RBCC

Full text data of ZW10

ZW10 [Confidence: low (only semi-automatic identification from reviews)]
Centromere/kinetochore protein zw10 homolog

UniProt O43264
ID ZW10_HUMAN Reviewed; 779 AA.
AC O43264;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
read moreDT 23-JAN-2007, sequence version 3.
DT 22-JAN-2014, entry version 116.
DE RecName: Full=Centromere/kinetochore protein zw10 homolog;
GN Name=ZW10;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Cervix carcinoma;
RX PubMed=9298984; DOI=10.1083/jcb.138.6.1289;
RA Starr D.A., Williams B.C., Li Z., Etemad-Moghadam B., Dawe R.K.,
RA Goldberg M.L.;
RT "Conservation of the centromere/kinetochore protein ZW10.";
RL J. Cell Biol. 138:1289-1301(1997).
RN [2]
RP PROTEIN SEQUENCE OF 2-14.
RC TISSUE=Platelet;
RX PubMed=12665801; DOI=10.1038/nbt810;
RA Gevaert K., Goethals M., Martens L., Van Damme J., Staes A.,
RA Thomas G.R., Vandekerckhove J.;
RT "Exploring proteomes and analyzing protein processing by mass
RT spectrometric identification of sorted N-terminal peptides.";
RL Nat. Biotechnol. 21:566-569(2003).
RN [3]
RP PROTEIN SEQUENCE OF 2-14 AND 161-170, CLEAVAGE OF INITIATOR
RP METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
RC TISSUE=Hepatoma;
RA Bienvenut W.V., Boldt K., von Kriegsheim A.F., Kolch W.;
RL Submitted (JUL-2007) to UniProtKB.
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11590237;
RA Scaeerou F., Starr D.A., Piano F., Papoulas O., Karess R.E.,
RA Goldberg M.L.;
RT "The ZW10 and Rough Deal checkpoint proteins function together in a
RT large, evolutionarily conserved complex targeted to the kinetochore.";
RL J. Cell Sci. 114:3103-3114(2001).
RN [5]
RP INTERACTION WITH ZWILCH AND ZW10.
RX PubMed=12686595; DOI=10.1091/mbc.E02-09-0624;
RA Williams B.C., Li Z., Liu S., Williams E.V., Leung G., Yen T.J.,
RA Goldberg M.L.;
RT "Zwilch, a new component of the ZW10/ROD complex required for
RT kinetochore functions.";
RL Mol. Biol. Cell 14:1379-1391(2003).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY,
RP IDENTIFICATION IN A COMPLEX WITH STX18; USE1L; SEC22B AND RINT1, AND
RP INTERACTION WITH RINT1.
RX PubMed=15029241; DOI=10.1038/sj.emboj.7600135;
RA Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M.,
RA Tani K., Yamamoto A., Tohyama M., Tagaya M.;
RT "Implication of ZW10 in membrane trafficking between the endoplasmic
RT reticulum and Golgi.";
RL EMBO J. 23:1267-1278(2004).
RN [7]
RP INTERACTION WITH BNIP1 THROUGH RINT1.
RX PubMed=15272311; DOI=10.1038/sj.emboj.7600333;
RA Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K.,
RA Nagahama M., Tani K., Yamamoto A., Tagaya M.;
RT "Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane
RT fusion.";
RL EMBO J. 23:3216-3226(2004).
RN [8]
RP FUNCTION.
RX PubMed=15094189; DOI=10.1016/j.gene.2004.01.028;
RA Musio A., Mariani T., Montagna C., Zambroni D., Ascoli C., Ried T.,
RA Vezzoni P.;
RT "Recapitulation of the Roberts syndrome cellular phenotype by
RT inhibition of INCENP, ZWINT-1 and ZW10 genes.";
RL Gene 331:33-40(2004).
RN [9]
RP FUNCTION, INTERACTION WITH ZWINT, AND SUBCELLULAR LOCATION.
RX PubMed=15485811; DOI=10.1074/jbc.M407588200;
RA Wang H., Hu X., Ding X., Dou Z., Yang Z., Shaw A.W., Teng M.,
RA Cleveland D.W., Goldberg M.L., Niu L., Yao X.;
RT "Human Zwint-1 specifies localization of Zeste White 10 to
RT kinetochores and is essential for mitotic checkpoint signaling.";
RL J. Biol. Chem. 279:54590-54598(2004).
RN [10]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH
RP C19ORF25; KNTC1; RINT1; ZWILCH AND ZWINT, AND SUBCELLULAR LOCATION.
RX PubMed=15824131; DOI=10.1083/jcb.200411118;
RA Kops G.J.P.L., Kim Y., Weaver B.A.A., Mao Y., McLeod I.,
RA Yates J.R. III, Tagaya M., Cleveland D.W.;
RT "ZW10 links mitotic checkpoint signaling to the structural
RT kinetochore.";
RL J. Cell Biol. 169:49-60(2005).
RN [11]
RP INTERACTION WITH ZWINT, AND SUBCELLULAR LOCATION.
RX PubMed=16732327; DOI=10.1038/sj.onc.1209687;
RA Lin Y.-T., Chen Y., Wu G., Lee W.-H.;
RT "Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for
RT faithful chromosome segregation and spindle checkpoint control.";
RL Oncogene 25:6901-6914(2006).
RN [12]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,
RA Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in
RT a refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [13]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-777, AND MASS SPECTROMETRY.
RX PubMed=19608861; DOI=10.1126/science.1175371;
RA Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M.,
RA Walther T.C., Olsen J.V., Mann M.;
RT "Lysine acetylation targets protein complexes and co-regulates major
RT cellular functions.";
RL Science 325:834-840(2009).
RN [14]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Essential component of the mitotic checkpoint, which
CC prevents cells from prematurely exiting mitosis. Required for the
CC assembly of the dynein-dynactin and MAD1-MAD2 complexes onto
CC kinetochores. Involved in regulation of membrane traffic between
CC the Golgi and the endoplasmic reticulum.
CC -!- SUBUNIT: Associated with a SNARE complex consisting of STX18,
