RT "Expression of SCC-S2, an antiapoptotic molecule, correlates with
RT enhanced proliferation and tumorigenicity of MDA-MB 435 cells.";
RL Oncogene 23:612-616(2004).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Acts as a negative mediator of apoptosis and may play a
CC role in tumor progression. Suppresses the TNF-mediated apoptosis
CC by inhibiting caspase-8 activity but not the processing of
CC procaspase-8, subsequently resulting in inhibition of BID cleavage
CC and caspase-3 activation.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O95379-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O95379-2; Sequence=VSP_024899;
CC Name=3;
CC IsoId=O95379-3; Sequence=VSP_024898;
CC -!- TISSUE SPECIFICITY: Expressed at high levels in the spleen, lymph
CC node, thymus, thyroid, bone marrow and placenta. Expressed at high
CC levels both in various tumor tissues, unstimulated and cytokine-
CC activated cultured cells. Expressed at low levels in the spinal
CC cord, ovary, lung, adrenal glands, heart, brain, testis and
CC skeletal muscle.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in the fetal liver,
CC lung and kidney.
CC -!- INDUCTION: By nuclear factor-KB (NF-KB) and TNF. Induction by TNF
CC depends upon activation of NF-KB.
CC -!- SIMILARITY: Belongs to the TNFAIP8 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF070671; AAC83229.1; -; mRNA.
DR EMBL; AF099935; AAC72975.1; -; mRNA.
DR EMBL; AF099936; AAC72976.1; -; mRNA.
DR EMBL; CR457137; CAG33418.1; -; mRNA.
DR EMBL; AK001850; BAG50983.1; -; mRNA.
DR EMBL; AK001931; BAG50994.1; -; mRNA.
DR EMBL; CH471086; EAW48914.1; -; Genomic_DNA.
DR EMBL; BC005352; AAH05352.1; -; mRNA.
DR EMBL; BC007014; AAH07014.1; -; mRNA.
DR EMBL; AF098933; AAF29435.1; -; mRNA.
DR RefSeq; NP_001071122.1; NM_001077654.2.
DR RefSeq; NP_055165.2; NM_014350.3.
DR UniGene; Hs.656274; -.
DR ProteinModelPortal; O95379; -.
DR SMR; O95379; 43-195.
DR IntAct; O95379; 2.
DR MINT; MINT-1473332; -.
DR STRING; 9606.ENSP00000408534; -.
DR PaxDb; O95379; -.
DR PRIDE; O95379; -.
DR DNASU; 25816; -.
DR Ensembl; ENST00000274456; ENSP00000274456; ENSG00000145779.
DR Ensembl; ENST00000503646; ENSP00000421848; ENSG00000145779.
DR Ensembl; ENST00000504771; ENSP00000422245; ENSG00000145779.
DR GeneID; 25816; -.
DR KEGG; hsa:25816; -.
DR UCSC; uc003ksi.3; human.
DR CTD; 25816; -.
DR GeneCards; GC05P118604; -.
DR H-InvDB; HIX0005115; -.
DR HGNC; HGNC:17260; TNFAIP8.
DR MIM; 612111; gene.
DR neXtProt; NX_O95379; -.
DR PharmGKB; PA134957136; -.
DR eggNOG; NOG81900; -.
DR HOVERGEN; HBG053286; -.
DR InParanoid; O95379; -.
DR OrthoDB; EOG7P5T2H; -.
DR GeneWiki; TNFAIP8; -.
DR GenomeRNAi; 25816; -.
DR NextBio; 47047; -.
DR PRO; PR:O95379; -.
DR ArrayExpress; O95379; -.
DR Bgee; O95379; -.
DR CleanEx; HS_TNFAIP8; -.
DR Genevestigator; O95379; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; IMP:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; TAS:ProtInc.
DR InterPro; IPR008477; DUF758.
DR PANTHER; PTHR12757; PTHR12757; 1.
DR Pfam; PF05527; DUF758; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Coiled coil; Complete proteome;
KW Cytoplasm; Polymorphism; Reference proteome.
FT CHAIN 1 198 Tumor necrosis factor alpha-induced
FT protein 8.
FT /FTId=PRO_0000285718.
