Full text data of TIAF1
TIAF1
[Confidence: low (only semi-automatic identification from reviews)]
TGFB1-induced anti-apoptotic factor 1 (12 kDa TGF-beta-1-induced antiapoptotic factor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
TGFB1-induced anti-apoptotic factor 1 (12 kDa TGF-beta-1-induced antiapoptotic factor)
Note: presumably soluble (membrane word is not in UniProt keywords or features)
UniProt
O95411
ID TIAF1_HUMAN Reviewed; 115 AA.
AC O95411; A2RRE2; Q6PEG2;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
read moreDT 31-AUG-2004, sequence version 2.
DT 22-JAN-2014, entry version 96.
DE RecName: Full=TGFB1-induced anti-apoptotic factor 1;
DE AltName: Full=12 kDa TGF-beta-1-induced antiapoptotic factor;
GN Name=TIAF1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC TISSUE=Mammary gland;
RX PubMed=9918798; DOI=10.1006/bbrc.1998.9846;
RA Chang N.-S., Mattison J., Cao H., Pratt N., Zhao Y., Lee C.;
RT "Cloning and characterization of a novel transforming growth factor-
RT beta1-induced TIAF1 protein that inhibits tumor necrosis factor
RT cytotoxicity.";
RL Biochem. Biophys. Res. Commun. 253:743-749(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R.,
RA Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N.,
RA Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B.,
RA Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J.,
RA Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E.,
RA Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J.,
RA Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C.,
RA Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in
RT the human lineage.";
RL Nature 440:1045-1049(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12829915; DOI=10.1097/01.TP.0000069829.71088.88;
RA van der Leij J., van den Berg A., Albrecht E.W., Blokzijl T.,
RA Roozendaal R., Gouw A.S., de Jong K.P., Stegeman C.A., van Goor H.,
RA Chang N.S., Poppema S.;
RT "High expression of TIAF-1 in chronic kidney and liver allograft
RT rejection and in activated T-helper cells.";
RL Transplantation 75:2076-2082(2003).
CC -!- FUNCTION: Inhibits the cytotoxic effects of TNF-alpha and
CC overexpressed TNF receptor adapters TRADD, FADD, and RIPK1.
CC Involved in TGF-beta1 inhibition of IkappaB-alpha expression and
CC suppression of TNF-mediated IkappaB-alpha degradation.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Not detectable in normal kidney and liver. Up-
CC regulated in chronic and acute allograft rejection: expressed in
CC the inflammatory infiltrate and in tubular epithelial cells.
CC -!- CAUTION: The TIAF1 protein is coded in the 3'-UTR region of
CC MYO18A. Does not seem to exist in orthologs.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF105277; AAD04172.1; -; mRNA.
DR EMBL; AC024619; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471159; EAW51180.1; -; Genomic_DNA.
DR EMBL; BC047669; AAH47669.1; -; mRNA.
DR EMBL; BC058075; AAH58075.1; -; mRNA.
DR EMBL; BC131561; AAI31562.1; -; mRNA.
DR RefSeq; NP_004731.2; NM_004740.3.
DR UniGene; Hs.462590; -.
DR UniGene; Hs.743384; -.
DR ProteinModelPortal; O95411; -.
DR IntAct; O95411; 1.
DR MINT; MINT-1134378; -.
DR STRING; 9606.ENSP00000386130; -.
DR PaxDb; O95411; -.
DR PRIDE; O95411; -.
DR Ensembl; ENST00000359450; ENSP00000352424; ENSG00000221995.
DR Ensembl; ENST00000408971; ENSP00000386130; ENSG00000221995.
DR GeneID; 9220; -.
DR KEGG; hsa:9220; -.
DR UCSC; uc002hdv.1; human.
DR CTD; 9220; -.
DR GeneCards; GC17M027401; -.
DR HGNC; HGNC:11803; TIAF1.
DR HPA; HPA051129; -.
DR MIM; 609517; gene.
DR neXtProt; NX_O95411; -.