CC USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with
CC RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex
CC composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25,
CC KNTC1, and ZWINT.
CC -!- INTERACTION:
CC O95229:ZWINT; NbExp=6; IntAct=EBI-1001217, EBI-1001132;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Endoplasmic reticulum membrane;
CC Peripheral membrane protein. Chromosome, centromere, kinetochore.
CC Cytoplasm, cytoskeleton, spindle. Note=Dynamic pattern of
CC localization during the cell cycle. In most cells at interphase,
CC present diffusely in the cytoplasm. In prometaphase, associated
CC with the kinetochore. At metaphase, detected both at the
CC kinetochores and, most prominently, at the spindle, particularly
CC at the spindle poles. In very early anaphase, detected on
CC segregating kinetochores. In late anaphase and telophase,
CC accumulates at the spindle midzone.
CC -!- TISSUE SPECIFICITY: Widely expressed.
CC -!- DEVELOPMENTAL STAGE: No significant variation in expression during
CC cell cycle.
CC -!- MISCELLANEOUS: Overexpression as well as silencing of ZW10
CC disrupts the morphology of the ER-Golgi intermediate compartment
CC as well as the Golgi apparatus and slows down ER-Golgi transport.
CC -!- SIMILARITY: Belongs to the ZW10 family.
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DR EMBL; U54996; AAB88237.1; -; mRNA.
DR RefSeq; NP_004715.1; NM_004724.3.
DR UniGene; Hs.503886; -.
DR ProteinModelPortal; O43264; -.
DR DIP; DIP-36471N; -.
DR IntAct; O43264; 14.
DR MINT; MINT-2999296; -.
DR STRING; 9606.ENSP00000200135; -.
DR PhosphoSite; O43264; -.
DR PaxDb; O43264; -.
DR PeptideAtlas; O43264; -.
DR PRIDE; O43264; -.
DR Ensembl; ENST00000200135; ENSP00000200135; ENSG00000086827.
DR GeneID; 9183; -.
DR KEGG; hsa:9183; -.
DR UCSC; uc001poe.3; human.
DR CTD; 9183; -.
DR GeneCards; GC11M113603; -.
DR HGNC; HGNC:13194; ZW10.
DR HPA; CAB011565; -.
DR MIM; 603954; gene.
DR neXtProt; NX_O43264; -.
DR PharmGKB; PA37759; -.
DR eggNOG; NOG299329; -.
DR HOGENOM; HOG000007982; -.
DR HOVERGEN; HBG004603; -.
DR InParanoid; O43264; -.
DR KO; K11578; -.
DR OMA; EHPSPSE; -.
DR OrthoDB; EOG718KBR; -.
DR PhylomeDB; O43264; -.
DR Reactome; REACT_115566; Cell Cycle.
DR Reactome; REACT_21300; Mitotic M-M/G1 phases.
DR GeneWiki; ZW10; -.
DR GenomeRNAi; 9183; -.
DR NextBio; 34429; -.
DR PRO; PR:O43264; -.
DR ArrayExpress; O43264; -.
DR Bgee; O43264; -.
DR CleanEx; HS_ZW10; -.
DR Genevestigator; O43264; -.
DR GO; GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:HGNC.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000776; C:kinetochore; IDA:UniProtKB.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:HGNC.
DR GO; GO:0000922; C:spindle pole; IDA:UniProtKB.
DR GO; GO:0019237; F:centromeric DNA binding; TAS:ProtInc.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:HGNC.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IMP:UniProtKB.
DR GO; GO:0007126; P:meiosis; TAS:ProtInc.
DR GO; GO:0007093; P:mitotic cell cycle checkpoint; IDA:UniProtKB.
DR GO; GO:0007080; P:mitotic metaphase plate congression; IMP:UniProtKB.
DR GO; GO:0006461; P:protein complex assembly; IDA:HGNC.
DR GO; GO:0034501; P:protein localization to kinetochore; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0007096; P:regulation of exit from mitosis; IDA:HGNC.
DR InterPro; IPR009361; RZZ-complex_Zw10.
DR Pfam; PF06248; Zw10; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Centromere; Chromosome;
KW Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton;
KW Direct protein sequencing; Endoplasmic reticulum; ER-Golgi transport;
KW Kinetochore; Membrane; Mitosis; Polymorphism; Protein transport;
KW Reference proteome; Transport.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 779 Centromere/kinetochore protein zw10
FT homolog.
FT /FTId=PRO_0000184957.
FT REGION 2 317 Interaction with RINT1.
FT REGION 2 81 Interaction with ZWINT.
FT COILED 14 130 Potential.
FT MOD_RES 2 2 N-acetylalanine.
FT MOD_RES 777 777 N6-acetyllysine.
FT VARIANT 77 77 I -> M (in dbSNP:rs2271796).
FT /FTId=VAR_053537.
SQ SEQUENCE 779 AA; 88829 MW; 9C38186153886481 CRC64;
MASFVTEVLA HSGRLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL PSMQSAQGLI
TQVDKLSEDI DLLKSRIESE VRRDLHVSTG EFTDLKQQLE RDSVVLSLLK QLQEFSTAIE
EYNCALTEKK YVTGAQRLEE AQKCLKLLKS RKCFDLKILK SLSMELTIQK QNILYHLGEE
WQKLIVWKFP PSKDTSSLES YLQTELHLYT EQSHKEEKTP MPPISSVLLA FSVLGELHSK
LKSFGQMLLK YILRPLASCP SLHAVIESQP NIVIIRFESI MTNLEYPSPS EVFTKIRLVL
EVLQKQLLDL PLDTDLENEK TSTVPLAEML GDMIWEDLSE CLIKNCLVYS IPTNSSKLQQ
YEEIIQSTEE FENALKEMRF LKGDTTDLLK YARNINSHFA NKKCQDVIVA ARNLMTSEIH
NTVKIIPDSK INVPELPTPD EDNKLEVQKV SNTQYHEVMN LEPENTLDQH SFSLPTCRIS
ESVKKLMELA YQTLLEATTS SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LQKLPQLAAI
HHNNCMYIAH HLLTLGHQFR LRLAPILCDG TATFVDLVPG FRRLGTECFL AQMRAQKGEL
LERLSSARNF SNMDDEENYS AASKAVRQVL HQLKRLGIVW QDVLPVNIYC KAMGTLLNTA
ISEVIGKITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL SEESKNKKYQ EEVPVYVPKW
MPFKELMMML QASLQEIGDR WADGKGPLAA AFSSSEVKAL IRALFQNTER RAAALAKIK
//