FT COILED 49 83 Potential.
FT VAR_SEQ 1 11 MHSEAEESKEV -> M (in isoform 3).
FT /FTId=VSP_024898.
FT VAR_SEQ 1 10 MHSEAEESKE -> MA (in isoform 2).
FT /FTId=VSP_024899.
FT VARIANT 151 151 S -> C (in dbSNP:rs3203922).
FT /FTId=VAR_032047.
SQ SEQUENCE 198 AA; 23003 MW; D9B8F6FCF9AFC2C3 CRC64;
MHSEAEESKE VATDVFNSKN LAVQAQKKIL GKMVSKSIAT TLIDDTSSEV LDELYRVTRE
YTQNKKEAEK IIKNLIKTVI KLAILYRNNQ FNQDELALME KFKKKVHQLA MTVVSFHQVD
YTFDRNVLSR LLNECREMLH QIIQRHLTAK SHGRVNNVFD HFSDCEFLAA LYNPFGNFKP
HLQKLCDGIN KMLDEENI
//
ID TFIP8_HUMAN Reviewed; 198 AA.
AC O95379; B3KMH1; B3KMI2; Q9P1Q1; Q9UER5; Q9UP47;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
read moreDT 01-MAY-1999, sequence version 1.
DT 22-JAN-2014, entry version 92.
DE RecName: Full=Tumor necrosis factor alpha-induced protein 8;
DE Short=TNF alpha-induced protein 8;
DE AltName: Full=Head and neck tumor and metastasis-related protein;
DE AltName: Full=MDC-3.13;
DE AltName: Full=NF-kappa-B-inducible DED-containing protein;
DE Short=NDED;
DE AltName: Full=SCC-S2;
DE AltName: Full=TNF-induced protein GG2-1;
GN Name=TNFAIP8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), AND TISSUE SPECIFICITY.
RC TISSUE=Umbilical vein;
RX PubMed=10233894;
RA Horrevoets A.J.G., Fontijn R.D., van Zonneveld A.J., de Vries C.J.M.,
RA ten Cate J.W., Pannekoek H.;
RT "Vascular endothelial genes that are responsive to tumor necrosis
RT factor-alpha in vitro are expressed in atherosclerotic lesions,
RT including inhibitor of apoptosis protein-1, stannin, and two novel
RT genes.";
RL Blood 93:3418-3431(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA Nietfeld W., Meyerhans A.F.;
RT "Identification of cellular factors involved in the differentiation of
RT dendritic cells.";
RL Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 99-198 (ISOFORM 3), IDENTIFICATION,
RP FUNCTION, INDUCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Heart;
RX PubMed=10644768; DOI=10.1074/jbc.275.4.2973;
RA Kumar D., Whiteside T.L., Kasid U.;
RT "Identification of a novel tumor necrosis factor-alpha-inducible gene,
RT SCC-S2, containing the consensus sequence of a death effector domain
RT of fas-associated death domain-like interleukin-1beta-converting
RT enzyme-inhibitory protein.";
RL J. Biol. Chem. 275:2973-2978(2000).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=11346652; DOI=10.1074/jbc.M102464200;
RA You Z., Ouyang H., Lopatin D., Polver P.J., Wang C.-Y.;
RT "Nuclear factor-kappa B-inducible death effector domain-containing
RT protein suppresses tumor necrosis factor-mediated apoptosis by
RT inhibiting caspase-8 activity.";
RL J. Biol. Chem. 276:26398-26404(2001).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=14724590; DOI=10.1038/sj.onc.1207123;
RA Kumar D., Gokhale P., Broustas C., Chakravarty D., Ahmad I., Kasid U.;
RT "Expression of SCC-S2, an antiapoptotic molecule, correlates with
RT enhanced proliferation and tumorigenicity of MDA-MB 435 cells.";
RL Oncogene 23:612-616(2004).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Acts as a negative mediator of apoptosis and may play a
CC role in tumor progression. Suppresses the TNF-mediated apoptosis
CC by inhibiting caspase-8 activity but not the processing of
CC procaspase-8, subsequently resulting in inhibition of BID cleavage
CC and caspase-3 activation.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O95379-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O95379-2; Sequence=VSP_024899;
CC Name=3;
CC IsoId=O95379-3; Sequence=VSP_024898;
CC -!- TISSUE SPECIFICITY: Expressed at high levels in the spleen, lymph
CC node, thymus, thyroid, bone marrow and placenta. Expressed at high
CC levels both in various tumor tissues, unstimulated and cytokine-
CC activated cultured cells. Expressed at low levels in the spinal
CC cord, ovary, lung, adrenal glands, heart, brain, testis and
CC skeletal muscle.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in the fetal liver,
CC lung and kidney.