DR PharmGKB; PA36512; -.
DR HOVERGEN; HBG079257; -.
DR OMA; WVEQAYA; -.
DR OrthoDB; EOG73V6NG; -.
DR GeneWiki; TIAF1; -.
DR GenomeRNAi; 9220; -.
DR NextBio; 34567; -.
DR PRO; PR:O95411; -.
DR Bgee; O95411; -.
DR CleanEx; HS_TIAF1; -.
DR Genevestigator; O95411; -.
DR GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0007249; P:I-kappaB kinase/NF-kappaB cascade; IDA:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
PE 2: Evidence at transcript level;
KW Apoptosis; Complete proteome; Nucleus; Reference proteome.
FT CHAIN 1 115 TGFB1-induced anti-apoptotic factor 1.
FT /FTId=PRO_0000072534.
FT CONFLICT 49 49 A -> V (in Ref. 1; AAD04172).
SQ SEQUENCE 115 AA; 12414 MW; C71C03671CBDB3B9 CRC64;
MSSPSSPFRE QSFLCAAGDA GEESRVQVLK NEVRRGSPVL LGWVEQAYAD KCVCGPSAPP
APTPPSLSQR VMCNDLFKVN PFQLQQFRAD PSTASLLLCP GGLDHKLNLR GKAWG
//
ID TIAF1_HUMAN Reviewed; 115 AA.
AC O95411; A2RRE2; Q6PEG2;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
read moreDT 31-AUG-2004, sequence version 2.
DT 22-JAN-2014, entry version 96.
DE RecName: Full=TGFB1-induced anti-apoptotic factor 1;
DE AltName: Full=12 kDa TGF-beta-1-induced antiapoptotic factor;
GN Name=TIAF1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC TISSUE=Mammary gland;
RX PubMed=9918798; DOI=10.1006/bbrc.1998.9846;
RA Chang N.-S., Mattison J., Cao H., Pratt N., Zhao Y., Lee C.;
RT "Cloning and characterization of a novel transforming growth factor-
RT beta1-induced TIAF1 protein that inhibits tumor necrosis factor
RT cytotoxicity.";
RL Biochem. Biophys. Res. Commun. 253:743-749(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R.,
RA Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N.,
RA Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B.,
RA Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J.,
RA Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E.,
RA Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J.,
RA Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C.,
RA Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in
RT the human lineage.";
RL Nature 440:1045-1049(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skin, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12829915; DOI=10.1097/01.TP.0000069829.71088.88;
RA van der Leij J., van den Berg A., Albrecht E.W., Blokzijl T.,
RA Roozendaal R., Gouw A.S., de Jong K.P., Stegeman C.A., van Goor H.,
RA Chang N.S., Poppema S.;
RT "High expression of TIAF-1 in chronic kidney and liver allograft
RT rejection and in activated T-helper cells.";
RL Transplantation 75:2076-2082(2003).
CC -!- FUNCTION: Inhibits the cytotoxic effects of TNF-alpha and
CC overexpressed TNF receptor adapters TRADD, FADD, and RIPK1.
CC Involved in TGF-beta1 inhibition of IkappaB-alpha expression and
CC suppression of TNF-mediated IkappaB-alpha degradation.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Not detectable in normal kidney and liver. Up-
CC regulated in chronic and acute allograft rejection: expressed in
CC the inflammatory infiltrate and in tubular epithelial cells.
CC -!- CAUTION: The TIAF1 protein is coded in the 3'-UTR region of
CC MYO18A. Does not seem to exist in orthologs.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; AF105277; AAD04172.1; -; mRNA.
DR EMBL; AC024619; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471159; EAW51180.1; -; Genomic_DNA.
DR EMBL; BC047669; AAH47669.1; -; mRNA.
DR EMBL; BC058075; AAH58075.1; -; mRNA.
DR EMBL; BC131561; AAI31562.1; -; mRNA.
DR RefSeq; NP_004731.2; NM_004740.3.
DR UniGene; Hs.462590; -.