MIM 603954
*RECORD*
*FIELD* NO
603954
*FIELD* TI
*603954 ZESTE-WHITE 10, DROSOPHILA, HOMOLOG OF; ZW10
*FIELD* TX

CLONING

Mutations in the Drosophila zeste-white 10 (zw10) gene disrupt
read morechromosome segregation, producing chromosomes that lag at the metaphase
plate during anaphase of mitosis and both meiotic divisions. During the
cell cycle, the Drosophila zw10 protein moves from the
centromere/kinetochore at prometaphase to kinetochore microtubules at
metaphase, and then back to the centromere/kinetochore at anaphase.
Starr et al. (1997) suggested that zw10 may act at the kinetochore as
part of a tension-sensing checkpoint that renders anaphase onset
dependent upon bipolar tension exerted across all centromeres. By
screening an EST database, they identified cDNAs encoding zw10 homologs
in organisms with divergent centromere structures, including human, C.
elegans, and Arabidopsis. Overall, the predicted ZW10 proteins of
Drosophila, C. elegans, human, and Arabidopsis share 17 to 26% identity.
Northern blot analysis revealed that the human ZW10 gene was expressed
as an approximately 2.9-kb mRNA in various human cell lines. On Western
blots of HeLa cell extracts, the deduced 779-amino acid human ZW10
protein migrated as a doublet of approximately 90 kD. Using
immunofluorescence, the authors determined that ZW10 displayed a dynamic
pattern of localization during the HeLa cell cycle, similar to that
observed for Drosophila zw10.