CC -!- INDUCTION: By nuclear factor-KB (NF-KB) and TNF. Induction by TNF
CC depends upon activation of NF-KB.
CC -!- SIMILARITY: Belongs to the TNFAIP8 family.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF070671; AAC83229.1; -; mRNA.
DR EMBL; AF099935; AAC72975.1; -; mRNA.
DR EMBL; AF099936; AAC72976.1; -; mRNA.
DR EMBL; CR457137; CAG33418.1; -; mRNA.
DR EMBL; AK001850; BAG50983.1; -; mRNA.
DR EMBL; AK001931; BAG50994.1; -; mRNA.
DR EMBL; CH471086; EAW48914.1; -; Genomic_DNA.
DR EMBL; BC005352; AAH05352.1; -; mRNA.
DR EMBL; BC007014; AAH07014.1; -; mRNA.
DR EMBL; AF098933; AAF29435.1; -; mRNA.
DR RefSeq; NP_001071122.1; NM_001077654.2.
DR RefSeq; NP_055165.2; NM_014350.3.
DR UniGene; Hs.656274; -.
DR ProteinModelPortal; O95379; -.
DR SMR; O95379; 43-195.
DR IntAct; O95379; 2.
DR MINT; MINT-1473332; -.
DR STRING; 9606.ENSP00000408534; -.
DR PaxDb; O95379; -.
DR PRIDE; O95379; -.
DR DNASU; 25816; -.
DR Ensembl; ENST00000274456; ENSP00000274456; ENSG00000145779.
DR Ensembl; ENST00000503646; ENSP00000421848; ENSG00000145779.
DR Ensembl; ENST00000504771; ENSP00000422245; ENSG00000145779.
DR GeneID; 25816; -.
DR KEGG; hsa:25816; -.
DR UCSC; uc003ksi.3; human.
DR CTD; 25816; -.
DR GeneCards; GC05P118604; -.
DR H-InvDB; HIX0005115; -.
DR HGNC; HGNC:17260; TNFAIP8.
DR MIM; 612111; gene.
DR neXtProt; NX_O95379; -.
DR PharmGKB; PA134957136; -.
DR eggNOG; NOG81900; -.
DR HOVERGEN; HBG053286; -.
DR InParanoid; O95379; -.
DR OrthoDB; EOG7P5T2H; -.
DR GeneWiki; TNFAIP8; -.
DR GenomeRNAi; 25816; -.
DR NextBio; 47047; -.
DR PRO; PR:O95379; -.
DR ArrayExpress; O95379; -.
DR Bgee; O95379; -.
DR CleanEx; HS_TNFAIP8; -.
DR Genevestigator; O95379; -.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; IMP:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; TAS:ProtInc.
DR InterPro; IPR008477; DUF758.
DR PANTHER; PTHR12757; PTHR12757; 1.
DR Pfam; PF05527; DUF758; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Apoptosis; Coiled coil; Complete proteome;
KW Cytoplasm; Polymorphism; Reference proteome.
FT CHAIN 1 198 Tumor necrosis factor alpha-induced
FT protein 8.
FT /FTId=PRO_0000285718.
FT COILED 49 83 Potential.
FT VAR_SEQ 1 11 MHSEAEESKEV -> M (in isoform 3).
FT /FTId=VSP_024898.
FT VAR_SEQ 1 10 MHSEAEESKE -> MA (in isoform 2).
FT /FTId=VSP_024899.
FT VARIANT 151 151 S -> C (in dbSNP:rs3203922).
FT /FTId=VAR_032047.