DR UniGene; Hs.743384; -.
DR ProteinModelPortal; O95411; -.
DR IntAct; O95411; 1.
DR MINT; MINT-1134378; -.
DR STRING; 9606.ENSP00000386130; -.
DR PaxDb; O95411; -.
DR PRIDE; O95411; -.
DR Ensembl; ENST00000359450; ENSP00000352424; ENSG00000221995.
DR Ensembl; ENST00000408971; ENSP00000386130; ENSG00000221995.
DR GeneID; 9220; -.
DR KEGG; hsa:9220; -.
DR UCSC; uc002hdv.1; human.
DR CTD; 9220; -.
DR GeneCards; GC17M027401; -.
DR HGNC; HGNC:11803; TIAF1.
DR HPA; HPA051129; -.
DR MIM; 609517; gene.
DR neXtProt; NX_O95411; -.
DR PharmGKB; PA36512; -.
DR HOVERGEN; HBG079257; -.
DR OMA; WVEQAYA; -.
DR OrthoDB; EOG73V6NG; -.
DR GeneWiki; TIAF1; -.
DR GenomeRNAi; 9220; -.
DR NextBio; 34567; -.
DR PRO; PR:O95411; -.
DR Bgee; O95411; -.
DR CleanEx; HS_TIAF1; -.
DR Genevestigator; O95411; -.
DR GO; GO:0005634; C:nucleus; NAS:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0007249; P:I-kappaB kinase/NF-kappaB cascade; IDA:UniProtKB.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
PE 2: Evidence at transcript level;
KW Apoptosis; Complete proteome; Nucleus; Reference proteome.
FT CHAIN 1 115 TGFB1-induced anti-apoptotic factor 1.
FT /FTId=PRO_0000072534.
FT CONFLICT 49 49 A -> V (in Ref. 1; AAD04172).
SQ SEQUENCE 115 AA; 12414 MW; C71C03671CBDB3B9 CRC64;
MSSPSSPFRE QSFLCAAGDA GEESRVQVLK NEVRRGSPVL LGWVEQAYAD KCVCGPSAPP
APTPPSLSQR VMCNDLFKVN PFQLQQFRAD PSTASLLLCP GGLDHKLNLR GKAWG
//
MIM
609517
*RECORD*
*FIELD* NO
609517
*FIELD* TI
*609517 TGFB1-INDUCED ANTIAPOPTOTIC FACTOR 1; TIAF1
;;MYOSIN CONTAINING PDZ DOMAIN; MYSPDZ;;
read moreMOLECULE ASSOCIATED WITH JAK3 N TERMINUS; MAJN;;
KIAA0216
*FIELD* TX
CLONING
By sequencing clones obtained from a size-fractionated immature myeloid
cell line cDNA library, Nagase et al. (1996) cloned TIAF1, which they
designated KIAA0216. The deduced 1,581-amino acid protein shares
significant similarity with rat myosin heavy chain, and it contains an
ATP/GTP-binding site motif A. Northern blot analysis detected TIAF1 in
all tissues and cell lines examined, with highest expression in skeletal
muscle.
Using differential display to identify genes whose expression correlated
with the ability of mouse stromal cells to support hematopoiesis,
Furusawa et al. (2000) cloned Tiaf1, which they designated Myspdz. The
deduced 2,035-amino acid protein shares 94% homology with KIAA0216, but
it contains an additional 458 N-terminal amino acids. By database
analysis, Furusawa et al. (2000) identified a human genomic clone
containing an exon with significant similarity to nucleotides 1 to 946
of the mouse Tiaf1 gene, suggesting that human TIAF1 may also encode a
longer protein. Mouse Tiaf1 contains an N-terminal KE-rich region,
followed by a PDZ domain, a head region with an ATP-binding site, a neck
region with an IQ motif, and a coiled-coil C-terminal domain. Northern
blot analysis detected Tiaf1 in all tissues examined. A 10.5-kb
transcript was detected in heart and skeletal muscle, and a 7.0-kb
transcript was detected in hematopoietic tissues and cells. Western blot
analysis detected Tiaf1 at an apparent molecular mass of 230 kD.