GENE FUNCTION

Starr et al. (1997) found that injection of antisense C. elegans zw10
RNA into nematode gonads caused cell division disruptions similar to
those seen in Drosophila zw10 mutants. They concluded that at least some
aspects of the functional role of the ZW10 protein in ensuring proper
chromosome segregation are conserved in higher eukaryotes.

By coimmunoprecipitation of HeLa cells, Chan et al. (2000) and Scaerou
et al. (2001) determined that KNTC1 (607363) interacts with ZW10. Using
a yeast 2-hybrid screen, Scaerou et al. (2001) localized the
ZW10-binding region to the middle third of KNTC1. By immunofluorescent
localization in synchronized HeLa cells, Scaerou et al. (2001) found
KNTC1 and ZW10 colocalized at kinetochores during prometaphase and
metaphase, with the brightest staining for both proteins on the spindle
near the poles. After late anaphase and telophase, ZW10 accumulated at
the spindle midzone, while KNTC1 staining became prominent at the
spindle poles. By injection of antibodies directed toward KNTC1 or ZW10
into HeLa cells shortly after their release from the G1/S boundary, Chan
et al. (2000) found that anaphase cells contained lagging chromosomes
and divided cells contained chromatin bridges. They concluded that both
KNTC1 and ZW10 are essential components of the mitotic checkpoint.

Using yeast 2-hybrid analysis, Starr et al. (2000) demonstrated a direct
interaction between ZWINT (609177) and ZW10. The C-terminal third of
ZW10 interacted with ZWINT, but the full-length protein showed a tighter
association. At prophase, ZWINT localized to the kinetochore prior to
ZW10 localization. Starr et al. (2000) hypothesized that ZWINT may play
a role in targeting ZW10 to the kinetochore at prometaphase.

Musio et al. (2004) found that inhibition of INCENP (604411), ZWINT, and
ZW10 with antisense oligonucleotides resulted in the appearance of
mitotic cells characterized by centromere separation, chromosome
aneuploidy, and micronuclei formation. The chromosome morphology was
similar to that of Roberts syndrome (268300) chromosomes when analyzed
by atomic force microscopy.

MAPPING

The International Radiation Hybrid Mapping Consortium mapped the ZW10
gene to chromosome 11 (TMAP RH12322).

*FIELD* RF
1. Chan, G. K. T.; Jablonski, S. A.; Starr, D. A.; Goldberg, M. L.;
Yen, T. J.: Human Zw10 and ROD are mitotic checkpoint proteins that
bind to kinetochores. Nature Cell Biol. 2: 944-947, 2000.

2. Musio, A.; Mariani, T.; Montagna, C.; Zambroni, D.; Ascoli, C.;
Ried, T.; Vezzoni, P.: Recapitulation of the Roberts syndrome cellular
phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. Gene 331:
33-40, 2004.

3. Scaerou, F.; Starr, D. A.; Piano, F.; Papoulas, O.; Karess, R.
E.; Goldberg, M. L.: The ZW10 and rough deal checkpoint proteins
function together in a large, evolutionarily conserved complex targeted
to the kinetochore. J. Cell Sci. 114: 3103-3114, 2001.

4. Starr, D. A.; Saffery, R.; Li, Z.; Simpson, A. E.; Choo, K. H.
A.; Yen, T. J.; Goldberg, M. L.: HZwint-1, a novel human kinetochore
component that interacts with HZW10. J. Cell Sci. 113: 1939-1950,
2000.

5. Starr, D. A.; Williams, B. C.; Li, Z.; Etemad-Moghadam, B.; Dawe,
R. K.; Goldberg, M. L.: Conservation of the centromere/kinetochore
protein ZW10. J. Cell Biol. 138: 1289-1301, 1997.

*FIELD* CN
Patricia A. Hartz - updated: 01/28/2005
Patricia A. Hartz - updated: 11/19/2002

*FIELD* CD
Rebekah S. Rasooly: 6/30/1999

*FIELD* ED
mgross: 01/28/2005
mgross: 11/20/2002
mgross: 11/19/2002
mgross: 6/30/1999

RBCC Red Blood Cell Collection

Full text data of ZW10