SQ SEQUENCE 198 AA; 23003 MW; D9B8F6FCF9AFC2C3 CRC64;
MHSEAEESKE VATDVFNSKN LAVQAQKKIL GKMVSKSIAT TLIDDTSSEV LDELYRVTRE
YTQNKKEAEK IIKNLIKTVI KLAILYRNNQ FNQDELALME KFKKKVHQLA MTVVSFHQVD
YTFDRNVLSR LLNECREMLH QIIQRHLTAK SHGRVNNVFD HFSDCEFLAA LYNPFGNFKP
HLQKLCDGIN KMLDEENI
//
MIM
612111
*RECORD*
*FIELD* NO
612111
*FIELD* TI
*612111 TUMOR NECROSIS FACTOR-ALPHA-INDUCED PROTEIN 8; TNFAIP8
;;SCC-S2
*FIELD* TX
read more
CLONING
Using differential display to identify cytokine-responsive genes in
cultured human umbilical vein endothelial cells, Horrevoets et al.
(1999) cloned TNFAIP8, which they designated GG2-1. The deduced protein
contains 188 amino acids. In situ hybridization showed weak expression
in adult human arteries.
Using a partial cDNA that was upregulated in metastatic head and neck
squamous cell carcinomas to screen a heart cDNA library, Kumar et al.
(2000) cloned full-length TNFAIP8, which they designated SCC-S2. The
deduced 188-amino acid protein has a calculated molecular mass of 21 kD.
TNFAIP8 contains a death effector domain (DED) that shares significant
similarity with DED II of the FLIP (see CFLAR, 603599) family of cell
death regulatory proteins. Northern blot analysis detected a 2.0-kb
transcript in most adult and fetal human tissues, with high expression
in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta,
and in fetal liver, lung, and kidney. TNFAIP8 was also expressed in all
cancer cell lines tested.
GENE FUNCTION
Horrevoets et al. (1999) showed that cytokines induced the expression of
TNFAIP8 in human umbilical vein endothelial cells. TNFAIP8 was an early
response gene, with expression peaking between 1.5 and 3 hours.
TNF-alpha (191160), a proapoptotic protein, also induced TNFAIP8
expression. Kumar et al. (2000) showed that TNF-alpha increased TNFAIP8
expression in several human cell lines. Transfection of TNFAIP8 reduced
the number of apoptotic HeLa cells in the presence or absence of
TNF-alpha.
Zhang et al. (2006) found that transfection of TNFAIP8 into a breast
cancer cell line increased its invasiveness in vitro and increased the
frequency of pulmonary colonization of tumor cells in athymic mice.
Treatment of athymic mice with antisense TNFAIP8 inhibited expression
and led to decreased pulmonary metastasis. Antisense inhibition of
endogenous TNFAIP8 correlated with decreased expression of VEGFR2 (KDR;
191306) in tumor cells and human lung microvascular endothelial cells
and loss of endothelial cell viability. Downregulation of TNPAIP8 in
tumor cells was associated with decreased expression of
metastasis-related proteins MMP1 (120353) and MMP9 (120361).
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the TNFAIP8
gene to chromosome 5 (TMAP RH16153).
*FIELD* RF
1. Horrevoets, A. J. G.; Fontijn, R. D.; van Zonneveld, A. J.; de
Vries, C. J. M.; ten Cate, J. W.; Pannekoek, H.: Vascular endothelial
genes that are responsive to tumor necrosis factor-alpha in vitro
are expressed in atherosclerotic lesions, including inhibitor of apoptosis
protein-1, stannin, and two novel genes. Blood 93: 3418-3431, 1999.
2. Kumar, D.; Whiteside, T. L.; Kasid, U.: Identification of a novel
tumor necrosis factor-alpha-inducible gene, SCC-S2, containing the
consensus sequence of a death effector domain of Fas-associated death
domain-like interleukin-1-beta-converting enzyme-inhibitory protein. J.
Biol. Chem. 275: 2973-2978, 2000.
3. Zhang, C.; Chakravarty, D.; Sakabe, I.; Mewani, R. R.; Boudreau,
H. E.; Kumar, D.; Ahmad, I.; Kasid, U. N.: Role of SCC-S2 in experimental
metastasis and modulation of VEGFR-2, MMP-1, and MMP-9 expression. Molec.