Immunostaining localized Tiaf1 in a filamentous network slightly
concentrated in the perinuclear region of mouse stromal cells.
GENE FUNCTION
Ji et al. (2000) found that the C-terminal myosin tail motif of TIAF1
interacts with the N terminus of JAK3 (600173).
Schultz et al. (2004) showed that TIAF1 and p53 (TP53; 191170) induced
apoptosis in human U937 myocytoma cells in both synergistic and
antagonistic manners. At optimal levels, both TIAF1 and p53 mediated
apoptosis cooperatively. Both proteins also suppressed
adherence-independent growth in a mouse fibroblast cell line. In
contrast, initiation of apoptosis by overexpressed TIAF1 was blocked by
low doses of p53, and vice versa. Ectopic p53 blocked apoptosis in U937
cells stably expressing TIAF1. TIAF1 and p53 did not appear to
physically interact; however, nuclear translocation of phosphorylated
p53 was significantly reduced in TIAF1-silenced cells. Schultz et al.
(2004) concluded that TIAF1 likely participates in the nuclear
translocation of activated p53.
GENE STRUCTURE
The TIAF1 gene resides within the 3-prime untranslated region of the
MYO18A gene (610067) (Scott, 2006).
MAPPING
By radiation and somatic cell hybrid analyses, Nagase et al. (1996)
mapped the TIAF1 gene to chromosome 17.
*FIELD* RF
1. Furusawa, T.; Ikawa, S.; Yanai, N.; Obinata, M.: Isolation of
a novel PDZ-containing myosin from hematopoietic supportive bone marrow
stromal cell lines. Biochem. Biophys. Res. Commun. 270: 67-75, 2000.
2. Ji, H.; Zhai, Q.; Zhu, J.; Yan, M.; Sun, L.; Liu, X.; Zheng, Z.
: A novel protein MAJN binds to Jak3 and inhibits apoptosis induced
by IL-2 deprival. Biochem. Biophys. Res. Commun. 270: 267-271, 2000.
3. Nagase, T.; Seki, N.; Ishikawa, K.; Ohira, M.; Kawarabayasi, Y.;
Ohara, O.; Tanaka, A.; Kotani, H.; Miyajima, N.; Nomura, N.: Prediction
of the coding sequences of unidentified human genes. VI. The coding
sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis
of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329,
1996.
4. Schultz, L.; Khera, S.; Sleve, D.; Heath, J.; Chang, N.-S.: TIAF1
and p53 functionally interact in mediating apoptosis and silencing
of TIAF1 abolishes nuclear translocation of serine 15-phosphorylated
p53. DNA Cell Biol. 23: 67-74, 2004.
5. Scott, A. F.: Personal Communication. Baltimore, Md. 4/20/2006.
*FIELD* CD
Patricia A. Hartz: 8/5/2005
*FIELD* ED
alopez: 04/20/2006
alopez: 4/7/2006
mgross: 8/5/2005
*RECORD*
*FIELD* NO
609517
*FIELD* TI
*609517 TGFB1-INDUCED ANTIAPOPTOTIC FACTOR 1; TIAF1
;;MYOSIN CONTAINING PDZ DOMAIN; MYSPDZ;;
read moreMOLECULE ASSOCIATED WITH JAK3 N TERMINUS; MAJN;;
KIAA0216
*FIELD* TX
CLONING
By sequencing clones obtained from a size-fractionated immature myeloid
cell line cDNA library, Nagase et al. (1996) cloned TIAF1, which they
designated KIAA0216. The deduced 1,581-amino acid protein shares
significant similarity with rat myosin heavy chain, and it contains an
ATP/GTP-binding site motif A. Northern blot analysis detected TIAF1 in
all tissues and cell lines examined, with highest expression in skeletal
muscle.