Ther. 13: 947-955, 2006.
*FIELD* CD
Patricia A. Hartz: 6/16/2008
*FIELD* ED
wwang: 06/17/2008
*RECORD*
*FIELD* NO
612111
*FIELD* TI
*612111 TUMOR NECROSIS FACTOR-ALPHA-INDUCED PROTEIN 8; TNFAIP8
;;SCC-S2
*FIELD* TX
read more
CLONING
Using differential display to identify cytokine-responsive genes in
cultured human umbilical vein endothelial cells, Horrevoets et al.
(1999) cloned TNFAIP8, which they designated GG2-1. The deduced protein
contains 188 amino acids. In situ hybridization showed weak expression
in adult human arteries.
Using a partial cDNA that was upregulated in metastatic head and neck
squamous cell carcinomas to screen a heart cDNA library, Kumar et al.
(2000) cloned full-length TNFAIP8, which they designated SCC-S2. The
deduced 188-amino acid protein has a calculated molecular mass of 21 kD.
TNFAIP8 contains a death effector domain (DED) that shares significant
similarity with DED II of the FLIP (see CFLAR, 603599) family of cell
death regulatory proteins. Northern blot analysis detected a 2.0-kb
transcript in most adult and fetal human tissues, with high expression
in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta,
and in fetal liver, lung, and kidney. TNFAIP8 was also expressed in all
cancer cell lines tested.
GENE FUNCTION
Horrevoets et al. (1999) showed that cytokines induced the expression of
TNFAIP8 in human umbilical vein endothelial cells. TNFAIP8 was an early
response gene, with expression peaking between 1.5 and 3 hours.
TNF-alpha (191160), a proapoptotic protein, also induced TNFAIP8
expression. Kumar et al. (2000) showed that TNF-alpha increased TNFAIP8
expression in several human cell lines. Transfection of TNFAIP8 reduced
the number of apoptotic HeLa cells in the presence or absence of
TNF-alpha.
Zhang et al. (2006) found that transfection of TNFAIP8 into a breast
cancer cell line increased its invasiveness in vitro and increased the
frequency of pulmonary colonization of tumor cells in athymic mice.
Treatment of athymic mice with antisense TNFAIP8 inhibited expression
and led to decreased pulmonary metastasis. Antisense inhibition of
endogenous TNFAIP8 correlated with decreased expression of VEGFR2 (KDR;
191306) in tumor cells and human lung microvascular endothelial cells
and loss of endothelial cell viability. Downregulation of TNPAIP8 in
tumor cells was associated with decreased expression of
metastasis-related proteins MMP1 (120353) and MMP9 (120361).
MAPPING
The International Radiation Hybrid Mapping Consortium mapped the TNFAIP8
gene to chromosome 5 (TMAP RH16153).
*FIELD* RF
1. Horrevoets, A. J. G.; Fontijn, R. D.; van Zonneveld, A. J.; de
Vries, C. J. M.; ten Cate, J. W.; Pannekoek, H.: Vascular endothelial
genes that are responsive to tumor necrosis factor-alpha in vitro
are expressed in atherosclerotic lesions, including inhibitor of apoptosis
protein-1, stannin, and two novel genes. Blood 93: 3418-3431, 1999.
2. Kumar, D.; Whiteside, T. L.; Kasid, U.: Identification of a novel
tumor necrosis factor-alpha-inducible gene, SCC-S2, containing the
consensus sequence of a death effector domain of Fas-associated death
domain-like interleukin-1-beta-converting enzyme-inhibitory protein. J.
Biol. Chem. 275: 2973-2978, 2000.
3. Zhang, C.; Chakravarty, D.; Sakabe, I.; Mewani, R. R.; Boudreau,
H. E.; Kumar, D.; Ahmad, I.; Kasid, U. N.: Role of SCC-S2 in experimental
metastasis and modulation of VEGFR-2, MMP-1, and MMP-9 expression. Molec.
Ther. 13: 947-955, 2006.
*FIELD* CD
Patricia A. Hartz: 6/16/2008
*FIELD* ED
wwang: 06/17/2008