Using differential display to identify genes whose expression correlated
with the ability of mouse stromal cells to support hematopoiesis,
Furusawa et al. (2000) cloned Tiaf1, which they designated Myspdz. The
deduced 2,035-amino acid protein shares 94% homology with KIAA0216, but
it contains an additional 458 N-terminal amino acids. By database
analysis, Furusawa et al. (2000) identified a human genomic clone
containing an exon with significant similarity to nucleotides 1 to 946
of the mouse Tiaf1 gene, suggesting that human TIAF1 may also encode a
longer protein. Mouse Tiaf1 contains an N-terminal KE-rich region,
followed by a PDZ domain, a head region with an ATP-binding site, a neck
region with an IQ motif, and a coiled-coil C-terminal domain. Northern
blot analysis detected Tiaf1 in all tissues examined. A 10.5-kb
transcript was detected in heart and skeletal muscle, and a 7.0-kb
transcript was detected in hematopoietic tissues and cells. Western blot
analysis detected Tiaf1 at an apparent molecular mass of 230 kD.
Immunostaining localized Tiaf1 in a filamentous network slightly
concentrated in the perinuclear region of mouse stromal cells.
GENE FUNCTION
Ji et al. (2000) found that the C-terminal myosin tail motif of TIAF1
interacts with the N terminus of JAK3 (600173).
Schultz et al. (2004) showed that TIAF1 and p53 (TP53; 191170) induced
apoptosis in human U937 myocytoma cells in both synergistic and
antagonistic manners. At optimal levels, both TIAF1 and p53 mediated
apoptosis cooperatively. Both proteins also suppressed
adherence-independent growth in a mouse fibroblast cell line. In
contrast, initiation of apoptosis by overexpressed TIAF1 was blocked by
low doses of p53, and vice versa. Ectopic p53 blocked apoptosis in U937
cells stably expressing TIAF1. TIAF1 and p53 did not appear to
physically interact; however, nuclear translocation of phosphorylated
p53 was significantly reduced in TIAF1-silenced cells. Schultz et al.
(2004) concluded that TIAF1 likely participates in the nuclear
translocation of activated p53.
GENE STRUCTURE
The TIAF1 gene resides within the 3-prime untranslated region of the
MYO18A gene (610067) (Scott, 2006).
MAPPING
By radiation and somatic cell hybrid analyses, Nagase et al. (1996)
mapped the TIAF1 gene to chromosome 17.
*FIELD* RF
1. Furusawa, T.; Ikawa, S.; Yanai, N.; Obinata, M.: Isolation of
a novel PDZ-containing myosin from hematopoietic supportive bone marrow
stromal cell lines. Biochem. Biophys. Res. Commun. 270: 67-75, 2000.
2. Ji, H.; Zhai, Q.; Zhu, J.; Yan, M.; Sun, L.; Liu, X.; Zheng, Z.
: A novel protein MAJN binds to Jak3 and inhibits apoptosis induced
by IL-2 deprival. Biochem. Biophys. Res. Commun. 270: 267-271, 2000.
3. Nagase, T.; Seki, N.; Ishikawa, K.; Ohira, M.; Kawarabayasi, Y.;
Ohara, O.; Tanaka, A.; Kotani, H.; Miyajima, N.; Nomura, N.: Prediction
of the coding sequences of unidentified human genes. VI. The coding
sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis
of cDNA clones from cell line KG-1 and brain. DNA Res. 3: 321-329,
1996.
4. Schultz, L.; Khera, S.; Sleve, D.; Heath, J.; Chang, N.-S.: TIAF1
and p53 functionally interact in mediating apoptosis and silencing
of TIAF1 abolishes nuclear translocation of serine 15-phosphorylated
p53. DNA Cell Biol. 23: 67-74, 2004.
5. Scott, A. F.: Personal Communication. Baltimore, Md. 4/20/2006.
*FIELD* CD
Patricia A. Hartz: 8/5/2005
*FIELD* ED
alopez: 04/20/2006
alopez: 4/7/2006
mgross: 8/5/2005